H1f0 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H1f0
Entrez Gene ID	14958
Full Name	H1 histone family, member 0
Synonyms	D130017D06Rik,H1(0),H1fv
General protein information	Preferred Names H1 histone family, member 0 Names histone H1.0 H1-0 histone histone H1' histone H1(0) myD196
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 E1\|15 37.7 cM
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008197.3	NP_032223.2	histone H1.0

Pathways from BioSystems

Homologies

	H1F0	XP_001088913.2
Homo sapiens (human)	H1F0	NP_005309.1
Mus musculus (house mouse)	H1f0	NP_032223.2
Pan troglodytes (chimpanzee)	LOC743278	XP_001147037.2

Gene Ontology
Bibliography

Related articles in PubMed

Temporally and Spatially Regulated Expression of the Linker Histone H1fx During Mouse Development.
Ichihara-Tanaka K, Kadomatsu K, Kishida S
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society65(9)513-530(2017 09)

Role of H1 linker histones in mammalian development and stem cell differentiation.
Pan C, Fan Y
Biochimica et biophysica acta1859(3)496-509(2016 Mar)

Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.
Mayor R, Izquierdo-Bouldstridge A, Mill?n-Ari?o L, Bustillos A, Sampaio C, Luque N, Jordan A
The Journal of biological chemistry290(12)7474-91(2015 Mar)

Reduction of Hox gene expression by histone H1 depletion.
Zhang Y, Liu Z, Medrzycki M, Cao K, Fan Y
PloS one7(6)e38829(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both P-TEFb activity and H1 phosphorylation are necessary for the full differentiation of C2C12 myoblasts into myotubes.
Title: Phosphorylation of histone H1 by P-TEFb is a necessary step in skeletal muscle differentiation.

Marked reduction in total H1 levels causes significant reduction in both expression and the level of active histone mark H3K4me3 at many Hox genes.
Title: Reduction of Hox gene expression by histone H1 depletion.

Histone H1 poly[ADP]-ribosylation of proteins over a specific time course is required for the changes in synaptic plasticity related to memory stabilization in mice.
Title: Histone H1 poly[ADP]-ribosylation regulates the chromatin alterations required for learning consolidation.

Analysis of DNA-induced secondary structure of the carboxyl-terminal domain of histone H1 subtypes H1(0)(C-H1(0)) and H1t (C-H1t).
Title: DNA-induced secondary structure of the carboxyl-terminal domain of histone H1.

H1 dephosphorylation and H2A.X hyperphosphorylation are necessary steps on the apoptotic pathway.
Title: Hyperphosphorylation of histone H2A.X and dephosphorylation of histone H1 subtypes in the course of apoptosis.

The following H1f0 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H1f0 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20165	NM_008197.3 Latest version!	Mus musculus H1.0 linker histone (H1f0), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H1f0 ( NM_008197.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032223.2 Mus musculus H1.0 linker histone (H1f0), mRNA.

Price & Availability↑

CloneID	OMu20165
Clone ID Related Accession (Same CDS sequence)	NM_008197.3
Accession Version	NM_008197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	histone H1.0
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB661016.1, AK051213.1 and AI849476.1. On Jan 27, 2006 this sequence version replaced NM_008197.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## replication-independent histone :: PMID: 25645921 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCGAGA ACTCCACCTC CGCCCCGGCG GCGAAGCCCA AACGGGCCAA GGCTTCCAAG 
AAGTCCACGG ACCACCCCAA GTATTCAGAC ATGATCGTGG CTGCTATCCA GGCAGAGAAG 
AACCGTGCCG GCTCCTCGCG CCAGTCCATC CAAAAGTATA TCAAGAGCCA CTACAAGGTG 
GGTGAGAACG CCGACTCCCA GATCAAGTTG TCCATCAAGC GCCTAGTGAC CACCGGTGTT 
CTCAAGCAAA CCAAAGGGGT GGGCGCCTCG GGGTCCTTCA GGCTGGCCAA GGGCGATGAG 
CCCAAAAGGT CGGTGGCTTT CAAGAAGACC AAGAAGGAAG TCAAGAAAGT GGCCACTCCA 
AAGAAGGCAG CCAAGCCCAA GAAGGCTGCC TCCAAAGCCC CAAGCAAGAA ACCCAAAGCC 
ACCCCTGTCA AGAAGGCCAA GAAGAAGCCG GCTGCCACGC CCAAGAAAGC CAAAAAGCCC 
AAGGTTGTCA AAGTCAAACC AGTCAAGGCC TCCAAACCCA AGAAGGCCAA AACCGTGAAG 
CCCAAAGCCA AGTCGAGTGC CAAGAGGGCC AGCAAGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032223.2
CDS	511..1095
Translation	MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKGDEPKRSVAFKKTKKEVKKVATPKKAAKPKKAASKAPSKKPKATPVKKAKKKPAATPKKAKKPKVVKVKPVKASKPKKAKTVKPKAKSSAKRASKKK

Target ORF information:

RefSeq Version	NM_008197.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus H1.0 linker histone (H1f0), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008197.3

ORF Insert Sequence:

ATGACCGAGA ACTCCACCTC CGCCCCGGCG GCGAAGCCCA AACGGGCCAA GGCTTCCAAG 
AAGTCCACGG ACCACCCCAA GTATTCAGAC ATGATCGTGG CTGCTATCCA GGCAGAGAAG 
AACCGTGCCG GCTCCTCGCG CCAGTCCATC CAAAAGTATA TCAAGAGCCA CTACAAGGTG 
GGTGAGAACG CCGACTCCCA GATCAAGTTG TCCATCAAGC GCCTAGTGAC CACCGGTGTT 
CTCAAGCAAA CCAAAGGGGT GGGCGCCTCG GGGTCCTTCA GGCTGGCCAA GGGCGATGAG 
CCCAAAAGGT CGGTGGCTTT CAAGAAGACC AAGAAGGAAG TCAAGAAAGT GGCCACTCCA 
AAGAAGGCAG CCAAGCCCAA GAAGGCTGCC TCCAAAGCCC CAAGCAAGAA ACCCAAAGCC 
ACCCCTGTCA AGAAGGCCAA GAAGAAGCCG GCTGCCACGC CCAAGAAAGC CAAAAAGCCC 
AAGGTTGTCA AAGTCAAACC AGTCAAGGCC TCCAAACCCA AGAAGGCCAA AACCGTGAAG 
CCCAAAGCCA AGTCGAGTGC CAAGAGGGCC AGCAAGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporally and Spatially Regulated Expression of the Linker Histone H1fx During Mouse Development.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society65(9)513-530(2017 09)
Ichihara-Tanaka K,Kadomatsu K,Kishida S

Role of H1 linker histones in mammalian development and stem cell differentiation.
Biochimica et biophysica acta1859(3)496-509(2016 Mar)
Pan C,Fan Y

Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.
The Journal of biological chemistry290(12)7474-91(2015 Mar)
Mayor R,Izquierdo-Bouldstridge A,Mill?n-Ari?o L,Bustillos A,Sampaio C,Luque N,Jordan A

Reduction of Hox gene expression by histone H1 depletion.
PloS one7(6)e38829(2012)
Zhang Y,Liu Z,Medrzycki M,Cao K,Fan Y