Evc2 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Evc2
Entrez Gene ID	101836014
Full Name	EvC ciliary complex subunit 2
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013112441.1	XP_012967895.1	limbin
XM_013112441.2	XP_012967895.1	limbin

Pathways from BioSystems
Gene Ontology

The following Evc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Evc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe121991	XM_013112441.2 Latest version!	Mesocricetus auratus EvC ciliary complex subunit 2 (Evc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OMe121991	XM_013112441.1	Mesocricetus auratus Ellis van Creveld syndrome 2 (Evc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Evc2 ( XM_013112441.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012967895.1 Mesocricetus auratus Ellis van Creveld syndrome 2 (Evc2), mRNA.

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CloneID	OMe121991
Clone ID Related Accession (Same CDS sequence)	XM_013112441.1 , XM_013112441.2
Accession Version	XM_013112441.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-12
Organism	Mesocricetus auratus(Golden hamster)
Product	limbin
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801619.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGA CCGGTCCCAC AGGCGCTCGG GGACGGGCCA CGTGGGTGCT GGCTGGGGGC 
GTCCTGGCCG CGGTCCTGGT GCTGGGCGCC GGCCCCCGTA CTTTCTCTCC CTCCTTCCCC 
GCACTGGGCC CGGGGTCTCC ATACAGGCCT GTCCCCGCGG GACCCCAGCC AAGCTCGCAG 
TACAGAGAAG TTTCGAGAGA AGCCCAAGGT CTTTCTGAGA ATGGAGTGAT ATTTCAGAAA 
TGTTCACTGG TGTCTGGGCA GAGCGAATCA CAGGCCATGC TCGTGCAGCT GTCAGTGAAC 
AACACCAGGA CACCCTTGTC AACCAACCTT TCGGACCTGC TGGTGCTGGA CGAGATCACC 
AGCCTCGTTG TGAAGGAGTC CCCTGGAAAT AAGACTCAGA ACGGAATCCA GACTTTCAGA 
AAGAGGTTTC TGCAAGTGGG AGAGTGCTAC TCTGTCAGCT ACATGGCCTC GCTGGACCCC 
ACAGCTCTGG GGACTGGAGA TATCCTGGAC CTTCCAGCCT TGCTCGTCTT CCAGAGCCCC 
TCACAGAACA GAACCCAGCT AAAAGCCCAC TTTACCGTCA CAGTGGAAGA AAAGATAATG 
GTTCTGCCTA ACCATGGCCT CCATGCAGCA GGCTTCATCA CAGCCTTCCT CATCTCCCTC 
GTGCTGACCT TGGTGGCCCT GTTCTTCCTG GCGAAGGGTC GGTGTCTGCA GGGATGTGTG 
CTGGCCAGAT GCCGGGTTCA GCGCCCTGAG AACAAGCTGG AGCCTTCGCC GTTTACCTCT 
GCTAACGGCG TGAGCCAGGA TCTTTCCCTC AATGACCAAG TGATTGACAT CCTGACCTCT 
GAGGACCCTG GAAGCATGCT TCAAGCCTTA GAAGAGTTGG AGATTGCAAC CCTGAATCAG 
GCAGATGCGG ATCTGGAGGC GTGTCGAAAC CAAATTAGTA AGGACATCAT TGCCCTCCTG 
ATGAAGAATC TGACCAGTGG TGGTCACCTC TCTCCACAAA CAGAGAGGAA GGTGACAGCT 
GCTCTCAAAG AGCAGTTTCT GCTGCTGGAA AAGGAAATAC AAGAGGAGTA TGAGCGGAAG 
ATGTTGGCCC TGACAGCAGA GTGTGACCTA GAAGCAAGGA AGAGGACAGA AAGCCAGTAC 
CAGAGGGAGA TGGCCGCCAT GGAGGAAGCA GAAGAGGTGT TGAAGCGAGT TAGTGAGAGG 
TCTGCTGCAG AGTGCAGCAG TCTTCTGCGG ACCCTCCATG GCCTGGAGCA GGAGGACATG 
CAGAGGTCCC TCGCCCTGCA CCAAGCAGAG GACTTTGCCC AAGCACACAG GCAGCTGGTT 
GTTTTCCAGA GAAATGAATT ACGCAGCATC GTCTACACCC AGATACAGAG CGCTGTGGCC 
AAAGGGGAGC TAAGGCCTGA GGTGGCTAAA ATGCTGCTGC AAGACTATTC TAAAACACAG 
GAGCGTGTAG AGGAGCTGAT GGACTTCTTC CAGGCCACCA AGAGATACCA TCTCAGCAAG 
AGGTTTGGTC ATAGGGAGTA CCTGGTCCAG AGCCTGCAGG CCATGGAGAC CCGTGTTCAA 
GGGCTGCTGA GCACTGCGGC TGCTCAGCTG ACAAGCCTCA TCCACAAACA TGAGAGGGCC 
GGGTACTTGG ATGAAGACCA GATGGAAATG CTGTTGGAAC GTGCACAGAC AGAAACCTTT 
TCTATAAAAC AGAAGCTGGA CAATGACTTA AAGCAAGAAA AGAAAAGACT CCACCAAAAA 
TTAATAACTA AGAGAAGGCG GGAACTTCTG CAAAAGCACA AGGAGCAGCA GAAGGAGCAG 
GTGTCCCTTG GCGAGGCCTC CAGCACTGCG GAGGATGCGG TCCAGTACCT GTCCCAGTGG 
AGGAGTGTGA TGGCCGAGCA CACGGCTGCC CTGGAGGAGC TGCAGGAGCG TCTGGACCAG 
GCTGCCCTGG ATGACCTCCG GGTCCTGACC GTGTCTCTGT CTGAGAAAGC CACGGAGGAG 
CTGAGGCGCC TGCAGAGCAC AGCCATGACA CAGGAGCTAC TGAAACGGAG TGCTCCCTGG 
CTCTTCCTGC AGCAGATCTT AGAGGAGCAC AGTCGGGAGT CTGCGGCTCG CACTGCGCAG 
CTGGAGGCAG AGGAGAGAGA GCATGGCCAG GAGCTGGTGC AGGGTGTGAG GCAGCGGCTA 
CAGCAGGATT TACCAGAGGC CTGCACTGAG GAGCAGGCAG AGCTGAGGCA TTGGGAGCAC 
TTGGTGTTCA CGAAGCTCTG CTGTGCGGTC ATCTCACTGT CTGAAGAAGA CCTGCTCAGG 
GTGAGACAGG AGTCCCAGGG CTGCTTCACT CAGCTGGACC GGAGTTTGGC CCTCCCCAGA 
GTCCGGGCGC GAGTTCTGCA GCAGCAGTTA CAGATGGCCA GGCGGGAAGC AGAGTTCCGG 
AAACTGGACC AGGCCCTGGC TGCTCCTGAG CTGCAGTCCA AGGCGAGAAA ACTGCGTTCC 
AAGGGCAGAG GCAAGGCGGA TCTTCTGAAG AAGAACCTGG AGGATAAGAT CCGTCTCTTC 
GAGGAGCGGG CCCCTGGAGA CCTGGCTGAC AAGGTTCGAG GGGAACTGCT CCATGAGCGA 
GTACAGAGGC TGGAGGCCCA GGAGGCCCAC TTTGCTGAGT CACTTGTGGC TCTGCAGTTC 
CAGAAGGTGG CCCGAGCTGC TGAGACCCTC TCTGTCTACA CGGCCTTGCT TACCATTCAA 
GACCTGCTGC TGGGGGAGCT GAGTGAATCC GAGACACTGA CCAAGTCAGC TTGTGTGCAG 
ATCCTGGAGT CCCACAGCCC GGAGCTGCAG GAATTGGAGA GGAAGCTGGA GGAGAGGCTG 
GTGCAGCAGG AAGCAGCCGA GCAGCAGCAG GTCCTGGAGA GCTGGCAGCG GTGGATGGCT 
GAAGGACCAG GGCTGGGCGA ACCTGGGGAG ATGGATCCTG AAAGGCAAGT CTCTGCCACG 
CTGCGGCAGG CCCTGAACAA GAGCCAGAAG CTACTGGAAC AGCAGCAGCA GAGGGTGAGA 
GAGGAGCGGC AGAACAGTGC ATTGCTGGAA GATTCTCTGG AAAGCATGGA GGCTGACACC 
ATGGCCAGTC TGTGCAGCCA GGGACTGAGG CTGGTGTCGT ACCTCTCAAA GATGACAATG 
GTGCCAGGGA GCACTCTACT CCGACTCCTG AGTGTGGTGC TTCCAGTGGC TTCGCAGCCT 
CAGCTACTGG CCCTGCTGGA CACAGTCAGC GAAAAGCACT CAGACCACAC AGCAGAGAAT 
GGGAGCAGTG GGGACCCGGC TGAGCAGGGC AAGAGGAGGA AACACCAGGG TTGGTGGCAA 
GCTTTGGACA GCAGACTGAG AGCAGATCTG GTGAGCCAAG GATTGGAAAG GATGCTTTGG 
GCCCACCAGA AGAAAGAGAG CATATTGAAG AAAGTATACA CTCCTGTCCA GGAGAGGGTG 
ACGTTCCCTG GAAAAGGAAG CTGGCCACAC CTGTCCCTAG AGCCCATTGG GGAACTGGCA 
CACATACCTA TCACAGGGGT GGACACCATG GACATATTAA ACACGGGAGA GAAGATCTTT 
GTATTCAGGA GCCCAAAGGA GCCAGAGATC TCCTTGCGTG TTCCTCCCAG GAGGAAAAAG 
AACTTCTTGA ATGCCAAGAA GGCCAGCAGG GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012967895.1
CDS	39..3686
Translation	MGATGPTGARGRATWVLAGGVLAAVLVLGAGPRTFSPSFPALGPGSPYRPVPAGPQPSSQYREVSREAQGLSENGVIFQKCSLVSGQSESQAMLVQLSVNNTRTPLSTNLSDLLVLDEITSLVVKESPGNKTQNGIQTFRKRFLQVGECYSVSYMASLDPTALGTGDILDLPALLVFQSPSQNRTQLKAHFTVTVEEKIMVLPNHGLHAAGFITAFLISLVLTLVALFFLAKGRCLQGCVLARCRVQRPENKLEPSPFTSANGVSQDLSLNDQVIDILTSEDPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLTSGGHLSPQTERKVTAALKEQFLLLEKEIQEEYERKMLALTAECDLEARKRTESQYQREMAAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDMQRSLALHQAEDFAQAHRQLVVFQRNELRSIVYTQIQSAVAKGELRPEVAKMLLQDYSKTQERVEELMDFFQATKRYHLSKRFGHREYLVQSLQAMETRVQGLLSTAAAQLTSLIHKHERAGYLDEDQMEMLLERAQTETFSIKQKLDNDLKQEKKRLHQKLITKRRRELLQKHKEQQKEQVSLGEASSTAEDAVQYLSQWRSVMAEHTAALEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTAQLEAEEREHGQELVQGVRQRLQQDLPEACTEEQAELRHWEHLVFTKLCCAVISLSEEDLLRVRQESQGCFTQLDRSLALPRVRARVLQQQLQMARREAEFRKLDQALAAPELQSKARKLRSKGRGKADLLKKNLEDKIRLFEERAPGDLADKVRGELLHERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLTIQDLLLGELSESETLTKSACVQILESHSPELQELERKLEERLVQQEAAEQQQVLESWQRWMAEGPGLGEPGEMDPERQVSATLRQALNKSQKLLEQQQQRVREERQNSALLEDSLESMEADTMASLCSQGLRLVSYLSKMTMVPGSTLLRLLSVVLPVASQPQLLALLDTVSEKHSDHTAENGSSGDPAEQGKRRKHQGWWQALDSRLRADLVSQGLERMLWAHQKKESILKKVYTPVQERVTFPGKGSWPHLSLEPIGELAHIPITGVDTMDILNTGEKIFVFRSPKEPEISLRVPPRRKKNFLNAKKASRALGLD

Target ORF information:

RefSeq Version	XM_013112441.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus Ellis van Creveld syndrome 2 (Evc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013112441.1

ORF Insert Sequence:

ATGGGGGCGA CCGGTCCCAC AGGCGCTCGG GGACGGGCCA CGTGGGTGCT GGCTGGGGGC 
GTCCTGGCCG CGGTCCTGGT GCTGGGCGCC GGCCCCCGTA CTTTCTCTCC CTCCTTCCCC 
GCACTGGGCC CGGGGTCTCC ATACAGGCCT GTCCCCGCGG GACCCCAGCC AAGCTCGCAG 
TACAGAGAAG TTTCGAGAGA AGCCCAAGGT CTTTCTGAGA ATGGAGTGAT ATTTCAGAAA 
TGTTCACTGG TGTCTGGGCA GAGCGAATCA CAGGCCATGC TCGTGCAGCT GTCAGTGAAC 
AACACCAGGA CACCCTTGTC AACCAACCTT TCGGACCTGC TGGTGCTGGA CGAGATCACC 
AGCCTCGTTG TGAAGGAGTC CCCTGGAAAT AAGACTCAGA ACGGAATCCA GACTTTCAGA 
AAGAGGTTTC TGCAAGTGGG AGAGTGCTAC TCTGTCAGCT ACATGGCCTC GCTGGACCCC 
ACAGCTCTGG GGACTGGAGA TATCCTGGAC CTTCCAGCCT TGCTCGTCTT CCAGAGCCCC 
TCACAGAACA GAACCCAGCT AAAAGCCCAC TTTACCGTCA CAGTGGAAGA AAAGATAATG 
GTTCTGCCTA ACCATGGCCT CCATGCAGCA GGCTTCATCA CAGCCTTCCT CATCTCCCTC 
GTGCTGACCT TGGTGGCCCT GTTCTTCCTG GCGAAGGGTC GGTGTCTGCA GGGATGTGTG 
CTGGCCAGAT GCCGGGTTCA GCGCCCTGAG AACAAGCTGG AGCCTTCGCC GTTTACCTCT 
GCTAACGGCG TGAGCCAGGA TCTTTCCCTC AATGACCAAG TGATTGACAT CCTGACCTCT 
GAGGACCCTG GAAGCATGCT TCAAGCCTTA GAAGAGTTGG AGATTGCAAC CCTGAATCAG 
GCAGATGCGG ATCTGGAGGC GTGTCGAAAC CAAATTAGTA AGGACATCAT TGCCCTCCTG 
ATGAAGAATC TGACCAGTGG TGGTCACCTC TCTCCACAAA CAGAGAGGAA GGTGACAGCT 
GCTCTCAAAG AGCAGTTTCT GCTGCTGGAA AAGGAAATAC AAGAGGAGTA TGAGCGGAAG 
ATGTTGGCCC TGACAGCAGA GTGTGACCTA GAAGCAAGGA AGAGGACAGA AAGCCAGTAC 
CAGAGGGAGA TGGCCGCCAT GGAGGAAGCA GAAGAGGTGT TGAAGCGAGT TAGTGAGAGG 
TCTGCTGCAG AGTGCAGCAG TCTTCTGCGG ACCCTCCATG GCCTGGAGCA GGAGGACATG 
CAGAGGTCCC TCGCCCTGCA CCAAGCAGAG GACTTTGCCC AAGCACACAG GCAGCTGGTT 
GTTTTCCAGA GAAATGAATT ACGCAGCATC GTCTACACCC AGATACAGAG CGCTGTGGCC 
AAAGGGGAGC TAAGGCCTGA GGTGGCTAAA ATGCTGCTGC AAGACTATTC TAAAACACAG 
GAGCGTGTAG AGGAGCTGAT GGACTTCTTC CAGGCCACCA AGAGATACCA TCTCAGCAAG 
AGGTTTGGTC ATAGGGAGTA CCTGGTCCAG AGCCTGCAGG CCATGGAGAC CCGTGTTCAA 
GGGCTGCTGA GCACTGCGGC TGCTCAGCTG ACAAGCCTCA TCCACAAACA TGAGAGGGCC 
GGGTACTTGG ATGAAGACCA GATGGAAATG CTGTTGGAAC GTGCACAGAC AGAAACCTTT 
TCTATAAAAC AGAAGCTGGA CAATGACTTA AAGCAAGAAA AGAAAAGACT CCACCAAAAA 
TTAATAACTA AGAGAAGGCG GGAACTTCTG CAAAAGCACA AGGAGCAGCA GAAGGAGCAG 
GTGTCCCTTG GCGAGGCCTC CAGCACTGCG GAGGATGCGG TCCAGTACCT GTCCCAGTGG 
AGGAGTGTGA TGGCCGAGCA CACGGCTGCC CTGGAGGAGC TGCAGGAGCG TCTGGACCAG 
GCTGCCCTGG ATGACCTCCG GGTCCTGACC GTGTCTCTGT CTGAGAAAGC CACGGAGGAG 
CTGAGGCGCC TGCAGAGCAC AGCCATGACA CAGGAGCTAC TGAAACGGAG TGCTCCCTGG 
CTCTTCCTGC AGCAGATCTT AGAGGAGCAC AGTCGGGAGT CTGCGGCTCG CACTGCGCAG 
CTGGAGGCAG AGGAGAGAGA GCATGGCCAG GAGCTGGTGC AGGGTGTGAG GCAGCGGCTA 
CAGCAGGATT TACCAGAGGC CTGCACTGAG GAGCAGGCAG AGCTGAGGCA TTGGGAGCAC 
TTGGTGTTCA CGAAGCTCTG CTGTGCGGTC ATCTCACTGT CTGAAGAAGA CCTGCTCAGG 
GTGAGACAGG AGTCCCAGGG CTGCTTCACT CAGCTGGACC GGAGTTTGGC CCTCCCCAGA 
GTCCGGGCGC GAGTTCTGCA GCAGCAGTTA CAGATGGCCA GGCGGGAAGC AGAGTTCCGG 
AAACTGGACC AGGCCCTGGC TGCTCCTGAG CTGCAGTCCA AGGCGAGAAA ACTGCGTTCC 
AAGGGCAGAG GCAAGGCGGA TCTTCTGAAG AAGAACCTGG AGGATAAGAT CCGTCTCTTC 
GAGGAGCGGG CCCCTGGAGA CCTGGCTGAC AAGGTTCGAG GGGAACTGCT CCATGAGCGA 
GTACAGAGGC TGGAGGCCCA GGAGGCCCAC TTTGCTGAGT CACTTGTGGC TCTGCAGTTC 
CAGAAGGTGG CCCGAGCTGC TGAGACCCTC TCTGTCTACA CGGCCTTGCT TACCATTCAA 
GACCTGCTGC TGGGGGAGCT GAGTGAATCC GAGACACTGA CCAAGTCAGC TTGTGTGCAG 
ATCCTGGAGT CCCACAGCCC GGAGCTGCAG GAATTGGAGA GGAAGCTGGA GGAGAGGCTG 
GTGCAGCAGG AAGCAGCCGA GCAGCAGCAG GTCCTGGAGA GCTGGCAGCG GTGGATGGCT 
GAAGGACCAG GGCTGGGCGA ACCTGGGGAG ATGGATCCTG AAAGGCAAGT CTCTGCCACG 
CTGCGGCAGG CCCTGAACAA GAGCCAGAAG CTACTGGAAC AGCAGCAGCA GAGGGTGAGA 
GAGGAGCGGC AGAACAGTGC ATTGCTGGAA GATTCTCTGG AAAGCATGGA GGCTGACACC 
ATGGCCAGTC TGTGCAGCCA GGGACTGAGG CTGGTGTCGT ACCTCTCAAA GATGACAATG 
GTGCCAGGGA GCACTCTACT CCGACTCCTG AGTGTGGTGC TTCCAGTGGC TTCGCAGCCT 
CAGCTACTGG CCCTGCTGGA CACAGTCAGC GAAAAGCACT CAGACCACAC AGCAGAGAAT 
GGGAGCAGTG GGGACCCGGC TGAGCAGGGC AAGAGGAGGA AACACCAGGG TTGGTGGCAA 
GCTTTGGACA GCAGACTGAG AGCAGATCTG GTGAGCCAAG GATTGGAAAG GATGCTTTGG 
GCCCACCAGA AGAAAGAGAG CATATTGAAG AAAGTATACA CTCCTGTCCA GGAGAGGGTG 
ACGTTCCCTG GAAAAGGAAG CTGGCCACAC CTGTCCCTAG AGCCCATTGG GGAACTGGCA 
CACATACCTA TCACAGGGGT GGACACCATG GACATATTAA ACACGGGAGA GAAGATCTTT 
GTATTCAGGA GCCCAAAGGA GCCAGAGATC TCCTTGCGTG TTCCTCCCAG GAGGAAAAAG 
AACTTCTTGA ATGCCAAGAA GGCCAGCAGG GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc2 ( XM_013112441.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012967895.1 Mesocricetus auratus EvC ciliary complex subunit 2 (Evc2), mRNA.

Price & Availability↑

CloneID	OMe121991
Clone ID Related Accession (Same CDS sequence)	XM_013112441.1 , XM_013112441.2
Accession Version	XM_013112441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-21
Organism	Mesocricetus auratus(golden hamster)
Product	limbin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801619.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013112441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGA CCGGTCCCAC AGGCGCTCGG GGACGGGCCA CGTGGGTGCT GGCTGGGGGC 
GTCCTGGCCG CGGTCCTGGT GCTGGGCGCC GGCCCCCGTA CTTTCTCTCC CTCCTTCCCC 
GCACTGGGCC CGGGGTCTCC ATACAGGCCT GTCCCCGCGG GACCCCAGCC AAGCTCGCAG 
TACAGAGAAG TTTCGAGAGA AGCCCAAGGT CTTTCTGAGA ATGGAGTGAT ATTTCAGAAA 
TGTTCACTGG TGTCTGGGCA GAGCGAATCA CAGGCCATGC TCGTGCAGCT GTCAGTGAAC 
AACACCAGGA CACCCTTGTC AACCAACCTT TCGGACCTGC TGGTGCTGGA CGAGATCACC 
AGCCTCGTTG TGAAGGAGTC CCCTGGAAAT AAGACTCAGA ACGGAATCCA GACTTTCAGA 
AAGAGGTTTC TGCAAGTGGG AGAGTGCTAC TCTGTCAGCT ACATGGCCTC GCTGGACCCC 
ACAGCTCTGG GGACTGGAGA TATCCTGGAC CTTCCAGCCT TGCTCGTCTT CCAGAGCCCC 
TCACAGAACA GAACCCAGCT AAAAGCCCAC TTTACCGTCA CAGTGGAAGA AAAGATAATG 
GTTCTGCCTA ACCATGGCCT CCATGCAGCA GGCTTCATCA CAGCCTTCCT CATCTCCCTC 
GTGCTGACCT TGGTGGCCCT GTTCTTCCTG GCGAAGGGTC GGTGTCTGCA GGGATGTGTG 
CTGGCCAGAT GCCGGGTTCA GCGCCCTGAG AACAAGCTGG AGCCTTCGCC GTTTACCTCT 
GCTAACGGCG TGAGCCAGGA TCTTTCCCTC AATGACCAAG TGATTGACAT CCTGACCTCT 
GAGGACCCTG GAAGCATGCT TCAAGCCTTA GAAGAGTTGG AGATTGCAAC CCTGAATCAG 
GCAGATGCGG ATCTGGAGGC GTGTCGAAAC CAAATTAGTA AGGACATCAT TGCCCTCCTG 
ATGAAGAATC TGACCAGTGG TGGTCACCTC TCTCCACAAA CAGAGAGGAA GGTGACAGCT 
GCTCTCAAAG AGCAGTTTCT GCTGCTGGAA AAGGAAATAC AAGAGGAGTA TGAGCGGAAG 
ATGTTGGCCC TGACAGCAGA GTGTGACCTA GAAGCAAGGA AGAGGACAGA AAGCCAGTAC 
CAGAGGGAGA TGGCCGCCAT GGAGGAAGCA GAAGAGGTGT TGAAGCGAGT TAGTGAGAGG 
TCTGCTGCAG AGTGCAGCAG TCTTCTGCGG ACCCTCCATG GCCTGGAGCA GGAGGACATG 
CAGAGGTCCC TCGCCCTGCA CCAAGCAGAG GACTTTGCCC AAGCACACAG GCAGCTGGTT 
GTTTTCCAGA GAAATGAATT ACGCAGCATC GTCTACACCC AGATACAGAG CGCTGTGGCC 
AAAGGGGAGC TAAGGCCTGA GGTGGCTAAA ATGCTGCTGC AAGACTATTC TAAAACACAG 
GAGCGTGTAG AGGAGCTGAT GGACTTCTTC CAGGCCACCA AGAGATACCA TCTCAGCAAG 
AGGTTTGGTC ATAGGGAGTA CCTGGTCCAG AGCCTGCAGG CCATGGAGAC CCGTGTTCAA 
GGGCTGCTGA GCACTGCGGC TGCTCAGCTG ACAAGCCTCA TCCACAAACA TGAGAGGGCC 
GGGTACTTGG ATGAAGACCA GATGGAAATG CTGTTGGAAC GTGCACAGAC AGAAACCTTT 
TCTATAAAAC AGAAGCTGGA CAATGACTTA AAGCAAGAAA AGAAAAGACT CCACCAAAAA 
TTAATAACTA AGAGAAGGCG GGAACTTCTG CAAAAGCACA AGGAGCAGCA GAAGGAGCAG 
GTGTCCCTTG GCGAGGCCTC CAGCACTGCG GAGGATGCGG TCCAGTACCT GTCCCAGTGG 
AGGAGTGTGA TGGCCGAGCA CACGGCTGCC CTGGAGGAGC TGCAGGAGCG TCTGGACCAG 
GCTGCCCTGG ATGACCTCCG GGTCCTGACC GTGTCTCTGT CTGAGAAAGC CACGGAGGAG 
CTGAGGCGCC TGCAGAGCAC AGCCATGACA CAGGAGCTAC TGAAACGGAG TGCTCCCTGG 
CTCTTCCTGC AGCAGATCTT AGAGGAGCAC AGTCGGGAGT CTGCGGCTCG CACTGCGCAG 
CTGGAGGCAG AGGAGAGAGA GCATGGCCAG GAGCTGGTGC AGGGTGTGAG GCAGCGGCTA 
CAGCAGGATT TACCAGAGGC CTGCACTGAG GAGCAGGCAG AGCTGAGGCA TTGGGAGCAC 
TTGGTGTTCA CGAAGCTCTG CTGTGCGGTC ATCTCACTGT CTGAAGAAGA CCTGCTCAGG 
GTGAGACAGG AGTCCCAGGG CTGCTTCACT CAGCTGGACC GGAGTTTGGC CCTCCCCAGA 
GTCCGGGCGC GAGTTCTGCA GCAGCAGTTA CAGATGGCCA GGCGGGAAGC AGAGTTCCGG 
AAACTGGACC AGGCCCTGGC TGCTCCTGAG CTGCAGTCCA AGGCGAGAAA ACTGCGTTCC 
AAGGGCAGAG GCAAGGCGGA TCTTCTGAAG AAGAACCTGG AGGATAAGAT CCGTCTCTTC 
GAGGAGCGGG CCCCTGGAGA CCTGGCTGAC AAGGTTCGAG GGGAACTGCT CCATGAGCGA 
GTACAGAGGC TGGAGGCCCA GGAGGCCCAC TTTGCTGAGT CACTTGTGGC TCTGCAGTTC 
CAGAAGGTGG CCCGAGCTGC TGAGACCCTC TCTGTCTACA CGGCCTTGCT TACCATTCAA 
GACCTGCTGC TGGGGGAGCT GAGTGAATCC GAGACACTGA CCAAGTCAGC TTGTGTGCAG 
ATCCTGGAGT CCCACAGCCC GGAGCTGCAG GAATTGGAGA GGAAGCTGGA GGAGAGGCTG 
GTGCAGCAGG AAGCAGCCGA GCAGCAGCAG GTCCTGGAGA GCTGGCAGCG GTGGATGGCT 
GAAGGACCAG GGCTGGGCGA ACCTGGGGAG ATGGATCCTG AAAGGCAAGT CTCTGCCACG 
CTGCGGCAGG CCCTGAACAA GAGCCAGAAG CTACTGGAAC AGCAGCAGCA GAGGGTGAGA 
GAGGAGCGGC AGAACAGTGC ATTGCTGGAA GATTCTCTGG AAAGCATGGA GGCTGACACC 
ATGGCCAGTC TGTGCAGCCA GGGACTGAGG CTGGTGTCGT ACCTCTCAAA GATGACAATG 
GTGCCAGGGA GCACTCTACT CCGACTCCTG AGTGTGGTGC TTCCAGTGGC TTCGCAGCCT 
CAGCTACTGG CCCTGCTGGA CACAGTCAGC GAAAAGCACT CAGACCACAC AGCAGAGAAT 
GGGAGCAGTG GGGACCCGGC TGAGCAGGGC AAGAGGAGGA AACACCAGGG TTGGTGGCAA 
GCTTTGGACA GCAGACTGAG AGCAGATCTG GTGAGCCAAG GATTGGAAAG GATGCTTTGG 
GCCCACCAGA AGAAAGAGAG CATATTGAAG AAAGTATACA CTCCTGTCCA GGAGAGGGTG 
ACGTTCCCTG GAAAAGGAAG CTGGCCACAC CTGTCCCTAG AGCCCATTGG GGAACTGGCA 
CACATACCTA TCACAGGGGT GGACACCATG GACATATTAA ACACGGGAGA GAAGATCTTT 
GTATTCAGGA GCCCAAAGGA GCCAGAGATC TCCTTGCGTG TTCCTCCCAG GAGGAAAAAG 
AACTTCTTGA ATGCCAAGAA GGCCAGCAGG GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012967895.1
CDS	46..3693
Translation	MGATGPTGARGRATWVLAGGVLAAVLVLGAGPRTFSPSFPALGPGSPYRPVPAGPQPSSQYREVSREAQGLSENGVIFQKCSLVSGQSESQAMLVQLSVNNTRTPLSTNLSDLLVLDEITSLVVKESPGNKTQNGIQTFRKRFLQVGECYSVSYMASLDPTALGTGDILDLPALLVFQSPSQNRTQLKAHFTVTVEEKIMVLPNHGLHAAGFITAFLISLVLTLVALFFLAKGRCLQGCVLARCRVQRPENKLEPSPFTSANGVSQDLSLNDQVIDILTSEDPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLTSGGHLSPQTERKVTAALKEQFLLLEKEIQEEYERKMLALTAECDLEARKRTESQYQREMAAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDMQRSLALHQAEDFAQAHRQLVVFQRNELRSIVYTQIQSAVAKGELRPEVAKMLLQDYSKTQERVEELMDFFQATKRYHLSKRFGHREYLVQSLQAMETRVQGLLSTAAAQLTSLIHKHERAGYLDEDQMEMLLERAQTETFSIKQKLDNDLKQEKKRLHQKLITKRRRELLQKHKEQQKEQVSLGEASSTAEDAVQYLSQWRSVMAEHTAALEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTAQLEAEEREHGQELVQGVRQRLQQDLPEACTEEQAELRHWEHLVFTKLCCAVISLSEEDLLRVRQESQGCFTQLDRSLALPRVRARVLQQQLQMARREAEFRKLDQALAAPELQSKARKLRSKGRGKADLLKKNLEDKIRLFEERAPGDLADKVRGELLHERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLTIQDLLLGELSESETLTKSACVQILESHSPELQELERKLEERLVQQEAAEQQQVLESWQRWMAEGPGLGEPGEMDPERQVSATLRQALNKSQKLLEQQQQRVREERQNSALLEDSLESMEADTMASLCSQGLRLVSYLSKMTMVPGSTLLRLLSVVLPVASQPQLLALLDTVSEKHSDHTAENGSSGDPAEQGKRRKHQGWWQALDSRLRADLVSQGLERMLWAHQKKESILKKVYTPVQERVTFPGKGSWPHLSLEPIGELAHIPITGVDTMDILNTGEKIFVFRSPKEPEISLRVPPRRKKNFLNAKKASRALGLD

Target ORF information:

RefSeq Version	XM_013112441.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus EvC ciliary complex subunit 2 (Evc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013112441.2

ORF Insert Sequence:

ATGGGGGCGA CCGGTCCCAC AGGCGCTCGG GGACGGGCCA CGTGGGTGCT GGCTGGGGGC 
GTCCTGGCCG CGGTCCTGGT GCTGGGCGCC GGCCCCCGTA CTTTCTCTCC CTCCTTCCCC 
GCACTGGGCC CGGGGTCTCC ATACAGGCCT GTCCCCGCGG GACCCCAGCC AAGCTCGCAG 
TACAGAGAAG TTTCGAGAGA AGCCCAAGGT CTTTCTGAGA ATGGAGTGAT ATTTCAGAAA 
TGTTCACTGG TGTCTGGGCA GAGCGAATCA CAGGCCATGC TCGTGCAGCT GTCAGTGAAC 
AACACCAGGA CACCCTTGTC AACCAACCTT TCGGACCTGC TGGTGCTGGA CGAGATCACC 
AGCCTCGTTG TGAAGGAGTC CCCTGGAAAT AAGACTCAGA ACGGAATCCA GACTTTCAGA 
AAGAGGTTTC TGCAAGTGGG AGAGTGCTAC TCTGTCAGCT ACATGGCCTC GCTGGACCCC 
ACAGCTCTGG GGACTGGAGA TATCCTGGAC CTTCCAGCCT TGCTCGTCTT CCAGAGCCCC 
TCACAGAACA GAACCCAGCT AAAAGCCCAC TTTACCGTCA CAGTGGAAGA AAAGATAATG 
GTTCTGCCTA ACCATGGCCT CCATGCAGCA GGCTTCATCA CAGCCTTCCT CATCTCCCTC 
GTGCTGACCT TGGTGGCCCT GTTCTTCCTG GCGAAGGGTC GGTGTCTGCA GGGATGTGTG 
CTGGCCAGAT GCCGGGTTCA GCGCCCTGAG AACAAGCTGG AGCCTTCGCC GTTTACCTCT 
GCTAACGGCG TGAGCCAGGA TCTTTCCCTC AATGACCAAG TGATTGACAT CCTGACCTCT 
GAGGACCCTG GAAGCATGCT TCAAGCCTTA GAAGAGTTGG AGATTGCAAC CCTGAATCAG 
GCAGATGCGG ATCTGGAGGC GTGTCGAAAC CAAATTAGTA AGGACATCAT TGCCCTCCTG 
ATGAAGAATC TGACCAGTGG TGGTCACCTC TCTCCACAAA CAGAGAGGAA GGTGACAGCT 
GCTCTCAAAG AGCAGTTTCT GCTGCTGGAA AAGGAAATAC AAGAGGAGTA TGAGCGGAAG 
ATGTTGGCCC TGACAGCAGA GTGTGACCTA GAAGCAAGGA AGAGGACAGA AAGCCAGTAC 
CAGAGGGAGA TGGCCGCCAT GGAGGAAGCA GAAGAGGTGT TGAAGCGAGT TAGTGAGAGG 
TCTGCTGCAG AGTGCAGCAG TCTTCTGCGG ACCCTCCATG GCCTGGAGCA GGAGGACATG 
CAGAGGTCCC TCGCCCTGCA CCAAGCAGAG GACTTTGCCC AAGCACACAG GCAGCTGGTT 
GTTTTCCAGA GAAATGAATT ACGCAGCATC GTCTACACCC AGATACAGAG CGCTGTGGCC 
AAAGGGGAGC TAAGGCCTGA GGTGGCTAAA ATGCTGCTGC AAGACTATTC TAAAACACAG 
GAGCGTGTAG AGGAGCTGAT GGACTTCTTC CAGGCCACCA AGAGATACCA TCTCAGCAAG 
AGGTTTGGTC ATAGGGAGTA CCTGGTCCAG AGCCTGCAGG CCATGGAGAC CCGTGTTCAA 
GGGCTGCTGA GCACTGCGGC TGCTCAGCTG ACAAGCCTCA TCCACAAACA TGAGAGGGCC 
GGGTACTTGG ATGAAGACCA GATGGAAATG CTGTTGGAAC GTGCACAGAC AGAAACCTTT 
TCTATAAAAC AGAAGCTGGA CAATGACTTA AAGCAAGAAA AGAAAAGACT CCACCAAAAA 
TTAATAACTA AGAGAAGGCG GGAACTTCTG CAAAAGCACA AGGAGCAGCA GAAGGAGCAG 
GTGTCCCTTG GCGAGGCCTC CAGCACTGCG GAGGATGCGG TCCAGTACCT GTCCCAGTGG 
AGGAGTGTGA TGGCCGAGCA CACGGCTGCC CTGGAGGAGC TGCAGGAGCG TCTGGACCAG 
GCTGCCCTGG ATGACCTCCG GGTCCTGACC GTGTCTCTGT CTGAGAAAGC CACGGAGGAG 
CTGAGGCGCC TGCAGAGCAC AGCCATGACA CAGGAGCTAC TGAAACGGAG TGCTCCCTGG 
CTCTTCCTGC AGCAGATCTT AGAGGAGCAC AGTCGGGAGT CTGCGGCTCG CACTGCGCAG 
CTGGAGGCAG AGGAGAGAGA GCATGGCCAG GAGCTGGTGC AGGGTGTGAG GCAGCGGCTA 
CAGCAGGATT TACCAGAGGC CTGCACTGAG GAGCAGGCAG AGCTGAGGCA TTGGGAGCAC 
TTGGTGTTCA CGAAGCTCTG CTGTGCGGTC ATCTCACTGT CTGAAGAAGA CCTGCTCAGG 
GTGAGACAGG AGTCCCAGGG CTGCTTCACT CAGCTGGACC GGAGTTTGGC CCTCCCCAGA 
GTCCGGGCGC GAGTTCTGCA GCAGCAGTTA CAGATGGCCA GGCGGGAAGC AGAGTTCCGG 
AAACTGGACC AGGCCCTGGC TGCTCCTGAG CTGCAGTCCA AGGCGAGAAA ACTGCGTTCC 
AAGGGCAGAG GCAAGGCGGA TCTTCTGAAG AAGAACCTGG AGGATAAGAT CCGTCTCTTC 
GAGGAGCGGG CCCCTGGAGA CCTGGCTGAC AAGGTTCGAG GGGAACTGCT CCATGAGCGA 
GTACAGAGGC TGGAGGCCCA GGAGGCCCAC TTTGCTGAGT CACTTGTGGC TCTGCAGTTC 
CAGAAGGTGG CCCGAGCTGC TGAGACCCTC TCTGTCTACA CGGCCTTGCT TACCATTCAA 
GACCTGCTGC TGGGGGAGCT GAGTGAATCC GAGACACTGA CCAAGTCAGC TTGTGTGCAG 
ATCCTGGAGT CCCACAGCCC GGAGCTGCAG GAATTGGAGA GGAAGCTGGA GGAGAGGCTG 
GTGCAGCAGG AAGCAGCCGA GCAGCAGCAG GTCCTGGAGA GCTGGCAGCG GTGGATGGCT 
GAAGGACCAG GGCTGGGCGA ACCTGGGGAG ATGGATCCTG AAAGGCAAGT CTCTGCCACG 
CTGCGGCAGG CCCTGAACAA GAGCCAGAAG CTACTGGAAC AGCAGCAGCA GAGGGTGAGA 
GAGGAGCGGC AGAACAGTGC ATTGCTGGAA GATTCTCTGG AAAGCATGGA GGCTGACACC 
ATGGCCAGTC TGTGCAGCCA GGGACTGAGG CTGGTGTCGT ACCTCTCAAA GATGACAATG 
GTGCCAGGGA GCACTCTACT CCGACTCCTG AGTGTGGTGC TTCCAGTGGC TTCGCAGCCT 
CAGCTACTGG CCCTGCTGGA CACAGTCAGC GAAAAGCACT CAGACCACAC AGCAGAGAAT 
GGGAGCAGTG GGGACCCGGC TGAGCAGGGC AAGAGGAGGA AACACCAGGG TTGGTGGCAA 
GCTTTGGACA GCAGACTGAG AGCAGATCTG GTGAGCCAAG GATTGGAAAG GATGCTTTGG 
GCCCACCAGA AGAAAGAGAG CATATTGAAG AAAGTATACA CTCCTGTCCA GGAGAGGGTG 
ACGTTCCCTG GAAAAGGAAG CTGGCCACAC CTGTCCCTAG AGCCCATTGG GGAACTGGCA 
CACATACCTA TCACAGGGGT GGACACCATG GACATATTAA ACACGGGAGA GAAGATCTTT 
GTATTCAGGA GCCCAAAGGA GCCAGAGATC TCCTTGCGTG TTCCTCCCAG GAGGAAAAAG 
AACTTCTTGA ATGCCAAGAA GGCCAGCAGG GCCTTGGGCT TGGACTGA