Slfn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slfn2
Entrez Gene ID	20556
Full Name	schlafen 2
Synonyms	Shlf2
General protein information	Preferred Names schlafen 2 Names schlafen family member 12-like OTTMUSCASG00059410 OTTMUSPWKG00059397 OTTMUSWSBG00059303 Schlafen family member 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 C\|11 50.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011408.1	NP_035538.1	schlafen family member 12-like

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slfn2	NP_035538.1
Rattus norvegicus (Norway rat)	Slfn2	NP_001100501.1

Gene Ontology
Bibliography

Related articles in PubMed

Slfn2 mutation-induced loss of T-cell quiescence leads to elevated de novo sterol synthesis.
Omar I, Rom O, Aviram M, Cohen-Daniel L, Gebre AK, Parks JS, Berger M
Immunology152(3)484-493(2017 Nov)

Schlafen2 mutation unravels a role for chronic ER stress in the loss of T cell quiescence.
Omar I, Lapenna A, Cohen-Daniel L, Tirosh B, Berger M
Oncotarget7(26)39396-39407(2016 Jun)

Expression and regulatory effects of murine Schlafen (Slfn) genes in malignant melanoma and renal cell carcinoma.
Mavrommatis E, Arslan AD, Sassano A, Hua Y, Kroczynska B, Platanias LC
The Journal of biological chemistry288(46)33006-15(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulation of cholesterol levels through the prevention of endoplasmic reticulum stress is an essential component of the mechanism by which Slfn2 regulates T-cell quiescence.
Title: Slfn2 mutation-induced loss of T-cell quiescence leads to elevated de novo sterol synthesis.

there is a functional connection between Slfn2 induced quiescence in T cells and endoplasmic reticulum homeostasis, which shows a novel mechanism by which immune cell quiescence is maintained
Title: Schlafen2 mutation unravels a role for chronic ER stress in the loss of T cell quiescence.

several mouse Slfn genes are up-regulated in response to IFN treatment of mouse melanoma and renal cell carcinoma cells, including Slfn1, Slfn2, Slfn4, Slfn5, and Slfn8.
Title: Expression and regulatory effects of murine Schlafen (Slfn) genes in malignant melanoma and renal cell carcinoma.

Slfn2 completely blocked maturation of T cells but there was no difference between Slfn2 expressing and none expressing NK cells.
Title: Effect of Schlafen 2 on natural killer and T cell development from common T/natural killer progenitors.

Slfn2 acts as a regulator of T cell quiescence
Title: Quieting T cells with Slfn2.

The following Slfn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slfn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08118	NM_011408.1 Latest version!	Mus musculus schlafen 2 (Slfn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slfn2 ( NM_011408.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035538.1 Mus musculus schlafen 2 (Slfn2), mRNA.

Price & Availability↑

CloneID	OMu08118
Clone ID Related Accession (Same CDS sequence)	NM_011408.1
Accession Version	NM_011408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Mus musculus(house mouse)
Product	schlafen family member 12-like
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF099973.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF099973.1, BC138750.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTACTA GACTTGAGGC AACTGAGCAA AGCAACCACA GAACTCAGAG GAATGACATT 
CAGCTGGAAA ATGCAAAGGC TGCTGGGAAA ATGGGCATCA GTGTTGATCT GGAAGCCAAA 
TATGCTAAGC TGGGTCTAAA TCTCGGAGCA ATCACTTTTG GAGAGAAGGA TAGGAAGAAA 
ATGAAGAATT CTCACCTCAG AAAACAGGAG AATGCAAACA TCTCTCTAGC TGTATGTGCT 
CTCCTGAATT CGGGAGGTGG AGCAATCAAG GTTAAAATTG AAAATGAAAA TTATAGTCTC 
ACTAGGGATG GCCTGGGACT AGATTTGGAA GCCTCTCTTT GTAAATGTCT GCCCTTTGTC 
CAGTGGCACC TGGACTTCAC GGAGAGCGAA GGCTACATTT ATATCTACGT GAAATCGTGG 
AGCCAAGAAA TCTTTGGGCT GCCTATTGGC ACCCTAAGAA CCAATTTGTA TGTAAGGAGC 
ATGTCATCTT CTGTACAAGT GAGCGCCGCT GCTGCCCTGG AATTTCTCCA GGACCTGGAG 
GAAACTGGAG GGAGACCCTG TGTCAGACCA GAGTTGCCTG CAAGCATAGC CTTCCCTGAA 
GTGGAAGGAG AATGGCACCT GGAGGATTTG GCTGCTGCAT TGTTTAACAG GACAGAATTT 
CAGTACGAGG AAACTTTCCC CTTTACCAGA TCCAGATATG TTGAAGTTAC ATTGCTTTCA 
GCGAAACGCC TGCGAAAACG CATCAAAGAG CTCCTCCCTC AAACTGTTTC TGCTTTTGCA 
AACACGGATG GGGGATTTTT GTTCATTGGT TTGGATGGCA AAACCCAGCA AATTATTGGT 
TTTGAAGCAG AGAAGAGCGA TCTCGTGCTT CTAGAGAGTG AAATAGAAAA GCACATCCGG 
CAGCTGCCTG TCACTCACTT CTGTGAGGAG AAGGAGAAGA TCAAGTACAC GTGCAAATTC 
ATCGAAGTGC ACAAATCCGG AGCTGTGTGT GCATATGTGT GTGCGCTCAG AGTGGAGAGA 
TTCTGCTGTG CAGTATTCGC TGCAGAGCCC GAGTCCTGGC ATGTGGAAGG CGGCTGTGTG 
AAGAGGTTTA CCACAGAGGA ATGGGTGAAA CTCCAGATGA ATGCCCCATC AGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035538.1
CDS	183..1319
Translation	MGTRLEATEQSNHRTQRNDIQLENAKAAGKMGISVDLEAKYAKLGLNLGAITFGEKDRKKMKNSHLRKQENANISLAVCALLNSGGGAIKVKIENENYSLTRDGLGLDLEASLCKCLPFVQWHLDFTESEGYIYIYVKSWSQEIFGLPIGTLRTNLYVRSMSSSVQVSAAAALEFLQDLEETGGRPCVRPELPASIAFPEVEGEWHLEDLAAALFNRTEFQYEETFPFTRSRYVEVTLLSAKRLRKRIKELLPQTVSAFANTDGGFLFIGLDGKTQQIIGFEAEKSDLVLLESEIEKHIRQLPVTHFCEEKEKIKYTCKFIEVHKSGAVCAYVCALRVERFCCAVFAAEPESWHVEGGCVKRFTTEEWVKLQMNAPSG

Target ORF information:

RefSeq Version	NM_011408.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus schlafen 2 (Slfn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011408.1

ORF Insert Sequence:

ATGGGTACTA GACTTGAGGC AACTGAGCAA AGCAACCACA GAACTCAGAG GAATGACATT 
CAGCTGGAAA ATGCAAAGGC TGCTGGGAAA ATGGGCATCA GTGTTGATCT GGAAGCCAAA 
TATGCTAAGC TGGGTCTAAA TCTCGGAGCA ATCACTTTTG GAGAGAAGGA TAGGAAGAAA 
ATGAAGAATT CTCACCTCAG AAAACAGGAG AATGCAAACA TCTCTCTAGC TGTATGTGCT 
CTCCTGAATT CGGGAGGTGG AGCAATCAAG GTTAAAATTG AAAATGAAAA TTATAGTCTC 
ACTAGGGATG GCCTGGGACT AGATTTGGAA GCCTCTCTTT GTAAATGTCT GCCCTTTGTC 
CAGTGGCACC TGGACTTCAC GGAGAGCGAA GGCTACATTT ATATCTACGT GAAATCGTGG 
AGCCAAGAAA TCTTTGGGCT GCCTATTGGC ACCCTAAGAA CCAATTTGTA TGTAAGGAGC 
ATGTCATCTT CTGTACAAGT GAGCGCCGCT GCTGCCCTGG AATTTCTCCA GGACCTGGAG 
GAAACTGGAG GGAGACCCTG TGTCAGACCA GAGTTGCCTG CAAGCATAGC CTTCCCTGAA 
GTGGAAGGAG AATGGCACCT GGAGGATTTG GCTGCTGCAT TGTTTAACAG GACAGAATTT 
CAGTACGAGG AAACTTTCCC CTTTACCAGA TCCAGATATG TTGAAGTTAC ATTGCTTTCA 
GCGAAACGCC TGCGAAAACG CATCAAAGAG CTCCTCCCTC AAACTGTTTC TGCTTTTGCA 
AACACGGATG GGGGATTTTT GTTCATTGGT TTGGATGGCA AAACCCAGCA AATTATTGGT 
TTTGAAGCAG AGAAGAGCGA TCTCGTGCTT CTAGAGAGTG AAATAGAAAA GCACATCCGG 
CAGCTGCCTG TCACTCACTT CTGTGAGGAG AAGGAGAAGA TCAAGTACAC GTGCAAATTC 
ATCGAAGTGC ACAAATCCGG AGCTGTGTGT GCATATGTGT GTGCGCTCAG AGTGGAGAGA 
TTCTGCTGTG CAGTATTCGC TGCAGAGCCC GAGTCCTGGC ATGTGGAAGG CGGCTGTGTG 
AAGAGGTTTA CCACAGAGGA ATGGGTGAAA CTCCAGATGA ATGCCCCATC AGGTTGA