Ferd3l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ferd3l
Entrez Gene ID	114712
Full Name	Fer3 like bHLH transcription factor
Synonyms	Mnato3,N-twist,Nato,Nato3,Ntwist,Ptfb,bHLHa31,fer3
General protein information	Preferred Names Fer3 like bHLH transcription factor Names fer3-like protein Fer3-like basic helix-loop-helix protein N-twist nephew of atonal 3 neuronal twist pancreas-specific transcription factor b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_033522.2	NP_277057.1	fer3-like protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	FERD3L	NP_690862.1
Canis lupus familiaris (dog)	FERD3L	XP_539457.2
Rattus norvegicus (Norway rat)	Ferd3l	NP_001102450.1
Bos taurus (cattle)	FERD3L	XP_002686749.1
Pan troglodytes (chimpanzee)	FERD3L	XP_527676.1
	FERD3L	XP_001103690.1
Mus musculus (house mouse)	Ferd3l	NP_277057.1
Gallus gallus (chicken)	FERD3L	XP_425989.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide characterisation of Foxa1 binding sites reveals several mechanisms for regulating neuronal differentiation in midbrain dopamine cells.
Metzakopian E, Bouhali K, Alvarez-Saavedra M, Whitsett JA, Picketts DJ, Ang SL
Development (Cambridge, England)142(7)1315-24(2015 Apr)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Nato3 plays an integral role in dorsoventral patterning of the spinal cord by segregating floor plate/p3 fates via Nkx2.2 suppression and Foxa2 maintenance.
Mansour AA, Khazanov-Zisman S, Netser Y, Klar A, Ben-Arie N
Development (Cambridge, England)141(3)574-84(2014 Feb)

Nato3 integrates with the Shh-Foxa2 transcriptional network regulating the differentiation of midbrain dopaminergic neurons.
Nissim-Eliraz E, Zisman S, Schatz O, Ben-Arie N
Journal of molecular neuroscience : MN51(1)13-27(2013 Sep)

Classification and evolutionary analysis of the basic helix-loop-helix gene family in the green anole lizard, Anolis carolinensis.
Liu A, Wang Y, Zhang D, Wang X, Song H, Dang C, Yao Q, Chen K
Molecular genetics and genomics : MGG288(7-8)365-80(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nato3 induces ectopic Foxa2-positive cells and indirectly downregulates Nkx2.2 expression.
Title: Nato3 plays an integral role in dorsoventral patterning of the spinal cord by segregating floor plate/p3 fates via Nkx2.2 suppression and Foxa2 maintenance.

Nato3 transcription is directly regulated by Foxa2 and increases in parallel to differentiation of dopaminergic cells.
Title: Nato3 integrates with the Shh-Foxa2 transcriptional network regulating the differentiation of midbrain dopaminergic neurons.

Data suggest that Nato3 is a direct target of Foxa2, a transcription activator and effector of Sonic hedgehog, the hallmark regulator of neural tube floor plate induction and spinal cord development.
Title: Foxa2 regulates the expression of Nato3 in the floor plate by a novel evolutionarily conserved promoter.

in the developing mesencephalon, loss of Nato3 did not affect floor plate (FP) differentiation, but led to loss of neurogenic activity in the medial subpopulation of mesFP cells by suppressing proneural gene expression and inducing cell cycle arrest
Title: The basic helix-loop-helix transcription factor Nato3 controls neurogenic activity in mesencephalic floor plate cells.

The following Ferd3l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ferd3l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14780	NM_033522.2 Latest version!	Mus musculus Fer3 like bHLH transcription factor (Ferd3l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ferd3l ( NM_033522.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_277057.1 Mus musculus Fer3 like bHLH transcription factor (Ferd3l), mRNA.

Price & Availability↑

CloneID	OMu14780
Clone ID Related Accession (Same CDS sequence)	NM_033522.2
Accession Version	NM_033522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	fer3-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF517121.1. On Jun 19, 2003 this sequence version replaced NM_033522.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AF517121.1 [ECO:0000345] ##Evidence-Data-END##

ATGGCCGCCT ATCCAGAGAG CTGCTTGGAT GCTACCGTGC TGAACTTCGT AGCAGATCTC 
TCTCTGGCCT CTCCCAGACA CCCTCTTCTC TGCGAGTTCC CACCTGGGGT CCCTTTTGGG 
GACCGAACAC TGGGGTACAG AGAGGGAAGA CCTGGGAGAC TGTCGCAGTT TGATGAAAGA 
TATCAGGAAG TAGAGGGGGA CGAAGTGGAA TATGAGGACC CAGAAGAGGA GGAAGAGGAG 
GGAGAGGGGC GCGGCAGAGT AGCATCCTTG CTGGGCCGCC CCAAAAGAAA AAGAGTTATT 
ACTTATGCCC AGCGCCAGGC CGCCAACATT CGCGAGAGGA AGAGGATGTT CAACCTAAAC 
GAGGCCTTCG ACCAGCTGCG CAGAAAGGTA CCCACCTTCG CTTATGAGAA GAGACTGTCG 
AGGATCGAGA CCCTCCGCTT GGCCATCGTC TACATTTCCT TCATGACCGA GCTCCTGCAG 
AGCAAGGAGG AAAAGGAGGC CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277057.1
CDS	66..572
Translation	MAAYPESCLDATVLNFVADLSLASPRHPLLCEFPPGVPFGDRTLGYREGRPGRLSQFDERYQEVEGDEVEYEDPEEEEEEGEGRGRVASLLGRPKRKRVITYAQRQAANIRERKRMFNLNEAFDQLRRKVPTFAYEKRLSRIETLRLAIVYISFMTELLQSKEEKEAS

Target ORF information:

RefSeq Version	NM_033522.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fer3 like bHLH transcription factor (Ferd3l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033522.2

ORF Insert Sequence:

ATGGCCGCCT ATCCAGAGAG CTGCTTGGAT GCTACCGTGC TGAACTTCGT AGCAGATCTC 
TCTCTGGCCT CTCCCAGACA CCCTCTTCTC TGCGAGTTCC CACCTGGGGT CCCTTTTGGG 
GACCGAACAC TGGGGTACAG AGAGGGAAGA CCTGGGAGAC TGTCGCAGTT TGATGAAAGA 
TATCAGGAAG TAGAGGGGGA CGAAGTGGAA TATGAGGACC CAGAAGAGGA GGAAGAGGAG 
GGAGAGGGGC GCGGCAGAGT AGCATCCTTG CTGGGCCGCC CCAAAAGAAA AAGAGTTATT 
ACTTATGCCC AGCGCCAGGC CGCCAACATT CGCGAGAGGA AGAGGATGTT CAACCTAAAC 
GAGGCCTTCG ACCAGCTGCG CAGAAAGGTA CCCACCTTCG CTTATGAGAA GAGACTGTCG 
AGGATCGAGA CCCTCCGCTT GGCCATCGTC TACATTTCCT TCATGACCGA GCTCCTGCAG 
AGCAAGGAGG AAAAGGAGGC CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide characterisation of Foxa1 binding sites reveals several mechanisms for regulating neuronal differentiation in midbrain dopamine cells.
Development (Cambridge, England)142(7)1315-24(2015 Apr)
Metzakopian E,Bouhali K,Alvarez-Saavedra M,Whitsett JA,Picketts DJ,Ang SL

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Nato3 plays an integral role in dorsoventral patterning of the spinal cord by segregating floor plate/p3 fates via Nkx2.2 suppression and Foxa2 maintenance.
Development (Cambridge, England)141(3)574-84(2014 Feb)
Mansour AA,Khazanov-Zisman S,Netser Y,Klar A,Ben-Arie N

Nato3 integrates with the Shh-Foxa2 transcriptional network regulating the differentiation of midbrain dopaminergic neurons.
Journal of molecular neuroscience : MN51(1)13-27(2013 Sep)
Nissim-Eliraz E,Zisman S,Schatz O,Ben-Arie N

Classification and evolutionary analysis of the basic helix-loop-helix gene family in the green anole lizard, Anolis carolinensis.
Molecular genetics and genomics : MGG288(7-8)365-80(2013 Aug)
Liu A,Wang Y,Zhang D,Wang X,Song H,Dang C,Yao Q,Chen K