D1Pas1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	D1Pas1
Entrez Gene ID	110957
Full Name	DNA segment, Chr 1, Pasteur Institute 1
Synonyms	Pl10
General protein information	Preferred Names DNA segment, Chr 1, Pasteur Institute 1 Names putative ATP-dependent RNA helicase Pl10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H5\|1 90.09 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_033077.3	NP_149068.1	putative ATP-dependent RNA helicase Pl10

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ddx3	NP_001119895.1
Rattus norvegicus (Norway rat)	RGD1309586	NP_001102328.1
Mus musculus (house mouse)	D1Pas1	NP_149068.1
Danio rerio (zebrafish)	pl10	NP_571016.2

Gene Ontology
Bibliography

Related articles in PubMed

Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.
Inoue H, Ogonuki N, Hirose M, Hatanaka Y, Matoba S, Chuma S, Kobayashi K, Wakana S, Noguchi J, Inoue K, Tanemura K, Ogura A
Biochemical and biophysical research communications478(2)592-8(2016 09)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Structural characterization and expression studies of Dby and its homologs in the mouse.
Vong QP, Li Y, Lau YF, Dym M, Rennert OM, Chan WY
Journal of andrology27(5)653-61(2006 Sep-Oct)

Characterization of D1Pas1, a mouse autosomal homologue of the human AZFa region DBY, as a nuclear protein in spermatogenic cells.
Session DR, Lee GS, Wolgemuth DJ
Fertility and sterility76(4)804-11(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deletion of D1Pas1 did not cause noticeable embryonic defects or death, indicating that D1Pas1 is not essential for embryogenesis. Whereas homozygous knockout female mice showed normal reproductive performance, homozygous knockout male mice were completely sterile. mouse D1Pas1 is the first-identified DEAD-box RNA helicase that plays critical roles in the final step of the first meiotic prophase in male germ cells.
Title: Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.

unlike DBY in humans, the role of Dby in spermatogenesis is less obvious in the mouse and its biologic activity may be replaced by that of Ddx3 and D1Pas1.
Title: Structural characterization and expression studies of Dby and its homologs in the mouse.

The following D1Pas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the D1Pas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00009	NM_033077.3 Latest version!	Mus musculus DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

D1Pas1 ( NM_033077.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_149068.1 Mus musculus DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1), mRNA.

Price & Availability↑

CloneID	OMu00009
Clone ID Related Accession (Same CDS sequence)	NM_033077.3
Accession Version	NM_033077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	putative ATP-dependent RNA helicase Pl10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029542.1 and BG070553.2. On Oct 12, 2012 this sequence version replaced NM_033077.2. ##Evidence-Data-START## Transcript is intronless :: AK029542.1 [ECO:0000345] ##Evidence-Data-END##

ATGAGTCATG TGGCAGAGGA AGATGAGCTC GGGCTGGACC AGCAGTTGGC TGGCCTAGAC 
CTGACGTCTC GGGACAGCCA GAGCGGGGGC AGCACAGCCA GCAAAGGGCG CTACATCCCC 
CCTCACCTGC GGAACCGGGA GGCCGCGAAG GCGTTCTACG ACAAAGACGG CTCACGGTGG 
AGTAAAGACA AGGATGCCTA CAGCAGCTTC GGGTCCCGCA GCGACACGAG AGCCAAGTCC 
AGTTTCTTCA GTGACCGCGG CGGAAGCGGC TCCAGGGGAA GGTTTGACGA GCGCGGGCGG 
AGTGACTACG AGAGTGTTGG CAGCCGGGGA GGTAGAAGTG GCTTTGGCAA ATTTGAGCGG 
GGCGGGAACA GTCGCTGGTG TGACAAAGCA GACGAGGATG ACTGGTCGAA ACCGCTCCCA 
CCAAGCGAAC GCTTGGAACA GGAACTCTTT TCCGGAGGCA ACACTGGGAT TAACTTTGAG 
AAATATGATG ACATCCCGGT TGAGGCGACA GGGAATAACT GCCCTCCACA TATTGAAAGT 
TTCAGCGATG TCGAGATGGG AGAGATTATT ATGGGAAACA TCGAGCTCAC TCGTTACACA 
CGCCCAACTC CTGTGCAAAA GCATGCTATT CCTATTATCA AGGAGAAAAG AGACTTGATG 
GCTTGTGCCC AAACGGGGTC TGGAAAAACA GCAGCTTTTC TTCTACCTAT CTTGAGTCAG 
ATCTATACGG ATGGTCCAGG TGAGGCTTTG AGGGCCATGA AGGAGAATGG AAAGTATGGG 
CGTCGCAAAC AGTATCCAAT CTCCTTGGTC TTGGCTCCGA CCAGAGAATT GGCCGTGCAG 
ATCTATGAGG AGGCCAGGAA ATTCTCATAC CGCTCTCGAG TTCGTCCTTG TGTGGTTTAT 
GGTGGAGCTG ATATTGGGCA GCAGATTCGA GACTTGGAAC GTGGCTGTCA CTTGTTAGTA 
GCTACTCCAG GACGGCTAGT AGATATGATG GAGAGAGGAA AGATTGGACT GGACTTCTGC 
AAATACTTGG TGTTAGATGA AGCAGATCGA ATGTTGGACA TGGGCTTTGA GCCTCAGATA 
CGAAGAATAG TTGAGCAAGA CACCATGCCT CCAAAAGGTG TCCGCCACAC TATGATGTTT 
AGTGCTACCT TTCCTAAGGA AATACAGATG CTGGCTCGGG ATTTCTTGGA TGAATATATC 
TTTCTGGCCG TAGGAAGAGT TGGGTCTACT TCTGAGAACA TCACACAGAA AGTCGTGTGG 
GTGGAAGAAG CAGACAAGCG GTCATTTCTG CTTGACCTCC TGAATGCAAC GGGCAAGGAT 
TCCCTGATAT TGGTGTTTGT GGAAACCAAA AAGGGTGCAG ATTCTCTGGA GGACTTCTTA 
TACCACGAAG GATATGCTTG TACCAGTATC CATGGAGACC GATCTCAGCG AGATAGGGAA 
GAGGCCCTTC ACCAATTCCG ATCAGGAAAA AGCCCAATTC TCGTGGCTAC AGCAGTAGCT 
GCAAGAGGAC TGGATATTTC CAATGTGAAA CATGTCATCA ATTTCGACTT GCCTAGTGAC 
ATCGAAGAAT ATGTGCATCG CATTGGCCGT ACAGGTCGTG TTGGAAACCT TGGCCTTGCC 
ACCTCATTCT TCAACGAGAG GAACATAAAT ATTACCAAAG ATTTATTGGA TCTTCTTGTT 
GAAGCAAAAC AAGAAGTGCC ATCTTGGCTA GAAAACATGG CTTTTGAACA CCATTACAAG 
GGTGGCAGTC GTGGACGGTC CAAGAGCAGA TTTAGTGGAG GATTTGGTGC TAGAGACTAT 
CGACAAAGTA GCGGTGCCAG CAGTTCCAGC TTCAGCAGTG GCCGTGCTAG CAACAGTCGC 
AGCGGTGGAG GCAGCCATGG CAGCAGCAGA GGGTTTGGTG GAGGTAGCTA TGGAGGCTTT 
TACAACAGTG ATGGATATGG AGGAAATTAT AGCTCCCAGG GGGTTGACTG GTGGGGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149068.1
CDS	461..2443
Translation	MSHVAEEDELGLDQQLAGLDLTSRDSQSGGSTASKGRYIPPHLRNREAAKAFYDKDGSRWSKDKDAYSSFGSRSDTRAKSSFFSDRGGSGSRGRFDERGRSDYESVGSRGGRSGFGKFERGGNSRWCDKADEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALRAMKENGKYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEADKRSFLLDLLNATGKDSLILVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGGSRGRSKSRFSGGFGARDYRQSSGASSSSFSSGRASNSRSGGGSHGSSRGFGGGSYGGFYNSDGYGGNYSSQGVDWWGN

Target ORF information:

RefSeq Version	NM_033077.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033077.3

ORF Insert Sequence:

ATGAGTCATG TGGCAGAGGA AGATGAGCTC GGGCTGGACC AGCAGTTGGC TGGCCTAGAC 
CTGACGTCTC GGGACAGCCA GAGCGGGGGC AGCACAGCCA GCAAAGGGCG CTACATCCCC 
CCTCACCTGC GGAACCGGGA GGCCGCGAAG GCGTTCTACG ACAAAGACGG CTCACGGTGG 
AGTAAAGACA AGGATGCCTA CAGCAGCTTC GGGTCCCGCA GCGACACGAG AGCCAAGTCC 
AGTTTCTTCA GTGACCGCGG CGGAAGCGGC TCCAGGGGAA GGTTTGACGA GCGCGGGCGG 
AGTGACTACG AGAGTGTTGG CAGCCGGGGA GGTAGAAGTG GCTTTGGCAA ATTTGAGCGG 
GGCGGGAACA GTCGCTGGTG TGACAAAGCA GACGAGGATG ACTGGTCGAA ACCGCTCCCA 
CCAAGCGAAC GCTTGGAACA GGAACTCTTT TCCGGAGGCA ACACTGGGAT TAACTTTGAG 
AAATATGATG ACATCCCGGT TGAGGCGACA GGGAATAACT GCCCTCCACA TATTGAAAGT 
TTCAGCGATG TCGAGATGGG AGAGATTATT ATGGGAAACA TCGAGCTCAC TCGTTACACA 
CGCCCAACTC CTGTGCAAAA GCATGCTATT CCTATTATCA AGGAGAAAAG AGACTTGATG 
GCTTGTGCCC AAACGGGGTC TGGAAAAACA GCAGCTTTTC TTCTACCTAT CTTGAGTCAG 
ATCTATACGG ATGGTCCAGG TGAGGCTTTG AGGGCCATGA AGGAGAATGG AAAGTATGGG 
CGTCGCAAAC AGTATCCAAT CTCCTTGGTC TTGGCTCCGA CCAGAGAATT GGCCGTGCAG 
ATCTATGAGG AGGCCAGGAA ATTCTCATAC CGCTCTCGAG TTCGTCCTTG TGTGGTTTAT 
GGTGGAGCTG ATATTGGGCA GCAGATTCGA GACTTGGAAC GTGGCTGTCA CTTGTTAGTA 
GCTACTCCAG GACGGCTAGT AGATATGATG GAGAGAGGAA AGATTGGACT GGACTTCTGC 
AAATACTTGG TGTTAGATGA AGCAGATCGA ATGTTGGACA TGGGCTTTGA GCCTCAGATA 
CGAAGAATAG TTGAGCAAGA CACCATGCCT CCAAAAGGTG TCCGCCACAC TATGATGTTT 
AGTGCTACCT TTCCTAAGGA AATACAGATG CTGGCTCGGG ATTTCTTGGA TGAATATATC 
TTTCTGGCCG TAGGAAGAGT TGGGTCTACT TCTGAGAACA TCACACAGAA AGTCGTGTGG 
GTGGAAGAAG CAGACAAGCG GTCATTTCTG CTTGACCTCC TGAATGCAAC GGGCAAGGAT 
TCCCTGATAT TGGTGTTTGT GGAAACCAAA AAGGGTGCAG ATTCTCTGGA GGACTTCTTA 
TACCACGAAG GATATGCTTG TACCAGTATC CATGGAGACC GATCTCAGCG AGATAGGGAA 
GAGGCCCTTC ACCAATTCCG ATCAGGAAAA AGCCCAATTC TCGTGGCTAC AGCAGTAGCT 
GCAAGAGGAC TGGATATTTC CAATGTGAAA CATGTCATCA ATTTCGACTT GCCTAGTGAC 
ATCGAAGAAT ATGTGCATCG CATTGGCCGT ACAGGTCGTG TTGGAAACCT TGGCCTTGCC 
ACCTCATTCT TCAACGAGAG GAACATAAAT ATTACCAAAG ATTTATTGGA TCTTCTTGTT 
GAAGCAAAAC AAGAAGTGCC ATCTTGGCTA GAAAACATGG CTTTTGAACA CCATTACAAG 
GGTGGCAGTC GTGGACGGTC CAAGAGCAGA TTTAGTGGAG GATTTGGTGC TAGAGACTAT 
CGACAAAGTA GCGGTGCCAG CAGTTCCAGC TTCAGCAGTG GCCGTGCTAG CAACAGTCGC 
AGCGGTGGAG GCAGCCATGG CAGCAGCAGA GGGTTTGGTG GAGGTAGCTA TGGAGGCTTT 
TACAACAGTG ATGGATATGG AGGAAATTAT AGCTCCCAGG GGGTTGACTG GTGGGGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.
Biochemical and biophysical research communications478(2)592-8(2016 09)
Inoue H,Ogonuki N,Hirose M,Hatanaka Y,Matoba S,Chuma S,Kobayashi K,Wakana S,Noguchi J,Inoue K,Tanemura K,Ogura A

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Structural characterization and expression studies of Dby and its homologs in the mouse.
Journal of andrology27(5)653-61(2006 Sep-Oct)
Vong QP,Li Y,Lau YF,Dym M,Rennert OM,Chan WY

Characterization of D1Pas1, a mouse autosomal homologue of the human AZFa region DBY, as a nuclear protein in spermatogenic cells.
Fertility and sterility76(4)804-11(2001 Oct)
Session DR,Lee GS,Wolgemuth DJ