Tnfaip6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnfaip6
Entrez Gene ID	21930
Full Name	tumor necrosis factor alpha induced protein 6
Synonyms	TSG-6,Tnfip6,Tsg6
General protein information	Preferred Names tumor necrosis factor alpha induced protein 6 Names tumor necrosis factor-inducible gene 6 protein TNF alpha-induced protein 6 TNF-stimulated gene 6 protein tumor necrosis factor induced protein 6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_009398.2	NP_033424.1	tumor necrosis factor-inducible gene 6 protein precursor

Pathways from BioSystems

Homologies

	TNFAIP6	XP_001083111.1
Homo sapiens (human)	TNFAIP6	NP_009046.2
Bos taurus (cattle)	TNFAIP6	NP_001007814.1
Mus musculus (house mouse)	Tnfaip6	NP_033424.1
Rattus norvegicus (Norway rat)	Tnfaip6	NP_445834.1
Canis lupus familiaris (dog)	TNFAIP6	XP_533354.3
Gallus gallus (chicken)	TNFAIP6	NP_001032926.2
Xenopus tropicalis (tropical clawed frog)	tnfaip6	XP_002936705.1
Pan troglodytes (chimpanzee)	TNFAIP6	XP_516218.2
Danio rerio (zebrafish)	tnfaip6	NP_001035333.2

Gene Ontology
Bibliography

Related articles in PubMed

TNF-stimulated gene 6 promotes formation of hyaluronan-inter-?-inhibitor heavy chain complexes necessary for ozone-induced airway hyperresponsiveness.
Stober VP, Johnson CG, Majors A, Lauer ME, Cali V, Midura RJ, Wisniewski HG, Aronica MA, Garantziotis S
The Journal of biological chemistry292(51)20845-20858(2017 Dec)

Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
Gesteira TF, Sun M, Coulson-Thomas YM, Yamaguchi Y, Yeh LK, Hascall V, Coulson-Thomas VJ
Investigative ophthalmology & visual science58(11)4407-4421(2017 Sep)

Tumor necrosis factor-inducible gene 6 protein ameliorates chronic liver damage by promoting autophagy formation in mice.
Wang S, Lee C, Kim J, Hyun J, Lim M, Cha HJ, Oh SH, Choi YH, Jung Y
Experimental & molecular medicine49(9)e380(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provided the first evidence of TSG-6 secreted by mesenchymal stem cells promoting corneal epithelial wound healing in diabetic mice through activating corneal epithelial stem/progenitor cells and accelerating M2 macrophage polarization
Title: Mesenchymal Stem Cells Promote Diabetic Corneal Epithelial Wound Healing Through TSG-6-Dependent Stem Cell Activation and Macrophage Switch.

This study shows that TSG-6 is expressed in the CNS, suggesting a role for TSG-6 in astrocyte activation and tissue repair.
Title: Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Is Constitutively Expressed in Adult Central Nervous System (CNS) and Associated with Astrocyte-mediated Glial Scar Formation following Spinal Cord Injury.

Data (including data from studies in knockout mice) suggest that TSG-6 is needed for release of hyaluronan rafts (leukocyte-adhesive rafts) from apical surface of tracheal mucosa into bronchoalveolar lavage fluid as heavy chain hyaluronan complexes.
Title: Hyaluronan Rafts on Airway Epithelial Cells.

the well characterized hyaluronan-binding site in the TSG-6 Link module is not used for recognition during transfer of HCs onto HA.
Title: Metal Ion-dependent Heavy Chain Transfer Activity of TSG-6 Mediates Assembly of the Cumulus-Oocyte Matrix.

Thus, TSG6, HA, and IalphaI were crucial factors for the settlement and probably the subsequent differentiation of MSCs.
Title: Acute and temporal expression of tumor necrosis factor (TNF)-?-stimulated gene 6 product, TSG6, in mesenchymal stem cells creates microenvironments required for their successful transplantation into muscle tissue.

The following Tnfaip6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnfaip6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15917	NM_009398.2 Latest version!	Mus musculus tumor necrosis factor alpha induced protein 6 (Tnfaip6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnfaip6 ( NM_009398.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033424.1 Mus musculus tumor necrosis factor alpha induced protein 6 (Tnfaip6), mRNA.

Price & Availability↑

CloneID	OMu15917
Clone ID Related Accession (Same CDS sequence)	NM_009398.2
Accession Version	NM_009398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	tumor necrosis factor-inducible gene 6 protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929138.15. On Nov 17, 2006 this sequence version replaced NM_009398.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U83903.1, AK139985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTCGTCC TCCTTTGCTT ATGCGTCTTG CTGTGGGAAG AGGCTCACGG ATGGGGATTC 
AAGAACGGGA TCTTTCATAA CTCCATATGG CTTGAACAAG CAGCGGGCGT ATACCACAGA 
GAAGCTCGGG CTGGCAGATA CAAGCTCACC TACGCCGAAG CCAAGGCCGT ATGTGAATTT 
GAAGGTGGTC GTCTCGCAAC CTACAAGCAG CTAGAGGCAG CCAGAAAAAT TGGATTCCAT 
GTCTGTGCTG CTGGATGGAT GGCCAAGGGT AGAGTCGGAT ACCCCATTGT GAAACCTGGG 
CCCAACTGTG GATTTGGGAA AACGGGTATC ATCGATTATG GAATCCGGCT CAACAGGAGT 
GAGCGATGGG ATGCCTATTG CTACAACCCA CATGCAAAGG AGTGTGGTGG TGTCTTCACA 
GATCCGAAGC GAATTTTTAA ATCCCCGGGC TTCCCAAATG AGTACGATGA CAACCAGGTC 
TGCTACTGGC ACATTCGGCT CAAGTACGGT CAGCGAATTC ACCTGAGCTT TTTGGACTTT 
GACCTTGAAC ATGATCCAGG CTGCTTGGCT GACTATGTAG AAATCTATGA CAGTTATGAT 
GACGTCCACG GCTTTGTAGG AAGATACTGT GGTGATGAAC TTCCAGAAGA CATCATTAGC 
ACAGGAAATG TCATGACCTT GAAGTTTCTG AGTGATGCAT CCGTCACGGC TGGAGGCTTC 
CAGATTAAAT ACGTCACAGT GGATCCTGCA TCTAAATCCA GTCAAGCCAA AAATACAAGT 
ACTACTGGAA ATAAGAAGTT CTTACCTGGA AGGTTTAGCC ATCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033424.1
CDS	64..891
Translation	MVVLLCLCVLLWEEAHGWGFKNGIFHNSIWLEQAAGVYHREARAGRYKLTYAEAKAVCEFEGGRLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKRIFKSPGFPNEYDDNQVCYWHIRLKYGQRIHLSFLDFDLEHDPGCLADYVEIYDSYDDVHGFVGRYCGDELPEDIISTGNVMTLKFLSDASVTAGGFQIKYVTVDPASKSSQAKNTSTTGNKKFLPGRFSHL

Target ORF information:

RefSeq Version	NM_009398.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tumor necrosis factor alpha induced protein 6 (Tnfaip6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009398.2

ORF Insert Sequence:

ATGGTCGTCC TCCTTTGCTT ATGCGTCTTG CTGTGGGAAG AGGCTCACGG ATGGGGATTC 
AAGAACGGGA TCTTTCATAA CTCCATATGG CTTGAACAAG CAGCGGGCGT ATACCACAGA 
GAAGCTCGGG CTGGCAGATA CAAGCTCACC TACGCCGAAG CCAAGGCCGT ATGTGAATTT 
GAAGGTGGTC GTCTCGCAAC CTACAAGCAG CTAGAGGCAG CCAGAAAAAT TGGATTCCAT 
GTCTGTGCTG CTGGATGGAT GGCCAAGGGT AGAGTCGGAT ACCCCATTGT GAAACCTGGG 
CCCAACTGTG GATTTGGGAA AACGGGTATC ATCGATTATG GAATCCGGCT CAACAGGAGT 
GAGCGATGGG ATGCCTATTG CTACAACCCA CATGCAAAGG AGTGTGGTGG TGTCTTCACA 
GATCCGAAGC GAATTTTTAA ATCCCCGGGC TTCCCAAATG AGTACGATGA CAACCAGGTC 
TGCTACTGGC ACATTCGGCT CAAGTACGGT CAGCGAATTC ACCTGAGCTT TTTGGACTTT 
GACCTTGAAC ATGATCCAGG CTGCTTGGCT GACTATGTAG AAATCTATGA CAGTTATGAT 
GACGTCCACG GCTTTGTAGG AAGATACTGT GGTGATGAAC TTCCAGAAGA CATCATTAGC 
ACAGGAAATG TCATGACCTT GAAGTTTCTG AGTGATGCAT CCGTCACGGC TGGAGGCTTC 
CAGATTAAAT ACGTCACAGT GGATCCTGCA TCTAAATCCA GTCAAGCCAA AAATACAAGT 
ACTACTGGAA ATAAGAAGTT CTTACCTGGA AGGTTTAGCC ATCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TNF-stimulated gene 6 promotes formation of hyaluronan-inter-?-inhibitor heavy chain complexes necessary for ozone-induced airway hyperresponsiveness.
The Journal of biological chemistry292(51)20845-20858(2017 Dec)
Stober VP,Johnson CG,Majors A,Lauer ME,Cali V,Midura RJ,Wisniewski HG,Aronica MA,Garantziotis S

Hyaluronan Rich Microenvironment in the Limbal Stem Cell Niche Regulates Limbal Stem Cell Differentiation.
Investigative ophthalmology & visual science58(11)4407-4421(2017 Sep)
Gesteira TF,Sun M,Coulson-Thomas YM,Yamaguchi Y,Yeh LK,Hascall V,Coulson-Thomas VJ

Tumor necrosis factor-inducible gene 6 protein ameliorates chronic liver damage by promoting autophagy formation in mice.
Experimental & molecular medicine49(9)e380(2017 Sep)
Wang S,Lee C,Kim J,Hyun J,Lim M,Cha HJ,Oh SH,Choi YH,Jung Y