Trh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trh
Entrez Gene ID	22044
Full Name	thyrotropin releasing hormone
Synonyms	Pro-TRH,Trf
General protein information	Preferred Names thyrotropin releasing hormone Names thyrotropin releasing hormone TSH-releasing factor pro-thyrotropin-releasing hormone prothyroliberin protirelin thyrotropin-releasing factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 D1\|6 41.03 cM
Summary	This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009426.3	NP_033452.2	thyrotropin releasing hormone preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRH	NP_009048.1
	TRH	XP_001093550.2
Mus musculus (house mouse)	Trh	NP_033452.2
Rattus norvegicus (Norway rat)	Trh	NP_037178.1
Pan troglodytes (chimpanzee)	TRH	XP_001144565.1
Bos taurus (cattle)	TRH	NP_001094691.1
Canis lupus familiaris (dog)	TRH	XP_005632182.1

Gene Ontology
Bibliography

Related articles in PubMed

Oxytocin signaling in the medial amygdala is required for sex discrimination of social cues.
Yao S, Bergan J, Lanjuin A, Dulac C
eLife6(2017 Dec)

Abnormal Paraventricular Nucleus of Hypothalamus and Growth Retardation Associated with Loss of Nuclear Receptor Gene COUP-TFII.
Feng S, Xing C, Shen T, Qiao Y, Wang R, Chen J, Liao J, Lu Z, Yang X, Abd-Allah SM, Li J, Jing N, Tang K
Scientific reports7(1)5282(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

co-regulation of the enzyme glutaminyl cyclase and its substrate TRH was reflected by a co-induction of both proteins in reactive astrocytes in proximity of Abeta deposits
Title: Identification of thyrotropin-releasing hormone as hippocampal glutaminyl cyclase substrate in neurons and reactive astrocytes.

TGFbeta2 regulates hypothalamic TRH expression through TIEG1 during fetal development.
Title: TGF?2 regulates hypothalamic Trh expression through the TGF? inducible early gene-1 (TIEG1) during fetal development.

RXRA and RXRB display specific roles in modulating T3-dependent regulation of thyrotropin releasing hormone.
Title: Retinoic X receptor subtypes exert differential effects on the regulation of Trh transcription.

Data suggest that expression of TRH is up-regulated by CREM (cAMP responsive element modulator protein) in hypothalamus; however, regulation of TRH by triiodothyronine appears independent of CREM or CREB (cAMP responsive element binding protein).
Title: Family members CREB and CREM control thyrotropin-releasing hormone (TRH) expression in the hypothalamus.

Thyrotropin-releasing hormone altered the basal expression of the TSHbeta gene and the responsiveness of the gene to thyroid hormone.
Title: NR4A1 (Nur77) mediates thyrotropin-releasing hormone-induced stimulation of transcription of the thyrotropin ? gene: analysis of TRH knockout mice.

The following Trh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23260	NM_009426.3 Latest version!	Mus musculus thyrotropin releasing hormone (Trh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trh ( NM_009426.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033452.2 Mus musculus thyrotropin releasing hormone (Trh), mRNA.

Price & Availability↑

CloneID	OMu23260
Clone ID Related Accession (Same CDS sequence)	NM_009426.3
Accession Version	NM_009426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	thyrotropin releasing hormone preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC164642.3, AK010666.1, CF951860.1 and AI836004.1. On May 1, 2013 this sequence version replaced NM_009426.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053493.1, AK010666.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164131, SAMN01164139 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGAC CTTGGCTGAT GATGGCTCTG GCTTTGATCT TCGTGCTAAC TGGTATCCCC 
AAATCCTGCG CCTTGCTGGA AGCAGCCCAG GAGGAAGGTG CTGTGACTCC TGACCTTCCA 
GGCCTGGAGA AAGTCCAGGT CCGGCCAGAA CGTCGATTCT TGAGGAAAGA CCTCCAGCGT 
GTGCGAGGGG ACCTTGGTGC TGCCTTAGAT TCCTGGATCA CAAAACGCCA GCATCCGGGC 
AAAAGGGAGG AGAAGGAGGA AGACGTTGAA GCCGAAGAGA GGGGAGATTT GGGAGAAGTG 
GGAGCCTGGA GACCCCACAA ACGACAGCAC CCTGGCCGAC GTGCCAACCA AGACAAGGAT 
TCATGGTCAG ATGAGGGGGA CAGTGACTGG CTGCCCCCGT CCTGGCTACC AGATTTCTTT 
CTGGATTCCT GGTTCTCAGA TGCCCCCCAA GTCAAGCGGC AGCACCCTGG CAGGCGATCC 
TTCCCCTGGA TGGAGTCTGA TGTCACCAAG AGGCAGCATC CAGGCCGGAG GTTCATAGAT 
CCTGAGCTCC AAAGAAGCTG GGAAGAAACA GAGGGAGAGG AGGGTGGCTT AATGCCTGAG 
AAACGTCAGC ATCCTGGCAA AAGGGCAGTG GGTCACCCTT GTGGGCCCCA GGGGATTTGT 
GGCCAAACAG GCCTGCTCCA GCTTCTAGGT GACCTGAGTA GGGGCCAGGA GACCCTGGCA 
AAGCAAACCC CACAACTGGA AGCCTGGGTC AGGGAACCTC TGGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033452.2
CDS	112..882
Translation	MQGPWLMMALALIFVLTGIPKSCALLEAAQEEGAVTPDLPGLEKVQVRPERRFLRKDLQRVRGDLGAALDSWITKRQHPGKREEKEEDVEAEERGDLGEVGAWRPHKRQHPGRRANQDKDSWSDEGDSDWLPPSWLPDFFLDSWFSDAPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEETEGEEGGLMPEKRQHPGKRAVGHPCGPQGICGQTGLLQLLGDLSRGQETLAKQTPQLEAWVREPLEE

Target ORF information:

RefSeq Version	NM_009426.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyrotropin releasing hormone (Trh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009426.3

ORF Insert Sequence:

ATGCAGGGAC CTTGGCTGAT GATGGCTCTG GCTTTGATCT TCGTGCTAAC TGGTATCCCC 
AAATCCTGCG CCTTGCTGGA AGCAGCCCAG GAGGAAGGTG CTGTGACTCC TGACCTTCCA 
GGCCTGGAGA AAGTCCAGGT CCGGCCAGAA CGTCGATTCT TGAGGAAAGA CCTCCAGCGT 
GTGCGAGGGG ACCTTGGTGC TGCCTTAGAT TCCTGGATCA CAAAACGCCA GCATCCGGGC 
AAAAGGGAGG AGAAGGAGGA AGACGTTGAA GCCGAAGAGA GGGGAGATTT GGGAGAAGTG 
GGAGCCTGGA GACCCCACAA ACGACAGCAC CCTGGCCGAC GTGCCAACCA AGACAAGGAT 
TCATGGTCAG ATGAGGGGGA CAGTGACTGG CTGCCCCCGT CCTGGCTACC AGATTTCTTT 
CTGGATTCCT GGTTCTCAGA TGCCCCCCAA GTCAAGCGGC AGCACCCTGG CAGGCGATCC 
TTCCCCTGGA TGGAGTCTGA TGTCACCAAG AGGCAGCATC CAGGCCGGAG GTTCATAGAT 
CCTGAGCTCC AAAGAAGCTG GGAAGAAACA GAGGGAGAGG AGGGTGGCTT AATGCCTGAG 
AAACGTCAGC ATCCTGGCAA AAGGGCAGTG GGTCACCCTT GTGGGCCCCA GGGGATTTGT 
GGCCAAACAG GCCTGCTCCA GCTTCTAGGT GACCTGAGTA GGGGCCAGGA GACCCTGGCA 
AAGCAAACCC CACAACTGGA AGCCTGGGTC AGGGAACCTC TGGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oxytocin signaling in the medial amygdala is required for sex discrimination of social cues.
eLife6(2017 Dec)
Yao S,Bergan J,Lanjuin A,Dulac C

Abnormal Paraventricular Nucleus of Hypothalamus and Growth Retardation Associated with Loss of Nuclear Receptor Gene COUP-TFII.
Scientific reports7(1)5282(2017 Jul)
Feng S,Xing C,Shen T,Qiao Y,Wang R,Chen J,Liao J,Lu Z,Yang X,Abd-Allah SM,Li J,Jing N,Tang K