HELB cDNA ORF clone, Mesocricetus auratus(Golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELB
Entrez Gene ID	101833786
Full Name	DNA helicase B
Gene Type	protein-coding
Organism	Mesocricetus auratus(Golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013121560.2	XP_012977014.1	DNA helicase B
XM_013121560.1	XP_012977014.1	DNA helicase B

Pathways from BioSystems
Gene Ontology

The following HELB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe135523	XM_013121560.2 Latest version!	Mesocricetus auratus DNA helicase B (Helb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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OMe135523	XM_013121560.1	Mesocricetus auratus helicase (DNA) B (Helb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELB ( XM_013121560.2 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_012977014.1 Mesocricetus auratus DNA helicase B (Helb), mRNA.

Price & Availability↑

CloneID	OMe135523
Clone ID Related Accession (Same CDS sequence)	XM_013121560.2 , XM_013121560.1
Accession Version	XM_013121560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-21
Organism	Mesocricetus auratus(golden hamster)
Product	DNA helicase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801719.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013121560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAG AGTGTCTTCT TCTCCGCGAG CTGCTGGGCC CTCTGCACCC GGATAAGGAT 
GGCGAAGAGG AGGAGTGTGT ACAGGAGGAG GAGGGGGACC AGGATTTGGA GTTTGTGGAC 
GCCGAGGAGC TCTGCAGTGG CGGCATCAGG GCGGGCAGCC TCCCTGGGCG CATCCGAGTT 
TCTATCCCTG ATGAGAATAC GAAAGAGAGA TGCACCGTGC AAGGACGTTT CCCGCTCATA 
GGTCCTTGGT GGCGAGTGAA GGTTCGGGTT GCAAAGCCTG GGCGATCCAA GGTCTACCAA 
GCTCAAGGAT TTCCTTCCTA CTTCCTACAG TCTGATATGT CACCATCTCA TCGAAAAAAC 
ATCTTTTCAC TGTTTCTTAA AGAATGTAAC TATGCACTCA GAGATGAATT TCTAAAATGG 
GTAGAGAAAG TGTCAGGCTA TGAGAATCTA AACTTTGAAA ATCTGCGGGA GACATTAAGG 
AACTTTGGTG GAAAAACTGA AGAGAAAAGT GAAAAGCAGT CCACACAGAA TGAACGGAAT 
GAGATGAGAT TGCTCGTGGA GGAATCCTTC CCGTTTGTAA GTGTAATGAC AGCTTTGCAG 
TTTCCAAAAG TCATGGAATT CCTTCCAACT CTTCTTCCTC GACACTTTAG AAAGCTTATC 
AGCTCAAGTT CTGAAGAGGT GTTGCAAAAG ATAGAAGAGG CTTTGGGTAC ACATCCATGG 
AAGCTTGGGT TCCGAAGAGT GACCTACCGG GAGATGAAGC TTTTGTCCTG CGAGGCCAGC 
TGGACATCAT TCAGTCAGTG TCCCTCCCTG TTCCAGCTGA TGACTGAGCA GCAGAAGAAT 
GCATTGGTCA TATATTCTAA GCTGAGGCAG ACATGTAGAG AAGATGGGCA CACTTATGTT 
GAAGTAAAAG ACTTGACTTC GGCGGTGTCA GAGCACATGT CTTTCCAAGA AGCGTGTGGG 
TCTCTGGAGT TTATGAAGGG CCTTGACGTG GTGACCTATG AGAAGGACTG CATCTTTCTT 
TCTGAACTGT ACGAGGCTGA GCAAGGCATT GCCTCCTCCA TCTGTGAACT GATGGACAGG 
CGCCCGTGGC ATTTACAAGT GGACGTGAAA AACGTCCTGG CATCAATTTG CAGCGGGGAG 
CCCAAAGATT CAGGAAGTGC TGAGGCAGCG GAAGGAAATG AACCGGATAA CGTGGGGCTA 
GAACAGGGCG AGAATGTTTC AGACCCACAG GGCAGTGGTG ACCATGTTAG CAATAATGGC 
GGTCATGAAA TGGATGCAGA AATAAGTGAT GTCCCACTGG ATCAAGATCA GGTGGCTGCA 
CTGGAAATGA TTTGCGCCAA TGCCGTGACG GTTCTCAGCG GGAAAGGTGG CTGCGGGAAG 
ACCACCATTG TTAGCCATCT CTTTAAGCAC GTGCAGCAGC TGGAAGAAAT TGAAGTGCAA 
CAAGCTTGTG AGGATTTTGA GCAAGACTGG GATGCCTCAG AAGAATGGCT TGCTTTTCCT 
AAGCAGAGTC CAGCAGCCGT GGACAAGGCT ATACAGGTTT TGCTCACGGC GCCTACCGGG 
AAAGCAGCTG CTTTATTGAG GCAGAAAACC CATCTTCTGG CTTACACGCT GTGTCAGGTC 
AATTATAGCT TCTATTTTTG GAGGAAACAT AATGAGGGAA TGCCATGGAA ATTCTCAACA 
GTTCGAGTCC TGGTTGTGGA CGAAGGGAGT TTGGTGTCTG TCGGGATCTT CAAATCAGTA 
TTAAAGATAC TGTGTGAGCA TGCCAAACTC TCTAAGCTTA TAATCCTTGG TGATATTAGA 
CAGTTACCCA GTATCGAACC TGGCAACGTG CTGCAGGATG TTTTTGAGAC TCTGAAGTCA 
AGAGGGTGTG CCATTGAACT AAAGACAAAC CACAGGGCAG AGTCCCAGCT GATCGTGGAC 
AATGCTACGA GAATCTCCAG GCGTCAGTTT CCAGAATTTG ATGTAGAACT AAGTATCACT 
GGTAATCCCA CATTACCCAT CTCCATTCAA GATAAGACAT TTATTTTTAT CAGACTCCCT 
GAAGAGGATA GCAGTTCCCA GTCTTCTAAA GGCGAACACC GATCTAATTT ATATTCTGCA 
GTTACAACTT TGCTTCAAGG AAAGGACTTT GAAAGTGCAA AAACATCACA ATTTATTGCA 
TTTAGAAGGC AAGATTGTGA CCTCATCAAT GACTGCTGCT CTAAGCACTA CACAGGCCAT 
TTAATTAAAG ACCACAAGAA GAGGCTCGTC TTTAGAGTTG GTGATAAAAT CTGTTGTACC 
CGGAATGCAT ACCTCTCTGA TTTACTCCCT GACAAGGATG AGGAAGCTTT CAGGAAAGGC 
TTTGATCTTG TCCTTTCTGA TGCTGCCCCC TCTGATGCTG CCCCCTCTGA TTTCAAAAGC 
AAGCAGAGTT TTGAAAGTGG CATTCGACTG TGTAACGGGG AAATATTTTT CATAACAAAG 
GATGTAACTG ATGTCACTTT CAAAAGAAAA CGACTTTTGA CGATCAATAA CGAGGCTGGC 
TTGGAAGTGA CTGTGGACTT TGGCAAACTC ATGACGAACT GTCAGATAAA GCACGCCTGG 
GCTAGGACTA TTCATACTTT CCAGGGGTCG GAGGAGAGCA CAGTTGCCTA TGTAGTGGGG 
AAGGCAGGCC GCCAGCACTG GCAGCATGTG TACACCGCAG TGACCAGGGG CCGCTCCAGA 
GTGTATGTCA TCGCCAAAGA ATCCGAGCTC AGGAGCGCCA TCCGGAAGCG CAGCTTCCCC 
AGGAAGACGC GTCTGAAGCA CTTCTTGCAG AAGCAGCTCC TCTCCAGCGC CCACGCATCT 
CCCACTGGCA TTCTATCCCA GCCTGCAAGC CCTAGAGCTG GAGAAAGACC CAGCACACAG 
CCTCCAGTCT CACCCTTCTG TAGAACCCCA GACAAGAGAG CTACTCCAAA CAATGCCAGG 
GGTGAAGCAT CTTCAGCCAC CAAGGAGACG TTTGCCCTTT ATGAAGGATG GCTGTCAACT 
TCTTTTAGCG ACATAGATAC AGACGAGGAG TCAGGGCAGC CTAGAGGGTC CAAAAGAACT 
GGTGATGGCT TCCCATTTGA TGAAGAAAGC CCCAGTAAAT TCCGTTTGGT TGAAGAATCA 
TCTCCACAAG TGTCTTCTAG ATTTCAGAAT TTGAGGTTGA GTAACCTAAC CCCTCGACAA 
CTTTTCAAAC CCACTGACAA TCAAGAAACG ATTAAGTCAG GTGTGGTGGG TCACACCGAT 
AATCCCAGCA ATCAGGAAGC TGACACAGGA GGATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012977014.1
CDS	114..3392
Translation	MARECLLLRELLGPLHPDKDGEEEECVQEEEGDQDLEFVDAEELCSGGIRAGSLPGRIRVSIPDENTKERCTVQGRFPLIGPWWRVKVRVAKPGRSKVYQAQGFPSYFLQSDMSPSHRKNIFSLFLKECNYALRDEFLKWVEKVSGYENLNFENLRETLRNFGGKTEEKSEKQSTQNERNEMRLLVEESFPFVSVMTALQFPKVMEFLPTLLPRHFRKLISSSSEEVLQKIEEALGTHPWKLGFRRVTYREMKLLSCEASWTSFSQCPSLFQLMTEQQKNALVIYSKLRQTCREDGHTYVEVKDLTSAVSEHMSFQEACGSLEFMKGLDVVTYEKDCIFLSELYEAEQGIASSICELMDRRPWHLQVDVKNVLASICSGEPKDSGSAEAAEGNEPDNVGLEQGENVSDPQGSGDHVSNNGGHEMDAEISDVPLDQDQVAALEMICANAVTVLSGKGGCGKTTIVSHLFKHVQQLEEIEVQQACEDFEQDWDASEEWLAFPKQSPAAVDKAIQVLLTAPTGKAAALLRQKTHLLAYTLCQVNYSFYFWRKHNEGMPWKFSTVRVLVVDEGSLVSVGIFKSVLKILCEHAKLSKLIILGDIRQLPSIEPGNVLQDVFETLKSRGCAIELKTNHRAESQLIVDNATRISRRQFPEFDVELSITGNPTLPISIQDKTFIFIRLPEEDSSSQSSKGEHRSNLYSAVTTLLQGKDFESAKTSQFIAFRRQDCDLINDCCSKHYTGHLIKDHKKRLVFRVGDKICCTRNAYLSDLLPDKDEEAFRKGFDLVLSDAAPSDAAPSDFKSKQSFESGIRLCNGEIFFITKDVTDVTFKRKRLLTINNEAGLEVTVDFGKLMTNCQIKHAWARTIHTFQGSEESTVAYVVGKAGRQHWQHVYTAVTRGRSRVYVIAKESELRSAIRKRSFPRKTRLKHFLQKQLLSSAHASPTGILSQPASPRAGERPSTQPPVSPFCRTPDKRATPNNARGEASSATKETFALYEGWLSTSFSDIDTDEESGQPRGSKRTGDGFPFDEESPSKFRLVEESSPQVSSRFQNLRLSNLTPRQLFKPTDNQETIKSGVVGHTDNPSNQEADTGGS

Target ORF information:

RefSeq Version	XM_013121560.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus DNA helicase B (Helb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013121560.2

ORF Insert Sequence:

ATGGCTAGAG AGTGTCTTCT TCTCCGCGAG CTGCTGGGCC CTCTGCACCC GGATAAGGAT 
GGCGAAGAGG AGGAGTGTGT ACAGGAGGAG GAGGGGGACC AGGATTTGGA GTTTGTGGAC 
GCCGAGGAGC TCTGCAGTGG CGGCATCAGG GCGGGCAGCC TCCCTGGGCG CATCCGAGTT 
TCTATCCCTG ATGAGAATAC GAAAGAGAGA TGCACCGTGC AAGGACGTTT CCCGCTCATA 
GGTCCTTGGT GGCGAGTGAA GGTTCGGGTT GCAAAGCCTG GGCGATCCAA GGTCTACCAA 
GCTCAAGGAT TTCCTTCCTA CTTCCTACAG TCTGATATGT CACCATCTCA TCGAAAAAAC 
ATCTTTTCAC TGTTTCTTAA AGAATGTAAC TATGCACTCA GAGATGAATT TCTAAAATGG 
GTAGAGAAAG TGTCAGGCTA TGAGAATCTA AACTTTGAAA ATCTGCGGGA GACATTAAGG 
AACTTTGGTG GAAAAACTGA AGAGAAAAGT GAAAAGCAGT CCACACAGAA TGAACGGAAT 
GAGATGAGAT TGCTCGTGGA GGAATCCTTC CCGTTTGTAA GTGTAATGAC AGCTTTGCAG 
TTTCCAAAAG TCATGGAATT CCTTCCAACT CTTCTTCCTC GACACTTTAG AAAGCTTATC 
AGCTCAAGTT CTGAAGAGGT GTTGCAAAAG ATAGAAGAGG CTTTGGGTAC ACATCCATGG 
AAGCTTGGGT TCCGAAGAGT GACCTACCGG GAGATGAAGC TTTTGTCCTG CGAGGCCAGC 
TGGACATCAT TCAGTCAGTG TCCCTCCCTG TTCCAGCTGA TGACTGAGCA GCAGAAGAAT 
GCATTGGTCA TATATTCTAA GCTGAGGCAG ACATGTAGAG AAGATGGGCA CACTTATGTT 
GAAGTAAAAG ACTTGACTTC GGCGGTGTCA GAGCACATGT CTTTCCAAGA AGCGTGTGGG 
TCTCTGGAGT TTATGAAGGG CCTTGACGTG GTGACCTATG AGAAGGACTG CATCTTTCTT 
TCTGAACTGT ACGAGGCTGA GCAAGGCATT GCCTCCTCCA TCTGTGAACT GATGGACAGG 
CGCCCGTGGC ATTTACAAGT GGACGTGAAA AACGTCCTGG CATCAATTTG CAGCGGGGAG 
CCCAAAGATT CAGGAAGTGC TGAGGCAGCG GAAGGAAATG AACCGGATAA CGTGGGGCTA 
GAACAGGGCG AGAATGTTTC AGACCCACAG GGCAGTGGTG ACCATGTTAG CAATAATGGC 
GGTCATGAAA TGGATGCAGA AATAAGTGAT GTCCCACTGG ATCAAGATCA GGTGGCTGCA 
CTGGAAATGA TTTGCGCCAA TGCCGTGACG GTTCTCAGCG GGAAAGGTGG CTGCGGGAAG 
ACCACCATTG TTAGCCATCT CTTTAAGCAC GTGCAGCAGC TGGAAGAAAT TGAAGTGCAA 
CAAGCTTGTG AGGATTTTGA GCAAGACTGG GATGCCTCAG AAGAATGGCT TGCTTTTCCT 
AAGCAGAGTC CAGCAGCCGT GGACAAGGCT ATACAGGTTT TGCTCACGGC GCCTACCGGG 
AAAGCAGCTG CTTTATTGAG GCAGAAAACC CATCTTCTGG CTTACACGCT GTGTCAGGTC 
AATTATAGCT TCTATTTTTG GAGGAAACAT AATGAGGGAA TGCCATGGAA ATTCTCAACA 
GTTCGAGTCC TGGTTGTGGA CGAAGGGAGT TTGGTGTCTG TCGGGATCTT CAAATCAGTA 
TTAAAGATAC TGTGTGAGCA TGCCAAACTC TCTAAGCTTA TAATCCTTGG TGATATTAGA 
CAGTTACCCA GTATCGAACC TGGCAACGTG CTGCAGGATG TTTTTGAGAC TCTGAAGTCA 
AGAGGGTGTG CCATTGAACT AAAGACAAAC CACAGGGCAG AGTCCCAGCT GATCGTGGAC 
AATGCTACGA GAATCTCCAG GCGTCAGTTT CCAGAATTTG ATGTAGAACT AAGTATCACT 
GGTAATCCCA CATTACCCAT CTCCATTCAA GATAAGACAT TTATTTTTAT CAGACTCCCT 
GAAGAGGATA GCAGTTCCCA GTCTTCTAAA GGCGAACACC GATCTAATTT ATATTCTGCA 
GTTACAACTT TGCTTCAAGG AAAGGACTTT GAAAGTGCAA AAACATCACA ATTTATTGCA 
TTTAGAAGGC AAGATTGTGA CCTCATCAAT GACTGCTGCT CTAAGCACTA CACAGGCCAT 
TTAATTAAAG ACCACAAGAA GAGGCTCGTC TTTAGAGTTG GTGATAAAAT CTGTTGTACC 
CGGAATGCAT ACCTCTCTGA TTTACTCCCT GACAAGGATG AGGAAGCTTT CAGGAAAGGC 
TTTGATCTTG TCCTTTCTGA TGCTGCCCCC TCTGATGCTG CCCCCTCTGA TTTCAAAAGC 
AAGCAGAGTT TTGAAAGTGG CATTCGACTG TGTAACGGGG AAATATTTTT CATAACAAAG 
GATGTAACTG ATGTCACTTT CAAAAGAAAA CGACTTTTGA CGATCAATAA CGAGGCTGGC 
TTGGAAGTGA CTGTGGACTT TGGCAAACTC ATGACGAACT GTCAGATAAA GCACGCCTGG 
GCTAGGACTA TTCATACTTT CCAGGGGTCG GAGGAGAGCA CAGTTGCCTA TGTAGTGGGG 
AAGGCAGGCC GCCAGCACTG GCAGCATGTG TACACCGCAG TGACCAGGGG CCGCTCCAGA 
GTGTATGTCA TCGCCAAAGA ATCCGAGCTC AGGAGCGCCA TCCGGAAGCG CAGCTTCCCC 
AGGAAGACGC GTCTGAAGCA CTTCTTGCAG AAGCAGCTCC TCTCCAGCGC CCACGCATCT 
CCCACTGGCA TTCTATCCCA GCCTGCAAGC CCTAGAGCTG GAGAAAGACC CAGCACACAG 
CCTCCAGTCT CACCCTTCTG TAGAACCCCA GACAAGAGAG CTACTCCAAA CAATGCCAGG 
GGTGAAGCAT CTTCAGCCAC CAAGGAGACG TTTGCCCTTT ATGAAGGATG GCTGTCAACT 
TCTTTTAGCG ACATAGATAC AGACGAGGAG TCAGGGCAGC CTAGAGGGTC CAAAAGAACT 
GGTGATGGCT TCCCATTTGA TGAAGAAAGC CCCAGTAAAT TCCGTTTGGT TGAAGAATCA 
TCTCCACAAG TGTCTTCTAG ATTTCAGAAT TTGAGGTTGA GTAACCTAAC CCCTCGACAA 
CTTTTCAAAC CCACTGACAA TCAAGAAACG ATTAAGTCAG GTGTGGTGGG TCACACCGAT 
AATCCCAGCA ATCAGGAAGC TGACACAGGA GGATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( XM_013121560.1 ) cDNA ORF clone, Mesocricetus auratus(Golden hamster) -> XP_012977014.1 Mesocricetus auratus helicase (DNA) B (Helb), mRNA.

Price & Availability↑

CloneID	OMe135523
Clone ID Related Accession (Same CDS sequence)	XM_013121560.2 , XM_013121560.1
Accession Version	XM_013121560.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-12
Organism	Mesocricetus auratus(Golden hamster)
Product	DNA helicase B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801719.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAG AGTGTCTTCT TCTCCGCGAG CTGCTGGGCC CTCTGCACCC GGATAAGGAT 
GGCGAAGAGG AGGAGTGTGT ACAGGAGGAG GAGGGGGACC AGGATTTGGA GTTTGTGGAC 
GCCGAGGAGC TCTGCAGTGG CGGCATCAGG GCGGGCAGCC TCCCTGGGCG CATCCGAGTT 
TCTATCCCTG ATGAGAATAC GAAAGAGAGA TGCACCGTGC AAGGACGTTT CCCGCTCATA 
GGTCCTTGGT GGCGAGTGAA GGTTCGGGTT GCAAAGCCTG GGCGATCCAA GGTCTACCAA 
GCTCAAGGAT TTCCTTCCTA CTTCCTACAG TCTGATATGT CACCATCTCA TCGAAAAAAC 
ATCTTTTCAC TGTTTCTTAA AGAATGTAAC TATGCACTCA GAGATGAATT TCTAAAATGG 
GTAGAGAAAG TGTCAGGCTA TGAGAATCTA AACTTTGAAA ATCTGCGGGA GACATTAAGG 
AACTTTGGTG GAAAAACTGA AGAGAAAAGT GAAAAGCAGT CCACACAGAA TGAACGGAAT 
GAGATGAGAT TGCTCGTGGA GGAATCCTTC CCGTTTGTAA GTGTAATGAC AGCTTTGCAG 
TTTCCAAAAG TCATGGAATT CCTTCCAACT CTTCTTCCTC GACACTTTAG AAAGCTTATC 
AGCTCAAGTT CTGAAGAGGT GTTGCAAAAG ATAGAAGAGG CTTTGGGTAC ACATCCATGG 
AAGCTTGGGT TCCGAAGAGT GACCTACCGG GAGATGAAGC TTTTGTCCTG CGAGGCCAGC 
TGGACATCAT TCAGTCAGTG TCCCTCCCTG TTCCAGCTGA TGACTGAGCA GCAGAAGAAT 
GCATTGGTCA TATATTCTAA GCTGAGGCAG ACATGTAGAG AAGATGGGCA CACTTATGTT 
GAAGTAAAAG ACTTGACTTC GGCGGTGTCA GAGCACATGT CTTTCCAAGA AGCGTGTGGG 
TCTCTGGAGT TTATGAAGGG CCTTGACGTG GTGACCTATG AGAAGGACTG CATCTTTCTT 
TCTGAACTGT ACGAGGCTGA GCAAGGCATT GCCTCCTCCA TCTGTGAACT GATGGACAGG 
CGCCCGTGGC ATTTACAAGT GGACGTGAAA AACGTCCTGG CATCAATTTG CAGCGGGGAG 
CCCAAAGATT CAGGAAGTGC TGAGGCAGCG GAAGGAAATG AACCGGATAA CGTGGGGCTA 
GAACAGGGCG AGAATGTTTC AGACCCACAG GGCAGTGGTG ACCATGTTAG CAATAATGGC 
GGTCATGAAA TGGATGCAGA AATAAGTGAT GTCCCACTGG ATCAAGATCA GGTGGCTGCA 
CTGGAAATGA TTTGCGCCAA TGCCGTGACG GTTCTCAGCG GGAAAGGTGG CTGCGGGAAG 
ACCACCATTG TTAGCCATCT CTTTAAGCAC GTGCAGCAGC TGGAAGAAAT TGAAGTGCAA 
CAAGCTTGTG AGGATTTTGA GCAAGACTGG GATGCCTCAG AAGAATGGCT TGCTTTTCCT 
AAGCAGAGTC CAGCAGCCGT GGACAAGGCT ATACAGGTTT TGCTCACGGC GCCTACCGGG 
AAAGCAGCTG CTTTATTGAG GCAGAAAACC CATCTTCTGG CTTACACGCT GTGTCAGGTC 
AATTATAGCT TCTATTTTTG GAGGAAACAT AATGAGGGAA TGCCATGGAA ATTCTCAACA 
GTTCGAGTCC TGGTTGTGGA CGAAGGGAGT TTGGTGTCTG TCGGGATCTT CAAATCAGTA 
TTAAAGATAC TGTGTGAGCA TGCCAAACTC TCTAAGCTTA TAATCCTTGG TGATATTAGA 
CAGTTACCCA GTATCGAACC TGGCAACGTG CTGCAGGATG TTTTTGAGAC TCTGAAGTCA 
AGAGGGTGTG CCATTGAACT AAAGACAAAC CACAGGGCAG AGTCCCAGCT GATCGTGGAC 
AATGCTACGA GAATCTCCAG GCGTCAGTTT CCAGAATTTG ATGTAGAACT AAGTATCACT 
GGTAATCCCA CATTACCCAT CTCCATTCAA GATAAGACAT TTATTTTTAT CAGACTCCCT 
GAAGAGGATA GCAGTTCCCA GTCTTCTAAA GGCGAACACC GATCTAATTT ATATTCTGCA 
GTTACAACTT TGCTTCAAGG AAAGGACTTT GAAAGTGCAA AAACATCACA ATTTATTGCA 
TTTAGAAGGC AAGATTGTGA CCTCATCAAT GACTGCTGCT CTAAGCACTA CACAGGCCAT 
TTAATTAAAG ACCACAAGAA GAGGCTCGTC TTTAGAGTTG GTGATAAAAT CTGTTGTACC 
CGGAATGCAT ACCTCTCTGA TTTACTCCCT GACAAGGATG AGGAAGCTTT CAGGAAAGGC 
TTTGATCTTG TCCTTTCTGA TGCTGCCCCC TCTGATGCTG CCCCCTCTGA TTTCAAAAGC 
AAGCAGAGTT TTGAAAGTGG CATTCGACTG TGTAACGGGG AAATATTTTT CATAACAAAG 
GATGTAACTG ATGTCACTTT CAAAAGAAAA CGACTTTTGA CGATCAATAA CGAGGCTGGC 
TTGGAAGTGA CTGTGGACTT TGGCAAACTC ATGACGAACT GTCAGATAAA GCACGCCTGG 
GCTAGGACTA TTCATACTTT CCAGGGGTCG GAGGAGAGCA CAGTTGCCTA TGTAGTGGGG 
AAGGCAGGCC GCCAGCACTG GCAGCATGTG TACACCGCAG TGACCAGGGG CCGCTCCAGA 
GTGTATGTCA TCGCCAAAGA ATCCGAGCTC AGGAGCGCCA TCCGGAAGCG CAGCTTCCCC 
AGGAAGACGC GTCTGAAGCA CTTCTTGCAG AAGCAGCTCC TCTCCAGCGC CCACGCATCT 
CCCACTGGCA TTCTATCCCA GCCTGCAAGC CCTAGAGCTG GAGAAAGACC CAGCACACAG 
CCTCCAGTCT CACCCTTCTG TAGAACCCCA GACAAGAGAG CTACTCCAAA CAATGCCAGG 
GGTGAAGCAT CTTCAGCCAC CAAGGAGACG TTTGCCCTTT ATGAAGGATG GCTGTCAACT 
TCTTTTAGCG ACATAGATAC AGACGAGGAG TCAGGGCAGC CTAGAGGGTC CAAAAGAACT 
GGTGATGGCT TCCCATTTGA TGAAGAAAGC CCCAGTAAAT TCCGTTTGGT TGAAGAATCA 
TCTCCACAAG TGTCTTCTAG ATTTCAGAAT TTGAGGTTGA GTAACCTAAC CCCTCGACAA 
CTTTTCAAAC CCACTGACAA TCAAGAAACG ATTAAGTCAG GTGTGGTGGG TCACACCGAT 
AATCCCAGCA ATCAGGAAGC TGACACAGGA GGATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012977014.1
CDS	15..3293
Translation	MARECLLLRELLGPLHPDKDGEEEECVQEEEGDQDLEFVDAEELCSGGIRAGSLPGRIRVSIPDENTKERCTVQGRFPLIGPWWRVKVRVAKPGRSKVYQAQGFPSYFLQSDMSPSHRKNIFSLFLKECNYALRDEFLKWVEKVSGYENLNFENLRETLRNFGGKTEEKSEKQSTQNERNEMRLLVEESFPFVSVMTALQFPKVMEFLPTLLPRHFRKLISSSSEEVLQKIEEALGTHPWKLGFRRVTYREMKLLSCEASWTSFSQCPSLFQLMTEQQKNALVIYSKLRQTCREDGHTYVEVKDLTSAVSEHMSFQEACGSLEFMKGLDVVTYEKDCIFLSELYEAEQGIASSICELMDRRPWHLQVDVKNVLASICSGEPKDSGSAEAAEGNEPDNVGLEQGENVSDPQGSGDHVSNNGGHEMDAEISDVPLDQDQVAALEMICANAVTVLSGKGGCGKTTIVSHLFKHVQQLEEIEVQQACEDFEQDWDASEEWLAFPKQSPAAVDKAIQVLLTAPTGKAAALLRQKTHLLAYTLCQVNYSFYFWRKHNEGMPWKFSTVRVLVVDEGSLVSVGIFKSVLKILCEHAKLSKLIILGDIRQLPSIEPGNVLQDVFETLKSRGCAIELKTNHRAESQLIVDNATRISRRQFPEFDVELSITGNPTLPISIQDKTFIFIRLPEEDSSSQSSKGEHRSNLYSAVTTLLQGKDFESAKTSQFIAFRRQDCDLINDCCSKHYTGHLIKDHKKRLVFRVGDKICCTRNAYLSDLLPDKDEEAFRKGFDLVLSDAAPSDAAPSDFKSKQSFESGIRLCNGEIFFITKDVTDVTFKRKRLLTINNEAGLEVTVDFGKLMTNCQIKHAWARTIHTFQGSEESTVAYVVGKAGRQHWQHVYTAVTRGRSRVYVIAKESELRSAIRKRSFPRKTRLKHFLQKQLLSSAHASPTGILSQPASPRAGERPSTQPPVSPFCRTPDKRATPNNARGEASSATKETFALYEGWLSTSFSDIDTDEESGQPRGSKRTGDGFPFDEESPSKFRLVEESSPQVSSRFQNLRLSNLTPRQLFKPTDNQETIKSGVVGHTDNPSNQEADTGGS

Target ORF information:

RefSeq Version	XM_013121560.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus helicase (DNA) B (Helb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013121560.1

ORF Insert Sequence:

ATGGCTAGAG AGTGTCTTCT TCTCCGCGAG CTGCTGGGCC CTCTGCACCC GGATAAGGAT 
GGCGAAGAGG AGGAGTGTGT ACAGGAGGAG GAGGGGGACC AGGATTTGGA GTTTGTGGAC 
GCCGAGGAGC TCTGCAGTGG CGGCATCAGG GCGGGCAGCC TCCCTGGGCG CATCCGAGTT 
TCTATCCCTG ATGAGAATAC GAAAGAGAGA TGCACCGTGC AAGGACGTTT CCCGCTCATA 
GGTCCTTGGT GGCGAGTGAA GGTTCGGGTT GCAAAGCCTG GGCGATCCAA GGTCTACCAA 
GCTCAAGGAT TTCCTTCCTA CTTCCTACAG TCTGATATGT CACCATCTCA TCGAAAAAAC 
ATCTTTTCAC TGTTTCTTAA AGAATGTAAC TATGCACTCA GAGATGAATT TCTAAAATGG 
GTAGAGAAAG TGTCAGGCTA TGAGAATCTA AACTTTGAAA ATCTGCGGGA GACATTAAGG 
AACTTTGGTG GAAAAACTGA AGAGAAAAGT GAAAAGCAGT CCACACAGAA TGAACGGAAT 
GAGATGAGAT TGCTCGTGGA GGAATCCTTC CCGTTTGTAA GTGTAATGAC AGCTTTGCAG 
TTTCCAAAAG TCATGGAATT CCTTCCAACT CTTCTTCCTC GACACTTTAG AAAGCTTATC 
AGCTCAAGTT CTGAAGAGGT GTTGCAAAAG ATAGAAGAGG CTTTGGGTAC ACATCCATGG 
AAGCTTGGGT TCCGAAGAGT GACCTACCGG GAGATGAAGC TTTTGTCCTG CGAGGCCAGC 
TGGACATCAT TCAGTCAGTG TCCCTCCCTG TTCCAGCTGA TGACTGAGCA GCAGAAGAAT 
GCATTGGTCA TATATTCTAA GCTGAGGCAG ACATGTAGAG AAGATGGGCA CACTTATGTT 
GAAGTAAAAG ACTTGACTTC GGCGGTGTCA GAGCACATGT CTTTCCAAGA AGCGTGTGGG 
TCTCTGGAGT TTATGAAGGG CCTTGACGTG GTGACCTATG AGAAGGACTG CATCTTTCTT 
TCTGAACTGT ACGAGGCTGA GCAAGGCATT GCCTCCTCCA TCTGTGAACT GATGGACAGG 
CGCCCGTGGC ATTTACAAGT GGACGTGAAA AACGTCCTGG CATCAATTTG CAGCGGGGAG 
CCCAAAGATT CAGGAAGTGC TGAGGCAGCG GAAGGAAATG AACCGGATAA CGTGGGGCTA 
GAACAGGGCG AGAATGTTTC AGACCCACAG GGCAGTGGTG ACCATGTTAG CAATAATGGC 
GGTCATGAAA TGGATGCAGA AATAAGTGAT GTCCCACTGG ATCAAGATCA GGTGGCTGCA 
CTGGAAATGA TTTGCGCCAA TGCCGTGACG GTTCTCAGCG GGAAAGGTGG CTGCGGGAAG 
ACCACCATTG TTAGCCATCT CTTTAAGCAC GTGCAGCAGC TGGAAGAAAT TGAAGTGCAA 
CAAGCTTGTG AGGATTTTGA GCAAGACTGG GATGCCTCAG AAGAATGGCT TGCTTTTCCT 
AAGCAGAGTC CAGCAGCCGT GGACAAGGCT ATACAGGTTT TGCTCACGGC GCCTACCGGG 
AAAGCAGCTG CTTTATTGAG GCAGAAAACC CATCTTCTGG CTTACACGCT GTGTCAGGTC 
AATTATAGCT TCTATTTTTG GAGGAAACAT AATGAGGGAA TGCCATGGAA ATTCTCAACA 
GTTCGAGTCC TGGTTGTGGA CGAAGGGAGT TTGGTGTCTG TCGGGATCTT CAAATCAGTA 
TTAAAGATAC TGTGTGAGCA TGCCAAACTC TCTAAGCTTA TAATCCTTGG TGATATTAGA 
CAGTTACCCA GTATCGAACC TGGCAACGTG CTGCAGGATG TTTTTGAGAC TCTGAAGTCA 
AGAGGGTGTG CCATTGAACT AAAGACAAAC CACAGGGCAG AGTCCCAGCT GATCGTGGAC 
AATGCTACGA GAATCTCCAG GCGTCAGTTT CCAGAATTTG ATGTAGAACT AAGTATCACT 
GGTAATCCCA CATTACCCAT CTCCATTCAA GATAAGACAT TTATTTTTAT CAGACTCCCT 
GAAGAGGATA GCAGTTCCCA GTCTTCTAAA GGCGAACACC GATCTAATTT ATATTCTGCA 
GTTACAACTT TGCTTCAAGG AAAGGACTTT GAAAGTGCAA AAACATCACA ATTTATTGCA 
TTTAGAAGGC AAGATTGTGA CCTCATCAAT GACTGCTGCT CTAAGCACTA CACAGGCCAT 
TTAATTAAAG ACCACAAGAA GAGGCTCGTC TTTAGAGTTG GTGATAAAAT CTGTTGTACC 
CGGAATGCAT ACCTCTCTGA TTTACTCCCT GACAAGGATG AGGAAGCTTT CAGGAAAGGC 
TTTGATCTTG TCCTTTCTGA TGCTGCCCCC TCTGATGCTG CCCCCTCTGA TTTCAAAAGC 
AAGCAGAGTT TTGAAAGTGG CATTCGACTG TGTAACGGGG AAATATTTTT CATAACAAAG 
GATGTAACTG ATGTCACTTT CAAAAGAAAA CGACTTTTGA CGATCAATAA CGAGGCTGGC 
TTGGAAGTGA CTGTGGACTT TGGCAAACTC ATGACGAACT GTCAGATAAA GCACGCCTGG 
GCTAGGACTA TTCATACTTT CCAGGGGTCG GAGGAGAGCA CAGTTGCCTA TGTAGTGGGG 
AAGGCAGGCC GCCAGCACTG GCAGCATGTG TACACCGCAG TGACCAGGGG CCGCTCCAGA 
GTGTATGTCA TCGCCAAAGA ATCCGAGCTC AGGAGCGCCA TCCGGAAGCG CAGCTTCCCC 
AGGAAGACGC GTCTGAAGCA CTTCTTGCAG AAGCAGCTCC TCTCCAGCGC CCACGCATCT 
CCCACTGGCA TTCTATCCCA GCCTGCAAGC CCTAGAGCTG GAGAAAGACC CAGCACACAG 
CCTCCAGTCT CACCCTTCTG TAGAACCCCA GACAAGAGAG CTACTCCAAA CAATGCCAGG 
GGTGAAGCAT CTTCAGCCAC CAAGGAGACG TTTGCCCTTT ATGAAGGATG GCTGTCAACT 
TCTTTTAGCG ACATAGATAC AGACGAGGAG TCAGGGCAGC CTAGAGGGTC CAAAAGAACT 
GGTGATGGCT TCCCATTTGA TGAAGAAAGC CCCAGTAAAT TCCGTTTGGT TGAAGAATCA 
TCTCCACAAG TGTCTTCTAG ATTTCAGAAT TTGAGGTTGA GTAACCTAAC CCCTCGACAA 
CTTTTCAAAC CCACTGACAA TCAAGAAACG ATTAAGTCAG GTGTGGTGGG TCACACCGAT 
AATCCCAGCA ATCAGGAAGC TGACACAGGA GGATCTTGA