Rhbdf1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006514499.3	XP_006514562.1	inactive rhomboid protein 1 isoform X4
XM_006514494.4	XP_006514557.1	inactive rhomboid protein 1 isoform X2
XM_006514499.4	XP_006514562.1	inactive rhomboid protein 1 isoform X3
XM_006514503.2	XP_006514566.1	inactive rhomboid protein 1 isoform X6
XM_006514494.3	XP_006514557.1	inactive rhomboid protein 1 isoform X3
XM_011243658.2	XP_011241960.1	inactive rhomboid protein 1 isoform X1
XM_006514496.3	XP_006514559.1	inactive rhomboid protein 1 isoform X2
XM_011243657.2	XP_011241959.1	inactive rhomboid protein 1 isoform X1
NM_001291818.1	NP_001278747.1	inactive rhomboid protein 1 isoform 2
XM_006514501.3	XP_006514564.1	inactive rhomboid protein 1 isoform X5
XM_030245523.1	XP_030101383.1	inactive rhomboid protein 1 isoform X3
XM_030245524.1	XP_030101384.1	inactive rhomboid protein 1 isoform X4
XM_006514501.4	XP_006514564.1	inactive rhomboid protein 1 isoform X5
XM_030245526.1	XP_030101386.1	inactive rhomboid protein 1 isoform X7
NM_010117.2	NP_034247.2	inactive rhomboid protein 1 isoform 1
XM_006514495.3	XP_006514558.1	inactive rhomboid protein 1 isoform X3
XM_006514495.4	XP_006514558.1	inactive rhomboid protein 1 isoform X2
XM_030245525.1	XP_030101385.1	inactive rhomboid protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19064	NM_001291818.1 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu45072	XM_006514503.2 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu45066	XM_006514496.3 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu67606	XM_011243657.2 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu67607	XM_011243658.2 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu19067	NM_010117.2 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
OMu19067	XM_030245525.1 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu19067	XM_030245524.1 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu45066	XM_006514494.4 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu45066	XM_006514495.4 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu45069	XM_030245523.1 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu45069	XM_006514499.4 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu45071	XM_006514501.4 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu85618	XM_030245526.1 Latest version!	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu45069	XM_006514499.3	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu45066	XM_006514494.3	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu45071	XM_006514501.3	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu45066	XM_006514495.3	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

Rhbdf1 ( NM_001291818.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278747.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19064
Clone ID Related Accession (Same CDS sequence)	NM_001291818.1
Accession Version	NM_001291818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY766909.1, AK144437.1, AL929446.5 and AW491627.1. On Apr 29, 2014 this sequence version replaced XM_006514502.1. Transcript Variant: This variant (2) use an alternate splice junction at the 5' end of an exon and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144437.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTTCC GGGCCGCTGC AGCATTGGTG AAGGGTCGGT CTATTAGGGA TGGCACTTTA 
CGAAGGGGAC AGCGCCGAAG CTTTACCCCT GCCAGCTTTC TTGAGGAAGA CATGGTGGAC 
TTCCCTGATG AGTTGGATAC ATCTTTCTTT GCTCGGGAAG GTGTCCTCCA CGAGGAGATG 
TCCACGTACC CAGATGAGGT GTTTGAGTCC CCATCAGAGG CAGCGCTCAA GGACTGGGAG 
AAAGCCCCGG ATCAGGCCGA CCTCACGGGT GGGGCCCTGG ACCGCAGTGA GCTTGAGAGA 
AGCCACTTGA TGTTGCCCCT GGAACGAGGC TGGAGAAAGC AGAAGGAAGG CGGCCCTCTA 
GCCCCGCAGC CCAAGGTGCG GCTTCGCCAG GAGGTGGTCA GTGCGGCGGG ACCCAGGAGG 
GGTCAGCGTA TCGCTGTGCC AGTGCGCAAA CTCTTCGCTC GGGAAAAGCG GCCTTATGGT 
CTGGGGATGG TGGGACGGCT CACCAACCGC ACGTACCGGA AACGCATCGA CAGCTATGTC 
AAGCGGCAGA TCGAGGACAT GGATGATCAC AGGCCCTTCT TCACTTACTG GCTCACCTTT 
GTGCACTCAC TGGTCACCAT TCTGGCTGTG TGCATCTATG GCATAGCGCC CGTGGGCTTC 
TCGCAGCATG AGACGGTGGA CTCGGTACTT CGGAAGCGGG GTGTCTATGA GAATGTCAAA 
TACGTCCAGC AGGAGAACTT CTGGATTGGC CCCAGCTCGG AGGCCCTCAT TCACTTGGGT 
GCCAAGTTCT CACCCTGCAT GCGTCAGGAC CCACAGGTGC ACAGCTTCAT CCTGGCTGCC 
CGGGAGCGTG AAAAGCACTC GGCTTGCTGT GTACGCAATG ATCGGTCTGG GTGTGTGCAG 
ACTTCCAAGG AGGAGTGCTC GTCCACGTTG GCGGTGTGGG TGAAGTGGCC CGTTCATCCT 
AGTGCCCCAG ACCTTGCAGG CAACAAGAGA CAGTTTGGTT CTGTCTGCCA CCAGGACCCC 
AGGGTATGTG ACGAGCCTTC CTCTGAGGAT CCCCACGAGT GGCCAGAAGA TATCACCAAG 
TGGCCGATCT GCACCAAAAG CAGCGCGGGG AACCACACTA ACCACCCTCA CATGGATTGT 
GTCATAACAG GCCGGCCTTG CTGCATCGGC ACCAAGGGCA GGTGTGAGAT CACCTCCCGG 
GAGTACTGTG ACTTCATGAG GGGCTACTTC CATGAGGAGG CCACACTCTG CTCTCAGGTG 
CACTGCATGG ACGATGTCTG TGGCCTCCTG CCTTTCCTCA ATCCTGAGGT GCCTGACCAG 
TTCTACCGCC TGTGGCTGTC CCTCTTCCTG CACGCTGGGA TCCTGCACTG CTTGGTGTCC 
GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG GCACCGCATA 
GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT CTTCCTGCCA 
TACCGGGCAG AGGTAGGTCC GGCCGGCTCC CAGTTTGGCA TCCTGGCCTG CCTCTTTGTG 
GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC GTGCCTTCTT CAAGCTCCTG 
GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT GGATCGACAA CTTCGCCCAC 
ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT TCCTGCCTTA CATCAGCTTT 
GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA TCTTCCAGGT CGTCTTCCTG 
GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC CGGTGCGCTG TGAATGGTGC 
GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG AAAAGTACGA GCTGGACGCT 
CAGCTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278747.1
CDS	670..2601
Translation	MSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDNFAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	NM_001291818.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291818.1

ORF Insert Sequence:

ATGAGCTTCC GGGCCGCTGC AGCATTGGTG AAGGGTCGGT CTATTAGGGA TGGCACTTTA 
CGAAGGGGAC AGCGCCGAAG CTTTACCCCT GCCAGCTTTC TTGAGGAAGA CATGGTGGAC 
TTCCCTGATG AGTTGGATAC ATCTTTCTTT GCTCGGGAAG GTGTCCTCCA CGAGGAGATG 
TCCACGTACC CAGATGAGGT GTTTGAGTCC CCATCAGAGG CAGCGCTCAA GGACTGGGAG 
AAAGCCCCGG ATCAGGCCGA CCTCACGGGT GGGGCCCTGG ACCGCAGTGA GCTTGAGAGA 
AGCCACTTGA TGTTGCCCCT GGAACGAGGC TGGAGAAAGC AGAAGGAAGG CGGCCCTCTA 
GCCCCGCAGC CCAAGGTGCG GCTTCGCCAG GAGGTGGTCA GTGCGGCGGG ACCCAGGAGG 
GGTCAGCGTA TCGCTGTGCC AGTGCGCAAA CTCTTCGCTC GGGAAAAGCG GCCTTATGGT 
CTGGGGATGG TGGGACGGCT CACCAACCGC ACGTACCGGA AACGCATCGA CAGCTATGTC 
AAGCGGCAGA TCGAGGACAT GGATGATCAC AGGCCCTTCT TCACTTACTG GCTCACCTTT 
GTGCACTCAC TGGTCACCAT TCTGGCTGTG TGCATCTATG GCATAGCGCC CGTGGGCTTC 
TCGCAGCATG AGACGGTGGA CTCGGTACTT CGGAAGCGGG GTGTCTATGA GAATGTCAAA 
TACGTCCAGC AGGAGAACTT CTGGATTGGC CCCAGCTCGG AGGCCCTCAT TCACTTGGGT 
GCCAAGTTCT CACCCTGCAT GCGTCAGGAC CCACAGGTGC ACAGCTTCAT CCTGGCTGCC 
CGGGAGCGTG AAAAGCACTC GGCTTGCTGT GTACGCAATG ATCGGTCTGG GTGTGTGCAG 
ACTTCCAAGG AGGAGTGCTC GTCCACGTTG GCGGTGTGGG TGAAGTGGCC CGTTCATCCT 
AGTGCCCCAG ACCTTGCAGG CAACAAGAGA CAGTTTGGTT CTGTCTGCCA CCAGGACCCC 
AGGGTATGTG ACGAGCCTTC CTCTGAGGAT CCCCACGAGT GGCCAGAAGA TATCACCAAG 
TGGCCGATCT GCACCAAAAG CAGCGCGGGG AACCACACTA ACCACCCTCA CATGGATTGT 
GTCATAACAG GCCGGCCTTG CTGCATCGGC ACCAAGGGCA GGTGTGAGAT CACCTCCCGG 
GAGTACTGTG ACTTCATGAG GGGCTACTTC CATGAGGAGG CCACACTCTG CTCTCAGGTG 
CACTGCATGG ACGATGTCTG TGGCCTCCTG CCTTTCCTCA ATCCTGAGGT GCCTGACCAG 
TTCTACCGCC TGTGGCTGTC CCTCTTCCTG CACGCTGGGA TCCTGCACTG CTTGGTGTCC 
GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG GCACCGCATA 
GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT CTTCCTGCCA 
TACCGGGCAG AGGTAGGTCC GGCCGGCTCC CAGTTTGGCA TCCTGGCCTG CCTCTTTGTG 
GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC GTGCCTTCTT CAAGCTCCTG 
GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT GGATCGACAA CTTCGCCCAC 
ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT TCCTGCCTTA CATCAGCTTT 
GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA TCTTCCAGGT CGTCTTCCTG 
GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC CGGTGCGCTG TGAATGGTGC 
GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG AAAAGTACGA GCTGGACGCT 
CAGCTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514501.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514564.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu45071
Clone ID Related Accession (Same CDS sequence)	XM_006514501.3 , XM_006514501.4
Accession Version	XM_006514501.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC GGGCCGCTGC AGCATTGGTG AAGGGTCGGT CTATTAGGGA TGGCACTTTA 
CGAAGGGGAC AGCGCCGAAG CTTTACCCCT GCCAGCTTTC TTGAGGAAGA CATGGTGGAC 
TTCCCTGATG AGTTGGATAC ATCTTTCTTT GCTCGGGAAG GTGTCCTCCA CGAGGAGATG 
TCCACGTACC CAGATGAGGT GTTTGAGTCC CCATCAGAGG CAGCGCTCAA GGACTGGGAG 
AAAGCCCCGG ATCAGGCCGA CCTCACGGGT GGGGCCCTGG ACCGCAGTGA GCTTGAGAGA 
AGCCACTTGA TGTTGCCCCT GGAACGAGGC TGGAGAAAGC AGAAGGAAGG CGGCCCTCTA 
GCCCCGCAGC CCAAGGTGCG GCTTCGCCAG GAGGTGGTCA GTGCGGCGGG ACCCAGGAGG 
GGTCAGCGTA TCGCTGTGCC AGTGCGCAAA CTCTTCGCTC GGGAAAAGCG GCCTTATGGT 
CTGGGGATGG TGGGACGGCT CACCAACCGC ACGTACCGGA AACGCATCGA CAGCTATGTC 
AAGCGGCAGA TCGAGGACAT GGATGATCAC AGGCCCTTCT TCACTTACTG GCTCACCTTT 
GTGCACTCAC TGGTCACCAT TCTGGCTGTG TGCATCTATG GCATAGCGCC CGTGGGCTTC 
TCGCAGCATG AGACGGTGGA CTCGGTACTT CGGAAGCGGG GTGTCTATGA GAATGTCAAA 
TACGTCCAGC AGGAGAACTT CTGGATTGGC CCCAGCTCGG AGGCCCTCAT TCACTTGGGT 
GCCAAGTTCT CACCCTGCAT GCGTCAGGAC CCACAGGTGC ACAGCTTCAT CCTGGCTGCC 
CGGGAGCGTG AAAAGCACTC GGCTTGCTGT GTACGCAATG ATCGGTCTGG GTGTGTGCAG 
ACTTCCAAGG AGGAGTGCTC GTCCACGTTG GCGGTGTGGG TGAAGTGGCC CGTTCATCCT 
AGTGCCCCAG ACCTTGCAGG CAACAAGAGA CAGTTTGGTT CTGTCTGCCA CCAGGACCCC 
AGGGTATGTG ACGAGCCTTC CTCTGAGGAT CCCCACGAGT GGCCAGAAGA TATCACCAAG 
TGGCCGATCT GCACCAAAAG CAGCGCGGGG AACCACACTA ACCACCCTCA CATGGATTGT 
GTCATAACAG GCCGGCCTTG CTGCATCGGC ACCAAGGGCA GGTGTGAGAT CACCTCCCGG 
GAGTACTGTG ACTTCATGAG GGGCTACTTC CATGAGGAGG CCACACTCTG CTCTCAGGTG 
CACTGCATGG ACGATGTCTG TGGCCTCCTG CCTTTCCTCA ATCCTGAGGT GCCTGACCAG 
TTCTACCGCC TGTGGCTGTC CCTCTTCCTG CACGCTGGGA TCCTGCACTG CTTGGTGTCC 
GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG GCACCGCATA 
GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT CTTCCTGCCA 
TACCGGGCAG AGGTCCGGCC GGCTCCCAGT TTGGCATCCT GGCCTGCCTC TTTGTGGAGT 
TGTTCCAGAG CTGGCAGATC CTGGCCCGAC CCTGGCGTGC CTTCTTCAAG CTCCTGGCCG 
TGGTGCTCTT CCTCTTTGCC TTTGGGCTGC TGCCCTGGAT CGACAACTTC GCCCACATCT 
CGGGCTTTGT CAGCGGCCTC TTCCTTTCCT TTGCCTTCCT GCCTTACATC AGCTTTGGCA 
AGTTCGACCT GTACCGCAAG CGCTGCCAGA TCATCATCTT CCAGGTCGTC TTCCTGGGCC 
TGCTGGCCGG CCTGGTGGTC CTCTTCTACT TCTACCCGGT GCGCTGTGAA TGGTGCGAGT 
TCCTCACCTG CATCCCTTTC ACGGACAAGT TCTGTGAAAA GTACGAGCTG GACGCTCAGC 
TCCACTGAAC CAGCCGTGGA CTCCCCAGGC CAGCCCGGCC TCCCAGGGCT GAACAGATGG 
GTGCTTCCTG GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514564.1
CDS	709..2706
Translation	MSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514501.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514501.3

ORF Insert Sequence:

ATGAGCTTCC GGGCCGCTGC AGCATTGGTG AAGGGTCGGT CTATTAGGGA TGGCACTTTA 
CGAAGGGGAC AGCGCCGAAG CTTTACCCCT GCCAGCTTTC TTGAGGAAGA CATGGTGGAC 
TTCCCTGATG AGTTGGATAC ATCTTTCTTT GCTCGGGAAG GTGTCCTCCA CGAGGAGATG 
TCCACGTACC CAGATGAGGT GTTTGAGTCC CCATCAGAGG CAGCGCTCAA GGACTGGGAG 
AAAGCCCCGG ATCAGGCCGA CCTCACGGGT GGGGCCCTGG ACCGCAGTGA GCTTGAGAGA 
AGCCACTTGA TGTTGCCCCT GGAACGAGGC TGGAGAAAGC AGAAGGAAGG CGGCCCTCTA 
GCCCCGCAGC CCAAGGTGCG GCTTCGCCAG GAGGTGGTCA GTGCGGCGGG ACCCAGGAGG 
GGTCAGCGTA TCGCTGTGCC AGTGCGCAAA CTCTTCGCTC GGGAAAAGCG GCCTTATGGT 
CTGGGGATGG TGGGACGGCT CACCAACCGC ACGTACCGGA AACGCATCGA CAGCTATGTC 
AAGCGGCAGA TCGAGGACAT GGATGATCAC AGGCCCTTCT TCACTTACTG GCTCACCTTT 
GTGCACTCAC TGGTCACCAT TCTGGCTGTG TGCATCTATG GCATAGCGCC CGTGGGCTTC 
TCGCAGCATG AGACGGTGGA CTCGGTACTT CGGAAGCGGG GTGTCTATGA GAATGTCAAA 
TACGTCCAGC AGGAGAACTT CTGGATTGGC CCCAGCTCGG AGGCCCTCAT TCACTTGGGT 
GCCAAGTTCT CACCCTGCAT GCGTCAGGAC CCACAGGTGC ACAGCTTCAT CCTGGCTGCC 
CGGGAGCGTG AAAAGCACTC GGCTTGCTGT GTACGCAATG ATCGGTCTGG GTGTGTGCAG 
ACTTCCAAGG AGGAGTGCTC GTCCACGTTG GCGGTGTGGG TGAAGTGGCC CGTTCATCCT 
AGTGCCCCAG ACCTTGCAGG CAACAAGAGA CAGTTTGGTT CTGTCTGCCA CCAGGACCCC 
AGGGTATGTG ACGAGCCTTC CTCTGAGGAT CCCCACGAGT GGCCAGAAGA TATCACCAAG 
TGGCCGATCT GCACCAAAAG CAGCGCGGGG AACCACACTA ACCACCCTCA CATGGATTGT 
GTCATAACAG GCCGGCCTTG CTGCATCGGC ACCAAGGGCA GGTGTGAGAT CACCTCCCGG 
GAGTACTGTG ACTTCATGAG GGGCTACTTC CATGAGGAGG CCACACTCTG CTCTCAGGTG 
CACTGCATGG ACGATGTCTG TGGCCTCCTG CCTTTCCTCA ATCCTGAGGT GCCTGACCAG 
TTCTACCGCC TGTGGCTGTC CCTCTTCCTG CACGCTGGGA TCCTGCACTG CTTGGTGTCC 
GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG GCACCGCATA 
GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT CTTCCTGCCA 
TACCGGGCAG AGGTCCGGCC GGCTCCCAGT TTGGCATCCT GGCCTGCCTC TTTGTGGAGT 
TGTTCCAGAG CTGGCAGATC CTGGCCCGAC CCTGGCGTGC CTTCTTCAAG CTCCTGGCCG 
TGGTGCTCTT CCTCTTTGCC TTTGGGCTGC TGCCCTGGAT CGACAACTTC GCCCACATCT 
CGGGCTTTGT CAGCGGCCTC TTCCTTTCCT TTGCCTTCCT GCCTTACATC AGCTTTGGCA 
AGTTCGACCT GTACCGCAAG CGCTGCCAGA TCATCATCTT CCAGGTCGTC TTCCTGGGCC 
TGCTGGCCGG CCTGGTGGTC CTCTTCTACT TCTACCCGGT GCGCTGTGAA TGGTGCGAGT 
TCCTCACCTG CATCCCTTTC ACGGACAAGT TCTGTGAAAA GTACGAGCTG GACGCTCAGC 
TCCACTGAAC CAGCCGTGGA CTCCCCAGGC CAGCCCGGCC TCCCAGGGCT GAACAGATGG 
GTGCTTCCTG GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514503.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514566.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu45072
Clone ID Related Accession (Same CDS sequence)	XM_006514503.2
Accession Version	XM_006514503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACGT ACCCAGATGA GGTGTTTGAG TCCCCATCAG AGGCAGCGCT CAAGGACTGG 
GAGAAAGCCC CGGATCAGGC CGACCTCACG GGTGGGGCCC TGGACCGCAG TGAGCTTGAG 
AGAAGCCACT TGATGTTGCC CCTGGAACGA GGCTGGAGAA AGCAGAAGGA AGGCGGCCCT 
CTAGCCCCGC AGCCCAAGGT GCGGCTTCGC CAGGAGGTGG TCAGTGCGGC GGGACCCAGG 
AGGGGTCAGC GTATCGCTGT GCCAGTGCGC AAACTCTTCG CTCGGGAAAA GCGGCCTTAT 
GGTCTGGGGA TGGTGGGACG GCTCACCAAC CGCACGTACC GGAAACGCAT CGACAGCTAT 
GTCAAGCGGC AGATCGAGGA CATGGATGAT CACAGGCCCT TCTTCACTTA CTGGCTCACC 
TTTGTGCACT CACTGGTCAC CATTCTGGCT GTGTGCATCT ATGGCATAGC GCCCGTGGGC 
TTCTCGCAGC ATGAGACGGT GGACTCGGTA CTTCGGAAGC GGGGTGTCTA TGAGAATGTC 
AAATACGTCC AGCAGGAGAA CTTCTGGATT GGCCCCAGCT CGGAGGCCCT CATTCACTTG 
GGTGCCAAGT TCTCACCCTG CATGCGTCAG GACCCACAGG TGCACAGCTT CATCCTGGCT 
GCCCGGGAGC GTGAAAAGCA CTCGGCTTGC TGTGTACGCA ATGATCGGTC TGGGTGTGTG 
CAGACTTCCA AGGAGGAGTG CTCGTCCACG TTGGCGGTGT GGGTGAAGTG GCCCGTTCAT 
CCTAGTGCCC CAGACCTTGC AGGCAACAAG AGACAGTTTG GTTCTGTCTG CCACCAGGAC 
CCCAGGGTAT GTGACGAGCC TTCCTCTGAG GATCCCCACG AGTGGCCAGA AGATATCACC 
AAGTGGCCGA TCTGCACCAA AAGCAGCGCG GGGAACCACA CTAACCACCC TCACATGGAT 
TGTGTCATAA CAGGCCGGCC TTGCTGCATC GGCACCAAGG GCAGGTGTGA GATCACCTCC 
CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACACT CTGCTCTCAG 
GTGCACTGCA TGGACGATGT CTGTGGCCTC CTGCCTTTCC TCAATCCTGA GGTGCCTGAC 
CAGTTCTACC GCCTGTGGCT GTCCCTCTTC CTGCACGCTG GGATCCTGCA CTGCTTGGTG 
TCCGTCTGCT TCCAGATGAC GGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC 
ATAGCCATCA TCTACCTGCT GAGTGGTATC ACGGGCAATC TAGCCAGTGC CATCTTCCTG 
CCATACCGGG CAGAGGTCCG GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG 
AGTTGTTCCA GAGCTGGCAG ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG 
CCGTGGTGCT CTTCCTCTTT GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA 
TCTCGGGCTT TGTCAGCGGC CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG 
GCAAGTTCGA CCTGTACCGC AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG 
GCCTGCTGGC CGGCCTGGTG GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG 
AGTTCCTCAC CTGCATCCCT TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC 
AGCTCCACTG AACCAGCCGT GGACTCCCCA GGCCAGCCCG GCCTCCCAGG GCTGAACAGA 
TGGGTGCTTC CTGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514566.1
CDS	565..2385
Translation	MSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514503.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514503.2

ORF Insert Sequence:

ATGTCCACGT ACCCAGATGA GGTGTTTGAG TCCCCATCAG AGGCAGCGCT CAAGGACTGG 
GAGAAAGCCC CGGATCAGGC CGACCTCACG GGTGGGGCCC TGGACCGCAG TGAGCTTGAG 
AGAAGCCACT TGATGTTGCC CCTGGAACGA GGCTGGAGAA AGCAGAAGGA AGGCGGCCCT 
CTAGCCCCGC AGCCCAAGGT GCGGCTTCGC CAGGAGGTGG TCAGTGCGGC GGGACCCAGG 
AGGGGTCAGC GTATCGCTGT GCCAGTGCGC AAACTCTTCG CTCGGGAAAA GCGGCCTTAT 
GGTCTGGGGA TGGTGGGACG GCTCACCAAC CGCACGTACC GGAAACGCAT CGACAGCTAT 
GTCAAGCGGC AGATCGAGGA CATGGATGAT CACAGGCCCT TCTTCACTTA CTGGCTCACC 
TTTGTGCACT CACTGGTCAC CATTCTGGCT GTGTGCATCT ATGGCATAGC GCCCGTGGGC 
TTCTCGCAGC ATGAGACGGT GGACTCGGTA CTTCGGAAGC GGGGTGTCTA TGAGAATGTC 
AAATACGTCC AGCAGGAGAA CTTCTGGATT GGCCCCAGCT CGGAGGCCCT CATTCACTTG 
GGTGCCAAGT TCTCACCCTG CATGCGTCAG GACCCACAGG TGCACAGCTT CATCCTGGCT 
GCCCGGGAGC GTGAAAAGCA CTCGGCTTGC TGTGTACGCA ATGATCGGTC TGGGTGTGTG 
CAGACTTCCA AGGAGGAGTG CTCGTCCACG TTGGCGGTGT GGGTGAAGTG GCCCGTTCAT 
CCTAGTGCCC CAGACCTTGC AGGCAACAAG AGACAGTTTG GTTCTGTCTG CCACCAGGAC 
CCCAGGGTAT GTGACGAGCC TTCCTCTGAG GATCCCCACG AGTGGCCAGA AGATATCACC 
AAGTGGCCGA TCTGCACCAA AAGCAGCGCG GGGAACCACA CTAACCACCC TCACATGGAT 
TGTGTCATAA CAGGCCGGCC TTGCTGCATC GGCACCAAGG GCAGGTGTGA GATCACCTCC 
CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACACT CTGCTCTCAG 
GTGCACTGCA TGGACGATGT CTGTGGCCTC CTGCCTTTCC TCAATCCTGA GGTGCCTGAC 
CAGTTCTACC GCCTGTGGCT GTCCCTCTTC CTGCACGCTG GGATCCTGCA CTGCTTGGTG 
TCCGTCTGCT TCCAGATGAC GGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC 
ATAGCCATCA TCTACCTGCT GAGTGGTATC ACGGGCAATC TAGCCAGTGC CATCTTCCTG 
CCATACCGGG CAGAGGTCCG GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG 
AGTTGTTCCA GAGCTGGCAG ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG 
CCGTGGTGCT CTTCCTCTTT GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA 
TCTCGGGCTT TGTCAGCGGC CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG 
GCAAGTTCGA CCTGTACCGC AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG 
GCCTGCTGGC CGGCCTGGTG GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG 
AGTTCCTCAC CTGCATCCCT TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC 
AGCTCCACTG AACCAGCCGT GGACTCCCCA GGCCAGCCCG GCCTCCCAGG GCTGAACAGA 
TGGGTGCTTC CTGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514494.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514557.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X3, mRNA.

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CloneID	OMu45066
Clone ID Related Accession (Same CDS sequence)	XM_006514494.4 , XM_006514494.3 , XM_006514496.3 , XM_006514495.3 , XM_006514495.4
Accession Version	XM_006514494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514557.1
CDS	190..2931
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514494.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514494.3

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514496.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514559.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu45066
Clone ID Related Accession (Same CDS sequence)	XM_006514494.4 , XM_006514494.3 , XM_006514496.3 , XM_006514495.3 , XM_006514495.4
Accession Version	XM_006514496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514559.1
CDS	49..2790
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514496.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514496.3

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514495.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514558.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X4, mRNA.

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CloneID	OMu45066
Clone ID Related Accession (Same CDS sequence)	XM_006514494.4 , XM_006514494.3 , XM_006514496.3 , XM_006514495.3 , XM_006514495.4
Accession Version	XM_006514495.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514558.1
CDS	255..2996
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514495.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514495.3

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514499.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514562.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu45069
Clone ID Related Accession (Same CDS sequence)	XM_006514499.3 , XM_006514499.4 , XM_030245523.1
Accession Version	XM_006514499.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTCCGGCCG 
GCTCCCAGTT TGGCATCCTG GCCTGCCTCT TTGTGGAGTT GTTCCAGAGC TGGCAGATCC 
TGGCCCGACC CTGGCGTGCC TTCTTCAAGC TCCTGGCCGT GGTGCTCTTC CTCTTTGCCT 
TTGGGCTGCT GCCCTGGATC GACAACTTCG CCCACATCTC GGGCTTTGTC AGCGGCCTCT 
TCCTTTCCTT TGCCTTCCTG CCTTACATCA GCTTTGGCAA GTTCGACCTG TACCGCAAGC 
GCTGCCAGAT CATCATCTTC CAGGTCGTCT TCCTGGGCCT GCTGGCCGGC CTGGTGGTCC 
TCTTCTACTT CTACCCGGTG CGCTGTGAAT GGTGCGAGTT CCTCACCTGC ATCCCTTTCA 
CGGACAAGTT CTGTGAAAAG TACGAGCTGG ACGCTCAGCT CCACTGAACC AGCCGTGGAC 
TCCCCAGGCC AGCCCGGCCT CCCAGGGCTG AACAGATGGG TGCTTCCTGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514562.1
CDS	188..2824
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514499.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514499.3

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTCCGGCCG 
GCTCCCAGTT TGGCATCCTG GCCTGCCTCT TTGTGGAGTT GTTCCAGAGC TGGCAGATCC 
TGGCCCGACC CTGGCGTGCC TTCTTCAAGC TCCTGGCCGT GGTGCTCTTC CTCTTTGCCT 
TTGGGCTGCT GCCCTGGATC GACAACTTCG CCCACATCTC GGGCTTTGTC AGCGGCCTCT 
TCCTTTCCTT TGCCTTCCTG CCTTACATCA GCTTTGGCAA GTTCGACCTG TACCGCAAGC 
GCTGCCAGAT CATCATCTTC CAGGTCGTCT TCCTGGGCCT GCTGGCCGGC CTGGTGGTCC 
TCTTCTACTT CTACCCGGTG CGCTGTGAAT GGTGCGAGTT CCTCACCTGC ATCCCTTTCA 
CGGACAAGTT CTGTGAAAAG TACGAGCTGG ACGCTCAGCT CCACTGAACC AGCCGTGGAC 
TCCCCAGGCC AGCCCGGCCT CCCAGGGCTG AACAGATGGG TGCTTCCTGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_011243657.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241959.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67606
Clone ID Related Accession (Same CDS sequence)	XM_011243657.2
Accession Version	XM_011243657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGCAAGTGG GACTTGGGGC TGGGCGTGTG GTCTCCTTCC CCAGGAGTGG TGGGCTGGAG 
GCAAAAGAGA AGCTCCCTTC TCTGCTGAGT GTCCCTCACT GCCCACAGGA TCCTGCACTG 
CTTGGTGTCC GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG 
GCACCGCATA GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT 
CTTCCTGCCA TACCGGGCAG AGGTAGGTCC GGCCGGCTCC CAGTTTGGCA TCCTGGCCTG 
CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC GTGCCTTCTT 
CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT GGATCGACAA 
CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT TCCTGCCTTA 
CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA TCTTCCAGGT 
CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC CGGTGCGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241959.1
CDS	46..2748
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGQVGLGAGRVVSFPRSGGLEAKEKLPSLLSVPHCPQDPALLGVRLLPDDGPAGPGEAGRLAPHSHHLPAEWYHGQSSQCHLPAIPGRGRSGRLPVWHPGLPLCGVVPELADPGPTLACLLQAPGRGALPLCLWAAALDRQLRPHLGLCQRPLPFLCLPALHQLWQVRPVPQALPDHHLPGRLPGPAGRPGGPLLLLPGAL

Target ORF information:

RefSeq Version	XM_011243657.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243657.2

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGCAAGTGG GACTTGGGGC TGGGCGTGTG GTCTCCTTCC CCAGGAGTGG TGGGCTGGAG 
GCAAAAGAGA AGCTCCCTTC TCTGCTGAGT GTCCCTCACT GCCCACAGGA TCCTGCACTG 
CTTGGTGTCC GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG 
GCACCGCATA GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT 
CTTCCTGCCA TACCGGGCAG AGGTAGGTCC GGCCGGCTCC CAGTTTGGCA TCCTGGCCTG 
CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC GTGCCTTCTT 
CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT GGATCGACAA 
CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT TCCTGCCTTA 
CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA TCTTCCAGGT 
CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC CGGTGCGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_011243658.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241960.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67607
Clone ID Related Accession (Same CDS sequence)	XM_011243658.2
Accession Version	XM_011243658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTAG GTCCGGCCGG CTCCCAGTTT 
GGCATCCTGG CCTGCCTCTT TGTGGAGTTG TTCCAGAGCT GGCAGATCCT GGCCCGACCC 
TGGCGTGCCT TCTTCAAGCT CCTGGCCGTG GTGCTCTTCC TCTTTGCCTT TGGGCTGCTG 
CCCTGGATCG ACAACTTCGC CCACATCTCG GGCTTTGTCA GCGGCCTCTT CCTTTCCTTT 
GCCTTCCTGC CTTACATCAG CTTTGGCAAG TTCGACCTGT ACCGCAAGCG CTGCCAGATC 
ATCATCTTCC AGGTCGTCTT CCTGGGCCTG CTGGCCGGCC TGGTGGTCCT CTTCTACTTC 
TACCCGGTGC GCTGTGAATG GTGCGAGTTC CTCACCTGCA TCCCTTTCAC GGACAAGTTC 
TGTGAAAAGT ACGAGCTGGA CGCTCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241960.1
CDS	46..2721
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDNFAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_011243658.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243658.2

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTAG GTCCGGCCGG CTCCCAGTTT 
GGCATCCTGG CCTGCCTCTT TGTGGAGTTG TTCCAGAGCT GGCAGATCCT GGCCCGACCC 
TGGCGTGCCT TCTTCAAGCT CCTGGCCGTG GTGCTCTTCC TCTTTGCCTT TGGGCTGCTG 
CCCTGGATCG ACAACTTCGC CCACATCTCG GGCTTTGTCA GCGGCCTCTT CCTTTCCTTT 
GCCTTCCTGC CTTACATCAG CTTTGGCAAG TTCGACCTGT ACCGCAAGCG CTGCCAGATC 
ATCATCTTCC AGGTCGTCTT CCTGGGCCTG CTGGCCGGCC TGGTGGTCCT CTTCTACTTC 
TACCCGGTGC GCTGTGAATG GTGCGAGTTC CTCACCTGCA TCCCTTTCAC GGACAAGTTC 
TGTGAAAAGT ACGAGCTGGA CGCTCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( NM_010117.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034247.2 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant 1, mRNA.

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CloneID	OMu19067
Clone ID Related Accession (Same CDS sequence)	XM_030245524.1 , NM_010117.2 , XM_030245525.1
Accession Version	NM_010117.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY270878.1, BY733688.1, CN664154.1, CD554689.1, AK144437.1 and AI842763.1. On Apr 8, 2009 this sequence version replaced NM_010117.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027346.1, SRR1660817.141302.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTAGGTCCG 
GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG AGTTGTTCCA GAGCTGGCAG 
ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG CCGTGGTGCT CTTCCTCTTT 
GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCGGGCTT TGTCAGCGGC 
CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG GCCTGCTGGC CGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG AGTTCCTCAC CTGCATCCCT 
TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034247.2
CDS	150..2720
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDNFAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	NM_010117.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010117.2

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTAGGTCCG 
GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG AGTTGTTCCA GAGCTGGCAG 
ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG CCGTGGTGCT CTTCCTCTTT 
GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCGGGCTT TGTCAGCGGC 
CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG GCCTGCTGGC CGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG AGTTCCTCAC CTGCATCCCT 
TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_030245524.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101384.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X7, mRNA.

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CloneID	OMu19067
Clone ID Related Accession (Same CDS sequence)	XM_030245524.1 , NM_010117.2 , XM_030245525.1
Accession Version	XM_030245524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTAGGTCCG 
GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG AGTTGTTCCA GAGCTGGCAG 
ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG CCGTGGTGCT CTTCCTCTTT 
GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCGGGCTT TGTCAGCGGC 
CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG GCCTGCTGGC CGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG AGTTCCTCAC CTGCATCCCT 
TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101384.1
CDS	250..2820
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDNFAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_030245524.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245524.1

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTAGGTCCG 
GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG AGTTGTTCCA GAGCTGGCAG 
ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG CCGTGGTGCT CTTCCTCTTT 
GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCGGGCTT TGTCAGCGGC 
CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG GCCTGCTGGC CGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG AGTTCCTCAC CTGCATCCCT 
TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_030245525.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101385.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X8, mRNA.

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CloneID	OMu19067
Clone ID Related Accession (Same CDS sequence)	XM_030245524.1 , NM_010117.2 , XM_030245525.1
Accession Version	XM_030245525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTAGGTCCG 
GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG AGTTGTTCCA GAGCTGGCAG 
ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG CCGTGGTGCT CTTCCTCTTT 
GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCGGGCTT TGTCAGCGGC 
CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG GCCTGCTGGC CGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG AGTTCCTCAC CTGCATCCCT 
TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101385.1
CDS	1788..4358
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDNFAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_030245525.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245525.1

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTAGGTCCG 
GCCGGCTCCC AGTTTGGCAT CCTGGCCTGC CTCTTTGTGG AGTTGTTCCA GAGCTGGCAG 
ATCCTGGCCC GACCCTGGCG TGCCTTCTTC AAGCTCCTGG CCGTGGTGCT CTTCCTCTTT 
GCCTTTGGGC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCGGGCTT TGTCAGCGGC 
CTCTTCCTTT CCTTTGCCTT CCTGCCTTAC ATCAGCTTTG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCAT CTTCCAGGTC GTCTTCCTGG GCCTGCTGGC CGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC GGTGCGCTGT GAATGGTGCG AGTTCCTCAC CTGCATCCCT 
TTCACGGACA AGTTCTGTGA AAAGTACGAG CTGGACGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514494.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514557.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45066
Clone ID Related Accession (Same CDS sequence)	XM_006514494.4 , XM_006514494.3 , XM_006514496.3 , XM_006514495.3 , XM_006514495.4
Accession Version	XM_006514494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514557.1
CDS	188..2929
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514494.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514494.4

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514495.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514558.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45066
Clone ID Related Accession (Same CDS sequence)	XM_006514494.4 , XM_006514494.3 , XM_006514496.3 , XM_006514495.3 , XM_006514495.4
Accession Version	XM_006514495.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514495.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514558.1
CDS	253..2994
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRSRQVRFGRVHTLPLLRPRAARKAHPQRQSLSWSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514495.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514495.4

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGCC GGCAGGTACG CTTTGGGCGT GTTCATACTC TGCCCCTTTT GAGGCCGAGG 
GCTGCTCGAA AGGCCCACCC ACAGCGCCAG TCTCTCTCAT GGTCCTTGCT CAGGGGGACA 
GCAGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGTCACT GCAGCCAGCG GTATGGGAAG CTAAAGCCGC AGGTCATCCG GGAGCTGGAC 
CTGCCCAGCC AGGACAATGT GTCCCTAACC AGCACCGAGA CACCACCTCC CCTCTACGTG 
GGGCCATGCC AGCTGGGCAT GCAGAAGATC ATAGACCCTC TGGCCCGAGG CCGGGCCTTT 
CGCATGGCTG ATGACACTGC TGATGGCCTG AGTGCCCCCC ACACTCCCGT CACACCAGGT 
GCAGCCTCCC TCTGCTCTTT CTCCAGCTCC CGCTCAGGTT TCAACCGACT CCCTCGGCGT 
CGCAAACGTG AATCGGTGGC CAAGATGAGC TTCCGGGCCG CTGCAGCATT GGTGAAGGGT 
CGGTCTATTA GGGATGGCAC TTTACGAAGG GGACAGCGCC GAAGCTTTAC CCCTGCCAGC 
TTTCTTGAGG AAGACATGGT GGACTTCCCT GATGAGTTGG ATACATCTTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GATGTCCACG TACCCAGATG AGGTGTTTGA GTCCCCATCA 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC CCGGATCAGG CCGACCTCAC GGGTGGGGCC 
CTGGACCGCA GTGAGCTTGA GAGAAGCCAC TTGATGTTGC CCCTGGAACG AGGCTGGAGA 
AAGCAGAAGG AAGGCGGCCC TCTAGCCCCG CAGCCCAAGG TGCGGCTTCG CCAGGAGGTG 
GTCAGTGCGG CGGGACCCAG GAGGGGTCAG CGTATCGCTG TGCCAGTGCG CAAACTCTTC 
GCTCGGGAAA AGCGGCCTTA TGGTCTGGGG ATGGTGGGAC GGCTCACCAA CCGCACGTAC 
CGGAAACGCA TCGACAGCTA TGTCAAGCGG CAGATCGAGG ACATGGATGA TCACAGGCCC 
TTCTTCACTT ACTGGCTCAC CTTTGTGCAC TCACTGGTCA CCATTCTGGC TGTGTGCATC 
TATGGCATAG CGCCCGTGGG CTTCTCGCAG CATGAGACGG TGGACTCGGT ACTTCGGAAG 
CGGGGTGTCT ATGAGAATGT CAAATACGTC CAGCAGGAGA ACTTCTGGAT TGGCCCCAGC 
TCGGAGGCCC TCATTCACTT GGGTGCCAAG TTCTCACCCT GCATGCGTCA GGACCCACAG 
GTGCACAGCT TCATCCTGGC TGCCCGGGAG CGTGAAAAGC ACTCGGCTTG CTGTGTACGC 
AATGATCGGT CTGGGTGTGT GCAGACTTCC AAGGAGGAGT GCTCGTCCAC GTTGGCGGTG 
TGGGTGAAGT GGCCCGTTCA TCCTAGTGCC CCAGACCTTG CAGGCAACAA GAGACAGTTT 
GGTTCTGTCT GCCACCAGGA CCCCAGGGTA TGTGACGAGC CTTCCTCTGA GGATCCCCAC 
GAGTGGCCAG AAGATATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGCGC GGGGAACCAC 
ACTAACCACC CTCACATGGA TTGTGTCATA ACAGGCCGGC CTTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCATGAG 
GAGGCCACAC TCTGCTCTCA GGTGCACTGC ATGGACGATG TCTGTGGCCT CCTGCCTTTC 
CTCAATCCTG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CCTGCACGCT 
GGGATCCTGC ACTGCTTGGT GTCCGTCTGC TTCCAGATGA CGGTCCTGCG GGACCTGGAG 
AAGCTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGC TGAGTGGTAT CACGGGCAAT 
CTAGCCAGTG CCATCTTCCT GCCATACCGG GCAGAGGTCC GGCCGGCTCC CAGTTTGGCA 
TCCTGGCCTG CCTCTTTGTG GAGTTGTTCC AGAGCTGGCA GATCCTGGCC CGACCCTGGC 
GTGCCTTCTT CAAGCTCCTG GCCGTGGTGC TCTTCCTCTT TGCCTTTGGG CTGCTGCCCT 
GGATCGACAA CTTCGCCCAC ATCTCGGGCT TTGTCAGCGG CCTCTTCCTT TCCTTTGCCT 
TCCTGCCTTA CATCAGCTTT GGCAAGTTCG ACCTGTACCG CAAGCGCTGC CAGATCATCA 
TCTTCCAGGT CGTCTTCCTG GGCCTGCTGG CCGGCCTGGT GGTCCTCTTC TACTTCTACC 
CGGTGCGCTG TGAATGGTGC GAGTTCCTCA CCTGCATCCC TTTCACGGAC AAGTTCTGTG 
AAAAGTACGA GCTGGACGCT CAGCTCCACT GAACCAGCCG TGGACTCCCC AGGCCAGCCC 
GGCCTCCCAG GGCTGAACAG ATGGGTGCTT CCTGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514499.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514562.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu45069
Clone ID Related Accession (Same CDS sequence)	XM_006514499.3 , XM_006514499.4 , XM_030245523.1
Accession Version	XM_006514499.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTCCGGCCG 
GCTCCCAGTT TGGCATCCTG GCCTGCCTCT TTGTGGAGTT GTTCCAGAGC TGGCAGATCC 
TGGCCCGACC CTGGCGTGCC TTCTTCAAGC TCCTGGCCGT GGTGCTCTTC CTCTTTGCCT 
TTGGGCTGCT GCCCTGGATC GACAACTTCG CCCACATCTC GGGCTTTGTC AGCGGCCTCT 
TCCTTTCCTT TGCCTTCCTG CCTTACATCA GCTTTGGCAA GTTCGACCTG TACCGCAAGC 
GCTGCCAGAT CATCATCTTC CAGGTCGTCT TCCTGGGCCT GCTGGCCGGC CTGGTGGTCC 
TCTTCTACTT CTACCCGGTG CGCTGTGAAT GGTGCGAGTT CCTCACCTGC ATCCCTTTCA 
CGGACAAGTT CTGTGAAAAG TACGAGCTGG ACGCTCAGCT CCACTGAACC AGCCGTGGAC 
TCCCCAGGCC AGCCCGGCCT CCCAGGGCTG AACAGATGGG TGCTTCCTGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514562.1
CDS	186..2822
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514499.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514499.4

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTCCGGCCG 
GCTCCCAGTT TGGCATCCTG GCCTGCCTCT TTGTGGAGTT GTTCCAGAGC TGGCAGATCC 
TGGCCCGACC CTGGCGTGCC TTCTTCAAGC TCCTGGCCGT GGTGCTCTTC CTCTTTGCCT 
TTGGGCTGCT GCCCTGGATC GACAACTTCG CCCACATCTC GGGCTTTGTC AGCGGCCTCT 
TCCTTTCCTT TGCCTTCCTG CCTTACATCA GCTTTGGCAA GTTCGACCTG TACCGCAAGC 
GCTGCCAGAT CATCATCTTC CAGGTCGTCT TCCTGGGCCT GCTGGCCGGC CTGGTGGTCC 
TCTTCTACTT CTACCCGGTG CGCTGTGAAT GGTGCGAGTT CCTCACCTGC ATCCCTTTCA 
CGGACAAGTT CTGTGAAAAG TACGAGCTGG ACGCTCAGCT CCACTGAACC AGCCGTGGAC 
TCCCCAGGCC AGCCCGGCCT CCCAGGGCTG AACAGATGGG TGCTTCCTGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_030245523.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101383.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X6, mRNA.

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CloneID	OMu45069
Clone ID Related Accession (Same CDS sequence)	XM_006514499.3 , XM_006514499.4 , XM_030245523.1
Accession Version	XM_030245523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTCCGGCCG 
GCTCCCAGTT TGGCATCCTG GCCTGCCTCT TTGTGGAGTT GTTCCAGAGC TGGCAGATCC 
TGGCCCGACC CTGGCGTGCC TTCTTCAAGC TCCTGGCCGT GGTGCTCTTC CTCTTTGCCT 
TTGGGCTGCT GCCCTGGATC GACAACTTCG CCCACATCTC GGGCTTTGTC AGCGGCCTCT 
TCCTTTCCTT TGCCTTCCTG CCTTACATCA GCTTTGGCAA GTTCGACCTG TACCGCAAGC 
GCTGCCAGAT CATCATCTTC CAGGTCGTCT TCCTGGGCCT GCTGGCCGGC CTGGTGGTCC 
TCTTCTACTT CTACCCGGTG CGCTGTGAAT GGTGCGAGTT CCTCACCTGC ATCCCTTTCA 
CGGACAAGTT CTGTGAAAAG TACGAGCTGG ACGCTCAGCT CCACTGAACC AGCCGTGGAC 
TCCCCAGGCC AGCCCGGCCT CCCAGGGCTG AACAGATGGG TGCTTCCTGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101383.1
CDS	251..2887
Translation	MSEARRDSTSSLQRKKPPWLKLDIPAAVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_030245523.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245523.1

ORF Insert Sequence:

ATGAGTGAAG CCAGGAGAGA CAGTACTAGC AGCCTGCAAC GCAAGAAACC CCCATGGCTC 
AAGTTGGACA TCCCAGCCGC GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
CTGCGGCGAC AGGCTTTCTT GCGGAGTGTG AGTATGCCAG CTGAGACAGC CCGGGTCCCA 
TCACCCCACC ATGAGCCTCG CCGGCTGGTG CTGCAGCGTC AGACATCCAT CACACAGACC 
ATCCGCAGGG GGACAGCAGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGGTATG GGAAGCTAAA GCCGCAGGTC 
ATCCGGGAGC TGGACCTGCC CAGCCAGGAC AATGTGTCCC TAACCAGCAC CGAGACACCA 
CCTCCCCTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCTCTGGCC 
CGAGGCCGGG CCTTTCGCAT GGCTGATGAC ACTGCTGATG GCCTGAGTGC CCCCCACACT 
CCCGTCACAC CAGGTGCAGC CTCCCTCTGC TCTTTCTCCA GCTCCCGCTC AGGTTTCAAC 
CGACTCCCTC GGCGTCGCAA ACGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCCGCTGCA 
GCATTGGTGA AGGGTCGGTC TATTAGGGAT GGCACTTTAC GAAGGGGACA GCGCCGAAGC 
TTTACCCCTG CCAGCTTTCT TGAGGAAGAC ATGGTGGACT TCCCTGATGA GTTGGATACA 
TCTTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGATGT CCACGTACCC AGATGAGGTG 
TTTGAGTCCC CATCAGAGGC AGCGCTCAAG GACTGGGAGA AAGCCCCGGA TCAGGCCGAC 
CTCACGGGTG GGGCCCTGGA CCGCAGTGAG CTTGAGAGAA GCCACTTGAT GTTGCCCCTG 
GAACGAGGCT GGAGAAAGCA GAAGGAAGGC GGCCCTCTAG CCCCGCAGCC CAAGGTGCGG 
CTTCGCCAGG AGGTGGTCAG TGCGGCGGGA CCCAGGAGGG GTCAGCGTAT CGCTGTGCCA 
GTGCGCAAAC TCTTCGCTCG GGAAAAGCGG CCTTATGGTC TGGGGATGGT GGGACGGCTC 
ACCAACCGCA CGTACCGGAA ACGCATCGAC AGCTATGTCA AGCGGCAGAT CGAGGACATG 
GATGATCACA GGCCCTTCTT CACTTACTGG CTCACCTTTG TGCACTCACT GGTCACCATT 
CTGGCTGTGT GCATCTATGG CATAGCGCCC GTGGGCTTCT CGCAGCATGA GACGGTGGAC 
TCGGTACTTC GGAAGCGGGG TGTCTATGAG AATGTCAAAT ACGTCCAGCA GGAGAACTTC 
TGGATTGGCC CCAGCTCGGA GGCCCTCATT CACTTGGGTG CCAAGTTCTC ACCCTGCATG 
CGTCAGGACC CACAGGTGCA CAGCTTCATC CTGGCTGCCC GGGAGCGTGA AAAGCACTCG 
GCTTGCTGTG TACGCAATGA TCGGTCTGGG TGTGTGCAGA CTTCCAAGGA GGAGTGCTCG 
TCCACGTTGG CGGTGTGGGT GAAGTGGCCC GTTCATCCTA GTGCCCCAGA CCTTGCAGGC 
AACAAGAGAC AGTTTGGTTC TGTCTGCCAC CAGGACCCCA GGGTATGTGA CGAGCCTTCC 
TCTGAGGATC CCCACGAGTG GCCAGAAGAT ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGCGCGGGGA ACCACACTAA CCACCCTCAC ATGGATTGTG TCATAACAGG CCGGCCTTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ATGAGGAGGC CACACTCTGC TCTCAGGTGC ACTGCATGGA CGATGTCTGT 
GGCCTCCTGC CTTTCCTCAA TCCTGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCCTGC ACGCTGGGAT CCTGCACTGC TTGGTGTCCG TCTGCTTCCA GATGACGGTC 
CTGCGGGACC TGGAGAAGCT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGCTGAGT 
GGTATCACGG GCAATCTAGC CAGTGCCATC TTCCTGCCAT ACCGGGCAGA GGTCCGGCCG 
GCTCCCAGTT TGGCATCCTG GCCTGCCTCT TTGTGGAGTT GTTCCAGAGC TGGCAGATCC 
TGGCCCGACC CTGGCGTGCC TTCTTCAAGC TCCTGGCCGT GGTGCTCTTC CTCTTTGCCT 
TTGGGCTGCT GCCCTGGATC GACAACTTCG CCCACATCTC GGGCTTTGTC AGCGGCCTCT 
TCCTTTCCTT TGCCTTCCTG CCTTACATCA GCTTTGGCAA GTTCGACCTG TACCGCAAGC 
GCTGCCAGAT CATCATCTTC CAGGTCGTCT TCCTGGGCCT GCTGGCCGGC CTGGTGGTCC 
TCTTCTACTT CTACCCGGTG CGCTGTGAAT GGTGCGAGTT CCTCACCTGC ATCCCTTTCA 
CGGACAAGTT CTGTGAAAAG TACGAGCTGG ACGCTCAGCT CCACTGAACC AGCCGTGGAC 
TCCCCAGGCC AGCCCGGCCT CCCAGGGCTG AACAGATGGG TGCTTCCTGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_006514501.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514564.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu45071
Clone ID Related Accession (Same CDS sequence)	XM_006514501.3 , XM_006514501.4
Accession Version	XM_006514501.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514501.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC GGGCCGCTGC AGCATTGGTG AAGGGTCGGT CTATTAGGGA TGGCACTTTA 
CGAAGGGGAC AGCGCCGAAG CTTTACCCCT GCCAGCTTTC TTGAGGAAGA CATGGTGGAC 
TTCCCTGATG AGTTGGATAC ATCTTTCTTT GCTCGGGAAG GTGTCCTCCA CGAGGAGATG 
TCCACGTACC CAGATGAGGT GTTTGAGTCC CCATCAGAGG CAGCGCTCAA GGACTGGGAG 
AAAGCCCCGG ATCAGGCCGA CCTCACGGGT GGGGCCCTGG ACCGCAGTGA GCTTGAGAGA 
AGCCACTTGA TGTTGCCCCT GGAACGAGGC TGGAGAAAGC AGAAGGAAGG CGGCCCTCTA 
GCCCCGCAGC CCAAGGTGCG GCTTCGCCAG GAGGTGGTCA GTGCGGCGGG ACCCAGGAGG 
GGTCAGCGTA TCGCTGTGCC AGTGCGCAAA CTCTTCGCTC GGGAAAAGCG GCCTTATGGT 
CTGGGGATGG TGGGACGGCT CACCAACCGC ACGTACCGGA AACGCATCGA CAGCTATGTC 
AAGCGGCAGA TCGAGGACAT GGATGATCAC AGGCCCTTCT TCACTTACTG GCTCACCTTT 
GTGCACTCAC TGGTCACCAT TCTGGCTGTG TGCATCTATG GCATAGCGCC CGTGGGCTTC 
TCGCAGCATG AGACGGTGGA CTCGGTACTT CGGAAGCGGG GTGTCTATGA GAATGTCAAA 
TACGTCCAGC AGGAGAACTT CTGGATTGGC CCCAGCTCGG AGGCCCTCAT TCACTTGGGT 
GCCAAGTTCT CACCCTGCAT GCGTCAGGAC CCACAGGTGC ACAGCTTCAT CCTGGCTGCC 
CGGGAGCGTG AAAAGCACTC GGCTTGCTGT GTACGCAATG ATCGGTCTGG GTGTGTGCAG 
ACTTCCAAGG AGGAGTGCTC GTCCACGTTG GCGGTGTGGG TGAAGTGGCC CGTTCATCCT 
AGTGCCCCAG ACCTTGCAGG CAACAAGAGA CAGTTTGGTT CTGTCTGCCA CCAGGACCCC 
AGGGTATGTG ACGAGCCTTC CTCTGAGGAT CCCCACGAGT GGCCAGAAGA TATCACCAAG 
TGGCCGATCT GCACCAAAAG CAGCGCGGGG AACCACACTA ACCACCCTCA CATGGATTGT 
GTCATAACAG GCCGGCCTTG CTGCATCGGC ACCAAGGGCA GGTGTGAGAT CACCTCCCGG 
GAGTACTGTG ACTTCATGAG GGGCTACTTC CATGAGGAGG CCACACTCTG CTCTCAGGTG 
CACTGCATGG ACGATGTCTG TGGCCTCCTG CCTTTCCTCA ATCCTGAGGT GCCTGACCAG 
TTCTACCGCC TGTGGCTGTC CCTCTTCCTG CACGCTGGGA TCCTGCACTG CTTGGTGTCC 
GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG GCACCGCATA 
GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT CTTCCTGCCA 
TACCGGGCAG AGGTCCGGCC GGCTCCCAGT TTGGCATCCT GGCCTGCCTC TTTGTGGAGT 
TGTTCCAGAG CTGGCAGATC CTGGCCCGAC CCTGGCGTGC CTTCTTCAAG CTCCTGGCCG 
TGGTGCTCTT CCTCTTTGCC TTTGGGCTGC TGCCCTGGAT CGACAACTTC GCCCACATCT 
CGGGCTTTGT CAGCGGCCTC TTCCTTTCCT TTGCCTTCCT GCCTTACATC AGCTTTGGCA 
AGTTCGACCT GTACCGCAAG CGCTGCCAGA TCATCATCTT CCAGGTCGTC TTCCTGGGCC 
TGCTGGCCGG CCTGGTGGTC CTCTTCTACT TCTACCCGGT GCGCTGTGAA TGGTGCGAGT 
TCCTCACCTG CATCCCTTTC ACGGACAAGT TCTGTGAAAA GTACGAGCTG GACGCTCAGC 
TCCACTGAAC CAGCCGTGGA CTCCCCAGGC CAGCCCGGCC TCCCAGGGCT GAACAGATGG 
GTGCTTCCTG GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514564.1
CDS	707..2704
Translation	MSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEEMSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVRPAPSLASWPASLWSCSRAGRSWPDPGVPSSSSWPWCSSSLPLGCCPGSTTSPTSRALSAASSFPLPSCLTSALASSTCTASAARSSSSRSSSWACWPAWWSSSTSTRCAVNGASSSPASLSRTSSVKSTSWTLSSTEPAVDSPGQPGLPGLNRWVLPGH

Target ORF information:

RefSeq Version	XM_006514501.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514501.4

ORF Insert Sequence:

ATGAGCTTCC GGGCCGCTGC AGCATTGGTG AAGGGTCGGT CTATTAGGGA TGGCACTTTA 
CGAAGGGGAC AGCGCCGAAG CTTTACCCCT GCCAGCTTTC TTGAGGAAGA CATGGTGGAC 
TTCCCTGATG AGTTGGATAC ATCTTTCTTT GCTCGGGAAG GTGTCCTCCA CGAGGAGATG 
TCCACGTACC CAGATGAGGT GTTTGAGTCC CCATCAGAGG CAGCGCTCAA GGACTGGGAG 
AAAGCCCCGG ATCAGGCCGA CCTCACGGGT GGGGCCCTGG ACCGCAGTGA GCTTGAGAGA 
AGCCACTTGA TGTTGCCCCT GGAACGAGGC TGGAGAAAGC AGAAGGAAGG CGGCCCTCTA 
GCCCCGCAGC CCAAGGTGCG GCTTCGCCAG GAGGTGGTCA GTGCGGCGGG ACCCAGGAGG 
GGTCAGCGTA TCGCTGTGCC AGTGCGCAAA CTCTTCGCTC GGGAAAAGCG GCCTTATGGT 
CTGGGGATGG TGGGACGGCT CACCAACCGC ACGTACCGGA AACGCATCGA CAGCTATGTC 
AAGCGGCAGA TCGAGGACAT GGATGATCAC AGGCCCTTCT TCACTTACTG GCTCACCTTT 
GTGCACTCAC TGGTCACCAT TCTGGCTGTG TGCATCTATG GCATAGCGCC CGTGGGCTTC 
TCGCAGCATG AGACGGTGGA CTCGGTACTT CGGAAGCGGG GTGTCTATGA GAATGTCAAA 
TACGTCCAGC AGGAGAACTT CTGGATTGGC CCCAGCTCGG AGGCCCTCAT TCACTTGGGT 
GCCAAGTTCT CACCCTGCAT GCGTCAGGAC CCACAGGTGC ACAGCTTCAT CCTGGCTGCC 
CGGGAGCGTG AAAAGCACTC GGCTTGCTGT GTACGCAATG ATCGGTCTGG GTGTGTGCAG 
ACTTCCAAGG AGGAGTGCTC GTCCACGTTG GCGGTGTGGG TGAAGTGGCC CGTTCATCCT 
AGTGCCCCAG ACCTTGCAGG CAACAAGAGA CAGTTTGGTT CTGTCTGCCA CCAGGACCCC 
AGGGTATGTG ACGAGCCTTC CTCTGAGGAT CCCCACGAGT GGCCAGAAGA TATCACCAAG 
TGGCCGATCT GCACCAAAAG CAGCGCGGGG AACCACACTA ACCACCCTCA CATGGATTGT 
GTCATAACAG GCCGGCCTTG CTGCATCGGC ACCAAGGGCA GGTGTGAGAT CACCTCCCGG 
GAGTACTGTG ACTTCATGAG GGGCTACTTC CATGAGGAGG CCACACTCTG CTCTCAGGTG 
CACTGCATGG ACGATGTCTG TGGCCTCCTG CCTTTCCTCA ATCCTGAGGT GCCTGACCAG 
TTCTACCGCC TGTGGCTGTC CCTCTTCCTG CACGCTGGGA TCCTGCACTG CTTGGTGTCC 
GTCTGCTTCC AGATGACGGT CCTGCGGGAC CTGGAGAAGC TGGCAGGCTG GCACCGCATA 
GCCATCATCT ACCTGCTGAG TGGTATCACG GGCAATCTAG CCAGTGCCAT CTTCCTGCCA 
TACCGGGCAG AGGTCCGGCC GGCTCCCAGT TTGGCATCCT GGCCTGCCTC TTTGTGGAGT 
TGTTCCAGAG CTGGCAGATC CTGGCCCGAC CCTGGCGTGC CTTCTTCAAG CTCCTGGCCG 
TGGTGCTCTT CCTCTTTGCC TTTGGGCTGC TGCCCTGGAT CGACAACTTC GCCCACATCT 
CGGGCTTTGT CAGCGGCCTC TTCCTTTCCT TTGCCTTCCT GCCTTACATC AGCTTTGGCA 
AGTTCGACCT GTACCGCAAG CGCTGCCAGA TCATCATCTT CCAGGTCGTC TTCCTGGGCC 
TGCTGGCCGG CCTGGTGGTC CTCTTCTACT TCTACCCGGT GCGCTGTGAA TGGTGCGAGT 
TCCTCACCTG CATCCCTTTC ACGGACAAGT TCTGTGAAAA GTACGAGCTG GACGCTCAGC 
TCCACTGAAC CAGCCGTGGA CTCCCCAGGC CAGCCCGGCC TCCCAGGGCT GAACAGATGG 
GTGCTTCCTG GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhbdf1 ( XM_030245526.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101386.1 Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X13, mRNA.

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CloneID	OMu85618
Clone ID Related Accession (Same CDS sequence)	XM_030245526.1
Accession Version	XM_030245526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inactive rhomboid protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACGT ACCCAGATGA GGTGTTTGAG TCCCCATCAG AGGCAGCGCT CAAGGACTGG 
GAGAAAGCCC CGGATCAGGC CGACCTCACG GGTGGGGCCC TGGACCGCAG TGAGCTTGAG 
AGAAGCCACT TGATGTTGCC CCTGGAACGA GGCTGGAGAA AGCAGAAGGA AGGCGGCCCT 
CTAGCCCCGC AGCCCAAGGT GCGGCTTCGC CAGGAGGTGG TCAGTGCGGC GGGACCCAGG 
AGGGGTCAGC GTATCGCTGT GCCAGTGCGC AAACTCTTCG CTCGGGAAAA GCGGCCTTAT 
GGTCTGGGGA TGGTGGGACG GCTCACCAAC CGCACGTACC GGAAACGCAT CGACAGCTAT 
GTCAAGCGGC AGATCGAGGA CATGGATGAT CACAGGCCCT TCTTCACTTA CTGGCTCACC 
TTTGTGCACT CACTGGTCAC CATTCTGGCT GTGTGCATCT ATGGCATAGC GCCCGTGGGC 
TTCTCGCAGC ATGAGACGGT GGACTCGGTA CTTCGGAAGC GGGGTGTCTA TGAGAATGTC 
AAATACGTCC AGCAGGAGAA CTTCTGGATT GGCCCCAGCT CGGAGGCCCT CATTCACTTG 
GGTGCCAAGT TCTCACCCTG CATGCGTCAG GACCCACAGG TGCACAGCTT CATCCTGGCT 
GCCCGGGAGC GTGAAAAGCA CTCGGCTTGC TGTGTACGCA ATGATCGGTC TGGGTGTGTG 
CAGACTTCCA AGGAGGAGTG CTCGTCCACG TTGGCGGTGT GGGTGAAGTG GCCCGTTCAT 
CCTAGTGCCC CAGACCTTGC AGGCAACAAG AGACAGTTTG GTTCTGTCTG CCACCAGGAC 
CCCAGGGTAT GTGACGAGCC TTCCTCTGAG GATCCCCACG AGTGGCCAGA AGATATCACC 
AAGTGGCCGA TCTGCACCAA AAGCAGCGCG GGGAACCACA CTAACCACCC TCACATGGAT 
TGTGTCATAA CAGGCCGGCC TTGCTGCATC GGCACCAAGG GCAGGTGTGA GATCACCTCC 
CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACACT CTGCTCTCAG 
GTGCACTGCA TGGACGATGT CTGTGGCCTC CTGCCTTTCC TCAATCCTGA GGTGCCTGAC 
CAGTTCTACC GCCTGTGGCT GTCCCTCTTC CTGCACGCTG GGATCCTGCA CTGCTTGGTG 
TCCGTCTGCT TCCAGATGAC GGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC 
ATAGCCATCA TCTACCTGCT GAGTGGTATC ACGGGCAATC TAGCCAGTGC CATCTTCCTG 
CCATACCGGG CAGAGGTAGG TCCGGCCGGC TCCCAGTTTG GCATCCTGGC CTGCCTCTTT 
GTGGAGTTGT TCCAGAGCTG GCAGATCCTG GCCCGACCCT GGCGTGCCTT CTTCAAGCTC 
CTGGCCGTGG TGCTCTTCCT CTTTGCCTTT GGGCTGCTGC CCTGGATCGA CAACTTCGCC 
CACATCTCGG GCTTTGTCAG CGGCCTCTTC CTTTCCTTTG CCTTCCTGCC TTACATCAGC 
TTTGGCAAGT TCGACCTGTA CCGCAAGCGC TGCCAGATCA TCATCTTCCA GGTCGTCTTC 
CTGGGCCTGC TGGCCGGCCT GGTGGTCCTC TTCTACTTCT ACCCGGTGCG CTGTGAATGG 
TGCGAGTTCC TCACCTGCAT CCCTTTCACG GACAAGTTCT GTGAAAAGTA CGAGCTGGAC 
GCTCAGCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101386.1
CDS	997..2751
Translation	MSTYPDEVFESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGPLAPQPKVRLRQEVVSAAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFILAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDNFAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_030245526.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rhomboid 5 homolog 1 (Rhbdf1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245526.1

ORF Insert Sequence:

ATGTCCACGT ACCCAGATGA GGTGTTTGAG TCCCCATCAG AGGCAGCGCT CAAGGACTGG 
GAGAAAGCCC CGGATCAGGC CGACCTCACG GGTGGGGCCC TGGACCGCAG TGAGCTTGAG 
AGAAGCCACT TGATGTTGCC CCTGGAACGA GGCTGGAGAA AGCAGAAGGA AGGCGGCCCT 
CTAGCCCCGC AGCCCAAGGT GCGGCTTCGC CAGGAGGTGG TCAGTGCGGC GGGACCCAGG 
AGGGGTCAGC GTATCGCTGT GCCAGTGCGC AAACTCTTCG CTCGGGAAAA GCGGCCTTAT 
GGTCTGGGGA TGGTGGGACG GCTCACCAAC CGCACGTACC GGAAACGCAT CGACAGCTAT 
GTCAAGCGGC AGATCGAGGA CATGGATGAT CACAGGCCCT TCTTCACTTA CTGGCTCACC 
TTTGTGCACT CACTGGTCAC CATTCTGGCT GTGTGCATCT ATGGCATAGC GCCCGTGGGC 
TTCTCGCAGC ATGAGACGGT GGACTCGGTA CTTCGGAAGC GGGGTGTCTA TGAGAATGTC 
AAATACGTCC AGCAGGAGAA CTTCTGGATT GGCCCCAGCT CGGAGGCCCT CATTCACTTG 
GGTGCCAAGT TCTCACCCTG CATGCGTCAG GACCCACAGG TGCACAGCTT CATCCTGGCT 
GCCCGGGAGC GTGAAAAGCA CTCGGCTTGC TGTGTACGCA ATGATCGGTC TGGGTGTGTG 
CAGACTTCCA AGGAGGAGTG CTCGTCCACG TTGGCGGTGT GGGTGAAGTG GCCCGTTCAT 
CCTAGTGCCC CAGACCTTGC AGGCAACAAG AGACAGTTTG GTTCTGTCTG CCACCAGGAC 
CCCAGGGTAT GTGACGAGCC TTCCTCTGAG GATCCCCACG AGTGGCCAGA AGATATCACC 
AAGTGGCCGA TCTGCACCAA AAGCAGCGCG GGGAACCACA CTAACCACCC TCACATGGAT 
TGTGTCATAA CAGGCCGGCC TTGCTGCATC GGCACCAAGG GCAGGTGTGA GATCACCTCC 
CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACACT CTGCTCTCAG 
GTGCACTGCA TGGACGATGT CTGTGGCCTC CTGCCTTTCC TCAATCCTGA GGTGCCTGAC 
CAGTTCTACC GCCTGTGGCT GTCCCTCTTC CTGCACGCTG GGATCCTGCA CTGCTTGGTG 
TCCGTCTGCT TCCAGATGAC GGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC 
ATAGCCATCA TCTACCTGCT GAGTGGTATC ACGGGCAATC TAGCCAGTGC CATCTTCCTG 
CCATACCGGG CAGAGGTAGG TCCGGCCGGC TCCCAGTTTG GCATCCTGGC CTGCCTCTTT 
GTGGAGTTGT TCCAGAGCTG GCAGATCCTG GCCCGACCCT GGCGTGCCTT CTTCAAGCTC 
CTGGCCGTGG TGCTCTTCCT CTTTGCCTTT GGGCTGCTGC CCTGGATCGA CAACTTCGCC 
CACATCTCGG GCTTTGTCAG CGGCCTCTTC CTTTCCTTTG CCTTCCTGCC TTACATCAGC 
TTTGGCAAGT TCGACCTGTA CCGCAAGCGC TGCCAGATCA TCATCTTCCA GGTCGTCTTC 
CTGGGCCTGC TGGCCGGCCT GGTGGTCCTC TTCTACTTCT ACCCGGTGCG CTGTGAATGG 
TGCGAGTTCC TCACCTGCAT CCCTTTCACG GACAAGTTCT GTGAAAAGTA CGAGCTGGAC 
GCTCAGCTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Rhbdf1
Entrez Gene ID	13650
Full Name	rhomboid 5 homolog 1
Synonyms	C16ORF8,Dist,Dist1,Egfr-rs,mKIAA4242
General protein information	Preferred Names rhomboid 5 homolog 1 Names inactive rhomboid protein 1 epidermal growth factor receptor related sequence epidermal growth factor receptor-related protein iRhom1 rhomboid family 1 rhomboid family member 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 A4\|11 18.83 cM

Pan troglodytes (chimpanzee)	RHBDF1	XP_510701.3
Rattus norvegicus (Norway rat)	Rhbdf1	NP_001025205.1
Danio rerio (zebrafish)	rhbdf1	NP_001002228.1
Xenopus tropicalis (tropical clawed frog)	rhbdf1	XP_004917998.1
Homo sapiens (human)	RHBDF1	NP_071895.3
Gallus gallus (chicken)	RHBDF1	XP_004945406.1
Canis lupus familiaris (dog)	RHBDF1	XP_005621827.1
Bos taurus (cattle)	RHBDF1	NP_001098870.1
Mus musculus (house mouse)	Rhbdf1	NP_034247.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Rhbdf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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