Kdm6b gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006532898.1	XP_006532961.1	lysine-specific demethylase 6B isoform X1
XM_011248922.2	XP_011247224.1	lysine-specific demethylase 6B isoform X1
XM_030245821.1	XP_030101681.1	lysine-specific demethylase 6B isoform X1
XM_030245825.1	XP_030101685.1	lysine-specific demethylase 6B isoform X1
XM_011248920.2	XP_011247222.1	lysine-specific demethylase 6B isoform X1
XM_011248923.1	XP_011247225.1	lysine-specific demethylase 6B isoform X1
NM_001017426.1	NP_001017426.1	lysine-specific demethylase 6B
XM_030245818.1	XP_030101678.1	lysine-specific demethylase 6B isoform X1
XM_030245819.1	XP_030101679.1	lysine-specific demethylase 6B isoform X1
XM_030245824.1	XP_030101684.1	lysine-specific demethylase 6B isoform X1
NM_001017426.2	NP_001017426.1	lysine-specific demethylase 6B
XM_030245820.1	XP_030101680.1	lysine-specific demethylase 6B isoform X1
XM_030245823.1	XP_030101683.1	lysine-specific demethylase 6B isoform X1
XM_011248921.1	XP_011247223.1	lysine-specific demethylase 6B isoform X1
XM_030245816.1	XP_030101676.1	lysine-specific demethylase 6B isoform X1
XM_030245817.1	XP_030101677.1	lysine-specific demethylase 6B isoform X1
XM_011248919.2	XP_011247221.1	lysine-specific demethylase 6B isoform X1
XM_030245822.1	XP_030101682.1	lysine-specific demethylase 6B isoform X1
XM_030245826.1	XP_030101686.1	lysine-specific demethylase 6B isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu85727	XM_030245820.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245822.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245823.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245816.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245824.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245825.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245818.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245819.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245826.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245817.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85727	XM_030245821.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu16573	NM_001017426.2 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu16573	XM_011248921.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu16573	XM_006532898.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu16573	XM_011248920.2 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu16573	XM_011248923.1 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu16573	XM_011248922.2 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu16573	XM_011248919.2 Latest version!	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OMu16573	NM_001017426.1	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
Hide Old Accession Versions >>

Kdm6b ( XM_030245821.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101681.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101681.1
CDS	606..5534
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245821.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245821.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245825.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101685.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X10, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101685.1
CDS	711..5639
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245825.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245825.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245818.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101678.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101678.1
CDS	478..5406
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245818.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245818.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245819.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101679.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X4, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101679.1
CDS	712..5640
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245819.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245819.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245824.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101684.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X9, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101684.1
CDS	433..5361
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245824.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245824.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245820.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101680.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X5, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101680.1
CDS	540..5468
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245820.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245823.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101683.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X8, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101683.1
CDS	1114..6042
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245823.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245823.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245816.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101676.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X1, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101676.1
CDS	742..5670
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245816.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245816.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245817.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101677.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101677.1
CDS	556..5484
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245817.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245817.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245822.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101682.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X7, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101682.1
CDS	1334..6262
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245822.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245822.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_030245826.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101686.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X11, mRNA.

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CloneID	OMu85727
Clone ID Related Accession (Same CDS sequence)	XM_030245821.1 , XM_030245825.1 , XM_030245818.1 , XM_030245819.1 , XM_030245824.1 , XM_030245820.1 , XM_030245823.1 , XM_030245816.1 , XM_030245817.1 , XM_030245822.1 , XM_030245826.1
Accession Version	XM_030245826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101686.1
CDS	239..5167
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_030245826.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245826.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGCAGGAGG AGAGGGAGAG TGAGGATGAG 
GAATCCGAGG AACCAGACAG CACTACAGGA ACCTCTCCCA GCAGTGCACC GGACCCCAAG 
AACCATCACA TCATCAAGTT TGGCACTAAC ATCGACCTGT CTGATGCCAA GAGGTGGAAG 
CCACAGCTAC AGGAGCTGCT GAAACTGCCC GCCTTCATGC GGGTAACATC CACAGGCAAC 
ATGCTCAGCC ACGTGGGCCA CACCATCCTG GGCATGAACA CCGTGCAGCT ATACATGAAG 
GTCCCTGGCA GCCGAACGCC AGGCCACCAA GAGAATAACA ATTTCTGCTC AGTCAACATC 
AACATTGGCC CTGGGGACTG CGAGTGGTTC GCGGTACATG AGCACTATTG GGAGACCATC 
AGCGCCTTCT GCGACCGGCA TGGTGTGGAC TACTTGACTG GTTCCTGGTG GCCAATCTTG 
GATGACCTCT ATGCGTCCAA TATTCCTGTT TACCGCTTCG TGCAGCGCCC TGGAGACCTT 
GTGTGGATTA ATGCAGGGAC TGTACATTGG GTGCAGGCTA CCGGCTGGTG CAACAACATT 
GCCTGGAACG TGGGGCCCCT CACCGCCTAT CAGTACCAGC TGGCCCTGGA GCGATATGAG 
TGGAACGAGG TGAAGAACGT CAAGTCCATT GTGCCCATGA TTCATGTGTC CTGGAACGTC 
GCTCGAACGG TCAAGATCAG CGATCCTGAC TTGTTCAAGA TGATCAAGTT CTGCCTCCTG 
CAGTCAATGA AGCACTGTCA GGTACAGCGG GAGAGCCTGG TGCGGGCAGG GAAGAAGATC 
GCTTACCAAG GCCGTGTCAA AGACGAGCCT GCCTACTACT GCAACGAATG CGACGTGGAG 
GTGTTCAACA TCCTGTTCGT TACAAGTGAG AATGGCAGCC GAAACACGTA CCTGGTGCAC 
TGCGAGGGCT GTGCGCGCCG TCGCAGCGCG GGCCTACAGG GCGTGGTGGT GCTAGAGCAG 
TACCGCACGG AGGAGCTGGC GCAGGCCTAC GATGCCTTCA CACTGGCTCC CGCCAGCACG 
TCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( NM_001017426.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001017426.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	NM_001017426.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075632.1, CA945559.1 and AL596125.27. On May 17, 2005 this sequence version replaced XM_126426.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC075632.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017426.1
CDS	411..5336
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	NM_001017426.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017426.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( NM_001017426.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001017426.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	NM_001017426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596125.27. On or before Jun 8, 2018 this sequence version replaced XM_006532898.1, NM_001017426.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075632.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017426.1
CDS	587..5512
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	NM_001017426.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017426.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_006532898.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532961.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X1, mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	XM_006532898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532961.1
CDS	582..5507
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_006532898.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532898.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_011248922.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247224.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	XM_011248922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247224.1
CDS	802..5727
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_011248922.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248922.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_011248920.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247222.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X3, mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	XM_011248920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247222.1
CDS	718..5643
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_011248920.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248920.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_011248923.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247225.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X6, mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	XM_011248923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247225.1
CDS	885..5810
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_011248923.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248923.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_011248921.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247223.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X4, mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	XM_011248921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247223.1
CDS	892..5817
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_011248921.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248921.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6b ( XM_011248919.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247221.1 Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X2, mRNA.

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CloneID	OMu16573
Clone ID Related Accession (Same CDS sequence)	XM_006532898.1 , XM_011248922.2 , XM_011248920.2 , XM_011248923.1 , NM_001017426.1 , NM_001017426.2 , XM_011248921.1 , XM_011248919.2
Accession Version	XM_011248919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247221.1
CDS	485..5410
Translation	MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSFAELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMPPAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTKPGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHGCLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSPGIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWMKGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSSNSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESPRPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEEKKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYSPSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPARSESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKEHRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAPPPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_011248919.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus KDM1 lysine (K)-specific demethylase 6B (Kdm6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248919.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCTCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC TTGGAGCTCC TGCCCACCCC ATCCTCCTCC CCGAAGCTCA 
TGGCTGCCCG GAGGCAGATG CTCTGCCAGC GTTGGGCAGC CCCCACTCTC AGCTCCTTTA 
CCCCCATCTC ATGGCAGTAG CTCCGGGCAC CCTAACAAAC CCTATTATGC TCCTGGGACA 
CCCACCCCAA GACCCCTTCA CGGGAAGTTG GAATCCCTAC ATGGCTGTGT CCAGGCATTG 
CTCCGGGAGC CAGCGCAGCC AGGGTTGTGG GAACAGCTTG GACAGTTGTA TGAATCAGAG 
CACGACAGTG AGGAAGCCGT ATGCTGCTAC CATAGGGCCC TTCGCTATGG AGGAAGCTTC 
GCCGAGCTGG GACCCCGGAT TGGCCGCTTG CAGCAGGCCC AGCTCTGGAA CTTTCATGCC 
GGTTCCTGTC AGCACAGAGC CAAGGTCCTG CCTCCCCTGG AGCAAGTCTG GAATTTGCTG 
CACCTTGAGC ACAAACGGAA CTATGGGGCT AAGCGAGGGG GCCCTCCAGT GAAGAGATCT 
GCTGAACCCC CCGTGGTCCA GCCTATGCCT CCTGCAGCCC TCTCAGGCCC CTCAGGAGAG 
GAGGGCCTTA GCCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCTGAACA GGCTGGCCTT 
CCCCCAGGTC TGCCACTCCC TCCACCACCC CCACCCCCAC CGCCTCCACC ACCACCACCA 
CCCCCTCCAC CACCACCGCT GCCTGGCCTG GCTATTAGCC CCCCATTTCA GCTGACTAAG 
CCAGGGCTGT GGAATACCCT GCATGGAGAT GCTTGGGGCC CCGAGCGCAA GGGTTCAGCG 
CCGCCAGAGC GCCAGGAGCA GCGGCACTCG ATGCCTCATT CATATCCATA CCCAGCTCCC 
GCCTACTCCG CTCATCCGCC CAGCCATCGG CTGGTCCCCA ACACACCCCT TGGTCCAGGT 
CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCAGCAGC 
AGCAGCAGCA GCAGCAGTAG CAGTAACAAC ACTGGTCTTC GGGGTGTGGA GCCAAGCCCA 
GGCATTCCTG GCGCTGACCA TTACCAAAAC CCTGCGCTGG AGATATCCCC TCACCAGGCC 
CGCCTGGGTC CCTCCGCACA CAGCAGTCGG AAACCATTTT TGACGGCCCC TGCTGCCACG 
CCCCACTTAT CCCTACCCCC TGGGACCCCA TCATCCCCTC CACCCCCATG TCCTCGCCTC 
TTGCGCCCTC CACCGCCCCC TGCTTGGATG AAGGGCTCAG CCTGCCGTGC AGCCCGAGAG 
GATGGAGAGA TCTTAGGGGA GCTCTTCTTT GGTGCTGAGG GACCTCCCCG TCCTCCTCCC 
CCACCCCTTC CCCACCGTGA TGGCTTCTTG GGGCCTCCAA ACCCCCGCTT TTCTGTGGGC 
ACTCAGGATT CGCATAACCC TCCCATTCCC CCAACCACCA CCAGCAGCAG CAGCAGCAGC 
AACAGCCACA GCAGTAGTCC TACTGGGCCG GTGCCCTTTC CACCACCCTC CTATCTGGCC 
AGAAGTATAG ACCCCCTCCC CAGGCCATCC AGCCCAACCT TGAGCCCCCA GGACCCACCT 
CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT CCTCCTGCCA CCAAAATACC 
TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG TCTCCTTCCC AAAGACCCCC 
GAGGTGGGGC AGGGGCCACC CCCAGGCCCT GTGAGTAAAG CCCCCCAGCC TGTGCCACCT 
GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG ATTTCCCACC CACTCCGCTG 
GAGGACCAGT TTGAAGAGCC AGCCGAATTC AAGATCCTAC CTGATGGGCT GGCAAACATC 
ATGAAGATGC TGGATGAATC CATTCGGAAG GAGGAGGAGC AGCAGCAGCA GCAGGAGGCA 
GGCGTGGCTC CCCCACCCCC ACTCAAAGAG CCCTTTGCAT CTCTACAGCC TCCATTTCCC 
AGTGACACAG CCCCAGCCAC CACCACTGCT GCCCCCACCA CCGCCACCAC CACCACAACC 
ACCACCACCA CCACCACCCA AGAAGAGGAG AAGAAGCCAC CACCAGCCCT ACCACCACCA 
CCGCCTCTAG CCAAGTTTCC TCCACCTCCC CAGCCACAGC CCCCACCACC TCCACCAGCC 
AGCCCAGCCA GCCTGCTCAA ATCGTTGGCC TCTGTTCTTG AGGGACAAAA GTACTGTTAC 
CGGGGGACTG GAGCAGCCGT CTCAACCAGG CCCGGGTCCG TGCCCGCCAC TCAGTATTCC 
CCTAGTCCTG CATCAGGTGC TACCGCCCCA CCACCCACTT CAGTGGCCCC TAGTGCCCAG 
GGCTCCCCCA AGCCCTCGGT TTCCTCGTCA TCTCAGTTCT CTACCTCAGG CGGGCCTTGG 
GCCCGGGAGC ACAGGGCGGG TGAAGAGCCA GCACCAGGCC CCGTGACCCC TGCCCAGTTG 
CCCCCACCTC TGCCGCTGCC CCCTGCTCGT TCTGAGTCTG AGGTGCTAGA AGAAATCAGT 
CGGGCTTGTG AGACCCTTGT AGAGCGGGTG GGCCGGAGTG CCATCAACCC AGTGGACACG 
GCAGACCCAG TGGACAGTGG GACTGAGCCA CAGCCGCCGC CTGCGCAGGC CAAGGAGGAG 
AGTGGGGGGG TGGCGGTAGC AGCAGCAGGT CCAGGTAGTG GCAAGCGTCG TCAAAAGGAG 
CATCGGCGGC ACAGGCGGGC CTGTAGGGAC AGTGTGGGTC GACGACCCCG CGAGGGGAGG 
GCCAAGGCCA AGGCCAAGGC TCCCAAAGAA AAAAGCCGAA GGGTGCTGGG GAACCTCGAC 
TTGCAGAGTG AGGAGATCCA GGGCCGGGAG AAGGCCCGGC CCGATGTCGG TGGGGTTTCC 
AAAGTCAAGA CACCCACAGC TCCAGCACCC CCGCCTGCTC CTGCACCCGC TGCTCAGCCA 
ACACCCCCAT CAGCTCCTGT CCCTGGGAAG AAGACTCGTG AGGAGGCTCC GGGGCCTCCA 
GGTGTGAGCC GGGCAGATAT GCTGAAGCTC CGGTCACTTA GTGAGGGGCC TCCCAAGGAG 
CTGAAGATCA GGCTCATCAA GGTGGAAAGT GGGGACAAGG AGACCTTTAT CGCCTCTGAG 
GTGGAAGAGC GGCGGCTGCG CATGGCAGAC CTCACCATCA GCCACTGTGC CGCCGATGTC 
ATGCGTGCCA GCAAGAATGC CAAGGTGAAA GGGAAATTCC GAGAGTCCTA CCTGTCCCCT 
GCCCAGTCTG TGAAACCCAA GATCAACACT GAGGAGAAGC TGCCCCGGGA AAAACTCAAC 
CCCCCTACCC CCAGCATCTA TTTGGAGAGC AAACGAGATG CCTTCTCGCC GGTCCTGCTA 
CAGTTCTGTA CAGACCCCCG GAACCCCATC ACCGTCATCA GGGGCCTGGC TGGTTCACTT 
CGGCTCAACT TAGGCCTTTT CTCCACCAAG ACTCTGGTGG AGGCGAGCGG TGAACATACG 
GTGGAGGTCC GTACCCAAGT ACAGCAGCCC TCAGACGAGA ACTGGGACCT GACAGGTACC 
AGACAAATCT GGCCCTGTGA GAGCTCCCGT TCCCACACCA CCATCGCTAA ATACGCACAG 
TACCAGGCCT CGTCCTTCCA GGAGTCACTG CAGGAGGAGA GGGAGAGTGA GGATGAGGAA 
TCCGAGGAAC CAGACAGCAC TACAGGAACC TCTCCCAGCA GTGCACCGGA CCCCAAGAAC 
CATCACATCA TCAAGTTTGG CACTAACATC GACCTGTCTG ATGCCAAGAG GTGGAAGCCA 
CAGCTACAGG AGCTGCTGAA ACTGCCCGCC TTCATGCGGG TAACATCCAC AGGCAACATG 
CTCAGCCACG TGGGCCACAC CATCCTGGGC ATGAACACCG TGCAGCTATA CATGAAGGTC 
CCTGGCAGCC GAACGCCAGG CCACCAAGAG AATAACAATT TCTGCTCAGT CAACATCAAC 
ATTGGCCCTG GGGACTGCGA GTGGTTCGCG GTACATGAGC ACTATTGGGA GACCATCAGC 
GCCTTCTGCG ACCGGCATGG TGTGGACTAC TTGACTGGTT CCTGGTGGCC AATCTTGGAT 
GACCTCTATG CGTCCAATAT TCCTGTTTAC CGCTTCGTGC AGCGCCCTGG AGACCTTGTG 
TGGATTAATG CAGGGACTGT ACATTGGGTG CAGGCTACCG GCTGGTGCAA CAACATTGCC 
TGGAACGTGG GGCCCCTCAC CGCCTATCAG TACCAGCTGG CCCTGGAGCG ATATGAGTGG 
AACGAGGTGA AGAACGTCAA GTCCATTGTG CCCATGATTC ATGTGTCCTG GAACGTCGCT 
CGAACGGTCA AGATCAGCGA TCCTGACTTG TTCAAGATGA TCAAGTTCTG CCTCCTGCAG 
TCAATGAAGC ACTGTCAGGT ACAGCGGGAG AGCCTGGTGC GGGCAGGGAA GAAGATCGCT 
TACCAAGGCC GTGTCAAAGA CGAGCCTGCC TACTACTGCA ACGAATGCGA CGTGGAGGTG 
TTCAACATCC TGTTCGTTAC AAGTGAGAAT GGCAGCCGAA ACACGTACCT GGTGCACTGC 
GAGGGCTGTG CGCGCCGTCG CAGCGCGGGC CTACAGGGCG TGGTGGTGCT AGAGCAGTAC 
CGCACGGAGG AGCTGGCGCA GGCCTACGAT GCCTTCACAC TGGCTCCCGC CAGCACGTCT 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Kdm6b
Entrez Gene ID	216850
Full Name	KDM1 lysine (K)-specific demethylase 6B
Synonyms	1700064E03Rik,BC038313,Jmjd3,mKIAA0346
General protein information	Preferred Names KDM1 lysine (K)-specific demethylase 6B Names lysine-specific demethylase 6B jmjC domain-containing protein 3 jumonji domain containing 3 jumonji domain-containing protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B3

Mus musculus (house mouse)	Kdm6b	NP_001017426.1
Pan troglodytes (chimpanzee)	KDM6B	XP_003315411.1
Rattus norvegicus (Norway rat)	Kdm6b	NP_001102299.1
Canis lupus familiaris (dog)	KDM6B	XP_005620054.1
Bos taurus (cattle)	KDM6B	XP_003587460.2
Homo sapiens (human)	KDM6B	NP_001073893.1
	KDM6B	XP_001110616.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Kdm6b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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