Ank1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ank1
Entrez Gene ID	11733
Full Name	ankyrin 1, erythroid
Synonyms	Ank-1,nb,pale
General protein information	Preferred Names ankyrin 1, erythroid Names ankyrin-1 erythrocyte ankyrin normoblastic anemia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A2\|8 11.42 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006508991.3	XP_006509054.1	ankyrin-1 isoform X3
XM_006509001.3	XP_006509064.1	ankyrin-1 isoform X13
XM_006509003.3	XP_006509066.1	ankyrin-1 isoform X15
XM_006508989.4	XP_006509052.1	ankyrin-1 isoform X1
XM_006508997.4	XP_006509060.1	ankyrin-1 isoform X9
XM_006508999.4	XP_006509062.1	ankyrin-1 isoform X11
XM_006509000.2	XP_006509063.1	ankyrin-1 isoform X12
NM_001310437.1	NP_001297366.1	ankyrin-1 isoform 9 precursor
NM_001310436.1	NP_001297365.1	ankyrin-1 isoform 8 precursor
XM_006508994.4	XP_006509057.1	ankyrin-1 isoform X6
XM_006508995.4	XP_006509058.1	ankyrin-1 isoform X7
XM_030243251.1	XP_030099111.1	ankyrin-1 isoform X16
XM_006508989.3	XP_006509052.1	ankyrin-1 isoform X1
XM_006508997.3	XP_006509060.1	ankyrin-1 isoform X9
NM_001277281.2	NP_001264210.1	ankyrin-1 isoform 4
NM_031158.4	NP_112435.2	ankyrin-1 isoform 2
XM_006508991.4	XP_006509054.1	ankyrin-1 isoform X3
XM_006508993.4	XP_006509056.1	ankyrin-1 isoform X5
XM_006508996.3	XP_006509059.1	ankyrin-1 isoform X8
XM_006508993.3	XP_006509056.1	ankyrin-1 isoform X5
XM_006508994.3	XP_006509057.1	ankyrin-1 isoform X6
XM_006508995.3	XP_006509058.1	ankyrin-1 isoform X7
XM_006508999.3	XP_006509062.1	ankyrin-1 isoform X11
XM_006509002.3	XP_006509065.1	ankyrin-1 isoform X14
XM_006508992.4	XP_006509055.1	ankyrin-1 isoform X4
XM_006508998.4	XP_006509061.1	ankyrin-1 isoform X10
XM_006509001.4	XP_006509064.1	ankyrin-1 isoform X14
NM_001277286.2	NP_001264215.1	ankyrin-1 isoform 6
NM_001277289.2	NP_001264218.1	ankyrin-1 isoform 7
NM_001110783.3	NP_001104253.1	ankyrin-1 isoform 1
XM_006508992.3	XP_006509055.1	ankyrin-1 isoform X4
XM_006508996.2	XP_006509059.1	ankyrin-1 isoform X8
XM_006508998.3	XP_006509061.1	ankyrin-1 isoform X10
XM_006509004.2	XP_006509067.1	ankyrin-1 isoform X16
NM_001277280.2	NP_001264209.1	ankyrin-1 isoform 3
XM_006508990.2	XP_006509053.1	ankyrin-1 isoform X2
XM_030243250.1	XP_030099110.1	ankyrin-1 isoform X13
XM_006509002.4	XP_006509065.1	ankyrin-1 isoform X15
XM_006509003.4	XP_006509066.1	ankyrin-1 isoform X17
NM_001277284.2	NP_001264213.1	ankyrin-1 isoform 5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANK1	XP_005629869.1
Bos taurus (cattle)	ANK1	XP_002698771.3
Mus musculus (house mouse)	Ank1	NP_001104253.1
Homo sapiens (human)	ANK1	NP_001135918.1
Rattus norvegicus (Norway rat)	Ank1	NP_001100792.1
Pan troglodytes (chimpanzee)	ANK1	XP_003311743.1
	ANK1	XP_001099591.2
Gallus gallus (chicken)	ANK1	XP_424401.4
Danio rerio (zebrafish)	si:ch211-263m18.3	XP_005161295.1

Gene Ontology
Bibliography

Related articles in PubMed

Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria.
Huang HM, Bauer DC, Lelliott PM, Dixon MWA, Tilley L, McMorran BJ, Foote SJ, Burgio G
G3 (Bethesda, Md.)7(9)3133-3144(2017 Sep)

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports7(1)5164(2017 Jul)

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Huang HM, Bauer DC, Lelliott PM, Greth A, McMorran BJ, Foote SJ, Burgio G
Scientific reports637197(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

With increasing age (at 12-15 mo of age) extensor digitorum longus (EDL) skeletal muscles of sAnk1 KO mice develop prematurely large tubular aggregates.
Title: Deletion of small ankyrin 1 (sAnk1) isoforms results in structural and functional alterations in aging skeletal muscle fibers.

Study shows a direct interaction between beta-III spectrin and erythroid ankyrin in the cerebellum and to demonstrate a critical role for beta-III spectrin in maintaining ankyrin R throughout the Purkinje cell dendritic tree.
Title: ?-III spectrin underpins ankyrin R function in Purkinje cell dendritic trees: protein complex critical for sodium channel activity is impaired by SCA5-associated mutations.

Hema6 mutation of ankyrin-1 causes hereditary spherocytosis in mice through a mild reduction of protein expression.
Title: A deep intronic mutation in the ankyrin-1 gene causes diminished protein expression resulting in hemolytic anemia in mice.

Suppression of hepcidin expression and iron overload mediate Salmonella susceptibility in ankyrin 1-mutated mice.
Title: Suppression of hepcidin expression and iron overload mediate Salmonella susceptibility in ankyrin 1 ENU-induced mutant.

We conclude that increased malaria resistance due to ankyrin-1 deficiency is caused by the intraerythrocytic death of P. chabaudi parasites.
Title: A novel ENU-mutation in ankyrin-1 disrupts malaria parasite maturation in red blood cells of mice.

The following Ank1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ank1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67103	NM_001310437.1 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu67102	NM_001310436.1 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu40555	XM_006509004.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu40554	XM_006509003.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89734	XM_030243251.1 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu40540	XM_006508989.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40542	XM_006508991.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40543	XM_006508992.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40544	XM_006508993.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40545	XM_006508994.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40546	XM_006508995.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40547	XM_006508996.3 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40548	XM_006508997.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40549	XM_006508998.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40550	XM_006508999.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40552	XM_006509001.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40553	XM_006509002.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu89733	XM_030243250.1 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu04622	NM_031158.4 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu04682	NM_001110783.3 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1399.30 $1999.00
OMu16865	NM_001277286.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu16933	NM_001277284.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu16966	NM_001277289.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu17251	NM_001277281.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu17276	NM_001277280.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40541	XM_006508990.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40551	XM_006509000.2 Latest version!	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu40540	XM_006508989.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40548	XM_006508997.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40542	XM_006508991.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40544	XM_006508993.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40545	XM_006508994.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40546	XM_006508995.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40550	XM_006508999.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40552	XM_006509001.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40543	XM_006508992.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40547	XM_006508996.2	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40549	XM_006508998.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40553	XM_006509002.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40554	XM_006509003.3	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ank1 ( NM_001310437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297366.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OMu67103
Clone ID Related Accession (Same CDS sequence)	NM_001310437.1
Accession Version	NM_001310437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 9 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U73972.1, AK147483.1 and AI846810.1. On Jul 14, 2015 this sequence version replaced XM_006509006.2. Transcript Variant: This variant (11) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. It encodes isoform 9, which has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U73972.1, BC061219.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTGGACCT TCATCACGCA GCTCCTGGTC ACGCTGGTGC TGTTAGGCTT CTTCCTGGTC 
AGCTGTCAGA ATGTGATGCA TATTGTCAAG GGTTCCCTGT GCTTTGTGCT GAAGCACATC 
CACCAGGAAC TGGACAAGGA GCTGGGGGAG AGCGAGGGCC TGAGCGATGA CGAGGAGACC 
ATCTCTACCA GGGTGGTCCG CCGACGGGTC TTCCTGAAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297366.1
CDS	248..715
Translation	MWTFITQLLVTLVLLGFFLVSCQNVMHIVKGSLCFVLKHIHQELDKELGESEGLSDDEETISTRVVRRRVFLKGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001310437.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310437.1

ORF Insert Sequence:

ATGTGGACCT TCATCACGCA GCTCCTGGTC ACGCTGGTGC TGTTAGGCTT CTTCCTGGTC 
AGCTGTCAGA ATGTGATGCA TATTGTCAAG GGTTCCCTGT GCTTTGTGCT GAAGCACATC 
CACCAGGAAC TGGACAAGGA GCTGGGGGAG AGCGAGGGCC TGAGCGATGA CGAGGAGACC 
ATCTCTACCA GGGTGGTCCG CCGACGGGTC TTCCTGAAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001310436.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297365.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OMu67102
Clone ID Related Accession (Same CDS sequence)	NM_001310436.1
Accession Version	NM_001310436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U73972.1, AK134267.1, BC079910.1, AK147483.1 and AI846810.1. On Jul 14, 2015 this sequence version replaced XM_006509005.2. Transcript Variant: This variant (10) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR, translation initiation at an alternate start codon, and a novel 3' coding region. The encoded isoform (8) is shorter than and has distinct N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK134267.1, CJ049270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTGGACCT TCATCACGCA GCTCCTGGTC ACGCTGGTGC TGTTAGGCTT CTTCCTGGTC 
AGCTGTCAGA ATGTGATGCA TATTGTCAAG GGTTCCCTGT GCTTTGTGCT GAAGCACATC 
CACCAGGAAC TGGACAAGGA GCTGGGGGAG AGCGAGGGCC TGAGCGATGA CGAGGAGACC 
ATCTCTACCA GGGTGGTCCG CCGACGGGTC TTCCTGAAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGATCAC ACCTCAACAC CCAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297365.1
CDS	248..718
Translation	MWTFITQLLVTLVLLGFFLVSCQNVMHIVKGSLCFVLKHIHQELDKELGESEGLSDDEETISTRVVRRRVFLKGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	NM_001310436.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310436.1

ORF Insert Sequence:

ATGTGGACCT TCATCACGCA GCTCCTGGTC ACGCTGGTGC TGTTAGGCTT CTTCCTGGTC 
AGCTGTCAGA ATGTGATGCA TATTGTCAAG GGTTCCCTGT GCTTTGTGCT GAAGCACATC 
CACCAGGAAC TGGACAAGGA GCTGGGGGAG AGCGAGGGCC TGAGCGATGA CGAGGAGACC 
ATCTCTACCA GGGTGGTCCG CCGACGGGTC TTCCTGAAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGATCAC ACCTCAACAC CCAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509004.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509067.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu40555
Clone ID Related Accession (Same CDS sequence)	XM_006509004.2
Accession Version	XM_006509004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACCT TCATCACGCA GCTCCTGGTC ACGCTGGTGC TGTTAGGCTT CTTCCTGGTC 
AGCTGTCAGA ATGTGATGCA TATTGTCAAG GGTTCCCTGT GCTTTGTGCT GAAGCACATC 
CACCAGGAAC TGGACAAGGA GCTGGGGGAG AGCGAGGGCC TGAGCGATGA CGAGGAGACC 
ATCTCTACCA GGGTGGTCCG CCGACGGGTC TTCCTGAAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGTGGAG CTAAGAGGGA GTGGACTCCA GCCGGACCTG 
ATAGAGGGCA GGAAGGGGGC TCAGATAGTG AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509067.1
CDS	3437..3979
Translation	MWTFITQLLVTLVLLGFFLVSCQNVMHIVKGSLCFVLKHIHQELDKELGESEGLSDDEETISTRVVRRRVFLKGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006509004.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509004.2

ORF Insert Sequence:

ATGTGGACCT TCATCACGCA GCTCCTGGTC ACGCTGGTGC TGTTAGGCTT CTTCCTGGTC 
AGCTGTCAGA ATGTGATGCA TATTGTCAAG GGTTCCCTGT GCTTTGTGCT GAAGCACATC 
CACCAGGAAC TGGACAAGGA GCTGGGGGAG AGCGAGGGCC TGAGCGATGA CGAGGAGACC 
ATCTCTACCA GGGTGGTCCG CCGACGGGTC TTCCTGAAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGTGGAG CTAAGAGGGA GTGGACTCCA GCCGGACCTG 
ATAGAGGGCA GGAAGGGGGC TCAGATAGTG AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509003.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509066.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X18, mRNA.

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CloneID	OMu40554
Clone ID Related Accession (Same CDS sequence)	XM_006509003.3 , XM_006509003.4
Accession Version	XM_006509003.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509003.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGCTTGTGGT 
TGCAGAGCTG GGGAGCCTGG AGGAGCTGGA GAAGAAACGT GTGTGCCGCA TCATCACCAC 
CGACTTCCCT CTGTACTTTG TCATCATGTC CCGGCTCTGC CAGGACTACG ACACCATCGG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509066.1
CDS	567..3992
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEACGCRAGEPGGAGEETCVPHHHHRLPSVLCHHVPALPGLRHHRS

Target ORF information:

RefSeq Version	XM_006509003.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509003.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGCTTGTGGT 
TGCAGAGCTG GGGAGCCTGG AGGAGCTGGA GAAGAAACGT GTGTGCCGCA TCATCACCAC 
CGACTTCCCT CTGTACTTTG TCATCATGTC CCGGCTCTGC CAGGACTACG ACACCATCGG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_030243251.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099111.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X17, mRNA.

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CloneID	OMu89734
Clone ID Related Accession (Same CDS sequence)	XM_030243251.1
Accession Version	XM_030243251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGTGGAG 
CTAAGAGGGA GTGGACTCCA GCCGGACCTG ATAGAGGGCA GGAAGGGGGC TCAGATAGTG 
AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099111.1
CDS	738..4490
Translation	MVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_030243251.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243251.1

ORF Insert Sequence:

ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGTGGAG 
CTAAGAGGGA GTGGACTCCA GCCGGACCTG ATAGAGGGCA GGAAGGGGGC TCAGATAGTG 
AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509003.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509066.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X16, mRNA.

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CloneID	OMu40554
Clone ID Related Accession (Same CDS sequence)	XM_006509003.3 , XM_006509003.4
Accession Version	XM_006509003.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGCTTGTGGT 
TGCAGAGCTG GGGAGCCTGG AGGAGCTGGA GAAGAAACGT GTGTGCCGCA TCATCACCAC 
CGACTTCCCT CTGTACTTTG TCATCATGTC CCGGCTCTGC CAGGACTACG ACACCATCGG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509066.1
CDS	350..3775
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEACGCRAGEPGGAGEETCVPHHHHRLPSVLCHHVPALPGLRHHRS

Target ORF information:

RefSeq Version	XM_006509003.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509003.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGCTTGTGGT 
TGCAGAGCTG GGGAGCCTGG AGGAGCTGGA GAAGAAACGT GTGTGCCGCA TCATCACCAC 
CGACTTCCCT CTGTACTTTG TCATCATGTC CCGGCTCTGC CAGGACTACG ACACCATCGG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508989.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509052.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40540
Clone ID Related Accession (Same CDS sequence)	XM_006508989.4 , XM_006508989.3
Accession Version	XM_006508989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508989.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGTGGAG 
CTAAGAGGGA GTGGACTCCA GCCGGACCTG ATAGAGGGCA GGAAGGGGGC TCAGATAGTG 
AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509052.1
CDS	2828..8680
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508989.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508989.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGTGGAG 
CTAAGAGGGA GTGGACTCCA GCCGGACCTG ATAGAGGGCA GGAAGGGGGC TCAGATAGTG 
AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508991.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509054.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X3, mRNA.

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CloneID	OMu40542
Clone ID Related Accession (Same CDS sequence)	XM_006508991.3 , XM_006508991.4
Accession Version	XM_006508991.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508991.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGATCAC 
ACCTCAACAC CCAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509054.1
CDS	567..6347
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508991.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508991.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGATCAC 
ACCTCAACAC CCAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508992.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509055.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X4, mRNA.

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CloneID	OMu40543
Clone ID Related Accession (Same CDS sequence)	XM_006508992.4 , XM_006508992.3
Accession Version	XM_006508992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508992.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGGAGCTAAG AGGGAGTGGA 
CTCCAGCCGG ACCTGATAGA GGGCAGGAAG GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG 
AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509055.1
CDS	567..6344
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508992.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508992.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGGAGCTAAG AGGGAGTGGA 
CTCCAGCCGG ACCTGATAGA GGGCAGGAAG GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG 
AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508993.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509056.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X5, mRNA.

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CloneID	OMu40544
Clone ID Related Accession (Same CDS sequence)	XM_006508993.4 , XM_006508993.3
Accession Version	XM_006508993.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGCCAGCAGC GAGTTCACGC CCGAATCACA GACTCACCCT CAGTGAGGCA GGTGCTGGAC 
AGAAGCCAGG CCAGAACACT GGACTGGGAT AAACAGGGTT CCACAGCGGT ACACCCGCAA 
GAAGCCACAC AGAGCTCCTG GCAAGAGGAG GTCACGCAGG GCCCACACTC ATTCCAGAGA 
AGGATCACCA CCATCCAAGG GCCGGAGCCT GGTGCGCTTC AGGAATACGA GCAGGTGCTG 
GTGTCTACCA GGGAGCATGT GCAGAGGGGG CCACCTGAGA CCGGCAGCCC CAAAGCTGGC 
AAGGAACCTA GCCTGTGGGC ACCTGAGAGC GCCTTCTCTC AAGAGGTGCA GGGGGATGAG 
CTTCAGAATA TTCCAGGAGA GCAGGTGACG GAGGAACAAT TCACAGATGA ACAGGGCAAC 
ATTGTTACCA AGAAGATCAT TCGCAAAGTC GTCCGGCAGG TAGACTCGTC TGGTGCCATC 
GACACCCAGC AGCACGAGGA GGTGATTGTC GAGGGTCCCC TGGCAGACCC TGGGGATCTG 
GAAGCAGATA TTGAGTCCTT TATGAAACTT ACTAAGGTGG AGCTAAGAGG GAGTGGACTC 
CAGCCGGACC TGATAGAGGG CAGGAAGGGG GCTCAGATAG TGAAGCGGGC CAGCCTGAAA 
AGGGGCAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509056.1
CDS	567..6341
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508993.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508993.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGCCAGCAGC GAGTTCACGC CCGAATCACA GACTCACCCT CAGTGAGGCA GGTGCTGGAC 
AGAAGCCAGG CCAGAACACT GGACTGGGAT AAACAGGGTT CCACAGCGGT ACACCCGCAA 
GAAGCCACAC AGAGCTCCTG GCAAGAGGAG GTCACGCAGG GCCCACACTC ATTCCAGAGA 
AGGATCACCA CCATCCAAGG GCCGGAGCCT GGTGCGCTTC AGGAATACGA GCAGGTGCTG 
GTGTCTACCA GGGAGCATGT GCAGAGGGGG CCACCTGAGA CCGGCAGCCC CAAAGCTGGC 
AAGGAACCTA GCCTGTGGGC ACCTGAGAGC GCCTTCTCTC AAGAGGTGCA GGGGGATGAG 
CTTCAGAATA TTCCAGGAGA GCAGGTGACG GAGGAACAAT TCACAGATGA ACAGGGCAAC 
ATTGTTACCA AGAAGATCAT TCGCAAAGTC GTCCGGCAGG TAGACTCGTC TGGTGCCATC 
GACACCCAGC AGCACGAGGA GGTGATTGTC GAGGGTCCCC TGGCAGACCC TGGGGATCTG 
GAAGCAGATA TTGAGTCCTT TATGAAACTT ACTAAGGTGG AGCTAAGAGG GAGTGGACTC 
CAGCCGGACC TGATAGAGGG CAGGAAGGGG GCTCAGATAG TGAAGCGGGC CAGCCTGAAA 
AGGGGCAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508994.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509057.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X6, mRNA.

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CloneID	OMu40545
Clone ID Related Accession (Same CDS sequence)	XM_006508994.4 , XM_006508994.3
Accession Version	XM_006508994.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508994.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGTGGAG CTAAGAGGGA GTGGACTCCA GCCGGACCTG 
ATAGAGGGCA GGAAGGGGGC TCAGATAGTG AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509057.1
CDS	567..6329
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508994.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508994.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGTGGAG CTAAGAGGGA GTGGACTCCA GCCGGACCTG 
ATAGAGGGCA GGAAGGGGGC TCAGATAGTG AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508995.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509058.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X7, mRNA.

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CloneID	OMu40546
Clone ID Related Accession (Same CDS sequence)	XM_006508995.4 , XM_006508995.3
Accession Version	XM_006508995.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508995.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGTGAT TGTCGAGGGT CCCCTGGCAG ACCCTGGGGA TCTGGAAGCA 
GATATTGAGT CCTTTATGAA ACTTACTAAG GATCACACCT CAACACCCAA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509058.1
CDS	567..6323
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508995.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508995.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGTGAT TGTCGAGGGT CCCCTGGCAG ACCCTGGGGA TCTGGAAGCA 
GATATTGAGT CCTTTATGAA ACTTACTAAG GATCACACCT CAACACCCAA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508996.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509059.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X8, mRNA.

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CloneID	OMu40547
Clone ID Related Accession (Same CDS sequence)	XM_006508996.3 , XM_006508996.2
Accession Version	XM_006508996.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GTCAGAGGTC AGAAAAGAAG 
AGGCAGGAAG TCTCAGGCAC AGAGCAGGAC ACGGAGACTG AAGTGTCTCT TGTTTCAGGC 
CAGCAGCGAG TTCACGCCCG AATCACAGAC TCACCCTCAG TGAGGCAGGT GCTGGACAGA 
AGCCAGGCCA GAACACTGGA CTGGGATAAA CAGGGTTCCA CAGCGGTACA CCCGCAAGAA 
GCCACACAGA GCTCCTGGCA AGAGGAGGTC ACGCAGGGCC CACACTCATT CCAGAGAAGG 
ATCACCACCA TCCAAGGGCC GGAGCCTGGT GCGCTTCAGG AATACGAGCA GGTGCTGGTG 
TCTACCAGGG AGCATGTGCA GAGGGGGCCA CCTGAGACCG GCAGCCCCAA AGCTGGCAAG 
GAACCTAGCC TGTGGGCACC TGAGAGCGCC TTCTCTCAAG AGGTGCAGGG GGATGAGCTT 
CAGAATATTC CAGGAGAGCA GGTGACGGAG GAACAATTCA CAGATGAACA GGGCAACATT 
GTTACCAAGA AGATCATTCG CAAAGTCGTC CGGCAGGTAG ACTCGTCTGG TGCCATCGAC 
ACCCAGCAGC ACGAGGAGGT GATTGTCGAG GGTCCCCTGG CAGACCCTGG GGATCTGGAA 
GCAGATATTG AGTCCTTTAT GAAACTTACT AAGGTGGAGC TAAGAGGGAG TGGACTCCAG 
CCGGACCTGA TAGAGGGCAG GAAGGGGGCT CAGATAGTGA AGCGGGCCAG CCTGAAAAGG 
GGCAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509059.1
CDS	365..6076
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508996.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508996.3

ORF Insert Sequence:

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GTCAGAGGTC AGAAAAGAAG 
AGGCAGGAAG TCTCAGGCAC AGAGCAGGAC ACGGAGACTG AAGTGTCTCT TGTTTCAGGC 
CAGCAGCGAG TTCACGCCCG AATCACAGAC TCACCCTCAG TGAGGCAGGT GCTGGACAGA 
AGCCAGGCCA GAACACTGGA CTGGGATAAA CAGGGTTCCA CAGCGGTACA CCCGCAAGAA 
GCCACACAGA GCTCCTGGCA AGAGGAGGTC ACGCAGGGCC CACACTCATT CCAGAGAAGG 
ATCACCACCA TCCAAGGGCC GGAGCCTGGT GCGCTTCAGG AATACGAGCA GGTGCTGGTG 
TCTACCAGGG AGCATGTGCA GAGGGGGCCA CCTGAGACCG GCAGCCCCAA AGCTGGCAAG 
GAACCTAGCC TGTGGGCACC TGAGAGCGCC TTCTCTCAAG AGGTGCAGGG GGATGAGCTT 
CAGAATATTC CAGGAGAGCA GGTGACGGAG GAACAATTCA CAGATGAACA GGGCAACATT 
GTTACCAAGA AGATCATTCG CAAAGTCGTC CGGCAGGTAG ACTCGTCTGG TGCCATCGAC 
ACCCAGCAGC ACGAGGAGGT GATTGTCGAG GGTCCCCTGG CAGACCCTGG GGATCTGGAA 
GCAGATATTG AGTCCTTTAT GAAACTTACT AAGGTGGAGC TAAGAGGGAG TGGACTCCAG 
CCGGACCTGA TAGAGGGCAG GAAGGGGGCT CAGATAGTGA AGCGGGCCAG CCTGAAAAGG 
GGCAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508997.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509060.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X9, mRNA.

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CloneID	OMu40548
Clone ID Related Accession (Same CDS sequence)	XM_006508997.4 , XM_006508997.3
Accession Version	XM_006508997.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508997.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG ATCACACCTC AACACCCAAA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509060.1
CDS	567..6272
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508997.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508997.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG ATCACACCTC AACACCCAAA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508998.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509061.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X10, mRNA.

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CloneID	OMu40549
Clone ID Related Accession (Same CDS sequence)	XM_006508998.4 , XM_006508998.3
Accession Version	XM_006508998.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508998.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509061.1
CDS	567..6254
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508998.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508998.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508999.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509062.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X11, mRNA.

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CloneID	OMu40550
Clone ID Related Accession (Same CDS sequence)	XM_006508999.4 , XM_006508999.3
Accession Version	XM_006508999.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508999.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGATCA CACCTCAACA CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509062.1
CDS	567..6248
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508999.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508999.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGATCA CACCTCAACA CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509001.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509064.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X14, mRNA.

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CloneID	OMu40552
Clone ID Related Accession (Same CDS sequence)	XM_006509001.3 , XM_006509001.4
Accession Version	XM_006509001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509001.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGCCAGC AGCGAGTTCA CGCCCGAATC 
ACAGACTCAC CCTCAGTGAG GCAGGTGCTG GACAGAAGCC AGGCCAGAAC ACTGGACTGG 
GATAAACAGG GTTCCACAGC GGTACACCCG CAAGAAGCCA CACAGAGCTC CTGGCAAGAG 
GAGGTCACGC AGGGCCCACA CTCATTCCAG AGAAGGATCA CCACCATCCA AGGGCCGGAG 
CCTGGTGCGC TTCAGGAATA CGAGCAGGTG CTGGTGTCTA CCAGGGAGCA TGTGCAGAGG 
GGGCCACCTG AGACCGGCAG CCCCAAAGCT GGCAAGGAAC CTAGCCTGTG GGCACCTGAG 
AGCGCCTTCT CTCAAGAGGT GCAGGGGGAT GAGCTTCAGA ATATTCCAGG AGAGCAGGTG 
ACGGAGGAAC AATTCACAGA TGAACAGGGC AACATTGTTA CCAAGAAGAT CATTCGCAAA 
GTCGTCCGGC AGGTAGACTC GTCTGGTGCC ATCGACACCC AGCAGCACGA GGAGGTGATT 
GTCGAGGGTC CCCTGGCAGA CCCTGGGGAT CTGGAAGCAG ATATTGAGTC CTTTATGAAA 
CTTACTAAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509064.1
CDS	567..5954
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006509001.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509001.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGCCAGC AGCGAGTTCA CGCCCGAATC 
ACAGACTCAC CCTCAGTGAG GCAGGTGCTG GACAGAAGCC AGGCCAGAAC ACTGGACTGG 
GATAAACAGG GTTCCACAGC GGTACACCCG CAAGAAGCCA CACAGAGCTC CTGGCAAGAG 
GAGGTCACGC AGGGCCCACA CTCATTCCAG AGAAGGATCA CCACCATCCA AGGGCCGGAG 
CCTGGTGCGC TTCAGGAATA CGAGCAGGTG CTGGTGTCTA CCAGGGAGCA TGTGCAGAGG 
GGGCCACCTG AGACCGGCAG CCCCAAAGCT GGCAAGGAAC CTAGCCTGTG GGCACCTGAG 
AGCGCCTTCT CTCAAGAGGT GCAGGGGGAT GAGCTTCAGA ATATTCCAGG AGAGCAGGTG 
ACGGAGGAAC AATTCACAGA TGAACAGGGC AACATTGTTA CCAAGAAGAT CATTCGCAAA 
GTCGTCCGGC AGGTAGACTC GTCTGGTGCC ATCGACACCC AGCAGCACGA GGAGGTGATT 
GTCGAGGGTC CCCTGGCAGA CCCTGGGGAT CTGGAAGCAG ATATTGAGTC CTTTATGAAA 
CTTACTAAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509002.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509065.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X15, mRNA.

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CloneID	OMu40553
Clone ID Related Accession (Same CDS sequence)	XM_006509002.3 , XM_006509002.4
Accession Version	XM_006509002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509002.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGATTGTCG AGGGTCCCCT GGCAGACCCT 
GGGGATCTGG AAGCAGATAT TGAGTCCTTT ATGAAACTTA CTAAGGATCA CACCTCAACA 
CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509065.1
CDS	567..5858
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	XM_006509002.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509002.4

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGATTGTCG AGGGTCCCCT GGCAGACCCT 
GGGGATCTGG AAGCAGATAT TGAGTCCTTT ATGAAACTTA CTAAGGATCA CACCTCAACA 
CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_030243250.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099110.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X13, mRNA.

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CloneID	OMu89733
Clone ID Related Accession (Same CDS sequence)	XM_030243250.1
Accession Version	XM_030243250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGCCAGCAGC GAGTTCACGC CCGAATCACA GACTCACCCT CAGTGAGGCA GGTGCTGGAC 
AGAAGCCAGG CCAGAACACT GGACTGGGAT AAACAGGGTT CCACAGCGGT ACACCCGCAA 
GAAGCCACAC AGAGCTCCTG GCAAGAGGAG GTCACGCAGG GCCCACACTC ATTCCAGAGA 
AGGATCACCA CCATCCAAGG GCCGGAGCCT GGTGCGCTTC AGGAATACGA GCAGGTGCTG 
GTGTCTACCA GGGAGCATGT GCAGAGGGGG CCACCTGAGA CCGGCAGCCC CAAAGCTGGC 
AAGGAACCTA GCCTGTGGGC ACCTGAGAGC GCCTTCTCTC AAGAGGTGCA GGGGGATGAG 
CTTCAGAATA TTCCAGGAGA GCAGGTGACG GAGGAACAAT TCACAGATGA ACAGGGCAAC 
ATTGTTACCA AGAAGATCAT TCGCAAAGTC GTCCGGCAGG TAGACTCGTC TGGTGCCATC 
GACACCCAGC AGCACGAGGA GGATCACACC TCAACACCCA AACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099110.1
CDS	567..6194
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEDHTSTPKP

Target ORF information:

RefSeq Version	XM_030243250.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243250.1

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGCCAGCAGC GAGTTCACGC CCGAATCACA GACTCACCCT CAGTGAGGCA GGTGCTGGAC 
AGAAGCCAGG CCAGAACACT GGACTGGGAT AAACAGGGTT CCACAGCGGT ACACCCGCAA 
GAAGCCACAC AGAGCTCCTG GCAAGAGGAG GTCACGCAGG GCCCACACTC ATTCCAGAGA 
AGGATCACCA CCATCCAAGG GCCGGAGCCT GGTGCGCTTC AGGAATACGA GCAGGTGCTG 
GTGTCTACCA GGGAGCATGT GCAGAGGGGG CCACCTGAGA CCGGCAGCCC CAAAGCTGGC 
AAGGAACCTA GCCTGTGGGC ACCTGAGAGC GCCTTCTCTC AAGAGGTGCA GGGGGATGAG 
CTTCAGAATA TTCCAGGAGA GCAGGTGACG GAGGAACAAT TCACAGATGA ACAGGGCAAC 
ATTGTTACCA AGAAGATCAT TCGCAAAGTC GTCCGGCAGG TAGACTCGTC TGGTGCCATC 
GACACCCAGC AGCACGAGGA GGATCACACC TCAACACCCA AACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_031158.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112435.2 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 2, mRNA.

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CloneID	OMu04622
Clone ID Related Accession (Same CDS sequence)	NM_031158.4
Accession Version	NM_031158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147483.1, X69063.1, AK147278.1 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_031158.3. Transcript Variant: This variant (2) differs in the 5' UTR, represents use of an alternate promoter, initiates translation at an alternate start codon, and lacks an alternate in-frame segment compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X69063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGC CAGCAGCGAG TTCACGCCCG AATCACAGAC 
TCACCCTCAG TGAGGCAGGT GCTGGACAGA AGCCAGGCCA GAACACTGGA CTGGGATAAA 
CAGGGTTCCA CAGCGGTACA CCCGCAAGAA GCCACACAGA GCTCCTGGCA AGAGGAGGTC 
ACGCAGGGCC CACACTCATT CCAGAGAAGG ATCACCACCA TCCAAGGGCC GGAGCCTGGT 
GCGCTTCAGG AATACGAGCA GGTGCTGGTG TCTACCAGGG AGCATGTGCA GAGGGGGCCA 
CCTGAGACCG GCAGCCCCAA AGCTGGCAAG GAACCTAGCC TGTGGGCACC TGAGAGCGCC 
TTCTCTCAAG AGGTGCAGGG GGATGAGCTT CAGAATATTC CAGGAGAGCA GGTGACGGAG 
GAACAATTCA CAGATGAACA GGGCAACATT GTTACCAAGA AGATCATTCG CAAAGTCGTC 
CGGCAGGTAG ACTCGTCTGG TGCCATCGAC ACCCAGCAGC ACGAGGAGGT GGAGCTAAGA 
GGGAGTGGAC TCCAGCCGGA CCTGATAGAG GGCAGGAAGG GGGCTCAGAT AGTGAAGCGG 
GCCAGCCTGA AAAGGGGCAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112435.2
CDS	374..5920
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_031158.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031158.4

ORF Insert Sequence:

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGC CAGCAGCGAG TTCACGCCCG AATCACAGAC 
TCACCCTCAG TGAGGCAGGT GCTGGACAGA AGCCAGGCCA GAACACTGGA CTGGGATAAA 
CAGGGTTCCA CAGCGGTACA CCCGCAAGAA GCCACACAGA GCTCCTGGCA AGAGGAGGTC 
ACGCAGGGCC CACACTCATT CCAGAGAAGG ATCACCACCA TCCAAGGGCC GGAGCCTGGT 
GCGCTTCAGG AATACGAGCA GGTGCTGGTG TCTACCAGGG AGCATGTGCA GAGGGGGCCA 
CCTGAGACCG GCAGCCCCAA AGCTGGCAAG GAACCTAGCC TGTGGGCACC TGAGAGCGCC 
TTCTCTCAAG AGGTGCAGGG GGATGAGCTT CAGAATATTC CAGGAGAGCA GGTGACGGAG 
GAACAATTCA CAGATGAACA GGGCAACATT GTTACCAAGA AGATCATTCG CAAAGTCGTC 
CGGCAGGTAG ACTCGTCTGG TGCCATCGAC ACCCAGCAGC ACGAGGAGGT GGAGCTAAGA 
GGGAGTGGAC TCCAGCCGGA CCTGATAGAG GGCAGGAAGG GGGCTCAGAT AGTGAAGCGG 
GCCAGCCTGA AAAGGGGCAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001110783.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001104253.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 1, mRNA.

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CloneID	OMu04682
Clone ID Related Accession (Same CDS sequence)	NM_001110783.3
Accession Version	NM_001110783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ105945.1, AK158131.1, AK147278.1, AK147483.1 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_001110783.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GAACTGCTCA CATATCTATA 
ATGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAGTC TCCAGCCATC 
CCAAGGATCC CTTGTGTCAC ACCTGAGACC GTTGTGATCC GATCTGAAGA TCAGGAACAA 
GCATCTAAAG AATATGATGA GGATTCCCTC ATTCCCAGCA GCCCGGCCAC AGAGACCTCA 
GACAACATTA GCCCTGTGGC TAGCCCGGTC CACACAGGGT TCCTGGTGAG CTTCATGGTG 
GATGCCCGAG GGGGATCCAT GAGAGGAAGC CGTCATAATG GCCTCAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG 
AACACGCCAC CCCCACTGGC TGAGGAAGAG GGCCTAGCCA GCAGGATCAT TGCCCTGGGA 
CCCACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCATTT TGCCTCCCAT 
GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG 
CACAAGAGTC GTTATGGGGA AAGCTACCTG GACCAGATCC TTAATGGCAT GGATGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA CGTGTGTGCC GCATCATCAC CACCGACTTC 
CCTCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGTTCCC TGAGAAGCAA GCTGGTACCG CTGGTACAGG CAACGTTCCC TGAAAACGCT 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCGGATGA GCTGGTGACC 
AAGCTCCTGG GTAACCAGGC CACATTCAGC CCCATTGTGA CCGTGGAGCC GAGGCGTCGG 
AAGTTCCACC GTCCCATCGG GCTGCGAATC CCGCTCCCTC CCTCATGGAC GGACAACCCC 
AGGGACAGTG GGGAGGGAGA CACCACCAGC CTGCGCCTAC TGTGTAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AACTTATATA CGCCAATGAG 
TGTGCCAACT TTACCACCAA TGTCTCAGCC AGGTTCTGGC TGTCAGACTG TCCCCGGACT 
GCGGAGGCTG TGCACTTTGC CACTCTCCTG TACAAAGAGC TCACAGCAGT CCCCTACATG 
GCTAAGTTTG TCATTTTTGC CAAGATGAAC GACGCTCGGG AAGGACGGCT CCGTTGCTAC 
TGCATGACGG ATGACAAAGT GGACAAGACC CTGGAGCAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTGGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTTC CTGTCAAGAA AGCGGCCCAA CAGCGGAGCT TCCATTTCCA GTCGTTTCGA 
GAGAATCGTC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TACGAAAGAC AATGAAGTAC GAGGACACAC AGCACATCCT CTGTCACCTG 
AATATCACCA TGCCCCCCTG CACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACACCCCTGA CCCTTCGATA CAGCATTCTC AGCGAGTCGC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TCGAAATGAG GATGGCTGTC ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA 
GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG GAAGACATCA ACCGGATCCG TGTAGAAAAC 
CCTAACTCCT TGTTGGACCA GAGTACAGCC TTGCTGACCC TCTGGGTGGA CCGTGAAGGC 
GAAAATGCAA AGATGGAGAA TTTGTACACA GCCCTGCGGA ACATCGACAG GAGTGAGATT 
GTTAACATGT TGGAGGGCTC CGGCAGGCAG AGCAGAAACC TCAAACCAGA GCGGAGACAT 
GGGGACCGGG AGTACTCATT GTCACCCTCC CAGGTGAATG GTTACTCCTC GCTGCAGGAC 
GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC GCTCTCCCCT CTCCACTGTG TGCAGACCAG 
TACTGGAACG AAGTGGCCGT CATAGACGCC ATCCCCCTGG CGGCTACAGA GCATGACACC 
ATGCTGGAGA TGTCTGACAT GCAGGTGTGG TCTGCGGGCC TCACACCCTC CCTGGTCACT 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CTGACGCCAT ACCAGAGTGG 
AAGTTGGAAG GGGCACACTC AGAGGACACG CAGGGCCCGG AGCTGGGCTC TCAGGACCTG 
GTGGAGGACG ACACAGTGGA TTCAGATGCC ACAAATGGCC TGGCAGATTT GCTAGGTCAG 
GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG CAGGAAGTCT CAGGCACAGA GCAGGACACG 
GAGACTGAAG TGTCTCTTGT TTCAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CACTGGACTG GGATAAACAG 
GGTTCCACAG CGGTACACCC GCAAGAAGCC ACACAGAGCT CCTGGCAAGA GGAGGTCACG 
CAGGGCCCAC ACTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCTGGTGCG 
CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGACCGGCA GCCCCAAAGC TGGCAAGGAA CCTAGCCTGT GGGCACCTGA GAGCGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACGGAGGAA 
CAATTCACAG ATGAACAGGG CAACATTGTT ACCAAGAAGA TCATTCGCAA AGTCGTCCGG 
CAGGTAGACT CGTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGTGGA GCTAAGAGGG 
AGTGGACTCC AGCCGGACCT GATAGAGGGC AGGAAGGGGG CTCAGATAGT GAAGCGGGCC 
AGCCTGAAAA GGGGCAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104253.1
CDS	301..6024
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001110783.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110783.3

ORF Insert Sequence:

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GAACTGCTCA CATATCTATA 
ATGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAGTC TCCAGCCATC 
CCAAGGATCC CTTGTGTCAC ACCTGAGACC GTTGTGATCC GATCTGAAGA TCAGGAACAA 
GCATCTAAAG AATATGATGA GGATTCCCTC ATTCCCAGCA GCCCGGCCAC AGAGACCTCA 
GACAACATTA GCCCTGTGGC TAGCCCGGTC CACACAGGGT TCCTGGTGAG CTTCATGGTG 
GATGCCCGAG GGGGATCCAT GAGAGGAAGC CGTCATAATG GCCTCAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG 
AACACGCCAC CCCCACTGGC TGAGGAAGAG GGCCTAGCCA GCAGGATCAT TGCCCTGGGA 
CCCACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCATTT TGCCTCCCAT 
GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG 
CACAAGAGTC GTTATGGGGA AAGCTACCTG GACCAGATCC TTAATGGCAT GGATGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA CGTGTGTGCC GCATCATCAC CACCGACTTC 
CCTCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGTTCCC TGAGAAGCAA GCTGGTACCG CTGGTACAGG CAACGTTCCC TGAAAACGCT 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCGGATGA GCTGGTGACC 
AAGCTCCTGG GTAACCAGGC CACATTCAGC CCCATTGTGA CCGTGGAGCC GAGGCGTCGG 
AAGTTCCACC GTCCCATCGG GCTGCGAATC CCGCTCCCTC CCTCATGGAC GGACAACCCC 
AGGGACAGTG GGGAGGGAGA CACCACCAGC CTGCGCCTAC TGTGTAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AACTTATATA CGCCAATGAG 
TGTGCCAACT TTACCACCAA TGTCTCAGCC AGGTTCTGGC TGTCAGACTG TCCCCGGACT 
GCGGAGGCTG TGCACTTTGC CACTCTCCTG TACAAAGAGC TCACAGCAGT CCCCTACATG 
GCTAAGTTTG TCATTTTTGC CAAGATGAAC GACGCTCGGG AAGGACGGCT CCGTTGCTAC 
TGCATGACGG ATGACAAAGT GGACAAGACC CTGGAGCAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTGGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTTC CTGTCAAGAA AGCGGCCCAA CAGCGGAGCT TCCATTTCCA GTCGTTTCGA 
GAGAATCGTC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TACGAAAGAC AATGAAGTAC GAGGACACAC AGCACATCCT CTGTCACCTG 
AATATCACCA TGCCCCCCTG CACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACACCCCTGA CCCTTCGATA CAGCATTCTC AGCGAGTCGC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TCGAAATGAG GATGGCTGTC ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA 
GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG GAAGACATCA ACCGGATCCG TGTAGAAAAC 
CCTAACTCCT TGTTGGACCA GAGTACAGCC TTGCTGACCC TCTGGGTGGA CCGTGAAGGC 
GAAAATGCAA AGATGGAGAA TTTGTACACA GCCCTGCGGA ACATCGACAG GAGTGAGATT 
GTTAACATGT TGGAGGGCTC CGGCAGGCAG AGCAGAAACC TCAAACCAGA GCGGAGACAT 
GGGGACCGGG AGTACTCATT GTCACCCTCC CAGGTGAATG GTTACTCCTC GCTGCAGGAC 
GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC GCTCTCCCCT CTCCACTGTG TGCAGACCAG 
TACTGGAACG AAGTGGCCGT CATAGACGCC ATCCCCCTGG CGGCTACAGA GCATGACACC 
ATGCTGGAGA TGTCTGACAT GCAGGTGTGG TCTGCGGGCC TCACACCCTC CCTGGTCACT 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CTGACGCCAT ACCAGAGTGG 
AAGTTGGAAG GGGCACACTC AGAGGACACG CAGGGCCCGG AGCTGGGCTC TCAGGACCTG 
GTGGAGGACG ACACAGTGGA TTCAGATGCC ACAAATGGCC TGGCAGATTT GCTAGGTCAG 
GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG CAGGAAGTCT CAGGCACAGA GCAGGACACG 
GAGACTGAAG TGTCTCTTGT TTCAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CACTGGACTG GGATAAACAG 
GGTTCCACAG CGGTACACCC GCAAGAAGCC ACACAGAGCT CCTGGCAAGA GGAGGTCACG 
CAGGGCCCAC ACTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCTGGTGCG 
CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGACCGGCA GCCCCAAAGC TGGCAAGGAA CCTAGCCTGT GGGCACCTGA GAGCGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACGGAGGAA 
CAATTCACAG ATGAACAGGG CAACATTGTT ACCAAGAAGA TCATTCGCAA AGTCGTCCGG 
CAGGTAGACT CGTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGTGGA GCTAAGAGGG 
AGTGGACTCC AGCCGGACCT GATAGAGGGC AGGAAGGGGG CTCAGATAGT GAAGCGGGCC 
AGCCTGAAAA GGGGCAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001277286.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264215.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 6, mRNA.

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CloneID	OMu16865
Clone ID Related Accession (Same CDS sequence)	NM_001277286.2
Accession Version	NM_001277286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147483.1, X69063.1, AK147597.1 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_001277286.1. Transcript Variant: This variant (6) differs in the 5' UTR, represents use of an alternate promoter, initiates translation at an alternate start codon, and lacks an alternate in-frame segment compared to variant 1. The encoded isoform (6) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138029.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GCCAGCAGCG AGTTCACGCC 
CGAATCACAG ACTCACCCTC AGTGAGGCAG GTGCTGGACA GAAGCCAGGC CAGAACACTG 
GACTGGGATA AACAGGGTTC CACAGCGGTA CACCCGCAAG AAGCCACACA GAGCTCCTGG 
CAAGAGGAGG TCACGCAGGG CCCACACTCA TTCCAGAGAA GGATCACCAC CATCCAAGGG 
CCGGAGCCTG GTGCGCTTCA GGAATACGAG CAGGTGCTGG TGTCTACCAG GGAGCATGTG 
CAGAGGGGGC CACCTGAGAC CGGCAGCCCC AAAGCTGGCA AGGAACCTAG CCTGTGGGCA 
CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG GGGGATGAGC TTCAGAATAT TCCAGGAGAG 
CAGGTGACGG AGGAACAATT CACAGATGAA CAGGGCAACA TTGTTACCAA GAAGATCATT 
CGCAAAGTCG TCCGGCAGGT AGACTCGTCT GGTGCCATCG ACACCCAGCA GCACGAGGAG 
GTGGAGCTAA GAGGGAGTGG ACTCCAGCCG GACCTGATAG AGGGCAGGAA GGGGGCTCAG 
ATAGTGAAGC GGGCCAGCCT GAAAAGGGGC AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264215.1
CDS	374..5932
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001277286.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277286.2

ORF Insert Sequence:

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GCCAGCAGCG AGTTCACGCC 
CGAATCACAG ACTCACCCTC AGTGAGGCAG GTGCTGGACA GAAGCCAGGC CAGAACACTG 
GACTGGGATA AACAGGGTTC CACAGCGGTA CACCCGCAAG AAGCCACACA GAGCTCCTGG 
CAAGAGGAGG TCACGCAGGG CCCACACTCA TTCCAGAGAA GGATCACCAC CATCCAAGGG 
CCGGAGCCTG GTGCGCTTCA GGAATACGAG CAGGTGCTGG TGTCTACCAG GGAGCATGTG 
CAGAGGGGGC CACCTGAGAC CGGCAGCCCC AAAGCTGGCA AGGAACCTAG CCTGTGGGCA 
CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG GGGGATGAGC TTCAGAATAT TCCAGGAGAG 
CAGGTGACGG AGGAACAATT CACAGATGAA CAGGGCAACA TTGTTACCAA GAAGATCATT 
CGCAAAGTCG TCCGGCAGGT AGACTCGTCT GGTGCCATCG ACACCCAGCA GCACGAGGAG 
GTGGAGCTAA GAGGGAGTGG ACTCCAGCCG GACCTGATAG AGGGCAGGAA GGGGGCTCAG 
ATAGTGAAGC GGGCCAGCCT GAAAAGGGGC AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001277284.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264213.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 5, mRNA.

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CloneID	OMu16933
Clone ID Related Accession (Same CDS sequence)	NM_001277284.2
Accession Version	NM_001277284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101195.1, BC079910.1, AC126445.5, AK147483.1 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_001277284.1. Transcript Variant: This variant (5) has an alternate 5' exon, lacks an alternate in-frame exon, and uses an alternate in-frame splice junction in a 3' exon compared to variant 1. The resulting isoform (5) has a shorter and distinct N-terminus, lacks an alternate internal segment, and contains an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GTAAAGCCGA TGCTGCTACC AGCTTTCTGC GGGCGGCACG CTCGGGGAAC 
CTGGACAAGG CTCTGGATCA CCTGCGCAAT GGAGTGGACA TTAACACCTG TAACCAGAAC 
GGGTTGAACG GCCTGCATCT GGCCTCCAAA GAAGGCCATG TGAAGATGGT GGTTGAACTT 
CTGCACAAAG AGATCATTCT AGAAACGACA ACCAAGAAGG GGAACACTGC TCTGCACATC 
GCTGCCCTTG CTGGTCAGGA TGAGGTGGTC CGGGAGCTGG TCAACTATGG AGCCAATGTC 
AATGCCCAGT CTCAGAAAGG CTTTACTCCC CTGTACATGG CTGCTCAGGA GAACCACTTG 
GAAGTGGTGA AATTTCTACT GGAGAATGGA GCCAATCAGA ATGTAGCCAC AGAAGATGGC 
TTCACCCCAC TGGCCGTGGC TCTACAGCAG GGTCACGAGA ATGTGGTGGC TCACCTCATC 
AACTATGGGA CGAAAGGGAA AGTGCGCCTC CCTGCCCTGC ACATCGCGGC CCGCAACGAT 
GACACACGGA CAGCCGCAGT CCTTCTGCAG AATGACCCCA ACCCAGATGT GCTTTCCAAG 
ACGGGATTCA CACCCCTCCA CATCGCAGCT CACTATGAGA ACCTCAACGT GGCCCAGTTG 
CTCCTCAACA GGGGAGCCAG CGTCAACTTC ACACCTCAGA ATGGCATCAC CCCACTACAC 
ATCGCCTCCC GCAGGGGGAA CGTGATCATG GTGAGACTCC TGCTGGACCG AGGGGCTCAG 
ATAGAAACGA GGACCAAGGA TGAATTGACA CCGCTCCACT GTGCAGCTCG GAATGGACAC 
GTGAGAATCT CAGAGATCCT GCTGGACCAC GGGGCACCCA TCCAAGCCAA AACCAAGAAT 
GGCTTGTCCC CAATCCACAT GGCCGCTCAG GGAGACCACC TCGACTGTGT CCGACTTCTA 
TTGCAATACA ATGCAGAGAT AGACGACATC ACCTTGGATC ACCTGACTCC TCTCCATGTG 
GCAGCCCACT GTGGCCACCA CCGGGTGGCT AAGGTTCTTT TGGATAAAGG GGCCAAGCCC 
AACTCCAGAG CCCTGAATGG TTTTACCCCG TTACACATCG CCTGCAAGAA GAACCACATC 
CGTGTAATGG AGTTGCTGCT GAAGACAGGA GCCTCCATCG ACGCGGTCAC TGAGTCTGGC 
CTGACACCTC TCCACGTAGC CTCCTTCATG GGACACCTTC CTATTGTGAA GAACTTACTG 
CAGCGGGGAG CGTCACCCAA TGTCTCCAAT GTGAAAGTAG AAACCCCCTT GCACATGGCA 
GCCCGAGCAG GGCATACAGA AGTGGCCAAA TATTTGCTCC AGAACAAAGC CAAAGCCAAC 
GCCAAGGCCA AGGATGACCA GACACCGCTT CACTGTGCTG CTCGAATCGG CCACACAGGC 
ATGGTGAAGC TCCTGCTGGA GAATGGTGCC AGCCCCAATC TGGCTACCAC TGCTGGCCAC 
ACACCCCTAC ACACCGCAGC CCGTGAGGGA CACGTGGACA CAGCCCTGGC CCTGCTGGAG 
AAGGAGGCAT CCCAAGCCTG CATGACCAAG AAAGGATTTA CCCCTCTGCA CGTGGCTGCT 
AAGTACGGGA AGGTACGGTT GGCGGAGCTG CTGCTGGAAC ACGATGCACA CCCCAATGCA 
GCTGGGAAGA ACGGCTTGAC TCCTCTGCAT GTGGCCGTCC ATCACAACAA CCTGGACATT 
GTCAAACTTC TTCTTCCCCG AGGTGGCTCC CCCCACAGCC CTGCCTGGAA TGGCTACACT 
CCTTTGCACA TCGCTGCCAA GCAGAACCAG ATAGAGGTGG CCCGCAGTCT ACTGCAGTAC 
GGAGGGTCAG CGAATGCAGA GTCGGTACAA GGCGTGACCC CACTTCACCT GGCTGCCCAA 
GAGGGCCACA CAGAAATGGT TGCTCTTCTC CTGTCCAAGC AAGCCAACGG CAACCTGGGG 
AACAAGAGTG GACTCACTCC CCTCCACCTG GTGTCACAAG AAGGCCATGT TCCAGTGGCA 
GACGTGCTGA TCAAACATGG CGTCACCGTG GATGCTACCA CCCGGATGGG TTACACCCCG 
CTCCATGTGG CCAGTCATTA TGGAAACATC AAGCTGGTGA AGTTCTTGCT ACAGCACCAG 
GCAGATGTCA ATGCCAAGAC CAAGCTAGGA TACAGCCCCT TGCACCAGGC AGCTCAACAA 
GGACACACAG ACATTGTGAC ATTGCTCCTG AAGAATGGTG CTTCTCCAAA TGAGGTCAGC 
TCGAACGGAA CCACACCTCT GGCAATAGCC AAACGTTTGG GCTACATCTC TGTAACAGAC 
GTGCTCAAGG TGGTCACAGA TGAAACCAGT GTGGTGTTAG TCAGTGACAA ACACCGGATG 
AGCTACCCTG AGACGGTGGA CGAGATCCTG GACGTGTCTG AAGATGAAGG TGACGAACTT 
GTCGGCTCGA AGGCTGAAAG GCGGGACTCC CGGGATGTGG GTGAGGAGAA AGAGCTGTTG 
GATTTTGTGC CGAAGCTAGA CCAAGTGGTG GAGTCTCCAG CCATCCCAAG GATCCCTTGT 
GTCACACCTG AGACCGTTGT GATCCGATCT GAAGATCAGG AACAAGCATC TAAAGAATAT 
GATGAGGATT CCCTCATTCC CAGCAGCCCG GCCACAGAGA CCTCAGACAA CATTAGCCCT 
GTGGCTAGCC CGGTCCACAC AGGGTTCCTG GTGAGCTTCA TGGTGGATGC CCGAGGGGGA 
TCCATGAGAG GAAGCCGTCA TAATGGCCTC AGGGTGGTGA TCCCTCCCCG GACATGCGCA 
GCACCCACTC GCATCACCTG CCGCCTCGTT AAGCCGCAGA AGCTGAACAC GCCACCCCCA 
CTGGCTGAGG AAGAGGGCCT AGCCAGCAGG ATCATTGCCC TGGGACCCAC GGGGGCCCAG 
TTCCTTAGCC CTGTGATCGT GGAAATCCCC CATTTTGCCT CCCATGGCCG TGGGGACCGT 
GAGCTGGTGG TTCTGAGGAG TGAAAACGGC TCTGTGTGGA AGGAGCACAA GAGTCGTTAT 
GGGGAAAGCT ACCTGGACCA GATCCTTAAT GGCATGGATG AAGAGCTGGG GAGCCTGGAG 
GAGCTGGAGA AGAAACGTGT GTGCCGCATC ATCACCACCG ACTTCCCTCT GTACTTTGTC 
ATCATGTCCC GGCTCTGCCA GGACTACGAC ACCATCGGTC CTGAAGGGGG TTCCCTGAGA 
AGCAAGCTGG TACCGCTGGT ACAGGCAACG TTCCCTGAAA ACGCTGTGAC CAAGAAAGTG 
AAGCTGGCTC TGCAGGCCCA GCCTGTCCCG GATGAGCTGG TGACCAAGCT CCTGGGTAAC 
CAGGCCACAT TCAGCCCCAT TGTGACCGTG GAGCCGAGGC GTCGGAAGTT CCACCGTCCC 
ATCGGGCTGC GAATCCCGCT CCCTCCCTCA TGGACGGACA ACCCCAGGGA CAGTGGGGAG 
GGAGACACCA CCAGCCTGCG CCTACTGTGT AGCGTCATCG GTGGAACTGA CCAAGCCCAG 
TGGGAAGATA TAACGGGAAC AACCAAACTT ATATACGCCA ATGAGTGTGC CAACTTTACC 
ACCAATGTCT CAGCCAGGTT CTGGCTGTCA GACTGTCCCC GGACTGCGGA GGCTGTGCAC 
TTTGCCACTC TCCTGTACAA AGAGCTCACA GCAGTCCCCT ACATGGCTAA GTTTGTCATT 
TTTGCCAAGA TGAACGACGC TCGGGAAGGA CGGCTCCGTT GCTACTGCAT GACGGATGAC 
AAAGTGGACA AGACCCTGGA GCAGCATGAA AACTTCGTGG AGGTGGCCCG GAGCAGGGAC 
ATAGAGGTTC TGGAAGGGAT GCCTCTGTTT GCAGAACTCT CTGGGAACTT GGTTCCTGTC 
AAGAAAGCGG CCCAACAGCG GAGCTTCCAT TTCCAGTCGT TTCGAGAGAA TCGTCTGGCC 
ATCCCCGTGA AGGTGAGGGA CAGCAGCCGC GAGCCAGGAG GGTTCTTGTC GTTCCTACGA 
AAGACAATGA AGTACGAGGA CACACAGCAC ATCCTCTGTC ACCTGAATAT CACCATGCCC 
CCCTGCACCA AGGGCAGTGG AGCAGAAGAC AGGAGAAGGA CCCTGACACC CCTGACCCTT 
CGATACAGCA TTCTCAGCGA GTCGCGGCTG GGTTTTACCA GTGACACAGA CCGTGTCGAA 
ATGAGGATGG CTGTCATCAG AGAACACCTC GGCCTAAGCT GGGCAGAGCT GGCCCGGGAG 
CTGCAGTTCA GCGTGGAAGA CATCAACCGG ATCCGTGTAG AAAACCCTAA CTCCTTGTTG 
GACCAGAGTA CAGCCTTGCT GACCCTCTGG GTGGACCGTG AAGGCGAAAA TGCAAAGATG 
GAGAATTTGT ACACAGCCCT GCGGAACATC GACAGGAGTG AGATTGTTAA CATGTTGGAG 
GGCTCCGGCA GGCAGAGCAG AAACCTCAAA CCAGAGCGGA GACATGGGGA CCGGGAGTAC 
TCATTGTCAC CCTCCCAGGT GAATGGTTAC TCCTCGCTGC AGGACGAGCT GCTGTCCCCC 
GCCTCCCTGC AGTACGCTCT CCCCTCTCCA CTGTGTGCAG ACCAGTACTG GAACGAAGTG 
GCCGTCATAG ACGCCATCCC CCTGGCGGCT ACAGAGCATG ACACCATGCT GGAGATGTCT 
GACATGCAGG TGTGGTCTGC GGGCCTCACA CCCTCCCTGG TCACTGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCTGAC GCCATACCAG AGTGGAAGTT GGAAGGGGCA 
CACTCAGAGG ACACGCAGGG CCCGGAGCTG GGCTCTCAGG ACCTGGTGGA GGACGACACA 
GTGGATTCAG ATGCCACAAA TGGCCTGGCA GATTTGCTAG GTCAGGAGGA AGGTCAGAGG 
TCAGAAAAGA AGAGGCAGGA AGTCTCAGGC ACAGAGCAGG ACACGGAGAC TGAAGTGTCT 
CTTGTTTCAG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGATTGTCG AGGGTCCCCT GGCAGACCCT 
GGGGATCTGG AAGCAGATAT TGAGTCCTTT ATGAAACTTA CTAAGGTGGA GCTAAGAGGG 
AGTGGACTCC AGCCGGACCT GATAGAGGGC AGGAAGGGGG CTCAGATAGT GAAGCGGGCC 
AGCCTGAAAA GGGGCAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264213.1
CDS	133..5796
Translation	MGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001277284.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277284.2

ORF Insert Sequence:

ATGGGCTTCT GTAAAGCCGA TGCTGCTACC AGCTTTCTGC GGGCGGCACG CTCGGGGAAC 
CTGGACAAGG CTCTGGATCA CCTGCGCAAT GGAGTGGACA TTAACACCTG TAACCAGAAC 
GGGTTGAACG GCCTGCATCT GGCCTCCAAA GAAGGCCATG TGAAGATGGT GGTTGAACTT 
CTGCACAAAG AGATCATTCT AGAAACGACA ACCAAGAAGG GGAACACTGC TCTGCACATC 
GCTGCCCTTG CTGGTCAGGA TGAGGTGGTC CGGGAGCTGG TCAACTATGG AGCCAATGTC 
AATGCCCAGT CTCAGAAAGG CTTTACTCCC CTGTACATGG CTGCTCAGGA GAACCACTTG 
GAAGTGGTGA AATTTCTACT GGAGAATGGA GCCAATCAGA ATGTAGCCAC AGAAGATGGC 
TTCACCCCAC TGGCCGTGGC TCTACAGCAG GGTCACGAGA ATGTGGTGGC TCACCTCATC 
AACTATGGGA CGAAAGGGAA AGTGCGCCTC CCTGCCCTGC ACATCGCGGC CCGCAACGAT 
GACACACGGA CAGCCGCAGT CCTTCTGCAG AATGACCCCA ACCCAGATGT GCTTTCCAAG 
ACGGGATTCA CACCCCTCCA CATCGCAGCT CACTATGAGA ACCTCAACGT GGCCCAGTTG 
CTCCTCAACA GGGGAGCCAG CGTCAACTTC ACACCTCAGA ATGGCATCAC CCCACTACAC 
ATCGCCTCCC GCAGGGGGAA CGTGATCATG GTGAGACTCC TGCTGGACCG AGGGGCTCAG 
ATAGAAACGA GGACCAAGGA TGAATTGACA CCGCTCCACT GTGCAGCTCG GAATGGACAC 
GTGAGAATCT CAGAGATCCT GCTGGACCAC GGGGCACCCA TCCAAGCCAA AACCAAGAAT 
GGCTTGTCCC CAATCCACAT GGCCGCTCAG GGAGACCACC TCGACTGTGT CCGACTTCTA 
TTGCAATACA ATGCAGAGAT AGACGACATC ACCTTGGATC ACCTGACTCC TCTCCATGTG 
GCAGCCCACT GTGGCCACCA CCGGGTGGCT AAGGTTCTTT TGGATAAAGG GGCCAAGCCC 
AACTCCAGAG CCCTGAATGG TTTTACCCCG TTACACATCG CCTGCAAGAA GAACCACATC 
CGTGTAATGG AGTTGCTGCT GAAGACAGGA GCCTCCATCG ACGCGGTCAC TGAGTCTGGC 
CTGACACCTC TCCACGTAGC CTCCTTCATG GGACACCTTC CTATTGTGAA GAACTTACTG 
CAGCGGGGAG CGTCACCCAA TGTCTCCAAT GTGAAAGTAG AAACCCCCTT GCACATGGCA 
GCCCGAGCAG GGCATACAGA AGTGGCCAAA TATTTGCTCC AGAACAAAGC CAAAGCCAAC 
GCCAAGGCCA AGGATGACCA GACACCGCTT CACTGTGCTG CTCGAATCGG CCACACAGGC 
ATGGTGAAGC TCCTGCTGGA GAATGGTGCC AGCCCCAATC TGGCTACCAC TGCTGGCCAC 
ACACCCCTAC ACACCGCAGC CCGTGAGGGA CACGTGGACA CAGCCCTGGC CCTGCTGGAG 
AAGGAGGCAT CCCAAGCCTG CATGACCAAG AAAGGATTTA CCCCTCTGCA CGTGGCTGCT 
AAGTACGGGA AGGTACGGTT GGCGGAGCTG CTGCTGGAAC ACGATGCACA CCCCAATGCA 
GCTGGGAAGA ACGGCTTGAC TCCTCTGCAT GTGGCCGTCC ATCACAACAA CCTGGACATT 
GTCAAACTTC TTCTTCCCCG AGGTGGCTCC CCCCACAGCC CTGCCTGGAA TGGCTACACT 
CCTTTGCACA TCGCTGCCAA GCAGAACCAG ATAGAGGTGG CCCGCAGTCT ACTGCAGTAC 
GGAGGGTCAG CGAATGCAGA GTCGGTACAA GGCGTGACCC CACTTCACCT GGCTGCCCAA 
GAGGGCCACA CAGAAATGGT TGCTCTTCTC CTGTCCAAGC AAGCCAACGG CAACCTGGGG 
AACAAGAGTG GACTCACTCC CCTCCACCTG GTGTCACAAG AAGGCCATGT TCCAGTGGCA 
GACGTGCTGA TCAAACATGG CGTCACCGTG GATGCTACCA CCCGGATGGG TTACACCCCG 
CTCCATGTGG CCAGTCATTA TGGAAACATC AAGCTGGTGA AGTTCTTGCT ACAGCACCAG 
GCAGATGTCA ATGCCAAGAC CAAGCTAGGA TACAGCCCCT TGCACCAGGC AGCTCAACAA 
GGACACACAG ACATTGTGAC ATTGCTCCTG AAGAATGGTG CTTCTCCAAA TGAGGTCAGC 
TCGAACGGAA CCACACCTCT GGCAATAGCC AAACGTTTGG GCTACATCTC TGTAACAGAC 
GTGCTCAAGG TGGTCACAGA TGAAACCAGT GTGGTGTTAG TCAGTGACAA ACACCGGATG 
AGCTACCCTG AGACGGTGGA CGAGATCCTG GACGTGTCTG AAGATGAAGG TGACGAACTT 
GTCGGCTCGA AGGCTGAAAG GCGGGACTCC CGGGATGTGG GTGAGGAGAA AGAGCTGTTG 
GATTTTGTGC CGAAGCTAGA CCAAGTGGTG GAGTCTCCAG CCATCCCAAG GATCCCTTGT 
GTCACACCTG AGACCGTTGT GATCCGATCT GAAGATCAGG AACAAGCATC TAAAGAATAT 
GATGAGGATT CCCTCATTCC CAGCAGCCCG GCCACAGAGA CCTCAGACAA CATTAGCCCT 
GTGGCTAGCC CGGTCCACAC AGGGTTCCTG GTGAGCTTCA TGGTGGATGC CCGAGGGGGA 
TCCATGAGAG GAAGCCGTCA TAATGGCCTC AGGGTGGTGA TCCCTCCCCG GACATGCGCA 
GCACCCACTC GCATCACCTG CCGCCTCGTT AAGCCGCAGA AGCTGAACAC GCCACCCCCA 
CTGGCTGAGG AAGAGGGCCT AGCCAGCAGG ATCATTGCCC TGGGACCCAC GGGGGCCCAG 
TTCCTTAGCC CTGTGATCGT GGAAATCCCC CATTTTGCCT CCCATGGCCG TGGGGACCGT 
GAGCTGGTGG TTCTGAGGAG TGAAAACGGC TCTGTGTGGA AGGAGCACAA GAGTCGTTAT 
GGGGAAAGCT ACCTGGACCA GATCCTTAAT GGCATGGATG AAGAGCTGGG GAGCCTGGAG 
GAGCTGGAGA AGAAACGTGT GTGCCGCATC ATCACCACCG ACTTCCCTCT GTACTTTGTC 
ATCATGTCCC GGCTCTGCCA GGACTACGAC ACCATCGGTC CTGAAGGGGG TTCCCTGAGA 
AGCAAGCTGG TACCGCTGGT ACAGGCAACG TTCCCTGAAA ACGCTGTGAC CAAGAAAGTG 
AAGCTGGCTC TGCAGGCCCA GCCTGTCCCG GATGAGCTGG TGACCAAGCT CCTGGGTAAC 
CAGGCCACAT TCAGCCCCAT TGTGACCGTG GAGCCGAGGC GTCGGAAGTT CCACCGTCCC 
ATCGGGCTGC GAATCCCGCT CCCTCCCTCA TGGACGGACA ACCCCAGGGA CAGTGGGGAG 
GGAGACACCA CCAGCCTGCG CCTACTGTGT AGCGTCATCG GTGGAACTGA CCAAGCCCAG 
TGGGAAGATA TAACGGGAAC AACCAAACTT ATATACGCCA ATGAGTGTGC CAACTTTACC 
ACCAATGTCT CAGCCAGGTT CTGGCTGTCA GACTGTCCCC GGACTGCGGA GGCTGTGCAC 
TTTGCCACTC TCCTGTACAA AGAGCTCACA GCAGTCCCCT ACATGGCTAA GTTTGTCATT 
TTTGCCAAGA TGAACGACGC TCGGGAAGGA CGGCTCCGTT GCTACTGCAT GACGGATGAC 
AAAGTGGACA AGACCCTGGA GCAGCATGAA AACTTCGTGG AGGTGGCCCG GAGCAGGGAC 
ATAGAGGTTC TGGAAGGGAT GCCTCTGTTT GCAGAACTCT CTGGGAACTT GGTTCCTGTC 
AAGAAAGCGG CCCAACAGCG GAGCTTCCAT TTCCAGTCGT TTCGAGAGAA TCGTCTGGCC 
ATCCCCGTGA AGGTGAGGGA CAGCAGCCGC GAGCCAGGAG GGTTCTTGTC GTTCCTACGA 
AAGACAATGA AGTACGAGGA CACACAGCAC ATCCTCTGTC ACCTGAATAT CACCATGCCC 
CCCTGCACCA AGGGCAGTGG AGCAGAAGAC AGGAGAAGGA CCCTGACACC CCTGACCCTT 
CGATACAGCA TTCTCAGCGA GTCGCGGCTG GGTTTTACCA GTGACACAGA CCGTGTCGAA 
ATGAGGATGG CTGTCATCAG AGAACACCTC GGCCTAAGCT GGGCAGAGCT GGCCCGGGAG 
CTGCAGTTCA GCGTGGAAGA CATCAACCGG ATCCGTGTAG AAAACCCTAA CTCCTTGTTG 
GACCAGAGTA CAGCCTTGCT GACCCTCTGG GTGGACCGTG AAGGCGAAAA TGCAAAGATG 
GAGAATTTGT ACACAGCCCT GCGGAACATC GACAGGAGTG AGATTGTTAA CATGTTGGAG 
GGCTCCGGCA GGCAGAGCAG AAACCTCAAA CCAGAGCGGA GACATGGGGA CCGGGAGTAC 
TCATTGTCAC CCTCCCAGGT GAATGGTTAC TCCTCGCTGC AGGACGAGCT GCTGTCCCCC 
GCCTCCCTGC AGTACGCTCT CCCCTCTCCA CTGTGTGCAG ACCAGTACTG GAACGAAGTG 
GCCGTCATAG ACGCCATCCC CCTGGCGGCT ACAGAGCATG ACACCATGCT GGAGATGTCT 
GACATGCAGG TGTGGTCTGC GGGCCTCACA CCCTCCCTGG TCACTGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCTGAC GCCATACCAG AGTGGAAGTT GGAAGGGGCA 
CACTCAGAGG ACACGCAGGG CCCGGAGCTG GGCTCTCAGG ACCTGGTGGA GGACGACACA 
GTGGATTCAG ATGCCACAAA TGGCCTGGCA GATTTGCTAG GTCAGGAGGA AGGTCAGAGG 
TCAGAAAAGA AGAGGCAGGA AGTCTCAGGC ACAGAGCAGG ACACGGAGAC TGAAGTGTCT 
CTTGTTTCAG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGATTGTCG AGGGTCCCCT GGCAGACCCT 
GGGGATCTGG AAGCAGATAT TGAGTCCTTT ATGAAACTTA CTAAGGTGGA GCTAAGAGGG 
AGTGGACTCC AGCCGGACCT GATAGAGGGC AGGAAGGGGG CTCAGATAGT GAAGCGGGCC 
AGCCTGAAAA GGGGCAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001277289.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264218.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 7, mRNA.

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CloneID	OMu16966
Clone ID Related Accession (Same CDS sequence)	NM_001277289.2
Accession Version	NM_001277289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147483.1, X69063.1, AK147597.1, AC126445.5 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_001277289.1. Transcript Variant: This variant (7) differs in the 5' UTR, represents use of an alternate promoter compared and initiates translation at an alternate start codon to variant 1. The encoded isoform (7) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GTCAGAGGTC AGAAAAGAAG 
AGGCAGGAAG TCTCAGGCAC AGAGCAGGAC ACGGAGACTG AAGTGTCTCT TGTTTCAGGC 
CAGCAGCGAG TTCACGCCCG AATCACAGAC TCACCCTCAG TGAGGCAGGT GCTGGACAGA 
AGCCAGGCCA GAACACTGGA CTGGGATAAA CAGGGTTCCA CAGCGGTACA CCCGCAAGAA 
GCCACACAGA GCTCCTGGCA AGAGGAGGTC ACGCAGGGCC CACACTCATT CCAGAGAAGG 
ATCACCACCA TCCAAGGGCC GGAGCCTGGT GCGCTTCAGG AATACGAGCA GGTGCTGGTG 
TCTACCAGGG AGCATGTGCA GAGGGGGCCA CCTGAGACCG GCAGCCCCAA AGCTGGCAAG 
GAACCTAGCC TGTGGGCACC TGAGAGCGCC TTCTCTCAAG AGGTGCAGGG GGATGAGCTT 
CAGAATATTC CAGGAGAGCA GGTGACGGAG GAACAATTCA CAGATGAACA GGGCAACATT 
GTTACCAAGA AGATCATTCG CAAAGTCGTC CGGCAGGTAG ACTCGTCTGG TGCCATCGAC 
ACCCAGCAGC ACGAGGAGGT GGAGCTAAGA GGGAGTGGAC TCCAGCCGGA CCTGATAGAG 
GGCAGGAAGG GGGCTCAGAT AGTGAAGCGG GCCAGCCTGA AAAGGGGCAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264218.1
CDS	374..6010
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001277289.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277289.2

ORF Insert Sequence:

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GTCAGAGGTC AGAAAAGAAG 
AGGCAGGAAG TCTCAGGCAC AGAGCAGGAC ACGGAGACTG AAGTGTCTCT TGTTTCAGGC 
CAGCAGCGAG TTCACGCCCG AATCACAGAC TCACCCTCAG TGAGGCAGGT GCTGGACAGA 
AGCCAGGCCA GAACACTGGA CTGGGATAAA CAGGGTTCCA CAGCGGTACA CCCGCAAGAA 
GCCACACAGA GCTCCTGGCA AGAGGAGGTC ACGCAGGGCC CACACTCATT CCAGAGAAGG 
ATCACCACCA TCCAAGGGCC GGAGCCTGGT GCGCTTCAGG AATACGAGCA GGTGCTGGTG 
TCTACCAGGG AGCATGTGCA GAGGGGGCCA CCTGAGACCG GCAGCCCCAA AGCTGGCAAG 
GAACCTAGCC TGTGGGCACC TGAGAGCGCC TTCTCTCAAG AGGTGCAGGG GGATGAGCTT 
CAGAATATTC CAGGAGAGCA GGTGACGGAG GAACAATTCA CAGATGAACA GGGCAACATT 
GTTACCAAGA AGATCATTCG CAAAGTCGTC CGGCAGGTAG ACTCGTCTGG TGCCATCGAC 
ACCCAGCAGC ACGAGGAGGT GGAGCTAAGA GGGAGTGGAC TCCAGCCGGA CCTGATAGAG 
GGCAGGAAGG GGGCTCAGAT AGTGAAGCGG GCCAGCCTGA AAAGGGGCAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001277281.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264210.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 4, mRNA.

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CloneID	OMu17251
Clone ID Related Accession (Same CDS sequence)	NM_001277281.2
Accession Version	NM_001277281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101195.1, M84756.1, AC126445.5, AK147483.1 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_001277281.1. Transcript Variant: This variant (4) differs in the 5' UTR, represents use of an alternate promoter, initiates translation at an alternate start codon, and lacks an alternate in-frame exon compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GTAAAGCCGA TGCTGCTACC AGCTTTCTGC GGGCGGCACG CTCGGGGAAC 
CTGGACAAGG CTCTGGATCA CCTGCGCAAT GGAGTGGACA TTAACACCTG TAACCAGAAC 
GGGTTGAACG GCCTGCATCT GGCCTCCAAA GAAGGCCATG TGAAGATGGT GGTTGAACTT 
CTGCACAAAG AGATCATTCT AGAAACGACA ACCAAGAAGG GGAACACTGC TCTGCACATC 
GCTGCCCTTG CTGGTCAGGA TGAGGTGGTC CGGGAGCTGG TCAACTATGG AGCCAATGTC 
AATGCCCAGT CTCAGAAAGG CTTTACTCCC CTGTACATGG CTGCTCAGGA GAACCACTTG 
GAAGTGGTGA AATTTCTACT GGAGAATGGA GCCAATCAGA ATGTAGCCAC AGAAGATGGC 
TTCACCCCAC TGGCCGTGGC TCTACAGCAG GGTCACGAGA ATGTGGTGGC TCACCTCATC 
AACTATGGGA CGAAAGGGAA AGTGCGCCTC CCTGCCCTGC ACATCGCGGC CCGCAACGAT 
GACACACGGA CAGCCGCAGT CCTTCTGCAG AATGACCCCA ACCCAGATGT GCTTTCCAAG 
ACGGGATTCA CACCCCTCCA CATCGCAGCT CACTATGAGA ACCTCAACGT GGCCCAGTTG 
CTCCTCAACA GGGGAGCCAG CGTCAACTTC ACACCTCAGA ATGGCATCAC CCCACTACAC 
ATCGCCTCCC GCAGGGGGAA CGTGATCATG GTGAGACTCC TGCTGGACCG AGGGGCTCAG 
ATAGAAACGA GGACCAAGGA TGAATTGACA CCGCTCCACT GTGCAGCTCG GAATGGACAC 
GTGAGAATCT CAGAGATCCT GCTGGACCAC GGGGCACCCA TCCAAGCCAA AACCAAGAAT 
GGCTTGTCCC CAATCCACAT GGCCGCTCAG GGAGACCACC TCGACTGTGT CCGACTTCTA 
TTGCAATACA ATGCAGAGAT AGACGACATC ACCTTGGATC ACCTGACTCC TCTCCATGTG 
GCAGCCCACT GTGGCCACCA CCGGGTGGCT AAGGTTCTTT TGGATAAAGG GGCCAAGCCC 
AACTCCAGAG CCCTGAATGG TTTTACCCCG TTACACATCG CCTGCAAGAA GAACCACATC 
CGTGTAATGG AGTTGCTGCT GAAGACAGGA GCCTCCATCG ACGCGGTCAC TGAGTCTGGC 
CTGACACCTC TCCACGTAGC CTCCTTCATG GGACACCTTC CTATTGTGAA GAACTTACTG 
CAGCGGGGAG CGTCACCCAA TGTCTCCAAT GTGAAAGTAG AAACCCCCTT GCACATGGCA 
GCCCGAGCAG GGCATACAGA AGTGGCCAAA TATTTGCTCC AGAACAAAGC CAAAGCCAAC 
GCCAAGGCCA AGGATGACCA GACACCGCTT CACTGTGCTG CTCGAATCGG CCACACAGGC 
ATGGTGAAGC TCCTGCTGGA GAATGGTGCC AGCCCCAATC TGGCTACCAC TGCTGGCCAC 
ACACCCCTAC ACACCGCAGC CCGTGAGGGA CACGTGGACA CAGCCCTGGC CCTGCTGGAG 
AAGGAGGCAT CCCAAGCCTG CATGACCAAG AAAGGATTTA CCCCTCTGCA CGTGGCTGCT 
AAGTACGGGA AGGTACGGTT GGCGGAGCTG CTGCTGGAAC ACGATGCACA CCCCAATGCA 
GCTGGGAAGA ACGGCTTGAC TCCTCTGCAT GTGGCCGTCC ATCACAACAA CCTGGACATT 
GTCAAACTTC TTCTTCCCCG AGGTGGCTCC CCCCACAGCC CTGCCTGGAA TGGCTACACT 
CCTTTGCACA TCGCTGCCAA GCAGAACCAG ATAGAGGTGG CCCGCAGTCT ACTGCAGTAC 
GGAGGGTCAG CGAATGCAGA GTCGGTACAA GGCGTGACCC CACTTCACCT GGCTGCCCAA 
GAGGGCCACA CAGAAATGGT TGCTCTTCTC CTGTCCAAGC AAGCCAACGG CAACCTGGGG 
AACAAGAGTG GACTCACTCC CCTCCACCTG GTGTCACAAG AAGGCCATGT TCCAGTGGCA 
GACGTGCTGA TCAAACATGG CGTCACCGTG GATGCTACCA CCCGGATGGG TTACACCCCG 
CTCCATGTGG CCAGTCATTA TGGAAACATC AAGCTGGTGA AGTTCTTGCT ACAGCACCAG 
GCAGATGTCA ATGCCAAGAC CAAGCTAGGA TACAGCCCCT TGCACCAGGC AGCTCAACAA 
GGACACACAG ACATTGTGAC ATTGCTCCTG AAGAATGGTG CTTCTCCAAA TGAGGTCAGC 
TCGAACGGAA CCACACCTCT GGCAATAGCC AAACGTTTGG GCTACATCTC TGTAACAGAC 
GTGCTCAAGG TGGTCACAGA TGAAACCAGT GTGGTGTTAG TCAGTGACAA ACACCGGATG 
AGCTACCCTG AGACGGTGGA CGAGATCCTG GACGTGTCTG AAGATGAAGG TGACGAACTT 
GTCGGCTCGA AGGCTGAAAG GCGGGACTCC CGGGATGTGG GTGAGGAGAA AGAGCTGTTG 
GATTTTGTGC CGAAGCTAGA CCAAGTGGTG GAGTCTCCAG CCATCCCAAG GATCCCTTGT 
GTCACACCTG AGACCGTTGT GATCCGATCT GAAGATCAGG AACAAGCATC TAAAGAATAT 
GATGAGGATT CCCTCATTCC CAGCAGCCCG GCCACAGAGA CCTCAGACAA CATTAGCCCT 
GTGGCTAGCC CGGTCCACAC AGGGTTCCTG GTGAGCTTCA TGGTGGATGC CCGAGGGGGA 
TCCATGAGAG GAAGCCGTCA TAATGGCCTC AGGGTGGTGA TCCCTCCCCG GACATGCGCA 
GCACCCACTC GCATCACCTG CCGCCTCGTT AAGCCGCAGA AGCTGAACAC GCCACCCCCA 
CTGGCTGAGG AAGAGGGCCT AGCCAGCAGG ATCATTGCCC TGGGACCCAC GGGGGCCCAG 
TTCCTTAGCC CTGTGATCGT GGAAATCCCC CATTTTGCCT CCCATGGCCG TGGGGACCGT 
GAGCTGGTGG TTCTGAGGAG TGAAAACGGC TCTGTGTGGA AGGAGCACAA GAGTCGTTAT 
GGGGAAAGCT ACCTGGACCA GATCCTTAAT GGCATGGATG AAGAGCTGGG GAGCCTGGAG 
GAGCTGGAGA AGAAACGTGT GTGCCGCATC ATCACCACCG ACTTCCCTCT GTACTTTGTC 
ATCATGTCCC GGCTCTGCCA GGACTACGAC ACCATCGGTC CTGAAGGGGG TTCCCTGAGA 
AGCAAGCTGG TACCGCTGGT ACAGGCAACG TTCCCTGAAA ACGCTGTGAC CAAGAAAGTG 
AAGCTGGCTC TGCAGGCCCA GCCTGTCCCG GATGAGCTGG TGACCAAGCT CCTGGGTAAC 
CAGGCCACAT TCAGCCCCAT TGTGACCGTG GAGCCGAGGC GTCGGAAGTT CCACCGTCCC 
ATCGGGCTGC GAATCCCGCT CCCTCCCTCA TGGACGGACA ACCCCAGGGA CAGTGGGGAG 
GGAGACACCA CCAGCCTGCG CCTACTGTGT AGCGTCATCG GTGGAACTGA CCAAGCCCAG 
TGGGAAGATA TAACGGGAAC AACCAAACTT ATATACGCCA ATGAGTGTGC CAACTTTACC 
ACCAATGTCT CAGCCAGGTT CTGGCTGTCA GACTGTCCCC GGACTGCGGA GGCTGTGCAC 
TTTGCCACTC TCCTGTACAA AGAGCTCACA GCAGTCCCCT ACATGGCTAA GTTTGTCATT 
TTTGCCAAGA TGAACGACGC TCGGGAAGGA CGGCTCCGTT GCTACTGCAT GACGGATGAC 
AAAGTGGACA AGACCCTGGA GCAGCATGAA AACTTCGTGG AGGTGGCCCG GAGCAGGGAC 
ATAGAGGTTC TGGAAGGGAT GCCTCTGTTT GCAGAACTCT CTGGGAACTT GGTTCCTGTC 
AAGAAAGCGG CCCAACAGCG GAGCTTCCAT TTCCAGTCGT TTCGAGAGAA TCGTCTGGCC 
ATCCCCGTGA AGGTGAGGGA CAGCAGCCGC GAGCCAGGAG GGTTCTTGTC GTTCCTACGA 
AAGACAATGA AGTACGAGGA CACACAGCAC ATCCTCTGTC ACCTGAATAT CACCATGCCC 
CCCTGCACCA AGGGCAGTGG AGCAGAAGAC AGGAGAAGGA CCCTGACACC CCTGACCCTT 
CGATACAGCA TTCTCAGCGA GTCGCGGCTG GGTTTTACCA GTGACACAGA CCGTGTCGAA 
ATGAGGATGG CTGTCATCAG AGAACACCTC GGCCTAAGCT GGGCAGAGCT GGCCCGGGAG 
CTGCAGTTCA GCGTGGAAGA CATCAACCGG ATCCGTGTAG AAAACCCTAA CTCCTTGTTG 
GACCAGAGTA CAGCCTTGCT GACCCTCTGG GTGGACCGTG AAGGCGAAAA TGCAAAGATG 
GAGAATTTGT ACACAGCCCT GCGGAACATC GACAGGAGTG AGATTGTTAA CATGTTGGAG 
GGCTCCGGCA GGCAGAGCAG AAACCTCAAA CCAGAGCGGA GACATGGGGA CCGGGAGTAC 
TCATTGTCAC CCTCCCAGGT GAATGGTTAC TCCTCGCTGC AGGACGAGCT GCTGTCCCCC 
GCCTCCCTGC AGTACGCTCT CCCCTCTCCA CTGTGTGCAG ACCAGTACTG GAACGAAGTG 
GCCGTCATAG ACGCCATCCC CCTGGCGGCT ACAGAGCATG ACACCATGCT GGAGATGTCT 
GACATGCAGG TGTGGTCTGC GGGCCTCACA CCCTCCCTGG TCACTGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCTGAC GCCATACCAG AGTGGAAGTT GGAAGGGGCA 
CACTCAGAGG ACACGCAGGG CCCGGAGCTG GGCTCTCAGG ACCTGGTGGA GGACGACACA 
GTGGATTCAG ATGCCACAAA TGGCCTGGCA GATTTGCTAG GTCAGGAGGA AGGTCAGAGG 
TCAGAAAAGA AGAGGCAGGA AGTCTCAGGC ACAGAGCAGG ACACGGAGAC TGAAGTGTCT 
CTTGTTTCAG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGGAGCTAA GAGGGAGTGG ACTCCAGCCG 
GACCTGATAG AGGGCAGGAA GGGGGCTCAG ATAGTGAAGC GGGCCAGCCT GAAAAGGGGC 
AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264210.1
CDS	133..5721
Translation	MGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001277281.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277281.2

ORF Insert Sequence:

ATGGGCTTCT GTAAAGCCGA TGCTGCTACC AGCTTTCTGC GGGCGGCACG CTCGGGGAAC 
CTGGACAAGG CTCTGGATCA CCTGCGCAAT GGAGTGGACA TTAACACCTG TAACCAGAAC 
GGGTTGAACG GCCTGCATCT GGCCTCCAAA GAAGGCCATG TGAAGATGGT GGTTGAACTT 
CTGCACAAAG AGATCATTCT AGAAACGACA ACCAAGAAGG GGAACACTGC TCTGCACATC 
GCTGCCCTTG CTGGTCAGGA TGAGGTGGTC CGGGAGCTGG TCAACTATGG AGCCAATGTC 
AATGCCCAGT CTCAGAAAGG CTTTACTCCC CTGTACATGG CTGCTCAGGA GAACCACTTG 
GAAGTGGTGA AATTTCTACT GGAGAATGGA GCCAATCAGA ATGTAGCCAC AGAAGATGGC 
TTCACCCCAC TGGCCGTGGC TCTACAGCAG GGTCACGAGA ATGTGGTGGC TCACCTCATC 
AACTATGGGA CGAAAGGGAA AGTGCGCCTC CCTGCCCTGC ACATCGCGGC CCGCAACGAT 
GACACACGGA CAGCCGCAGT CCTTCTGCAG AATGACCCCA ACCCAGATGT GCTTTCCAAG 
ACGGGATTCA CACCCCTCCA CATCGCAGCT CACTATGAGA ACCTCAACGT GGCCCAGTTG 
CTCCTCAACA GGGGAGCCAG CGTCAACTTC ACACCTCAGA ATGGCATCAC CCCACTACAC 
ATCGCCTCCC GCAGGGGGAA CGTGATCATG GTGAGACTCC TGCTGGACCG AGGGGCTCAG 
ATAGAAACGA GGACCAAGGA TGAATTGACA CCGCTCCACT GTGCAGCTCG GAATGGACAC 
GTGAGAATCT CAGAGATCCT GCTGGACCAC GGGGCACCCA TCCAAGCCAA AACCAAGAAT 
GGCTTGTCCC CAATCCACAT GGCCGCTCAG GGAGACCACC TCGACTGTGT CCGACTTCTA 
TTGCAATACA ATGCAGAGAT AGACGACATC ACCTTGGATC ACCTGACTCC TCTCCATGTG 
GCAGCCCACT GTGGCCACCA CCGGGTGGCT AAGGTTCTTT TGGATAAAGG GGCCAAGCCC 
AACTCCAGAG CCCTGAATGG TTTTACCCCG TTACACATCG CCTGCAAGAA GAACCACATC 
CGTGTAATGG AGTTGCTGCT GAAGACAGGA GCCTCCATCG ACGCGGTCAC TGAGTCTGGC 
CTGACACCTC TCCACGTAGC CTCCTTCATG GGACACCTTC CTATTGTGAA GAACTTACTG 
CAGCGGGGAG CGTCACCCAA TGTCTCCAAT GTGAAAGTAG AAACCCCCTT GCACATGGCA 
GCCCGAGCAG GGCATACAGA AGTGGCCAAA TATTTGCTCC AGAACAAAGC CAAAGCCAAC 
GCCAAGGCCA AGGATGACCA GACACCGCTT CACTGTGCTG CTCGAATCGG CCACACAGGC 
ATGGTGAAGC TCCTGCTGGA GAATGGTGCC AGCCCCAATC TGGCTACCAC TGCTGGCCAC 
ACACCCCTAC ACACCGCAGC CCGTGAGGGA CACGTGGACA CAGCCCTGGC CCTGCTGGAG 
AAGGAGGCAT CCCAAGCCTG CATGACCAAG AAAGGATTTA CCCCTCTGCA CGTGGCTGCT 
AAGTACGGGA AGGTACGGTT GGCGGAGCTG CTGCTGGAAC ACGATGCACA CCCCAATGCA 
GCTGGGAAGA ACGGCTTGAC TCCTCTGCAT GTGGCCGTCC ATCACAACAA CCTGGACATT 
GTCAAACTTC TTCTTCCCCG AGGTGGCTCC CCCCACAGCC CTGCCTGGAA TGGCTACACT 
CCTTTGCACA TCGCTGCCAA GCAGAACCAG ATAGAGGTGG CCCGCAGTCT ACTGCAGTAC 
GGAGGGTCAG CGAATGCAGA GTCGGTACAA GGCGTGACCC CACTTCACCT GGCTGCCCAA 
GAGGGCCACA CAGAAATGGT TGCTCTTCTC CTGTCCAAGC AAGCCAACGG CAACCTGGGG 
AACAAGAGTG GACTCACTCC CCTCCACCTG GTGTCACAAG AAGGCCATGT TCCAGTGGCA 
GACGTGCTGA TCAAACATGG CGTCACCGTG GATGCTACCA CCCGGATGGG TTACACCCCG 
CTCCATGTGG CCAGTCATTA TGGAAACATC AAGCTGGTGA AGTTCTTGCT ACAGCACCAG 
GCAGATGTCA ATGCCAAGAC CAAGCTAGGA TACAGCCCCT TGCACCAGGC AGCTCAACAA 
GGACACACAG ACATTGTGAC ATTGCTCCTG AAGAATGGTG CTTCTCCAAA TGAGGTCAGC 
TCGAACGGAA CCACACCTCT GGCAATAGCC AAACGTTTGG GCTACATCTC TGTAACAGAC 
GTGCTCAAGG TGGTCACAGA TGAAACCAGT GTGGTGTTAG TCAGTGACAA ACACCGGATG 
AGCTACCCTG AGACGGTGGA CGAGATCCTG GACGTGTCTG AAGATGAAGG TGACGAACTT 
GTCGGCTCGA AGGCTGAAAG GCGGGACTCC CGGGATGTGG GTGAGGAGAA AGAGCTGTTG 
GATTTTGTGC CGAAGCTAGA CCAAGTGGTG GAGTCTCCAG CCATCCCAAG GATCCCTTGT 
GTCACACCTG AGACCGTTGT GATCCGATCT GAAGATCAGG AACAAGCATC TAAAGAATAT 
GATGAGGATT CCCTCATTCC CAGCAGCCCG GCCACAGAGA CCTCAGACAA CATTAGCCCT 
GTGGCTAGCC CGGTCCACAC AGGGTTCCTG GTGAGCTTCA TGGTGGATGC CCGAGGGGGA 
TCCATGAGAG GAAGCCGTCA TAATGGCCTC AGGGTGGTGA TCCCTCCCCG GACATGCGCA 
GCACCCACTC GCATCACCTG CCGCCTCGTT AAGCCGCAGA AGCTGAACAC GCCACCCCCA 
CTGGCTGAGG AAGAGGGCCT AGCCAGCAGG ATCATTGCCC TGGGACCCAC GGGGGCCCAG 
TTCCTTAGCC CTGTGATCGT GGAAATCCCC CATTTTGCCT CCCATGGCCG TGGGGACCGT 
GAGCTGGTGG TTCTGAGGAG TGAAAACGGC TCTGTGTGGA AGGAGCACAA GAGTCGTTAT 
GGGGAAAGCT ACCTGGACCA GATCCTTAAT GGCATGGATG AAGAGCTGGG GAGCCTGGAG 
GAGCTGGAGA AGAAACGTGT GTGCCGCATC ATCACCACCG ACTTCCCTCT GTACTTTGTC 
ATCATGTCCC GGCTCTGCCA GGACTACGAC ACCATCGGTC CTGAAGGGGG TTCCCTGAGA 
AGCAAGCTGG TACCGCTGGT ACAGGCAACG TTCCCTGAAA ACGCTGTGAC CAAGAAAGTG 
AAGCTGGCTC TGCAGGCCCA GCCTGTCCCG GATGAGCTGG TGACCAAGCT CCTGGGTAAC 
CAGGCCACAT TCAGCCCCAT TGTGACCGTG GAGCCGAGGC GTCGGAAGTT CCACCGTCCC 
ATCGGGCTGC GAATCCCGCT CCCTCCCTCA TGGACGGACA ACCCCAGGGA CAGTGGGGAG 
GGAGACACCA CCAGCCTGCG CCTACTGTGT AGCGTCATCG GTGGAACTGA CCAAGCCCAG 
TGGGAAGATA TAACGGGAAC AACCAAACTT ATATACGCCA ATGAGTGTGC CAACTTTACC 
ACCAATGTCT CAGCCAGGTT CTGGCTGTCA GACTGTCCCC GGACTGCGGA GGCTGTGCAC 
TTTGCCACTC TCCTGTACAA AGAGCTCACA GCAGTCCCCT ACATGGCTAA GTTTGTCATT 
TTTGCCAAGA TGAACGACGC TCGGGAAGGA CGGCTCCGTT GCTACTGCAT GACGGATGAC 
AAAGTGGACA AGACCCTGGA GCAGCATGAA AACTTCGTGG AGGTGGCCCG GAGCAGGGAC 
ATAGAGGTTC TGGAAGGGAT GCCTCTGTTT GCAGAACTCT CTGGGAACTT GGTTCCTGTC 
AAGAAAGCGG CCCAACAGCG GAGCTTCCAT TTCCAGTCGT TTCGAGAGAA TCGTCTGGCC 
ATCCCCGTGA AGGTGAGGGA CAGCAGCCGC GAGCCAGGAG GGTTCTTGTC GTTCCTACGA 
AAGACAATGA AGTACGAGGA CACACAGCAC ATCCTCTGTC ACCTGAATAT CACCATGCCC 
CCCTGCACCA AGGGCAGTGG AGCAGAAGAC AGGAGAAGGA CCCTGACACC CCTGACCCTT 
CGATACAGCA TTCTCAGCGA GTCGCGGCTG GGTTTTACCA GTGACACAGA CCGTGTCGAA 
ATGAGGATGG CTGTCATCAG AGAACACCTC GGCCTAAGCT GGGCAGAGCT GGCCCGGGAG 
CTGCAGTTCA GCGTGGAAGA CATCAACCGG ATCCGTGTAG AAAACCCTAA CTCCTTGTTG 
GACCAGAGTA CAGCCTTGCT GACCCTCTGG GTGGACCGTG AAGGCGAAAA TGCAAAGATG 
GAGAATTTGT ACACAGCCCT GCGGAACATC GACAGGAGTG AGATTGTTAA CATGTTGGAG 
GGCTCCGGCA GGCAGAGCAG AAACCTCAAA CCAGAGCGGA GACATGGGGA CCGGGAGTAC 
TCATTGTCAC CCTCCCAGGT GAATGGTTAC TCCTCGCTGC AGGACGAGCT GCTGTCCCCC 
GCCTCCCTGC AGTACGCTCT CCCCTCTCCA CTGTGTGCAG ACCAGTACTG GAACGAAGTG 
GCCGTCATAG ACGCCATCCC CCTGGCGGCT ACAGAGCATG ACACCATGCT GGAGATGTCT 
GACATGCAGG TGTGGTCTGC GGGCCTCACA CCCTCCCTGG TCACTGCTGA GGACTCCTCT 
CTGGAGTGCA GCAAGGCTGA GGACTCTGAC GCCATACCAG AGTGGAAGTT GGAAGGGGCA 
CACTCAGAGG ACACGCAGGG CCCGGAGCTG GGCTCTCAGG ACCTGGTGGA GGACGACACA 
GTGGATTCAG ATGCCACAAA TGGCCTGGCA GATTTGCTAG GTCAGGAGGA AGGTCAGAGG 
TCAGAAAAGA AGAGGCAGGA AGTCTCAGGC ACAGAGCAGG ACACGGAGAC TGAAGTGTCT 
CTTGTTTCAG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGGAGCTAA GAGGGAGTGG ACTCCAGCCG 
GACCTGATAG AGGGCAGGAA GGGGGCTCAG ATAGTGAAGC GGGCCAGCCT GAAAAGGGGC 
AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001277280.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264209.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 3, mRNA.

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CloneID	OMu17276
Clone ID Related Accession (Same CDS sequence)	NM_001277280.2
Accession Version	NM_001277280.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ105945.1, AK158131.1, AC126445.5, AK147483.1 and AI846810.1. On Aug 10, 2013 this sequence version replaced NM_001277280.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses an alternate in-frame splice junction in a 3' exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but lacks two alternate internal segments compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164132, SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GTGACGAACT TGTCGGCTCG 
AAGGCTGAAA GGCGGGACTC CCGGGATGTG GGTGAGGAGA AAGAGCTGTT GGATTTTGTG 
CCGAAGCTAG ACCAAGTGGT GGAGTCTCCA GCCATCCCAA GGATCCCTTG TGTCACACCT 
GAGACCGTTG TGATCCGATC TGAAGATCAG GAACAAGCAT CTAAAGAATA TGATGAGGAT 
TCCCTCATTC CCAGCAGCCC GGCCACAGAG ACCTCAGACA ACATTAGCCC TGTGGCTAGC 
CCGGTCCACA CAGGGTTCCT GGTGAGCTTC ATGGTGGATG CCCGAGGGGG ATCCATGAGA 
GGAAGCCGTC ATAATGGCCT CAGGGTGGTG ATCCCTCCCC GGACATGCGC AGCACCCACT 
CGCATCACCT GCCGCCTCGT TAAGCCGCAG AAGCTGAACA CGCCACCCCC ACTGGCTGAG 
GAAGAGGGCC TAGCCAGCAG GATCATTGCC CTGGGACCCA CGGGGGCCCA GTTCCTTAGC 
CCTGTGATCG TGGAAATCCC CCATTTTGCC TCCCATGGCC GTGGGGACCG TGAGCTGGTG 
GTTCTGAGGA GTGAAAACGG CTCTGTGTGG AAGGAGCACA AGAGTCGTTA TGGGGAAAGC 
TACCTGGACC AGATCCTTAA TGGCATGGAT GAAGAGCTGG GGAGCCTGGA GGAGCTGGAG 
AAGAAACGTG TGTGCCGCAT CATCACCACC GACTTCCCTC TGTACTTTGT CATCATGTCC 
CGGCTCTGCC AGGACTACGA CACCATCGGT CCTGAAGGGG GTTCCCTGAG AAGCAAGCTG 
GTACCGCTGG TACAGGCAAC GTTCCCTGAA AACGCTGTGA CCAAGAAAGT GAAGCTGGCT 
CTGCAGGCCC AGCCTGTCCC GGATGAGCTG GTGACCAAGC TCCTGGGTAA CCAGGCCACA 
TTCAGCCCCA TTGTGACCGT GGAGCCGAGG CGTCGGAAGT TCCACCGTCC CATCGGGCTG 
CGAATCCCGC TCCCTCCCTC ATGGACGGAC AACCCCAGGG ACAGTGGGGA GGGAGACACC 
ACCAGCCTGC GCCTACTGTG TAGCGTCATC GGTGGAACTG ACCAAGCCCA GTGGGAAGAT 
ATAACGGGAA CAACCAAACT TATATACGCC AATGAGTGTG CCAACTTTAC CACCAATGTC 
TCAGCCAGGT TCTGGCTGTC AGACTGTCCC CGGACTGCGG AGGCTGTGCA CTTTGCCACT 
CTCCTGTACA AAGAGCTCAC AGCAGTCCCC TACATGGCTA AGTTTGTCAT TTTTGCCAAG 
ATGAACGACG CTCGGGAAGG ACGGCTCCGT TGCTACTGCA TGACGGATGA CAAAGTGGAC 
AAGACCCTGG AGCAGCATGA AAACTTCGTG GAGGTGGCCC GGAGCAGGGA CATAGAGGTT 
CTGGAAGGGA TGCCTCTGTT TGCAGAACTC TCTGGGAACT TGGTTCCTGT CAAGAAAGCG 
GCCCAACAGC GGAGCTTCCA TTTCCAGTCG TTTCGAGAGA ATCGTCTGGC CATCCCCGTG 
AAGGTGAGGG ACAGCAGCCG CGAGCCAGGA GGGTTCTTGT CGTTCCTACG AAAGACAATG 
AAGTACGAGG ACACACAGCA CATCCTCTGT CACCTGAATA TCACCATGCC CCCCTGCACC 
AAGGGCAGTG GAGCAGAAGA CAGGAGAAGG ACCCTGACAC CCCTGACCCT TCGATACAGC 
ATTCTCAGCG AGTCGCGGCT GGGTTTTACC AGTGACACAG ACCGTGTCGA AATGAGGATG 
GCTGTCATCA GAGAACACCT CGGCCTAAGC TGGGCAGAGC TGGCCCGGGA GCTGCAGTTC 
AGCGTGGAAG ACATCAACCG GATCCGTGTA GAAAACCCTA ACTCCTTGTT GGACCAGAGT 
ACAGCCTTGC TGACCCTCTG GGTGGACCGT GAAGGCGAAA ATGCAAAGAT GGAGAATTTG 
TACACAGCCC TGCGGAACAT CGACAGGAGT GAGATTGTTA ACATGTTGGA GGGCTCCGGC 
AGGCAGAGCA GAAACCTCAA ACCAGAGCGG AGACATGGGG ACCGGGAGTA CTCATTGTCA 
CCCTCCCAGG TGAATGGCCA GCAGCGAGTT CACGCCCGAA TCACAGACTC ACCCTCAGTG 
AGGCAGGTGC TGGACAGAAG CCAGGCCAGA ACACTGGACT GGGATAAACA GGGTTCCACA 
GCGGTACACC CGCAAGAAGC CACACAGAGC TCCTGGCAAG AGGAGGTCAC GCAGGGCCCA 
CACTCATTCC AGAGAAGGAT CACCACCATC CAAGGGCCGG AGCCTGGTGC GCTTCAGGAA 
TACGAGCAGG TGCTGGTGTC TACCAGGGAG CATGTGCAGA GGGGGCCACC TGAGACCGGC 
AGCCCCAAAG CTGGCAAGGA ACCTAGCCTG TGGGCACCTG AGAGCGCCTT CTCTCAAGAG 
GTGCAGGGGG ATGAGCTTCA GAATATTCCA GGAGAGCAGG TGACGGAGGA ACAATTCACA 
GATGAACAGG GCAACATTGT TACCAAGAAG ATCATTCGCA AAGTCGTCCG GCAGGTAGAC 
TCGTCTGGTG CCATCGACAC CCAGCAGCAC GAGGAGGTGG AGCTAAGAGG GAGTGGACTC 
CAGCCGGACC TGATAGAGGG CAGGAAGGGG GCTCAGATAG TGAAGCGGGC CAGCCTGAAA 
AGGGGCAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264209.1
CDS	301..5535
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001277280.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277280.2

ORF Insert Sequence:

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GTGACGAACT TGTCGGCTCG 
AAGGCTGAAA GGCGGGACTC CCGGGATGTG GGTGAGGAGA AAGAGCTGTT GGATTTTGTG 
CCGAAGCTAG ACCAAGTGGT GGAGTCTCCA GCCATCCCAA GGATCCCTTG TGTCACACCT 
GAGACCGTTG TGATCCGATC TGAAGATCAG GAACAAGCAT CTAAAGAATA TGATGAGGAT 
TCCCTCATTC CCAGCAGCCC GGCCACAGAG ACCTCAGACA ACATTAGCCC TGTGGCTAGC 
CCGGTCCACA CAGGGTTCCT GGTGAGCTTC ATGGTGGATG CCCGAGGGGG ATCCATGAGA 
GGAAGCCGTC ATAATGGCCT CAGGGTGGTG ATCCCTCCCC GGACATGCGC AGCACCCACT 
CGCATCACCT GCCGCCTCGT TAAGCCGCAG AAGCTGAACA CGCCACCCCC ACTGGCTGAG 
GAAGAGGGCC TAGCCAGCAG GATCATTGCC CTGGGACCCA CGGGGGCCCA GTTCCTTAGC 
CCTGTGATCG TGGAAATCCC CCATTTTGCC TCCCATGGCC GTGGGGACCG TGAGCTGGTG 
GTTCTGAGGA GTGAAAACGG CTCTGTGTGG AAGGAGCACA AGAGTCGTTA TGGGGAAAGC 
TACCTGGACC AGATCCTTAA TGGCATGGAT GAAGAGCTGG GGAGCCTGGA GGAGCTGGAG 
AAGAAACGTG TGTGCCGCAT CATCACCACC GACTTCCCTC TGTACTTTGT CATCATGTCC 
CGGCTCTGCC AGGACTACGA CACCATCGGT CCTGAAGGGG GTTCCCTGAG AAGCAAGCTG 
GTACCGCTGG TACAGGCAAC GTTCCCTGAA AACGCTGTGA CCAAGAAAGT GAAGCTGGCT 
CTGCAGGCCC AGCCTGTCCC GGATGAGCTG GTGACCAAGC TCCTGGGTAA CCAGGCCACA 
TTCAGCCCCA TTGTGACCGT GGAGCCGAGG CGTCGGAAGT TCCACCGTCC CATCGGGCTG 
CGAATCCCGC TCCCTCCCTC ATGGACGGAC AACCCCAGGG ACAGTGGGGA GGGAGACACC 
ACCAGCCTGC GCCTACTGTG TAGCGTCATC GGTGGAACTG ACCAAGCCCA GTGGGAAGAT 
ATAACGGGAA CAACCAAACT TATATACGCC AATGAGTGTG CCAACTTTAC CACCAATGTC 
TCAGCCAGGT TCTGGCTGTC AGACTGTCCC CGGACTGCGG AGGCTGTGCA CTTTGCCACT 
CTCCTGTACA AAGAGCTCAC AGCAGTCCCC TACATGGCTA AGTTTGTCAT TTTTGCCAAG 
ATGAACGACG CTCGGGAAGG ACGGCTCCGT TGCTACTGCA TGACGGATGA CAAAGTGGAC 
AAGACCCTGG AGCAGCATGA AAACTTCGTG GAGGTGGCCC GGAGCAGGGA CATAGAGGTT 
CTGGAAGGGA TGCCTCTGTT TGCAGAACTC TCTGGGAACT TGGTTCCTGT CAAGAAAGCG 
GCCCAACAGC GGAGCTTCCA TTTCCAGTCG TTTCGAGAGA ATCGTCTGGC CATCCCCGTG 
AAGGTGAGGG ACAGCAGCCG CGAGCCAGGA GGGTTCTTGT CGTTCCTACG AAAGACAATG 
AAGTACGAGG ACACACAGCA CATCCTCTGT CACCTGAATA TCACCATGCC CCCCTGCACC 
AAGGGCAGTG GAGCAGAAGA CAGGAGAAGG ACCCTGACAC CCCTGACCCT TCGATACAGC 
ATTCTCAGCG AGTCGCGGCT GGGTTTTACC AGTGACACAG ACCGTGTCGA AATGAGGATG 
GCTGTCATCA GAGAACACCT CGGCCTAAGC TGGGCAGAGC TGGCCCGGGA GCTGCAGTTC 
AGCGTGGAAG ACATCAACCG GATCCGTGTA GAAAACCCTA ACTCCTTGTT GGACCAGAGT 
ACAGCCTTGC TGACCCTCTG GGTGGACCGT GAAGGCGAAA ATGCAAAGAT GGAGAATTTG 
TACACAGCCC TGCGGAACAT CGACAGGAGT GAGATTGTTA ACATGTTGGA GGGCTCCGGC 
AGGCAGAGCA GAAACCTCAA ACCAGAGCGG AGACATGGGG ACCGGGAGTA CTCATTGTCA 
CCCTCCCAGG TGAATGGCCA GCAGCGAGTT CACGCCCGAA TCACAGACTC ACCCTCAGTG 
AGGCAGGTGC TGGACAGAAG CCAGGCCAGA ACACTGGACT GGGATAAACA GGGTTCCACA 
GCGGTACACC CGCAAGAAGC CACACAGAGC TCCTGGCAAG AGGAGGTCAC GCAGGGCCCA 
CACTCATTCC AGAGAAGGAT CACCACCATC CAAGGGCCGG AGCCTGGTGC GCTTCAGGAA 
TACGAGCAGG TGCTGGTGTC TACCAGGGAG CATGTGCAGA GGGGGCCACC TGAGACCGGC 
AGCCCCAAAG CTGGCAAGGA ACCTAGCCTG TGGGCACCTG AGAGCGCCTT CTCTCAAGAG 
GTGCAGGGGG ATGAGCTTCA GAATATTCCA GGAGAGCAGG TGACGGAGGA ACAATTCACA 
GATGAACAGG GCAACATTGT TACCAAGAAG ATCATTCGCA AAGTCGTCCG GCAGGTAGAC 
TCGTCTGGTG CCATCGACAC CCAGCAGCAC GAGGAGGTGG AGCTAAGAGG GAGTGGACTC 
CAGCCGGACC TGATAGAGGG CAGGAAGGGG GCTCAGATAG TGAAGCGGGC CAGCCTGAAA 
AGGGGCAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508989.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509052.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X1, mRNA.

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CloneID	OMu40540
Clone ID Related Accession (Same CDS sequence)	XM_006508989.4 , XM_006508989.3
Accession Version	XM_006508989.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGTGGAG 
CTAAGAGGGA GTGGACTCCA GCCGGACCTG ATAGAGGGCA GGAAGGGGGC TCAGATAGTG 
AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509052.1
CDS	350..6202
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508989.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508989.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGTGGAG 
CTAAGAGGGA GTGGACTCCA GCCGGACCTG ATAGAGGGCA GGAAGGGGGC TCAGATAGTG 
AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508990.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509053.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X2, mRNA.

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CloneID	OMu40541
Clone ID Related Accession (Same CDS sequence)	XM_006508990.2
Accession Version	XM_006508990.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GAACTGCTCA CATATCTATA 
ATGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAGTC TCCAGCCATC 
CCAAGGATCC CTTGTGTCAC ACCTGAGACC GTTGTGATCC GATCTGAAGA TCAGGAACAA 
GCATCTAAAG AATATGATGA GGATTCCCTC ATTCCCAGCA GCCCGGCCAC AGAGACCTCA 
GACAACATTA GCCCTGTGGC TAGCCCGGTC CACACAGGGT TCCTGGTGAG CTTCATGGTG 
GATGCCCGAG GGGGATCCAT GAGAGGAAGC CGTCATAATG GCCTCAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG 
AACACGCCAC CCCCACTGGC TGAGGAAGAG GGCCTAGCCA GCAGGATCAT TGCCCTGGGA 
CCCACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCATTT TGCCTCCCAT 
GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG 
CACAAGAGTC GTTATGGGGA AAGCTACCTG GACCAGATCC TTAATGGCAT GGATGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA CGTGTGTGCC GCATCATCAC CACCGACTTC 
CCTCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGTTCCC TGAGAAGCAA GCTGGTACCG CTGGTACAGG CAACGTTCCC TGAAAACGCT 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCGGATGA GCTGGTGACC 
AAGCTCCTGG GTAACCAGGC CACATTCAGC CCCATTGTGA CCGTGGAGCC GAGGCGTCGG 
AAGTTCCACC GTCCCATCGG GCTGCGAATC CCGCTCCCTC CCTCATGGAC GGACAACCCC 
AGGGACAGTG GGGAGGGAGA CACCACCAGC CTGCGCCTAC TGTGTAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AACTTATATA CGCCAATGAG 
TGTGCCAACT TTACCACCAA TGTCTCAGCC AGGTTCTGGC TGTCAGACTG TCCCCGGACT 
GCGGAGGCTG TGCACTTTGC CACTCTCCTG TACAAAGAGC TCACAGCAGT CCCCTACATG 
GCTAAGTTTG TCATTTTTGC CAAGATGAAC GACGCTCGGG AAGGACGGCT CCGTTGCTAC 
TGCATGACGG ATGACAAAGT GGACAAGACC CTGGAGCAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTGGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTTC CTGTCAAGAA AGCGGCCCAA CAGCGGAGCT TCCATTTCCA GTCGTTTCGA 
GAGAATCGTC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TACGAAAGAC AATGAAGTAC GAGGACACAC AGCACATCCT CTGTCACCTG 
AATATCACCA TGCCCCCCTG CACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACACCCCTGA CCCTTCGATA CAGCATTCTC AGCGAGTCGC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TCGAAATGAG GATGGCTGTC ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA 
GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG GAAGACATCA ACCGGATCCG TGTAGAAAAC 
CCTAACTCCT TGTTGGACCA GAGTACAGCC TTGCTGACCC TCTGGGTGGA CCGTGAAGGC 
GAAAATGCAA AGATGGAGAA TTTGTACACA GCCCTGCGGA ACATCGACAG GAGTGAGATT 
GTTAACATGT TGGAGGGCTC CGGCAGGCAG AGCAGAAACC TCAAACCAGA GCGGAGACAT 
GGGGACCGGG AGTACTCATT GTCACCCTCC CAGGTGAATG GTTACTCCTC GCTGCAGGAC 
GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC GCTCTCCCCT CTCCACTGTG TGCAGACCAG 
TACTGGAACG AAGTGGCCGT CATAGACGCC ATCCCCCTGG CGGCTACAGA GCATGACACC 
ATGCTGGAGA TGTCTGACAT GCAGGTGTGG TCTGCGGGCC TCACACCCTC CCTGGTCACT 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CTGACGCCAT ACCAGAGTGG 
AAGTTGGAAG GGGCACACTC AGAGGACACG CAGGGCCCGG AGCTGGGCTC TCAGGACCTG 
GTGGAGGACG ACACAGTGGA TTCAGATGCC ACAAATGGCC TGGCAGATTT GCTAGGTCAG 
GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG CAGGAAGTCT CAGGCACAGA GCAGGACACG 
GAGACTGAAG TGTCTCTTGT TTCAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CACTGGACTG GGATAAACAG 
GGTTCCACAG CGGTACACCC GCAAGAAGCC ACACAGAGCT CCTGGCAAGA GGAGGTCACG 
CAGGGCCCAC ACTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCTGGTGCG 
CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGACCGGCA GCCCCAAAGC TGGCAAGGAA CCTAGCCTGT GGGCACCTGA GAGCGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACGGAGGAA 
CAATTCACAG ATGAACAGGG CAACATTGTT ACCAAGAAGA TCATTCGCAA AGTCGTCCGG 
CAGGTAGACT CGTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGTGAT TGTCGAGGGT 
CCCCTGGCAG ACCCTGGGGA TCTGGAAGCA GATATTGAGT CCTTTATGAA ACTTACTAAG 
GTGGAGCTAA GAGGGAGTGG ACTCCAGCCG GACCTGATAG AGGGCAGGAA GGGGGCTCAG 
ATAGTGAAGC GGGCCAGCCT GAAAAGGGGC AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509053.1
CDS	301..6099
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508990.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508990.2

ORF Insert Sequence:

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GAACTGCTCA CATATCTATA 
ATGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAGTC TCCAGCCATC 
CCAAGGATCC CTTGTGTCAC ACCTGAGACC GTTGTGATCC GATCTGAAGA TCAGGAACAA 
GCATCTAAAG AATATGATGA GGATTCCCTC ATTCCCAGCA GCCCGGCCAC AGAGACCTCA 
GACAACATTA GCCCTGTGGC TAGCCCGGTC CACACAGGGT TCCTGGTGAG CTTCATGGTG 
GATGCCCGAG GGGGATCCAT GAGAGGAAGC CGTCATAATG GCCTCAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG 
AACACGCCAC CCCCACTGGC TGAGGAAGAG GGCCTAGCCA GCAGGATCAT TGCCCTGGGA 
CCCACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCATTT TGCCTCCCAT 
GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG 
CACAAGAGTC GTTATGGGGA AAGCTACCTG GACCAGATCC TTAATGGCAT GGATGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA CGTGTGTGCC GCATCATCAC CACCGACTTC 
CCTCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGTTCCC TGAGAAGCAA GCTGGTACCG CTGGTACAGG CAACGTTCCC TGAAAACGCT 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCGGATGA GCTGGTGACC 
AAGCTCCTGG GTAACCAGGC CACATTCAGC CCCATTGTGA CCGTGGAGCC GAGGCGTCGG 
AAGTTCCACC GTCCCATCGG GCTGCGAATC CCGCTCCCTC CCTCATGGAC GGACAACCCC 
AGGGACAGTG GGGAGGGAGA CACCACCAGC CTGCGCCTAC TGTGTAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AACTTATATA CGCCAATGAG 
TGTGCCAACT TTACCACCAA TGTCTCAGCC AGGTTCTGGC TGTCAGACTG TCCCCGGACT 
GCGGAGGCTG TGCACTTTGC CACTCTCCTG TACAAAGAGC TCACAGCAGT CCCCTACATG 
GCTAAGTTTG TCATTTTTGC CAAGATGAAC GACGCTCGGG AAGGACGGCT CCGTTGCTAC 
TGCATGACGG ATGACAAAGT GGACAAGACC CTGGAGCAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTGGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTTC CTGTCAAGAA AGCGGCCCAA CAGCGGAGCT TCCATTTCCA GTCGTTTCGA 
GAGAATCGTC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TACGAAAGAC AATGAAGTAC GAGGACACAC AGCACATCCT CTGTCACCTG 
AATATCACCA TGCCCCCCTG CACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACACCCCTGA CCCTTCGATA CAGCATTCTC AGCGAGTCGC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TCGAAATGAG GATGGCTGTC ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA 
GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG GAAGACATCA ACCGGATCCG TGTAGAAAAC 
CCTAACTCCT TGTTGGACCA GAGTACAGCC TTGCTGACCC TCTGGGTGGA CCGTGAAGGC 
GAAAATGCAA AGATGGAGAA TTTGTACACA GCCCTGCGGA ACATCGACAG GAGTGAGATT 
GTTAACATGT TGGAGGGCTC CGGCAGGCAG AGCAGAAACC TCAAACCAGA GCGGAGACAT 
GGGGACCGGG AGTACTCATT GTCACCCTCC CAGGTGAATG GTTACTCCTC GCTGCAGGAC 
GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC GCTCTCCCCT CTCCACTGTG TGCAGACCAG 
TACTGGAACG AAGTGGCCGT CATAGACGCC ATCCCCCTGG CGGCTACAGA GCATGACACC 
ATGCTGGAGA TGTCTGACAT GCAGGTGTGG TCTGCGGGCC TCACACCCTC CCTGGTCACT 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CTGACGCCAT ACCAGAGTGG 
AAGTTGGAAG GGGCACACTC AGAGGACACG CAGGGCCCGG AGCTGGGCTC TCAGGACCTG 
GTGGAGGACG ACACAGTGGA TTCAGATGCC ACAAATGGCC TGGCAGATTT GCTAGGTCAG 
GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG CAGGAAGTCT CAGGCACAGA GCAGGACACG 
GAGACTGAAG TGTCTCTTGT TTCAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CACTGGACTG GGATAAACAG 
GGTTCCACAG CGGTACACCC GCAAGAAGCC ACACAGAGCT CCTGGCAAGA GGAGGTCACG 
CAGGGCCCAC ACTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCTGGTGCG 
CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGACCGGCA GCCCCAAAGC TGGCAAGGAA CCTAGCCTGT GGGCACCTGA GAGCGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACGGAGGAA 
CAATTCACAG ATGAACAGGG CAACATTGTT ACCAAGAAGA TCATTCGCAA AGTCGTCCGG 
CAGGTAGACT CGTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGTGAT TGTCGAGGGT 
CCCCTGGCAG ACCCTGGGGA TCTGGAAGCA GATATTGAGT CCTTTATGAA ACTTACTAAG 
GTGGAGCTAA GAGGGAGTGG ACTCCAGCCG GACCTGATAG AGGGCAGGAA GGGGGCTCAG 
ATAGTGAAGC GGGCCAGCCT GAAAAGGGGC AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508991.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509054.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X3, mRNA.

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CloneID	OMu40542
Clone ID Related Accession (Same CDS sequence)	XM_006508991.3 , XM_006508991.4
Accession Version	XM_006508991.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGATCAC 
ACCTCAACAC CCAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509054.1
CDS	350..6130
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508991.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508991.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGATTGTCGA GGGTCCCCTG 
GCAGACCCTG GGGATCTGGA AGCAGATATT GAGTCCTTTA TGAAACTTAC TAAGGATCAC 
ACCTCAACAC CCAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508992.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509055.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X4, mRNA.

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CloneID	OMu40543
Clone ID Related Accession (Same CDS sequence)	XM_006508992.4 , XM_006508992.3
Accession Version	XM_006508992.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGGAGCTAAG AGGGAGTGGA 
CTCCAGCCGG ACCTGATAGA GGGCAGGAAG GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG 
AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509055.1
CDS	350..6127
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508992.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508992.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG TGGAGCTAAG AGGGAGTGGA 
CTCCAGCCGG ACCTGATAGA GGGCAGGAAG GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG 
AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508993.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509056.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X5, mRNA.

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CloneID	OMu40544
Clone ID Related Accession (Same CDS sequence)	XM_006508993.4 , XM_006508993.3
Accession Version	XM_006508993.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGCCAGCAGC GAGTTCACGC CCGAATCACA GACTCACCCT CAGTGAGGCA GGTGCTGGAC 
AGAAGCCAGG CCAGAACACT GGACTGGGAT AAACAGGGTT CCACAGCGGT ACACCCGCAA 
GAAGCCACAC AGAGCTCCTG GCAAGAGGAG GTCACGCAGG GCCCACACTC ATTCCAGAGA 
AGGATCACCA CCATCCAAGG GCCGGAGCCT GGTGCGCTTC AGGAATACGA GCAGGTGCTG 
GTGTCTACCA GGGAGCATGT GCAGAGGGGG CCACCTGAGA CCGGCAGCCC CAAAGCTGGC 
AAGGAACCTA GCCTGTGGGC ACCTGAGAGC GCCTTCTCTC AAGAGGTGCA GGGGGATGAG 
CTTCAGAATA TTCCAGGAGA GCAGGTGACG GAGGAACAAT TCACAGATGA ACAGGGCAAC 
ATTGTTACCA AGAAGATCAT TCGCAAAGTC GTCCGGCAGG TAGACTCGTC TGGTGCCATC 
GACACCCAGC AGCACGAGGA GGTGATTGTC GAGGGTCCCC TGGCAGACCC TGGGGATCTG 
GAAGCAGATA TTGAGTCCTT TATGAAACTT ACTAAGGTGG AGCTAAGAGG GAGTGGACTC 
CAGCCGGACC TGATAGAGGG CAGGAAGGGG GCTCAGATAG TGAAGCGGGC CAGCCTGAAA 
AGGGGCAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509056.1
CDS	350..6124
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508993.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508993.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGCCAGCAGC GAGTTCACGC CCGAATCACA GACTCACCCT CAGTGAGGCA GGTGCTGGAC 
AGAAGCCAGG CCAGAACACT GGACTGGGAT AAACAGGGTT CCACAGCGGT ACACCCGCAA 
GAAGCCACAC AGAGCTCCTG GCAAGAGGAG GTCACGCAGG GCCCACACTC ATTCCAGAGA 
AGGATCACCA CCATCCAAGG GCCGGAGCCT GGTGCGCTTC AGGAATACGA GCAGGTGCTG 
GTGTCTACCA GGGAGCATGT GCAGAGGGGG CCACCTGAGA CCGGCAGCCC CAAAGCTGGC 
AAGGAACCTA GCCTGTGGGC ACCTGAGAGC GCCTTCTCTC AAGAGGTGCA GGGGGATGAG 
CTTCAGAATA TTCCAGGAGA GCAGGTGACG GAGGAACAAT TCACAGATGA ACAGGGCAAC 
ATTGTTACCA AGAAGATCAT TCGCAAAGTC GTCCGGCAGG TAGACTCGTC TGGTGCCATC 
GACACCCAGC AGCACGAGGA GGTGATTGTC GAGGGTCCCC TGGCAGACCC TGGGGATCTG 
GAAGCAGATA TTGAGTCCTT TATGAAACTT ACTAAGGTGG AGCTAAGAGG GAGTGGACTC 
CAGCCGGACC TGATAGAGGG CAGGAAGGGG GCTCAGATAG TGAAGCGGGC CAGCCTGAAA 
AGGGGCAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508994.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509057.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X6, mRNA.

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CloneID	OMu40545
Clone ID Related Accession (Same CDS sequence)	XM_006508994.4 , XM_006508994.3
Accession Version	XM_006508994.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGTGGAG CTAAGAGGGA GTGGACTCCA GCCGGACCTG 
ATAGAGGGCA GGAAGGGGGC TCAGATAGTG AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509057.1
CDS	350..6112
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508994.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508994.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGATTGTCGA GGGTCCCCTG GCAGACCCTG GGGATCTGGA AGCAGATATT 
GAGTCCTTTA TGAAACTTAC TAAGGTGGAG CTAAGAGGGA GTGGACTCCA GCCGGACCTG 
ATAGAGGGCA GGAAGGGGGC TCAGATAGTG AAGCGGGCCA GCCTGAAAAG GGGCAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508995.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509058.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X7, mRNA.

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CloneID	OMu40546
Clone ID Related Accession (Same CDS sequence)	XM_006508995.4 , XM_006508995.3
Accession Version	XM_006508995.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGTGAT TGTCGAGGGT CCCCTGGCAG ACCCTGGGGA TCTGGAAGCA 
GATATTGAGT CCTTTATGAA ACTTACTAAG GATCACACCT CAACACCCAA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509058.1
CDS	350..6106
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508995.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508995.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGTGAT TGTCGAGGGT CCCCTGGCAG ACCCTGGGGA TCTGGAAGCA 
GATATTGAGT CCTTTATGAA ACTTACTAAG GATCACACCT CAACACCCAA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508996.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509059.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X8, mRNA.

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CloneID	OMu40547
Clone ID Related Accession (Same CDS sequence)	XM_006508996.3 , XM_006508996.2
Accession Version	XM_006508996.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GTCAGAGGTC AGAAAAGAAG 
AGGCAGGAAG TCTCAGGCAC AGAGCAGGAC ACGGAGACTG AAGTGTCTCT TGTTTCAGGC 
CAGCAGCGAG TTCACGCCCG AATCACAGAC TCACCCTCAG TGAGGCAGGT GCTGGACAGA 
AGCCAGGCCA GAACACTGGA CTGGGATAAA CAGGGTTCCA CAGCGGTACA CCCGCAAGAA 
GCCACACAGA GCTCCTGGCA AGAGGAGGTC ACGCAGGGCC CACACTCATT CCAGAGAAGG 
ATCACCACCA TCCAAGGGCC GGAGCCTGGT GCGCTTCAGG AATACGAGCA GGTGCTGGTG 
TCTACCAGGG AGCATGTGCA GAGGGGGCCA CCTGAGACCG GCAGCCCCAA AGCTGGCAAG 
GAACCTAGCC TGTGGGCACC TGAGAGCGCC TTCTCTCAAG AGGTGCAGGG GGATGAGCTT 
CAGAATATTC CAGGAGAGCA GGTGACGGAG GAACAATTCA CAGATGAACA GGGCAACATT 
GTTACCAAGA AGATCATTCG CAAAGTCGTC CGGCAGGTAG ACTCGTCTGG TGCCATCGAC 
ACCCAGCAGC ACGAGGAGGT GATTGTCGAG GGTCCCCTGG CAGACCCTGG GGATCTGGAA 
GCAGATATTG AGTCCTTTAT GAAACTTACT AAGGTGGAGC TAAGAGGGAG TGGACTCCAG 
CCGGACCTGA TAGAGGGCAG GAAGGGGGCT CAGATAGTGA AGCGGGCCAG CCTGAAAAGG 
GGCAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509059.1
CDS	315..6026
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508996.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508996.2

ORF Insert Sequence:

ATGGCAGAGC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
CTGCGGGCGG CACGCTCGGG GAACCTGGAC AAGGCTCTGG ATCACCTGCG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AACGGCCTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC GACAACCAAG 
AAGGGGAACA CTGCTCTGCA CATCGCTGCC CTTGCTGGTC AGGATGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCTCAGA AAGGCTTTAC TCCCCTGTAC 
ATGGCTGCTC AGGAGAACCA CTTGGAAGTG GTGAAATTTC TACTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAAGA TGGCTTCACC CCACTGGCCG TGGCTCTACA GCAGGGTCAC 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGGACGAAAG GGAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCTCACTAT 
GAGAACCTCA ACGTGGCCCA GTTGCTCCTC AACAGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGCA TCACCCCACT ACACATCGCC TCCCGCAGGG GGAACGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAATT GACACCGCTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCAATCC ACATGGCCGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAATGCAG AGATAGACGA CATCACCTTG 
GATCACCTGA CTCCTCTCCA TGTGGCAGCC CACTGTGGCC ACCACCGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCCAACTCC AGAGCCCTGA ATGGTTTTAC CCCGTTACAC 
ATCGCCTGCA AGAAGAACCA CATCCGTGTA ATGGAGTTGC TGCTGAAGAC AGGAGCCTCC 
ATCGACGCGG TCACTGAGTC TGGCCTGACA CCTCTCCACG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ACTGCAGCGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CCTTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAACGCCAAG GCCAAGGATG ACCAGACACC GCTTCACTGT 
GCTGCTCGAA TCGGCCACAC AGGCATGGTG AAGCTCCTGC TGGAGAATGG TGCCAGCCCC 
AATCTGGCTA CCACTGCTGG CCACACACCC CTACACACCG CAGCCCGTGA GGGACACGTG 
GACACAGCCC TGGCCCTGCT GGAGAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TGCACGTGGC TGCTAAGTAC GGGAAGGTAC GGTTGGCGGA GCTGCTGCTG 
GAACACGATG CACACCCCAA TGCAGCTGGG AAGAACGGCT TGACTCCTCT GCATGTGGCC 
GTCCATCACA ACAACCTGGA CATTGTCAAA CTTCTTCTTC CCCGAGGTGG CTCCCCCCAC 
AGCCCTGCCT GGAATGGCTA CACTCCTTTG CACATCGCTG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA GTACGGAGGG TCAGCGAATG CAGAGTCGGT ACAAGGCGTG 
ACCCCACTTC ACCTGGCTGC CCAAGAGGGC CACACAGAAA TGGTTGCTCT TCTCCTGTCC 
AAGCAAGCCA ACGGCAACCT GGGGAACAAG AGTGGACTCA CTCCCCTCCA CCTGGTGTCA 
CAAGAAGGCC ATGTTCCAGT GGCAGACGTG CTGATCAAAC ATGGCGTCAC CGTGGATGCT 
ACCACCCGGA TGGGTTACAC CCCGCTCCAT GTGGCCAGTC ATTATGGAAA CATCAAGCTG 
GTGAAGTTCT TGCTACAGCA CCAGGCAGAT GTCAATGCCA AGACCAAGCT AGGATACAGC 
CCCTTGCACC AGGCAGCTCA ACAAGGACAC ACAGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACGG TGGACGAGAT CCTGGACGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT ATAATGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA GTCTCCAGCC ATCCCAAGGA TCCCTTGTGT CACACCTGAG 
ACCGTTGTGA TCCGATCTGA AGATCAGGAA CAAGCATCTA AAGAATATGA TGAGGATTCC 
CTCATTCCCA GCAGCCCGGC CACAGAGACC TCAGACAACA TTAGCCCTGT GGCTAGCCCG 
GTCCACACAG GGTTCCTGGT GAGCTTCATG GTGGATGCCC GAGGGGGATC CATGAGAGGA 
AGCCGTCATA ATGGCCTCAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTCGTTAA GCCGCAGAAG CTGAACACGC CACCCCCACT GGCTGAGGAA 
GAGGGCCTAG CCAGCAGGAT CATTGCCCTG GGACCCACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA TTTTGCCTCC CATGGCCGTG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGTG AAAACGGCTC TGTGTGGAAG GAGCACAAGA GTCGTTATGG GGAAAGCTAC 
CTGGACCAGA TCCTTAATGG CATGGATGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAACGTGTGT GCCGCATCAT CACCACCGAC TTCCCTCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGTT CCCTGAGAAG CAAGCTGGTA 
CCGCTGGTAC AGGCAACGTT CCCTGAAAAC GCTGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCGGA TGAGCTGGTG ACCAAGCTCC TGGGTAACCA GGCCACATTC 
AGCCCCATTG TGACCGTGGA GCCGAGGCGT CGGAAGTTCC ACCGTCCCAT CGGGCTGCGA 
ATCCCGCTCC CTCCCTCATG GACGGACAAC CCCAGGGACA GTGGGGAGGG AGACACCACC 
AGCCTGCGCC TACTGTGTAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAACTTAT ATACGCCAAT GAGTGTGCCA ACTTTACCAC CAATGTCTCA 
GCCAGGTTCT GGCTGTCAGA CTGTCCCCGG ACTGCGGAGG CTGTGCACTT TGCCACTCTC 
CTGTACAAAG AGCTCACAGC AGTCCCCTAC ATGGCTAAGT TTGTCATTTT TGCCAAGATG 
AACGACGCTC GGGAAGGACG GCTCCGTTGC TACTGCATGA CGGATGACAA AGTGGACAAG 
ACCCTGGAGC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTG 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TTCCTGTCAA GAAAGCGGCC 
CAACAGCGGA GCTTCCATTT CCAGTCGTTT CGAGAGAATC GTCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTACGAAA GACAATGAAG 
TACGAGGACA CACAGCACAT CCTCTGTCAC CTGAATATCA CCATGCCCCC CTGCACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACACCCC TGACCCTTCG ATACAGCATT 
CTCAGCGAGT CGCGGCTGGG TTTTACCAGT GACACAGACC GTGTCGAAAT GAGGATGGCT 
GTCATCAGAG AACACCTCGG CCTAAGCTGG GCAGAGCTGG CCCGGGAGCT GCAGTTCAGC 
GTGGAAGACA TCAACCGGAT CCGTGTAGAA AACCCTAACT CCTTGTTGGA CCAGAGTACA 
GCCTTGCTGA CCCTCTGGGT GGACCGTGAA GGCGAAAATG CAAAGATGGA GAATTTGTAC 
ACAGCCCTGC GGAACATCGA CAGGAGTGAG ATTGTTAACA TGTTGGAGGG CTCCGGCAGG 
CAGAGCAGAA ACCTCAAACC AGAGCGGAGA CATGGGGACC GGGAGTACTC ATTGTCACCC 
TCCCAGGTGA ATGGTTACTC CTCGCTGCAG GACGAGCTGC TGTCCCCCGC CTCCCTGCAG 
TACGCTCTCC CCTCTCCACT GTGTGCAGAC CAGTACTGGA ACGAAGTGGC CGTCATAGAC 
GCCATCCCCC TGGCGGCTAC AGAGCATGAC ACCATGCTGG AGATGTCTGA CATGCAGGTG 
TGGTCTGCGG GCCTCACACC CTCCCTGGTC ACTGCTGAGG ACTCCTCTCT GGAGTGCAGC 
AAGGCTGAGG ACTCTGACGC CATACCAGAG TGGAAGTTGG AAGGGGCACA CTCAGAGGAC 
ACGCAGGGCC CGGAGCTGGG CTCTCAGGAC CTGGTGGAGG ACGACACAGT GGATTCAGAT 
GCCACAAATG GCCTGGCAGA TTTGCTAGGT CAGGAGGAAG GTCAGAGGTC AGAAAAGAAG 
AGGCAGGAAG TCTCAGGCAC AGAGCAGGAC ACGGAGACTG AAGTGTCTCT TGTTTCAGGC 
CAGCAGCGAG TTCACGCCCG AATCACAGAC TCACCCTCAG TGAGGCAGGT GCTGGACAGA 
AGCCAGGCCA GAACACTGGA CTGGGATAAA CAGGGTTCCA CAGCGGTACA CCCGCAAGAA 
GCCACACAGA GCTCCTGGCA AGAGGAGGTC ACGCAGGGCC CACACTCATT CCAGAGAAGG 
ATCACCACCA TCCAAGGGCC GGAGCCTGGT GCGCTTCAGG AATACGAGCA GGTGCTGGTG 
TCTACCAGGG AGCATGTGCA GAGGGGGCCA CCTGAGACCG GCAGCCCCAA AGCTGGCAAG 
GAACCTAGCC TGTGGGCACC TGAGAGCGCC TTCTCTCAAG AGGTGCAGGG GGATGAGCTT 
CAGAATATTC CAGGAGAGCA GGTGACGGAG GAACAATTCA CAGATGAACA GGGCAACATT 
GTTACCAAGA AGATCATTCG CAAAGTCGTC CGGCAGGTAG ACTCGTCTGG TGCCATCGAC 
ACCCAGCAGC ACGAGGAGGT GATTGTCGAG GGTCCCCTGG CAGACCCTGG GGATCTGGAA 
GCAGATATTG AGTCCTTTAT GAAACTTACT AAGGTGGAGC TAAGAGGGAG TGGACTCCAG 
CCGGACCTGA TAGAGGGCAG GAAGGGGGCT CAGATAGTGA AGCGGGCCAG CCTGAAAAGG 
GGCAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508997.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509060.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X9, mRNA.

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CloneID	OMu40548
Clone ID Related Accession (Same CDS sequence)	XM_006508997.4 , XM_006508997.3
Accession Version	XM_006508997.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG ATCACACCTC AACACCCAAA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509060.1
CDS	350..6055
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508997.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508997.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG TCAGGAGGAA 
GGTCAGAGGT CAGAAAAGAA GAGGCAGGAA GTCTCAGGCA CAGAGCAGGA CACGGAGACT 
GAAGTGTCTC TTGTTTCAGG CCAGCAGCGA GTTCACGCCC GAATCACAGA CTCACCCTCA 
GTGAGGCAGG TGCTGGACAG AAGCCAGGCC AGAACACTGG ACTGGGATAA ACAGGGTTCC 
ACAGCGGTAC ACCCGCAAGA AGCCACACAG AGCTCCTGGC AAGAGGAGGT CACGCAGGGC 
CCACACTCAT TCCAGAGAAG GATCACCACC ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG 
GAATACGAGC AGGTGCTGGT GTCTACCAGG GAGCATGTGC AGAGGGGGCC ACCTGAGACC 
GGCAGCCCCA AAGCTGGCAA GGAACCTAGC CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA 
GAGGTGCAGG GGGATGAGCT TCAGAATATT CCAGGAGAGC AGGTGACGGA GGAACAATTC 
ACAGATGAAC AGGGCAACAT TGTTACCAAG AAGATCATTC GCAAAGTCGT CCGGCAGGTA 
GACTCGTCTG GTGCCATCGA CACCCAGCAG CACGAGGAGG ATCACACCTC AACACCCAAA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508998.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509061.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X10, mRNA.

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CloneID	OMu40549
Clone ID Related Accession (Same CDS sequence)	XM_006508998.4 , XM_006508998.3
Accession Version	XM_006508998.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508998.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509061.1
CDS	350..6037
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006508998.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508998.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGTTACT CCTCGCTGCA GGACGAGCTG 
CTGTCCCCCG CCTCCCTGCA GTACGCTCTC CCCTCTCCAC TGTGTGCAGA CCAGTACTGG 
AACGAAGTGG CCGTCATAGA CGCCATCCCC CTGGCGGCTA CAGAGCATGA CACCATGCTG 
GAGATGTCTG ACATGCAGGT GTGGTCTGCG GGCCTCACAC CCTCCCTGGT CACTGCTGAG 
GACTCCTCTC TGGAGTGCAG CAAGGCTGAG GACTCTGACG CCATACCAGA GTGGAAGTTG 
GAAGGGGCAC ACTCAGAGGA CACGCAGGGC CCGGAGCTGG GCTCTCAGGA CCTGGTGGAG 
GACGACACAG TGGATTCAGA TGCCACAAAT GGCCTGGCAG ATTTGCTAGG CCAGCAGCGA 
GTTCACGCCC GAATCACAGA CTCACCCTCA GTGAGGCAGG TGCTGGACAG AAGCCAGGCC 
AGAACACTGG ACTGGGATAA ACAGGGTTCC ACAGCGGTAC ACCCGCAAGA AGCCACACAG 
AGCTCCTGGC AAGAGGAGGT CACGCAGGGC CCACACTCAT TCCAGAGAAG GATCACCACC 
ATCCAAGGGC CGGAGCCTGG TGCGCTTCAG GAATACGAGC AGGTGCTGGT GTCTACCAGG 
GAGCATGTGC AGAGGGGGCC ACCTGAGACC GGCAGCCCCA AAGCTGGCAA GGAACCTAGC 
CTGTGGGCAC CTGAGAGCGC CTTCTCTCAA GAGGTGCAGG GGGATGAGCT TCAGAATATT 
CCAGGAGAGC AGGTGACGGA GGAACAATTC ACAGATGAAC AGGGCAACAT TGTTACCAAG 
AAGATCATTC GCAAAGTCGT CCGGCAGGTA GACTCGTCTG GTGCCATCGA CACCCAGCAG 
CACGAGGAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006508999.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509062.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X11, mRNA.

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CloneID	OMu40550
Clone ID Related Accession (Same CDS sequence)	XM_006508999.4 , XM_006508999.3
Accession Version	XM_006508999.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGATCA CACCTCAACA CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509062.1
CDS	350..6031
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEDHTSTPKP

Target ORF information:

RefSeq Version	XM_006508999.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508999.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC 
GCTCTCCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCTACAGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTGCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCTGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAAG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGTCAG GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG 
CAGGAAGTCT CAGGCACAGA GCAGGACACG GAGACTGAAG TGTCTCTTGT TTCAGGCCAG 
CAGCGAGTTC ACGCCCGAAT CACAGACTCA CCCTCAGTGA GGCAGGTGCT GGACAGAAGC 
CAGGCCAGAA CACTGGACTG GGATAAACAG GGTTCCACAG CGGTACACCC GCAAGAAGCC 
ACACAGAGCT CCTGGCAAGA GGAGGTCACG CAGGGCCCAC ACTCATTCCA GAGAAGGATC 
ACCACCATCC AAGGGCCGGA GCCTGGTGCG CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT 
ACCAGGGAGC ATGTGCAGAG GGGGCCACCT GAGACCGGCA GCCCCAAAGC TGGCAAGGAA 
CCTAGCCTGT GGGCACCTGA GAGCGCCTTC TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG 
AATATTCCAG GAGAGCAGGT GACGGAGGAA CAATTCACAG ATGAACAGGG CAACATTGTT 
ACCAAGAAGA TCATTCGCAA AGTCGTCCGG CAGGTAGACT CGTCTGGTGC CATCGACACC 
CAGCAGCACG AGGAGGATCA CACCTCAACA CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509000.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509063.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X12, mRNA.

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CloneID	OMu40551
Clone ID Related Accession (Same CDS sequence)	XM_006509000.2
Accession Version	XM_006509000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GAACTGCTCA CATATCTATA 
ATGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAGTC TCCAGCCATC 
CCAAGGATCC CTTGTGTCAC ACCTGAGACC GTTGTGATCC GATCTGAAGA TCAGGAACAA 
GCATCTAAAG AATATGATGA GGATTCCCTC ATTCCCAGCA GCCCGGCCAC AGAGACCTCA 
GACAACATTA GCCCTGTGGC TAGCCCGGTC CACACAGGGT TCCTGGTGAG CTTCATGGTG 
GATGCCCGAG GGGGATCCAT GAGAGGAAGC CGTCATAATG GCCTCAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG 
AACACGCCAC CCCCACTGGC TGAGGAAGAG GGCCTAGCCA GCAGGATCAT TGCCCTGGGA 
CCCACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCATTT TGCCTCCCAT 
GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG 
CACAAGAGTC GTTATGGGGA AAGCTACCTG GACCAGATCC TTAATGGCAT GGATGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA CGTGTGTGCC GCATCATCAC CACCGACTTC 
CCTCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGTTCCC TGAGAAGCAA GCTGGTACCG CTGGTACAGG CAACGTTCCC TGAAAACGCT 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCGGATGA GCTGGTGACC 
AAGCTCCTGG GTAACCAGGC CACATTCAGC CCCATTGTGA CCGTGGAGCC GAGGCGTCGG 
AAGTTCCACC GTCCCATCGG GCTGCGAATC CCGCTCCCTC CCTCATGGAC GGACAACCCC 
AGGGACAGTG GGGAGGGAGA CACCACCAGC CTGCGCCTAC TGTGTAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AACTTATATA CGCCAATGAG 
TGTGCCAACT TTACCACCAA TGTCTCAGCC AGGTTCTGGC TGTCAGACTG TCCCCGGACT 
GCGGAGGCTG TGCACTTTGC CACTCTCCTG TACAAAGAGC TCACAGCAGT CCCCTACATG 
GCTAAGTTTG TCATTTTTGC CAAGATGAAC GACGCTCGGG AAGGACGGCT CCGTTGCTAC 
TGCATGACGG ATGACAAAGT GGACAAGACC CTGGAGCAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTGGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTTC CTGTCAAGAA AGCGGCCCAA CAGCGGAGCT TCCATTTCCA GTCGTTTCGA 
GAGAATCGTC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TACGAAAGAC AATGAAGTAC GAGGACACAC AGCACATCCT CTGTCACCTG 
AATATCACCA TGCCCCCCTG CACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACACCCCTGA CCCTTCGATA CAGCATTCTC AGCGAGTCGC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TCGAAATGAG GATGGCTGTC ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA 
GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG GAAGACATCA ACCGGATCCG TGTAGAAAAC 
CCTAACTCCT TGTTGGACCA GAGTACAGCC TTGCTGACCC TCTGGGTGGA CCGTGAAGGC 
GAAAATGCAA AGATGGAGAA TTTGTACACA GCCCTGCGGA ACATCGACAG GAGTGAGATT 
GTTAACATGT TGGAGGGCTC CGGCAGGCAG AGCAGAAACC TCAAACCAGA GCGGAGACAT 
GGGGACCGGG AGTACTCATT GTCACCCTCC CAGGTGAATG GTTACTCCTC GCTGCAGGAC 
GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC GCTCTCCCCT CTCCACTGTG TGCAGACCAG 
TACTGGAACG AAGTGGCCGT CATAGACGCC ATCCCCCTGG CGGCTACAGA GCATGACACC 
ATGCTGGAGA TGTCTGACAT GCAGGTGTGG TCTGCGGGCC TCACACCCTC CCTGGTCACT 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CTGACGCCAT ACCAGAGTGG 
AAGTTGGAAG GGGCACACTC AGAGGACACG CAGGGCCCGG AGCTGGGCTC TCAGGACCTG 
GTGGAGGACG ACACAGTGGA TTCAGATGCC ACAAATGGCC TGGCAGATTT GCTAGGTCAG 
GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG CAGGAAGTCT CAGGCACAGA GCAGGACACG 
GAGACTGAAG TGTCTCTTGT TTCAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CACTGGACTG GGATAAACAG 
GGTTCCACAG CGGTACACCC GCAAGAAGCC ACACAGAGCT CCTGGCAAGA GGAGGTCACG 
CAGGGCCCAC ACTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCTGGTGCG 
CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGACCGGCA GCCCCAAAGC TGGCAAGGAA CCTAGCCTGT GGGCACCTGA GAGCGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACGGAGGAA 
CAATTCACAG ATGAACAGGG CAACATTGTT ACCAAGAAGA TCATTCGCAA AGTCGTCCGG 
CAGGTAGACT CGTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGATCA CACCTCAACA 
CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509063.1
CDS	301..5952
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEDHTSTPKP

Target ORF information:

RefSeq Version	XM_006509000.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509000.2

ORF Insert Sequence:

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTCTG CGGGCGGCAC GCTCGGGGAA CCTGGACAAG 
GCTCTGGATC ACCTGCGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAC 
GGCCTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACGAC AACCAAGAAG GGGAACACTG CTCTGCACAT CGCTGCCCTT 
GCTGGTCAGG ATGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCTCAGAAAG GCTTTACTCC CCTGTACATG GCTGCTCAGG AGAACCACTT GGAAGTGGTG 
AAATTTCTAC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAAGATGG CTTCACCCCA 
CTGGCCGTGG CTCTACAGCA GGGTCACGAG AATGTGGTGG CTCACCTCAT CAACTATGGG 
ACGAAAGGGA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC TCACTATGAG AACCTCAACG TGGCCCAGTT GCTCCTCAAC 
AGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGCATCA CCCCACTACA CATCGCCTCC 
CGCAGGGGGA ACGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAATTGAC ACCGCTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCAATCCACA TGGCCGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AATGCAGAGA TAGACGACAT CACCTTGGAT CACCTGACTC CTCTCCATGT GGCAGCCCAC 
TGTGGCCACC ACCGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC CAACTCCAGA 
GCCCTGAATG GTTTTACCCC GTTACACATC GCCTGCAAGA AGAACCACAT CCGTGTAATG 
GAGTTGCTGC TGAAGACAGG AGCCTCCATC GACGCGGTCA CTGAGTCTGG CCTGACACCT 
CTCCACGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTACT GCAGCGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCCT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA CGCCAAGGCC 
AAGGATGACC AGACACCGCT TCACTGTGCT GCTCGAATCG GCCACACAGG CATGGTGAAG 
CTCCTGCTGG AGAATGGTGC CAGCCCCAAT CTGGCTACCA CTGCTGGCCA CACACCCCTA 
CACACCGCAG CCCGTGAGGG ACACGTGGAC ACAGCCCTGG CCCTGCTGGA GAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTGC ACGTGGCTGC TAAGTACGGG 
AAGGTACGGT TGGCGGAGCT GCTGCTGGAA CACGATGCAC ACCCCAATGC AGCTGGGAAG 
AACGGCTTGA CTCCTCTGCA TGTGGCCGTC CATCACAACA ACCTGGACAT TGTCAAACTT 
CTTCTTCCCC GAGGTGGCTC CCCCCACAGC CCTGCCTGGA ATGGCTACAC TCCTTTGCAC 
ATCGCTGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAGTA CGGAGGGTCA 
GCGAATGCAG AGTCGGTACA AGGCGTGACC CCACTTCACC TGGCTGCCCA AGAGGGCCAC 
ACAGAAATGG TTGCTCTTCT CCTGTCCAAG CAAGCCAACG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CCCTCCACCT GGTGTCACAA GAAGGCCATG TTCCAGTGGC AGACGTGCTG 
ATCAAACATG GCGTCACCGT GGATGCTACC ACCCGGATGG GTTACACCCC GCTCCATGTG 
GCCAGTCATT ATGGAAACAT CAAGCTGGTG AAGTTCTTGC TACAGCACCA GGCAGATGTC 
AATGCCAAGA CCAAGCTAGG ATACAGCCCC TTGCACCAGG CAGCTCAACA AGGACACACA 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACGGTGG ACGAGATCCT GGACGTGTCT GAAGATGAAG GAACTGCTCA CATATCTATA 
ATGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAGTC TCCAGCCATC 
CCAAGGATCC CTTGTGTCAC ACCTGAGACC GTTGTGATCC GATCTGAAGA TCAGGAACAA 
GCATCTAAAG AATATGATGA GGATTCCCTC ATTCCCAGCA GCCCGGCCAC AGAGACCTCA 
GACAACATTA GCCCTGTGGC TAGCCCGGTC CACACAGGGT TCCTGGTGAG CTTCATGGTG 
GATGCCCGAG GGGGATCCAT GAGAGGAAGC CGTCATAATG GCCTCAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG 
AACACGCCAC CCCCACTGGC TGAGGAAGAG GGCCTAGCCA GCAGGATCAT TGCCCTGGGA 
CCCACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCATTT TGCCTCCCAT 
GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG 
CACAAGAGTC GTTATGGGGA AAGCTACCTG GACCAGATCC TTAATGGCAT GGATGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA CGTGTGTGCC GCATCATCAC CACCGACTTC 
CCTCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGTTCCC TGAGAAGCAA GCTGGTACCG CTGGTACAGG CAACGTTCCC TGAAAACGCT 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCGGATGA GCTGGTGACC 
AAGCTCCTGG GTAACCAGGC CACATTCAGC CCCATTGTGA CCGTGGAGCC GAGGCGTCGG 
AAGTTCCACC GTCCCATCGG GCTGCGAATC CCGCTCCCTC CCTCATGGAC GGACAACCCC 
AGGGACAGTG GGGAGGGAGA CACCACCAGC CTGCGCCTAC TGTGTAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AACTTATATA CGCCAATGAG 
TGTGCCAACT TTACCACCAA TGTCTCAGCC AGGTTCTGGC TGTCAGACTG TCCCCGGACT 
GCGGAGGCTG TGCACTTTGC CACTCTCCTG TACAAAGAGC TCACAGCAGT CCCCTACATG 
GCTAAGTTTG TCATTTTTGC CAAGATGAAC GACGCTCGGG AAGGACGGCT CCGTTGCTAC 
TGCATGACGG ATGACAAAGT GGACAAGACC CTGGAGCAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTGGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTTC CTGTCAAGAA AGCGGCCCAA CAGCGGAGCT TCCATTTCCA GTCGTTTCGA 
GAGAATCGTC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TACGAAAGAC AATGAAGTAC GAGGACACAC AGCACATCCT CTGTCACCTG 
AATATCACCA TGCCCCCCTG CACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACACCCCTGA CCCTTCGATA CAGCATTCTC AGCGAGTCGC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TCGAAATGAG GATGGCTGTC ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA 
GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG GAAGACATCA ACCGGATCCG TGTAGAAAAC 
CCTAACTCCT TGTTGGACCA GAGTACAGCC TTGCTGACCC TCTGGGTGGA CCGTGAAGGC 
GAAAATGCAA AGATGGAGAA TTTGTACACA GCCCTGCGGA ACATCGACAG GAGTGAGATT 
GTTAACATGT TGGAGGGCTC CGGCAGGCAG AGCAGAAACC TCAAACCAGA GCGGAGACAT 
GGGGACCGGG AGTACTCATT GTCACCCTCC CAGGTGAATG GTTACTCCTC GCTGCAGGAC 
GAGCTGCTGT CCCCCGCCTC CCTGCAGTAC GCTCTCCCCT CTCCACTGTG TGCAGACCAG 
TACTGGAACG AAGTGGCCGT CATAGACGCC ATCCCCCTGG CGGCTACAGA GCATGACACC 
ATGCTGGAGA TGTCTGACAT GCAGGTGTGG TCTGCGGGCC TCACACCCTC CCTGGTCACT 
GCTGAGGACT CCTCTCTGGA GTGCAGCAAG GCTGAGGACT CTGACGCCAT ACCAGAGTGG 
AAGTTGGAAG GGGCACACTC AGAGGACACG CAGGGCCCGG AGCTGGGCTC TCAGGACCTG 
GTGGAGGACG ACACAGTGGA TTCAGATGCC ACAAATGGCC TGGCAGATTT GCTAGGTCAG 
GAGGAAGGTC AGAGGTCAGA AAAGAAGAGG CAGGAAGTCT CAGGCACAGA GCAGGACACG 
GAGACTGAAG TGTCTCTTGT TTCAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CACTGGACTG GGATAAACAG 
GGTTCCACAG CGGTACACCC GCAAGAAGCC ACACAGAGCT CCTGGCAAGA GGAGGTCACG 
CAGGGCCCAC ACTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCTGGTGCG 
CTTCAGGAAT ACGAGCAGGT GCTGGTGTCT ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGACCGGCA GCCCCAAAGC TGGCAAGGAA CCTAGCCTGT GGGCACCTGA GAGCGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACGGAGGAA 
CAATTCACAG ATGAACAGGG CAACATTGTT ACCAAGAAGA TCATTCGCAA AGTCGTCCGG 
CAGGTAGACT CGTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGATCA CACCTCAACA 
CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509001.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509064.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X13, mRNA.

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CloneID	OMu40552
Clone ID Related Accession (Same CDS sequence)	XM_006509001.3 , XM_006509001.4
Accession Version	XM_006509001.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGCCAGC AGCGAGTTCA CGCCCGAATC 
ACAGACTCAC CCTCAGTGAG GCAGGTGCTG GACAGAAGCC AGGCCAGAAC ACTGGACTGG 
GATAAACAGG GTTCCACAGC GGTACACCCG CAAGAAGCCA CACAGAGCTC CTGGCAAGAG 
GAGGTCACGC AGGGCCCACA CTCATTCCAG AGAAGGATCA CCACCATCCA AGGGCCGGAG 
CCTGGTGCGC TTCAGGAATA CGAGCAGGTG CTGGTGTCTA CCAGGGAGCA TGTGCAGAGG 
GGGCCACCTG AGACCGGCAG CCCCAAAGCT GGCAAGGAAC CTAGCCTGTG GGCACCTGAG 
AGCGCCTTCT CTCAAGAGGT GCAGGGGGAT GAGCTTCAGA ATATTCCAGG AGAGCAGGTG 
ACGGAGGAAC AATTCACAGA TGAACAGGGC AACATTGTTA CCAAGAAGAT CATTCGCAAA 
GTCGTCCGGC AGGTAGACTC GTCTGGTGCC ATCGACACCC AGCAGCACGA GGAGGTGATT 
GTCGAGGGTC CCCTGGCAGA CCCTGGGGAT CTGGAAGCAG ATATTGAGTC CTTTATGAAA 
CTTACTAAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509064.1
CDS	350..5737
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	XM_006509001.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509001.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGAACTG CTCACATATC TATAATGGGT 
GACGAACTTG TCGGCTCGAA GGCTGAAAGG CGGGACTCCC GGGATGTGGG TGAGGAGAAA 
GAGCTGTTGG ATTTTGTGCC GAAGCTAGAC CAAGTGGTGG AGTCTCCAGC CATCCCAAGG 
ATCCCTTGTG TCACACCTGA GACCGTTGTG ATCCGATCTG AAGATCAGGA ACAAGCATCT 
AAAGAATATG ATGAGGATTC CCTCATTCCC AGCAGCCCGG CCACAGAGAC CTCAGACAAC 
ATTAGCCCTG TGGCTAGCCC GGTCCACACA GGGTTCCTGG TGAGCTTCAT GGTGGATGCC 
CGAGGGGGAT CCATGAGAGG AAGCCGTCAT AATGGCCTCA GGGTGGTGAT CCCTCCCCGG 
ACATGCGCAG CACCCACTCG CATCACCTGC CGCCTCGTTA AGCCGCAGAA GCTGAACACG 
CCACCCCCAC TGGCTGAGGA AGAGGGCCTA GCCAGCAGGA TCATTGCCCT GGGACCCACG 
GGGGCCCAGT TCCTTAGCCC TGTGATCGTG GAAATCCCCC ATTTTGCCTC CCATGGCCGT 
GGGGACCGTG AGCTGGTGGT TCTGAGGAGT GAAAACGGCT CTGTGTGGAA GGAGCACAAG 
AGTCGTTATG GGGAAAGCTA CCTGGACCAG ATCCTTAATG GCATGGATGA AGAGCTGGGG 
AGCCTGGAGG AGCTGGAGAA GAAACGTGTG TGCCGCATCA TCACCACCGA CTTCCCTCTG 
TACTTTGTCA TCATGTCCCG GCTCTGCCAG GACTACGACA CCATCGGTCC TGAAGGGGGT 
TCCCTGAGAA GCAAGCTGGT ACCGCTGGTA CAGGCAACGT TCCCTGAAAA CGCTGTGACC 
AAGAAAGTGA AGCTGGCTCT GCAGGCCCAG CCTGTCCCGG ATGAGCTGGT GACCAAGCTC 
CTGGGTAACC AGGCCACATT CAGCCCCATT GTGACCGTGG AGCCGAGGCG TCGGAAGTTC 
CACCGTCCCA TCGGGCTGCG AATCCCGCTC CCTCCCTCAT GGACGGACAA CCCCAGGGAC 
AGTGGGGAGG GAGACACCAC CAGCCTGCGC CTACTGTGTA GCGTCATCGG TGGAACTGAC 
CAAGCCCAGT GGGAAGATAT AACGGGAACA ACCAAACTTA TATACGCCAA TGAGTGTGCC 
AACTTTACCA CCAATGTCTC AGCCAGGTTC TGGCTGTCAG ACTGTCCCCG GACTGCGGAG 
GCTGTGCACT TTGCCACTCT CCTGTACAAA GAGCTCACAG CAGTCCCCTA CATGGCTAAG 
TTTGTCATTT TTGCCAAGAT GAACGACGCT CGGGAAGGAC GGCTCCGTTG CTACTGCATG 
ACGGATGACA AAGTGGACAA GACCCTGGAG CAGCATGAAA ACTTCGTGGA GGTGGCCCGG 
AGCAGGGACA TAGAGGTTCT GGAAGGGATG CCTCTGTTTG CAGAACTCTC TGGGAACTTG 
GTTCCTGTCA AGAAAGCGGC CCAACAGCGG AGCTTCCATT TCCAGTCGTT TCGAGAGAAT 
CGTCTGGCCA TCCCCGTGAA GGTGAGGGAC AGCAGCCGCG AGCCAGGAGG GTTCTTGTCG 
TTCCTACGAA AGACAATGAA GTACGAGGAC ACACAGCACA TCCTCTGTCA CCTGAATATC 
ACCATGCCCC CCTGCACCAA GGGCAGTGGA GCAGAAGACA GGAGAAGGAC CCTGACACCC 
CTGACCCTTC GATACAGCAT TCTCAGCGAG TCGCGGCTGG GTTTTACCAG TGACACAGAC 
CGTGTCGAAA TGAGGATGGC TGTCATCAGA GAACACCTCG GCCTAAGCTG GGCAGAGCTG 
GCCCGGGAGC TGCAGTTCAG CGTGGAAGAC ATCAACCGGA TCCGTGTAGA AAACCCTAAC 
TCCTTGTTGG ACCAGAGTAC AGCCTTGCTG ACCCTCTGGG TGGACCGTGA AGGCGAAAAT 
GCAAAGATGG AGAATTTGTA CACAGCCCTG CGGAACATCG ACAGGAGTGA GATTGTTAAC 
ATGTTGGAGG GCTCCGGCAG GCAGAGCAGA AACCTCAAAC CAGAGCGGAG ACATGGGGAC 
CGGGAGTACT CATTGTCACC CTCCCAGGTG AATGGCCAGC AGCGAGTTCA CGCCCGAATC 
ACAGACTCAC CCTCAGTGAG GCAGGTGCTG GACAGAAGCC AGGCCAGAAC ACTGGACTGG 
GATAAACAGG GTTCCACAGC GGTACACCCG CAAGAAGCCA CACAGAGCTC CTGGCAAGAG 
GAGGTCACGC AGGGCCCACA CTCATTCCAG AGAAGGATCA CCACCATCCA AGGGCCGGAG 
CCTGGTGCGC TTCAGGAATA CGAGCAGGTG CTGGTGTCTA CCAGGGAGCA TGTGCAGAGG 
GGGCCACCTG AGACCGGCAG CCCCAAAGCT GGCAAGGAAC CTAGCCTGTG GGCACCTGAG 
AGCGCCTTCT CTCAAGAGGT GCAGGGGGAT GAGCTTCAGA ATATTCCAGG AGAGCAGGTG 
ACGGAGGAAC AATTCACAGA TGAACAGGGC AACATTGTTA CCAAGAAGAT CATTCGCAAA 
GTCGTCCGGC AGGTAGACTC GTCTGGTGCC ATCGACACCC AGCAGCACGA GGAGGTGATT 
GTCGAGGGTC CCCTGGCAGA CCCTGGGGAT CTGGAAGCAG ATATTGAGTC CTTTATGAAA 
CTTACTAAGG TGGAGCTAAG AGGGAGTGGA CTCCAGCCGG ACCTGATAGA GGGCAGGAAG 
GGGGCTCAGA TAGTGAAGCG GGCCAGCCTG AAAAGGGGCA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_006509002.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509065.1 Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X14, mRNA.

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CloneID	OMu40553
Clone ID Related Accession (Same CDS sequence)	XM_006509002.3 , XM_006509002.4
Accession Version	XM_006509002.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ankyrin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509002.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGATTGTCG AGGGTCCCCT GGCAGACCCT 
GGGGATCTGG AAGCAGATAT TGAGTCCTTT ATGAAACTTA CTAAGGATCA CACCTCAACA 
CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509065.1
CDS	350..5641
Translation	MQAPPCTLVPDKAFSGCRVAVASPLGRDCSRCSDSSPSLPPSSPPAGRVLVPSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVIVEGPLADPGDLEADIESFMKLTKDHTSTPKP

Target ORF information:

RefSeq Version	XM_006509002.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ankyrin 1, erythroid (Ank1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509002.3

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTGCCC GATAAGGCCT TTTCTGGTTG CAGGGTAGCA 
GTGGCAAGTC CCCTAGGGAG AGACTGTTCC CGCTGCTCTG ACAGCTCTCC CTCTCTCCCG 
CCCTCCAGCC CTCCGGCCGG CAGGGTCCTG GTCCCGAGCA ACAAGATGGG CTTCTGTAAA 
GCCGATGCTG CTACCAGCTT TCTGCGGGCG GCACGCTCGG GGAACCTGGA CAAGGCTCTG 
GATCACCTGC GCAATGGAGT GGACATTAAC ACCTGTAACC AGAACGGGTT GAACGGCCTG 
CATCTGGCCT CCAAAGAAGG CCATGTGAAG ATGGTGGTTG AACTTCTGCA CAAAGAGATC 
ATTCTAGAAA CGACAACCAA GAAGGGGAAC ACTGCTCTGC ACATCGCTGC CCTTGCTGGT 
CAGGATGAGG TGGTCCGGGA GCTGGTCAAC TATGGAGCCA ATGTCAATGC CCAGTCTCAG 
AAAGGCTTTA CTCCCCTGTA CATGGCTGCT CAGGAGAACC ACTTGGAAGT GGTGAAATTT 
CTACTGGAGA ATGGAGCCAA TCAGAATGTA GCCACAGAAG ATGGCTTCAC CCCACTGGCC 
GTGGCTCTAC AGCAGGGTCA CGAGAATGTG GTGGCTCACC TCATCAACTA TGGGACGAAA 
GGGAAAGTGC GCCTCCCTGC CCTGCACATC GCGGCCCGCA ACGATGACAC ACGGACAGCC 
GCAGTCCTTC TGCAGAATGA CCCCAACCCA GATGTGCTTT CCAAGACGGG ATTCACACCC 
CTCCACATCG CAGCTCACTA TGAGAACCTC AACGTGGCCC AGTTGCTCCT CAACAGGGGA 
GCCAGCGTCA ACTTCACACC TCAGAATGGC ATCACCCCAC TACACATCGC CTCCCGCAGG 
GGGAACGTGA TCATGGTGAG ACTCCTGCTG GACCGAGGGG CTCAGATAGA AACGAGGACC 
AAGGATGAAT TGACACCGCT CCACTGTGCA GCTCGGAATG GACACGTGAG AATCTCAGAG 
ATCCTGCTGG ACCACGGGGC ACCCATCCAA GCCAAAACCA AGAATGGCTT GTCCCCAATC 
CACATGGCCG CTCAGGGAGA CCACCTCGAC TGTGTCCGAC TTCTATTGCA ATACAATGCA 
GAGATAGACG ACATCACCTT GGATCACCTG ACTCCTCTCC ATGTGGCAGC CCACTGTGGC 
CACCACCGGG TGGCTAAGGT TCTTTTGGAT AAAGGGGCCA AGCCCAACTC CAGAGCCCTG 
AATGGTTTTA CCCCGTTACA CATCGCCTGC AAGAAGAACC ACATCCGTGT AATGGAGTTG 
CTGCTGAAGA CAGGAGCCTC CATCGACGCG GTCACTGAGT CTGGCCTGAC ACCTCTCCAC 
GTAGCCTCCT TCATGGGACA CCTTCCTATT GTGAAGAACT TACTGCAGCG GGGAGCGTCA 
CCCAATGTCT CCAATGTGAA AGTAGAAACC CCCTTGCACA TGGCAGCCCG AGCAGGGCAT 
ACAGAAGTGG CCAAATATTT GCTCCAGAAC AAAGCCAAAG CCAACGCCAA GGCCAAGGAT 
GACCAGACAC CGCTTCACTG TGCTGCTCGA ATCGGCCACA CAGGCATGGT GAAGCTCCTG 
CTGGAGAATG GTGCCAGCCC CAATCTGGCT ACCACTGCTG GCCACACACC CCTACACACC 
GCAGCCCGTG AGGGACACGT GGACACAGCC CTGGCCCTGC TGGAGAAGGA GGCATCCCAA 
GCCTGCATGA CCAAGAAAGG ATTTACCCCT CTGCACGTGG CTGCTAAGTA CGGGAAGGTA 
CGGTTGGCGG AGCTGCTGCT GGAACACGAT GCACACCCCA ATGCAGCTGG GAAGAACGGC 
TTGACTCCTC TGCATGTGGC CGTCCATCAC AACAACCTGG ACATTGTCAA ACTTCTTCTT 
CCCCGAGGTG GCTCCCCCCA CAGCCCTGCC TGGAATGGCT ACACTCCTTT GCACATCGCT 
GCCAAGCAGA ACCAGATAGA GGTGGCCCGC AGTCTACTGC AGTACGGAGG GTCAGCGAAT 
GCAGAGTCGG TACAAGGCGT GACCCCACTT CACCTGGCTG CCCAAGAGGG CCACACAGAA 
ATGGTTGCTC TTCTCCTGTC CAAGCAAGCC AACGGCAACC TGGGGAACAA GAGTGGACTC 
ACTCCCCTCC ACCTGGTGTC ACAAGAAGGC CATGTTCCAG TGGCAGACGT GCTGATCAAA 
CATGGCGTCA CCGTGGATGC TACCACCCGG ATGGGTTACA CCCCGCTCCA TGTGGCCAGT 
CATTATGGAA ACATCAAGCT GGTGAAGTTC TTGCTACAGC ACCAGGCAGA TGTCAATGCC 
AAGACCAAGC TAGGATACAG CCCCTTGCAC CAGGCAGCTC AACAAGGACA CACAGACATT 
GTGACATTGC TCCTGAAGAA TGGTGCTTCT CCAAATGAGG TCAGCTCGAA CGGAACCACA 
CCTCTGGCAA TAGCCAAACG TTTGGGCTAC ATCTCTGTAA CAGACGTGCT CAAGGTGGTC 
ACAGATGAAA CCAGTGTGGT GTTAGTCAGT GACAAACACC GGATGAGCTA CCCTGAGACG 
GTGGACGAGA TCCTGGACGT GTCTGAAGAT GAAGGTGACG AACTTGTCGG CTCGAAGGCT 
GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG GAGAAAGAGC TGTTGGATTT TGTGCCGAAG 
CTAGACCAAG TGGTGGAGTC TCCAGCCATC CCAAGGATCC CTTGTGTCAC ACCTGAGACC 
GTTGTGATCC GATCTGAAGA TCAGGAACAA GCATCTAAAG AATATGATGA GGATTCCCTC 
ATTCCCAGCA GCCCGGCCAC AGAGACCTCA GACAACATTA GCCCTGTGGC TAGCCCGGTC 
CACACAGGGT TCCTGGTGAG CTTCATGGTG GATGCCCGAG GGGGATCCAT GAGAGGAAGC 
CGTCATAATG GCCTCAGGGT GGTGATCCCT CCCCGGACAT GCGCAGCACC CACTCGCATC 
ACCTGCCGCC TCGTTAAGCC GCAGAAGCTG AACACGCCAC CCCCACTGGC TGAGGAAGAG 
GGCCTAGCCA GCAGGATCAT TGCCCTGGGA CCCACGGGGG CCCAGTTCCT TAGCCCTGTG 
ATCGTGGAAA TCCCCCATTT TGCCTCCCAT GGCCGTGGGG ACCGTGAGCT GGTGGTTCTG 
AGGAGTGAAA ACGGCTCTGT GTGGAAGGAG CACAAGAGTC GTTATGGGGA AAGCTACCTG 
GACCAGATCC TTAATGGCAT GGATGAAGAG CTGGGGAGCC TGGAGGAGCT GGAGAAGAAA 
CGTGTGTGCC GCATCATCAC CACCGACTTC CCTCTGTACT TTGTCATCAT GTCCCGGCTC 
TGCCAGGACT ACGACACCAT CGGTCCTGAA GGGGGTTCCC TGAGAAGCAA GCTGGTACCG 
CTGGTACAGG CAACGTTCCC TGAAAACGCT GTGACCAAGA AAGTGAAGCT GGCTCTGCAG 
GCCCAGCCTG TCCCGGATGA GCTGGTGACC AAGCTCCTGG GTAACCAGGC CACATTCAGC 
CCCATTGTGA CCGTGGAGCC GAGGCGTCGG AAGTTCCACC GTCCCATCGG GCTGCGAATC 
CCGCTCCCTC CCTCATGGAC GGACAACCCC AGGGACAGTG GGGAGGGAGA CACCACCAGC 
CTGCGCCTAC TGTGTAGCGT CATCGGTGGA ACTGACCAAG CCCAGTGGGA AGATATAACG 
GGAACAACCA AACTTATATA CGCCAATGAG TGTGCCAACT TTACCACCAA TGTCTCAGCC 
AGGTTCTGGC TGTCAGACTG TCCCCGGACT GCGGAGGCTG TGCACTTTGC CACTCTCCTG 
TACAAAGAGC TCACAGCAGT CCCCTACATG GCTAAGTTTG TCATTTTTGC CAAGATGAAC 
GACGCTCGGG AAGGACGGCT CCGTTGCTAC TGCATGACGG ATGACAAAGT GGACAAGACC 
CTGGAGCAGC ATGAAAACTT CGTGGAGGTG GCCCGGAGCA GGGACATAGA GGTTCTGGAA 
GGGATGCCTC TGTTTGCAGA ACTCTCTGGG AACTTGGTTC CTGTCAAGAA AGCGGCCCAA 
CAGCGGAGCT TCCATTTCCA GTCGTTTCGA GAGAATCGTC TGGCCATCCC CGTGAAGGTG 
AGGGACAGCA GCCGCGAGCC AGGAGGGTTC TTGTCGTTCC TACGAAAGAC AATGAAGTAC 
GAGGACACAC AGCACATCCT CTGTCACCTG AATATCACCA TGCCCCCCTG CACCAAGGGC 
AGTGGAGCAG AAGACAGGAG AAGGACCCTG ACACCCCTGA CCCTTCGATA CAGCATTCTC 
AGCGAGTCGC GGCTGGGTTT TACCAGTGAC ACAGACCGTG TCGAAATGAG GATGGCTGTC 
ATCAGAGAAC ACCTCGGCCT AAGCTGGGCA GAGCTGGCCC GGGAGCTGCA GTTCAGCGTG 
GAAGACATCA ACCGGATCCG TGTAGAAAAC CCTAACTCCT TGTTGGACCA GAGTACAGCC 
TTGCTGACCC TCTGGGTGGA CCGTGAAGGC GAAAATGCAA AGATGGAGAA TTTGTACACA 
GCCCTGCGGA ACATCGACAG GAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA GCGGAGACAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GCCAGCAGCG AGTTCACGCC CGAATCACAG ACTCACCCTC AGTGAGGCAG 
GTGCTGGACA GAAGCCAGGC CAGAACACTG GACTGGGATA AACAGGGTTC CACAGCGGTA 
CACCCGCAAG AAGCCACACA GAGCTCCTGG CAAGAGGAGG TCACGCAGGG CCCACACTCA 
TTCCAGAGAA GGATCACCAC CATCCAAGGG CCGGAGCCTG GTGCGCTTCA GGAATACGAG 
CAGGTGCTGG TGTCTACCAG GGAGCATGTG CAGAGGGGGC CACCTGAGAC CGGCAGCCCC 
AAAGCTGGCA AGGAACCTAG CCTGTGGGCA CCTGAGAGCG CCTTCTCTCA AGAGGTGCAG 
GGGGATGAGC TTCAGAATAT TCCAGGAGAG CAGGTGACGG AGGAACAATT CACAGATGAA 
CAGGGCAACA TTGTTACCAA GAAGATCATT CGCAAAGTCG TCCGGCAGGT AGACTCGTCT 
GGTGCCATCG ACACCCAGCA GCACGAGGAG GTGATTGTCG AGGGTCCCCT GGCAGACCCT 
GGGGATCTGG AAGCAGATAT TGAGTCCTTT ATGAAACTTA CTAAGGATCA CACCTCAACA 
CCCAAACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria.
G3 (Bethesda, Md.)7(9)3133-3144(2017 Sep)
Huang HM,Bauer DC,Lelliott PM,Dixon MWA,Tilley L,McMorran BJ,Foote SJ,Burgio G

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Scientific reports7(1)5164(2017 Jul)
Huang YS,Delgadillo LF,Cyr KH,Kingsley PD,An X,McGrath KE,Mohandas N,Conboy JG,Waugh RE,Wan J,Palis J

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Scientific reports637197(2016 Nov)
Huang HM,Bauer DC,Lelliott PM,Greth A,McMorran BJ,Foote SJ,Burgio G

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Ank1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

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