gRNAs
The following Shroom4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shroom4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu14938 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001040459.2 | |
| Accession Version | NM_001040459.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-19 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein Shroom4 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151022.1 and AL671501.8. On Sep 1, 2015 this sequence version replaced NM_001040459.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC151022.1, DQ435686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGAGCC GGCCGGGGTC CTTCCAGTAC GTCCCGGTGC AGCTGCAAGG GGGGGCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001035549.1 |
| CDS | 114..4541 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001040459.2 |
1 | ATGGAGAGCC GGCCGGGGTC CTTCCAGTAC GTCCCGGTGC AGCTGCAAGG GGGGGCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu55868 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006527549.4 , XM_006527549.3 , NM_001313764.1 | |
| Accession Version | NM_001313764.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-19 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein Shroom4 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101431.1, BC151022.1 and AL671501.8. Transcript Variant: This variant (2) uses an alternate exon in place of the first exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTATGG CTCCCGCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001300693.1 |
| CDS | 156..4430 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001313764.1 |
1 | ATGCGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTATGG CTCCCGCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu55868 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006527549.4 , XM_006527549.3 , NM_001313764.1 | |
| Accession Version | XM_006527549.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein Shroom4 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTATGG CTCCCGCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006527612.1 |
| CDS | 268..4542 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006527549.3 |
1 | ATGCGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTATGG CTCCCGCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu83527 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001313765.1 | |
| Accession Version | NM_001313765.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein Shroom4 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052531.1, AK173129.1 and AL671501.8. Transcript Variant: This variant (3) uses an alternate exon in place of the first two exons compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK173129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGTTGCAA CACTAGTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001300694.1 |
| CDS | 391..4470 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001313765.1 |
1 | ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGTTGCAA CACTAGTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu55868 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006527549.4 , XM_006527549.3 , NM_001313764.1 | |
| Accession Version | XM_006527549.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein Shroom4 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527549.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTATGG CTCCCGCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006527612.1 |
| CDS | 259..4533 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006527549.4 |
1 | ATGCGGACTG GTGATGAGCT GGTGAATATC AATGGCACTC CATTGTATGG CTCCCGCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions. |
Shroom4 (Kiaa1202) is an actin-associated protein implicated in cytoskeletal organization. |
Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins. |
A new standard nomenclature for proteins related to Apx and Shroom. |
Disruptions of the novel KIAA1202 gene are associated with X-linked mental retardation. |
