Hsp90ab1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp90ab1
Entrez Gene ID	15516
Full Name	heat shock protein 90 alpha (cytosolic), class B member 1
Synonyms	90kDa,AL022974,C81438,Hsp84,Hsp84-1,Hsp90,Hspcb
General protein information	Preferred Names heat shock protein 90 alpha (cytosolic), class B member 1 Names heat shock protein HSP 90-beta HSP 84 TSTA heat shock 84 kDa heat shock 90kDa protein 1, beta heat shock protein 1, beta heat shock protein 90kDa alpha (cytosolic), class B member 1 heat shock protein, 84 kDa 1 retinal degeneration slow protein tumor-specific transplantation 84 kDa antigen
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B3\|17 22.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008302.3	NP_032328.2	heat shock protein HSP 90-beta

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsp83	NP_001261362.1
Arabidopsis thaliana (thale cress)	HSP90.1	NP_200076.1
Mus musculus (house mouse)	Hsp90ab1	NP_032328.2
Gallus gallus (chicken)	HSP90AB1	NP_996842.1
Saccharomyces cerevisiae (baker's yeast)	HSP82	NP_015084.1
Kluyveromyces lactis	KLLA0D12958g	XP_453640.1
Schizosaccharomyces pombe (fission yeast)	hsp90	NP_594365.1
Canis lupus familiaris (dog)	HSP90AB1	XP_532154.4
Bos taurus (cattle)	HSP90AB1	NP_001073105.1
Danio rerio (zebrafish)	hsp90ab1	NP_571385.2
Caenorhabditis elegans (roundworm)	daf-21	NP_506626.1
Neurospora crassa	NCU04142	XP_961298.1
Oryza sativa (rice)	Os08g0500700	NP_001062159.1
Homo sapiens (human)	HSP90AB1	NP_001258898.1
Pan troglodytes (chimpanzee)	HSP90AB1	XP_003311385.1
	HSP90AB1	XP_001099135.2
Rattus norvegicus (Norway rat)	Hsp90ab1	NP_001004082.3
Anopheles gambiae (African malaria mosquito)	HSP83_ANOGA	XP_308800.3
Eremothecium gossypii	AGOS_AFL148C	NP_985402.1
Magnaporthe oryzae (rice blast fungus)	MGG_06759	XP_003709473.1
Xenopus tropicalis (tropical clawed frog)	hsp90ab1	NP_001025655.1
Saccharomyces cerevisiae (baker's yeast)	HSC82	NP_013911.1

Gene Ontology
Bibliography

Related articles in PubMed

Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90.
Zhang G, Liu Z, Ding H, Zhou Y, Doan HA, Sin KWT, Zhu ZJ, Flores R, Wen Y, Gong X, Liu Q, Li YP
Nature communications8(1)589(2017 09)

Protein mediated regulation of the NHE1 isoform of the Na
Odunewu-Aderibigbe A, Fliegel L
Cellular signalling36145-153(2017 Aug)

PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.
Hall EA, Nahorski MS, Murray LM, Shaheen R, Perkins E, Dissanayake KN, Kristaryanto Y, Jones RA, Vogt J, Rivagorda M, Handley MT, Mali GR, Quidwai T, Soares DC, Keighren MA, McKie L, Mort RL, Gammoh N, Garcia-Munoz A, Davey T, Vermeren M, Walsh D, Budd P, Aligianis IA, Faqeih E, Quigley AJ, Jackson IJ, Kulathu Y, Jackson M, Ribchester RR, von Kriegsheim A, Alkuraya FS, Woods CG, Maher ER, Mill P
American journal of human genetics100(5)706-724(2017 May)

Hsp90 inhibition destabilizes Ezh2 protein in alloreactive T cells and reduces graft-versus-host disease in mice.
Huang Q, He S, Tian Y, Gu Y, Chen P, Li C, Huang J, Liu Y, Yu H, Jin M, Hu S, Tong Q, Ma A, Jin J, Hexner E, Fung H, Reshef R, Zhang Y, Zhang Y
Blood129(20)2737-2748(2017 05)

Multi-drug loaded micelles delivering chemotherapy and targeted therapies directed against HSP90 and the PI3K/AKT/mTOR pathway in prostate cancer.
Le B, Powers GL, Tam YT, Schumacher N, Malinowski RL, Steinke L, Kwon G, Marker PC
PloS one12(3)e0174658(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings identify the Ezh2-Hsp90 interaction as a previously unrecognized mechanism essential for T-cell responses and an effective target for controlling graft-versus-host disease.
Title: Hsp90 inhibition destabilizes Ezh2 protein in alloreactive T cells and reduces graft-versus-host disease in mice.

genes respond to HSP90 inhibition in a manner dependent on their genomic location with regard to strain-specific endogenous retroviruses-insertion sites.
Title: The evolutionary capacitor HSP90 buffers the regulatory effects of mammalian endogenous retroviruses.

Data show that docetaxel, rapamycin and tanespimycin multi-drug loaded micelles targeted against HSP90 and the PI3K/AKT/mTOR pathway in prostate cancer.
Title: Multi-drug loaded micelles delivering chemotherapy and targeted therapies directed against HSP90 and the PI3K/AKT/mTOR pathway in prostate cancer.

Hsp90 is highly expressed on the cell surface of melanoma cells, and synthetic agents that target Hsp90 are promising cancer therapeutic drugs
Title: The Ig V

a surface population of Hsp90 extracellularly binds TGFbetaRI and this complex behaves as an active participant in collagen production in TGFbeta-activated fibroblasts.
Title: Extracellular heat shock protein 90 binding to TGF? receptor I participates in TGF?-mediated collagen production in myocardial fibroblasts.

The following Hsp90ab1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp90ab1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16627	NM_008302.3 Latest version!	Mus musculus heat shock protein 90 alpha (cytosolic), class B member 1 (Hsp90ab1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp90ab1 ( NM_008302.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032328.2 Mus musculus heat shock protein 90 alpha (cytosolic), class B member 1 (Hsp90ab1), mRNA.

Price & Availability↑

CloneID	OMu16627
Clone ID Related Accession (Same CDS sequence)	NM_008302.3
Accession Version	NM_008302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Mus musculus(house mouse)
Product	heat shock protein HSP 90-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC163677.2. On Nov 17, 2006 this sequence version replaced NM_008302.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF465639.1, AK084097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGAGG AAGTGCACCA TGGAGAGGAG GAGGTGGAGA CCTTTGCCTT TCAGGCAGAA 
ATTGCCCAGC TCATGTCCCT CATCATCAAC ACTTTCTATT CAAACAAGGA GATTTTCCTC 
CGCGAGTTGA TCTCTAATGC TTCAGATGCC CTGGACAAGA TTCGATATGA GAGCCTGACG 
GACCCTTCTA AGTTGGACAG TGGGAAAGAG CTGAAAATTG ACATCATCCC CAACCCTCAG 
GAGCGCACGC TGACTTTGGT GGACACAGGC ATTGGCATGA CCAAGGCTGA CCTCATTAAT 
AACCTGGGAA CCATTGCTAA GTCTGGCACG AAGGCGTTCA TGGAGGCTCT CCAGGCTGGT 
GCAGACATCT CCATGATCGG GCAGTTTGGT GTCGGATTCT ACTCGGCCTA TCTAGTTGCA 
GAGAAAGTGG TTGTGATCAC GAAGCACAAT GATGATGAGC AGTATGCCTG GGAGTCGTCT 
GCGGGTGGCT CCTTCACCGT CCGGGCAGAC CATGGTGAGC CCATTGGCCG GGGTACCAAA 
GTGATCCTTC ACCTCAAAGA AGACCAGACA GAGTACTTGG AGGAGAGGAG GGTCAAGGAA 
GTGGTGAAGA AACATTCGCA GTTCATAGGC TATCCCATCA CCCTCTATTT GGAGAAGGAA 
CGGGAGAAGG AGATCAGTGA TGATGAGGCA GAGGAAGAGA AAGGTGAGAA AGAGGAGGAA 
GATAAGGAGG ATGAGGAGAA GCCTAAGATT GAAGATGTGG GATCCGATGA GGAAGATGAC 
AGCGGCAAAG ACAAGAAAAA GAAAACAAAG AAGATCAAAG AGAAGTACAT TGACCAGGAG 
GAGCTGAACA AGACAAAGCC TATCTGGACC AGAAACCCGG ATGACATCAC GCAGGAGGAG 
TATGGCGAAT TCTATAAGAG CCTCACCAAT GACTGGGAGG ACCACTTGGC AGTCAAGCAC 
TTCTCTGTAG AAGGTCAGTT GGAATTCAGG GCATTGCTCT TCATTCCCCG GCGGGCTCCC 
TTCGACCTTT TTGAGAACAA GAAGAAGAAG AACAACATCA AATTGTATGT CCGCCGTGTG 
TTCATCATGG ACAGCTGTGA CGAGCTGATA CCTGAGTACC TCAACTTTAT CCGCGGTGTG 
GTTGACTCCG AGGACCTGCC CCTGAACATC TCCCGGGAGA TGCTGCAGCA GAGCAAGATC 
CTGAAGGTCA TCCGCAAGAA CATCGTCAAG AAGTGCCTGG AGCTCTTCTC CGAGCTGGCT 
GAGGACAAGG AGAACTACAA GAAGTTCTAT GAGGCCTTCT CCAAAAATTT AAAGCTTGGA 
ATTCATGAAG ATTCCACTAA CCGACGCCGC CTCTCTGAGC TCCTTCGCTA TCACACCTCT 
CAGTCTGGAG ATGAGATGAC CTCCTTGTCA GAGTATGTGT CTCGCATGAA GGAGACCCAG 
AAGTCCATCT ACTATATCAC TGGTGAGAGC AAAGAGCAAG TGGCCAACTC TGCCTTTGTG 
GAGCGAGTGC GGAAGCGGGG CTTCGAGGTG GTGTATATGA CTGAGCCTAT TGACGAGTAC 
TGCGTGCAGC AGCTCAAGGA GTTTGATGGG AAGAGCCTGG TCTCAGTGAC TAAGGAGGGC 
CTGGAGCTAC CAGAGGACGA GGAAGAGAAG AAGAAAATGG AGGAGAGCAA GGCAAAGTTT 
GAGAATCTCT GCAAGCTCAT GAAGGAGATC TTGGACAAGA AGGTTGAAAA GGTGACAATC 
TCCAATAGGC TTGTGTCTTC ACCCTGCTGC ATTGTGACAA GCACCTATGG CTGGACAGCC 
AACATGGAAC GGATCATGAA GGCCCAGGCA CTGCGAGACA ACTCTACAAT GGGCTACATG 
ATGGCCAAAA AACACCTGGA GATCAACCCT GACCACCCCA TCGTGGAGAC CCTGCGGCAG 
AAGGCTGAGG CAGACAAAAA CGACAAAGCT GTCAAGGACC TGGTGGTGCT GCTGTTTGAA 
ACTGCTCTGC TCTCCTCTGG TTTCTCACTT GAGGATCCCC AAACCCACTC CAACCGCATC 
TACCGCATGA TTAAACTAGG CCTGGGCATC GATGAAGATG AGGTCACTGC AGAGGAGCCC 
AGTGCTGCTG TTCCTGATGA GATCCCCCCT CTGGAAGGCG ATGAGGATGC CTCGCGCATG 
GAAGAGGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032328.2
CDS	96..2270
Translation	MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKEDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVTAEEPSAAVPDEIPPLEGDEDASRMEEVD

Target ORF information:

RefSeq Version	NM_008302.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 90 alpha (cytosolic), class B member 1 (Hsp90ab1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008302.3

ORF Insert Sequence:

ATGCCTGAGG AAGTGCACCA TGGAGAGGAG GAGGTGGAGA CCTTTGCCTT TCAGGCAGAA 
ATTGCCCAGC TCATGTCCCT CATCATCAAC ACTTTCTATT CAAACAAGGA GATTTTCCTC 
CGCGAGTTGA TCTCTAATGC TTCAGATGCC CTGGACAAGA TTCGATATGA GAGCCTGACG 
GACCCTTCTA AGTTGGACAG TGGGAAAGAG CTGAAAATTG ACATCATCCC CAACCCTCAG 
GAGCGCACGC TGACTTTGGT GGACACAGGC ATTGGCATGA CCAAGGCTGA CCTCATTAAT 
AACCTGGGAA CCATTGCTAA GTCTGGCACG AAGGCGTTCA TGGAGGCTCT CCAGGCTGGT 
GCAGACATCT CCATGATCGG GCAGTTTGGT GTCGGATTCT ACTCGGCCTA TCTAGTTGCA 
GAGAAAGTGG TTGTGATCAC GAAGCACAAT GATGATGAGC AGTATGCCTG GGAGTCGTCT 
GCGGGTGGCT CCTTCACCGT CCGGGCAGAC CATGGTGAGC CCATTGGCCG GGGTACCAAA 
GTGATCCTTC ACCTCAAAGA AGACCAGACA GAGTACTTGG AGGAGAGGAG GGTCAAGGAA 
GTGGTGAAGA AACATTCGCA GTTCATAGGC TATCCCATCA CCCTCTATTT GGAGAAGGAA 
CGGGAGAAGG AGATCAGTGA TGATGAGGCA GAGGAAGAGA AAGGTGAGAA AGAGGAGGAA 
GATAAGGAGG ATGAGGAGAA GCCTAAGATT GAAGATGTGG GATCCGATGA GGAAGATGAC 
AGCGGCAAAG ACAAGAAAAA GAAAACAAAG AAGATCAAAG AGAAGTACAT TGACCAGGAG 
GAGCTGAACA AGACAAAGCC TATCTGGACC AGAAACCCGG ATGACATCAC GCAGGAGGAG 
TATGGCGAAT TCTATAAGAG CCTCACCAAT GACTGGGAGG ACCACTTGGC AGTCAAGCAC 
TTCTCTGTAG AAGGTCAGTT GGAATTCAGG GCATTGCTCT TCATTCCCCG GCGGGCTCCC 
TTCGACCTTT TTGAGAACAA GAAGAAGAAG AACAACATCA AATTGTATGT CCGCCGTGTG 
TTCATCATGG ACAGCTGTGA CGAGCTGATA CCTGAGTACC TCAACTTTAT CCGCGGTGTG 
GTTGACTCCG AGGACCTGCC CCTGAACATC TCCCGGGAGA TGCTGCAGCA GAGCAAGATC 
CTGAAGGTCA TCCGCAAGAA CATCGTCAAG AAGTGCCTGG AGCTCTTCTC CGAGCTGGCT 
GAGGACAAGG AGAACTACAA GAAGTTCTAT GAGGCCTTCT CCAAAAATTT AAAGCTTGGA 
ATTCATGAAG ATTCCACTAA CCGACGCCGC CTCTCTGAGC TCCTTCGCTA TCACACCTCT 
CAGTCTGGAG ATGAGATGAC CTCCTTGTCA GAGTATGTGT CTCGCATGAA GGAGACCCAG 
AAGTCCATCT ACTATATCAC TGGTGAGAGC AAAGAGCAAG TGGCCAACTC TGCCTTTGTG 
GAGCGAGTGC GGAAGCGGGG CTTCGAGGTG GTGTATATGA CTGAGCCTAT TGACGAGTAC 
TGCGTGCAGC AGCTCAAGGA GTTTGATGGG AAGAGCCTGG TCTCAGTGAC TAAGGAGGGC 
CTGGAGCTAC CAGAGGACGA GGAAGAGAAG AAGAAAATGG AGGAGAGCAA GGCAAAGTTT 
GAGAATCTCT GCAAGCTCAT GAAGGAGATC TTGGACAAGA AGGTTGAAAA GGTGACAATC 
TCCAATAGGC TTGTGTCTTC ACCCTGCTGC ATTGTGACAA GCACCTATGG CTGGACAGCC 
AACATGGAAC GGATCATGAA GGCCCAGGCA CTGCGAGACA ACTCTACAAT GGGCTACATG 
ATGGCCAAAA AACACCTGGA GATCAACCCT GACCACCCCA TCGTGGAGAC CCTGCGGCAG 
AAGGCTGAGG CAGACAAAAA CGACAAAGCT GTCAAGGACC TGGTGGTGCT GCTGTTTGAA 
ACTGCTCTGC TCTCCTCTGG TTTCTCACTT GAGGATCCCC AAACCCACTC CAACCGCATC 
TACCGCATGA TTAAACTAGG CCTGGGCATC GATGAAGATG AGGTCACTGC AGAGGAGCCC 
AGTGCTGCTG TTCCTGATGA GATCCCCCCT CTGGAAGGCG ATGAGGATGC CTCGCGCATG 
GAAGAGGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90.
Nature communications8(1)589(2017 09)
Zhang G,Liu Z,Ding H,Zhou Y,Doan HA,Sin KWT,Zhu ZJ,Flores R,Wen Y,Gong X,Liu Q,Li YP

Protein mediated regulation of the NHE1 isoform of the Na
Cellular signalling36145-153(2017 Aug)
Odunewu-Aderibigbe A,Fliegel L

PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.
American journal of human genetics100(5)706-724(2017 May)
Hall EA,Nahorski MS,Murray LM,Shaheen R,Perkins E,Dissanayake KN,Kristaryanto Y,Jones RA,Vogt J,Rivagorda M,Handley MT,Mali GR,Quidwai T,Soares DC,Keighren MA,McKie L,Mort RL,Gammoh N,Garcia-Munoz A,Davey T,Vermeren M,Walsh D,Budd P,Aligianis IA,Faqeih E,Quigley AJ,Jackson IJ,Kulathu Y,Jackson M,Ribchester RR,von Kriegsheim A,Alkuraya FS,Woods CG,Maher ER,Mill P

Hsp90 inhibition destabilizes Ezh2 protein in alloreactive T cells and reduces graft-versus-host disease in mice.
Blood129(20)2737-2748(2017 05)
Huang Q,He S,Tian Y,Gu Y,Chen P,Li C,Huang J,Liu Y,Yu H,Jin M,Hu S,Tong Q,Ma A,Jin J,Hexner E,Fung H,Reshef R,Zhang Y,Zhang Y

Multi-drug loaded micelles delivering chemotherapy and targeted therapies directed against HSP90 and the PI3K/AKT/mTOR pathway in prostate cancer.
PloS one12(3)e0174658(2017)
Le B,Powers GL,Tam YT,Schumacher N,Malinowski RL,Steinke L,Kwon G,Marker PC