Dffa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dffa
Entrez Gene ID	13347
Full Name	DNA fragmentation factor, alpha subunit
Synonyms	A330085O09Rik,DFF35,Dff45,ICAD,ICAD-L,ICAD-S
General protein information	Preferred Names DNA fragmentation factor, alpha subunit Names DNA fragmentation factor subunit alpha DNA fragmentation factor 45 kDa subunit inhibitor of CAD
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_010044.3	NP_034174.2	DNA fragmentation factor subunit alpha isoform b
NM_001025296.2	NP_001020467.1	DNA fragmentation factor subunit alpha isoform a

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dffa	NP_446131.2
Gallus gallus (chicken)	DFFA	XP_417610.1
Bos taurus (cattle)	DFFA	NP_001068810.1
	DFFA	XP_001102195.1
Canis lupus familiaris (dog)	DFFA	XP_544574.2
Danio rerio (zebrafish)	dffa	NP_001002631.1
Homo sapiens (human)	DFFA	NP_004392.1
Pan troglodytes (chimpanzee)	DFFA	XP_001161941.1
Mus musculus (house mouse)	Dffa	NP_001020467.1
Xenopus tropicalis (tropical clawed frog)	dffa	XP_002939454.2

Gene Ontology
Bibliography

Related articles in PubMed

ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability.
Errami Y, Brim H, Oumouna-Benachour K, Oumouna M, Naura AS, Kim H, Ju J, Davis CJ, Kim JG, Ashktorab H, Fallon K, Xu M, Zhang J, Del Valle L, Boulares AH
PloS one8(2)e57871(2013)

Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase.
Kutscher D, Pingoud A, Jeltsch A, Meiss G
The FEBS journal279(16)2917-28(2012 Aug)

Engineered apoptotic nucleases for chromatin research.
Xiao F, Widlak P, Garrard WT
Nucleic acids research35(13)e93(2007)

A unique role of the DNA fragmentation factor in maintaining genomic stability.
Yan B, Wang H, Peng Y, Hu Y, Wang H, Zhang X, Chen Q, Bedford JS, Dewhirst MW, Li CY
Proceedings of the National Academy of Sciences of the United States of America103(5)1504-9(2006 Jan)

Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
Korn C, Scholz SR, Gimadutdinow O, Lurz R, Pingoud A, Meiss G
The Journal of biological chemistry280(7)6005-15(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

apoptotic DNA fragmentation factor is required for the maintenance of genetic stability and may play a role in tumor suppression
Title: A unique role of the DNA fragmentation factor in maintaining genomic stability.

a lack of DFF45 facilitates hippocampus-dependent nonspatial memory, as well as hippocampus-dependent spatial memory
Title: DNA fragmentation factor 45 knockout mice exhibit longer memory retention in the novel object recognition task compared to wild-type mice.

Co-transfection of mouse DFF45(-/-) fibroblasts with plasmids encoding human DFF40 and DFF45 reversed the apoptosis resistance normally observed in these cells
Title: Roles of DNA fragmentation factor and poly(ADP-ribose) polymerase-1 in sensitization of fibroblasts to tumor necrosis factor-induced apoptosis.

The following Dffa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dffa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05429	NM_010044.3 Latest version!	Mus musculus DNA fragmentation factor, alpha subunit (Dffa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu12086	NM_001025296.2 Latest version!	Mus musculus DNA fragmentation factor, alpha subunit (Dffa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dffa ( NM_010044.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034174.2 Mus musculus DNA fragmentation factor, alpha subunit (Dffa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05429
Clone ID Related Accession (Same CDS sequence)	NM_010044.3
Accession Version	NM_010044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	DNA fragmentation factor subunit alpha isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY170336.1, BY208552.1 and AK079634.1. On Sep 24, 2013 this sequence version replaced NM_010044.2. Transcript Variant: This variant (2) contains an alternate 3' terminal exon, resulting in a different 3' UTR and 3' coding region, compared to variant 1. It encodes isoform b, which has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTGT CGCGGGGAGC CAGCGCCCCA GACCCGGACG ATGTCCGGCC TCTCAAACCG 
TGTCTGCTAC GCCGCAACCA CAGCCGCGAT CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTCCTG GCCATTGATA AGTCCCTGAC GCCGATCACC 
CTGGTCCTGG CTGAGGACGG GACCATAGTG GATGACGATG ACTACTTCCT CTGCCTTCCT 
TCCAATACGA AGTTTGTGGC GTTGGCCTGC AATGAGAAGT GGATTTATAA TGATTCCGAT 
GGAGGGACGG CTTGGGTTTC CCAAGAGTCC TTTGAGGCAG ATGAGCCGGA CAGCAGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGACCTCCA AGCGCTCATC GACATCCCAT GTGCAGAGCT GGCTCAGGAA 
CTCTGCCAAA GCTGTGCCAC TGTCCAGGGG CTGCAGAGCA CACTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCCCGCCA GTCCAAGCAG CTCCTGGAAC TTTACCTCCA GGCCTTGGAG 
AAAGAGGGCA ACATCTTGTC CAACCAGAAA GAGTCCAAAG CTGCCCTCAG TGAAGAGCTG 
GATGCAGTTG ACACAGGCGT CGGCAGAGAG ATGGCTTCGG AAGTGCTGCT CAGAAGCCAG 
ATCCTTACCA CACTGAAGGA GAAGCCTGCC CCAGAGCTGA GTTTATCTAG TCAGGATTTG 
GAGGTGGGCA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034174.2
CDS	110..907
Translation	MELSRGASAPDPDDVRPLKPCLLRRNHSRDQHGVAASSLEELRSKACELLAIDKSLTPITLVLAEDGTIVDDDDYFLCLPSNTKFVALACNEKWIYNDSDGGTAWVSQESFEADEPDSRAGVKWKNVARQLKEDLSSIILLSEEDLQALIDIPCAELAQELCQSCATVQGLQSTLQQVLDQREEARQSKQLLELYLQALEKEGNILSNQKESKAALSEELDAVDTGVGREMASEVLLRSQILTTLKEKPAPELSLSSQDLEVGKN

Target ORF information:

RefSeq Version	NM_010044.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA fragmentation factor, alpha subunit (Dffa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010044.3

ORF Insert Sequence:

ATGGAGCTGT CGCGGGGAGC CAGCGCCCCA GACCCGGACG ATGTCCGGCC TCTCAAACCG 
TGTCTGCTAC GCCGCAACCA CAGCCGCGAT CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTCCTG GCCATTGATA AGTCCCTGAC GCCGATCACC 
CTGGTCCTGG CTGAGGACGG GACCATAGTG GATGACGATG ACTACTTCCT CTGCCTTCCT 
TCCAATACGA AGTTTGTGGC GTTGGCCTGC AATGAGAAGT GGATTTATAA TGATTCCGAT 
GGAGGGACGG CTTGGGTTTC CCAAGAGTCC TTTGAGGCAG ATGAGCCGGA CAGCAGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGACCTCCA AGCGCTCATC GACATCCCAT GTGCAGAGCT GGCTCAGGAA 
CTCTGCCAAA GCTGTGCCAC TGTCCAGGGG CTGCAGAGCA CACTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCCCGCCA GTCCAAGCAG CTCCTGGAAC TTTACCTCCA GGCCTTGGAG 
AAAGAGGGCA ACATCTTGTC CAACCAGAAA GAGTCCAAAG CTGCCCTCAG TGAAGAGCTG 
GATGCAGTTG ACACAGGCGT CGGCAGAGAG ATGGCTTCGG AAGTGCTGCT CAGAAGCCAG 
ATCCTTACCA CACTGAAGGA GAAGCCTGCC CCAGAGCTGA GTTTATCTAG TCAGGATTTG 
GAGGTGGGCA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dffa ( NM_001025296.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001020467.1 Mus musculus DNA fragmentation factor, alpha subunit (Dffa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12086
Clone ID Related Accession (Same CDS sequence)	NM_001025296.2
Accession Version	NM_001025296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	DNA fragmentation factor subunit alpha isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY170336.1, BY208552.1, AK051011.1, AA512775.1 and AI956986.1. On Sep 24, 2013 this sequence version replaced NM_001025296.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTGT CGCGGGGAGC CAGCGCCCCA GACCCGGACG ATGTCCGGCC TCTCAAACCG 
TGTCTGCTAC GCCGCAACCA CAGCCGCGAT CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTCCTG GCCATTGATA AGTCCCTGAC GCCGATCACC 
CTGGTCCTGG CTGAGGACGG GACCATAGTG GATGACGATG ACTACTTCCT CTGCCTTCCT 
TCCAATACGA AGTTTGTGGC GTTGGCCTGC AATGAGAAGT GGATTTATAA TGATTCCGAT 
GGAGGGACGG CTTGGGTTTC CCAAGAGTCC TTTGAGGCAG ATGAGCCGGA CAGCAGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGACCTCCA AGCGCTCATC GACATCCCAT GTGCAGAGCT GGCTCAGGAA 
CTCTGCCAAA GCTGTGCCAC TGTCCAGGGG CTGCAGAGCA CACTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCCCGCCA GTCCAAGCAG CTCCTGGAAC TTTACCTCCA GGCCTTGGAG 
AAAGAGGGCA ACATCTTGTC CAACCAGAAA GAGTCCAAAG CTGCCCTCAG TGAAGAGCTG 
GATGCAGTTG ACACAGGCGT CGGCAGAGAG ATGGCTTCGG AAGTGCTGCT CAGAAGCCAG 
ATCCTTACCA CACTGAAGGA GAAGCCTGCC CCAGAGCTGA GTTTATCTAG TCAGGATTTG 
GAGTCTGTCT CCAAGGAGGA TCCCAAAGCC CTGGCTGTCG CTCTGAGCTG GGACATAAGG 
AAGGCAGAGA CAGTCCAGCA GGCCTGCACC ACGGAGCTCG CCCTGCGGCT GCAGCAAGTG 
CAGAGCTTGC ATTCACTCAG GAATCTATCA GCAAGGAGGA GCCCACTGCC TGGGGAACCA 
CAGCGACCCA AACGAGCCAA ACGAGACTCC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020467.1
CDS	110..1105
Translation	MELSRGASAPDPDDVRPLKPCLLRRNHSRDQHGVAASSLEELRSKACELLAIDKSLTPITLVLAEDGTIVDDDDYFLCLPSNTKFVALACNEKWIYNDSDGGTAWVSQESFEADEPDSRAGVKWKNVARQLKEDLSSIILLSEEDLQALIDIPCAELAQELCQSCATVQGLQSTLQQVLDQREEARQSKQLLELYLQALEKEGNILSNQKESKAALSEELDAVDTGVGREMASEVLLRSQILTTLKEKPAPELSLSSQDLESVSKEDPKALAVALSWDIRKAETVQQACTTELALRLQQVQSLHSLRNLSARRSPLPGEPQRPKRAKRDSS

Target ORF information:

RefSeq Version	NM_001025296.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA fragmentation factor, alpha subunit (Dffa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025296.2

ORF Insert Sequence:

ATGGAGCTGT CGCGGGGAGC CAGCGCCCCA GACCCGGACG ATGTCCGGCC TCTCAAACCG 
TGTCTGCTAC GCCGCAACCA CAGCCGCGAT CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTCCTG GCCATTGATA AGTCCCTGAC GCCGATCACC 
CTGGTCCTGG CTGAGGACGG GACCATAGTG GATGACGATG ACTACTTCCT CTGCCTTCCT 
TCCAATACGA AGTTTGTGGC GTTGGCCTGC AATGAGAAGT GGATTTATAA TGATTCCGAT 
GGAGGGACGG CTTGGGTTTC CCAAGAGTCC TTTGAGGCAG ATGAGCCGGA CAGCAGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGACCTCCA AGCGCTCATC GACATCCCAT GTGCAGAGCT GGCTCAGGAA 
CTCTGCCAAA GCTGTGCCAC TGTCCAGGGG CTGCAGAGCA CACTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCCCGCCA GTCCAAGCAG CTCCTGGAAC TTTACCTCCA GGCCTTGGAG 
AAAGAGGGCA ACATCTTGTC CAACCAGAAA GAGTCCAAAG CTGCCCTCAG TGAAGAGCTG 
GATGCAGTTG ACACAGGCGT CGGCAGAGAG ATGGCTTCGG AAGTGCTGCT CAGAAGCCAG 
ATCCTTACCA CACTGAAGGA GAAGCCTGCC CCAGAGCTGA GTTTATCTAG TCAGGATTTG 
GAGTCTGTCT CCAAGGAGGA TCCCAAAGCC CTGGCTGTCG CTCTGAGCTG GGACATAAGG 
AAGGCAGAGA CAGTCCAGCA GGCCTGCACC ACGGAGCTCG CCCTGCGGCT GCAGCAAGTG 
CAGAGCTTGC ATTCACTCAG GAATCTATCA GCAAGGAGGA GCCCACTGCC TGGGGAACCA 
CAGCGACCCA AACGAGCCAA ACGAGACTCC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability.
PloS one8(2)e57871(2013)
Errami Y,Brim H,Oumouna-Benachour K,Oumouna M,Naura AS,Kim H,Ju J,Davis CJ,Kim JG,Ashktorab H,Fallon K,Xu M,Zhang J,Del Valle L,Boulares AH

Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase.
The FEBS journal279(16)2917-28(2012 Aug)
Kutscher D,Pingoud A,Jeltsch A,Meiss G

Engineered apoptotic nucleases for chromatin research.
Nucleic acids research35(13)e93(2007)
Xiao F,Widlak P,Garrard WT

A unique role of the DNA fragmentation factor in maintaining genomic stability.
Proceedings of the National Academy of Sciences of the United States of America103(5)1504-9(2006 Jan)
Yan B,Wang H,Peng Y,Hu Y,Wang H,Zhang X,Chen Q,Bedford JS,Dewhirst MW,Li CY

Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
The Journal of biological chemistry280(7)6005-15(2005 Feb)
Korn C,Scholz SR,Gimadutdinow O,Lurz R,Pingoud A,Meiss G