Tpcn1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpcn1
Entrez Gene ID	110294094
Full Name	two pore segment channel 1
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021162092.1	XP_021017751.1	two pore calcium channel protein 1 isoform X1
XM_021162093.1	XP_021017752.1	two pore calcium channel protein 1 isoform X1
XM_021162094.1	XP_021017753.1	two pore calcium channel protein 1 isoform X2
XM_021162095.1	XP_021017754.1	two pore calcium channel protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following Tpcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv00717	XM_021162093.1 Latest version!	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMv00717	XM_021162095.1 Latest version!	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMv00717	XM_021162092.1 Latest version!	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMv00718	XM_021162094.1 Latest version!	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpcn1 ( XM_021162092.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021017751.1 Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv00717
Clone ID Related Accession (Same CDS sequence)	XM_021162092.1 , XM_021162093.1 , XM_021162095.1
Accession Version	XM_021162092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034574.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGTCCGGC 
CCCGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021017751.1
CDS	279..2732
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSVHNSQVPSLVSRADSPHSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFYTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLANQRRPTGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKTKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDGGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVSGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_021162092.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021162092.1

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGTCCGGC 
CCCGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpcn1 ( XM_021162093.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021017752.1 Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv00717
Clone ID Related Accession (Same CDS sequence)	XM_021162092.1 , XM_021162093.1 , XM_021162095.1
Accession Version	XM_021162093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034574.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGTCCGGC 
CCCGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021017752.1
CDS	255..2708
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSVHNSQVPSLVSRADSPHSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFYTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLANQRRPTGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKTKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDGGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVSGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_021162093.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021162093.1

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGTCCGGC 
CCCGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpcn1 ( XM_021162095.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021017754.1 Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X2, mRNA.

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CloneID	OMv00717
Clone ID Related Accession (Same CDS sequence)	XM_021162092.1 , XM_021162093.1 , XM_021162095.1
Accession Version	XM_021162095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034574.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGTCCGGC 
CCCGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021017754.1
CDS	294..2747
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSVHNSQVPSLVSRADSPHSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFYTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLANQRRPTGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKTKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDGGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVSGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_021162095.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021162095.1

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGTCCGGC 
CCCGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACCGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpcn1 ( XM_021162094.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021017753.1 Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMv00718
Clone ID Related Accession (Same CDS sequence)	XM_021162094.1
Accession Version	XM_021162094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	two pore calcium channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034574.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGGAGT GGTACGAGGA GCATGCCCGG 
GAACAGGAGC AGCAGAAGCT CAGGGGCAGC GTGTCCGGCC CCGCAGCCCA GCAGCCCCCT 
GGCAGTCGCC AGCGCTCCCA GACCGTCACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021017753.1
CDS	279..2651
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSVHNSQVPSLVSRADSPHSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFYTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLANQRRPTGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKTKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDGGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQEWYEEHAREQEQQKLRGSVSGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_021162094.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli two pore segment channel 1 (Tpcn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021162094.1

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCCAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGAGGC 
AGCCACAGCG TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCAGAGCGGA CAGCCCCCAC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCTACAC GTTCGTCCGG CACAAACGCA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG TATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTGCGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTG CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT CGCCATCCTC GGTTTCTACT TATTCTCCAC GAACCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTTGTTCT CCTGACCACA 
GCCAACTTTC CAGACGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGTGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCTGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCTTA CGGCCTGCTT GCCAACCAAC GGAGGCCGAC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAACCCC GGATGAGTGC AAGAGAACGC 
TTCCTGACTT TCAAGGCCTT AAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGACAA AGAAGAACAG ACAGCATTGG 
TTTGATGAGC TTCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAATGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGCGGGAAT TTCACCTCGA AGCATGTGCC CTGGAGTTAC 
CTTGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACAGC ATTCGCCTTC 
CTGGGACTGC TTGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTCGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTTA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACGG TGGCCGATGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTTGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATTG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CGGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TTCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTACTGGACA CCCTCTCTCA GATGGAGAAA TACCAGGAGT GGTACGAGGA GCATGCCCGG 
GAACAGGAGC AGCAGAAGCT CAGGGGCAGC GTGTCCGGCC CCGCAGCCCA GCAGCCCCCT 
GGCAGTCGCC AGCGCTCCCA GACCGTCACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Tpcn1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム