Nek1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017312611.1	XP_017168100.1	serine/threonine-protein kinase Nek1 isoform X5
XM_030243338.1	XP_030099198.1	serine/threonine-protein kinase Nek1 isoform X1
NM_175089.4	NP_780298.2	serine/threonine-protein kinase Nek1 isoform 3
NM_001293639.1	NP_001280568.1	serine/threonine-protein kinase Nek1 isoform 4
XM_006509294.2	XP_006509357.1	serine/threonine-protein kinase Nek1 isoform X3
NM_001293638.1	NP_001280567.1	serine/threonine-protein kinase Nek1 isoform 2
XM_006509294.3	XP_006509357.1	serine/threonine-protein kinase Nek1 isoform X2
XM_030243339.1	XP_030099199.1	serine/threonine-protein kinase Nek1 isoform X3
XM_030243340.1	XP_030099200.1	serine/threonine-protein kinase Nek1 isoform X4
XM_017312609.1	XP_017168098.1	serine/threonine-protein kinase Nek1 isoform X2
NM_001293637.1	NP_001280566.1	serine/threonine-protein kinase Nek1 isoform 1
XM_017312610.1	XP_017168099.1	serine/threonine-protein kinase Nek1 isoform X4
XM_017312608.1	XP_017168097.1	serine/threonine-protein kinase Nek1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40752	XM_006509294.3 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu40753	XM_030243339.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu68310	XM_030243338.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu82381	XM_017312611.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu82381	XM_030243340.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu23426	NM_175089.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu23428	NM_001293638.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu23429	NM_001293637.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu23430	NM_001293639.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu23429	XM_017312608.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu40753	XM_017312610.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu68310	XM_017312609.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu40752	XM_006509294.2	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

Nek1 ( XM_006509294.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509357.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu40752
Clone ID Related Accession (Same CDS sequence)	XM_006509294.2 , XM_006509294.3
Accession Version	XM_006509294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGTTTATTT GGCAAGACTG 
AGGCAAATAA GACTACAAAA TTTTAATGAG CGCCAACAGA TTAAAGCCAA ACTTCGTGGT 
GAGAATAAAG AAGCTGATGG TACCAAAGGA CAAGAAGCAA CTGAAGAGAC TGACATGAGG 
CTCAAAAAGA TGGAGTCACT TAAGGCGCAA ACAAATGCAC GTGCTGCTGT ACTAAAAGAA 
CAGCTGGAGC GAAAAAGAAA GGAAGCTTAT GAAAGAGAAA AGAAAGTATG GGAAGAACAT 
TTGGTGGCGA GGGTAAAAAG CTCAGATGTT CCTCTGCCTT TGGAACTTCT TGAAACAGGT 
GGTTCTCCAT CAAAGCAGCA GGTGAAGCCT GTCATTTCTG TGACTTCAGC TTTGAAAGAA 
GTGGGCCTGG ATGGAAGTTT AACTGATACC CAGGAAGAAG AAATGGAAAA GAGTAACAGT 
GCTATTTCAA GTAAGCGAGA AATCCTGCGT AGGCTAAATG AAAATCTTAA AGCTCAAGAG 
GATGAAAAGG AAAAGCAGCA TCACTCAGGT TCTTGTGAGA CCGTTGGTCA CAAAGATGAG 
AGAGAGTATG AGACAGAAAA TGCCATTTCC TCTGATCGCA AGAAGTGGGA GATGGGAGGT 
CAGCTTGTGA TTCCTCTCGA TGCAGTGACA CTGGATACAT CCTTCTCTGC AACCGAAAAA 
CATACTGTGG GAGAGGTTAT TAAATTAGAT TCTAATGGCT CTCCAAGAAA AGTCTGGGGG 
AAAAACCCTA CAGATTCTGT GCTGAAGATA CTTGGAGAAG CTGAATTACA GCTACAGACA 
GAACTACTAG AAAACACATC TTTTAAAAGT GAGGTTTATG CTGAAGAGGA GAACTACAAA 
CCCTTACTTA CTGAAGAAGA GAATCTGCAG TGCATTTCAA AAGAAATAAA TCCATCAGCT 
ACTGTTGATT CTACTGAAAC GAAAAGTCCA AAGTTTACTG AGGTGTCTCC ACAAATGTCA 
GAAGGAAATG TGGAAGAACC TGATGATTTG GAAACAGAAG TTCTACAAGA GCCAAGTAGC 
ACACACACAG ATGGGAGTTT GCCACCTGTT CTTAATGATG TGTGGACTAG AGAGAAGGAA 
GCAGCTAAGG AAACTGAGTT GGAAGATAAG GTTGCTGTGC AGCAGAGTGA AGTTTGTGAA 
GATAGAATTC CAGGGAACGT GGACCAATCC TGTAAGGATC AGAGAGATCC TGCAGTAGAC 
GATTCTCCGC AGTCTGGCTG TGATGTAGAG AAGTCAGTAC AGCCAGAATC GATTTTCCAG 
AAAGTGGTTC ATTCTAAGGA CTTGAACTTA GTTCAGGCAG TTCATTGCTC ACCAGAAGAA 
CCAATTCCAA TTCGATCTCA CTCTGATTCT CCACCAAAAA CTAAGAGCAA GAATTCCTTA 
CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAATC CAAAGATGCT GAGGACCTGC 
TCACTTCCAG ATCTTTCCAA GCTGTTCAGA ACCCTAATGG ACGTTCCCAC TGTGGGGGAC 
GTTCATCAAG ACAGTCTTGA AATCGATGAG CTGGAAGATG AACCAATTAA AGAAGGGCCT 
TCTGATTCCG AAGACACTGT ATTTGAAGAA ACTGACACAG ATTTACAAGA GCTTCAGGCC 
TCAATGGAGC AGCTGCTTAG GGAGCAACCA GGTGACGAAT ACAGTGAGGA GGAAGAGTCT 
GTTTTAAAAA GCAGCGATGT GGAGCAGACA GCAAGAGGGA CAGATGCCCC AGACGAGGAG 
GACAACCCCA GCAGCGAAAG CGCCCTGAAC GAGGAATGGC ACTCAGATAA TAGTGACGCT 
GAGACCACTA GTGAATGTGA ATATGACAGT GTCTTTAACC ATTTAGAGGA ACTAAGACTT 
CACTTGGAGC AAGAAATGGG CTTTGAAAAG TTCTTTGAGG TTTATGAGAA AGTAAAGGCT 
ATTCATGAGG ATGAAGATGA AAATATTGAA ATTTGTTCAA CAATAGTTGA GAATATTTTG 
GGCAATGAGC ACCAGCATCT CTATGCCAAG ATTCTGCATT TAGTCATGGC AGATGGAGCC 
TATCAGGAAG ATAATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509357.1
CDS	638..4381
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_006509294.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509294.3

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGTTTATTT GGCAAGACTG 
AGGCAAATAA GACTACAAAA TTTTAATGAG CGCCAACAGA TTAAAGCCAA ACTTCGTGGT 
GAGAATAAAG AAGCTGATGG TACCAAAGGA CAAGAAGCAA CTGAAGAGAC TGACATGAGG 
CTCAAAAAGA TGGAGTCACT TAAGGCGCAA ACAAATGCAC GTGCTGCTGT ACTAAAAGAA 
CAGCTGGAGC GAAAAAGAAA GGAAGCTTAT GAAAGAGAAA AGAAAGTATG GGAAGAACAT 
TTGGTGGCGA GGGTAAAAAG CTCAGATGTT CCTCTGCCTT TGGAACTTCT TGAAACAGGT 
GGTTCTCCAT CAAAGCAGCA GGTGAAGCCT GTCATTTCTG TGACTTCAGC TTTGAAAGAA 
GTGGGCCTGG ATGGAAGTTT AACTGATACC CAGGAAGAAG AAATGGAAAA GAGTAACAGT 
GCTATTTCAA GTAAGCGAGA AATCCTGCGT AGGCTAAATG AAAATCTTAA AGCTCAAGAG 
GATGAAAAGG AAAAGCAGCA TCACTCAGGT TCTTGTGAGA CCGTTGGTCA CAAAGATGAG 
AGAGAGTATG AGACAGAAAA TGCCATTTCC TCTGATCGCA AGAAGTGGGA GATGGGAGGT 
CAGCTTGTGA TTCCTCTCGA TGCAGTGACA CTGGATACAT CCTTCTCTGC AACCGAAAAA 
CATACTGTGG GAGAGGTTAT TAAATTAGAT TCTAATGGCT CTCCAAGAAA AGTCTGGGGG 
AAAAACCCTA CAGATTCTGT GCTGAAGATA CTTGGAGAAG CTGAATTACA GCTACAGACA 
GAACTACTAG AAAACACATC TTTTAAAAGT GAGGTTTATG CTGAAGAGGA GAACTACAAA 
CCCTTACTTA CTGAAGAAGA GAATCTGCAG TGCATTTCAA AAGAAATAAA TCCATCAGCT 
ACTGTTGATT CTACTGAAAC GAAAAGTCCA AAGTTTACTG AGGTGTCTCC ACAAATGTCA 
GAAGGAAATG TGGAAGAACC TGATGATTTG GAAACAGAAG TTCTACAAGA GCCAAGTAGC 
ACACACACAG ATGGGAGTTT GCCACCTGTT CTTAATGATG TGTGGACTAG AGAGAAGGAA 
GCAGCTAAGG AAACTGAGTT GGAAGATAAG GTTGCTGTGC AGCAGAGTGA AGTTTGTGAA 
GATAGAATTC CAGGGAACGT GGACCAATCC TGTAAGGATC AGAGAGATCC TGCAGTAGAC 
GATTCTCCGC AGTCTGGCTG TGATGTAGAG AAGTCAGTAC AGCCAGAATC GATTTTCCAG 
AAAGTGGTTC ATTCTAAGGA CTTGAACTTA GTTCAGGCAG TTCATTGCTC ACCAGAAGAA 
CCAATTCCAA TTCGATCTCA CTCTGATTCT CCACCAAAAA CTAAGAGCAA GAATTCCTTA 
CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAATC CAAAGATGCT GAGGACCTGC 
TCACTTCCAG ATCTTTCCAA GCTGTTCAGA ACCCTAATGG ACGTTCCCAC TGTGGGGGAC 
GTTCATCAAG ACAGTCTTGA AATCGATGAG CTGGAAGATG AACCAATTAA AGAAGGGCCT 
TCTGATTCCG AAGACACTGT ATTTGAAGAA ACTGACACAG ATTTACAAGA GCTTCAGGCC 
TCAATGGAGC AGCTGCTTAG GGAGCAACCA GGTGACGAAT ACAGTGAGGA GGAAGAGTCT 
GTTTTAAAAA GCAGCGATGT GGAGCAGACA GCAAGAGGGA CAGATGCCCC AGACGAGGAG 
GACAACCCCA GCAGCGAAAG CGCCCTGAAC GAGGAATGGC ACTCAGATAA TAGTGACGCT 
GAGACCACTA GTGAATGTGA ATATGACAGT GTCTTTAACC ATTTAGAGGA ACTAAGACTT 
CACTTGGAGC AAGAAATGGG CTTTGAAAAG TTCTTTGAGG TTTATGAGAA AGTAAAGGCT 
ATTCATGAGG ATGAAGATGA AAATATTGAA ATTTGTTCAA CAATAGTTGA GAATATTTTG 
GGCAATGAGC ACCAGCATCT CTATGCCAAG ATTCTGCATT TAGTCATGGC AGATGGAGCC 
TATCAGGAAG ATAATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_030243339.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099199.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu40753
Clone ID Related Accession (Same CDS sequence)	XM_030243339.1 , XM_017312610.1
Accession Version	XM_030243339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GCTTCCTTTT TTGGCATTGG AGGGGCTGTC TCTCCATCAC CGTGTTCTCC TCGAGGCCAG 
TATGAACATT ACCATGCCAT TTTTGACCAA ATGCAGCGGC TAAGAGCAGA AGATAATGAA 
GCAAGATGGA AGGGGGGAAT CTATGGTCGA TGGCTCCCAG AAAGAGGAGT TCCACCTGGA 
GTTCGTCCAG GATTTCCCAG TGAGGCTTCA GGTCACTACC ATTCCCCTGA TGCTGGTGCT 
ATTAGGAAAA CTCTGAAAAG ACTGAAGGCC GTGTCTAAGC AAGCCAGTAC AAACAGGCAA 
AAAGGACACT TAGCTGTAGA GAGAGCCAAC CAAGTGGAAG AATTCCTACA GCGTAAACGA 
GAAGCTATGC AGAATAAAGC CCGAGCCGAA GGACACGTGG GACTCCTGCA AAACCTGGCA 
TCTCTGTATG GAGGCAGGCC CAGCTCTTCA AGAGGAGGGA AGCCAAGGAA CAATGAGGAA 
GAGGTTTATT TGGCAAGACT GAGGCAAATA AGACTACAAA ATTTTAATGA GCGCCAACAG 
ATTAAAGCCA AACTTCGTGG TGAGAATAAA GAAGCTGATG GTACCAAAGG ACAAGAAGCA 
ACTGAAGAGA CTGACATGAG GCTCAAAAAG ATGGAGTCAC TTAAGGCGCA AACAAATGCA 
CGTGCTGCTG TACTAAAAGA ACAGCTGGAG CGAAAAAGAA AGGAAGCTTA TGAAAGAGAA 
AAGAAAGTAT GGGAAGAACA TTTGGTGGCG AGGGTAAAAA GCTCAGATGT TCCTCTGCCT 
TTGGAACTTC TTGAAACAGG TGGTTCTCCA TCAAAGCAGC AGGTGAAGCC TGTCATTTCT 
GTGACTTCAG CTTTGAAAGA AGTGGGCCTG GATGGAAGTT TAACTGATAC CCAGGAAGAA 
GAAATGGAAA AGAGTAACAG TGCTATTTCA AGTAAGCGAG AAATCCTGCG TAGGCTAAAT 
GAAAATCTTA AAGCTCAAGA GGATGAAAAG GAAAAGCAGC ATCACTCAGG TTCTTGTGAG 
ACCGTTGGTC ACAAAGATGA GAGAGAGTAT GAGACAGAAA ATGCCATTTC CTCTGATCGC 
AAGAAGTGGG AGATGGGAGG TCAGCTTGTG ATTCCTCTCG ATGCAGTGAC ACTGGATACA 
TCCTTCTCTG CAACCGAAAA ACATACTGTG GGAGAGGTTA TTAAATTAGA TTCTAATGGC 
TCTCCAAGAA AAGTCTGGGG GAAAAACCCT ACAGATTCTG TGCTGAAGAT ACTTGGAGAA 
GCTGAATTAC AGCTACAGAC AGAACTACTA GAAAACACAT CTTTTAAAAG TGAGGTTTAT 
GCTGAAGAGG AGAACTACAA ACCCTTACTT ACTGAAGAAG AGAATCTGCA GTGCATTTCA 
AAAGAAATAA ATCCATCAGC TACTGTTGAT TCTACTGAAA CGAAAAGTCC AAAGTTTACT 
GAGGTGTCTC CACAAATGTC AGAAGGAAAT GTGGAAGAAC CTGATGATTT GGAAACAGAA 
GTTCTACAAG AGCCAAGTAG CACACACACA GATGGGAGTT TGCCACCTGT TCTTAATGAT 
GTGTGGACTA GAGAGAAGGA AGCAGCTAAG GAAACTGAGT TGGAAGATAA GGTTGCTGTG 
CAGCAGAGTG AAGTTTGTGA AGATAGAATT CCAGGGAACG TGGACCAATC CTGTAAGGAT 
CAGAGAGATC CTGCAGTAGA CGATTCTCCG CAGTCTGGCT GTGATGTAGA GAAGTCAGTA 
CAGCCAGAAT CGATTTTCCA GAAAGTGGTT CATTCTAAGG ACTTGAACTT AGTTCAGGCA 
GTTCATTGCT CACCAGAAGA ACCAATTCCA ATTCGATCTC ACTCTGATTC TCCACCAAAA 
ACTAAGAGCA AGAATTCCTT ACTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAT 
CCAAAGATGC TGAGGACCTG CTCACTTCCA GATCTTTCCA AGCTGTTCAG AACCCTAATG 
GACGTTCCCA CTGTGGGGGA CGTTCATCAA GACAGTCTTG AAATCGATGA GCTGGAAGAT 
GAACCAATTA AAGAAGGGCC TTCTGATTCC GAAGACACTG TATTTGAAGA AACTGACACA 
GATTTACAAG AGCTTCAGGC CTCAATGGAG CAGCTGCTTA GGGAGCAACC AGGTGACGAA 
TACAGTGAGG AGGAAGAGTC TGTTTTAAAA AGCAGCGATG TGGAGCAGAC AGCAAGAGGG 
ACAGATGCCC CAGACGAGGA GGACAACCCC AGCAGCGAAA GCGCCCTGAA CGAGGAATGG 
CACTCAGATA ATAGTGACGC TGAGACCACT AGTGAATGTG AATATGACAG TGTCTTTAAC 
CATTTAGAGG AACTAAGACT TCACTTGGAG CAAGAAATGG GCTTTGAAAA GTTCTTTGAG 
GTTTATGAGA AAGTAAAGGC TATTCATGAG GATGAAGATG AAAATATTGA AATTTGTTCA 
ACAATAGTTG AGAATATTTT GGGCAATGAG CACCAGCATC TCTATGCCAA GATTCTGCAT 
TTAGTCATGG CAGATGGAGC CTATCAGGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099199.1
CDS	639..4283
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_030243339.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243339.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GCTTCCTTTT TTGGCATTGG AGGGGCTGTC TCTCCATCAC CGTGTTCTCC TCGAGGCCAG 
TATGAACATT ACCATGCCAT TTTTGACCAA ATGCAGCGGC TAAGAGCAGA AGATAATGAA 
GCAAGATGGA AGGGGGGAAT CTATGGTCGA TGGCTCCCAG AAAGAGGAGT TCCACCTGGA 
GTTCGTCCAG GATTTCCCAG TGAGGCTTCA GGTCACTACC ATTCCCCTGA TGCTGGTGCT 
ATTAGGAAAA CTCTGAAAAG ACTGAAGGCC GTGTCTAAGC AAGCCAGTAC AAACAGGCAA 
AAAGGACACT TAGCTGTAGA GAGAGCCAAC CAAGTGGAAG AATTCCTACA GCGTAAACGA 
GAAGCTATGC AGAATAAAGC CCGAGCCGAA GGACACGTGG GACTCCTGCA AAACCTGGCA 
TCTCTGTATG GAGGCAGGCC CAGCTCTTCA AGAGGAGGGA AGCCAAGGAA CAATGAGGAA 
GAGGTTTATT TGGCAAGACT GAGGCAAATA AGACTACAAA ATTTTAATGA GCGCCAACAG 
ATTAAAGCCA AACTTCGTGG TGAGAATAAA GAAGCTGATG GTACCAAAGG ACAAGAAGCA 
ACTGAAGAGA CTGACATGAG GCTCAAAAAG ATGGAGTCAC TTAAGGCGCA AACAAATGCA 
CGTGCTGCTG TACTAAAAGA ACAGCTGGAG CGAAAAAGAA AGGAAGCTTA TGAAAGAGAA 
AAGAAAGTAT GGGAAGAACA TTTGGTGGCG AGGGTAAAAA GCTCAGATGT TCCTCTGCCT 
TTGGAACTTC TTGAAACAGG TGGTTCTCCA TCAAAGCAGC AGGTGAAGCC TGTCATTTCT 
GTGACTTCAG CTTTGAAAGA AGTGGGCCTG GATGGAAGTT TAACTGATAC CCAGGAAGAA 
GAAATGGAAA AGAGTAACAG TGCTATTTCA AGTAAGCGAG AAATCCTGCG TAGGCTAAAT 
GAAAATCTTA AAGCTCAAGA GGATGAAAAG GAAAAGCAGC ATCACTCAGG TTCTTGTGAG 
ACCGTTGGTC ACAAAGATGA GAGAGAGTAT GAGACAGAAA ATGCCATTTC CTCTGATCGC 
AAGAAGTGGG AGATGGGAGG TCAGCTTGTG ATTCCTCTCG ATGCAGTGAC ACTGGATACA 
TCCTTCTCTG CAACCGAAAA ACATACTGTG GGAGAGGTTA TTAAATTAGA TTCTAATGGC 
TCTCCAAGAA AAGTCTGGGG GAAAAACCCT ACAGATTCTG TGCTGAAGAT ACTTGGAGAA 
GCTGAATTAC AGCTACAGAC AGAACTACTA GAAAACACAT CTTTTAAAAG TGAGGTTTAT 
GCTGAAGAGG AGAACTACAA ACCCTTACTT ACTGAAGAAG AGAATCTGCA GTGCATTTCA 
AAAGAAATAA ATCCATCAGC TACTGTTGAT TCTACTGAAA CGAAAAGTCC AAAGTTTACT 
GAGGTGTCTC CACAAATGTC AGAAGGAAAT GTGGAAGAAC CTGATGATTT GGAAACAGAA 
GTTCTACAAG AGCCAAGTAG CACACACACA GATGGGAGTT TGCCACCTGT TCTTAATGAT 
GTGTGGACTA GAGAGAAGGA AGCAGCTAAG GAAACTGAGT TGGAAGATAA GGTTGCTGTG 
CAGCAGAGTG AAGTTTGTGA AGATAGAATT CCAGGGAACG TGGACCAATC CTGTAAGGAT 
CAGAGAGATC CTGCAGTAGA CGATTCTCCG CAGTCTGGCT GTGATGTAGA GAAGTCAGTA 
CAGCCAGAAT CGATTTTCCA GAAAGTGGTT CATTCTAAGG ACTTGAACTT AGTTCAGGCA 
GTTCATTGCT CACCAGAAGA ACCAATTCCA ATTCGATCTC ACTCTGATTC TCCACCAAAA 
ACTAAGAGCA AGAATTCCTT ACTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAT 
CCAAAGATGC TGAGGACCTG CTCACTTCCA GATCTTTCCA AGCTGTTCAG AACCCTAATG 
GACGTTCCCA CTGTGGGGGA CGTTCATCAA GACAGTCTTG AAATCGATGA GCTGGAAGAT 
GAACCAATTA AAGAAGGGCC TTCTGATTCC GAAGACACTG TATTTGAAGA AACTGACACA 
GATTTACAAG AGCTTCAGGC CTCAATGGAG CAGCTGCTTA GGGAGCAACC AGGTGACGAA 
TACAGTGAGG AGGAAGAGTC TGTTTTAAAA AGCAGCGATG TGGAGCAGAC AGCAAGAGGG 
ACAGATGCCC CAGACGAGGA GGACAACCCC AGCAGCGAAA GCGCCCTGAA CGAGGAATGG 
CACTCAGATA ATAGTGACGC TGAGACCACT AGTGAATGTG AATATGACAG TGTCTTTAAC 
CATTTAGAGG AACTAAGACT TCACTTGGAG CAAGAAATGG GCTTTGAAAA GTTCTTTGAG 
GTTTATGAGA AAGTAAAGGC TATTCATGAG GATGAAGATG AAAATATTGA AATTTGTTCA 
ACAATAGTTG AGAATATTTT GGGCAATGAG CACCAGCATC TCTATGCCAA GATTCTGCAT 
TTAGTCATGG CAGATGGAGC CTATCAGGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_030243338.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099198.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X1, mRNA.

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CloneID	OMu68310
Clone ID Related Accession (Same CDS sequence)	XM_030243338.1 , XM_017312609.1
Accession Version	XM_030243338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGGC TTCCTTTTTT 
GGCATTGGAG GGGCTGTCTC TCCATCACCG TGTTCTCCTC GAGGCCAGTA TGAACATTAC 
CATGCCATTT TTGACCAAAT GCAGCGGCTA AGAGCAGAAG ATAATGAAGC AAGATGGAAG 
GGGGGAATCT ATGGTCGATG GCTCCCAGAA AGAGGAGTTC CACCTGGAGT TCGTCCAGGA 
TTTCCCAGTG AGGCTTCAGG TCACTACCAT TCCCCTGATG CTGGTGCTAT TAGGAAAACT 
CTGAAAAGAC TGAAGGCCGT GTCTAAGCAA GCCAGTACAA ACAGGCAAAA AGGACACTTA 
GCTGTAGAGA GAGCCAACCA AGTGGAAGAA TTCCTACAGC GTAAACGAGA AGCTATGCAG 
AATAAAGCCC GAGCCGAAGG ACACGTGGGA CTCCTGCAAA ACCTGGCATC TCTGTATGGA 
GGCAGGCCCA GCTCTTCAAG AGGAGGGAAG CCAAGGAACA ATGAGGAAGA GGTTTATTTG 
GCAAGACTGA GGCAAATAAG ACTACAAAAT TTTAATGAGC GCCAACAGAT TAAAGCCAAA 
CTTCGTGGTG AGAATAAAGA AGCTGATGGT ACCAAAGGAC AAGAAGCAAC TGAAGAGACT 
GACATGAGGC TCAAAAAGAT GGAGTCACTT AAGGCGCAAA CAAATGCACG TGCTGCTGTA 
CTAAAAGAAC AGCTGGAGCG AAAAAGAAAG GAAGCTTATG AAAGAGAAAA GAAAGTATGG 
GAAGAACATT TGGTGGCGAG GGTAAAAAGC TCAGATGTTC CTCTGCCTTT GGAACTTCTT 
GAAACAGGTG GTTCTCCATC AAAGCAGCAG GTGAAGCCTG TCATTTCTGT GACTTCAGCT 
TTGAAAGAAG TGGGCCTGGA TGGAAGTTTA ACTGATACCC AGGAAGAAGA AATGGAAAAG 
AGTAACAGTG CTATTTCAAG TAAGCGAGAA ATCCTGCGTA GGCTAAATGA AAATCTTAAA 
GCTCAAGAGG ATGAAAAGGA AAAGCAGCAT CACTCAGGTT CTTGTGAGAC CGTTGGTCAC 
AAAGATGAGA GAGAGTATGA GACAGAAAAT GCCATTTCCT CTGATCGCAA GAAGTGGGAG 
ATGGGAGGTC AGCTTGTGAT TCCTCTCGAT GCAGTGACAC TGGATACATC CTTCTCTGCA 
ACCGAAAAAC ATACTGTGGG AGAGGTTATT AAATTAGATT CTAATGGCTC TCCAAGAAAA 
GTCTGGGGGA AAAACCCTAC AGATTCTGTG CTGAAGATAC TTGGAGAAGC TGAATTACAG 
CTACAGACAG AACTACTAGA AAACACATCT TTTAAAAGTG AGGTTTATGC TGAAGAGGAG 
AACTACAAAC CCTTACTTAC TGAAGAAGAG AATCTGCAGT GCATTTCAAA AGAAATAAAT 
CCATCAGCTA CTGTTGATTC TACTGAAACG AAAAGTCCAA AGTTTACTGA GGTGTCTCCA 
CAAATGTCAG AAGGAAATGT GGAAGAACCT GATGATTTGG AAACAGAAGT TCTACAAGAG 
CCAAGTAGCA CACACACAGA TGGGAGTTTG CCACCTGTTC TTAATGATGT GTGGACTAGA 
GAGAAGGAAG CAGCTAAGGA AACTGAGTTG GAAGATAAGG TTGCTGTGCA GCAGAGTGAA 
GTTTGTGAAG ATAGAATTCC AGGGAACGTG GACCAATCCT GTAAGGATCA GAGAGATCCT 
GCAGTAGACG ATTCTCCGCA GTCTGGCTGT GATGTAGAGA AGTCAGTACA GCCAGAATCG 
ATTTTCCAGA AAGTGGTTCA TTCTAAGGAC TTGAACTTAG TTCAGGCAGT TCATTGCTCA 
CCAGAAGAAC CAATTCCAAT TCGATCTCAC TCTGATTCTC CACCAAAAAC TAAGAGCAAG 
AATTCCTTAC TGATTGGACT TTCAACTGGT CTGTTTGATG CAAACAATCC AAAGATGCTG 
AGGACCTGCT CACTTCCAGA TCTTTCCAAG CTGTTCAGAA CCCTAATGGA CGTTCCCACT 
GTGGGGGACG TTCATCAAGA CAGTCTTGAA ATCGATGAGC TGGAAGATGA ACCAATTAAA 
GAAGGGCCTT CTGATTCCGA AGACACTGTA TTTGAAGAAA CTGACACAGA TTTACAAGAG 
CTTCAGGCCT CAATGGAGCA GCTGCTTAGG GAGCAACCAG GTGACGAATA CAGTGAGGAG 
GAAGAGTCTG TTTTAAAAAG CAGCGATGTG GAGCAGACAG CAAGAGGGAC AGATGCCCCA 
GACGAGGAGG ACAACCCCAG CAGCGAAAGC GCCCTGAACG AGGAATGGCA CTCAGATAAT 
AGTGACGCTG AGACCACTAG TGAATGTGAA TATGACAGTG TCTTTAACCA TTTAGAGGAA 
CTAAGACTTC ACTTGGAGCA AGAAATGGGC TTTGAAAAGT TCTTTGAGGT TTATGAGAAA 
GTAAAGGCTA TTCATGAGGA TGAAGATGAA AATATTGAAA TTTGTTCAAC AATAGTTGAG 
AATATTTTGG GCAATGAGCA CCAGCATCTC TATGCCAAGA TTCTGCATTT AGTCATGGCA 
GATGGAGCCT ATCAGGAAGA TAATGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099198.1
CDS	639..4391
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_030243338.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243338.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGGC TTCCTTTTTT 
GGCATTGGAG GGGCTGTCTC TCCATCACCG TGTTCTCCTC GAGGCCAGTA TGAACATTAC 
CATGCCATTT TTGACCAAAT GCAGCGGCTA AGAGCAGAAG ATAATGAAGC AAGATGGAAG 
GGGGGAATCT ATGGTCGATG GCTCCCAGAA AGAGGAGTTC CACCTGGAGT TCGTCCAGGA 
TTTCCCAGTG AGGCTTCAGG TCACTACCAT TCCCCTGATG CTGGTGCTAT TAGGAAAACT 
CTGAAAAGAC TGAAGGCCGT GTCTAAGCAA GCCAGTACAA ACAGGCAAAA AGGACACTTA 
GCTGTAGAGA GAGCCAACCA AGTGGAAGAA TTCCTACAGC GTAAACGAGA AGCTATGCAG 
AATAAAGCCC GAGCCGAAGG ACACGTGGGA CTCCTGCAAA ACCTGGCATC TCTGTATGGA 
GGCAGGCCCA GCTCTTCAAG AGGAGGGAAG CCAAGGAACA ATGAGGAAGA GGTTTATTTG 
GCAAGACTGA GGCAAATAAG ACTACAAAAT TTTAATGAGC GCCAACAGAT TAAAGCCAAA 
CTTCGTGGTG AGAATAAAGA AGCTGATGGT ACCAAAGGAC AAGAAGCAAC TGAAGAGACT 
GACATGAGGC TCAAAAAGAT GGAGTCACTT AAGGCGCAAA CAAATGCACG TGCTGCTGTA 
CTAAAAGAAC AGCTGGAGCG AAAAAGAAAG GAAGCTTATG AAAGAGAAAA GAAAGTATGG 
GAAGAACATT TGGTGGCGAG GGTAAAAAGC TCAGATGTTC CTCTGCCTTT GGAACTTCTT 
GAAACAGGTG GTTCTCCATC AAAGCAGCAG GTGAAGCCTG TCATTTCTGT GACTTCAGCT 
TTGAAAGAAG TGGGCCTGGA TGGAAGTTTA ACTGATACCC AGGAAGAAGA AATGGAAAAG 
AGTAACAGTG CTATTTCAAG TAAGCGAGAA ATCCTGCGTA GGCTAAATGA AAATCTTAAA 
GCTCAAGAGG ATGAAAAGGA AAAGCAGCAT CACTCAGGTT CTTGTGAGAC CGTTGGTCAC 
AAAGATGAGA GAGAGTATGA GACAGAAAAT GCCATTTCCT CTGATCGCAA GAAGTGGGAG 
ATGGGAGGTC AGCTTGTGAT TCCTCTCGAT GCAGTGACAC TGGATACATC CTTCTCTGCA 
ACCGAAAAAC ATACTGTGGG AGAGGTTATT AAATTAGATT CTAATGGCTC TCCAAGAAAA 
GTCTGGGGGA AAAACCCTAC AGATTCTGTG CTGAAGATAC TTGGAGAAGC TGAATTACAG 
CTACAGACAG AACTACTAGA AAACACATCT TTTAAAAGTG AGGTTTATGC TGAAGAGGAG 
AACTACAAAC CCTTACTTAC TGAAGAAGAG AATCTGCAGT GCATTTCAAA AGAAATAAAT 
CCATCAGCTA CTGTTGATTC TACTGAAACG AAAAGTCCAA AGTTTACTGA GGTGTCTCCA 
CAAATGTCAG AAGGAAATGT GGAAGAACCT GATGATTTGG AAACAGAAGT TCTACAAGAG 
CCAAGTAGCA CACACACAGA TGGGAGTTTG CCACCTGTTC TTAATGATGT GTGGACTAGA 
GAGAAGGAAG CAGCTAAGGA AACTGAGTTG GAAGATAAGG TTGCTGTGCA GCAGAGTGAA 
GTTTGTGAAG ATAGAATTCC AGGGAACGTG GACCAATCCT GTAAGGATCA GAGAGATCCT 
GCAGTAGACG ATTCTCCGCA GTCTGGCTGT GATGTAGAGA AGTCAGTACA GCCAGAATCG 
ATTTTCCAGA AAGTGGTTCA TTCTAAGGAC TTGAACTTAG TTCAGGCAGT TCATTGCTCA 
CCAGAAGAAC CAATTCCAAT TCGATCTCAC TCTGATTCTC CACCAAAAAC TAAGAGCAAG 
AATTCCTTAC TGATTGGACT TTCAACTGGT CTGTTTGATG CAAACAATCC AAAGATGCTG 
AGGACCTGCT CACTTCCAGA TCTTTCCAAG CTGTTCAGAA CCCTAATGGA CGTTCCCACT 
GTGGGGGACG TTCATCAAGA CAGTCTTGAA ATCGATGAGC TGGAAGATGA ACCAATTAAA 
GAAGGGCCTT CTGATTCCGA AGACACTGTA TTTGAAGAAA CTGACACAGA TTTACAAGAG 
CTTCAGGCCT CAATGGAGCA GCTGCTTAGG GAGCAACCAG GTGACGAATA CAGTGAGGAG 
GAAGAGTCTG TTTTAAAAAG CAGCGATGTG GAGCAGACAG CAAGAGGGAC AGATGCCCCA 
GACGAGGAGG ACAACCCCAG CAGCGAAAGC GCCCTGAACG AGGAATGGCA CTCAGATAAT 
AGTGACGCTG AGACCACTAG TGAATGTGAA TATGACAGTG TCTTTAACCA TTTAGAGGAA 
CTAAGACTTC ACTTGGAGCA AGAAATGGGC TTTGAAAAGT TCTTTGAGGT TTATGAGAAA 
GTAAAGGCTA TTCATGAGGA TGAAGATGAA AATATTGAAA TTTGTTCAAC AATAGTTGAG 
AATATTTTGG GCAATGAGCA CCAGCATCTC TATGCCAAGA TTCTGCATTT AGTCATGGCA 
GATGGAGCCT ATCAGGAAGA TAATGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_017312611.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168100.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu82381
Clone ID Related Accession (Same CDS sequence)	XM_017312611.1 , XM_030243340.1
Accession Version	XM_017312611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTACT GTGAAGGAGG TGATTTGTTT AAACGAATAA ATGCTCAGAA AGGCGCTCTG 
TTTCAAGAAG ACCAGATTTT GGACTGGTTT GTGCAGATAT GTTTGGCTCT GAAGCATGTA 
CATGATAGAA AAATTCTTCA CCGAGACATA AAGTCACAGA ACATATTTCT AACCAAAGAT 
GGGACAGTGC AGCTTGGAGA TTTTGGAATT GCTCGAGTTC TTAATAGTAC TGTAGAGCTG 
GCTCGAACTT GCATAGGCAC TCCATACTAC TTGTCACCTG AAATCTGTGA AAACAAGCCT 
TATAACAATA AAAGTGACAT TTGGGCTTTG GGCTGTGTCC TTTATGAGTT GTGTACACTT 
AAACATGCAT TTGAAGCTGG AAACATGAAA AACCTGGTAC TGAAGATAAT CTCCGGATCC 
TTTCCTCCAG TGTCTCCACA TTACTCCTAT GATCTCCGCA GCTTGCTGTC TCAGTTATTT 
AAAAGAAATC CTAGGGATAG ACCATCAGTC AACTCCATAT TGGAGAAAGG TTTTATAGCT 
AAACGAATCG AAAAGTTTCT CTCCCCTCAG CTTATTGCAG AAGAATTTTG TCTAAAAACA 
CTTTCAAAGT TTGGACCACA GCCTCTCCCA GGTAAAAGAC CAGCATCAGG ACAAGGTGTC 
AGTTCTTTTG TCCCTGCTCA GAAAATCACA AAGCCTGCTG CTAAATACGG AGTGCCTTTA 
ACATATAAGA AGTATGGAGA TAAAAAGTTA CTTGAGAAAA AACCACCCCC AAAACATAAA 
CAGGCCCATC AAATTCCCGT GAAGAAAATG AATTCTGGAG AAGAAAGGAA GAAAATGTCT 
GAGGAAGCAG CAAAAAAAAG AAGGTTGGAA TTTATTGAGA AAGAAAAGAA GCAAAAGGAT 
CAGATTAGGT TCCTGAAGGC TGAGCAGATG AAGCGGCAAG AGAAGCAGCG GTTGGAGAGG 
ATAAATAGGG CCAGGGAACA AGGATGGAGG AATGTTTTAA GGGCTGGTGG AAGCGGTGAA 
GTAAAGGCTT CCTTTTTTGG CATTGGAGGG GCTGTCTCTC CATCACCGTG TTCTCCTCGA 
GGCCAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCGGCTAAG AGCAGAAGAT 
AATGAAGCAA GATGGAAGGG GGGAATCTAT GGTCGATGGC TCCCAGAAAG AGGAGTTCCA 
CCTGGAGTTC GTCCAGGATT TCCCAGTGAG GCTTCAGGTC ACTACCATTC CCCTGATGCT 
GGTGCTATTA GGAAAACTCT GAAAAGACTG AAGGCCGTGT CTAAGCAAGC CAGTACAAAC 
AGGCAAAAAG GACACTTAGC TGTAGAGAGA GCCAACCAAG TGGAAGAATT CCTACAGCGT 
AAACGAGAAG CTATGCAGAA TAAAGCCCGA GCCGAAGGAC ACGTGGGACT CCTGCAAAAC 
CTGGCATCTC TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAT 
GAGGAAGAGG TTTATTTGGC AAGACTGAGG CAAATAAGAC TACAAAATTT TAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAG AATAAAGAAG CTGATGGTAC CAAAGGACAA 
GAAGCAACTG AAGAGACTGA CATGAGGCTC AAAAAGATGG AGTCACTTAA GGCGCAAACA 
AATGCACGTG CTGCTGTACT AAAAGAACAG CTGGAGCGAA AAAGAAAGGA AGCTTATGAA 
AGAGAAAAGA AAGTATGGGA AGAACATTTG GTGGCGAGGG TAAAAAGCTC AGATGTTCCT 
CTGCCTTTGG AACTTCTTGA AACAGGTGGT TCTCCATCAA AGCAGCAGGT GAAGCCTGTC 
ATTTCTGTGA CTTCAGCTTT GAAAGAAGTG GGCCTGGATG GAAGTTTAAC TGATACCCAG 
GAAGAAGAAA TGGAAAAGAG TAACAGTGCT ATTTCAAGTA AGCGAGAAAT CCTGCGTAGG 
CTAAATGAAA ATCTTAAAGC TCAAGAGGAT GAAAAGGAAA AGCAGCATCA CTCAGGTTCT 
TGTGAGACCG TTGGTCACAA AGATGAGAGA GAGTATGAGA CAGAAAATGC CATTTCCTCT 
GATCGCAAGA AGTGGGAGAT GGGAGGTCAG CTTGTGATTC CTCTCGATGC AGTGACACTG 
GATACATCCT TCTCTGCAAC CGAAAAACAT ACTGTGGGAG AGGTTATTAA ATTAGATTCT 
AATGGCTCTC CAAGAAAAGT CTGGGGGAAA AACCCTACAG ATTCTGTGCT GAAGATACTT 
GGAGAAGCTG AATTACAGCT ACAGACAGAA CTACTAGAAA ACACATCTTT TAAAAGTGAG 
GTTTATGCTG AAGAGGAGAA CTACAAACCC TTACTTACTG AAGAAGAGAA TCTGCAGTGC 
ATTTCAAAAG AAATAAATCC ATCAGCTACT GTTGATTCTA CTGAAACGAA AAGTCCAAAG 
TTTACTGAGG TGTCTCCACA AATGTCAGAA GGAAATGTGG AAGAACCTGA TGATTTGGAA 
ACAGAAGTTC TACAAGAGCC AAGTAGCACA CACACAGATG GGAGTTTGCC ACCTGTTCTT 
AATGATGTGT GGACTAGAGA GAAGGAAGCA GCTAAGGAAA CTGAGTTGGA AGATAAGGTT 
GCTGTGCAGC AGAGTGAAGT TTGTGAAGAT AGAATTCCAG GGAACGTGGA CCAATCCTGT 
AAGGATCAGA GAGATCCTGC AGTAGACGAT TCTCCGCAGT CTGGCTGTGA TGTAGAGAAG 
TCAGTACAGC CAGAATCGAT TTTCCAGAAA GTGGTTCATT CTAAGGACTT GAACTTAGTT 
CAGGCAGTTC ATTGCTCACC AGAAGAACCA ATTCCAATTC GATCTCACTC TGATTCTCCA 
CCAAAAACTA AGAGCAAGAA TTCCTTACTG ATTGGACTTT CAACTGGTCT GTTTGATGCA 
AACAATCCAA AGATGCTGAG GACCTGCTCA CTTCCAGATC TTTCCAAGCT GTTCAGAACC 
CTAATGGACG TTCCCACTGT GGGGGACGTT CATCAAGACA GTCTTGAAAT CGATGAGCTG 
GAAGATGAAC CAATTAAAGA AGGGCCTTCT GATTCCGAAG ACACTGTATT TGAAGAAACT 
GACACAGATT TACAAGAGCT TCAGGCCTCA ATGGAGCAGC TGCTTAGGGA GCAACCAGGT 
GACGAATACA GTGAGGAGGA AGAGTCTGTT TTAAAAAGCA GCGATGTGGA GCAGACAGCA 
AGAGGGACAG ATGCCCCAGA CGAGGAGGAC AACCCCAGCA GCGAAAGCGC CCTGAACGAG 
GAATGGCACT CAGATAATAG TGACGCTGAG ACCACTAGTG AATGTGAATA TGACAGTGTC 
TTTAACCATT TAGAGGAACT AAGACTTCAC TTGGAGCAAG AAATGGGCTT TGAAAAGTTC 
TTTGAGGTTT ATGAGAAAGT AAAGGCTATT CATGAGGATG AAGATGAAAA TATTGAAATT 
TGTTCAACAA TAGTTGAGAA TATTTTGGGC AATGAGCACC AGCATCTCTA TGCCAAGATT 
CTGCATTTAG TCATGGCAGA TGGAGCCTAT CAGGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168100.1
CDS	155..3745
Translation	MDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017312611.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312611.1

ORF Insert Sequence:

ATGGATTACT GTGAAGGAGG TGATTTGTTT AAACGAATAA ATGCTCAGAA AGGCGCTCTG 
TTTCAAGAAG ACCAGATTTT GGACTGGTTT GTGCAGATAT GTTTGGCTCT GAAGCATGTA 
CATGATAGAA AAATTCTTCA CCGAGACATA AAGTCACAGA ACATATTTCT AACCAAAGAT 
GGGACAGTGC AGCTTGGAGA TTTTGGAATT GCTCGAGTTC TTAATAGTAC TGTAGAGCTG 
GCTCGAACTT GCATAGGCAC TCCATACTAC TTGTCACCTG AAATCTGTGA AAACAAGCCT 
TATAACAATA AAAGTGACAT TTGGGCTTTG GGCTGTGTCC TTTATGAGTT GTGTACACTT 
AAACATGCAT TTGAAGCTGG AAACATGAAA AACCTGGTAC TGAAGATAAT CTCCGGATCC 
TTTCCTCCAG TGTCTCCACA TTACTCCTAT GATCTCCGCA GCTTGCTGTC TCAGTTATTT 
AAAAGAAATC CTAGGGATAG ACCATCAGTC AACTCCATAT TGGAGAAAGG TTTTATAGCT 
AAACGAATCG AAAAGTTTCT CTCCCCTCAG CTTATTGCAG AAGAATTTTG TCTAAAAACA 
CTTTCAAAGT TTGGACCACA GCCTCTCCCA GGTAAAAGAC CAGCATCAGG ACAAGGTGTC 
AGTTCTTTTG TCCCTGCTCA GAAAATCACA AAGCCTGCTG CTAAATACGG AGTGCCTTTA 
ACATATAAGA AGTATGGAGA TAAAAAGTTA CTTGAGAAAA AACCACCCCC AAAACATAAA 
CAGGCCCATC AAATTCCCGT GAAGAAAATG AATTCTGGAG AAGAAAGGAA GAAAATGTCT 
GAGGAAGCAG CAAAAAAAAG AAGGTTGGAA TTTATTGAGA AAGAAAAGAA GCAAAAGGAT 
CAGATTAGGT TCCTGAAGGC TGAGCAGATG AAGCGGCAAG AGAAGCAGCG GTTGGAGAGG 
ATAAATAGGG CCAGGGAACA AGGATGGAGG AATGTTTTAA GGGCTGGTGG AAGCGGTGAA 
GTAAAGGCTT CCTTTTTTGG CATTGGAGGG GCTGTCTCTC CATCACCGTG TTCTCCTCGA 
GGCCAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCGGCTAAG AGCAGAAGAT 
AATGAAGCAA GATGGAAGGG GGGAATCTAT GGTCGATGGC TCCCAGAAAG AGGAGTTCCA 
CCTGGAGTTC GTCCAGGATT TCCCAGTGAG GCTTCAGGTC ACTACCATTC CCCTGATGCT 
GGTGCTATTA GGAAAACTCT GAAAAGACTG AAGGCCGTGT CTAAGCAAGC CAGTACAAAC 
AGGCAAAAAG GACACTTAGC TGTAGAGAGA GCCAACCAAG TGGAAGAATT CCTACAGCGT 
AAACGAGAAG CTATGCAGAA TAAAGCCCGA GCCGAAGGAC ACGTGGGACT CCTGCAAAAC 
CTGGCATCTC TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAT 
GAGGAAGAGG TTTATTTGGC AAGACTGAGG CAAATAAGAC TACAAAATTT TAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAG AATAAAGAAG CTGATGGTAC CAAAGGACAA 
GAAGCAACTG AAGAGACTGA CATGAGGCTC AAAAAGATGG AGTCACTTAA GGCGCAAACA 
AATGCACGTG CTGCTGTACT AAAAGAACAG CTGGAGCGAA AAAGAAAGGA AGCTTATGAA 
AGAGAAAAGA AAGTATGGGA AGAACATTTG GTGGCGAGGG TAAAAAGCTC AGATGTTCCT 
CTGCCTTTGG AACTTCTTGA AACAGGTGGT TCTCCATCAA AGCAGCAGGT GAAGCCTGTC 
ATTTCTGTGA CTTCAGCTTT GAAAGAAGTG GGCCTGGATG GAAGTTTAAC TGATACCCAG 
GAAGAAGAAA TGGAAAAGAG TAACAGTGCT ATTTCAAGTA AGCGAGAAAT CCTGCGTAGG 
CTAAATGAAA ATCTTAAAGC TCAAGAGGAT GAAAAGGAAA AGCAGCATCA CTCAGGTTCT 
TGTGAGACCG TTGGTCACAA AGATGAGAGA GAGTATGAGA CAGAAAATGC CATTTCCTCT 
GATCGCAAGA AGTGGGAGAT GGGAGGTCAG CTTGTGATTC CTCTCGATGC AGTGACACTG 
GATACATCCT TCTCTGCAAC CGAAAAACAT ACTGTGGGAG AGGTTATTAA ATTAGATTCT 
AATGGCTCTC CAAGAAAAGT CTGGGGGAAA AACCCTACAG ATTCTGTGCT GAAGATACTT 
GGAGAAGCTG AATTACAGCT ACAGACAGAA CTACTAGAAA ACACATCTTT TAAAAGTGAG 
GTTTATGCTG AAGAGGAGAA CTACAAACCC TTACTTACTG AAGAAGAGAA TCTGCAGTGC 
ATTTCAAAAG AAATAAATCC ATCAGCTACT GTTGATTCTA CTGAAACGAA AAGTCCAAAG 
TTTACTGAGG TGTCTCCACA AATGTCAGAA GGAAATGTGG AAGAACCTGA TGATTTGGAA 
ACAGAAGTTC TACAAGAGCC AAGTAGCACA CACACAGATG GGAGTTTGCC ACCTGTTCTT 
AATGATGTGT GGACTAGAGA GAAGGAAGCA GCTAAGGAAA CTGAGTTGGA AGATAAGGTT 
GCTGTGCAGC AGAGTGAAGT TTGTGAAGAT AGAATTCCAG GGAACGTGGA CCAATCCTGT 
AAGGATCAGA GAGATCCTGC AGTAGACGAT TCTCCGCAGT CTGGCTGTGA TGTAGAGAAG 
TCAGTACAGC CAGAATCGAT TTTCCAGAAA GTGGTTCATT CTAAGGACTT GAACTTAGTT 
CAGGCAGTTC ATTGCTCACC AGAAGAACCA ATTCCAATTC GATCTCACTC TGATTCTCCA 
CCAAAAACTA AGAGCAAGAA TTCCTTACTG ATTGGACTTT CAACTGGTCT GTTTGATGCA 
AACAATCCAA AGATGCTGAG GACCTGCTCA CTTCCAGATC TTTCCAAGCT GTTCAGAACC 
CTAATGGACG TTCCCACTGT GGGGGACGTT CATCAAGACA GTCTTGAAAT CGATGAGCTG 
GAAGATGAAC CAATTAAAGA AGGGCCTTCT GATTCCGAAG ACACTGTATT TGAAGAAACT 
GACACAGATT TACAAGAGCT TCAGGCCTCA ATGGAGCAGC TGCTTAGGGA GCAACCAGGT 
GACGAATACA GTGAGGAGGA AGAGTCTGTT TTAAAAAGCA GCGATGTGGA GCAGACAGCA 
AGAGGGACAG ATGCCCCAGA CGAGGAGGAC AACCCCAGCA GCGAAAGCGC CCTGAACGAG 
GAATGGCACT CAGATAATAG TGACGCTGAG ACCACTAGTG AATGTGAATA TGACAGTGTC 
TTTAACCATT TAGAGGAACT AAGACTTCAC TTGGAGCAAG AAATGGGCTT TGAAAAGTTC 
TTTGAGGTTT ATGAGAAAGT AAAGGCTATT CATGAGGATG AAGATGAAAA TATTGAAATT 
TGTTCAACAA TAGTTGAGAA TATTTTGGGC AATGAGCACC AGCATCTCTA TGCCAAGATT 
CTGCATTTAG TCATGGCAGA TGGAGCCTAT CAGGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_030243340.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099200.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu82381
Clone ID Related Accession (Same CDS sequence)	XM_017312611.1 , XM_030243340.1
Accession Version	XM_030243340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTACT GTGAAGGAGG TGATTTGTTT AAACGAATAA ATGCTCAGAA AGGCGCTCTG 
TTTCAAGAAG ACCAGATTTT GGACTGGTTT GTGCAGATAT GTTTGGCTCT GAAGCATGTA 
CATGATAGAA AAATTCTTCA CCGAGACATA AAGTCACAGA ACATATTTCT AACCAAAGAT 
GGGACAGTGC AGCTTGGAGA TTTTGGAATT GCTCGAGTTC TTAATAGTAC TGTAGAGCTG 
GCTCGAACTT GCATAGGCAC TCCATACTAC TTGTCACCTG AAATCTGTGA AAACAAGCCT 
TATAACAATA AAAGTGACAT TTGGGCTTTG GGCTGTGTCC TTTATGAGTT GTGTACACTT 
AAACATGCAT TTGAAGCTGG AAACATGAAA AACCTGGTAC TGAAGATAAT CTCCGGATCC 
TTTCCTCCAG TGTCTCCACA TTACTCCTAT GATCTCCGCA GCTTGCTGTC TCAGTTATTT 
AAAAGAAATC CTAGGGATAG ACCATCAGTC AACTCCATAT TGGAGAAAGG TTTTATAGCT 
AAACGAATCG AAAAGTTTCT CTCCCCTCAG CTTATTGCAG AAGAATTTTG TCTAAAAACA 
CTTTCAAAGT TTGGACCACA GCCTCTCCCA GGTAAAAGAC CAGCATCAGG ACAAGGTGTC 
AGTTCTTTTG TCCCTGCTCA GAAAATCACA AAGCCTGCTG CTAAATACGG AGTGCCTTTA 
ACATATAAGA AGTATGGAGA TAAAAAGTTA CTTGAGAAAA AACCACCCCC AAAACATAAA 
CAGGCCCATC AAATTCCCGT GAAGAAAATG AATTCTGGAG AAGAAAGGAA GAAAATGTCT 
GAGGAAGCAG CAAAAAAAAG AAGGTTGGAA TTTATTGAGA AAGAAAAGAA GCAAAAGGAT 
CAGATTAGGT TCCTGAAGGC TGAGCAGATG AAGCGGCAAG AGAAGCAGCG GTTGGAGAGG 
ATAAATAGGG CCAGGGAACA AGGATGGAGG AATGTTTTAA GGGCTGGTGG AAGCGGTGAA 
GTAAAGGCTT CCTTTTTTGG CATTGGAGGG GCTGTCTCTC CATCACCGTG TTCTCCTCGA 
GGCCAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCGGCTAAG AGCAGAAGAT 
AATGAAGCAA GATGGAAGGG GGGAATCTAT GGTCGATGGC TCCCAGAAAG AGGAGTTCCA 
CCTGGAGTTC GTCCAGGATT TCCCAGTGAG GCTTCAGGTC ACTACCATTC CCCTGATGCT 
GGTGCTATTA GGAAAACTCT GAAAAGACTG AAGGCCGTGT CTAAGCAAGC CAGTACAAAC 
AGGCAAAAAG GACACTTAGC TGTAGAGAGA GCCAACCAAG TGGAAGAATT CCTACAGCGT 
AAACGAGAAG CTATGCAGAA TAAAGCCCGA GCCGAAGGAC ACGTGGGACT CCTGCAAAAC 
CTGGCATCTC TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAT 
GAGGAAGAGG TTTATTTGGC AAGACTGAGG CAAATAAGAC TACAAAATTT TAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAG AATAAAGAAG CTGATGGTAC CAAAGGACAA 
GAAGCAACTG AAGAGACTGA CATGAGGCTC AAAAAGATGG AGTCACTTAA GGCGCAAACA 
AATGCACGTG CTGCTGTACT AAAAGAACAG CTGGAGCGAA AAAGAAAGGA AGCTTATGAA 
AGAGAAAAGA AAGTATGGGA AGAACATTTG GTGGCGAGGG TAAAAAGCTC AGATGTTCCT 
CTGCCTTTGG AACTTCTTGA AACAGGTGGT TCTCCATCAA AGCAGCAGGT GAAGCCTGTC 
ATTTCTGTGA CTTCAGCTTT GAAAGAAGTG GGCCTGGATG GAAGTTTAAC TGATACCCAG 
GAAGAAGAAA TGGAAAAGAG TAACAGTGCT ATTTCAAGTA AGCGAGAAAT CCTGCGTAGG 
CTAAATGAAA ATCTTAAAGC TCAAGAGGAT GAAAAGGAAA AGCAGCATCA CTCAGGTTCT 
TGTGAGACCG TTGGTCACAA AGATGAGAGA GAGTATGAGA CAGAAAATGC CATTTCCTCT 
GATCGCAAGA AGTGGGAGAT GGGAGGTCAG CTTGTGATTC CTCTCGATGC AGTGACACTG 
GATACATCCT TCTCTGCAAC CGAAAAACAT ACTGTGGGAG AGGTTATTAA ATTAGATTCT 
AATGGCTCTC CAAGAAAAGT CTGGGGGAAA AACCCTACAG ATTCTGTGCT GAAGATACTT 
GGAGAAGCTG AATTACAGCT ACAGACAGAA CTACTAGAAA ACACATCTTT TAAAAGTGAG 
GTTTATGCTG AAGAGGAGAA CTACAAACCC TTACTTACTG AAGAAGAGAA TCTGCAGTGC 
ATTTCAAAAG AAATAAATCC ATCAGCTACT GTTGATTCTA CTGAAACGAA AAGTCCAAAG 
TTTACTGAGG TGTCTCCACA AATGTCAGAA GGAAATGTGG AAGAACCTGA TGATTTGGAA 
ACAGAAGTTC TACAAGAGCC AAGTAGCACA CACACAGATG GGAGTTTGCC ACCTGTTCTT 
AATGATGTGT GGACTAGAGA GAAGGAAGCA GCTAAGGAAA CTGAGTTGGA AGATAAGGTT 
GCTGTGCAGC AGAGTGAAGT TTGTGAAGAT AGAATTCCAG GGAACGTGGA CCAATCCTGT 
AAGGATCAGA GAGATCCTGC AGTAGACGAT TCTCCGCAGT CTGGCTGTGA TGTAGAGAAG 
TCAGTACAGC CAGAATCGAT TTTCCAGAAA GTGGTTCATT CTAAGGACTT GAACTTAGTT 
CAGGCAGTTC ATTGCTCACC AGAAGAACCA ATTCCAATTC GATCTCACTC TGATTCTCCA 
CCAAAAACTA AGAGCAAGAA TTCCTTACTG ATTGGACTTT CAACTGGTCT GTTTGATGCA 
AACAATCCAA AGATGCTGAG GACCTGCTCA CTTCCAGATC TTTCCAAGCT GTTCAGAACC 
CTAATGGACG TTCCCACTGT GGGGGACGTT CATCAAGACA GTCTTGAAAT CGATGAGCTG 
GAAGATGAAC CAATTAAAGA AGGGCCTTCT GATTCCGAAG ACACTGTATT TGAAGAAACT 
GACACAGATT TACAAGAGCT TCAGGCCTCA ATGGAGCAGC TGCTTAGGGA GCAACCAGGT 
GACGAATACA GTGAGGAGGA AGAGTCTGTT TTAAAAAGCA GCGATGTGGA GCAGACAGCA 
AGAGGGACAG ATGCCCCAGA CGAGGAGGAC AACCCCAGCA GCGAAAGCGC CCTGAACGAG 
GAATGGCACT CAGATAATAG TGACGCTGAG ACCACTAGTG AATGTGAATA TGACAGTGTC 
TTTAACCATT TAGAGGAACT AAGACTTCAC TTGGAGCAAG AAATGGGCTT TGAAAAGTTC 
TTTGAGGTTT ATGAGAAAGT AAAGGCTATT CATGAGGATG AAGATGAAAA TATTGAAATT 
TGTTCAACAA TAGTTGAGAA TATTTTGGGC AATGAGCACC AGCATCTCTA TGCCAAGATT 
CTGCATTTAG TCATGGCAGA TGGAGCCTAT CAGGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099200.1
CDS	779..4369
Translation	MDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_030243340.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243340.1

ORF Insert Sequence:

ATGGATTACT GTGAAGGAGG TGATTTGTTT AAACGAATAA ATGCTCAGAA AGGCGCTCTG 
TTTCAAGAAG ACCAGATTTT GGACTGGTTT GTGCAGATAT GTTTGGCTCT GAAGCATGTA 
CATGATAGAA AAATTCTTCA CCGAGACATA AAGTCACAGA ACATATTTCT AACCAAAGAT 
GGGACAGTGC AGCTTGGAGA TTTTGGAATT GCTCGAGTTC TTAATAGTAC TGTAGAGCTG 
GCTCGAACTT GCATAGGCAC TCCATACTAC TTGTCACCTG AAATCTGTGA AAACAAGCCT 
TATAACAATA AAAGTGACAT TTGGGCTTTG GGCTGTGTCC TTTATGAGTT GTGTACACTT 
AAACATGCAT TTGAAGCTGG AAACATGAAA AACCTGGTAC TGAAGATAAT CTCCGGATCC 
TTTCCTCCAG TGTCTCCACA TTACTCCTAT GATCTCCGCA GCTTGCTGTC TCAGTTATTT 
AAAAGAAATC CTAGGGATAG ACCATCAGTC AACTCCATAT TGGAGAAAGG TTTTATAGCT 
AAACGAATCG AAAAGTTTCT CTCCCCTCAG CTTATTGCAG AAGAATTTTG TCTAAAAACA 
CTTTCAAAGT TTGGACCACA GCCTCTCCCA GGTAAAAGAC CAGCATCAGG ACAAGGTGTC 
AGTTCTTTTG TCCCTGCTCA GAAAATCACA AAGCCTGCTG CTAAATACGG AGTGCCTTTA 
ACATATAAGA AGTATGGAGA TAAAAAGTTA CTTGAGAAAA AACCACCCCC AAAACATAAA 
CAGGCCCATC AAATTCCCGT GAAGAAAATG AATTCTGGAG AAGAAAGGAA GAAAATGTCT 
GAGGAAGCAG CAAAAAAAAG AAGGTTGGAA TTTATTGAGA AAGAAAAGAA GCAAAAGGAT 
CAGATTAGGT TCCTGAAGGC TGAGCAGATG AAGCGGCAAG AGAAGCAGCG GTTGGAGAGG 
ATAAATAGGG CCAGGGAACA AGGATGGAGG AATGTTTTAA GGGCTGGTGG AAGCGGTGAA 
GTAAAGGCTT CCTTTTTTGG CATTGGAGGG GCTGTCTCTC CATCACCGTG TTCTCCTCGA 
GGCCAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCGGCTAAG AGCAGAAGAT 
AATGAAGCAA GATGGAAGGG GGGAATCTAT GGTCGATGGC TCCCAGAAAG AGGAGTTCCA 
CCTGGAGTTC GTCCAGGATT TCCCAGTGAG GCTTCAGGTC ACTACCATTC CCCTGATGCT 
GGTGCTATTA GGAAAACTCT GAAAAGACTG AAGGCCGTGT CTAAGCAAGC CAGTACAAAC 
AGGCAAAAAG GACACTTAGC TGTAGAGAGA GCCAACCAAG TGGAAGAATT CCTACAGCGT 
AAACGAGAAG CTATGCAGAA TAAAGCCCGA GCCGAAGGAC ACGTGGGACT CCTGCAAAAC 
CTGGCATCTC TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGGAACAAT 
GAGGAAGAGG TTTATTTGGC AAGACTGAGG CAAATAAGAC TACAAAATTT TAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAG AATAAAGAAG CTGATGGTAC CAAAGGACAA 
GAAGCAACTG AAGAGACTGA CATGAGGCTC AAAAAGATGG AGTCACTTAA GGCGCAAACA 
AATGCACGTG CTGCTGTACT AAAAGAACAG CTGGAGCGAA AAAGAAAGGA AGCTTATGAA 
AGAGAAAAGA AAGTATGGGA AGAACATTTG GTGGCGAGGG TAAAAAGCTC AGATGTTCCT 
CTGCCTTTGG AACTTCTTGA AACAGGTGGT TCTCCATCAA AGCAGCAGGT GAAGCCTGTC 
ATTTCTGTGA CTTCAGCTTT GAAAGAAGTG GGCCTGGATG GAAGTTTAAC TGATACCCAG 
GAAGAAGAAA TGGAAAAGAG TAACAGTGCT ATTTCAAGTA AGCGAGAAAT CCTGCGTAGG 
CTAAATGAAA ATCTTAAAGC TCAAGAGGAT GAAAAGGAAA AGCAGCATCA CTCAGGTTCT 
TGTGAGACCG TTGGTCACAA AGATGAGAGA GAGTATGAGA CAGAAAATGC CATTTCCTCT 
GATCGCAAGA AGTGGGAGAT GGGAGGTCAG CTTGTGATTC CTCTCGATGC AGTGACACTG 
GATACATCCT TCTCTGCAAC CGAAAAACAT ACTGTGGGAG AGGTTATTAA ATTAGATTCT 
AATGGCTCTC CAAGAAAAGT CTGGGGGAAA AACCCTACAG ATTCTGTGCT GAAGATACTT 
GGAGAAGCTG AATTACAGCT ACAGACAGAA CTACTAGAAA ACACATCTTT TAAAAGTGAG 
GTTTATGCTG AAGAGGAGAA CTACAAACCC TTACTTACTG AAGAAGAGAA TCTGCAGTGC 
ATTTCAAAAG AAATAAATCC ATCAGCTACT GTTGATTCTA CTGAAACGAA AAGTCCAAAG 
TTTACTGAGG TGTCTCCACA AATGTCAGAA GGAAATGTGG AAGAACCTGA TGATTTGGAA 
ACAGAAGTTC TACAAGAGCC AAGTAGCACA CACACAGATG GGAGTTTGCC ACCTGTTCTT 
AATGATGTGT GGACTAGAGA GAAGGAAGCA GCTAAGGAAA CTGAGTTGGA AGATAAGGTT 
GCTGTGCAGC AGAGTGAAGT TTGTGAAGAT AGAATTCCAG GGAACGTGGA CCAATCCTGT 
AAGGATCAGA GAGATCCTGC AGTAGACGAT TCTCCGCAGT CTGGCTGTGA TGTAGAGAAG 
TCAGTACAGC CAGAATCGAT TTTCCAGAAA GTGGTTCATT CTAAGGACTT GAACTTAGTT 
CAGGCAGTTC ATTGCTCACC AGAAGAACCA ATTCCAATTC GATCTCACTC TGATTCTCCA 
CCAAAAACTA AGAGCAAGAA TTCCTTACTG ATTGGACTTT CAACTGGTCT GTTTGATGCA 
AACAATCCAA AGATGCTGAG GACCTGCTCA CTTCCAGATC TTTCCAAGCT GTTCAGAACC 
CTAATGGACG TTCCCACTGT GGGGGACGTT CATCAAGACA GTCTTGAAAT CGATGAGCTG 
GAAGATGAAC CAATTAAAGA AGGGCCTTCT GATTCCGAAG ACACTGTATT TGAAGAAACT 
GACACAGATT TACAAGAGCT TCAGGCCTCA ATGGAGCAGC TGCTTAGGGA GCAACCAGGT 
GACGAATACA GTGAGGAGGA AGAGTCTGTT TTAAAAAGCA GCGATGTGGA GCAGACAGCA 
AGAGGGACAG ATGCCCCAGA CGAGGAGGAC AACCCCAGCA GCGAAAGCGC CCTGAACGAG 
GAATGGCACT CAGATAATAG TGACGCTGAG ACCACTAGTG AATGTGAATA TGACAGTGTC 
TTTAACCATT TAGAGGAACT AAGACTTCAC TTGGAGCAAG AAATGGGCTT TGAAAAGTTC 
TTTGAGGTTT ATGAGAAAGT AAAGGCTATT CATGAGGATG AAGATGAAAA TATTGAAATT 
TGTTCAACAA TAGTTGAGAA TATTTTGGGC AATGAGCACC AGCATCTCTA TGCCAAGATT 
CTGCATTTAG TCATGGCAGA TGGAGCCTAT CAGGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( NM_175089.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780298.2 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 3, mRNA.

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CloneID	OMu23426
Clone ID Related Accession (Same CDS sequence)	NM_175089.4
Accession Version	NM_175089.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX237035.1, AY850065.1, AK135564.1 and BC070443.1. On Jun 11, 2014 this sequence version replaced NM_175089.3. Transcript Variant: This variant (3) lacks two alternate in-frame exons, compared to variant 1. The encoded protein (isoform 3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070443.1, AY850065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGGCA AAAAGGACAC 
TTAGCTGTAG AGAGAGCCAA CCAAGTGGAA GAATTCCTAC AGCGTAAACG AGAAGCTATG 
CAGAATAAAG CCCGAGCCGA AGGACACGTG GTTTATTTGG CAAGACTGAG GCAAATAAGA 
CTACAAAATT TTAATGAGCG CCAACAGATT AAAGCCAAAC TTCGTGGTGA GAATAAAGAA 
GCTGATGGTA CCAAAGGACA AGAAGCAACT GAAGAGACTG ACATGAGGCT CAAAAAGATG 
GAGTCACTTA AGGCGCAAAC AAATGCACGT GCTGCTGTAC TAAAAGAACA GCTGGAGCGA 
AAAAGAAAGG AAGCTTATGA AAGAGAAAAG AAAGTATGGG AAGAACATTT GGTGGCGAGG 
GTAAAAAGCT CAGATGTTCC TCTGCCTTTG GAACTTCTTG AAACAGGTGG TTCTCCATCA 
AAGCAGCAGG TGAAGCCTGT CATTTCTGTG ACTTCAGCTT TGAAAGAAGT GGGCCTGGAT 
GGAAGTTTAA CTGATACCCA GGAAGAAGAA ATGGAAAAGA GTAACAGTGC TATTTCAAGT 
AAGCGAGAAA TCCTGCGTAG GCTAAATGAA AATCTTAAAG CTCAAGAGGA TGAAAAGGAA 
AAGCAGCATC ACTCAGGTTC TTGTGAGACC GTTGGTCACA AAGATGAGAG AGAGTATGAG 
ACAGAAAATG CCATTTCCTC TGATCGCAAG AAGTGGGAGA TGGGAGGTCA GCTTGTGATT 
CCTCTCGATG CAGTGACACT GGATACATCC TTCTCTGCAA CCGAAAAACA TACTGTGGGA 
GAGGTTATTA AATTAGATTC TAATGGCTCT CCAAGAAAAG TCTGGGGGAA AAACCCTACA 
GATTCTGTGC TGAAGATACT TGGAGAAGCT GAATTACAGC TACAGACAGA ACTACTAGAA 
AACACATCTT TTAAAAGTGA GGTTTATGCT GAAGAGGAGA ACTACAAACC CTTACTTACT 
GAAGAAGAGA ATCTGCAGTG CATTTCAAAA GAAATAAATC CATCAGCTAC TGTTGATTCT 
ACTGAAACGA AAAGTCCAAA GTTTACTGAG GTGTCTCCAC AAATGTCAGA AGGAAATGTG 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTAGCAC ACACACAGAT 
GGGAGTTTGC CACCTGTTCT TAATGATGTG TGGACTAGAG AGAAGGAAGC AGCTAAGGAA 
ACTGAGTTGG AAGATAAGGT TGCTGTGCAG CAGAGTGAAG TTTGTGAAGA TAGAATTCCA 
GGGAACGTGG ACCAATCCTG TAAGGATCAG AGAGATCCTG CAGTAGACGA TTCTCCGCAG 
TCTGGCTGTG ATGTAGAGAA GTCAGTACAG CCAGAATCGA TTTTCCAGAA AGTGGTTCAT 
TCTAAGGACT TGAACTTAGT TCAGGCAGTT CATTGCTCAC CAGAAGAACC AATTCCAATT 
CGATCTCACT CTGATTCTCC ACCAAAAACT AAGAGCAAGA ATTCCTTACT GATTGGACTT 
TCAACTGGTC TGTTTGATGC AAACAATCCA AAGATGCTGA GGACCTGCTC ACTTCCAGAT 
CTTTCCAAGC TGTTCAGAAC CCTAATGGAC GTTCCCACTG TGGGGGACGT TCATCAAGAC 
AGTCTTGAAA TCGATGAGCT GGAAGATGAA CCAATTAAAG AAGGGCCTTC TGATTCCGAA 
GACACTGTAT TTGAAGAAAC TGACACAGAT TTACAAGAGC TTCAGGCCTC AATGGAGCAG 
CTGCTTAGGG AGCAACCAGG TGACGAATAC AGTGAGGAGG AAGAGTCTGT TTTAAAAAGC 
AGCGATGTGG AGCAGACAGC AAGAGGGACA GATGCCCCAG ACGAGGAGGA CAACCCCAGC 
AGCGAAAGCG CCCTGAACGA GGAATGGCAC TCAGATAATA GTGACGCTGA GACCACTAGT 
GAATGTGAAT ATGACAGTGT CTTTAACCAT TTAGAGGAAC TAAGACTTCA CTTGGAGCAA 
GAAATGGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAG TAAAGGCTAT TCATGAGGAT 
GAAGATGAAA ATATTGAAAT TTGTTCAACA ATAGTTGAGA ATATTTTGGG CAATGAGCAC 
CAGCATCTCT ATGCCAAGAT TCTGCATTTA GTCATGGCAG ATGGAGCCTA TCAGGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780298.2
CDS	603..4214
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_175089.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175089.4

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGGCA AAAAGGACAC 
TTAGCTGTAG AGAGAGCCAA CCAAGTGGAA GAATTCCTAC AGCGTAAACG AGAAGCTATG 
CAGAATAAAG CCCGAGCCGA AGGACACGTG GTTTATTTGG CAAGACTGAG GCAAATAAGA 
CTACAAAATT TTAATGAGCG CCAACAGATT AAAGCCAAAC TTCGTGGTGA GAATAAAGAA 
GCTGATGGTA CCAAAGGACA AGAAGCAACT GAAGAGACTG ACATGAGGCT CAAAAAGATG 
GAGTCACTTA AGGCGCAAAC AAATGCACGT GCTGCTGTAC TAAAAGAACA GCTGGAGCGA 
AAAAGAAAGG AAGCTTATGA AAGAGAAAAG AAAGTATGGG AAGAACATTT GGTGGCGAGG 
GTAAAAAGCT CAGATGTTCC TCTGCCTTTG GAACTTCTTG AAACAGGTGG TTCTCCATCA 
AAGCAGCAGG TGAAGCCTGT CATTTCTGTG ACTTCAGCTT TGAAAGAAGT GGGCCTGGAT 
GGAAGTTTAA CTGATACCCA GGAAGAAGAA ATGGAAAAGA GTAACAGTGC TATTTCAAGT 
AAGCGAGAAA TCCTGCGTAG GCTAAATGAA AATCTTAAAG CTCAAGAGGA TGAAAAGGAA 
AAGCAGCATC ACTCAGGTTC TTGTGAGACC GTTGGTCACA AAGATGAGAG AGAGTATGAG 
ACAGAAAATG CCATTTCCTC TGATCGCAAG AAGTGGGAGA TGGGAGGTCA GCTTGTGATT 
CCTCTCGATG CAGTGACACT GGATACATCC TTCTCTGCAA CCGAAAAACA TACTGTGGGA 
GAGGTTATTA AATTAGATTC TAATGGCTCT CCAAGAAAAG TCTGGGGGAA AAACCCTACA 
GATTCTGTGC TGAAGATACT TGGAGAAGCT GAATTACAGC TACAGACAGA ACTACTAGAA 
AACACATCTT TTAAAAGTGA GGTTTATGCT GAAGAGGAGA ACTACAAACC CTTACTTACT 
GAAGAAGAGA ATCTGCAGTG CATTTCAAAA GAAATAAATC CATCAGCTAC TGTTGATTCT 
ACTGAAACGA AAAGTCCAAA GTTTACTGAG GTGTCTCCAC AAATGTCAGA AGGAAATGTG 
GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTAGCAC ACACACAGAT 
GGGAGTTTGC CACCTGTTCT TAATGATGTG TGGACTAGAG AGAAGGAAGC AGCTAAGGAA 
ACTGAGTTGG AAGATAAGGT TGCTGTGCAG CAGAGTGAAG TTTGTGAAGA TAGAATTCCA 
GGGAACGTGG ACCAATCCTG TAAGGATCAG AGAGATCCTG CAGTAGACGA TTCTCCGCAG 
TCTGGCTGTG ATGTAGAGAA GTCAGTACAG CCAGAATCGA TTTTCCAGAA AGTGGTTCAT 
TCTAAGGACT TGAACTTAGT TCAGGCAGTT CATTGCTCAC CAGAAGAACC AATTCCAATT 
CGATCTCACT CTGATTCTCC ACCAAAAACT AAGAGCAAGA ATTCCTTACT GATTGGACTT 
TCAACTGGTC TGTTTGATGC AAACAATCCA AAGATGCTGA GGACCTGCTC ACTTCCAGAT 
CTTTCCAAGC TGTTCAGAAC CCTAATGGAC GTTCCCACTG TGGGGGACGT TCATCAAGAC 
AGTCTTGAAA TCGATGAGCT GGAAGATGAA CCAATTAAAG AAGGGCCTTC TGATTCCGAA 
GACACTGTAT TTGAAGAAAC TGACACAGAT TTACAAGAGC TTCAGGCCTC AATGGAGCAG 
CTGCTTAGGG AGCAACCAGG TGACGAATAC AGTGAGGAGG AAGAGTCTGT TTTAAAAAGC 
AGCGATGTGG AGCAGACAGC AAGAGGGACA GATGCCCCAG ACGAGGAGGA CAACCCCAGC 
AGCGAAAGCG CCCTGAACGA GGAATGGCAC TCAGATAATA GTGACGCTGA GACCACTAGT 
GAATGTGAAT ATGACAGTGT CTTTAACCAT TTAGAGGAAC TAAGACTTCA CTTGGAGCAA 
GAAATGGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAG TAAAGGCTAT TCATGAGGAT 
GAAGATGAAA ATATTGAAAT TTGTTCAACA ATAGTTGAGA ATATTTTGGG CAATGAGCAC 
CAGCATCTCT ATGCCAAGAT TCTGCATTTA GTCATGGCAG ATGGAGCCTA TCAGGAAGAT 
AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( NM_001293638.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280567.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23428
Clone ID Related Accession (Same CDS sequence)	NM_001293638.1
Accession Version	NM_001293638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX237035.1, AY850065.1, AC159302.3, AK135564.1 and BC070443.1. On Jun 11, 2014 this sequence version replaced XM_006509295.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173292.1, BC172091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGGCA AAAAGGACAC 
TTAGCTGTAG AGAGAGCCAA CCAAGTGGAA GAATTCCTAC AGCGTAAACG AGAAGCTATG 
CAGAATAAAG CCCGAGCCGA AGGACACGTG GGACTCCTGC AAAACCTGGC ATCTCTGTAT 
GGAGGCAGGC CCAGCTCTTC AAGAGGAGGG AAGCCAAGGA ACAATGAGGA AGAGGTTTAT 
TTGGCAAGAC TGAGGCAAAT AAGACTACAA AATTTTAATG AGCGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAGAATAA AGAAGCTGAT GGTACCAAAG GACAAGAAGC AACTGAAGAG 
ACTGACATGA GGCTCAAAAA GATGGAGTCA CTTAAGGCGC AAACAAATGC ACGTGCTGCT 
GTACTAAAAG AACAGCTGGA GCGAAAAAGA AAGGAAGCTT ATGAAAGAGA AAAGAAAGTA 
TGGGAAGAAC ATTTGGTGGC GAGGGTAAAA AGCTCAGATG TTCCTCTGCC TTTGGAACTT 
CTTGAAACAG GTGGTTCTCC ATCAAAGCAG CAGGTGAAGC CTGTCATTTC TGTGACTTCA 
GCTTTGAAAG AAGTGGGCCT GGATGGAAGT TTAACTGATA CCCAGGAAGA AGAAATGGAA 
AAGAGTAACA GTGCTATTTC AAGTAAGCGA GAAATCCTGC GTAGGCTAAA TGAAAATCTT 
AAAGCTCAAG AGGATGAAAA GGAAAAGCAG CATCACTCAG GTTCTTGTGA GACCGTTGGT 
CACAAAGATG AGAGAGAGTA TGAGACAGAA AATGCCATTT CCTCTGATCG CAAGAAGTGG 
GAGATGGGAG GTCAGCTTGT GATTCCTCTC GATGCAGTGA CACTGGATAC ATCCTTCTCT 
GCAACCGAAA AACATACTGT GGGAGAGGTT ATTAAATTAG ATTCTAATGG CTCTCCAAGA 
AAAGTCTGGG GGAAAAACCC TACAGATTCT GTGCTGAAGA TACTTGGAGA AGCTGAATTA 
CAGCTACAGA CAGAACTACT AGAAAACACA TCTTTTAAAA GTGAGGTTTA TGCTGAAGAG 
GAGAACTACA AACCCTTACT TACTGAAGAA GAGAATCTGC AGTGCATTTC AAAAGAAATA 
AATCCATCAG CTACTGTTGA TTCTACTGAA ACGAAAAGTC CAAAGTTTAC TGAGGTGTCT 
CCACAAATGT CAGAAGGAAA TGTGGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTA GCACACACAC AGATGGGAGT TTGCCACCTG TTCTTAATGA TGTGTGGACT 
AGAGAGAAGG AAGCAGCTAA GGAAACTGAG TTGGAAGATA AGGTTGCTGT GCAGCAGAGT 
GAAGTTTGTG AAGATAGAAT TCCAGGGAAC GTGGACCAAT CCTGTAAGGA TCAGAGAGAT 
CCTGCAGTAG ACGATTCTCC GCAGTCTGGC TGTGATGTAG AGAAGTCAGT ACAGCCAGAA 
TCGATTTTCC AGAAAGTGGT TCATTCTAAG GACTTGAACT TAGTTCAGGC AGTTCATTGC 
TCACCAGAAG AACCAATTCC AATTCGATCT CACTCTGATT CTCCACCAAA AACTAAGAGC 
AAGAATTCCT TACTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA TCCAAAGATG 
CTGAGGACCT GCTCACTTCC AGATCTTTCC AAGCTGTTCA GAACCCTAAT GGACGTTCCC 
ACTGTGGGGG ACGTTCATCA AGACAGTCTT GAAATCGATG AGCTGGAAGA TGAACCAATT 
AAAGAAGGGC CTTCTGATTC CGAAGACACT GTATTTGAAG AAACTGACAC AGATTTACAA 
GAGCTTCAGG CCTCAATGGA GCAGCTGCTT AGGGAGCAAC CAGGTGACGA ATACAGTGAG 
GAGGAAGAGT CTGTTTTAAA AAGCAGCGAT GTGGAGCAGA CAGCAAGAGG GACAGATGCC 
CCAGACGAGG AGGACAACCC CAGCAGCGAA AGCGCCCTGA ACGAGGAATG GCACTCAGAT 
AATAGTGACG CTGAGACCAC TAGTGAATGT GAATATGACA GTGTCTTTAA CCATTTAGAG 
GAACTAAGAC TTCACTTGGA GCAAGAAATG GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAGTAAAGG CTATTCATGA GGATGAAGAT GAAAATATTG AAATTTGTTC AACAATAGTT 
GAGAATATTT TGGGCAATGA GCACCAGCAT CTCTATGCCA AGATTCTGCA TTTAGTCATG 
GCAGATGGAG CCTATCAGGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280567.1
CDS	603..4298
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001293638.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293638.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGGCA AAAAGGACAC 
TTAGCTGTAG AGAGAGCCAA CCAAGTGGAA GAATTCCTAC AGCGTAAACG AGAAGCTATG 
CAGAATAAAG CCCGAGCCGA AGGACACGTG GGACTCCTGC AAAACCTGGC ATCTCTGTAT 
GGAGGCAGGC CCAGCTCTTC AAGAGGAGGG AAGCCAAGGA ACAATGAGGA AGAGGTTTAT 
TTGGCAAGAC TGAGGCAAAT AAGACTACAA AATTTTAATG AGCGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAGAATAA AGAAGCTGAT GGTACCAAAG GACAAGAAGC AACTGAAGAG 
ACTGACATGA GGCTCAAAAA GATGGAGTCA CTTAAGGCGC AAACAAATGC ACGTGCTGCT 
GTACTAAAAG AACAGCTGGA GCGAAAAAGA AAGGAAGCTT ATGAAAGAGA AAAGAAAGTA 
TGGGAAGAAC ATTTGGTGGC GAGGGTAAAA AGCTCAGATG TTCCTCTGCC TTTGGAACTT 
CTTGAAACAG GTGGTTCTCC ATCAAAGCAG CAGGTGAAGC CTGTCATTTC TGTGACTTCA 
GCTTTGAAAG AAGTGGGCCT GGATGGAAGT TTAACTGATA CCCAGGAAGA AGAAATGGAA 
AAGAGTAACA GTGCTATTTC AAGTAAGCGA GAAATCCTGC GTAGGCTAAA TGAAAATCTT 
AAAGCTCAAG AGGATGAAAA GGAAAAGCAG CATCACTCAG GTTCTTGTGA GACCGTTGGT 
CACAAAGATG AGAGAGAGTA TGAGACAGAA AATGCCATTT CCTCTGATCG CAAGAAGTGG 
GAGATGGGAG GTCAGCTTGT GATTCCTCTC GATGCAGTGA CACTGGATAC ATCCTTCTCT 
GCAACCGAAA AACATACTGT GGGAGAGGTT ATTAAATTAG ATTCTAATGG CTCTCCAAGA 
AAAGTCTGGG GGAAAAACCC TACAGATTCT GTGCTGAAGA TACTTGGAGA AGCTGAATTA 
CAGCTACAGA CAGAACTACT AGAAAACACA TCTTTTAAAA GTGAGGTTTA TGCTGAAGAG 
GAGAACTACA AACCCTTACT TACTGAAGAA GAGAATCTGC AGTGCATTTC AAAAGAAATA 
AATCCATCAG CTACTGTTGA TTCTACTGAA ACGAAAAGTC CAAAGTTTAC TGAGGTGTCT 
CCACAAATGT CAGAAGGAAA TGTGGAAGAA CCTGATGATT TGGAAACAGA AGTTCTACAA 
GAGCCAAGTA GCACACACAC AGATGGGAGT TTGCCACCTG TTCTTAATGA TGTGTGGACT 
AGAGAGAAGG AAGCAGCTAA GGAAACTGAG TTGGAAGATA AGGTTGCTGT GCAGCAGAGT 
GAAGTTTGTG AAGATAGAAT TCCAGGGAAC GTGGACCAAT CCTGTAAGGA TCAGAGAGAT 
CCTGCAGTAG ACGATTCTCC GCAGTCTGGC TGTGATGTAG AGAAGTCAGT ACAGCCAGAA 
TCGATTTTCC AGAAAGTGGT TCATTCTAAG GACTTGAACT TAGTTCAGGC AGTTCATTGC 
TCACCAGAAG AACCAATTCC AATTCGATCT CACTCTGATT CTCCACCAAA AACTAAGAGC 
AAGAATTCCT TACTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA TCCAAAGATG 
CTGAGGACCT GCTCACTTCC AGATCTTTCC AAGCTGTTCA GAACCCTAAT GGACGTTCCC 
ACTGTGGGGG ACGTTCATCA AGACAGTCTT GAAATCGATG AGCTGGAAGA TGAACCAATT 
AAAGAAGGGC CTTCTGATTC CGAAGACACT GTATTTGAAG AAACTGACAC AGATTTACAA 
GAGCTTCAGG CCTCAATGGA GCAGCTGCTT AGGGAGCAAC CAGGTGACGA ATACAGTGAG 
GAGGAAGAGT CTGTTTTAAA AAGCAGCGAT GTGGAGCAGA CAGCAAGAGG GACAGATGCC 
CCAGACGAGG AGGACAACCC CAGCAGCGAA AGCGCCCTGA ACGAGGAATG GCACTCAGAT 
AATAGTGACG CTGAGACCAC TAGTGAATGT GAATATGACA GTGTCTTTAA CCATTTAGAG 
GAACTAAGAC TTCACTTGGA GCAAGAAATG GGCTTTGAAA AGTTCTTTGA GGTTTATGAG 
AAAGTAAAGG CTATTCATGA GGATGAAGAT GAAAATATTG AAATTTGTTC AACAATAGTT 
GAGAATATTT TGGGCAATGA GCACCAGCAT CTCTATGCCA AGATTCTGCA TTTAGTCATG 
GCAGATGGAG CCTATCAGGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( NM_001293637.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280566.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23429
Clone ID Related Accession (Same CDS sequence)	NM_001293637.1 , XM_017312608.1
Accession Version	NM_001293637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX237035.1, AY850065.1, AC159302.3, AK135564.1 and BC070443.1. On Jun 11, 2014 this sequence version replaced XM_006509293.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGGACTCCT GCAAAACCTG 
GCATCTCTGT ATGGAGGCAG GCCCAGCTCT TCAAGAGGAG GGAAGCCAAG GAACAATGAG 
GAAGAGGTTT ATTTGGCAAG ACTGAGGCAA ATAAGACTAC AAAATTTTAA TGAGCGCCAA 
CAGATTAAAG CCAAACTTCG TGGTGAGAAT AAAGAAGCTG ATGGTACCAA AGGACAAGAA 
GCAACTGAAG AGACTGACAT GAGGCTCAAA AAGATGGAGT CACTTAAGGC GCAAACAAAT 
GCACGTGCTG CTGTACTAAA AGAACAGCTG GAGCGAAAAA GAAAGGAAGC TTATGAAAGA 
GAAAAGAAAG TATGGGAAGA ACATTTGGTG GCGAGGGTAA AAAGCTCAGA TGTTCCTCTG 
CCTTTGGAAC TTCTTGAAAC AGGTGGTTCT CCATCAAAGC AGCAGGTGAA GCCTGTCATT 
TCTGTGACTT CAGCTTTGAA AGAAGTGGGC CTGGATGGAA GTTTAACTGA TACCCAGGAA 
GAAGAAATGG AAAAGAGTAA CAGTGCTATT TCAAGTAAGC GAGAAATCCT GCGTAGGCTA 
AATGAAAATC TTAAAGCTCA AGAGGATGAA AAGGAAAAGC AGCATCACTC AGGTTCTTGT 
GAGACCGTTG GTCACAAAGA TGAGAGAGAG TATGAGACAG AAAATGCCAT TTCCTCTGAT 
CGCAAGAAGT GGGAGATGGG AGGTCAGCTT GTGATTCCTC TCGATGCAGT GACACTGGAT 
ACATCCTTCT CTGCAACCGA AAAACATACT GTGGGAGAGG TTATTAAATT AGATTCTAAT 
GGCTCTCCAA GAAAAGTCTG GGGGAAAAAC CCTACAGATT CTGTGCTGAA GATACTTGGA 
GAAGCTGAAT TACAGCTACA GACAGAACTA CTAGAAAACA CATCTTTTAA AAGTGAGGTT 
TATGCTGAAG AGGAGAACTA CAAACCCTTA CTTACTGAAG AAGAGAATCT GCAGTGCATT 
TCAAAAGAAA TAAATCCATC AGCTACTGTT GATTCTACTG AAACGAAAAG TCCAAAGTTT 
ACTGAGGTGT CTCCACAAAT GTCAGAAGGA AATGTGGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TAGCACACAC ACAGATGGGA GTTTGCCACC TGTTCTTAAT 
GATGTGTGGA CTAGAGAGAA GGAAGCAGCT AAGGAAACTG AGTTGGAAGA TAAGGTTGCT 
GTGCAGCAGA GTGAAGTTTG TGAAGATAGA ATTCCAGGGA ACGTGGACCA ATCCTGTAAG 
GATCAGAGAG ATCCTGCAGT AGACGATTCT CCGCAGTCTG GCTGTGATGT AGAGAAGTCA 
GTACAGCCAG AATCGATTTT CCAGAAAGTG GTTCATTCTA AGGACTTGAA CTTAGTTCAG 
GCAGTTCATT GCTCACCAGA AGAACCAATT CCAATTCGAT CTCACTCTGA TTCTCCACCA 
AAAACTAAGA GCAAGAATTC CTTACTGATT GGACTTTCAA CTGGTCTGTT TGATGCAAAC 
AATCCAAAGA TGCTGAGGAC CTGCTCACTT CCAGATCTTT CCAAGCTGTT CAGAACCCTA 
ATGGACGTTC CCACTGTGGG GGACGTTCAT CAAGACAGTC TTGAAATCGA TGAGCTGGAA 
GATGAACCAA TTAAAGAAGG GCCTTCTGAT TCCGAAGACA CTGTATTTGA AGAAACTGAC 
ACAGATTTAC AAGAGCTTCA GGCCTCAATG GAGCAGCTGC TTAGGGAGCA ACCAGGTGAC 
GAATACAGTG AGGAGGAAGA GTCTGTTTTA AAAAGCAGCG ATGTGGAGCA GACAGCAAGA 
GGGACAGATG CCCCAGACGA GGAGGACAAC CCCAGCAGCG AAAGCGCCCT GAACGAGGAA 
TGGCACTCAG ATAATAGTGA CGCTGAGACC ACTAGTGAAT GTGAATATGA CAGTGTCTTT 
AACCATTTAG AGGAACTAAG ACTTCACTTG GAGCAAGAAA TGGGCTTTGA AAAGTTCTTT 
GAGGTTTATG AGAAAGTAAA GGCTATTCAT GAGGATGAAG ATGAAAATAT TGAAATTTGT 
TCAACAATAG TTGAGAATAT TTTGGGCAAT GAGCACCAGC ATCTCTATGC CAAGATTCTG 
CATTTAGTCA TGGCAGATGG AGCCTATCAG GAAGATAATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280566.1
CDS	603..4430
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001293637.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293637.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGGACTCCT GCAAAACCTG 
GCATCTCTGT ATGGAGGCAG GCCCAGCTCT TCAAGAGGAG GGAAGCCAAG GAACAATGAG 
GAAGAGGTTT ATTTGGCAAG ACTGAGGCAA ATAAGACTAC AAAATTTTAA TGAGCGCCAA 
CAGATTAAAG CCAAACTTCG TGGTGAGAAT AAAGAAGCTG ATGGTACCAA AGGACAAGAA 
GCAACTGAAG AGACTGACAT GAGGCTCAAA AAGATGGAGT CACTTAAGGC GCAAACAAAT 
GCACGTGCTG CTGTACTAAA AGAACAGCTG GAGCGAAAAA GAAAGGAAGC TTATGAAAGA 
GAAAAGAAAG TATGGGAAGA ACATTTGGTG GCGAGGGTAA AAAGCTCAGA TGTTCCTCTG 
CCTTTGGAAC TTCTTGAAAC AGGTGGTTCT CCATCAAAGC AGCAGGTGAA GCCTGTCATT 
TCTGTGACTT CAGCTTTGAA AGAAGTGGGC CTGGATGGAA GTTTAACTGA TACCCAGGAA 
GAAGAAATGG AAAAGAGTAA CAGTGCTATT TCAAGTAAGC GAGAAATCCT GCGTAGGCTA 
AATGAAAATC TTAAAGCTCA AGAGGATGAA AAGGAAAAGC AGCATCACTC AGGTTCTTGT 
GAGACCGTTG GTCACAAAGA TGAGAGAGAG TATGAGACAG AAAATGCCAT TTCCTCTGAT 
CGCAAGAAGT GGGAGATGGG AGGTCAGCTT GTGATTCCTC TCGATGCAGT GACACTGGAT 
ACATCCTTCT CTGCAACCGA AAAACATACT GTGGGAGAGG TTATTAAATT AGATTCTAAT 
GGCTCTCCAA GAAAAGTCTG GGGGAAAAAC CCTACAGATT CTGTGCTGAA GATACTTGGA 
GAAGCTGAAT TACAGCTACA GACAGAACTA CTAGAAAACA CATCTTTTAA AAGTGAGGTT 
TATGCTGAAG AGGAGAACTA CAAACCCTTA CTTACTGAAG AAGAGAATCT GCAGTGCATT 
TCAAAAGAAA TAAATCCATC AGCTACTGTT GATTCTACTG AAACGAAAAG TCCAAAGTTT 
ACTGAGGTGT CTCCACAAAT GTCAGAAGGA AATGTGGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TAGCACACAC ACAGATGGGA GTTTGCCACC TGTTCTTAAT 
GATGTGTGGA CTAGAGAGAA GGAAGCAGCT AAGGAAACTG AGTTGGAAGA TAAGGTTGCT 
GTGCAGCAGA GTGAAGTTTG TGAAGATAGA ATTCCAGGGA ACGTGGACCA ATCCTGTAAG 
GATCAGAGAG ATCCTGCAGT AGACGATTCT CCGCAGTCTG GCTGTGATGT AGAGAAGTCA 
GTACAGCCAG AATCGATTTT CCAGAAAGTG GTTCATTCTA AGGACTTGAA CTTAGTTCAG 
GCAGTTCATT GCTCACCAGA AGAACCAATT CCAATTCGAT CTCACTCTGA TTCTCCACCA 
AAAACTAAGA GCAAGAATTC CTTACTGATT GGACTTTCAA CTGGTCTGTT TGATGCAAAC 
AATCCAAAGA TGCTGAGGAC CTGCTCACTT CCAGATCTTT CCAAGCTGTT CAGAACCCTA 
ATGGACGTTC CCACTGTGGG GGACGTTCAT CAAGACAGTC TTGAAATCGA TGAGCTGGAA 
GATGAACCAA TTAAAGAAGG GCCTTCTGAT TCCGAAGACA CTGTATTTGA AGAAACTGAC 
ACAGATTTAC AAGAGCTTCA GGCCTCAATG GAGCAGCTGC TTAGGGAGCA ACCAGGTGAC 
GAATACAGTG AGGAGGAAGA GTCTGTTTTA AAAAGCAGCG ATGTGGAGCA GACAGCAAGA 
GGGACAGATG CCCCAGACGA GGAGGACAAC CCCAGCAGCG AAAGCGCCCT GAACGAGGAA 
TGGCACTCAG ATAATAGTGA CGCTGAGACC ACTAGTGAAT GTGAATATGA CAGTGTCTTT 
AACCATTTAG AGGAACTAAG ACTTCACTTG GAGCAAGAAA TGGGCTTTGA AAAGTTCTTT 
GAGGTTTATG AGAAAGTAAA GGCTATTCAT GAGGATGAAG ATGAAAATAT TGAAATTTGT 
TCAACAATAG TTGAGAATAT TTTGGGCAAT GAGCACCAGC ATCTCTATGC CAAGATTCTG 
CATTTAGTCA TGGCAGATGG AGCCTATCAG GAAGATAATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( NM_001293639.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280568.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 4, mRNA.

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CloneID	OMu23430
Clone ID Related Accession (Same CDS sequence)	NM_001293639.1
Accession Version	NM_001293639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX237035.1, AY850065.1, AK135564.1 and BC070443.1. Transcript Variant: This variant (4) lacks three alternate in-frame exons, compared to variant 1. The encoded protein (isoform 4) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC157939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGGC TTCCTTTTTT 
GGCATTGGAG GGGCTGTCTC TCCATCACCG TGTTCTCCTC GAGGCCAGTA TGAACATTAC 
CATGCCATTT TTGACCAAAT GCAGCGGCTA AGAGCAGAAG ATAATGAAGC AAGATGGAAG 
GGGGGAATCT ATGGTCGATG GCTCCCAGAA AGGCAAAAAG GACACTTAGC TGTAGAGAGA 
GCCAACCAAG TGGAAGAATT CCTACAGCGT AAACGAGAAG CTATGCAGAA TAAAGCCCGA 
GCCGAAGGAC ACGTGGTTTA TTTGGCAAGA CTGAGGCAAA TAAGACTACA AAATTTTAAT 
GAGCGCCAAC AGATTAAAGC CAAACTTCGT GGTGAGAATA AAGAAGCTGA TGGTACCAAA 
GGACAAGAAG CAACTGAAGA GACTGACATG AGGCTCAAAA AGATGGAGTC ACTTAAGGCG 
CAAACAAATG CACGTGCTGC TGTACTAAAA GAACAGCTGG AGCGAAAAAG AAAGGAAGCT 
TATGAAAGAG AAAAGAAAGT ATGGGAAGAA CATTTGGTGG CGAGGGTAAA AAGCTCAGAT 
GTTCCTCTGC CTTTGGAACT TCTTGAAACA GGTGGTTCTC CATCAAAGCA GCAGGTGAAG 
CCTGTCATTT CTGTGACTTC AGCTTTGAAA GAAGTGGGCC TGGATGGAAG TTTAACTGAT 
ACCCAGGAAG AAGAAATGGA AAAGAGTAAC AGTGCTATTT CAAGTAAGCG AGAAATCCTG 
CGTAGGCTAA ATGAAAATCT TAAAGCTCAA GAGGATGAAA AGGAAAAGCA GCATCACTCA 
GGTTCTTGTG AGACCGTTGG TCACAAAGAT GAGAGAGAGT ATGAGACAGA AAATGCCATT 
TCCTCTGATC GCAAGAAGTG GGAGATGGGA GGTCAGCTTG TGATTCCTCT CGATGCAGTG 
ACACTGGATA CATCCTTCTC TGCAACCGAA AAACATACTG TGGGAGAGGT TATTAAATTA 
GATTCTAATG GCTCTCCAAG AAAAGTCTGG GGGAAAAACC CTACAGATTC TGTGCTGAAG 
ATACTTGGAG AAGCTGAATT ACAGCTACAG ACAGAACTAC TAGAAAACAC ATCTTTTAAA 
AGTGAGGTTT ATGCTGAAGA GGAGAACTAC AAACCCTTAC TTACTGAAGA AGAGAATCTG 
CAGTGCATTT CAAAAGAAAT AAATCCATCA GCTACTGTTG ATTCTACTGA AACGAAAAGT 
CCAAAGTTTA CTGAGGTGTC TCCACAAATG TCAGAAGGAA ATGTGGAAGA ACCTGATGAT 
TTGGAAACAG AAGTTCTACA AGAGCCAAGT AGCACACACA CAGATGGGAG TTTGCCACCT 
GTTCTTAATG ATGTGTGGAC TAGAGAGAAG GAAGCAGCTA AGGAAACTGA GTTGGAAGAT 
AAGGTTGCTG TGCAGCAGAG TGAAGTTTGT GAAGATAGAA TTCCAGGGAA CGTGGACCAA 
TCCTGTAAGG ATCAGAGAGA TCCTGCAGTA GACGATTCTC CGCAGTCTGG CTGTGATGTA 
GAGAAGTCAG TACAGCCAGA ATCGATTTTC CAGAAAGTGG TTCATTCTAA GGACTTGAAC 
TTAGTTCAGG CAGTTCATTG CTCACCAGAA GAACCAATTC CAATTCGATC TCACTCTGAT 
TCTCCACCAA AAACTAAGAG CAAGAATTCC TTACTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ATCCAAAGAT GCTGAGGACC TGCTCACTTC CAGATCTTTC CAAGCTGTTC 
AGAACCCTAA TGGACGTTCC CACTGTGGGG GACGTTCATC AAGACAGTCT TGAAATCGAT 
GAGCTGGAAG ATGAACCAAT TAAAGAAGGG CCTTCTGATT CCGAAGACAC TGTATTTGAA 
GAAACTGACA CAGATTTACA AGAGCTTCAG GCCTCAATGG AGCAGCTGCT TAGGGAGCAA 
CCAGGTGACG AATACAGTGA GGAGGAAGAG TCTGTTTTAA AAAGCAGCGA TGTGGAGCAG 
ACAGCAAGAG GGACAGATGC CCCAGACGAG GAGGACAACC CCAGCAGCGA AAGCGCCCTG 
AACGAGGAAT GGCACTCAGA TAATAGTGAC GCTGAGACCA CTAGTGAATG TGAATATGAC 
AGTGTCTTTA ACCATTTAGA GGAACTAAGA CTTCACTTGG AGCAAGAAAT GGGCTTTGAA 
AAGTTCTTTG AGGTTTATGA GAAAGTAAAG GCTATTCATG AGGATGAAGA TGAAAATATT 
GAAATTTGTT CAACAATAGT TGAGAATATT TTGGGCAATG AGCACCAGCA TCTCTATGCC 
AAGATTCTGC ATTTAGTCAT GGCAGATGGA GCCTATCAGG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280568.1
CDS	603..4139
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001293639.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293639.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGGC TTCCTTTTTT 
GGCATTGGAG GGGCTGTCTC TCCATCACCG TGTTCTCCTC GAGGCCAGTA TGAACATTAC 
CATGCCATTT TTGACCAAAT GCAGCGGCTA AGAGCAGAAG ATAATGAAGC AAGATGGAAG 
GGGGGAATCT ATGGTCGATG GCTCCCAGAA AGGCAAAAAG GACACTTAGC TGTAGAGAGA 
GCCAACCAAG TGGAAGAATT CCTACAGCGT AAACGAGAAG CTATGCAGAA TAAAGCCCGA 
GCCGAAGGAC ACGTGGTTTA TTTGGCAAGA CTGAGGCAAA TAAGACTACA AAATTTTAAT 
GAGCGCCAAC AGATTAAAGC CAAACTTCGT GGTGAGAATA AAGAAGCTGA TGGTACCAAA 
GGACAAGAAG CAACTGAAGA GACTGACATG AGGCTCAAAA AGATGGAGTC ACTTAAGGCG 
CAAACAAATG CACGTGCTGC TGTACTAAAA GAACAGCTGG AGCGAAAAAG AAAGGAAGCT 
TATGAAAGAG AAAAGAAAGT ATGGGAAGAA CATTTGGTGG CGAGGGTAAA AAGCTCAGAT 
GTTCCTCTGC CTTTGGAACT TCTTGAAACA GGTGGTTCTC CATCAAAGCA GCAGGTGAAG 
CCTGTCATTT CTGTGACTTC AGCTTTGAAA GAAGTGGGCC TGGATGGAAG TTTAACTGAT 
ACCCAGGAAG AAGAAATGGA AAAGAGTAAC AGTGCTATTT CAAGTAAGCG AGAAATCCTG 
CGTAGGCTAA ATGAAAATCT TAAAGCTCAA GAGGATGAAA AGGAAAAGCA GCATCACTCA 
GGTTCTTGTG AGACCGTTGG TCACAAAGAT GAGAGAGAGT ATGAGACAGA AAATGCCATT 
TCCTCTGATC GCAAGAAGTG GGAGATGGGA GGTCAGCTTG TGATTCCTCT CGATGCAGTG 
ACACTGGATA CATCCTTCTC TGCAACCGAA AAACATACTG TGGGAGAGGT TATTAAATTA 
GATTCTAATG GCTCTCCAAG AAAAGTCTGG GGGAAAAACC CTACAGATTC TGTGCTGAAG 
ATACTTGGAG AAGCTGAATT ACAGCTACAG ACAGAACTAC TAGAAAACAC ATCTTTTAAA 
AGTGAGGTTT ATGCTGAAGA GGAGAACTAC AAACCCTTAC TTACTGAAGA AGAGAATCTG 
CAGTGCATTT CAAAAGAAAT AAATCCATCA GCTACTGTTG ATTCTACTGA AACGAAAAGT 
CCAAAGTTTA CTGAGGTGTC TCCACAAATG TCAGAAGGAA ATGTGGAAGA ACCTGATGAT 
TTGGAAACAG AAGTTCTACA AGAGCCAAGT AGCACACACA CAGATGGGAG TTTGCCACCT 
GTTCTTAATG ATGTGTGGAC TAGAGAGAAG GAAGCAGCTA AGGAAACTGA GTTGGAAGAT 
AAGGTTGCTG TGCAGCAGAG TGAAGTTTGT GAAGATAGAA TTCCAGGGAA CGTGGACCAA 
TCCTGTAAGG ATCAGAGAGA TCCTGCAGTA GACGATTCTC CGCAGTCTGG CTGTGATGTA 
GAGAAGTCAG TACAGCCAGA ATCGATTTTC CAGAAAGTGG TTCATTCTAA GGACTTGAAC 
TTAGTTCAGG CAGTTCATTG CTCACCAGAA GAACCAATTC CAATTCGATC TCACTCTGAT 
TCTCCACCAA AAACTAAGAG CAAGAATTCC TTACTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ATCCAAAGAT GCTGAGGACC TGCTCACTTC CAGATCTTTC CAAGCTGTTC 
AGAACCCTAA TGGACGTTCC CACTGTGGGG GACGTTCATC AAGACAGTCT TGAAATCGAT 
GAGCTGGAAG ATGAACCAAT TAAAGAAGGG CCTTCTGATT CCGAAGACAC TGTATTTGAA 
GAAACTGACA CAGATTTACA AGAGCTTCAG GCCTCAATGG AGCAGCTGCT TAGGGAGCAA 
CCAGGTGACG AATACAGTGA GGAGGAAGAG TCTGTTTTAA AAAGCAGCGA TGTGGAGCAG 
ACAGCAAGAG GGACAGATGC CCCAGACGAG GAGGACAACC CCAGCAGCGA AAGCGCCCTG 
AACGAGGAAT GGCACTCAGA TAATAGTGAC GCTGAGACCA CTAGTGAATG TGAATATGAC 
AGTGTCTTTA ACCATTTAGA GGAACTAAGA CTTCACTTGG AGCAAGAAAT GGGCTTTGAA 
AAGTTCTTTG AGGTTTATGA GAAAGTAAAG GCTATTCATG AGGATGAAGA TGAAAATATT 
GAAATTTGTT CAACAATAGT TGAGAATATT TTGGGCAATG AGCACCAGCA TCTCTATGCC 
AAGATTCTGC ATTTAGTCAT GGCAGATGGA GCCTATCAGG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_017312608.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168097.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X1, mRNA.

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CloneID	OMu23429
Clone ID Related Accession (Same CDS sequence)	NM_001293637.1 , XM_017312608.1
Accession Version	XM_017312608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGGACTCCT GCAAAACCTG 
GCATCTCTGT ATGGAGGCAG GCCCAGCTCT TCAAGAGGAG GGAAGCCAAG GAACAATGAG 
GAAGAGGTTT ATTTGGCAAG ACTGAGGCAA ATAAGACTAC AAAATTTTAA TGAGCGCCAA 
CAGATTAAAG CCAAACTTCG TGGTGAGAAT AAAGAAGCTG ATGGTACCAA AGGACAAGAA 
GCAACTGAAG AGACTGACAT GAGGCTCAAA AAGATGGAGT CACTTAAGGC GCAAACAAAT 
GCACGTGCTG CTGTACTAAA AGAACAGCTG GAGCGAAAAA GAAAGGAAGC TTATGAAAGA 
GAAAAGAAAG TATGGGAAGA ACATTTGGTG GCGAGGGTAA AAAGCTCAGA TGTTCCTCTG 
CCTTTGGAAC TTCTTGAAAC AGGTGGTTCT CCATCAAAGC AGCAGGTGAA GCCTGTCATT 
TCTGTGACTT CAGCTTTGAA AGAAGTGGGC CTGGATGGAA GTTTAACTGA TACCCAGGAA 
GAAGAAATGG AAAAGAGTAA CAGTGCTATT TCAAGTAAGC GAGAAATCCT GCGTAGGCTA 
AATGAAAATC TTAAAGCTCA AGAGGATGAA AAGGAAAAGC AGCATCACTC AGGTTCTTGT 
GAGACCGTTG GTCACAAAGA TGAGAGAGAG TATGAGACAG AAAATGCCAT TTCCTCTGAT 
CGCAAGAAGT GGGAGATGGG AGGTCAGCTT GTGATTCCTC TCGATGCAGT GACACTGGAT 
ACATCCTTCT CTGCAACCGA AAAACATACT GTGGGAGAGG TTATTAAATT AGATTCTAAT 
GGCTCTCCAA GAAAAGTCTG GGGGAAAAAC CCTACAGATT CTGTGCTGAA GATACTTGGA 
GAAGCTGAAT TACAGCTACA GACAGAACTA CTAGAAAACA CATCTTTTAA AAGTGAGGTT 
TATGCTGAAG AGGAGAACTA CAAACCCTTA CTTACTGAAG AAGAGAATCT GCAGTGCATT 
TCAAAAGAAA TAAATCCATC AGCTACTGTT GATTCTACTG AAACGAAAAG TCCAAAGTTT 
ACTGAGGTGT CTCCACAAAT GTCAGAAGGA AATGTGGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TAGCACACAC ACAGATGGGA GTTTGCCACC TGTTCTTAAT 
GATGTGTGGA CTAGAGAGAA GGAAGCAGCT AAGGAAACTG AGTTGGAAGA TAAGGTTGCT 
GTGCAGCAGA GTGAAGTTTG TGAAGATAGA ATTCCAGGGA ACGTGGACCA ATCCTGTAAG 
GATCAGAGAG ATCCTGCAGT AGACGATTCT CCGCAGTCTG GCTGTGATGT AGAGAAGTCA 
GTACAGCCAG AATCGATTTT CCAGAAAGTG GTTCATTCTA AGGACTTGAA CTTAGTTCAG 
GCAGTTCATT GCTCACCAGA AGAACCAATT CCAATTCGAT CTCACTCTGA TTCTCCACCA 
AAAACTAAGA GCAAGAATTC CTTACTGATT GGACTTTCAA CTGGTCTGTT TGATGCAAAC 
AATCCAAAGA TGCTGAGGAC CTGCTCACTT CCAGATCTTT CCAAGCTGTT CAGAACCCTA 
ATGGACGTTC CCACTGTGGG GGACGTTCAT CAAGACAGTC TTGAAATCGA TGAGCTGGAA 
GATGAACCAA TTAAAGAAGG GCCTTCTGAT TCCGAAGACA CTGTATTTGA AGAAACTGAC 
ACAGATTTAC AAGAGCTTCA GGCCTCAATG GAGCAGCTGC TTAGGGAGCA ACCAGGTGAC 
GAATACAGTG AGGAGGAAGA GTCTGTTTTA AAAAGCAGCG ATGTGGAGCA GACAGCAAGA 
GGGACAGATG CCCCAGACGA GGAGGACAAC CCCAGCAGCG AAAGCGCCCT GAACGAGGAA 
TGGCACTCAG ATAATAGTGA CGCTGAGACC ACTAGTGAAT GTGAATATGA CAGTGTCTTT 
AACCATTTAG AGGAACTAAG ACTTCACTTG GAGCAAGAAA TGGGCTTTGA AAAGTTCTTT 
GAGGTTTATG AGAAAGTAAA GGCTATTCAT GAGGATGAAG ATGAAAATAT TGAAATTTGT 
TCAACAATAG TTGAGAATAT TTTGGGCAAT GAGCACCAGC ATCTCTATGC CAAGATTCTG 
CATTTAGTCA TGGCAGATGG AGCCTATCAG GAAGATAATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168097.1
CDS	50..3877
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017312608.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312608.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGGACTCCT GCAAAACCTG 
GCATCTCTGT ATGGAGGCAG GCCCAGCTCT TCAAGAGGAG GGAAGCCAAG GAACAATGAG 
GAAGAGGTTT ATTTGGCAAG ACTGAGGCAA ATAAGACTAC AAAATTTTAA TGAGCGCCAA 
CAGATTAAAG CCAAACTTCG TGGTGAGAAT AAAGAAGCTG ATGGTACCAA AGGACAAGAA 
GCAACTGAAG AGACTGACAT GAGGCTCAAA AAGATGGAGT CACTTAAGGC GCAAACAAAT 
GCACGTGCTG CTGTACTAAA AGAACAGCTG GAGCGAAAAA GAAAGGAAGC TTATGAAAGA 
GAAAAGAAAG TATGGGAAGA ACATTTGGTG GCGAGGGTAA AAAGCTCAGA TGTTCCTCTG 
CCTTTGGAAC TTCTTGAAAC AGGTGGTTCT CCATCAAAGC AGCAGGTGAA GCCTGTCATT 
TCTGTGACTT CAGCTTTGAA AGAAGTGGGC CTGGATGGAA GTTTAACTGA TACCCAGGAA 
GAAGAAATGG AAAAGAGTAA CAGTGCTATT TCAAGTAAGC GAGAAATCCT GCGTAGGCTA 
AATGAAAATC TTAAAGCTCA AGAGGATGAA AAGGAAAAGC AGCATCACTC AGGTTCTTGT 
GAGACCGTTG GTCACAAAGA TGAGAGAGAG TATGAGACAG AAAATGCCAT TTCCTCTGAT 
CGCAAGAAGT GGGAGATGGG AGGTCAGCTT GTGATTCCTC TCGATGCAGT GACACTGGAT 
ACATCCTTCT CTGCAACCGA AAAACATACT GTGGGAGAGG TTATTAAATT AGATTCTAAT 
GGCTCTCCAA GAAAAGTCTG GGGGAAAAAC CCTACAGATT CTGTGCTGAA GATACTTGGA 
GAAGCTGAAT TACAGCTACA GACAGAACTA CTAGAAAACA CATCTTTTAA AAGTGAGGTT 
TATGCTGAAG AGGAGAACTA CAAACCCTTA CTTACTGAAG AAGAGAATCT GCAGTGCATT 
TCAAAAGAAA TAAATCCATC AGCTACTGTT GATTCTACTG AAACGAAAAG TCCAAAGTTT 
ACTGAGGTGT CTCCACAAAT GTCAGAAGGA AATGTGGAAG AACCTGATGA TTTGGAAACA 
GAAGTTCTAC AAGAGCCAAG TAGCACACAC ACAGATGGGA GTTTGCCACC TGTTCTTAAT 
GATGTGTGGA CTAGAGAGAA GGAAGCAGCT AAGGAAACTG AGTTGGAAGA TAAGGTTGCT 
GTGCAGCAGA GTGAAGTTTG TGAAGATAGA ATTCCAGGGA ACGTGGACCA ATCCTGTAAG 
GATCAGAGAG ATCCTGCAGT AGACGATTCT CCGCAGTCTG GCTGTGATGT AGAGAAGTCA 
GTACAGCCAG AATCGATTTT CCAGAAAGTG GTTCATTCTA AGGACTTGAA CTTAGTTCAG 
GCAGTTCATT GCTCACCAGA AGAACCAATT CCAATTCGAT CTCACTCTGA TTCTCCACCA 
AAAACTAAGA GCAAGAATTC CTTACTGATT GGACTTTCAA CTGGTCTGTT TGATGCAAAC 
AATCCAAAGA TGCTGAGGAC CTGCTCACTT CCAGATCTTT CCAAGCTGTT CAGAACCCTA 
ATGGACGTTC CCACTGTGGG GGACGTTCAT CAAGACAGTC TTGAAATCGA TGAGCTGGAA 
GATGAACCAA TTAAAGAAGG GCCTTCTGAT TCCGAAGACA CTGTATTTGA AGAAACTGAC 
ACAGATTTAC AAGAGCTTCA GGCCTCAATG GAGCAGCTGC TTAGGGAGCA ACCAGGTGAC 
GAATACAGTG AGGAGGAAGA GTCTGTTTTA AAAAGCAGCG ATGTGGAGCA GACAGCAAGA 
GGGACAGATG CCCCAGACGA GGAGGACAAC CCCAGCAGCG AAAGCGCCCT GAACGAGGAA 
TGGCACTCAG ATAATAGTGA CGCTGAGACC ACTAGTGAAT GTGAATATGA CAGTGTCTTT 
AACCATTTAG AGGAACTAAG ACTTCACTTG GAGCAAGAAA TGGGCTTTGA AAAGTTCTTT 
GAGGTTTATG AGAAAGTAAA GGCTATTCAT GAGGATGAAG ATGAAAATAT TGAAATTTGT 
TCAACAATAG TTGAGAATAT TTTGGGCAAT GAGCACCAGC ATCTCTATGC CAAGATTCTG 
CATTTAGTCA TGGCAGATGG AGCCTATCAG GAAGATAATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_006509294.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509357.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X3, mRNA.

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CloneID	OMu40752
Clone ID Related Accession (Same CDS sequence)	XM_006509294.2 , XM_006509294.3
Accession Version	XM_006509294.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGTTTATTT GGCAAGACTG 
AGGCAAATAA GACTACAAAA TTTTAATGAG CGCCAACAGA TTAAAGCCAA ACTTCGTGGT 
GAGAATAAAG AAGCTGATGG TACCAAAGGA CAAGAAGCAA CTGAAGAGAC TGACATGAGG 
CTCAAAAAGA TGGAGTCACT TAAGGCGCAA ACAAATGCAC GTGCTGCTGT ACTAAAAGAA 
CAGCTGGAGC GAAAAAGAAA GGAAGCTTAT GAAAGAGAAA AGAAAGTATG GGAAGAACAT 
TTGGTGGCGA GGGTAAAAAG CTCAGATGTT CCTCTGCCTT TGGAACTTCT TGAAACAGGT 
GGTTCTCCAT CAAAGCAGCA GGTGAAGCCT GTCATTTCTG TGACTTCAGC TTTGAAAGAA 
GTGGGCCTGG ATGGAAGTTT AACTGATACC CAGGAAGAAG AAATGGAAAA GAGTAACAGT 
GCTATTTCAA GTAAGCGAGA AATCCTGCGT AGGCTAAATG AAAATCTTAA AGCTCAAGAG 
GATGAAAAGG AAAAGCAGCA TCACTCAGGT TCTTGTGAGA CCGTTGGTCA CAAAGATGAG 
AGAGAGTATG AGACAGAAAA TGCCATTTCC TCTGATCGCA AGAAGTGGGA GATGGGAGGT 
CAGCTTGTGA TTCCTCTCGA TGCAGTGACA CTGGATACAT CCTTCTCTGC AACCGAAAAA 
CATACTGTGG GAGAGGTTAT TAAATTAGAT TCTAATGGCT CTCCAAGAAA AGTCTGGGGG 
AAAAACCCTA CAGATTCTGT GCTGAAGATA CTTGGAGAAG CTGAATTACA GCTACAGACA 
GAACTACTAG AAAACACATC TTTTAAAAGT GAGGTTTATG CTGAAGAGGA GAACTACAAA 
CCCTTACTTA CTGAAGAAGA GAATCTGCAG TGCATTTCAA AAGAAATAAA TCCATCAGCT 
ACTGTTGATT CTACTGAAAC GAAAAGTCCA AAGTTTACTG AGGTGTCTCC ACAAATGTCA 
GAAGGAAATG TGGAAGAACC TGATGATTTG GAAACAGAAG TTCTACAAGA GCCAAGTAGC 
ACACACACAG ATGGGAGTTT GCCACCTGTT CTTAATGATG TGTGGACTAG AGAGAAGGAA 
GCAGCTAAGG AAACTGAGTT GGAAGATAAG GTTGCTGTGC AGCAGAGTGA AGTTTGTGAA 
GATAGAATTC CAGGGAACGT GGACCAATCC TGTAAGGATC AGAGAGATCC TGCAGTAGAC 
GATTCTCCGC AGTCTGGCTG TGATGTAGAG AAGTCAGTAC AGCCAGAATC GATTTTCCAG 
AAAGTGGTTC ATTCTAAGGA CTTGAACTTA GTTCAGGCAG TTCATTGCTC ACCAGAAGAA 
CCAATTCCAA TTCGATCTCA CTCTGATTCT CCACCAAAAA CTAAGAGCAA GAATTCCTTA 
CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAATC CAAAGATGCT GAGGACCTGC 
TCACTTCCAG ATCTTTCCAA GCTGTTCAGA ACCCTAATGG ACGTTCCCAC TGTGGGGGAC 
GTTCATCAAG ACAGTCTTGA AATCGATGAG CTGGAAGATG AACCAATTAA AGAAGGGCCT 
TCTGATTCCG AAGACACTGT ATTTGAAGAA ACTGACACAG ATTTACAAGA GCTTCAGGCC 
TCAATGGAGC AGCTGCTTAG GGAGCAACCA GGTGACGAAT ACAGTGAGGA GGAAGAGTCT 
GTTTTAAAAA GCAGCGATGT GGAGCAGACA GCAAGAGGGA CAGATGCCCC AGACGAGGAG 
GACAACCCCA GCAGCGAAAG CGCCCTGAAC GAGGAATGGC ACTCAGATAA TAGTGACGCT 
GAGACCACTA GTGAATGTGA ATATGACAGT GTCTTTAACC ATTTAGAGGA ACTAAGACTT 
CACTTGGAGC AAGAAATGGG CTTTGAAAAG TTCTTTGAGG TTTATGAGAA AGTAAAGGCT 
ATTCATGAGG ATGAAGATGA AAATATTGAA ATTTGTTCAA CAATAGTTGA GAATATTTTG 
GGCAATGAGC ACCAGCATCT CTATGCCAAG ATTCTGCATT TAGTCATGGC AGATGGAGCC 
TATCAGGAAG ATAATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509357.1
CDS	231..3974
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEVKASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_006509294.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509294.2

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 
AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 
AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 
CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 
GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGAGG AGTTCCACCT 
GGAGTTCGTC CAGGATTTCC CAGTGAGGCT TCAGGTCACT ACCATTCCCC TGATGCTGGT 
GCTATTAGGA AAACTCTGAA AAGACTGAAG GCCGTGTCTA AGCAAGCCAG TACAAACAGG 
CAAAAAGGAC ACTTAGCTGT AGAGAGAGCC AACCAAGTGG AAGAATTCCT ACAGCGTAAA 
CGAGAAGCTA TGCAGAATAA AGCCCGAGCC GAAGGACACG TGGTTTATTT GGCAAGACTG 
AGGCAAATAA GACTACAAAA TTTTAATGAG CGCCAACAGA TTAAAGCCAA ACTTCGTGGT 
GAGAATAAAG AAGCTGATGG TACCAAAGGA CAAGAAGCAA CTGAAGAGAC TGACATGAGG 
CTCAAAAAGA TGGAGTCACT TAAGGCGCAA ACAAATGCAC GTGCTGCTGT ACTAAAAGAA 
CAGCTGGAGC GAAAAAGAAA GGAAGCTTAT GAAAGAGAAA AGAAAGTATG GGAAGAACAT 
TTGGTGGCGA GGGTAAAAAG CTCAGATGTT CCTCTGCCTT TGGAACTTCT TGAAACAGGT 
GGTTCTCCAT CAAAGCAGCA GGTGAAGCCT GTCATTTCTG TGACTTCAGC TTTGAAAGAA 
GTGGGCCTGG ATGGAAGTTT AACTGATACC CAGGAAGAAG AAATGGAAAA GAGTAACAGT 
GCTATTTCAA GTAAGCGAGA AATCCTGCGT AGGCTAAATG AAAATCTTAA AGCTCAAGAG 
GATGAAAAGG AAAAGCAGCA TCACTCAGGT TCTTGTGAGA CCGTTGGTCA CAAAGATGAG 
AGAGAGTATG AGACAGAAAA TGCCATTTCC TCTGATCGCA AGAAGTGGGA GATGGGAGGT 
CAGCTTGTGA TTCCTCTCGA TGCAGTGACA CTGGATACAT CCTTCTCTGC AACCGAAAAA 
CATACTGTGG GAGAGGTTAT TAAATTAGAT TCTAATGGCT CTCCAAGAAA AGTCTGGGGG 
AAAAACCCTA CAGATTCTGT GCTGAAGATA CTTGGAGAAG CTGAATTACA GCTACAGACA 
GAACTACTAG AAAACACATC TTTTAAAAGT GAGGTTTATG CTGAAGAGGA GAACTACAAA 
CCCTTACTTA CTGAAGAAGA GAATCTGCAG TGCATTTCAA AAGAAATAAA TCCATCAGCT 
ACTGTTGATT CTACTGAAAC GAAAAGTCCA AAGTTTACTG AGGTGTCTCC ACAAATGTCA 
GAAGGAAATG TGGAAGAACC TGATGATTTG GAAACAGAAG TTCTACAAGA GCCAAGTAGC 
ACACACACAG ATGGGAGTTT GCCACCTGTT CTTAATGATG TGTGGACTAG AGAGAAGGAA 
GCAGCTAAGG AAACTGAGTT GGAAGATAAG GTTGCTGTGC AGCAGAGTGA AGTTTGTGAA 
GATAGAATTC CAGGGAACGT GGACCAATCC TGTAAGGATC AGAGAGATCC TGCAGTAGAC 
GATTCTCCGC AGTCTGGCTG TGATGTAGAG AAGTCAGTAC AGCCAGAATC GATTTTCCAG 
AAAGTGGTTC ATTCTAAGGA CTTGAACTTA GTTCAGGCAG TTCATTGCTC ACCAGAAGAA 
CCAATTCCAA TTCGATCTCA CTCTGATTCT CCACCAAAAA CTAAGAGCAA GAATTCCTTA 
CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAATC CAAAGATGCT GAGGACCTGC 
TCACTTCCAG ATCTTTCCAA GCTGTTCAGA ACCCTAATGG ACGTTCCCAC TGTGGGGGAC 
GTTCATCAAG ACAGTCTTGA AATCGATGAG CTGGAAGATG AACCAATTAA AGAAGGGCCT 
TCTGATTCCG AAGACACTGT ATTTGAAGAA ACTGACACAG ATTTACAAGA GCTTCAGGCC 
TCAATGGAGC AGCTGCTTAG GGAGCAACCA GGTGACGAAT ACAGTGAGGA GGAAGAGTCT 
GTTTTAAAAA GCAGCGATGT GGAGCAGACA GCAAGAGGGA CAGATGCCCC AGACGAGGAG 
GACAACCCCA GCAGCGAAAG CGCCCTGAAC GAGGAATGGC ACTCAGATAA TAGTGACGCT 
GAGACCACTA GTGAATGTGA ATATGACAGT GTCTTTAACC ATTTAGAGGA ACTAAGACTT 
CACTTGGAGC AAGAAATGGG CTTTGAAAAG TTCTTTGAGG TTTATGAGAA AGTAAAGGCT 
ATTCATGAGG ATGAAGATGA AAATATTGAA ATTTGTTCAA CAATAGTTGA GAATATTTTG 
GGCAATGAGC ACCAGCATCT CTATGCCAAG ATTCTGCATT TAGTCATGGC AGATGGAGCC 
TATCAGGAAG ATAATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_017312610.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168099.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X4, mRNA.

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CloneID	OMu40753
Clone ID Related Accession (Same CDS sequence)	XM_030243339.1 , XM_017312610.1
Accession Version	XM_017312610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GCTTCCTTTT TTGGCATTGG AGGGGCTGTC TCTCCATCAC CGTGTTCTCC TCGAGGCCAG 
TATGAACATT ACCATGCCAT TTTTGACCAA ATGCAGCGGC TAAGAGCAGA AGATAATGAA 
GCAAGATGGA AGGGGGGAAT CTATGGTCGA TGGCTCCCAG AAAGAGGAGT TCCACCTGGA 
GTTCGTCCAG GATTTCCCAG TGAGGCTTCA GGTCACTACC ATTCCCCTGA TGCTGGTGCT 
ATTAGGAAAA CTCTGAAAAG ACTGAAGGCC GTGTCTAAGC AAGCCAGTAC AAACAGGCAA 
AAAGGACACT TAGCTGTAGA GAGAGCCAAC CAAGTGGAAG AATTCCTACA GCGTAAACGA 
GAAGCTATGC AGAATAAAGC CCGAGCCGAA GGACACGTGG GACTCCTGCA AAACCTGGCA 
TCTCTGTATG GAGGCAGGCC CAGCTCTTCA AGAGGAGGGA AGCCAAGGAA CAATGAGGAA 
GAGGTTTATT TGGCAAGACT GAGGCAAATA AGACTACAAA ATTTTAATGA GCGCCAACAG 
ATTAAAGCCA AACTTCGTGG TGAGAATAAA GAAGCTGATG GTACCAAAGG ACAAGAAGCA 
ACTGAAGAGA CTGACATGAG GCTCAAAAAG ATGGAGTCAC TTAAGGCGCA AACAAATGCA 
CGTGCTGCTG TACTAAAAGA ACAGCTGGAG CGAAAAAGAA AGGAAGCTTA TGAAAGAGAA 
AAGAAAGTAT GGGAAGAACA TTTGGTGGCG AGGGTAAAAA GCTCAGATGT TCCTCTGCCT 
TTGGAACTTC TTGAAACAGG TGGTTCTCCA TCAAAGCAGC AGGTGAAGCC TGTCATTTCT 
GTGACTTCAG CTTTGAAAGA AGTGGGCCTG GATGGAAGTT TAACTGATAC CCAGGAAGAA 
GAAATGGAAA AGAGTAACAG TGCTATTTCA AGTAAGCGAG AAATCCTGCG TAGGCTAAAT 
GAAAATCTTA AAGCTCAAGA GGATGAAAAG GAAAAGCAGC ATCACTCAGG TTCTTGTGAG 
ACCGTTGGTC ACAAAGATGA GAGAGAGTAT GAGACAGAAA ATGCCATTTC CTCTGATCGC 
AAGAAGTGGG AGATGGGAGG TCAGCTTGTG ATTCCTCTCG ATGCAGTGAC ACTGGATACA 
TCCTTCTCTG CAACCGAAAA ACATACTGTG GGAGAGGTTA TTAAATTAGA TTCTAATGGC 
TCTCCAAGAA AAGTCTGGGG GAAAAACCCT ACAGATTCTG TGCTGAAGAT ACTTGGAGAA 
GCTGAATTAC AGCTACAGAC AGAACTACTA GAAAACACAT CTTTTAAAAG TGAGGTTTAT 
GCTGAAGAGG AGAACTACAA ACCCTTACTT ACTGAAGAAG AGAATCTGCA GTGCATTTCA 
AAAGAAATAA ATCCATCAGC TACTGTTGAT TCTACTGAAA CGAAAAGTCC AAAGTTTACT 
GAGGTGTCTC CACAAATGTC AGAAGGAAAT GTGGAAGAAC CTGATGATTT GGAAACAGAA 
GTTCTACAAG AGCCAAGTAG CACACACACA GATGGGAGTT TGCCACCTGT TCTTAATGAT 
GTGTGGACTA GAGAGAAGGA AGCAGCTAAG GAAACTGAGT TGGAAGATAA GGTTGCTGTG 
CAGCAGAGTG AAGTTTGTGA AGATAGAATT CCAGGGAACG TGGACCAATC CTGTAAGGAT 
CAGAGAGATC CTGCAGTAGA CGATTCTCCG CAGTCTGGCT GTGATGTAGA GAAGTCAGTA 
CAGCCAGAAT CGATTTTCCA GAAAGTGGTT CATTCTAAGG ACTTGAACTT AGTTCAGGCA 
GTTCATTGCT CACCAGAAGA ACCAATTCCA ATTCGATCTC ACTCTGATTC TCCACCAAAA 
ACTAAGAGCA AGAATTCCTT ACTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAT 
CCAAAGATGC TGAGGACCTG CTCACTTCCA GATCTTTCCA AGCTGTTCAG AACCCTAATG 
GACGTTCCCA CTGTGGGGGA CGTTCATCAA GACAGTCTTG AAATCGATGA GCTGGAAGAT 
GAACCAATTA AAGAAGGGCC TTCTGATTCC GAAGACACTG TATTTGAAGA AACTGACACA 
GATTTACAAG AGCTTCAGGC CTCAATGGAG CAGCTGCTTA GGGAGCAACC AGGTGACGAA 
TACAGTGAGG AGGAAGAGTC TGTTTTAAAA AGCAGCGATG TGGAGCAGAC AGCAAGAGGG 
ACAGATGCCC CAGACGAGGA GGACAACCCC AGCAGCGAAA GCGCCCTGAA CGAGGAATGG 
CACTCAGATA ATAGTGACGC TGAGACCACT AGTGAATGTG AATATGACAG TGTCTTTAAC 
CATTTAGAGG AACTAAGACT TCACTTGGAG CAAGAAATGG GCTTTGAAAA GTTCTTTGAG 
GTTTATGAGA AAGTAAAGGC TATTCATGAG GATGAAGATG AAAATATTGA AATTTGTTCA 
ACAATAGTTG AGAATATTTT GGGCAATGAG CACCAGCATC TCTATGCCAA GATTCTGCAT 
TTAGTCATGG CAGATGGAGC CTATCAGGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168099.1
CDS	32..3676
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017312610.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312610.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GCTTCCTTTT TTGGCATTGG AGGGGCTGTC TCTCCATCAC CGTGTTCTCC TCGAGGCCAG 
TATGAACATT ACCATGCCAT TTTTGACCAA ATGCAGCGGC TAAGAGCAGA AGATAATGAA 
GCAAGATGGA AGGGGGGAAT CTATGGTCGA TGGCTCCCAG AAAGAGGAGT TCCACCTGGA 
GTTCGTCCAG GATTTCCCAG TGAGGCTTCA GGTCACTACC ATTCCCCTGA TGCTGGTGCT 
ATTAGGAAAA CTCTGAAAAG ACTGAAGGCC GTGTCTAAGC AAGCCAGTAC AAACAGGCAA 
AAAGGACACT TAGCTGTAGA GAGAGCCAAC CAAGTGGAAG AATTCCTACA GCGTAAACGA 
GAAGCTATGC AGAATAAAGC CCGAGCCGAA GGACACGTGG GACTCCTGCA AAACCTGGCA 
TCTCTGTATG GAGGCAGGCC CAGCTCTTCA AGAGGAGGGA AGCCAAGGAA CAATGAGGAA 
GAGGTTTATT TGGCAAGACT GAGGCAAATA AGACTACAAA ATTTTAATGA GCGCCAACAG 
ATTAAAGCCA AACTTCGTGG TGAGAATAAA GAAGCTGATG GTACCAAAGG ACAAGAAGCA 
ACTGAAGAGA CTGACATGAG GCTCAAAAAG ATGGAGTCAC TTAAGGCGCA AACAAATGCA 
CGTGCTGCTG TACTAAAAGA ACAGCTGGAG CGAAAAAGAA AGGAAGCTTA TGAAAGAGAA 
AAGAAAGTAT GGGAAGAACA TTTGGTGGCG AGGGTAAAAA GCTCAGATGT TCCTCTGCCT 
TTGGAACTTC TTGAAACAGG TGGTTCTCCA TCAAAGCAGC AGGTGAAGCC TGTCATTTCT 
GTGACTTCAG CTTTGAAAGA AGTGGGCCTG GATGGAAGTT TAACTGATAC CCAGGAAGAA 
GAAATGGAAA AGAGTAACAG TGCTATTTCA AGTAAGCGAG AAATCCTGCG TAGGCTAAAT 
GAAAATCTTA AAGCTCAAGA GGATGAAAAG GAAAAGCAGC ATCACTCAGG TTCTTGTGAG 
ACCGTTGGTC ACAAAGATGA GAGAGAGTAT GAGACAGAAA ATGCCATTTC CTCTGATCGC 
AAGAAGTGGG AGATGGGAGG TCAGCTTGTG ATTCCTCTCG ATGCAGTGAC ACTGGATACA 
TCCTTCTCTG CAACCGAAAA ACATACTGTG GGAGAGGTTA TTAAATTAGA TTCTAATGGC 
TCTCCAAGAA AAGTCTGGGG GAAAAACCCT ACAGATTCTG TGCTGAAGAT ACTTGGAGAA 
GCTGAATTAC AGCTACAGAC AGAACTACTA GAAAACACAT CTTTTAAAAG TGAGGTTTAT 
GCTGAAGAGG AGAACTACAA ACCCTTACTT ACTGAAGAAG AGAATCTGCA GTGCATTTCA 
AAAGAAATAA ATCCATCAGC TACTGTTGAT TCTACTGAAA CGAAAAGTCC AAAGTTTACT 
GAGGTGTCTC CACAAATGTC AGAAGGAAAT GTGGAAGAAC CTGATGATTT GGAAACAGAA 
GTTCTACAAG AGCCAAGTAG CACACACACA GATGGGAGTT TGCCACCTGT TCTTAATGAT 
GTGTGGACTA GAGAGAAGGA AGCAGCTAAG GAAACTGAGT TGGAAGATAA GGTTGCTGTG 
CAGCAGAGTG AAGTTTGTGA AGATAGAATT CCAGGGAACG TGGACCAATC CTGTAAGGAT 
CAGAGAGATC CTGCAGTAGA CGATTCTCCG CAGTCTGGCT GTGATGTAGA GAAGTCAGTA 
CAGCCAGAAT CGATTTTCCA GAAAGTGGTT CATTCTAAGG ACTTGAACTT AGTTCAGGCA 
GTTCATTGCT CACCAGAAGA ACCAATTCCA ATTCGATCTC ACTCTGATTC TCCACCAAAA 
ACTAAGAGCA AGAATTCCTT ACTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAT 
CCAAAGATGC TGAGGACCTG CTCACTTCCA GATCTTTCCA AGCTGTTCAG AACCCTAATG 
GACGTTCCCA CTGTGGGGGA CGTTCATCAA GACAGTCTTG AAATCGATGA GCTGGAAGAT 
GAACCAATTA AAGAAGGGCC TTCTGATTCC GAAGACACTG TATTTGAAGA AACTGACACA 
GATTTACAAG AGCTTCAGGC CTCAATGGAG CAGCTGCTTA GGGAGCAACC AGGTGACGAA 
TACAGTGAGG AGGAAGAGTC TGTTTTAAAA AGCAGCGATG TGGAGCAGAC AGCAAGAGGG 
ACAGATGCCC CAGACGAGGA GGACAACCCC AGCAGCGAAA GCGCCCTGAA CGAGGAATGG 
CACTCAGATA ATAGTGACGC TGAGACCACT AGTGAATGTG AATATGACAG TGTCTTTAAC 
CATTTAGAGG AACTAAGACT TCACTTGGAG CAAGAAATGG GCTTTGAAAA GTTCTTTGAG 
GTTTATGAGA AAGTAAAGGC TATTCATGAG GATGAAGATG AAAATATTGA AATTTGTTCA 
ACAATAGTTG AGAATATTTT GGGCAATGAG CACCAGCATC TCTATGCCAA GATTCTGCAT 
TTAGTCATGG CAGATGGAGC CTATCAGGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek1 ( XM_017312609.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168098.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68310
Clone ID Related Accession (Same CDS sequence)	XM_030243338.1 , XM_017312609.1
Accession Version	XM_017312609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGGC TTCCTTTTTT 
GGCATTGGAG GGGCTGTCTC TCCATCACCG TGTTCTCCTC GAGGCCAGTA TGAACATTAC 
CATGCCATTT TTGACCAAAT GCAGCGGCTA AGAGCAGAAG ATAATGAAGC AAGATGGAAG 
GGGGGAATCT ATGGTCGATG GCTCCCAGAA AGAGGAGTTC CACCTGGAGT TCGTCCAGGA 
TTTCCCAGTG AGGCTTCAGG TCACTACCAT TCCCCTGATG CTGGTGCTAT TAGGAAAACT 
CTGAAAAGAC TGAAGGCCGT GTCTAAGCAA GCCAGTACAA ACAGGCAAAA AGGACACTTA 
GCTGTAGAGA GAGCCAACCA AGTGGAAGAA TTCCTACAGC GTAAACGAGA AGCTATGCAG 
AATAAAGCCC GAGCCGAAGG ACACGTGGGA CTCCTGCAAA ACCTGGCATC TCTGTATGGA 
GGCAGGCCCA GCTCTTCAAG AGGAGGGAAG CCAAGGAACA ATGAGGAAGA GGTTTATTTG 
GCAAGACTGA GGCAAATAAG ACTACAAAAT TTTAATGAGC GCCAACAGAT TAAAGCCAAA 
CTTCGTGGTG AGAATAAAGA AGCTGATGGT ACCAAAGGAC AAGAAGCAAC TGAAGAGACT 
GACATGAGGC TCAAAAAGAT GGAGTCACTT AAGGCGCAAA CAAATGCACG TGCTGCTGTA 
CTAAAAGAAC AGCTGGAGCG AAAAAGAAAG GAAGCTTATG AAAGAGAAAA GAAAGTATGG 
GAAGAACATT TGGTGGCGAG GGTAAAAAGC TCAGATGTTC CTCTGCCTTT GGAACTTCTT 
GAAACAGGTG GTTCTCCATC AAAGCAGCAG GTGAAGCCTG TCATTTCTGT GACTTCAGCT 
TTGAAAGAAG TGGGCCTGGA TGGAAGTTTA ACTGATACCC AGGAAGAAGA AATGGAAAAG 
AGTAACAGTG CTATTTCAAG TAAGCGAGAA ATCCTGCGTA GGCTAAATGA AAATCTTAAA 
GCTCAAGAGG ATGAAAAGGA AAAGCAGCAT CACTCAGGTT CTTGTGAGAC CGTTGGTCAC 
AAAGATGAGA GAGAGTATGA GACAGAAAAT GCCATTTCCT CTGATCGCAA GAAGTGGGAG 
ATGGGAGGTC AGCTTGTGAT TCCTCTCGAT GCAGTGACAC TGGATACATC CTTCTCTGCA 
ACCGAAAAAC ATACTGTGGG AGAGGTTATT AAATTAGATT CTAATGGCTC TCCAAGAAAA 
GTCTGGGGGA AAAACCCTAC AGATTCTGTG CTGAAGATAC TTGGAGAAGC TGAATTACAG 
CTACAGACAG AACTACTAGA AAACACATCT TTTAAAAGTG AGGTTTATGC TGAAGAGGAG 
AACTACAAAC CCTTACTTAC TGAAGAAGAG AATCTGCAGT GCATTTCAAA AGAAATAAAT 
CCATCAGCTA CTGTTGATTC TACTGAAACG AAAAGTCCAA AGTTTACTGA GGTGTCTCCA 
CAAATGTCAG AAGGAAATGT GGAAGAACCT GATGATTTGG AAACAGAAGT TCTACAAGAG 
CCAAGTAGCA CACACACAGA TGGGAGTTTG CCACCTGTTC TTAATGATGT GTGGACTAGA 
GAGAAGGAAG CAGCTAAGGA AACTGAGTTG GAAGATAAGG TTGCTGTGCA GCAGAGTGAA 
GTTTGTGAAG ATAGAATTCC AGGGAACGTG GACCAATCCT GTAAGGATCA GAGAGATCCT 
GCAGTAGACG ATTCTCCGCA GTCTGGCTGT GATGTAGAGA AGTCAGTACA GCCAGAATCG 
ATTTTCCAGA AAGTGGTTCA TTCTAAGGAC TTGAACTTAG TTCAGGCAGT TCATTGCTCA 
CCAGAAGAAC CAATTCCAAT TCGATCTCAC TCTGATTCTC CACCAAAAAC TAAGAGCAAG 
AATTCCTTAC TGATTGGACT TTCAACTGGT CTGTTTGATG CAAACAATCC AAAGATGCTG 
AGGACCTGCT CACTTCCAGA TCTTTCCAAG CTGTTCAGAA CCCTAATGGA CGTTCCCACT 
GTGGGGGACG TTCATCAAGA CAGTCTTGAA ATCGATGAGC TGGAAGATGA ACCAATTAAA 
GAAGGGCCTT CTGATTCCGA AGACACTGTA TTTGAAGAAA CTGACACAGA TTTACAAGAG 
CTTCAGGCCT CAATGGAGCA GCTGCTTAGG GAGCAACCAG GTGACGAATA CAGTGAGGAG 
GAAGAGTCTG TTTTAAAAAG CAGCGATGTG GAGCAGACAG CAAGAGGGAC AGATGCCCCA 
GACGAGGAGG ACAACCCCAG CAGCGAAAGC GCCCTGAACG AGGAATGGCA CTCAGATAAT 
AGTGACGCTG AGACCACTAG TGAATGTGAA TATGACAGTG TCTTTAACCA TTTAGAGGAA 
CTAAGACTTC ACTTGGAGCA AGAAATGGGC TTTGAAAAGT TCTTTGAGGT TTATGAGAAA 
GTAAAGGCTA TTCATGAGGA TGAAGATGAA AATATTGAAA TTTGTTCAAC AATAGTTGAG 
AATATTTTGG GCAATGAGCA CCAGCATCTC TATGCCAAGA TTCTGCATTT AGTCATGGCA 
GATGGAGCCT ATCAGGAAGA TAATGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168098.1
CDS	32..3784
Translation	MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERGVPPGVRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQVEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017312609.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312609.1

ORF Insert Sequence:

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 
GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 
TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 
CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 
GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 
ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 
AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 
CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 
CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 
CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 
TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 
TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 
TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 
GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 
GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 
ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGGC TTCCTTTTTT 
GGCATTGGAG GGGCTGTCTC TCCATCACCG TGTTCTCCTC GAGGCCAGTA TGAACATTAC 
CATGCCATTT TTGACCAAAT GCAGCGGCTA AGAGCAGAAG ATAATGAAGC AAGATGGAAG 
GGGGGAATCT ATGGTCGATG GCTCCCAGAA AGAGGAGTTC CACCTGGAGT TCGTCCAGGA 
TTTCCCAGTG AGGCTTCAGG TCACTACCAT TCCCCTGATG CTGGTGCTAT TAGGAAAACT 
CTGAAAAGAC TGAAGGCCGT GTCTAAGCAA GCCAGTACAA ACAGGCAAAA AGGACACTTA 
GCTGTAGAGA GAGCCAACCA AGTGGAAGAA TTCCTACAGC GTAAACGAGA AGCTATGCAG 
AATAAAGCCC GAGCCGAAGG ACACGTGGGA CTCCTGCAAA ACCTGGCATC TCTGTATGGA 
GGCAGGCCCA GCTCTTCAAG AGGAGGGAAG CCAAGGAACA ATGAGGAAGA GGTTTATTTG 
GCAAGACTGA GGCAAATAAG ACTACAAAAT TTTAATGAGC GCCAACAGAT TAAAGCCAAA 
CTTCGTGGTG AGAATAAAGA AGCTGATGGT ACCAAAGGAC AAGAAGCAAC TGAAGAGACT 
GACATGAGGC TCAAAAAGAT GGAGTCACTT AAGGCGCAAA CAAATGCACG TGCTGCTGTA 
CTAAAAGAAC AGCTGGAGCG AAAAAGAAAG GAAGCTTATG AAAGAGAAAA GAAAGTATGG 
GAAGAACATT TGGTGGCGAG GGTAAAAAGC TCAGATGTTC CTCTGCCTTT GGAACTTCTT 
GAAACAGGTG GTTCTCCATC AAAGCAGCAG GTGAAGCCTG TCATTTCTGT GACTTCAGCT 
TTGAAAGAAG TGGGCCTGGA TGGAAGTTTA ACTGATACCC AGGAAGAAGA AATGGAAAAG 
AGTAACAGTG CTATTTCAAG TAAGCGAGAA ATCCTGCGTA GGCTAAATGA AAATCTTAAA 
GCTCAAGAGG ATGAAAAGGA AAAGCAGCAT CACTCAGGTT CTTGTGAGAC CGTTGGTCAC 
AAAGATGAGA GAGAGTATGA GACAGAAAAT GCCATTTCCT CTGATCGCAA GAAGTGGGAG 
ATGGGAGGTC AGCTTGTGAT TCCTCTCGAT GCAGTGACAC TGGATACATC CTTCTCTGCA 
ACCGAAAAAC ATACTGTGGG AGAGGTTATT AAATTAGATT CTAATGGCTC TCCAAGAAAA 
GTCTGGGGGA AAAACCCTAC AGATTCTGTG CTGAAGATAC TTGGAGAAGC TGAATTACAG 
CTACAGACAG AACTACTAGA AAACACATCT TTTAAAAGTG AGGTTTATGC TGAAGAGGAG 
AACTACAAAC CCTTACTTAC TGAAGAAGAG AATCTGCAGT GCATTTCAAA AGAAATAAAT 
CCATCAGCTA CTGTTGATTC TACTGAAACG AAAAGTCCAA AGTTTACTGA GGTGTCTCCA 
CAAATGTCAG AAGGAAATGT GGAAGAACCT GATGATTTGG AAACAGAAGT TCTACAAGAG 
CCAAGTAGCA CACACACAGA TGGGAGTTTG CCACCTGTTC TTAATGATGT GTGGACTAGA 
GAGAAGGAAG CAGCTAAGGA AACTGAGTTG GAAGATAAGG TTGCTGTGCA GCAGAGTGAA 
GTTTGTGAAG ATAGAATTCC AGGGAACGTG GACCAATCCT GTAAGGATCA GAGAGATCCT 
GCAGTAGACG ATTCTCCGCA GTCTGGCTGT GATGTAGAGA AGTCAGTACA GCCAGAATCG 
ATTTTCCAGA AAGTGGTTCA TTCTAAGGAC TTGAACTTAG TTCAGGCAGT TCATTGCTCA 
CCAGAAGAAC CAATTCCAAT TCGATCTCAC TCTGATTCTC CACCAAAAAC TAAGAGCAAG 
AATTCCTTAC TGATTGGACT TTCAACTGGT CTGTTTGATG CAAACAATCC AAAGATGCTG 
AGGACCTGCT CACTTCCAGA TCTTTCCAAG CTGTTCAGAA CCCTAATGGA CGTTCCCACT 
GTGGGGGACG TTCATCAAGA CAGTCTTGAA ATCGATGAGC TGGAAGATGA ACCAATTAAA 
GAAGGGCCTT CTGATTCCGA AGACACTGTA TTTGAAGAAA CTGACACAGA TTTACAAGAG 
CTTCAGGCCT CAATGGAGCA GCTGCTTAGG GAGCAACCAG GTGACGAATA CAGTGAGGAG 
GAAGAGTCTG TTTTAAAAAG CAGCGATGTG GAGCAGACAG CAAGAGGGAC AGATGCCCCA 
GACGAGGAGG ACAACCCCAG CAGCGAAAGC GCCCTGAACG AGGAATGGCA CTCAGATAAT 
AGTGACGCTG AGACCACTAG TGAATGTGAA TATGACAGTG TCTTTAACCA TTTAGAGGAA 
CTAAGACTTC ACTTGGAGCA AGAAATGGGC TTTGAAAAGT TCTTTGAGGT TTATGAGAAA 
GTAAAGGCTA TTCATGAGGA TGAAGATGAA AATATTGAAA TTTGTTCAAC AATAGTTGAG 
AATATTTTGG GCAATGAGCA CCAGCATCTC TATGCCAAGA TTCTGCATTT AGTCATGGCA 
GATGGAGCCT ATCAGGAAGA TAATGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nek1
Entrez Gene ID	18004
Full Name	NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms	D8Ertd790e,kat
General protein information	Preferred Names NIMA (never in mitosis gene a)-related expressed kinase 1 Names serine/threonine-protein kinase Nek1 NIMA-related kinase 1 NY-REN-55 antigen Nek1 serine/threonine- and tyrosine-specific protein kinase kidney, anemia and testis never in mitosis A-related kinase 1 nimA-related protein kinase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.1\|8 30.91 cM

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nek1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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