Nefl cDNA ORF clone, Microtus ochrogaster(prairie vole)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nefl
Entrez Gene ID	101993439
Full Name	neurofilament light
Gene Type	protein-coding
Organism	Microtus ochrogaster(prairie vole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005355464.3	XP_005355521.1	neurofilament light polypeptide
XM_005355464.2	XP_005355521.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology

The following Nefl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nefl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMi109703	XM_005355464.3 Latest version!	Microtus ochrogaster neurofilament light (Nefl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OMi109703	XM_005355464.2	Microtus ochrogaster neurofilament, light polypeptide (Nefl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nefl ( XM_005355464.3 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_005355521.1 Microtus ochrogaster neurofilament light (Nefl), mRNA.

Price & Availability↑

CloneID	OMi109703
Clone ID Related Accession (Same CDS sequence)	XM_005355464.3 , XM_005355464.2
Accession Version	XM_005355464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022019.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_005355464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCGT TCGGCTACGA CCCGTACTTT TCAACCTCCT ACAAGAGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACTGCGCG TTCCGCGTAC 
TCCAGCTACT CCGCGCCGGT CTCTTCCTCT CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCGGGCTCTT TGATGCCCAG CTTGGAGAAT CTCGACCTGA GTCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCCCAGC TCCAGGACCT CAACGATCGC 
TTCGCCAGCT TCATTGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGTTGG TGCTGCGCCA GAAACACTCG GAGCCGTCCC GCTTCCGCGC CCTGTACGAG 
CAGGAGATCC GTGATCTGCG GCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAGGGGCT GGAGGAAACT CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTAA GTCGCGAGGA CGCCGAGGGT CGGCTGATGG AGGCCCGCAA AGGCGCCGAT 
GAGGCTGCGC TCGCCCGTGC CGAGCTGGAG AAGCGCATCG ACAGCCTGAT GGACGAGATC 
GCCTTCCTGA AGAAGGTGCA CGAGGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAT 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC AGCCCTCAAG 
GACATCCGCG CTCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACAGT GCTGACCGAG AGCGCCGCCA AGAACACAGA TGCTGTGCGC 
GCTGCCAAGG ACGAGGTGTC CGAAAGCCGC CGCCTGCTCA AGGCTAAGAC CCTGGAGATC 
GAAGCATGCA GGGGTATGAA CGAAGCTCTG GAGAAGCAGC TGCAGGAGTT GGAGGACAAG 
CAGAACGCAG ACATCAGTGC CATGCAGGAC ACAATCAACA AGTTAGAGAA CGAGCTGAGA 
AGCACGAAGA GCGAGATGGC GAGGTACCTG AAGGAGTACC AGGACCTCCT CAACGTGAAG 
ATGGCCTTGG ACATCGAGAT TGCGGCTTAC AGGAAACTCT TGGAAGGCGA AGAGACCAGG 
CTCAGTTTCA CCAGCGTGGG CAGCCTAACC AGTGGCTACT CTCAGAGCTC GCAGATCTTT 
GGCCGCTCCG CCTACAGTGG CTTGCAGAGC AGCTCCTACT TGATGTCGGC TCGCTCGTTC 
CCAGCCTACT ACACCAGCCA CGTCCAGGAA GAGCAGACAG AGGTGGAGGA GACCATCGAG 
GCTACCAAAG CTGAGGAGGC CAAAGATGAA CCCCCTTCTG AGGGAGAAGC AGAAGAGGAG 
GAGAAGGAGA AGGAGGAAGG AGAGGAAGAG GAAGGTGCTG AGGAGGAGGA AGCTGCCAAG 
GATGAGTCTG AAGACACGAA AGAAGAAGAA GAAGGTGGTG AGGGTGAAGA GGAAGACACC 
AAAGAATCTG AAGAAGAGGA GAAGAAAGAC GGAGGTGCTG GGGAGGAGCA AGCAACTAAG 
AAGAAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005355521.1
CDS	95..1726
Translation	MSSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSLTSGYSQSSQIFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKDGGAGEEQATKKKD

Target ORF information:

RefSeq Version	XM_005355464.3
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster neurofilament light (Nefl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005355464.3

ORF Insert Sequence:

ATGAGTTCGT TCGGCTACGA CCCGTACTTT TCAACCTCCT ACAAGAGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACTGCGCG TTCCGCGTAC 
TCCAGCTACT CCGCGCCGGT CTCTTCCTCT CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCGGGCTCTT TGATGCCCAG CTTGGAGAAT CTCGACCTGA GTCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCCCAGC TCCAGGACCT CAACGATCGC 
TTCGCCAGCT TCATTGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGTTGG TGCTGCGCCA GAAACACTCG GAGCCGTCCC GCTTCCGCGC CCTGTACGAG 
CAGGAGATCC GTGATCTGCG GCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAGGGGCT GGAGGAAACT CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTAA GTCGCGAGGA CGCCGAGGGT CGGCTGATGG AGGCCCGCAA AGGCGCCGAT 
GAGGCTGCGC TCGCCCGTGC CGAGCTGGAG AAGCGCATCG ACAGCCTGAT GGACGAGATC 
GCCTTCCTGA AGAAGGTGCA CGAGGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAT 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC AGCCCTCAAG 
GACATCCGCG CTCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACAGT GCTGACCGAG AGCGCCGCCA AGAACACAGA TGCTGTGCGC 
GCTGCCAAGG ACGAGGTGTC CGAAAGCCGC CGCCTGCTCA AGGCTAAGAC CCTGGAGATC 
GAAGCATGCA GGGGTATGAA CGAAGCTCTG GAGAAGCAGC TGCAGGAGTT GGAGGACAAG 
CAGAACGCAG ACATCAGTGC CATGCAGGAC ACAATCAACA AGTTAGAGAA CGAGCTGAGA 
AGCACGAAGA GCGAGATGGC GAGGTACCTG AAGGAGTACC AGGACCTCCT CAACGTGAAG 
ATGGCCTTGG ACATCGAGAT TGCGGCTTAC AGGAAACTCT TGGAAGGCGA AGAGACCAGG 
CTCAGTTTCA CCAGCGTGGG CAGCCTAACC AGTGGCTACT CTCAGAGCTC GCAGATCTTT 
GGCCGCTCCG CCTACAGTGG CTTGCAGAGC AGCTCCTACT TGATGTCGGC TCGCTCGTTC 
CCAGCCTACT ACACCAGCCA CGTCCAGGAA GAGCAGACAG AGGTGGAGGA GACCATCGAG 
GCTACCAAAG CTGAGGAGGC CAAAGATGAA CCCCCTTCTG AGGGAGAAGC AGAAGAGGAG 
GAGAAGGAGA AGGAGGAAGG AGAGGAAGAG GAAGGTGCTG AGGAGGAGGA AGCTGCCAAG 
GATGAGTCTG AAGACACGAA AGAAGAAGAA GAAGGTGGTG AGGGTGAAGA GGAAGACACC 
AAAGAATCTG AAGAAGAGGA GAAGAAAGAC GGAGGTGCTG GGGAGGAGCA AGCAACTAAG 
AAGAAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nefl ( XM_005355464.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_005355521.1 Microtus ochrogaster neurofilament, light polypeptide (Nefl), mRNA.

Price & Availability↑

CloneID	OMi109703
Clone ID Related Accession (Same CDS sequence)	XM_005355464.3 , XM_005355464.2
Accession Version	XM_005355464.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004949020.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2015 this sequence version replaced XM_005355464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCGT TCGGCTACGA CCCGTACTTT TCAACCTCCT ACAAGAGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACTGCGCG TTCCGCGTAC 
TCCAGCTACT CCGCGCCGGT CTCTTCCTCT CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCGGGCTCTT TGATGCCCAG CTTGGAGAAT CTCGACCTGA GTCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCCCAGC TCCAGGACCT CAACGATCGC 
TTCGCCAGCT TCATTGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGTTGG TGCTGCGCCA GAAACACTCG GAGCCGTCCC GCTTCCGCGC CCTGTACGAG 
CAGGAGATCC GTGATCTGCG GCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAGGGGCT GGAGGAAACT CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTAA GTCGCGAGGA CGCCGAGGGT CGGCTGATGG AGGCCCGCAA AGGCGCCGAT 
GAGGCTGCGC TCGCCCGTGC CGAGCTGGAG AAGCGCATCG ACAGCCTGAT GGACGAGATC 
GCCTTCCTGA AGAAGGTGCA CGAGGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAT 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC AGCCCTCAAG 
GACATCCGCG CTCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACAGT GCTGACCGAG AGCGCCGCCA AGAACACAGA TGCTGTGCGC 
GCTGCCAAGG ACGAGGTGTC CGAAAGCCGC CGCCTGCTCA AGGCTAAGAC CCTGGAGATC 
GAAGCATGCA GGGGTATGAA CGAAGCTCTG GAGAAGCAGC TGCAGGAGTT GGAGGACAAG 
CAGAACGCAG ACATCAGTGC CATGCAGGAC ACAATCAACA AGTTAGAGAA CGAGCTGAGA 
AGCACGAAGA GCGAGATGGC GAGGTACCTG AAGGAGTACC AGGACCTCCT CAACGTGAAG 
ATGGCCTTGG ACATCGAGAT TGCGGCTTAC AGGAAACTCT TGGAAGGCGA AGAGACCAGG 
CTCAGTTTCA CCAGCGTGGG CAGCCTAACC AGTGGCTACT CTCAGAGCTC GCAGATCTTT 
GGCCGCTCCG CCTACAGTGG CTTGCAGAGC AGCTCCTACT TGATGTCGGC TCGCTCGTTC 
CCAGCCTACT ACACCAGCCA CGTCCAGGAA GAGCAGACAG AGGTGGAGGA GACCATCGAG 
GCTACCAAAG CTGAGGAGGC CAAAGATGAA CCCCCTTCTG AGGGAGAAGC AGAAGAGGAG 
GAGAAGGAGA AGGAGGAAGG AGAGGAAGAG GAAGGTGCTG AGGAGGAGGA AGCTGCCAAG 
GATGAGTCTG AAGACACGAA AGAAGAAGAA GAAGGTGGTG AGGGTGAAGA GGAAGACACC 
AAAGAATCTG AAGAAGAGGA GAAGAAAGAC GGAGGTGCTG GGGAGGAGCA AGCAACTAAG 
AAGAAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005355521.1
CDS	165..1796
Translation	MSSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSLTSGYSQSSQIFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKDGGAGEEQATKKKD

Target ORF information:

RefSeq Version	XM_005355464.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster neurofilament, light polypeptide (Nefl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005355464.2

ORF Insert Sequence:

ATGAGTTCGT TCGGCTACGA CCCGTACTTT TCAACCTCCT ACAAGAGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACTGCGCG TTCCGCGTAC 
TCCAGCTACT CCGCGCCGGT CTCTTCCTCT CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCGGGCTCTT TGATGCCCAG CTTGGAGAAT CTCGACCTGA GTCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCCCAGC TCCAGGACCT CAACGATCGC 
TTCGCCAGCT TCATTGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGTTGG TGCTGCGCCA GAAACACTCG GAGCCGTCCC GCTTCCGCGC CCTGTACGAG 
CAGGAGATCC GTGATCTGCG GCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAGGGGCT GGAGGAAACT CTGCGCAACC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTAA GTCGCGAGGA CGCCGAGGGT CGGCTGATGG AGGCCCGCAA AGGCGCCGAT 
GAGGCTGCGC TCGCCCGTGC CGAGCTGGAG AAGCGCATCG ACAGCCTGAT GGACGAGATC 
GCCTTCCTGA AGAAGGTGCA CGAGGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAT 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC AGCCCTCAAG 
GACATCCGCG CTCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAATGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACAGT GCTGACCGAG AGCGCCGCCA AGAACACAGA TGCTGTGCGC 
GCTGCCAAGG ACGAGGTGTC CGAAAGCCGC CGCCTGCTCA AGGCTAAGAC CCTGGAGATC 
GAAGCATGCA GGGGTATGAA CGAAGCTCTG GAGAAGCAGC TGCAGGAGTT GGAGGACAAG 
CAGAACGCAG ACATCAGTGC CATGCAGGAC ACAATCAACA AGTTAGAGAA CGAGCTGAGA 
AGCACGAAGA GCGAGATGGC GAGGTACCTG AAGGAGTACC AGGACCTCCT CAACGTGAAG 
ATGGCCTTGG ACATCGAGAT TGCGGCTTAC AGGAAACTCT TGGAAGGCGA AGAGACCAGG 
CTCAGTTTCA CCAGCGTGGG CAGCCTAACC AGTGGCTACT CTCAGAGCTC GCAGATCTTT 
GGCCGCTCCG CCTACAGTGG CTTGCAGAGC AGCTCCTACT TGATGTCGGC TCGCTCGTTC 
CCAGCCTACT ACACCAGCCA CGTCCAGGAA GAGCAGACAG AGGTGGAGGA GACCATCGAG 
GCTACCAAAG CTGAGGAGGC CAAAGATGAA CCCCCTTCTG AGGGAGAAGC AGAAGAGGAG 
GAGAAGGAGA AGGAGGAAGG AGAGGAAGAG GAAGGTGCTG AGGAGGAGGA AGCTGCCAAG 
GATGAGTCTG AAGACACGAA AGAAGAAGAA GAAGGTGGTG AGGGTGAAGA GGAAGACACC 
AAAGAATCTG AAGAAGAGGA GAAGAAAGAC GGAGGTGCTG GGGAGGAGCA AGCAACTAAG 
AAGAAAGATT GA