Tsc2 gene cDNA ORF clone, Microtus ochrogaster(prairie vole)

mRNA	Protein	Name
XM_013347152.1	XP_013202606.1	tuberin isoform X2
XM_013347153.2	XP_013202607.1	tuberin isoform X4
XM_005349161.2	XP_005349218.1	tuberin isoform X1
XM_013347155.2	XP_013202609.1	tuberin isoform X6
XM_005349161.1	XP_005349218.1	tuberin isoform X1
XM_013347154.1	XP_013202608.1	tuberin isoform X4
XM_013347155.1	XP_013202609.1	tuberin isoform X5
XM_013347152.2	XP_013202606.1	tuberin isoform X3
XM_013347153.1	XP_013202607.1	tuberin isoform X3
XM_013347156.1	XP_013202610.1	tuberin isoform X6
XM_026780898.1	XP_026636699.1	tuberin isoform X2
XM_013347156.2	XP_013202610.1	tuberin isoform X7
XM_013347154.2	XP_013202608.1	tuberin isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMi104122	XM_005349161.2 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104123	XM_013347152.2 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104124	XM_013347153.2 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104125	XM_013347154.2 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104126	XM_013347155.2 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104127	XM_013347156.2 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi170855	XM_026780898.1 Latest version!	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMi104122	XM_005349161.1	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104126	XM_013347155.1	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104123	XM_013347152.1	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104124	XM_013347153.1	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104127	XM_013347156.1	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi104125	XM_013347154.1	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

Tsc2 ( XM_005349161.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_005349218.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMi104122
Clone ID Related Accession (Same CDS sequence)	XM_005349161.2 , XM_005349161.1
Accession Version	XM_005349161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_005349161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCTGC CGTGGTTCTC 
GAGGAAGGAA GTCCAGGAGA GGCTCATGTA CCAGTGGAGC CCCCCGAGTT GGAGGATTTT 
GAGGCAGTAG GTACAGACAG GCACTGCCAG CGTCCTGACA CCTACAGCAG GTCGTCCTCA 
GCGTCTAGCC AGGAAGAAAA GTCCCAGCTG GAGGAGATGG CTGCCGGAGG CATCCCCATC 
GAGCGGGCCA TCTCCGAGGG TGCTCGGCCT GCTGTGGACC TCTCCTTCCA ACCCTCACAG 
CCCCTGAGCA AGTCTAGCTC TTCTCCTGAG TTGCAGACCT TGCAAGACAT CCTCGGAGAC 
CTAGGGGACA AGGCTGACAT TGGACGGCTG AGTCCTGAGT CTAAGGTCCG GTCCCAGTCA 
GGGATCCTGG ATGGGGAAGT GGCTACCTGG TCAGCCCCAG GTGAAGAAGG CCGTGTCACA 
GTCCCACCTG AAGGTCCCCT GCCTTCCAGT TCTCCCCGCT CTCCTAGTGG TCTCCGGCCC 
CGAGGCTATA CCATCTCTGA TTCAGCCCCA TCACGAAGGG GCAAGAGGGT GGAGAGGGAT 
ACCTTCAAGA GCAGAGCTGC GGCCTCCAGT GCTGAGAAAG TGCCAGGCAT TAACCCTAGC 
TTTGTGTTCC TACAGCTCTA CCACTCACCC TTTTTTGGTG ATGAGTCCAA TAAGCCCATC 
CTGCTGCCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTCC TTGACCAGAT CCCGTCCTAT 
GACACCCACA AAATTGCTGT CCTGTATGTG GGAGAAGGCC AGAGCAACAG TGAGCTGGCC 
ATCCTGTCAA ATGAGCACGG CTCCTACAGG TACACGGAGT TTCTGACAGG CCTGGGCCGG 
CTCATCGAGC TCAAGGACTG CCAGCCAGAC AAGGTGTACT TGGGTGGACT AGATGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC TGTTTTCCAC 
ATTGCCACCC TGATGCCCAC CAAGGATGTG GACAAGCACC GCTGTGACAA GAAACGGCAC 
CTGGGCAATG ATTTTGTTTC TATCATCTAC AATGATTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCAAGG GCCAATTCAA CTTTGTCCAT GTGATCATCA CACCGCTGGA CTACAAATGC 
AACCTGTTGA CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAAATCGTAT CTGACCGAAA CCTGTCCTTC GTGGCCCGAC AGATGGCCCT ACATGCAAAT 
ATGGCCTCTC AGGTACACCA TAGCCGCTCC AACCCGACTG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT TAAGCGTCTC CGCCACCGGA TCCGTGAAGA AGTGCACTAT 
TCAAACCCAA GCTTGCCTCT GATGCACCCT CCAGCCCACA CCAAAGCCCC AGTTCAGGCC 
TCTGCTGAGC CCACACCCAC CTACGAGACA GGCCAGCGGA AACGCCTCAT CTCCTCCGTG 
GATGATTTCA CAGAGTTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005349218.1
CDS	119..5542
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSAPVKELRESCAAEAFRCRSVSVSEHVVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005349161.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005349161.2

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCTGC CGTGGTTCTC 
GAGGAAGGAA GTCCAGGAGA GGCTCATGTA CCAGTGGAGC CCCCCGAGTT GGAGGATTTT 
GAGGCAGTAG GTACAGACAG GCACTGCCAG CGTCCTGACA CCTACAGCAG GTCGTCCTCA 
GCGTCTAGCC AGGAAGAAAA GTCCCAGCTG GAGGAGATGG CTGCCGGAGG CATCCCCATC 
GAGCGGGCCA TCTCCGAGGG TGCTCGGCCT GCTGTGGACC TCTCCTTCCA ACCCTCACAG 
CCCCTGAGCA AGTCTAGCTC TTCTCCTGAG TTGCAGACCT TGCAAGACAT CCTCGGAGAC 
CTAGGGGACA AGGCTGACAT TGGACGGCTG AGTCCTGAGT CTAAGGTCCG GTCCCAGTCA 
GGGATCCTGG ATGGGGAAGT GGCTACCTGG TCAGCCCCAG GTGAAGAAGG CCGTGTCACA 
GTCCCACCTG AAGGTCCCCT GCCTTCCAGT TCTCCCCGCT CTCCTAGTGG TCTCCGGCCC 
CGAGGCTATA CCATCTCTGA TTCAGCCCCA TCACGAAGGG GCAAGAGGGT GGAGAGGGAT 
ACCTTCAAGA GCAGAGCTGC GGCCTCCAGT GCTGAGAAAG TGCCAGGCAT TAACCCTAGC 
TTTGTGTTCC TACAGCTCTA CCACTCACCC TTTTTTGGTG ATGAGTCCAA TAAGCCCATC 
CTGCTGCCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTCC TTGACCAGAT CCCGTCCTAT 
GACACCCACA AAATTGCTGT CCTGTATGTG GGAGAAGGCC AGAGCAACAG TGAGCTGGCC 
ATCCTGTCAA ATGAGCACGG CTCCTACAGG TACACGGAGT TTCTGACAGG CCTGGGCCGG 
CTCATCGAGC TCAAGGACTG CCAGCCAGAC AAGGTGTACT TGGGTGGACT AGATGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC TGTTTTCCAC 
ATTGCCACCC TGATGCCCAC CAAGGATGTG GACAAGCACC GCTGTGACAA GAAACGGCAC 
CTGGGCAATG ATTTTGTTTC TATCATCTAC AATGATTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCAAGG GCCAATTCAA CTTTGTCCAT GTGATCATCA CACCGCTGGA CTACAAATGC 
AACCTGTTGA CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAAATCGTAT CTGACCGAAA CCTGTCCTTC GTGGCCCGAC AGATGGCCCT ACATGCAAAT 
ATGGCCTCTC AGGTACACCA TAGCCGCTCC AACCCGACTG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT TAAGCGTCTC CGCCACCGGA TCCGTGAAGA AGTGCACTAT 
TCAAACCCAA GCTTGCCTCT GATGCACCCT CCAGCCCACA CCAAAGCCCC AGTTCAGGCC 
TCTGCTGAGC CCACACCCAC CTACGAGACA GGCCAGCGGA AACGCCTCAT CTCCTCCGTG 
GATGATTTCA CAGAGTTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_005349161.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_005349218.1 Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

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CloneID	OMi104122
Clone ID Related Accession (Same CDS sequence)	XM_005349161.2 , XM_005349161.1
Accession Version	XM_005349161.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004948990.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCTGC CGTGGTTCTC 
GAGGAAGGAA GTCCAGGAGA GGCTCATGTA CCAGTGGAGC CCCCCGAGTT GGAGGATTTT 
GAGGCAGTAG GTACAGACAG GCACTGCCAG CGTCCTGACA CCTACAGCAG GTCGTCCTCA 
GCGTCTAGCC AGGAAGAAAA GTCCCAGCTG GAGGAGATGG CTGCCGGAGG CATCCCCATC 
GAGCGGGCCA TCTCCGAGGG TGCTCGGCCT GCTGTGGACC TCTCCTTCCA ACCCTCACAG 
CCCCTGAGCA AGTCTAGCTC TTCTCCTGAG TTGCAGACCT TGCAAGACAT CCTCGGAGAC 
CTAGGGGACA AGGCTGACAT TGGACGGCTG AGTCCTGAGT CTAAGGTCCG GTCCCAGTCA 
GGGATCCTGG ATGGGGAAGT GGCTACCTGG TCAGCCCCAG GTGAAGAAGG CCGTGTCACA 
GTCCCACCTG AAGGTCCCCT GCCTTCCAGT TCTCCCCGCT CTCCTAGTGG TCTCCGGCCC 
CGAGGCTATA CCATCTCTGA TTCAGCCCCA TCACGAAGGG GCAAGAGGGT GGAGAGGGAT 
ACCTTCAAGA GCAGAGCTGC GGCCTCCAGT GCTGAGAAAG TGCCAGGCAT TAACCCTAGC 
TTTGTGTTCC TACAGCTCTA CCACTCACCC TTTTTTGGTG ATGAGTCCAA TAAGCCCATC 
CTGCTGCCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTCC TTGACCAGAT CCCGTCCTAT 
GACACCCACA AAATTGCTGT CCTGTATGTG GGAGAAGGCC AGAGCAACAG TGAGCTGGCC 
ATCCTGTCAA ATGAGCACGG CTCCTACAGG TACACGGAGT TTCTGACAGG CCTGGGCCGG 
CTCATCGAGC TCAAGGACTG CCAGCCAGAC AAGGTGTACT TGGGTGGACT AGATGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC TGTTTTCCAC 
ATTGCCACCC TGATGCCCAC CAAGGATGTG GACAAGCACC GCTGTGACAA GAAACGGCAC 
CTGGGCAATG ATTTTGTTTC TATCATCTAC AATGATTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCAAGG GCCAATTCAA CTTTGTCCAT GTGATCATCA CACCGCTGGA CTACAAATGC 
AACCTGTTGA CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAAATCGTAT CTGACCGAAA CCTGTCCTTC GTGGCCCGAC AGATGGCCCT ACATGCAAAT 
ATGGCCTCTC AGGTACACCA TAGCCGCTCC AACCCGACTG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT TAAGCGTCTC CGCCACCGGA TCCGTGAAGA AGTGCACTAT 
TCAAACCCAA GCTTGCCTCT GATGCACCCT CCAGCCCACA CCAAAGCCCC AGTTCAGGCC 
TCTGCTGAGC CCACACCCAC CTACGAGACA GGCCAGCGGA AACGCCTCAT CTCCTCCGTG 
GATGATTTCA CAGAGTTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005349218.1
CDS	100..5523
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSAPVKELRESCAAEAFRCRSVSVSEHVVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005349161.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005349161.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCTGC CGTGGTTCTC 
GAGGAAGGAA GTCCAGGAGA GGCTCATGTA CCAGTGGAGC CCCCCGAGTT GGAGGATTTT 
GAGGCAGTAG GTACAGACAG GCACTGCCAG CGTCCTGACA CCTACAGCAG GTCGTCCTCA 
GCGTCTAGCC AGGAAGAAAA GTCCCAGCTG GAGGAGATGG CTGCCGGAGG CATCCCCATC 
GAGCGGGCCA TCTCCGAGGG TGCTCGGCCT GCTGTGGACC TCTCCTTCCA ACCCTCACAG 
CCCCTGAGCA AGTCTAGCTC TTCTCCTGAG TTGCAGACCT TGCAAGACAT CCTCGGAGAC 
CTAGGGGACA AGGCTGACAT TGGACGGCTG AGTCCTGAGT CTAAGGTCCG GTCCCAGTCA 
GGGATCCTGG ATGGGGAAGT GGCTACCTGG TCAGCCCCAG GTGAAGAAGG CCGTGTCACA 
GTCCCACCTG AAGGTCCCCT GCCTTCCAGT TCTCCCCGCT CTCCTAGTGG TCTCCGGCCC 
CGAGGCTATA CCATCTCTGA TTCAGCCCCA TCACGAAGGG GCAAGAGGGT GGAGAGGGAT 
ACCTTCAAGA GCAGAGCTGC GGCCTCCAGT GCTGAGAAAG TGCCAGGCAT TAACCCTAGC 
TTTGTGTTCC TACAGCTCTA CCACTCACCC TTTTTTGGTG ATGAGTCCAA TAAGCCCATC 
CTGCTGCCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTCC TTGACCAGAT CCCGTCCTAT 
GACACCCACA AAATTGCTGT CCTGTATGTG GGAGAAGGCC AGAGCAACAG TGAGCTGGCC 
ATCCTGTCAA ATGAGCACGG CTCCTACAGG TACACGGAGT TTCTGACAGG CCTGGGCCGG 
CTCATCGAGC TCAAGGACTG CCAGCCAGAC AAGGTGTACT TGGGTGGACT AGATGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC TGTTTTCCAC 
ATTGCCACCC TGATGCCCAC CAAGGATGTG GACAAGCACC GCTGTGACAA GAAACGGCAC 
CTGGGCAATG ATTTTGTTTC TATCATCTAC AATGATTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCAAGG GCCAATTCAA CTTTGTCCAT GTGATCATCA CACCGCTGGA CTACAAATGC 
AACCTGTTGA CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAAATCGTAT CTGACCGAAA CCTGTCCTTC GTGGCCCGAC AGATGGCCCT ACATGCAAAT 
ATGGCCTCTC AGGTACACCA TAGCCGCTCC AACCCGACTG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT TAAGCGTCTC CGCCACCGGA TCCGTGAAGA AGTGCACTAT 
TCAAACCCAA GCTTGCCTCT GATGCACCCT CCAGCCCACA CCAAAGCCCC AGTTCAGGCC 
TCTGCTGAGC CCACACCCAC CTACGAGACA GGCCAGCGGA AACGCCTCAT CTCCTCCGTG 
GATGATTTCA CAGAGTTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347152.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202606.1 Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMi104123
Clone ID Related Accession (Same CDS sequence)	XM_013347152.1 , XM_013347152.2
Accession Version	XM_013347152.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004948990.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202606.1
CDS	111..5465
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSAPVKELRESCAAEAFRCRSVSVSEHVVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTDSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347152.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347152.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347152.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202606.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.

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CloneID	OMi104123
Clone ID Related Accession (Same CDS sequence)	XM_013347152.1 , XM_013347152.2
Accession Version	XM_013347152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_013347152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202606.1
CDS	119..5473
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSAPVKELRESCAAEAFRCRSVSVSEHVVRSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTDSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347152.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347152.2

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGA ATCCAGACAT CCCTCACCAG CGCTAGCCTG 
GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA GCTTGAAGAA TCTCCACTTG 
GAGCTCACAG AAACGTGTCT GGACATGATG GCCCGATACG TGTTCTCCAA CTTCACTGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCAG GGGGTCGGAC CAAGACCTGG 
CTGGTCGGAA ATAAGCTCGT CACCGTGACA ACCAGCGTGG GAACCGGCAC CCGGTCGTTG 
CTGGGCCTGG ATTCAGGGGA TCTGCAGGGT GGCCCAGAGC CAAGCTCTGA TCCTGGGGTA 
CATGTGAGGC AGACAAAGGA AGCACCAGCC AAACTGGAGT CGCAGTCTGG GCAGCAGGTG 
TCGCGTGGGG CCCGGGACCG GGTCCGCTCC ATGTCAGGGG GACATGGCCT TCGAGTTGGT 
GCCCTGGATA CTCCAGCTCC CCATACCCCA GGTGGCCCTG CTCCCCTGGG ACCACAGACG 
GCACCAGCAG CAAAGCCTGA GAAGGCTTCT GCGGGAACCC AGCTTCCGAA AGCAGAGAAG 
ACGAATTTGG CAGCTTATGT ACCTTTGCTG ACCCAGGGCT GGGCTGAAAT TTTGGTCCGT 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCGTTCTCC 
TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAATG CTCTTATGGC TGCCGAGCGA 
TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCCCTGT CAGTGCCAGC GGCTGGCACA 
GCCAAGCCAC CTCCTCTTCC ACGCTCTAAC ACAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347153.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202607.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.

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CloneID	OMi104124
Clone ID Related Accession (Same CDS sequence)	XM_013347153.2 , XM_013347153.1
Accession Version	XM_013347153.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_013347153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGTGGCT TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202607.1
CDS	119..5413
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347153.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347153.2

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGTGGCT TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347153.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202607.1 Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

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CloneID	OMi104124
Clone ID Related Accession (Same CDS sequence)	XM_013347153.2 , XM_013347153.1
Accession Version	XM_013347153.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004948990.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGTGGCT TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202607.1
CDS	100..5394
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347153.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347153.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGTGGCT TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCTG CCGTGGTTCT CGAGGAAGGA 
AGTCCAGGAG AGGCTCATGT ACCAGTGGAG CCCCCCGAGT TGGAGGATTT TGAGGCAGTA 
GGTACAGACA GGCACTGCCA GCGTCCTGAC ACCTACAGCA GGTCGTCCTC AGCGTCTAGC 
CAGGAAGAAA AGTCCCAGCT GGAGGAGATG GCTGCCGGAG GCATCCCCAT CGAGCGGGCC 
ATCTCCGAGG GTGCTCGGCC TGCTGTGGAC CTCTCCTTCC AACCCTCACA GCCCCTGAGC 
AAGTCTAGCT CTTCTCCTGA GTTGCAGACC TTGCAAGACA TCCTCGGAGA CCTAGGGGAC 
AAGGCTGACA TTGGACGGCT GAGTCCTGAG TCTAAGGTCC GGTCCCAGTC AGGGATCCTG 
GATGGGGAAG TGGCTACCTG GTCAGCCCCA GGTGAAGAAG GCCGTGTCAC AGTCCCACCT 
GAAGGTCCCC TGCCTTCCAG TTCTCCCCGC TCTCCTAGTG GTCTCCGGCC CCGAGGCTAT 
ACCATCTCTG ATTCAGCCCC ATCACGAAGG GGCAAGAGGG TGGAGAGGGA TACCTTCAAG 
AGCAGAGCTG CGGCCTCCAG TGCTGAGAAA GTGCCAGGCA TTAACCCTAG CTTTGTGTTC 
CTACAGCTCT ACCACTCACC CTTTTTTGGT GATGAGTCCA ATAAGCCCAT CCTGCTGCCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTC CTTGACCAGA TCCCGTCCTA TGACACCCAC 
AAAATTGCTG TCCTGTATGT GGGAGAAGGC CAGAGCAACA GTGAGCTGGC CATCCTGTCA 
AATGAGCACG GCTCCTACAG GTACACGGAG TTTCTGACAG GCCTGGGCCG GCTCATCGAG 
CTCAAGGACT GCCAGCCAGA CAAGGTGTAC TTGGGTGGAC TAGATGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CTGTTTTCCA CATTGCCACC 
CTGATGCCCA CCAAGGATGT GGACAAGCAC CGCTGTGACA AGAAACGGCA CCTGGGCAAT 
GATTTTGTTT CTATCATCTA CAATGATTCT GGTGAGGACT TCAAGCTGGG CACCATCAAG 
GGCCAATTCA ACTTTGTCCA TGTGATCATC ACACCGCTGG ACTACAAATG CAACCTGTTG 
ACCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAAATCGTA 
TCTGACCGAA ACCTGTCCTT CGTGGCCCGA CAGATGGCCC TACATGCAAA TATGGCCTCT 
CAGGTACACC ATAGCCGCTC CAACCCGACT GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TTAAGCGTCT CCGCCACCGG ATCCGTGAAG AAGTGCACTA TTCAAACCCA 
AGCTTGCCTC TGATGCACCC TCCAGCCCAC ACCAAAGCCC CAGTTCAGGC CTCTGCTGAG 
CCCACACCCA CCTACGAGAC AGGCCAGCGG AAACGCCTCA TCTCCTCCGT GGATGATTTC 
ACAGAGTTTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347154.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202608.1 Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

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CloneID	OMi104125
Clone ID Related Accession (Same CDS sequence)	XM_013347154.1 , XM_013347154.2
Accession Version	XM_013347154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004948990.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGTGGCTTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCTGCCG TGGTTCTCGA GGAAGGAAGT 
CCAGGAGAGG CTCATGTACC AGTGGAGCCC CCCGAGTTGG AGGATTTTGA GGCAGTAGGT 
ACAGACAGGC ACTGCCAGCG TCCTGACACC TACAGCAGGT CGTCCTCAGC GTCTAGCCAG 
GAAGAAAAGT CCCAGCTGGA GGAGATGGCT GCCGGAGGCA TCCCCATCGA GCGGGCCATC 
TCCGAGGGTG CTCGGCCTGC TGTGGACCTC TCCTTCCAAC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCTT CTCCTGAGTT GCAGACCTTG CAAGACATCC TCGGAGACCT AGGGGACAAG 
GCTGACATTG GACGGCTGAG TCCTGAGTCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGTGG CTACCTGGTC AGCCCCAGGT GAAGAAGGCC GTGTCACAGT CCCACCTGAA 
GGTCCCCTGC CTTCCAGTTC TCCCCGCTCT CCTAGTGGTC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CAGCCCCATC ACGAAGGGGC AAGAGGGTGG AGAGGGATAC CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAAGTG CCAGGCATTA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ACTCACCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT GCTGCCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAGCTCCTT GACCAGATCC CGTCCTATGA CACCCACAAA 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAACAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCACGGCT CCTACAGGTA CACGGAGTTT CTGACAGGCC TGGGCCGGCT CATCGAGCTC 
AAGGACTGCC AGCCAGACAA GGTGTACTTG GGTGGACTAG ATGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAT 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AGCTGGGCAC CATCAAGGGC 
CAATTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAATGCAA CCTGTTGACC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA AATCGTATCT 
GACCGAAACC TGTCCTTCGT GGCCCGACAG ATGGCCCTAC ATGCAAATAT GGCCTCTCAG 
GTACACCATA GCCGCTCCAA CCCGACTGAC ATCTATCCCT CCAAGTGGAT TGCCAGGCTT 
CGCCACATTA AGCGTCTCCG CCACCGGATC CGTGAAGAAG TGCACTATTC AAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG TTCAGGCCTC TGCTGAGCCC 
ACACCCACCT ACGAGACAGG CCAGCGGAAA CGCCTCATCT CCTCCGTGGA TGATTTCACA 
GAGTTTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202608.1
CDS	100..5391
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347154.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347154.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGTGGCTTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCTGCCG TGGTTCTCGA GGAAGGAAGT 
CCAGGAGAGG CTCATGTACC AGTGGAGCCC CCCGAGTTGG AGGATTTTGA GGCAGTAGGT 
ACAGACAGGC ACTGCCAGCG TCCTGACACC TACAGCAGGT CGTCCTCAGC GTCTAGCCAG 
GAAGAAAAGT CCCAGCTGGA GGAGATGGCT GCCGGAGGCA TCCCCATCGA GCGGGCCATC 
TCCGAGGGTG CTCGGCCTGC TGTGGACCTC TCCTTCCAAC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCTT CTCCTGAGTT GCAGACCTTG CAAGACATCC TCGGAGACCT AGGGGACAAG 
GCTGACATTG GACGGCTGAG TCCTGAGTCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGTGG CTACCTGGTC AGCCCCAGGT GAAGAAGGCC GTGTCACAGT CCCACCTGAA 
GGTCCCCTGC CTTCCAGTTC TCCCCGCTCT CCTAGTGGTC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CAGCCCCATC ACGAAGGGGC AAGAGGGTGG AGAGGGATAC CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAAGTG CCAGGCATTA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ACTCACCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT GCTGCCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAGCTCCTT GACCAGATCC CGTCCTATGA CACCCACAAA 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAACAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCACGGCT CCTACAGGTA CACGGAGTTT CTGACAGGCC TGGGCCGGCT CATCGAGCTC 
AAGGACTGCC AGCCAGACAA GGTGTACTTG GGTGGACTAG ATGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAT 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AGCTGGGCAC CATCAAGGGC 
CAATTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAATGCAA CCTGTTGACC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA AATCGTATCT 
GACCGAAACC TGTCCTTCGT GGCCCGACAG ATGGCCCTAC ATGCAAATAT GGCCTCTCAG 
GTACACCATA GCCGCTCCAA CCCGACTGAC ATCTATCCCT CCAAGTGGAT TGCCAGGCTT 
CGCCACATTA AGCGTCTCCG CCACCGGATC CGTGAAGAAG TGCACTATTC AAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG TTCAGGCCTC TGCTGAGCCC 
ACACCCACCT ACGAGACAGG CCAGCGGAAA CGCCTCATCT CCTCCGTGGA TGATTTCACA 
GAGTTTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347154.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202608.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.

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CloneID	OMi104125
Clone ID Related Accession (Same CDS sequence)	XM_013347154.1 , XM_013347154.2
Accession Version	XM_013347154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_013347154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGTGGCTTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCTGCCG TGGTTCTCGA GGAAGGAAGT 
CCAGGAGAGG CTCATGTACC AGTGGAGCCC CCCGAGTTGG AGGATTTTGA GGCAGTAGGT 
ACAGACAGGC ACTGCCAGCG TCCTGACACC TACAGCAGGT CGTCCTCAGC GTCTAGCCAG 
GAAGAAAAGT CCCAGCTGGA GGAGATGGCT GCCGGAGGCA TCCCCATCGA GCGGGCCATC 
TCCGAGGGTG CTCGGCCTGC TGTGGACCTC TCCTTCCAAC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCTT CTCCTGAGTT GCAGACCTTG CAAGACATCC TCGGAGACCT AGGGGACAAG 
GCTGACATTG GACGGCTGAG TCCTGAGTCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGTGG CTACCTGGTC AGCCCCAGGT GAAGAAGGCC GTGTCACAGT CCCACCTGAA 
GGTCCCCTGC CTTCCAGTTC TCCCCGCTCT CCTAGTGGTC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CAGCCCCATC ACGAAGGGGC AAGAGGGTGG AGAGGGATAC CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAAGTG CCAGGCATTA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ACTCACCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT GCTGCCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAGCTCCTT GACCAGATCC CGTCCTATGA CACCCACAAA 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAACAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCACGGCT CCTACAGGTA CACGGAGTTT CTGACAGGCC TGGGCCGGCT CATCGAGCTC 
AAGGACTGCC AGCCAGACAA GGTGTACTTG GGTGGACTAG ATGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAT 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AGCTGGGCAC CATCAAGGGC 
CAATTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAATGCAA CCTGTTGACC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA AATCGTATCT 
GACCGAAACC TGTCCTTCGT GGCCCGACAG ATGGCCCTAC ATGCAAATAT GGCCTCTCAG 
GTACACCATA GCCGCTCCAA CCCGACTGAC ATCTATCCCT CCAAGTGGAT TGCCAGGCTT 
CGCCACATTA AGCGTCTCCG CCACCGGATC CGTGAAGAAG TGCACTATTC AAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG TTCAGGCCTC TGCTGAGCCC 
ACACCCACCT ACGAGACAGG CCAGCGGAAA CGCCTCATCT CCTCCGTGGA TGATTTCACA 
GAGTTTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202608.1
CDS	119..5410
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347154.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347154.2

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGTGGCTTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCTGCCG TGGTTCTCGA GGAAGGAAGT 
CCAGGAGAGG CTCATGTACC AGTGGAGCCC CCCGAGTTGG AGGATTTTGA GGCAGTAGGT 
ACAGACAGGC ACTGCCAGCG TCCTGACACC TACAGCAGGT CGTCCTCAGC GTCTAGCCAG 
GAAGAAAAGT CCCAGCTGGA GGAGATGGCT GCCGGAGGCA TCCCCATCGA GCGGGCCATC 
TCCGAGGGTG CTCGGCCTGC TGTGGACCTC TCCTTCCAAC CCTCACAGCC CCTGAGCAAG 
TCTAGCTCTT CTCCTGAGTT GCAGACCTTG CAAGACATCC TCGGAGACCT AGGGGACAAG 
GCTGACATTG GACGGCTGAG TCCTGAGTCT AAGGTCCGGT CCCAGTCAGG GATCCTGGAT 
GGGGAAGTGG CTACCTGGTC AGCCCCAGGT GAAGAAGGCC GTGTCACAGT CCCACCTGAA 
GGTCCCCTGC CTTCCAGTTC TCCCCGCTCT CCTAGTGGTC TCCGGCCCCG AGGCTATACC 
ATCTCTGATT CAGCCCCATC ACGAAGGGGC AAGAGGGTGG AGAGGGATAC CTTCAAGAGC 
AGAGCTGCGG CCTCCAGTGC TGAGAAAGTG CCAGGCATTA ACCCTAGCTT TGTGTTCCTA 
CAGCTCTACC ACTCACCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT GCTGCCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAGCTCCTT GACCAGATCC CGTCCTATGA CACCCACAAA 
ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAACAGTG AGCTGGCCAT CCTGTCAAAT 
GAGCACGGCT CCTACAGGTA CACGGAGTTT CTGACAGGCC TGGGCCGGCT CATCGAGCTC 
AAGGACTGCC AGCCAGACAA GGTGTACTTG GGTGGACTAG ATGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCTG TTTTCCACAT TGCCACCCTG 
ATGCCCACCA AGGATGTGGA CAAGCACCGC TGTGACAAGA AACGGCACCT GGGCAATGAT 
TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AGCTGGGCAC CATCAAGGGC 
CAATTCAACT TTGTCCATGT GATCATCACA CCGCTGGACT ACAAATGCAA CCTGTTGACC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA AATCGTATCT 
GACCGAAACC TGTCCTTCGT GGCCCGACAG ATGGCCCTAC ATGCAAATAT GGCCTCTCAG 
GTACACCATA GCCGCTCCAA CCCGACTGAC ATCTATCCCT CCAAGTGGAT TGCCAGGCTT 
CGCCACATTA AGCGTCTCCG CCACCGGATC CGTGAAGAAG TGCACTATTC AAACCCAAGC 
TTGCCTCTGA TGCACCCTCC AGCCCACACC AAAGCCCCAG TTCAGGCCTC TGCTGAGCCC 
ACACCCACCT ACGAGACAGG CCAGCGGAAA CGCCTCATCT CCTCCGTGGA TGATTTCACA 
GAGTTTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347155.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202609.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.

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CloneID	OMi104126
Clone ID Related Accession (Same CDS sequence)	XM_013347155.2 , XM_013347155.1
Accession Version	XM_013347155.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_013347155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGACTCT GCCGTGGTTC TCGAGGAAGG AAGTCCAGGA 
GAGGCTCATG TACCAGTGGA GCCCCCCGAG TTGGAGGATT TTGAGGCAGT AGGTACAGAC 
AGGCACTGCC AGCGTCCTGA CACCTACAGC AGGTCGTCCT CAGCGTCTAG CCAGGAAGAA 
AAGTCCCAGC TGGAGGAGAT GGCTGCCGGA GGCATCCCCA TCGAGCGGGC CATCTCCGAG 
GGTGCTCGGC CTGCTGTGGA CCTCTCCTTC CAACCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCTTCTCCTG AGTTGCAGAC CTTGCAAGAC ATCCTCGGAG ACCTAGGGGA CAAGGCTGAC 
ATTGGACGGC TGAGTCCTGA GTCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GTGGCTACCT GGTCAGCCCC AGGTGAAGAA GGCCGTGTCA CAGTCCCACC TGAAGGTCCC 
CTGCCTTCCA GTTCTCCCCG CTCTCCTAGT GGTCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCAGCCC CATCACGAAG GGGCAAGAGG GTGGAGAGGG ATACCTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA AGTGCCAGGC ATTAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCACTCAC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTGCTGCC CAATGAGTCC 
TTCGAGCGGT CAGTGCAGCT CCTTGACCAG ATCCCGTCCT ATGACACCCA CAAAATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTGG CCATCCTGTC AAATGAGCAC 
GGCTCCTACA GGTACACGGA GTTTCTGACA GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CTTGGGTGGA CTAGATGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGATTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAGCTGG GCACCATCAA GGGCCAATTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAAT GCAACCTGTT GACCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAAATCGT ATCTGACCGA 
AACCTGTCCT TCGTGGCCCG ACAGATGGCC CTACATGCAA ATATGGCCTC TCAGGTACAC 
CATAGCCGCT CCAACCCGAC TGACATCTAT CCCTCCAAGT GGATTGCCAG GCTTCGCCAC 
ATTAAGCGTC TCCGCCACCG GATCCGTGAA GAAGTGCACT ATTCAAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGTTCAGG CCTCTGCTGA GCCCACACCC 
ACCTACGAGA CAGGCCAGCG GAAACGCCTC ATCTCCTCCG TGGATGATTT CACAGAGTTT 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202609.1
CDS	119..5344
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTDSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347155.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347155.2

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGACTCT GCCGTGGTTC TCGAGGAAGG AAGTCCAGGA 
GAGGCTCATG TACCAGTGGA GCCCCCCGAG TTGGAGGATT TTGAGGCAGT AGGTACAGAC 
AGGCACTGCC AGCGTCCTGA CACCTACAGC AGGTCGTCCT CAGCGTCTAG CCAGGAAGAA 
AAGTCCCAGC TGGAGGAGAT GGCTGCCGGA GGCATCCCCA TCGAGCGGGC CATCTCCGAG 
GGTGCTCGGC CTGCTGTGGA CCTCTCCTTC CAACCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCTTCTCCTG AGTTGCAGAC CTTGCAAGAC ATCCTCGGAG ACCTAGGGGA CAAGGCTGAC 
ATTGGACGGC TGAGTCCTGA GTCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GTGGCTACCT GGTCAGCCCC AGGTGAAGAA GGCCGTGTCA CAGTCCCACC TGAAGGTCCC 
CTGCCTTCCA GTTCTCCCCG CTCTCCTAGT GGTCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCAGCCC CATCACGAAG GGGCAAGAGG GTGGAGAGGG ATACCTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA AGTGCCAGGC ATTAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCACTCAC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTGCTGCC CAATGAGTCC 
TTCGAGCGGT CAGTGCAGCT CCTTGACCAG ATCCCGTCCT ATGACACCCA CAAAATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTGG CCATCCTGTC AAATGAGCAC 
GGCTCCTACA GGTACACGGA GTTTCTGACA GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CTTGGGTGGA CTAGATGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGATTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAGCTGG GCACCATCAA GGGCCAATTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAAT GCAACCTGTT GACCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAAATCGT ATCTGACCGA 
AACCTGTCCT TCGTGGCCCG ACAGATGGCC CTACATGCAA ATATGGCCTC TCAGGTACAC 
CATAGCCGCT CCAACCCGAC TGACATCTAT CCCTCCAAGT GGATTGCCAG GCTTCGCCAC 
ATTAAGCGTC TCCGCCACCG GATCCGTGAA GAAGTGCACT ATTCAAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGTTCAGG CCTCTGCTGA GCCCACACCC 
ACCTACGAGA CAGGCCAGCG GAAACGCCTC ATCTCCTCCG TGGATGATTT CACAGAGTTT 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347155.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202609.1 Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

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CloneID	OMi104126
Clone ID Related Accession (Same CDS sequence)	XM_013347155.2 , XM_013347155.1
Accession Version	XM_013347155.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004948990.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGACTCT GCCGTGGTTC TCGAGGAAGG AAGTCCAGGA 
GAGGCTCATG TACCAGTGGA GCCCCCCGAG TTGGAGGATT TTGAGGCAGT AGGTACAGAC 
AGGCACTGCC AGCGTCCTGA CACCTACAGC AGGTCGTCCT CAGCGTCTAG CCAGGAAGAA 
AAGTCCCAGC TGGAGGAGAT GGCTGCCGGA GGCATCCCCA TCGAGCGGGC CATCTCCGAG 
GGTGCTCGGC CTGCTGTGGA CCTCTCCTTC CAACCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCTTCTCCTG AGTTGCAGAC CTTGCAAGAC ATCCTCGGAG ACCTAGGGGA CAAGGCTGAC 
ATTGGACGGC TGAGTCCTGA GTCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GTGGCTACCT GGTCAGCCCC AGGTGAAGAA GGCCGTGTCA CAGTCCCACC TGAAGGTCCC 
CTGCCTTCCA GTTCTCCCCG CTCTCCTAGT GGTCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCAGCCC CATCACGAAG GGGCAAGAGG GTGGAGAGGG ATACCTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA AGTGCCAGGC ATTAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCACTCAC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTGCTGCC CAATGAGTCC 
TTCGAGCGGT CAGTGCAGCT CCTTGACCAG ATCCCGTCCT ATGACACCCA CAAAATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTGG CCATCCTGTC AAATGAGCAC 
GGCTCCTACA GGTACACGGA GTTTCTGACA GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CTTGGGTGGA CTAGATGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGATTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAGCTGG GCACCATCAA GGGCCAATTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAAT GCAACCTGTT GACCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAAATCGT ATCTGACCGA 
AACCTGTCCT TCGTGGCCCG ACAGATGGCC CTACATGCAA ATATGGCCTC TCAGGTACAC 
CATAGCCGCT CCAACCCGAC TGACATCTAT CCCTCCAAGT GGATTGCCAG GCTTCGCCAC 
ATTAAGCGTC TCCGCCACCG GATCCGTGAA GAAGTGCACT ATTCAAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGTTCAGG CCTCTGCTGA GCCCACACCC 
ACCTACGAGA CAGGCCAGCG GAAACGCCTC ATCTCCTCCG TGGATGATTT CACAGAGTTT 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202609.1
CDS	100..5325
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTDSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347155.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347155.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGCAG AATCCAGACA TCCCTCACCA GCGCTAGCCT GGGGTCTGCG 
GATGAGAACT CTATGGCCCA GGCTGATGAC AGCTTGAAGA ATCTCCACTT GGAGCTCACA 
GAAACGTGTC TGGACATGAT GGCCCGATAC GTGTTCTCCA ACTTCACTGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCA GGGGGTCGGA CCAAGACCTG GCTGGTCGGA 
AATAAGCTCG TCACCGTGAC AACCAGCGTG GGAACCGGCA CCCGGTCGTT GCTGGGCCTG 
GATTCAGGGG ATCTGCAGGG TGGCCCAGAG CCAAGCTCTG ATCCTGGGGT ACATGTGAGG 
CAGACAAAGG AAGCACCAGC CAAACTGGAG TCGCAGTCTG GGCAGCAGGT GTCGCGTGGG 
GCCCGGGACC GGGTCCGCTC CATGTCAGGG GGACATGGCC TTCGAGTTGG TGCCCTGGAT 
ACTCCAGCTC CCCATACCCC AGGTGGCCCT GCTCCCCTGG GACCACAGAC GGCACCAGCA 
GCAAAGCCTG AGAAGGCTTC TGCGGGAACC CAGCTTCCGA AAGCAGAGAA GACGAATTTG 
GCAGCTTATG TACCTTTGCT GACCCAGGGC TGGGCTGAAA TTTTGGTCCG TAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCGTTCTC CTCAGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAT GCTCTTATGG CTGCCGAGCG ATTCAAGGAG 
CACCGGGACA CGGCCCTGTA CAAGTCCCTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCA 
CCTCCTCTTC CACGCTCTAA CACAGACTCT GCCGTGGTTC TCGAGGAAGG AAGTCCAGGA 
GAGGCTCATG TACCAGTGGA GCCCCCCGAG TTGGAGGATT TTGAGGCAGT AGGTACAGAC 
AGGCACTGCC AGCGTCCTGA CACCTACAGC AGGTCGTCCT CAGCGTCTAG CCAGGAAGAA 
AAGTCCCAGC TGGAGGAGAT GGCTGCCGGA GGCATCCCCA TCGAGCGGGC CATCTCCGAG 
GGTGCTCGGC CTGCTGTGGA CCTCTCCTTC CAACCCTCAC AGCCCCTGAG CAAGTCTAGC 
TCTTCTCCTG AGTTGCAGAC CTTGCAAGAC ATCCTCGGAG ACCTAGGGGA CAAGGCTGAC 
ATTGGACGGC TGAGTCCTGA GTCTAAGGTC CGGTCCCAGT CAGGGATCCT GGATGGGGAA 
GTGGCTACCT GGTCAGCCCC AGGTGAAGAA GGCCGTGTCA CAGTCCCACC TGAAGGTCCC 
CTGCCTTCCA GTTCTCCCCG CTCTCCTAGT GGTCTCCGGC CCCGAGGCTA TACCATCTCT 
GATTCAGCCC CATCACGAAG GGGCAAGAGG GTGGAGAGGG ATACCTTCAA GAGCAGAGCT 
GCGGCCTCCA GTGCTGAGAA AGTGCCAGGC ATTAACCCTA GCTTTGTGTT CCTACAGCTC 
TACCACTCAC CCTTTTTTGG TGATGAGTCC AATAAGCCCA TCCTGCTGCC CAATGAGTCC 
TTCGAGCGGT CAGTGCAGCT CCTTGACCAG ATCCCGTCCT ATGACACCCA CAAAATTGCT 
GTCCTGTATG TGGGAGAAGG CCAGAGCAAC AGTGAGCTGG CCATCCTGTC AAATGAGCAC 
GGCTCCTACA GGTACACGGA GTTTCTGACA GGCCTGGGCC GGCTCATCGA GCTCAAGGAC 
TGCCAGCCAG ACAAGGTGTA CTTGGGTGGA CTAGATGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCTGTTTTCC ACATTGCCAC CCTGATGCCC 
ACCAAGGATG TGGACAAGCA CCGCTGTGAC AAGAAACGGC ACCTGGGCAA TGATTTTGTT 
TCTATCATCT ACAATGATTC TGGTGAGGAC TTCAAGCTGG GCACCATCAA GGGCCAATTC 
AACTTTGTCC ATGTGATCAT CACACCGCTG GACTACAAAT GCAACCTGTT GACCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAAATCGT ATCTGACCGA 
AACCTGTCCT TCGTGGCCCG ACAGATGGCC CTACATGCAA ATATGGCCTC TCAGGTACAC 
CATAGCCGCT CCAACCCGAC TGACATCTAT CCCTCCAAGT GGATTGCCAG GCTTCGCCAC 
ATTAAGCGTC TCCGCCACCG GATCCGTGAA GAAGTGCACT ATTCAAACCC AAGCTTGCCT 
CTGATGCACC CTCCAGCCCA CACCAAAGCC CCAGTTCAGG CCTCTGCTGA GCCCACACCC 
ACCTACGAGA CAGGCCAGCG GAAACGCCTC ATCTCCTCCG TGGATGATTT CACAGAGTTT 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347156.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202610.1 Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

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CloneID	OMi104127
Clone ID Related Accession (Same CDS sequence)	XM_013347156.1 , XM_013347156.2
Accession Version	XM_013347156.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-06
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004948990.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microtus ochrogaster Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGACTCTGCC GTGGTTCTCG AGGAAGGAAG TCCAGGAGAG 
GCTCATGTAC CAGTGGAGCC CCCCGAGTTG GAGGATTTTG AGGCAGTAGG TACAGACAGG 
CACTGCCAGC GTCCTGACAC CTACAGCAGG TCGTCCTCAG CGTCTAGCCA GGAAGAAAAG 
TCCCAGCTGG AGGAGATGGC TGCCGGAGGC ATCCCCATCG AGCGGGCCAT CTCCGAGGGT 
GCTCGGCCTG CTGTGGACCT CTCCTTCCAA CCCTCACAGC CCCTGAGCAA GTCTAGCTCT 
TCTCCTGAGT TGCAGACCTT GCAAGACATC CTCGGAGACC TAGGGGACAA GGCTGACATT 
GGACGGCTGA GTCCTGAGTC TAAGGTCCGG TCCCAGTCAG GGATCCTGGA TGGGGAAGTG 
GCTACCTGGT CAGCCCCAGG TGAAGAAGGC CGTGTCACAG TCCCACCTGA AGGTCCCCTG 
CCTTCCAGTT CTCCCCGCTC TCCTAGTGGT CTCCGGCCCC GAGGCTATAC CATCTCTGAT 
TCAGCCCCAT CACGAAGGGG CAAGAGGGTG GAGAGGGATA CCTTCAAGAG CAGAGCTGCG 
GCCTCCAGTG CTGAGAAAGT GCCAGGCATT AACCCTAGCT TTGTGTTCCT ACAGCTCTAC 
CACTCACCCT TTTTTGGTGA TGAGTCCAAT AAGCCCATCC TGCTGCCCAA TGAGTCCTTC 
GAGCGGTCAG TGCAGCTCCT TGACCAGATC CCGTCCTATG ACACCCACAA AATTGCTGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTGGCCA TCCTGTCAAA TGAGCACGGC 
TCCTACAGGT ACACGGAGTT TCTGACAGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACTT GGGTGGACTA GATGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCT GTTTTCCACA TTGCCACCCT GATGCCCACC 
AAGGATGTGG ACAAGCACCG CTGTGACAAG AAACGGCACC TGGGCAATGA TTTTGTTTCT 
ATCATCTACA ATGATTCTGG TGAGGACTTC AAGCTGGGCA CCATCAAGGG CCAATTCAAC 
TTTGTCCATG TGATCATCAC ACCGCTGGAC TACAAATGCA ACCTGTTGAC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AAATCGTATC TGACCGAAAC 
CTGTCCTTCG TGGCCCGACA GATGGCCCTA CATGCAAATA TGGCCTCTCA GGTACACCAT 
AGCCGCTCCA ACCCGACTGA CATCTATCCC TCCAAGTGGA TTGCCAGGCT TCGCCACATT 
AAGCGTCTCC GCCACCGGAT CCGTGAAGAA GTGCACTATT CAAACCCAAG CTTGCCTCTG 
ATGCACCCTC CAGCCCACAC CAAAGCCCCA GTTCAGGCCT CTGCTGAGCC CACACCCACC 
TACGAGACAG GCCAGCGGAA ACGCCTCATC TCCTCCGTGG ATGATTTCAC AGAGTTTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202610.1
CDS	100..5322
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTDSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347156.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347156.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGACTCTGCC GTGGTTCTCG AGGAAGGAAG TCCAGGAGAG 
GCTCATGTAC CAGTGGAGCC CCCCGAGTTG GAGGATTTTG AGGCAGTAGG TACAGACAGG 
CACTGCCAGC GTCCTGACAC CTACAGCAGG TCGTCCTCAG CGTCTAGCCA GGAAGAAAAG 
TCCCAGCTGG AGGAGATGGC TGCCGGAGGC ATCCCCATCG AGCGGGCCAT CTCCGAGGGT 
GCTCGGCCTG CTGTGGACCT CTCCTTCCAA CCCTCACAGC CCCTGAGCAA GTCTAGCTCT 
TCTCCTGAGT TGCAGACCTT GCAAGACATC CTCGGAGACC TAGGGGACAA GGCTGACATT 
GGACGGCTGA GTCCTGAGTC TAAGGTCCGG TCCCAGTCAG GGATCCTGGA TGGGGAAGTG 
GCTACCTGGT CAGCCCCAGG TGAAGAAGGC CGTGTCACAG TCCCACCTGA AGGTCCCCTG 
CCTTCCAGTT CTCCCCGCTC TCCTAGTGGT CTCCGGCCCC GAGGCTATAC CATCTCTGAT 
TCAGCCCCAT CACGAAGGGG CAAGAGGGTG GAGAGGGATA CCTTCAAGAG CAGAGCTGCG 
GCCTCCAGTG CTGAGAAAGT GCCAGGCATT AACCCTAGCT TTGTGTTCCT ACAGCTCTAC 
CACTCACCCT TTTTTGGTGA TGAGTCCAAT AAGCCCATCC TGCTGCCCAA TGAGTCCTTC 
GAGCGGTCAG TGCAGCTCCT TGACCAGATC CCGTCCTATG ACACCCACAA AATTGCTGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTGGCCA TCCTGTCAAA TGAGCACGGC 
TCCTACAGGT ACACGGAGTT TCTGACAGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACTT GGGTGGACTA GATGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCT GTTTTCCACA TTGCCACCCT GATGCCCACC 
AAGGATGTGG ACAAGCACCG CTGTGACAAG AAACGGCACC TGGGCAATGA TTTTGTTTCT 
ATCATCTACA ATGATTCTGG TGAGGACTTC AAGCTGGGCA CCATCAAGGG CCAATTCAAC 
TTTGTCCATG TGATCATCAC ACCGCTGGAC TACAAATGCA ACCTGTTGAC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AAATCGTATC TGACCGAAAC 
CTGTCCTTCG TGGCCCGACA GATGGCCCTA CATGCAAATA TGGCCTCTCA GGTACACCAT 
AGCCGCTCCA ACCCGACTGA CATCTATCCC TCCAAGTGGA TTGCCAGGCT TCGCCACATT 
AAGCGTCTCC GCCACCGGAT CCGTGAAGAA GTGCACTATT CAAACCCAAG CTTGCCTCTG 
ATGCACCCTC CAGCCCACAC CAAAGCCCCA GTTCAGGCCT CTGCTGAGCC CACACCCACC 
TACGAGACAG GCCAGCGGAA ACGCCTCATC TCCTCCGTGG ATGATTTCAC AGAGTTTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_013347156.2 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_013202610.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.

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CloneID	OMi104127
Clone ID Related Accession (Same CDS sequence)	XM_013347156.1 , XM_013347156.2
Accession Version	XM_013347156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_013347156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGACTCTGCC GTGGTTCTCG AGGAAGGAAG TCCAGGAGAG 
GCTCATGTAC CAGTGGAGCC CCCCGAGTTG GAGGATTTTG AGGCAGTAGG TACAGACAGG 
CACTGCCAGC GTCCTGACAC CTACAGCAGG TCGTCCTCAG CGTCTAGCCA GGAAGAAAAG 
TCCCAGCTGG AGGAGATGGC TGCCGGAGGC ATCCCCATCG AGCGGGCCAT CTCCGAGGGT 
GCTCGGCCTG CTGTGGACCT CTCCTTCCAA CCCTCACAGC CCCTGAGCAA GTCTAGCTCT 
TCTCCTGAGT TGCAGACCTT GCAAGACATC CTCGGAGACC TAGGGGACAA GGCTGACATT 
GGACGGCTGA GTCCTGAGTC TAAGGTCCGG TCCCAGTCAG GGATCCTGGA TGGGGAAGTG 
GCTACCTGGT CAGCCCCAGG TGAAGAAGGC CGTGTCACAG TCCCACCTGA AGGTCCCCTG 
CCTTCCAGTT CTCCCCGCTC TCCTAGTGGT CTCCGGCCCC GAGGCTATAC CATCTCTGAT 
TCAGCCCCAT CACGAAGGGG CAAGAGGGTG GAGAGGGATA CCTTCAAGAG CAGAGCTGCG 
GCCTCCAGTG CTGAGAAAGT GCCAGGCATT AACCCTAGCT TTGTGTTCCT ACAGCTCTAC 
CACTCACCCT TTTTTGGTGA TGAGTCCAAT AAGCCCATCC TGCTGCCCAA TGAGTCCTTC 
GAGCGGTCAG TGCAGCTCCT TGACCAGATC CCGTCCTATG ACACCCACAA AATTGCTGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTGGCCA TCCTGTCAAA TGAGCACGGC 
TCCTACAGGT ACACGGAGTT TCTGACAGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACTT GGGTGGACTA GATGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCT GTTTTCCACA TTGCCACCCT GATGCCCACC 
AAGGATGTGG ACAAGCACCG CTGTGACAAG AAACGGCACC TGGGCAATGA TTTTGTTTCT 
ATCATCTACA ATGATTCTGG TGAGGACTTC AAGCTGGGCA CCATCAAGGG CCAATTCAAC 
TTTGTCCATG TGATCATCAC ACCGCTGGAC TACAAATGCA ACCTGTTGAC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AAATCGTATC TGACCGAAAC 
CTGTCCTTCG TGGCCCGACA GATGGCCCTA CATGCAAATA TGGCCTCTCA GGTACACCAT 
AGCCGCTCCA ACCCGACTGA CATCTATCCC TCCAAGTGGA TTGCCAGGCT TCGCCACATT 
AAGCGTCTCC GCCACCGGAT CCGTGAAGAA GTGCACTATT CAAACCCAAG CTTGCCTCTG 
ATGCACCCTC CAGCCCACAC CAAAGCCCCA GTTCAGGCCT CTGCTGAGCC CACACCCACC 
TACGAGACAG GCCAGCGGAA ACGCCTCATC TCCTCCGTGG ATGATTTCAC AGAGTTTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013202610.1
CDS	119..5341
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTDSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013347156.2
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013347156.2

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGAAT CCAGACATCC CTCACCAGCG CTAGCCTGGG GTCTGCGGAT 
GAGAACTCTA TGGCCCAGGC TGATGACAGC TTGAAGAATC TCCACTTGGA GCTCACAGAA 
ACGTGTCTGG ACATGATGGC CCGATACGTG TTCTCCAACT TCACTGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCAGGG GGTCGGACCA AGACCTGGCT GGTCGGAAAT 
AAGCTCGTCA CCGTGACAAC CAGCGTGGGA ACCGGCACCC GGTCGTTGCT GGGCCTGGAT 
TCAGGGGATC TGCAGGGTGG CCCAGAGCCA AGCTCTGATC CTGGGGTACA TGTGAGGCAG 
ACAAAGGAAG CACCAGCCAA ACTGGAGTCG CAGTCTGGGC AGCAGGTGTC GCGTGGGGCC 
CGGGACCGGG TCCGCTCCAT GTCAGGGGGA CATGGCCTTC GAGTTGGTGC CCTGGATACT 
CCAGCTCCCC ATACCCCAGG TGGCCCTGCT CCCCTGGGAC CACAGACGGC ACCAGCAGCA 
AAGCCTGAGA AGGCTTCTGC GGGAACCCAG CTTCCGAAAG CAGAGAAGAC GAATTTGGCA 
GCTTATGTAC CTTTGCTGAC CCAGGGCTGG GCTGAAATTT TGGTCCGTAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CGTTCTCCTC AGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAATGCT CTTATGGCTG CCGAGCGATT CAAGGAGCAC 
CGGGACACGG CCCTGTACAA GTCCCTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCACCT 
CCTCTTCCAC GCTCTAACAC AGACTCTGCC GTGGTTCTCG AGGAAGGAAG TCCAGGAGAG 
GCTCATGTAC CAGTGGAGCC CCCCGAGTTG GAGGATTTTG AGGCAGTAGG TACAGACAGG 
CACTGCCAGC GTCCTGACAC CTACAGCAGG TCGTCCTCAG CGTCTAGCCA GGAAGAAAAG 
TCCCAGCTGG AGGAGATGGC TGCCGGAGGC ATCCCCATCG AGCGGGCCAT CTCCGAGGGT 
GCTCGGCCTG CTGTGGACCT CTCCTTCCAA CCCTCACAGC CCCTGAGCAA GTCTAGCTCT 
TCTCCTGAGT TGCAGACCTT GCAAGACATC CTCGGAGACC TAGGGGACAA GGCTGACATT 
GGACGGCTGA GTCCTGAGTC TAAGGTCCGG TCCCAGTCAG GGATCCTGGA TGGGGAAGTG 
GCTACCTGGT CAGCCCCAGG TGAAGAAGGC CGTGTCACAG TCCCACCTGA AGGTCCCCTG 
CCTTCCAGTT CTCCCCGCTC TCCTAGTGGT CTCCGGCCCC GAGGCTATAC CATCTCTGAT 
TCAGCCCCAT CACGAAGGGG CAAGAGGGTG GAGAGGGATA CCTTCAAGAG CAGAGCTGCG 
GCCTCCAGTG CTGAGAAAGT GCCAGGCATT AACCCTAGCT TTGTGTTCCT ACAGCTCTAC 
CACTCACCCT TTTTTGGTGA TGAGTCCAAT AAGCCCATCC TGCTGCCCAA TGAGTCCTTC 
GAGCGGTCAG TGCAGCTCCT TGACCAGATC CCGTCCTATG ACACCCACAA AATTGCTGTC 
CTGTATGTGG GAGAAGGCCA GAGCAACAGT GAGCTGGCCA TCCTGTCAAA TGAGCACGGC 
TCCTACAGGT ACACGGAGTT TCTGACAGGC CTGGGCCGGC TCATCGAGCT CAAGGACTGC 
CAGCCAGACA AGGTGTACTT GGGTGGACTA GATGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCT GTTTTCCACA TTGCCACCCT GATGCCCACC 
AAGGATGTGG ACAAGCACCG CTGTGACAAG AAACGGCACC TGGGCAATGA TTTTGTTTCT 
ATCATCTACA ATGATTCTGG TGAGGACTTC AAGCTGGGCA CCATCAAGGG CCAATTCAAC 
TTTGTCCATG TGATCATCAC ACCGCTGGAC TACAAATGCA ACCTGTTGAC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AAATCGTATC TGACCGAAAC 
CTGTCCTTCG TGGCCCGACA GATGGCCCTA CATGCAAATA TGGCCTCTCA GGTACACCAT 
AGCCGCTCCA ACCCGACTGA CATCTATCCC TCCAAGTGGA TTGCCAGGCT TCGCCACATT 
AAGCGTCTCC GCCACCGGAT CCGTGAAGAA GTGCACTATT CAAACCCAAG CTTGCCTCTG 
ATGCACCCTC CAGCCCACAC CAAAGCCCCA GTTCAGGCCT CTGCTGAGCC CACACCCACC 
TACGAGACAG GCCAGCGGAA ACGCCTCATC TCCTCCGTGG ATGATTTCAC AGAGTTTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsc2 ( XM_026780898.1 ) cDNA ORF clone, Microtus ochrogaster(prairie vole) -> XP_026636699.1 Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.

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CloneID	OMi170855
Clone ID Related Accession (Same CDS sequence)	XM_026780898.1
Accession Version	XM_026780898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Microtus ochrogaster(prairie vole)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_022014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Microtus ochrogaster Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGAATC CAGACATCCC TCACCAGCGC TAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAGCT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CGTGTCTGGA CATGATGGCC CGATACGTGT TCTCCAACTT CACTGCGGTC 
CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC CTGGCAGGGG GTCGGACCAA GACCTGGCTG 
GTCGGAAATA AGCTCGTCAC CGTGACAACC AGCGTGGGAA CCGGCACCCG GTCGTTGCTG 
GGCCTGGATT CAGGGGATCT GCAGGGTGGC CCAGAGCCAA GCTCTGATCC TGGGGTACAT 
GTGAGGCAGA CAAAGGAAGC ACCAGCCAAA CTGGAGTCGC AGTCTGGGCA GCAGGTGTCG 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGAC ATGGCCTTCG AGTTGGTGCC 
CTGGATACTC CAGCTCCCCA TACCCCAGGT GGCCCTGCTC CCCTGGGACC ACAGACGGCA 
CCAGCAGCAA AGCCTGAGAA GGCTTCTGCG GGAACCCAGC TTCCGAAAGC AGAGAAGACG 
AATTTGGCAG CTTATGTACC TTTGCTGACC CAGGGCTGGG CTGAAATTTT GGTCCGTAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC GTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCTC TTATGGCTGC CGAGCGATTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCCCTGTCAG TGCCAGCGGC TGGCACAGCC 
AAGCCACCTC CTCTTCCACG CTCTAACACA GTGGCTTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCTGCCGT GGTTCTCGAG 
GAAGGAAGTC CAGGAGAGGC TCATGTACCA GTGGAGCCCC CCGAGTTGGA GGATTTTGAG 
GCAGTAGGTA CAGACAGGCA CTGCCAGCGT CCTGACACCT ACAGCAGGTC GTCCTCAGCG 
TCTAGCCAGG AAGAAAAGTC CCAGCTGGAG GAGATGGCTG CCGGAGGCAT CCCCATCGAG 
CGGGCCATCT CCGAGGGTGC TCGGCCTGCT GTGGACCTCT CCTTCCAACC CTCACAGCCC 
CTGAGCAAGT CTAGCTCTTC TCCTGAGTTG CAGACCTTGC AAGACATCCT CGGAGACCTA 
GGGGACAAGG CTGACATTGG ACGGCTGAGT CCTGAGTCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGTGGC TACCTGGTCA GCCCCAGGTG AAGAAGGCCG TGTCACAGTC 
CCACCTGAAG GTCCCCTGCC TTCCAGTTCT CCCCGCTCTC CTAGTGGTCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC AGCCCCATCA CGAAGGGGCA AGAGGGTGGA GAGGGATACC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAAGTGC CAGGCATTAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA CTCACCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTG 
CTGCCCAATG AGTCCTTCGA GCGGTCAGTG CAGCTCCTTG ACCAGATCCC GTCCTATGAC 
ACCCACAAAA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAACAGTGA GCTGGCCATC 
CTGTCAAATG AGCACGGCTC CTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGCCGGCTC 
ATCGAGCTCA AGGACTGCCA GCCAGACAAG GTGTACTTGG GTGGACTAGA TGTGTGTGGT 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGATT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA GCTGGGCACC 
ATCAAGGGCC AATTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAATGCAAC 
CTGTTGACCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG CGTGGCCAAA 
ATCGTATCTG ACCGAAACCT GTCCTTCGTG GCCCGACAGA TGGCCCTACA TGCAAATATG 
GCCTCTCAGG TACACCATAG CCGCTCCAAC CCGACTGACA TCTATCCCTC CAAGTGGATT 
GCCAGGCTTC GCCACATTAA GCGTCTCCGC CACCGGATCC GTGAAGAAGT GCACTATTCA 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGT TCAGGCCTCT 
GCTGAGCCCA CACCCACCTA CGAGACAGGC CAGCGGAAAC GCCTCATCTC CTCCGTGGAT 
GATTTCACAG AGTTTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026636699.1
CDS	119..5539
Translation	MAKPASKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECGLNNRIRMIGQICDVAKTKKFEEHAVEALWKSVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMDRFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHVPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSLPLDLEERDVAVYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDCMDLERASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSDPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSAPVKELRESCAAEAFRCRSVSVSEHVVRRIQTSLTSASLGSADENSMAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGPEPSSDPGVHVRQTKEAPAKLESQSGQQVSRGARDRVRSMSGGHGLRVGALDTPAPHTPGGPAPLGPQTAPAAKPEKASAGTQLPKAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVLEEGSPGEAHVPVEPPELEDFEAVGTDRHCQRPDTYSRSSSASSQEEKSQLEEMAAGGIPIERAISEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKADIGRLSPESKVRSQSGILDGEVATWSAPGEEGRVTVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDTFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEVHYSNPSLPLMHPPAHTKAPVQASAEPTPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_026780898.1
Organism	Microtus ochrogaster(prairie vole)
Definition	Microtus ochrogaster TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026780898.1

ORF Insert Sequence:

ATGGCCAAAC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT ACTGTTGGGA 
CTGGGAACAT CAAGGCCAAA TCCCAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACGGCGGAGA TCTTGAGAGA ACTGAGTGGT GAATGTGGCC TCAACAATCG CATCCGTATG 
ATAGGACAGA TATGTGATGT GGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAGGCA 
CTTTGGAAGT CTGTCTCAGA CTTGCTACAG CCAGAGCGAC CACCAGAGGC CCGACATGCA 
GTTCTTGCTT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCTCTTTTCT TCAAAGTGAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTA 
GAAGTTTTTA AGGCCCTCAC AGACAATGGA AGGCACATTA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTC GGCTTATCCT CAGAATTCCT TTTGGTACTC 
GTAAATCTGG TCAAGTTCAA CAGCTGTTAC CTTGACGAAT ACATTGCATC AATGGTTCAC 
ATGATTTGTC TGCTGTGCAT CCAGACAGTG TCTTCTGTGG ACATTGAGGT GTCCTTGCAA 
GTCCTGGATG CTGTGGTCTG CTATAACTGC CTACCAGCTG AGAGTCTGCC CCTGTTCATT 
ATCACCCTCT GCCGCACCAT CAATGTCAAG GAGTTGTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTTC TAGGTACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAAACAGAT CATACATGGA AGATGCCCCA CTGCTGAGAG GAGCCGTGTT CTTCGTGGGG 
ATGGCACTCT GGGGAGCTCA CCGACTCTAT TCTCTCAAGA ACTCCCCCAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCTAT GACCTGTCCT AATGAGGTGG TGTCTTATGA GATTGTTCTG 
TCCATAACAA GACTCATCAA GAAGTATAGA AAGGAGCTCC AGGCTGTGAC ATGGGATATT 
CTGCTGGACA TTATTGAACG ACTGCTTCAG CAACTCCAGA ACCTGGACAG CCCAGAACTC 
AGGACCATTG TCCATGACCT GCTGACCACC GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCTC AGGAAAGATA TTATGAACTG GTGGAGAGCT ATGCAGACCA GAGACCTGAG 
TCTTCTCTCT TAAACTTGAT ATCCTACAGA GCCCAATCCA TCCACCCGGC CAAGGATGGC 
TGGATCCAGA ACTTGCAGTT GTTAATGGAC AGGTTCTTCA GGAACGAGTG CCGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAAC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACGTTCC CGAGGACAAA 
GACCACCAGG TCCGAAAGTT GGCCACCCAG CTGCTGGTGG ACCTGGCAGA AGGCTGCCAC 
ACACACCACT TCAACAGTCT ACTGGACATC ATTGAGAAAG TGATGGCACG CTCGCTCTCT 
CTCCCCCTGG ATCTGGAAGA AAGGGACGTG GCGGTGTACT CAGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTTTATAC CTTGCCTGCC 
AGCCACGCCA CTCGGGTGTA TGAGACCCTC ATTAGCCACA TCCAGCTCCA CTACAAGCAT 
GGCTACTCCC TACCCATTGC CAGCAGCATC CGGCTGCAGG CTTTTGACTT CCTGCTGCTG 
CTGCGGGCTG ATTCGTTGCA TCGACTGGGA CTGCCCAACA AGGATGGGGT CGTGAGATTC 
AGCCCTTACT GTGTCTGTGA CTGCATGGAC CTGGAGAGGG CATCCGAGAA GAAGGCCAGT 
GGGCCTCTTT CACCTCCAAC TGGGCCACCT AGCCCCGTGC CTACAGGCCC TGCTGTACGA 
CTTGGCCACC TACCCTACTC CCTGCTCTTC CGCGTCTTGC TGCAGTGTTT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTACTC AGCAAGCTGC CTGAGTCACT GCGCTACAAA 
GTCCTCATTT TCACCTCCCC CTGCAATGTT GACCAACTGT CTTCTGCCCT CTGCTCCATG 
CTTTCAGACC CAAAGACCCT TGAGCGGCTC AGAGGTACCC CAGAAGGCTT CTCAAGAACC 
GATCTGCACT TGGCTGTGGT CCCTGTGCTG ACGGCATTAA TATCGTACCA CAACTATCTG 
GACAAGACCA AACAGCGTGA GATGGTTTAC TGCTTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GCGTGGTGGC ATTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCTCTGC CTGTCCTGGT AGTGAAGCTG ACGCACATTT CCGCCACAGC CAGTATGGCC 
ATCCCCCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCACCTCTA CAGAAACTTC 
GCTGCAGAGC AGTATGCAAG TGTATTTGCA ATCTCCCTGC CATACACCAA TCCCTCCAAG 
TTCAACCAGT ACATTGTGTG CCTGGCCCAC CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCTT TCCGGAAGGA TTTTGTCCCC TATATTACTA AGGGCCTGCG TTCCAATGTC 
CTCCTGTCTT TCGATGACAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AATGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT 
GCGCCTGTGA AAGAACTCAG AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG AACATGTGGT CCGCAGAATC CAGACATCCC TCACCAGCGC TAGCCTGGGG 
TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAGCT TGAAGAATCT CCACTTGGAG 
CTCACAGAAA CGTGTCTGGA CATGATGGCC CGATACGTGT TCTCCAACTT CACTGCGGTC 
CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC CTGGCAGGGG GTCGGACCAA GACCTGGCTG 
GTCGGAAATA AGCTCGTCAC CGTGACAACC AGCGTGGGAA CCGGCACCCG GTCGTTGCTG 
GGCCTGGATT CAGGGGATCT GCAGGGTGGC CCAGAGCCAA GCTCTGATCC TGGGGTACAT 
GTGAGGCAGA CAAAGGAAGC ACCAGCCAAA CTGGAGTCGC AGTCTGGGCA GCAGGTGTCG 
CGTGGGGCCC GGGACCGGGT CCGCTCCATG TCAGGGGGAC ATGGCCTTCG AGTTGGTGCC 
CTGGATACTC CAGCTCCCCA TACCCCAGGT GGCCCTGCTC CCCTGGGACC ACAGACGGCA 
CCAGCAGCAA AGCCTGAGAA GGCTTCTGCG GGAACCCAGC TTCCGAAAGC AGAGAAGACG 
AATTTGGCAG CTTATGTACC TTTGCTGACC CAGGGCTGGG CTGAAATTTT GGTCCGTAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC GTTCTCCTCA 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAATGCTC TTATGGCTGC CGAGCGATTC 
AAGGAGCACC GGGACACGGC CCTGTACAAG TCCCTGTCAG TGCCAGCGGC TGGCACAGCC 
AAGCCACCTC CTCTTCCACG CTCTAACACA GTGGCTTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCTGCCGT GGTTCTCGAG 
GAAGGAAGTC CAGGAGAGGC TCATGTACCA GTGGAGCCCC CCGAGTTGGA GGATTTTGAG 
GCAGTAGGTA CAGACAGGCA CTGCCAGCGT CCTGACACCT ACAGCAGGTC GTCCTCAGCG 
TCTAGCCAGG AAGAAAAGTC CCAGCTGGAG GAGATGGCTG CCGGAGGCAT CCCCATCGAG 
CGGGCCATCT CCGAGGGTGC TCGGCCTGCT GTGGACCTCT CCTTCCAACC CTCACAGCCC 
CTGAGCAAGT CTAGCTCTTC TCCTGAGTTG CAGACCTTGC AAGACATCCT CGGAGACCTA 
GGGGACAAGG CTGACATTGG ACGGCTGAGT CCTGAGTCTA AGGTCCGGTC CCAGTCAGGG 
ATCCTGGATG GGGAAGTGGC TACCTGGTCA GCCCCAGGTG AAGAAGGCCG TGTCACAGTC 
CCACCTGAAG GTCCCCTGCC TTCCAGTTCT CCCCGCTCTC CTAGTGGTCT CCGGCCCCGA 
GGCTATACCA TCTCTGATTC AGCCCCATCA CGAAGGGGCA AGAGGGTGGA GAGGGATACC 
TTCAAGAGCA GAGCTGCGGC CTCCAGTGCT GAGAAAGTGC CAGGCATTAA CCCTAGCTTT 
GTGTTCCTAC AGCTCTACCA CTCACCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTG 
CTGCCCAATG AGTCCTTCGA GCGGTCAGTG CAGCTCCTTG ACCAGATCCC GTCCTATGAC 
ACCCACAAAA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAACAGTGA GCTGGCCATC 
CTGTCAAATG AGCACGGCTC CTACAGGTAC ACGGAGTTTC TGACAGGCCT GGGCCGGCTC 
ATCGAGCTCA AGGACTGCCA GCCAGACAAG GTGTACTTGG GTGGACTAGA TGTGTGTGGT 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GATGACATCA TGCAAGCTGT TTTCCACATT 
GCCACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGCT GTGACAAGAA ACGGCACCTG 
GGCAATGATT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA GCTGGGCACC 
ATCAAGGGCC AATTCAACTT TGTCCATGTG ATCATCACAC CGCTGGACTA CAAATGCAAC 
CTGTTGACCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG CGTGGCCAAA 
ATCGTATCTG ACCGAAACCT GTCCTTCGTG GCCCGACAGA TGGCCCTACA TGCAAATATG 
GCCTCTCAGG TACACCATAG CCGCTCCAAC CCGACTGACA TCTATCCCTC CAAGTGGATT 
GCCAGGCTTC GCCACATTAA GCGTCTCCGC CACCGGATCC GTGAAGAAGT GCACTATTCA 
AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCACACCA AAGCCCCAGT TCAGGCCTCT 
GCTGAGCCCA CACCCACCTA CGAGACAGGC CAGCGGAAAC GCCTCATCTC CTCCGTGGAT 
GATTTCACAG AGTTTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Tsc2
Entrez Gene ID	101985309
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Microtus ochrogaster(prairie vole)

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Tsc2 cDNA ORF clone, Microtus ochrogaster(prairie vole)

Gene

Clones

gRNAs

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