Ambra1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ambra1
Entrez Gene ID	110309853
Full Name	autophagy and beclin 1 regulator 1
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021182585.1	XP_021038244.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_021182598.1	XP_021038257.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_021182598.2	XP_021038257.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_021182592.2	XP_021038251.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_021182606.2	XP_021038265.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_021182592.1	XP_021038251.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_021182606.1	XP_021038265.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_021182585.2	XP_021038244.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following Ambra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ambra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv37658	XM_021182585.2 Latest version!	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv37659	XM_021182592.2 Latest version!	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv37660	XM_021182598.2 Latest version!	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv37661	XM_021182606.2 Latest version!	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OMv37660	XM_021182598.1	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv37659	XM_021182592.1	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv37661	XM_021182606.1	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMv37658	XM_021182585.1	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ambra1 ( XM_021182585.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038244.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv37658
Clone ID Related Accession (Same CDS sequence)	XM_021182585.1 , XM_021182585.2
Accession Version	XM_021182585.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038244.1
CDS	471..4373
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182585.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182585.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182585.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038244.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv37658
Clone ID Related Accession (Same CDS sequence)	XM_021182585.1 , XM_021182585.2
Accession Version	XM_021182585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021182585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038244.1
CDS	460..4362
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182585.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182585.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182592.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038251.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

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CloneID	OMv37659
Clone ID Related Accession (Same CDS sequence)	XM_021182592.2 , XM_021182592.1
Accession Version	XM_021182592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021182592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGCC AACTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTGCTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTAC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCT GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACCTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC ATGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGTTGCAAC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGTACCCA GACTCTGGCC 
CTCCAGTTGC AGAATGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCT CTCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCCAGTACC TCTGGGAGGG AACTACCCCA GGCAGCCCTG 
TCCTCTTCCT CCCCTGTTCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CCGGGGGGAA 
GCTGCAGGCC CTAGTGGGGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038251.1
CDS	460..4275
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182592.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182592.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGCC AACTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTGCTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTAC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCT GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACCTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC ATGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGTTGCAAC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGTACCCA GACTCTGGCC 
CTCCAGTTGC AGAATGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCT CTCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCCAGTACC TCTGGGAGGG AACTACCCCA GGCAGCCCTG 
TCCTCTTCCT CCCCTGTTCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CCGGGGGGAA 
GCTGCAGGCC CTAGTGGGGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182592.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038251.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv37659
Clone ID Related Accession (Same CDS sequence)	XM_021182592.2 , XM_021182592.1
Accession Version	XM_021182592.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGCC AACTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTGCTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTAC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCT GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACCTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC ATGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGTTGCAAC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGTACCCA GACTCTGGCC 
CTCCAGTTGC AGAATGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCT CTCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCCAGTACC TCTGGGAGGG AACTACCCCA GGCAGCCCTG 
TCCTCTTCCT CCCCTGTTCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CCGGGGGGAA 
GCTGCAGGCC CTAGTGGGGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038251.1
CDS	471..4286
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182592.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182592.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCCCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCGT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTT CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTTCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GACACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGCGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTA TGAGGGCATG CCGCCAAGTG GCAGCCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAACAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGCC AACTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTGCTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTAC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCT GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACCTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC ATGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGTTGCAAC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGTACCCA GACTCTGGCC 
CTCCAGTTGC AGAATGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCT CTCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCCAGTACC TCTGGGAGGG AACTACCCCA GGCAGCCCTG 
TCCTCTTCCT CCCCTGTTCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CCGGGGGGAA 
GCTGCAGGCC CTAGTGGGGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182598.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038257.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMv37660
Clone ID Related Accession (Same CDS sequence)	XM_021182598.1 , XM_021182598.2
Accession Version	XM_021182598.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAACTCC TGGCAGCCTT CATCCCGAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTACAACAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC 
ATCAACTCTG CCCGTTGGCT ACCTGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC 
CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGTTG 
CAACCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGTA CCCAGACTCT GGCCCTCCAG 
TTGCAGAATG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA 
GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG 
ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC 
ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TGCTCTCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCCAG TACCTCTGGG AGGGAACTAC CCCAGGCAGC CCTGTCCTCT 
TCCTCCCCTG TTCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACCGGGG GGAAGCTGCA 
GGCCCTAGTG GGGAGCCACA GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038257.1
CDS	471..4100
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182598.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182598.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAACTCC TGGCAGCCTT CATCCCGAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTACAACAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC 
ATCAACTCTG CCCGTTGGCT ACCTGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC 
CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGTTG 
CAACCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGTA CCCAGACTCT GGCCCTCCAG 
TTGCAGAATG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA 
GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG 
ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC 
ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TGCTCTCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCCAG TACCTCTGGG AGGGAACTAC CCCAGGCAGC CCTGTCCTCT 
TCCTCCCCTG TTCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACCGGGG GGAAGCTGCA 
GGCCCTAGTG GGGAGCCACA GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182598.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038257.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.

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CloneID	OMv37660
Clone ID Related Accession (Same CDS sequence)	XM_021182598.1 , XM_021182598.2
Accession Version	XM_021182598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021182598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAACTCC TGGCAGCCTT CATCCCGAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTACAACAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC 
ATCAACTCTG CCCGTTGGCT ACCTGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC 
CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGTTG 
CAACCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGTA CCCAGACTCT GGCCCTCCAG 
TTGCAGAATG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA 
GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG 
ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC 
ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TGCTCTCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCCAG TACCTCTGGG AGGGAACTAC CCCAGGCAGC CCTGTCCTCT 
TCCTCCCCTG TTCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACCGGGG GGAAGCTGCA 
GGCCCTAGTG GGGAGCCACA GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038257.1
CDS	459..4088
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182598.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182598.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAACTCC TGGCAGCCTT CATCCCGAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTACAACAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC 
ATCAACTCTG CCCGTTGGCT ACCTGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC 
CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGTTG 
CAACCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGTA CCCAGACTCT GGCCCTCCAG 
TTGCAGAATG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA 
GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG 
ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC 
ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TGCTCTCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCCAG TACCTCTGGG AGGGAACTAC CCCAGGCAGC CCTGTCCTCT 
TCCTCCCCTG TTCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACCGGGG GGAAGCTGCA 
GGCCCTAGTG GGGAGCCACA GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182606.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038265.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.

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CloneID	OMv37661
Clone ID Related Accession (Same CDS sequence)	XM_021182606.2 , XM_021182606.1
Accession Version	XM_021182606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021182606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038265.1
CDS	459..4001
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182606.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182606.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_021182606.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021038265.1 Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMv37661
Clone ID Related Accession (Same CDS sequence)	XM_021182606.2 , XM_021182606.1
Accession Version	XM_021182606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034571.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021038265.1
CDS	471..4013
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFSSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETSQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTYEGMPPSGSQLPPLERTEGQMPSSSRLELNSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERALSPRTASWDQPSTSGRELPQAALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_021182606.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021182606.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTG CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GTTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTCCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTCGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCACTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCCCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCGTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTTCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTTCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAC ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GCGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTATGA GGGCATGCCG 
CCAAGTGGCA GCCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAACAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAAC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTACAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCTGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG TTGCAACCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GTACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC CAGTACCTCT 
GGGAGGGAAC TACCCCAGGC AGCCCTGTCC TCTTCCTCCC CTGTTCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACCG GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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[email protected]

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

Ambra1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム