Ppp1r15a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r15a
Entrez Gene ID	17872
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 15A
Synonyms	9630030H21,Gadd34,Myd116
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 15A Names protein phosphatase 1 regulatory subunit 15A growth arrest and DNA damage-inducible protein GADD34 growth arrest and DNA-damage-inducible 34 myeloid differentiation primary response gene 116 myeloid differentiation primary response protein MyD116
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_008654.2	NP_032680.1	protein phosphatase 1 regulatory subunit 15A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPP1R15A	XP_512805.2
	PPP1R15A	XP_001114376.1
Bos taurus (cattle)	PPP1R15A	NP_001039643.1
Homo sapiens (human)	PPP1R15A	NP_055145.3
Mus musculus (house mouse)	Ppp1r15a	NP_032680.1

Gene Ontology

The following Ppp1r15a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r15a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23128	NM_008654.2 Latest version!	Mus musculus protein phosphatase 1, regulatory subunit 15A (Ppp1r15a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r15a ( NM_008654.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032680.1 Mus musculus protein phosphatase 1, regulatory subunit 15A (Ppp1r15a), mRNA.

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CloneID	OMu23128
Clone ID Related Accession (Same CDS sequence)	NM_008654.2
Accession Version	NM_008654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 15A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151602.4, AK079339.1, BC138486.1 and AW493042.1. On Sep 17, 2009 this sequence version replaced NM_008654.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK136336.1, X51829.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 19131336 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCGA GCCCAAGACC CCAGCATGTC CTGCACTGGA GGGACGCCCA CAACTTCTAT 
CTCCTGTCCC CACTGATGGG CTTGCTCAGT CGGGCCTGGA GCCGCCTGAG GGGCCCAGAA 
GTCCCAGAGG CATGGCTGGC AAAAACAGTA ACAGGAGCAG ATCAGATAGA AGCTGCGGCT 
CTGCTGACAC CTACCCCTGT CTCTGGTAAC CTCCTCCCTC ATGGGGAGAC TGAAGAAAGT 
GGATCTCCTG AACAGAGTCA AGCAGCCCAG AGGCTCTGCC TTGTGGAAGC TGAAAGTTCC 
CCTCCTGAAA CTTGGGGACT TTCAAATGTT GATGAGTACA ATGCAAAGCC AGGACAAGAT 
GACCTTAGAG AGAAGGAAAT GGAACGCACA GCTGGCAAGG CCACACTACA GCCCGCTGGC 
CTGCAAGGGG CTGATAAGAG GCTTGGGGAG GTGGTGGCTA GAGAAGAGGG AGTGGCTGAG 
CCCGCTTATC CCACATCACA GCTGGAGGGT GGTCCAGCTG AGAATGAAGA GGATGGAGAA 
ACAGTGAAGA CTTACCAAGC TTCTGCTGCT TCCATAGCTC CGGGATACAA ACCCAGCACC 
CCTGTGCCTT TCTTGGGGGA GGCAGAACAT CAAGCCACGG AAGAAAAAGG AACAGAAAAC 
AAGGCTGACC CCTCCAACTC TCCTTCTTCA GGCTCCCACT CCAGAGCCTG GGAGTACTAC 
TCTAGAGAGA AGCCTAAGCA GGAGGGAGAA GCCAAGGTAG AGGCACACAG GGCAGGGCAG 
GGTCACCCTT GTCGGAATGC TGAGGCTGAG GAAGGAGGAC CTGAGACAAC TTTTGTCTGT 
ACTGGAAATG CCTTCCTGAA GGCCTGGGTG TATCGCCCAG GAGAGGACAC AGAGGAAGAA 
GACAACAGCG ATTCGGATTC AGCTGAGGAA GACACAGCTC AGACCGGTGC CACCCCCCAT 
ACAAGTGCCT TCCTGAAGGC CTGGGTGTAT CGCCCAGGAG AGGACACAGA GGAAGAAGAC 
AGCGATTCGG ATTCAGCTGA GGAAGACACA GCTCAGACCG GTGCCACCCC CCATACAAGT 
GCCTTCCTGA AGGCCTGGGT GTATCGCCCA GGAGAGGACA CAGAGGAAGA AAACAGCGAT 
TTGGATTCAG CTGAGGAAGA CACAGCTCAG ACCGGTGCCA CCCCCCATAC AAGTGCCTTC 
CTGAAGGCCT GGGTGTATCG CCCAGGAGAG GACACAGAGG AAGAAAACAG CGATTTGGAT 
TCAGCTGAGG AAGACACAGC TCAGACCGGT GCCACCCCAC ATACAAGTCC CTTCCTGAAG 
GCCTGGGTGT ATCGCCCAGG AGAGGACACA GAAGATGACA CAGAAGAGGA AGAGGACAGT 
GAGAATGTGG CCCCAGGTGA CTCAGAAACA GCTGACTCAA GCCAGAGTCC CTGCCTTCAG 
CCCCAGCGTT GTCTACCAGG AGAGAAGACC AAGGGACGTG GGGAAGAGCC CCCTCTCTTC 
CAGGTGGCCT TCTATTTACC CGGAGAGAAG CCAGAATCAC CTTGGGCTGC ACCTAAGCTG 
CCCCTTCGAC TGCAGAGGCG GCTCAGATTG TTCAAAGCCC CCACCCGGGA TCAGGACCCC 
GAGATTCCTC TAAAAGCTCG GAAGGTACAC TTCGCTGAGA AAGTCACAGT CCATTTCCTT 
GCTGTCTGGG CAGGACCAGC CCAAGCTGCC CGTCGAGGTC CCTGGGAGCA GTTTGCACGA 
GATCGAAGCC GCTTTGCTCG ACGCATTGCC CAGGCAGAGG AGAAGCTGGG TCCCTACCTT 
ACCCCTGATT CCAGGGCCAG AGCATGGGCA CGCCTTAGAA ACCCATCTCT TCCACAGTCC 
GAGCCTCGCT CTTCCTCTGA GGCCACTCCC TTGACCCAAG ATGTGACCAC ACCCTCTCCC 
CTTCCCAGTG AAACCCCTTC GCCCAGCCTG TACTTGGGAG GGAGGCGGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032680.1
CDS	284..2257
Translation	MAPSPRPQHVLHWRDAHNFYLLSPLMGLLSRAWSRLRGPEVPEAWLAKTVTGADQIEAAALLTPTPVSGNLLPHGETEESGSPEQSQAAQRLCLVEAESSPPETWGLSNVDEYNAKPGQDDLREKEMERTAGKATLQPAGLQGADKRLGEVVAREEGVAEPAYPTSQLEGGPAENEEDGETVKTYQASAASIAPGYKPSTPVPFLGEAEHQATEEKGTENKADPSNSPSSGSHSRAWEYYSREKPKQEGEAKVEAHRAGQGHPCRNAEAEEGGPETTFVCTGNAFLKAWVYRPGEDTEEEDNSDSDSAEEDTAQTGATPHTSAFLKAWVYRPGEDTEEEDSDSDSAEEDTAQTGATPHTSAFLKAWVYRPGEDTEEENSDLDSAEEDTAQTGATPHTSAFLKAWVYRPGEDTEEENSDLDSAEEDTAQTGATPHTSPFLKAWVYRPGEDTEDDTEEEEDSENVAPGDSETADSSQSPCLQPQRCLPGEKTKGRGEEPPLFQVAFYLPGEKPESPWAAPKLPLRLQRRLRLFKAPTRDQDPEIPLKARKVHFAEKVTVHFLAVWAGPAQAARRGPWEQFARDRSRFARRIAQAEEKLGPYLTPDSRARAWARLRNPSLPQSEPRSSSEATPLTQDVTTPSPLPSETPSPSLYLGGRRG

Target ORF information:

RefSeq Version	NM_008654.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory subunit 15A (Ppp1r15a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008654.2

ORF Insert Sequence:

ATGGCCCCGA GCCCAAGACC CCAGCATGTC CTGCACTGGA GGGACGCCCA CAACTTCTAT 
CTCCTGTCCC CACTGATGGG CTTGCTCAGT CGGGCCTGGA GCCGCCTGAG GGGCCCAGAA 
GTCCCAGAGG CATGGCTGGC AAAAACAGTA ACAGGAGCAG ATCAGATAGA AGCTGCGGCT 
CTGCTGACAC CTACCCCTGT CTCTGGTAAC CTCCTCCCTC ATGGGGAGAC TGAAGAAAGT 
GGATCTCCTG AACAGAGTCA AGCAGCCCAG AGGCTCTGCC TTGTGGAAGC TGAAAGTTCC 
CCTCCTGAAA CTTGGGGACT TTCAAATGTT GATGAGTACA ATGCAAAGCC AGGACAAGAT 
GACCTTAGAG AGAAGGAAAT GGAACGCACA GCTGGCAAGG CCACACTACA GCCCGCTGGC 
CTGCAAGGGG CTGATAAGAG GCTTGGGGAG GTGGTGGCTA GAGAAGAGGG AGTGGCTGAG 
CCCGCTTATC CCACATCACA GCTGGAGGGT GGTCCAGCTG AGAATGAAGA GGATGGAGAA 
ACAGTGAAGA CTTACCAAGC TTCTGCTGCT TCCATAGCTC CGGGATACAA ACCCAGCACC 
CCTGTGCCTT TCTTGGGGGA GGCAGAACAT CAAGCCACGG AAGAAAAAGG AACAGAAAAC 
AAGGCTGACC CCTCCAACTC TCCTTCTTCA GGCTCCCACT CCAGAGCCTG GGAGTACTAC 
TCTAGAGAGA AGCCTAAGCA GGAGGGAGAA GCCAAGGTAG AGGCACACAG GGCAGGGCAG 
GGTCACCCTT GTCGGAATGC TGAGGCTGAG GAAGGAGGAC CTGAGACAAC TTTTGTCTGT 
ACTGGAAATG CCTTCCTGAA GGCCTGGGTG TATCGCCCAG GAGAGGACAC AGAGGAAGAA 
GACAACAGCG ATTCGGATTC AGCTGAGGAA GACACAGCTC AGACCGGTGC CACCCCCCAT 
ACAAGTGCCT TCCTGAAGGC CTGGGTGTAT CGCCCAGGAG AGGACACAGA GGAAGAAGAC 
AGCGATTCGG ATTCAGCTGA GGAAGACACA GCTCAGACCG GTGCCACCCC CCATACAAGT 
GCCTTCCTGA AGGCCTGGGT GTATCGCCCA GGAGAGGACA CAGAGGAAGA AAACAGCGAT 
TTGGATTCAG CTGAGGAAGA CACAGCTCAG ACCGGTGCCA CCCCCCATAC AAGTGCCTTC 
CTGAAGGCCT GGGTGTATCG CCCAGGAGAG GACACAGAGG AAGAAAACAG CGATTTGGAT 
TCAGCTGAGG AAGACACAGC TCAGACCGGT GCCACCCCAC ATACAAGTCC CTTCCTGAAG 
GCCTGGGTGT ATCGCCCAGG AGAGGACACA GAAGATGACA CAGAAGAGGA AGAGGACAGT 
GAGAATGTGG CCCCAGGTGA CTCAGAAACA GCTGACTCAA GCCAGAGTCC CTGCCTTCAG 
CCCCAGCGTT GTCTACCAGG AGAGAAGACC AAGGGACGTG GGGAAGAGCC CCCTCTCTTC 
CAGGTGGCCT TCTATTTACC CGGAGAGAAG CCAGAATCAC CTTGGGCTGC ACCTAAGCTG 
CCCCTTCGAC TGCAGAGGCG GCTCAGATTG TTCAAAGCCC CCACCCGGGA TCAGGACCCC 
GAGATTCCTC TAAAAGCTCG GAAGGTACAC TTCGCTGAGA AAGTCACAGT CCATTTCCTT 
GCTGTCTGGG CAGGACCAGC CCAAGCTGCC CGTCGAGGTC CCTGGGAGCA GTTTGCACGA 
GATCGAAGCC GCTTTGCTCG ACGCATTGCC CAGGCAGAGG AGAAGCTGGG TCCCTACCTT 
ACCCCTGATT CCAGGGCCAG AGCATGGGCA CGCCTTAGAA ACCCATCTCT TCCACAGTCC 
GAGCCTCGCT CTTCCTCTGA GGCCACTCCC TTGACCCAAG ATGTGACCAC ACCCTCTCCC 
CTTCCCAGTG AAACCCCTTC GCCCAGCCTG TACTTGGGAG GGAGGCGGGG CTAA