Stk10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stk10
Entrez Gene ID	20868
Full Name	serine/threonine kinase 10
Synonyms	Gek1,Lok,mKIAA4026
General protein information	Preferred Names serine/threonine kinase 10 Names serine/threonine-protein kinase 10 germ cell kinase 1 lymphocyte-oriented kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 18.97 cM\|11 A4
Summary	This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001359178.1	NP_001346107.1	serine/threonine-protein kinase 10 isoform 3
NM_001359177.1	NP_001346106.1	serine/threonine-protein kinase 10 isoform 2
NM_009288.2	NP_033314.2	serine/threonine-protein kinase 10 isoform 1
XM_017314404.1	XP_017169893.1	serine/threonine-protein kinase 10 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	STK10	XP_546239.2
Bos taurus (cattle)	STK10	NP_001179556.1
Rattus norvegicus (Norway rat)	Stk10	NP_062079.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010551	XP_314524.4
Homo sapiens (human)	STK10	NP_005981.3
	STK10	XP_002804682.1
Gallus gallus (chicken)	STK10	XP_414529.4
Caenorhabditis elegans (roundworm)	gck-4	NP_510290.1
Pan troglodytes (chimpanzee)	STK10	XP_518098.4
Mus musculus (house mouse)	Stk10	NP_033314.2
Danio rerio (zebrafish)	stk10	NP_957427.1
Drosophila melanogaster (fruit fly)	slik	NP_001014549.1
Xenopus tropicalis (tropical clawed frog)	stk10	NP_001072623.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternal DNA Methylation Regulates Early Trophoblast Development.
Branco MR, King M, Perez-Garcia V, Bogutz AB, Caley M, Fineberg E, Lefebvre L, Cook SJ, Dean W, Hemberger M, Reik W
Developmental cell36(2)152-63(2016 Jan)

A combination of SILAC and nucleotide acyl phosphate labelling reveals unexpected targets of the Rsk inhibitor BI-D1870.
Edgar AJ, Trost M, Watts C, Zaru R
Bioscience reports34(1)(2014 Feb)

LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation.
Belkina NV, Liu Y, Hao JJ, Karasuyama H, Shaw S
Proceedings of the National Academy of Sciences of the United States of America106(12)4707-12(2009 Mar)

Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.
Pike AC, Rellos P, Niesen FH, Turnbull A, Oliver AW, Parker SA, Turk BE, Pearl LH, Knapp S
The EMBO journal27(4)704-14(2008 Feb)

Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue.
Walter SA, Cutler RE Jr, Martinez R, Gishizky M, Hill RJ
The Journal of biological chemistry278(20)18221-8(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

kinases, including Slk, Lok and Mst1, are inhibited by BI-D1870 but to a much lesser extent by BIX 02565 and that phosphorylation of some of their substrates is blocked by BI-D1870 in living cells.
Title: A combination of SILAC and nucleotide acyl phosphate labelling reveals unexpected targets of the Rsk inhibitor BI-D1870.

These studies identify a new ERM kinase of importance in lymphocytes and confirm the role of ezrin-radixin-moesin phosphorylation in regulating cell shape and motility.
Title: LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation.

Stk10 is a novel polo-like kinase kinase that cooperates with hSlk to regulate Plk1 function in human cells
Title: Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue.

The following Stk10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stk10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03881	NM_009288.2 Latest version!	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OMu77823	NM_001359177.1 Latest version!	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu83659	NM_001359178.1 Latest version!	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu77823	XM_017314404.1 Latest version!	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stk10 ( NM_009288.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033314.2 Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03881
Clone ID Related Accession (Same CDS sequence)	NM_009288.2
Accession Version	NM_009288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase 10 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY207613.1, BC128363.1, BC096624.1, AL669844.20 and AA182088.1. On Feb 28, 2007 this sequence version replaced NM_009288.1. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: D89728.1, AK148118.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCG CCAATTTCCG CCGCATCTTG CGGTTATCCA CCTTCGAGAA GAGAAAGTCC 
CGTGAATATG AGCACGTCCG CCGGGACCTG GACCCCAACG ATGTATGGGA GATCGTGGGC 
GAGCTGGGAG ACGGCGCCTT CGGCAAAGTC TACAAGGCCA AAAACAAGGA GACCGGTGCC 
CTGGCAGCAG CCAAAGTCAT AGAGACCAAG AGCGAGGAGG AGCTGGAGGA CTACATCGTG 
GAGATAGAGA TCCTGGCCAC CTGTGACCAC CCGTATATCG TGAAGCTCCT GGGGGCCTAT 
TACTATGATG GGAAGCTCTG GATCATGATT GAGTTCTGCC CTGGGGGAGC TGTGGATGCC 
ATCATGCTGG AGCTGGATCG AGGCCTCACT GAGCCCCAGA TTCAGGTCGT ATGCCGCCAG 
ATGTTGGAGG CTCTCAACTT CCTGCACGGC AAGAGGATCA TCCACCGAGA CCTGAAAGCT 
GGCAACGTGC TTATGACCCT TGAGGGGGAC ATCAGGCTGG CTGATTTTGG TGTGTCTGCC 
AAGAATCTGA AAACTCTGCA AAAGCGAGAC TCCTTCATAG GGACACCTTA CTGGATGGCC 
CCTGAGGTGG TGCTGTGTGA AACCATGAAG GACGCCCCCT ATGACTACAA GGCTGACATC 
TGGTCCTTGG GCATCACGCT GATCGAGATG GCGCAGATCG AGCCCCCACA CCACGAGCTC 
AACCCCATGC GGGTCCTGCT CAAGATTGCC AAGTCGGACC CTCCTACCCT GCTTACACCC 
TCCAAGTGGT CTGTGGAGTT CCGAGACTTT CTGAAGATAG CCTTGGATAA GAACCCAGAA 
ACCCGGCCCA GTGCTGCACA GCTGCTGCAG CATCCCTTCG TCAGCAGAGT CACCAGCAAT 
AAGGCTCTTC GGGAGCTGGT GGCCGAGGCA AAGGCGGAGG TAATGGAGGA GATTGAGGAT 
GGCAGGGAGG ACGGAGAAGA GGAGGATGCT GTGGATGCTG TTCCGCCCCT GGTCAACCAC 
ACTCAGGACT CTGCCAATGT AACTCAGCCA AGCCTCGACT CCAACAAGCT TCTCCAGGAT 
TCTTCCACCC CCCTGCCTCC CAGCCAGCCT CAGGAGCCTG TGAGCGGGTC CTGTAGCCAA 
CCCTCTGGGG ATGGACCCCT CCAAACCACC AGCCCTGCAG ATGGGCTCTC CAAGAATGAC 
AATGACTTAA AGGTGCCTGT TCCCCTCCGG AAGTCCCGGC CACTGTCCAT GGATGCCAGA 
ATTCAGATGG ATGAAGAGAA ACAAATCCCT GACCAGGATG AGAACCCGAG TCCTGCAGCC 
AGCAAGTCCC AAAAGGCCAA CCAGAGCCGA CCTAACAGCA GTGCCCTGGA GACCTTGGGT 
GGCGAGGCTC TGACCAACGG TGGCCTGGAA CTCCCCAGCT CTGTCACTCC AAGCCATTCT 
AAGAGGGCCT CGGACTGTAG CAACCTGTCT ACCTCAGAGA GCATGGACTA TGGCACCTCC 
CTGTCTGCTG ACCTGTCACT GAACAAAGAA ACGGGCTCAT TGTCTCTCAA GGGCTCAAAA 
CTGCACAATA AGACCCTGAA ACGGACCCGC CGGTTTGTGG TGGATGGTGT GGAGGTGAGC 
ATCACCACCT CCAAGATCAT CAGCGAGGAT GAGAAGAAAG ACGAGGAGAT GAGATTTCTC 
AGGCGCCAGG AGCTCCGAGA GCTTCGGCTT CTGCAGAAAG AAGAACATCG GAACCAGACG 
CAGCTGAGCA GCAAGCACGA GCTGCAGCTG GAGCAGATGC ACAAACGTTT TGAACAAGAA 
ATCAACGCCA AGAAGAAATT CTATGACGTG GAGCTAGAAA ACCTGGAGCG GCAGCAGAAG 
CAGCAGGTAG AGAAGATGGA GCAGGACCAC AGCGTGCGCC GCAAAGAGGA GGCCAAGCGG 
ATCCGCCTGG AGCAGGATCG AGACTACGCC AAGTTCCAAG AGCAACTCAA GCAAATGAAG 
AAGGAGGTGA AGAGTGAGGT TGAGAAACTG CCCCGGCAGC AGCGGAAGGA GAGCATGAAG 
CAGAAGATGG AGGAACACTC TCAGAAGAAA CAGCGGCTGG ACCGGGACTT TGTAGCCAAG 
CAGAAGGAGG ACCTGGAGCT GGCCATGAGG AAGCTCACCA CGGAAAACAG GCGTGAGATC 
TGCGACAAGG AACGAGATTG CCTTAGCAAG AAGCAGGAGC TCCTCCGAGA CCGTGAGGCG 
GCCCTGTGGG AGATGGAGGA GCACCAGTTG CAGGAGCGAC ATCAGCTGGT GAAGCAGCAA 
CTGAAGGACC AGTACTTCCT GCAGCGGCAT GACCTGCTGC GTAAGCATGA GAAGGAGCGG 
GAACAGATGC AGCGCTACAA CCAGCGCATG ATGGAGCAGC TGAAGGTCAG ACAGCAACAG 
GAGAAGGCGC GGCTACCCAA GATCCAGAGG AGCGACGGCA AGACGCGCAT GGCCATGTAC 
AAGAAGAGCC TGCACATCAA CGGTGCGGGC AGTGCCTCTG AGCAGCGCGA GAAGATCAAG 
CAGTTCTCCC AGCAGGAGGA GAAGAGGCAG AAGGCGGAGA GGCTGCAGCA GCAGCAGAAA 
CACGAGAACC AGATGCGAGA CATGGTGGCG CAGTGTGAAA GCAACATGAG CGAGCTACAG 
CAGCTGCAGA ATGAAAAGTG TCATCTGTTG GTAGAGCATG AAACCCAGAA GCTGAAGGCC 
CTGGATGAGA GCCACAACCA GAGCCTGAAG GAATGGCGAG ACAAGCTTCG GCCACGCAAA 
AAGGCCCTGG AAGAGGATTT GAACCAGAAG AAGCGGGAAC AGGAAATGTT CTTCAAATTA 
AGCGAGGAGG CAGAGCCCAG ACCCACCACA CCCAGCAAAG CCAGCAACTT CTTCCCCTAC 
AGCTCTGGGG ATGCTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033314.2
CDS	139..3039
Translation	MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNDVWEIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYYDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHGKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVLCETMKDAPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAVPPLVNHTQDSANVTQPSLDSNKLLQDSSTPLPPSQPQEPVSGSCSQPSGDGPLQTTSPADGLSKNDNDLKVPVPLRKSRPLSMDARIQMDEEKQIPDQDENPSPAASKSQKANQSRPNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLSTSESMDYGTSLSADLSLNKETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVEKMEQDHSVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQKMEEHSQKKQRLDRDFVAKQKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRHDLLRKHEKEREQMQRYNQRMMEQLKVRQQQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIKQFSQQEEKRQKAERLQQQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLKEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS

Target ORF information:

RefSeq Version	NM_009288.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009288.2

ORF Insert Sequence:

ATGGCTTTCG CCAATTTCCG CCGCATCTTG CGGTTATCCA CCTTCGAGAA GAGAAAGTCC 
CGTGAATATG AGCACGTCCG CCGGGACCTG GACCCCAACG ATGTATGGGA GATCGTGGGC 
GAGCTGGGAG ACGGCGCCTT CGGCAAAGTC TACAAGGCCA AAAACAAGGA GACCGGTGCC 
CTGGCAGCAG CCAAAGTCAT AGAGACCAAG AGCGAGGAGG AGCTGGAGGA CTACATCGTG 
GAGATAGAGA TCCTGGCCAC CTGTGACCAC CCGTATATCG TGAAGCTCCT GGGGGCCTAT 
TACTATGATG GGAAGCTCTG GATCATGATT GAGTTCTGCC CTGGGGGAGC TGTGGATGCC 
ATCATGCTGG AGCTGGATCG AGGCCTCACT GAGCCCCAGA TTCAGGTCGT ATGCCGCCAG 
ATGTTGGAGG CTCTCAACTT CCTGCACGGC AAGAGGATCA TCCACCGAGA CCTGAAAGCT 
GGCAACGTGC TTATGACCCT TGAGGGGGAC ATCAGGCTGG CTGATTTTGG TGTGTCTGCC 
AAGAATCTGA AAACTCTGCA AAAGCGAGAC TCCTTCATAG GGACACCTTA CTGGATGGCC 
CCTGAGGTGG TGCTGTGTGA AACCATGAAG GACGCCCCCT ATGACTACAA GGCTGACATC 
TGGTCCTTGG GCATCACGCT GATCGAGATG GCGCAGATCG AGCCCCCACA CCACGAGCTC 
AACCCCATGC GGGTCCTGCT CAAGATTGCC AAGTCGGACC CTCCTACCCT GCTTACACCC 
TCCAAGTGGT CTGTGGAGTT CCGAGACTTT CTGAAGATAG CCTTGGATAA GAACCCAGAA 
ACCCGGCCCA GTGCTGCACA GCTGCTGCAG CATCCCTTCG TCAGCAGAGT CACCAGCAAT 
AAGGCTCTTC GGGAGCTGGT GGCCGAGGCA AAGGCGGAGG TAATGGAGGA GATTGAGGAT 
GGCAGGGAGG ACGGAGAAGA GGAGGATGCT GTGGATGCTG TTCCGCCCCT GGTCAACCAC 
ACTCAGGACT CTGCCAATGT AACTCAGCCA AGCCTCGACT CCAACAAGCT TCTCCAGGAT 
TCTTCCACCC CCCTGCCTCC CAGCCAGCCT CAGGAGCCTG TGAGCGGGTC CTGTAGCCAA 
CCCTCTGGGG ATGGACCCCT CCAAACCACC AGCCCTGCAG ATGGGCTCTC CAAGAATGAC 
AATGACTTAA AGGTGCCTGT TCCCCTCCGG AAGTCCCGGC CACTGTCCAT GGATGCCAGA 
ATTCAGATGG ATGAAGAGAA ACAAATCCCT GACCAGGATG AGAACCCGAG TCCTGCAGCC 
AGCAAGTCCC AAAAGGCCAA CCAGAGCCGA CCTAACAGCA GTGCCCTGGA GACCTTGGGT 
GGCGAGGCTC TGACCAACGG TGGCCTGGAA CTCCCCAGCT CTGTCACTCC AAGCCATTCT 
AAGAGGGCCT CGGACTGTAG CAACCTGTCT ACCTCAGAGA GCATGGACTA TGGCACCTCC 
CTGTCTGCTG ACCTGTCACT GAACAAAGAA ACGGGCTCAT TGTCTCTCAA GGGCTCAAAA 
CTGCACAATA AGACCCTGAA ACGGACCCGC CGGTTTGTGG TGGATGGTGT GGAGGTGAGC 
ATCACCACCT CCAAGATCAT CAGCGAGGAT GAGAAGAAAG ACGAGGAGAT GAGATTTCTC 
AGGCGCCAGG AGCTCCGAGA GCTTCGGCTT CTGCAGAAAG AAGAACATCG GAACCAGACG 
CAGCTGAGCA GCAAGCACGA GCTGCAGCTG GAGCAGATGC ACAAACGTTT TGAACAAGAA 
ATCAACGCCA AGAAGAAATT CTATGACGTG GAGCTAGAAA ACCTGGAGCG GCAGCAGAAG 
CAGCAGGTAG AGAAGATGGA GCAGGACCAC AGCGTGCGCC GCAAAGAGGA GGCCAAGCGG 
ATCCGCCTGG AGCAGGATCG AGACTACGCC AAGTTCCAAG AGCAACTCAA GCAAATGAAG 
AAGGAGGTGA AGAGTGAGGT TGAGAAACTG CCCCGGCAGC AGCGGAAGGA GAGCATGAAG 
CAGAAGATGG AGGAACACTC TCAGAAGAAA CAGCGGCTGG ACCGGGACTT TGTAGCCAAG 
CAGAAGGAGG ACCTGGAGCT GGCCATGAGG AAGCTCACCA CGGAAAACAG GCGTGAGATC 
TGCGACAAGG AACGAGATTG CCTTAGCAAG AAGCAGGAGC TCCTCCGAGA CCGTGAGGCG 
GCCCTGTGGG AGATGGAGGA GCACCAGTTG CAGGAGCGAC ATCAGCTGGT GAAGCAGCAA 
CTGAAGGACC AGTACTTCCT GCAGCGGCAT GACCTGCTGC GTAAGCATGA GAAGGAGCGG 
GAACAGATGC AGCGCTACAA CCAGCGCATG ATGGAGCAGC TGAAGGTCAG ACAGCAACAG 
GAGAAGGCGC GGCTACCCAA GATCCAGAGG AGCGACGGCA AGACGCGCAT GGCCATGTAC 
AAGAAGAGCC TGCACATCAA CGGTGCGGGC AGTGCCTCTG AGCAGCGCGA GAAGATCAAG 
CAGTTCTCCC AGCAGGAGGA GAAGAGGCAG AAGGCGGAGA GGCTGCAGCA GCAGCAGAAA 
CACGAGAACC AGATGCGAGA CATGGTGGCG CAGTGTGAAA GCAACATGAG CGAGCTACAG 
CAGCTGCAGA ATGAAAAGTG TCATCTGTTG GTAGAGCATG AAACCCAGAA GCTGAAGGCC 
CTGGATGAGA GCCACAACCA GAGCCTGAAG GAATGGCGAG ACAAGCTTCG GCCACGCAAA 
AAGGCCCTGG AAGAGGATTT GAACCAGAAG AAGCGGGAAC AGGAAATGTT CTTCAAATTA 
AGCGAGGAGG CAGAGCCCAG ACCCACCACA CCCAGCAAAG CCAGCAACTT CTTCCCCTAC 
AGCTCTGGGG ATGCTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stk10 ( NM_001359177.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346106.1 Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu77823
Clone ID Related Accession (Same CDS sequence)	NM_001359177.1 , XM_017314404.1
Accession Version	NM_001359177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase 10 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL669844.20 and BC096624.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCTG AGGTGGTGCT GTGTGAAACC ATGAAGGACG CCCCCTATGA CTACAAGGCT 
GACATCTGGT CCTTGGGCAT CACGCTGATC GAGATGGCGC AGATCGAGCC CCCACACCAC 
GAGCTCAACC CCATGCGGGT CCTGCTCAAG ATTGCCAAGT CGGACCCTCC TACCCTGCTT 
ACACCCTCCA AGTGGTCTGT GGAGTTCCGA GACTTTCTGA AGATAGCCTT GGATAAGAAC 
CCAGAAACCC GGCCCAGTGC TGCACAGCTG CTGCAGCATC CCTTCGTCAG CAGAGTCACC 
AGCAATAAGG CTCTTCGGGA GCTGGTGGCC GAGGCAAAGG CGGAGGTAAT GGAGGAGATT 
GAGGATGGCA GGGAGGACGG AGAAGAGGAG GATGCTGTGG ATGCTGTTCC GCCCCTGGTC 
AACCACACTC AGGACTCTGC CAATGTAACT CAGCCAAGCC TCGACTCCAA CAAGCTTCTC 
CAGGATTCTT CCACCCCCCT GCCTCCCAGC CAGCCTCAGG AGCCTGTGAG CGGGTCCTGT 
AGCCAACCCT CTGGGGATGG ACCCCTCCAA ACCACCAGCC CTGCAGATGG GCTCTCCAAG 
AATGACAATG ACTTAAAGGT GCCTGTTCCC CTCCGGAAGT CCCGGCCACT GTCCATGGAT 
GCCAGAATTC AGATGGATGA AGAGAAACAA ATCCCTGACC AGGATGAGAA CCCGAGTCCT 
GCAGCCAGCA AGTCCCAAAA GGCCAACCAG AGCCGACCTA ACAGCAGTGC CCTGGAGACC 
TTGGGTGGCG AGGCTCTGAC CAACGGTGGC CTGGAACTCC CCAGCTCTGT CACTCCAAGC 
CATTCTAAGA GGGCCTCGGA CTGTAGCAAC CTGTCTACCT CAGAGAGCAT GGACTATGGC 
ACCTCCCTGT CTGCTGACCT GTCACTGAAC AAAGAAACGG GCTCATTGTC TCTCAAGGGC 
TCAAAACTGC ACAATAAGAC CCTGAAACGG ACCCGCCGGT TTGTGGTGGA TGGTGTGGAG 
GTGAGCATCA CCACCTCCAA GATCATCAGC GAGGATGAGA AGAAAGACGA GGAGATGAGA 
TTTCTCAGGC GCCAGGAGCT CCGAGAGCTT CGGCTTCTGC AGAAAGAAGA ACATCGGAAC 
CAGACGCAGC TGAGCAGCAA GCACGAGCTG CAGCTGGAGC AGATGCACAA ACGTTTTGAA 
CAAGAAATCA ACGCCAAGAA GAAATTCTAT GACGTGGAGC TAGAAAACCT GGAGCGGCAG 
CAGAAGCAGC AGGTAGAGAA GATGGAGCAG GACCACAGCG TGCGCCGCAA AGAGGAGGCC 
AAGCGGATCC GCCTGGAGCA GGATCGAGAC TACGCCAAGT TCCAAGAGCA ACTCAAGCAA 
ATGAAGAAGG AGGTGAAGAG TGAGGTTGAG AAACTGCCCC GGCAGCAGCG GAAGGAGAGC 
ATGAAGCAGA AGATGGAGGA ACACTCTCAG AAGAAACAGC GGCTGGACCG GGACTTTGTA 
GCCAAGCAGA AGGAGGACCT GGAGCTGGCC ATGAGGAAGC TCACCACGGA AAACAGGCGT 
GAGATCTGCG ACAAGGAACG AGATTGCCTT AGCAAGAAGC AGGAGCTCCT CCGAGACCGT 
GAGGCGGCCC TGTGGGAGAT GGAGGAGCAC CAGTTGCAGG AGCGACATCA GCTGGTGAAG 
CAGCAACTGA AGGACCAGTA CTTCCTGCAG CGGCATGACC TGCTGCGTAA GCATGAGAAG 
GAGCGGGAAC AGATGCAGCG CTACAACCAG CGCATGATGG AGCAGCTGAA GGTCAGACAG 
CAACAGGAGA AGGCGCGGCT ACCCAAGATC CAGAGGAGCG ACGGCAAGAC GCGCATGGCC 
ATGTACAAGA AGAGCCTGCA CATCAACGGT GCGGGCAGTG CCTCTGAGCA GCGCGAGAAG 
ATCAAGCAGT TCTCCCAGCA GGAGGAGAAG AGGCAGAAGG CGGAGAGGCT GCAGCAGCAG 
CAGAAACACG AGAACCAGAT GCGAGACATG GTGGCGCAGT GTGAAAGCAA CATGAGCGAG 
CTACAGCAGC TGCAGAATGA AAAGTGTCAT CTGTTGGTAG AGCATGAAAC CCAGAAGCTG 
AAGGCCCTGG ATGAGAGCCA CAACCAGAGC CTGAAGGAAT GGCGAGACAA GCTTCGGCCA 
CGCAAAAAGG CCCTGGAAGA GGATTTGAAC CAGAAGAAGC GGGAACAGGA AATGTTCTTC 
AAATTAAGCG AGGAGGCAGA GCCCAGACCC ACCACACCCA GCAAAGCCAG CAACTTCTTC 
CCCTACAGCT CTGGGGATGC TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346106.1
CDS	953..3259
Translation	MAPEVVLCETMKDAPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAVPPLVNHTQDSANVTQPSLDSNKLLQDSSTPLPPSQPQEPVSGSCSQPSGDGPLQTTSPADGLSKNDNDLKVPVPLRKSRPLSMDARIQMDEEKQIPDQDENPSPAASKSQKANQSRPNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLSTSESMDYGTSLSADLSLNKETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVEKMEQDHSVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQKMEEHSQKKQRLDRDFVAKQKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRHDLLRKHEKEREQMQRYNQRMMEQLKVRQQQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIKQFSQQEEKRQKAERLQQQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLKEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS

Target ORF information:

RefSeq Version	NM_001359177.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359177.1

ORF Insert Sequence:

ATGGCCCCTG AGGTGGTGCT GTGTGAAACC ATGAAGGACG CCCCCTATGA CTACAAGGCT 
GACATCTGGT CCTTGGGCAT CACGCTGATC GAGATGGCGC AGATCGAGCC CCCACACCAC 
GAGCTCAACC CCATGCGGGT CCTGCTCAAG ATTGCCAAGT CGGACCCTCC TACCCTGCTT 
ACACCCTCCA AGTGGTCTGT GGAGTTCCGA GACTTTCTGA AGATAGCCTT GGATAAGAAC 
CCAGAAACCC GGCCCAGTGC TGCACAGCTG CTGCAGCATC CCTTCGTCAG CAGAGTCACC 
AGCAATAAGG CTCTTCGGGA GCTGGTGGCC GAGGCAAAGG CGGAGGTAAT GGAGGAGATT 
GAGGATGGCA GGGAGGACGG AGAAGAGGAG GATGCTGTGG ATGCTGTTCC GCCCCTGGTC 
AACCACACTC AGGACTCTGC CAATGTAACT CAGCCAAGCC TCGACTCCAA CAAGCTTCTC 
CAGGATTCTT CCACCCCCCT GCCTCCCAGC CAGCCTCAGG AGCCTGTGAG CGGGTCCTGT 
AGCCAACCCT CTGGGGATGG ACCCCTCCAA ACCACCAGCC CTGCAGATGG GCTCTCCAAG 
AATGACAATG ACTTAAAGGT GCCTGTTCCC CTCCGGAAGT CCCGGCCACT GTCCATGGAT 
GCCAGAATTC AGATGGATGA AGAGAAACAA ATCCCTGACC AGGATGAGAA CCCGAGTCCT 
GCAGCCAGCA AGTCCCAAAA GGCCAACCAG AGCCGACCTA ACAGCAGTGC CCTGGAGACC 
TTGGGTGGCG AGGCTCTGAC CAACGGTGGC CTGGAACTCC CCAGCTCTGT CACTCCAAGC 
CATTCTAAGA GGGCCTCGGA CTGTAGCAAC CTGTCTACCT CAGAGAGCAT GGACTATGGC 
ACCTCCCTGT CTGCTGACCT GTCACTGAAC AAAGAAACGG GCTCATTGTC TCTCAAGGGC 
TCAAAACTGC ACAATAAGAC CCTGAAACGG ACCCGCCGGT TTGTGGTGGA TGGTGTGGAG 
GTGAGCATCA CCACCTCCAA GATCATCAGC GAGGATGAGA AGAAAGACGA GGAGATGAGA 
TTTCTCAGGC GCCAGGAGCT CCGAGAGCTT CGGCTTCTGC AGAAAGAAGA ACATCGGAAC 
CAGACGCAGC TGAGCAGCAA GCACGAGCTG CAGCTGGAGC AGATGCACAA ACGTTTTGAA 
CAAGAAATCA ACGCCAAGAA GAAATTCTAT GACGTGGAGC TAGAAAACCT GGAGCGGCAG 
CAGAAGCAGC AGGTAGAGAA GATGGAGCAG GACCACAGCG TGCGCCGCAA AGAGGAGGCC 
AAGCGGATCC GCCTGGAGCA GGATCGAGAC TACGCCAAGT TCCAAGAGCA ACTCAAGCAA 
ATGAAGAAGG AGGTGAAGAG TGAGGTTGAG AAACTGCCCC GGCAGCAGCG GAAGGAGAGC 
ATGAAGCAGA AGATGGAGGA ACACTCTCAG AAGAAACAGC GGCTGGACCG GGACTTTGTA 
GCCAAGCAGA AGGAGGACCT GGAGCTGGCC ATGAGGAAGC TCACCACGGA AAACAGGCGT 
GAGATCTGCG ACAAGGAACG AGATTGCCTT AGCAAGAAGC AGGAGCTCCT CCGAGACCGT 
GAGGCGGCCC TGTGGGAGAT GGAGGAGCAC CAGTTGCAGG AGCGACATCA GCTGGTGAAG 
CAGCAACTGA AGGACCAGTA CTTCCTGCAG CGGCATGACC TGCTGCGTAA GCATGAGAAG 
GAGCGGGAAC AGATGCAGCG CTACAACCAG CGCATGATGG AGCAGCTGAA GGTCAGACAG 
CAACAGGAGA AGGCGCGGCT ACCCAAGATC CAGAGGAGCG ACGGCAAGAC GCGCATGGCC 
ATGTACAAGA AGAGCCTGCA CATCAACGGT GCGGGCAGTG CCTCTGAGCA GCGCGAGAAG 
ATCAAGCAGT TCTCCCAGCA GGAGGAGAAG AGGCAGAAGG CGGAGAGGCT GCAGCAGCAG 
CAGAAACACG AGAACCAGAT GCGAGACATG GTGGCGCAGT GTGAAAGCAA CATGAGCGAG 
CTACAGCAGC TGCAGAATGA AAAGTGTCAT CTGTTGGTAG AGCATGAAAC CCAGAAGCTG 
AAGGCCCTGG ATGAGAGCCA CAACCAGAGC CTGAAGGAAT GGCGAGACAA GCTTCGGCCA 
CGCAAAAAGG CCCTGGAAGA GGATTTGAAC CAGAAGAAGC GGGAACAGGA AATGTTCTTC 
AAATTAAGCG AGGAGGCAGA GCCCAGACCC ACCACACCCA GCAAAGCCAG CAACTTCTTC 
CCCTACAGCT CTGGGGATGC TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stk10 ( NM_001359178.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346107.1 Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 3, mRNA.

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CloneID	OMu83659
Clone ID Related Accession (Same CDS sequence)	NM_001359178.1
Accession Version	NM_001359178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase 10 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY207613.1, BC096624.1, AL669844.20 and AK148118.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which has a shorter and distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATAAGAA GGCTGTCCCA TGGACCCTGT CTCCACACAC CCAGCCCTGG GCGTGCGGTG 
CCCGGGCTGT GGCTCACTCC TTACTTACGC CTGTCCTCCA GGTCTGTGGA GTTCCGAGAC 
TTTCTGAAGA TAGCCTTGGA TAAGAACCCA GAAACCCGGC CCAGTGCTGC ACAGCTGCTG 
CAGCATCCCT TCGTCAGCAG AGTCACCAGC AATAAGGCTC TTCGGGAGCT GGTGGCCGAG 
GCAAAGGCGG AGGTAATGGA GGAGATTGAG GATGGCAGGG AGGACGGAGA AGAGGAGGAT 
GCTGTGGATG CTGTTCCGCC CCTGGTCAAC CACACTCAGG ACTCTGCCAA TGTAACTCAG 
CCAAGCCTCG ACTCCAACAA GCTTCTCCAG GATTCTTCCA CCCCCCTGCC TCCCAGCCAG 
CCTCAGGAGC CTGTGAGCGG GTCCTGTAGC CAACCCTCTG GGGATGGACC CCTCCAAACC 
ACCAGCCCTG CAGATGGGCT CTCCAAGAAT GACAATGACT TAAAGGTGCC TGTTCCCCTC 
CGGAAGTCCC GGCCACTGTC CATGGATGCC AGAATTCAGA TGGATGAAGA GAAACAAATC 
CCTGACCAGG ATGAGAACCC GAGTCCTGCA GCCAGCAAGT CCCAAAAGGC CAACCAGAGC 
CGACCTAACA GCAGTGCCCT GGAGACCTTG GGTGGCGAGG CTCTGACCAA CGGTGGCCTG 
GAACTCCCCA GCTCTGTCAC TCCAAGCCAT TCTAAGAGGG CCTCGGACTG TAGCAACCTG 
TCTACCTCAG AGAGCATGGA CTATGGCACC TCCCTGTCTG CTGACCTGTC ACTGAACAAA 
GAAACGGGCT CATTGTCTCT CAAGGGCTCA AAACTGCACA ATAAGACCCT GAAACGGACC 
CGCCGGTTTG TGGTGGATGG TGTGGAGGTG AGCATCACCA CCTCCAAGAT CATCAGCGAG 
GATGAGAAGA AAGACGAGGA GATGAGATTT CTCAGGCGCC AGGAGCTCCG AGAGCTTCGG 
CTTCTGCAGA AAGAAGAACA TCGGAACCAG ACGCAGCTGA GCAGCAAGCA CGAGCTGCAG 
CTGGAGCAGA TGCACAAACG TTTTGAACAA GAAATCAACG CCAAGAAGAA ATTCTATGAC 
GTGGAGCTAG AAAACCTGGA GCGGCAGCAG AAGCAGCAGG TAGAGAAGAT GGAGCAGGAC 
CACAGCGTGC GCCGCAAAGA GGAGGCCAAG CGGATCCGCC TGGAGCAGGA TCGAGACTAC 
GCCAAGTTCC AAGAGCAACT CAAGCAAATG AAGAAGGAGG TGAAGAGTGA GGTTGAGAAA 
CTGCCCCGGC AGCAGCGGAA GGAGAGCATG AAGCAGAAGA TGGAGGAACA CTCTCAGAAG 
AAACAGCGGC TGGACCGGGA CTTTGTAGCC AAGCAGAAGG AGGACCTGGA GCTGGCCATG 
AGGAAGCTCA CCACGGAAAA CAGGCGTGAG ATCTGCGACA AGGAACGAGA TTGCCTTAGC 
AAGAAGCAGG AGCTCCTCCG AGACCGTGAG GCGGCCCTGT GGGAGATGGA GGAGCACCAG 
TTGCAGGAGC GACATCAGCT GGTGAAGCAG CAACTGAAGG ACCAGTACTT CCTGCAGCGG 
CATGACCTGC TGCGTAAGCA TGAGAAGGAG CGGGAACAGA TGCAGCGCTA CAACCAGCGC 
ATGATGGAGC AGCTGAAGGT CAGACAGCAA CAGGAGAAGG CGCGGCTACC CAAGATCCAG 
AGGAGCGACG GCAAGACGCG CATGGCCATG TACAAGAAGA GCCTGCACAT CAACGGTGCG 
GGCAGTGCCT CTGAGCAGCG CGAGAAGATC AAGCAGTTCT CCCAGCAGGA GGAGAAGAGG 
CAGAAGGCGG AGAGGCTGCA GCAGCAGCAG AAACACGAGA ACCAGATGCG AGACATGGTG 
GCGCAGTGTG AAAGCAACAT GAGCGAGCTA CAGCAGCTGC AGAATGAAAA GTGTCATCTG 
TTGGTAGAGC ATGAAACCCA GAAGCTGAAG GCCCTGGATG AGAGCCACAA CCAGAGCCTG 
AAGGAATGGC GAGACAAGCT TCGGCCACGC AAAAAGGCCC TGGAAGAGGA TTTGAACCAG 
AAGAAGCGGG AACAGGAAAT GTTCTTCAAA TTAAGCGAGG AGGCAGAGCC CAGACCCACC 
ACACCCAGCA AAGCCAGCAA CTTCTTCCCC TACAGCTCTG GGGATGCTTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346107.1
CDS	954..3167
Translation	MIRRLSHGPCLHTPSPGRAVPGLWLTPYLRLSSRSVEFRDFLKIALDKNPETRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAVPPLVNHTQDSANVTQPSLDSNKLLQDSSTPLPPSQPQEPVSGSCSQPSGDGPLQTTSPADGLSKNDNDLKVPVPLRKSRPLSMDARIQMDEEKQIPDQDENPSPAASKSQKANQSRPNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLSTSESMDYGTSLSADLSLNKETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVEKMEQDHSVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQKMEEHSQKKQRLDRDFVAKQKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRHDLLRKHEKEREQMQRYNQRMMEQLKVRQQQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIKQFSQQEEKRQKAERLQQQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLKEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS

Target ORF information:

RefSeq Version	NM_001359178.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359178.1

ORF Insert Sequence:

ATGATAAGAA GGCTGTCCCA TGGACCCTGT CTCCACACAC CCAGCCCTGG GCGTGCGGTG 
CCCGGGCTGT GGCTCACTCC TTACTTACGC CTGTCCTCCA GGTCTGTGGA GTTCCGAGAC 
TTTCTGAAGA TAGCCTTGGA TAAGAACCCA GAAACCCGGC CCAGTGCTGC ACAGCTGCTG 
CAGCATCCCT TCGTCAGCAG AGTCACCAGC AATAAGGCTC TTCGGGAGCT GGTGGCCGAG 
GCAAAGGCGG AGGTAATGGA GGAGATTGAG GATGGCAGGG AGGACGGAGA AGAGGAGGAT 
GCTGTGGATG CTGTTCCGCC CCTGGTCAAC CACACTCAGG ACTCTGCCAA TGTAACTCAG 
CCAAGCCTCG ACTCCAACAA GCTTCTCCAG GATTCTTCCA CCCCCCTGCC TCCCAGCCAG 
CCTCAGGAGC CTGTGAGCGG GTCCTGTAGC CAACCCTCTG GGGATGGACC CCTCCAAACC 
ACCAGCCCTG CAGATGGGCT CTCCAAGAAT GACAATGACT TAAAGGTGCC TGTTCCCCTC 
CGGAAGTCCC GGCCACTGTC CATGGATGCC AGAATTCAGA TGGATGAAGA GAAACAAATC 
CCTGACCAGG ATGAGAACCC GAGTCCTGCA GCCAGCAAGT CCCAAAAGGC CAACCAGAGC 
CGACCTAACA GCAGTGCCCT GGAGACCTTG GGTGGCGAGG CTCTGACCAA CGGTGGCCTG 
GAACTCCCCA GCTCTGTCAC TCCAAGCCAT TCTAAGAGGG CCTCGGACTG TAGCAACCTG 
TCTACCTCAG AGAGCATGGA CTATGGCACC TCCCTGTCTG CTGACCTGTC ACTGAACAAA 
GAAACGGGCT CATTGTCTCT CAAGGGCTCA AAACTGCACA ATAAGACCCT GAAACGGACC 
CGCCGGTTTG TGGTGGATGG TGTGGAGGTG AGCATCACCA CCTCCAAGAT CATCAGCGAG 
GATGAGAAGA AAGACGAGGA GATGAGATTT CTCAGGCGCC AGGAGCTCCG AGAGCTTCGG 
CTTCTGCAGA AAGAAGAACA TCGGAACCAG ACGCAGCTGA GCAGCAAGCA CGAGCTGCAG 
CTGGAGCAGA TGCACAAACG TTTTGAACAA GAAATCAACG CCAAGAAGAA ATTCTATGAC 
GTGGAGCTAG AAAACCTGGA GCGGCAGCAG AAGCAGCAGG TAGAGAAGAT GGAGCAGGAC 
CACAGCGTGC GCCGCAAAGA GGAGGCCAAG CGGATCCGCC TGGAGCAGGA TCGAGACTAC 
GCCAAGTTCC AAGAGCAACT CAAGCAAATG AAGAAGGAGG TGAAGAGTGA GGTTGAGAAA 
CTGCCCCGGC AGCAGCGGAA GGAGAGCATG AAGCAGAAGA TGGAGGAACA CTCTCAGAAG 
AAACAGCGGC TGGACCGGGA CTTTGTAGCC AAGCAGAAGG AGGACCTGGA GCTGGCCATG 
AGGAAGCTCA CCACGGAAAA CAGGCGTGAG ATCTGCGACA AGGAACGAGA TTGCCTTAGC 
AAGAAGCAGG AGCTCCTCCG AGACCGTGAG GCGGCCCTGT GGGAGATGGA GGAGCACCAG 
TTGCAGGAGC GACATCAGCT GGTGAAGCAG CAACTGAAGG ACCAGTACTT CCTGCAGCGG 
CATGACCTGC TGCGTAAGCA TGAGAAGGAG CGGGAACAGA TGCAGCGCTA CAACCAGCGC 
ATGATGGAGC AGCTGAAGGT CAGACAGCAA CAGGAGAAGG CGCGGCTACC CAAGATCCAG 
AGGAGCGACG GCAAGACGCG CATGGCCATG TACAAGAAGA GCCTGCACAT CAACGGTGCG 
GGCAGTGCCT CTGAGCAGCG CGAGAAGATC AAGCAGTTCT CCCAGCAGGA GGAGAAGAGG 
CAGAAGGCGG AGAGGCTGCA GCAGCAGCAG AAACACGAGA ACCAGATGCG AGACATGGTG 
GCGCAGTGTG AAAGCAACAT GAGCGAGCTA CAGCAGCTGC AGAATGAAAA GTGTCATCTG 
TTGGTAGAGC ATGAAACCCA GAAGCTGAAG GCCCTGGATG AGAGCCACAA CCAGAGCCTG 
AAGGAATGGC GAGACAAGCT TCGGCCACGC AAAAAGGCCC TGGAAGAGGA TTTGAACCAG 
AAGAAGCGGG AACAGGAAAT GTTCTTCAAA TTAAGCGAGG AGGCAGAGCC CAGACCCACC 
ACACCCAGCA AAGCCAGCAA CTTCTTCCCC TACAGCTCTG GGGATGCTTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stk10 ( XM_017314404.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169893.1 Mus musculus serine/threonine kinase 10 (Stk10), transcript variant X1, mRNA.

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CloneID	OMu77823
Clone ID Related Accession (Same CDS sequence)	NM_001359177.1 , XM_017314404.1
Accession Version	XM_017314404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG AGGTGGTGCT GTGTGAAACC ATGAAGGACG CCCCCTATGA CTACAAGGCT 
GACATCTGGT CCTTGGGCAT CACGCTGATC GAGATGGCGC AGATCGAGCC CCCACACCAC 
GAGCTCAACC CCATGCGGGT CCTGCTCAAG ATTGCCAAGT CGGACCCTCC TACCCTGCTT 
ACACCCTCCA AGTGGTCTGT GGAGTTCCGA GACTTTCTGA AGATAGCCTT GGATAAGAAC 
CCAGAAACCC GGCCCAGTGC TGCACAGCTG CTGCAGCATC CCTTCGTCAG CAGAGTCACC 
AGCAATAAGG CTCTTCGGGA GCTGGTGGCC GAGGCAAAGG CGGAGGTAAT GGAGGAGATT 
GAGGATGGCA GGGAGGACGG AGAAGAGGAG GATGCTGTGG ATGCTGTTCC GCCCCTGGTC 
AACCACACTC AGGACTCTGC CAATGTAACT CAGCCAAGCC TCGACTCCAA CAAGCTTCTC 
CAGGATTCTT CCACCCCCCT GCCTCCCAGC CAGCCTCAGG AGCCTGTGAG CGGGTCCTGT 
AGCCAACCCT CTGGGGATGG ACCCCTCCAA ACCACCAGCC CTGCAGATGG GCTCTCCAAG 
AATGACAATG ACTTAAAGGT GCCTGTTCCC CTCCGGAAGT CCCGGCCACT GTCCATGGAT 
GCCAGAATTC AGATGGATGA AGAGAAACAA ATCCCTGACC AGGATGAGAA CCCGAGTCCT 
GCAGCCAGCA AGTCCCAAAA GGCCAACCAG AGCCGACCTA ACAGCAGTGC CCTGGAGACC 
TTGGGTGGCG AGGCTCTGAC CAACGGTGGC CTGGAACTCC CCAGCTCTGT CACTCCAAGC 
CATTCTAAGA GGGCCTCGGA CTGTAGCAAC CTGTCTACCT CAGAGAGCAT GGACTATGGC 
ACCTCCCTGT CTGCTGACCT GTCACTGAAC AAAGAAACGG GCTCATTGTC TCTCAAGGGC 
TCAAAACTGC ACAATAAGAC CCTGAAACGG ACCCGCCGGT TTGTGGTGGA TGGTGTGGAG 
GTGAGCATCA CCACCTCCAA GATCATCAGC GAGGATGAGA AGAAAGACGA GGAGATGAGA 
TTTCTCAGGC GCCAGGAGCT CCGAGAGCTT CGGCTTCTGC AGAAAGAAGA ACATCGGAAC 
CAGACGCAGC TGAGCAGCAA GCACGAGCTG CAGCTGGAGC AGATGCACAA ACGTTTTGAA 
CAAGAAATCA ACGCCAAGAA GAAATTCTAT GACGTGGAGC TAGAAAACCT GGAGCGGCAG 
CAGAAGCAGC AGGTAGAGAA GATGGAGCAG GACCACAGCG TGCGCCGCAA AGAGGAGGCC 
AAGCGGATCC GCCTGGAGCA GGATCGAGAC TACGCCAAGT TCCAAGAGCA ACTCAAGCAA 
ATGAAGAAGG AGGTGAAGAG TGAGGTTGAG AAACTGCCCC GGCAGCAGCG GAAGGAGAGC 
ATGAAGCAGA AGATGGAGGA ACACTCTCAG AAGAAACAGC GGCTGGACCG GGACTTTGTA 
GCCAAGCAGA AGGAGGACCT GGAGCTGGCC ATGAGGAAGC TCACCACGGA AAACAGGCGT 
GAGATCTGCG ACAAGGAACG AGATTGCCTT AGCAAGAAGC AGGAGCTCCT CCGAGACCGT 
GAGGCGGCCC TGTGGGAGAT GGAGGAGCAC CAGTTGCAGG AGCGACATCA GCTGGTGAAG 
CAGCAACTGA AGGACCAGTA CTTCCTGCAG CGGCATGACC TGCTGCGTAA GCATGAGAAG 
GAGCGGGAAC AGATGCAGCG CTACAACCAG CGCATGATGG AGCAGCTGAA GGTCAGACAG 
CAACAGGAGA AGGCGCGGCT ACCCAAGATC CAGAGGAGCG ACGGCAAGAC GCGCATGGCC 
ATGTACAAGA AGAGCCTGCA CATCAACGGT GCGGGCAGTG CCTCTGAGCA GCGCGAGAAG 
ATCAAGCAGT TCTCCCAGCA GGAGGAGAAG AGGCAGAAGG CGGAGAGGCT GCAGCAGCAG 
CAGAAACACG AGAACCAGAT GCGAGACATG GTGGCGCAGT GTGAAAGCAA CATGAGCGAG 
CTACAGCAGC TGCAGAATGA AAAGTGTCAT CTGTTGGTAG AGCATGAAAC CCAGAAGCTG 
AAGGCCCTGG ATGAGAGCCA CAACCAGAGC CTGAAGGAAT GGCGAGACAA GCTTCGGCCA 
CGCAAAAAGG CCCTGGAAGA GGATTTGAAC CAGAAGAAGC GGGAACAGGA AATGTTCTTC 
AAATTAAGCG AGGAGGCAGA GCCCAGACCC ACCACACCCA GCAAAGCCAG CAACTTCTTC 
CCCTACAGCT CTGGGGATGC TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169893.1
CDS	898..3204
Translation	MAPEVVLCETMKDAPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAVPPLVNHTQDSANVTQPSLDSNKLLQDSSTPLPPSQPQEPVSGSCSQPSGDGPLQTTSPADGLSKNDNDLKVPVPLRKSRPLSMDARIQMDEEKQIPDQDENPSPAASKSQKANQSRPNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLSTSESMDYGTSLSADLSLNKETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVEKMEQDHSVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQKMEEHSQKKQRLDRDFVAKQKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRHDLLRKHEKEREQMQRYNQRMMEQLKVRQQQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIKQFSQQEEKRQKAERLQQQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLKEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS

Target ORF information:

RefSeq Version	XM_017314404.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine/threonine kinase 10 (Stk10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314404.1

ORF Insert Sequence:

ATGGCCCCTG AGGTGGTGCT GTGTGAAACC ATGAAGGACG CCCCCTATGA CTACAAGGCT 
GACATCTGGT CCTTGGGCAT CACGCTGATC GAGATGGCGC AGATCGAGCC CCCACACCAC 
GAGCTCAACC CCATGCGGGT CCTGCTCAAG ATTGCCAAGT CGGACCCTCC TACCCTGCTT 
ACACCCTCCA AGTGGTCTGT GGAGTTCCGA GACTTTCTGA AGATAGCCTT GGATAAGAAC 
CCAGAAACCC GGCCCAGTGC TGCACAGCTG CTGCAGCATC CCTTCGTCAG CAGAGTCACC 
AGCAATAAGG CTCTTCGGGA GCTGGTGGCC GAGGCAAAGG CGGAGGTAAT GGAGGAGATT 
GAGGATGGCA GGGAGGACGG AGAAGAGGAG GATGCTGTGG ATGCTGTTCC GCCCCTGGTC 
AACCACACTC AGGACTCTGC CAATGTAACT CAGCCAAGCC TCGACTCCAA CAAGCTTCTC 
CAGGATTCTT CCACCCCCCT GCCTCCCAGC CAGCCTCAGG AGCCTGTGAG CGGGTCCTGT 
AGCCAACCCT CTGGGGATGG ACCCCTCCAA ACCACCAGCC CTGCAGATGG GCTCTCCAAG 
AATGACAATG ACTTAAAGGT GCCTGTTCCC CTCCGGAAGT CCCGGCCACT GTCCATGGAT 
GCCAGAATTC AGATGGATGA AGAGAAACAA ATCCCTGACC AGGATGAGAA CCCGAGTCCT 
GCAGCCAGCA AGTCCCAAAA GGCCAACCAG AGCCGACCTA ACAGCAGTGC CCTGGAGACC 
TTGGGTGGCG AGGCTCTGAC CAACGGTGGC CTGGAACTCC CCAGCTCTGT CACTCCAAGC 
CATTCTAAGA GGGCCTCGGA CTGTAGCAAC CTGTCTACCT CAGAGAGCAT GGACTATGGC 
ACCTCCCTGT CTGCTGACCT GTCACTGAAC AAAGAAACGG GCTCATTGTC TCTCAAGGGC 
TCAAAACTGC ACAATAAGAC CCTGAAACGG ACCCGCCGGT TTGTGGTGGA TGGTGTGGAG 
GTGAGCATCA CCACCTCCAA GATCATCAGC GAGGATGAGA AGAAAGACGA GGAGATGAGA 
TTTCTCAGGC GCCAGGAGCT CCGAGAGCTT CGGCTTCTGC AGAAAGAAGA ACATCGGAAC 
CAGACGCAGC TGAGCAGCAA GCACGAGCTG CAGCTGGAGC AGATGCACAA ACGTTTTGAA 
CAAGAAATCA ACGCCAAGAA GAAATTCTAT GACGTGGAGC TAGAAAACCT GGAGCGGCAG 
CAGAAGCAGC AGGTAGAGAA GATGGAGCAG GACCACAGCG TGCGCCGCAA AGAGGAGGCC 
AAGCGGATCC GCCTGGAGCA GGATCGAGAC TACGCCAAGT TCCAAGAGCA ACTCAAGCAA 
ATGAAGAAGG AGGTGAAGAG TGAGGTTGAG AAACTGCCCC GGCAGCAGCG GAAGGAGAGC 
ATGAAGCAGA AGATGGAGGA ACACTCTCAG AAGAAACAGC GGCTGGACCG GGACTTTGTA 
GCCAAGCAGA AGGAGGACCT GGAGCTGGCC ATGAGGAAGC TCACCACGGA AAACAGGCGT 
GAGATCTGCG ACAAGGAACG AGATTGCCTT AGCAAGAAGC AGGAGCTCCT CCGAGACCGT 
GAGGCGGCCC TGTGGGAGAT GGAGGAGCAC CAGTTGCAGG AGCGACATCA GCTGGTGAAG 
CAGCAACTGA AGGACCAGTA CTTCCTGCAG CGGCATGACC TGCTGCGTAA GCATGAGAAG 
GAGCGGGAAC AGATGCAGCG CTACAACCAG CGCATGATGG AGCAGCTGAA GGTCAGACAG 
CAACAGGAGA AGGCGCGGCT ACCCAAGATC CAGAGGAGCG ACGGCAAGAC GCGCATGGCC 
ATGTACAAGA AGAGCCTGCA CATCAACGGT GCGGGCAGTG CCTCTGAGCA GCGCGAGAAG 
ATCAAGCAGT TCTCCCAGCA GGAGGAGAAG AGGCAGAAGG CGGAGAGGCT GCAGCAGCAG 
CAGAAACACG AGAACCAGAT GCGAGACATG GTGGCGCAGT GTGAAAGCAA CATGAGCGAG 
CTACAGCAGC TGCAGAATGA AAAGTGTCAT CTGTTGGTAG AGCATGAAAC CCAGAAGCTG 
AAGGCCCTGG ATGAGAGCCA CAACCAGAGC CTGAAGGAAT GGCGAGACAA GCTTCGGCCA 
CGCAAAAAGG CCCTGGAAGA GGATTTGAAC CAGAAGAAGC GGGAACAGGA AATGTTCTTC 
AAATTAAGCG AGGAGGCAGA GCCCAGACCC ACCACACCCA GCAAAGCCAG CAACTTCTTC 
CCCTACAGCT CTGGGGATGC TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal DNA Methylation Regulates Early Trophoblast Development.
Developmental cell36(2)152-63(2016 Jan)
Branco MR,King M,Perez-Garcia V,Bogutz AB,Caley M,Fineberg E,Lefebvre L,Cook SJ,Dean W,Hemberger M,Reik W

A combination of SILAC and nucleotide acyl phosphate labelling reveals unexpected targets of the Rsk inhibitor BI-D1870.
Bioscience reports34(1)(2014 Feb)
Edgar AJ,Trost M,Watts C,Zaru R

LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation.
Proceedings of the National Academy of Sciences of the United States of America106(12)4707-12(2009 Mar)
Belkina NV,Liu Y,Hao JJ,Karasuyama H,Shaw S

Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.
The EMBO journal27(4)704-14(2008 Feb)
Pike AC,Rellos P,Niesen FH,Turnbull A,Oliver AW,Parker SA,Turk BE,Pearl LH,Knapp S

Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue.
The Journal of biological chemistry278(20)18221-8(2003 May)
Walter SA,Cutler RE Jr,Martinez R,Gishizky M,Hill RJ

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Stk10 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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