MANEA cDNA ORF clone, Mesitornis unicolor(brown roatelo)

Gene
Clones
gRNAs

Summary

Gene Symbol	MANEA
Entrez Gene ID	104542240
Full Name	mannosidase endo-alpha
Gene Type	protein-coding
Organism	Mesitornis unicolor(brown roatelo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010186478.1	XP_010184780.1	glycoprotein endo-alpha-1,2-mannosidase

Pathways from BioSystems
Gene Ontology

The following MANEA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MANEA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe81517	XM_010186478.1 Latest version!	Mesitornis unicolor mannosidase, endo-alpha (MANEA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MANEA ( XM_010186478.1 ) cDNA ORF clone, Mesitornis unicolor(brown roatelo) -> XP_010184780.1 Mesitornis unicolor mannosidase, endo-alpha (MANEA), mRNA.

Price & Availability↑

CloneID	OMe81517
Clone ID Related Accession (Same CDS sequence)	XM_010186478.1
Accession Version	XM_010186478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Mesitornis unicolor(brown roatelo)
Product	glycoprotein endo-alpha-1,2-mannosidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010190667.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesitornis unicolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGAT TTCGCAGAAG AACATGCATC ATTTTGTTGC TCTTTATTTT GTTTATTTGC 
TCCATAATGA TGGCTTTGAA GACTCTGAGA CCTGACAGAG CTGGCTTTGG GGATCCATTT 
GGACTTGGTT TGTTGCCTGA GCTTCAGCAA CGGACAGTGC TCCTAGACAA TAAACAGAAT 
TCACTAAACA GGGTTGAAGG AGATAGTGTA ACACGTGTCA GTAATTTGCA TAATATAGCG 
GTCAATACTA TGAAAGCATC TATGCCTCCT GTAAACAAAC TGGAAGAAGA GATGTTTTCT 
CCTAATTATA ACTTCCATAT ATTCTACTAT AGCTGGTTTG GGAATCCACA GTTTGATGGT 
AAATATATTC ACTGGAACCA TCCTTTGTTG CCACATTGGG ATCCCAAAAT TGCGAACAAT 
TATCCAAAGG GAAGGCATAA TCCTCCTGAG GACATTGGGG CCAACTTTTA TCCTGAGCTC 
GGATCTTACA GTTCCAAAGA CCCTTCTGTC ATAGAAGCCC ACATGAAACA AATGCGTTCA 
GCTTCAACTG GTGTAATAGT GCTTTCATGG TATCCACCGG GTATGGCTGA TGAAAATGGA 
GAACCAACTG ATGATTTGGT GCCTGTTATT TTGGATTATG CACATAAATA TAATCTAAAG 
GTCACCTTTC ATATTGAACC TTACAAAGAC AGAGATGATC GCAGTATGTA CCACAATGTC 
AAATACATCA TTGACAAATA TGGAGGCCAC CCAGCCTTTT ACAGGCATAA GACCAGCACA 
GGCAGATTTC TTCCCATGTT TTATGTTTAC GATTCTTACG TAACAATTCC TGAAATATGG 
GCAAATCTGT TAACTCTTTC TGGATCCCAG TCTATTCGAA ATACTCCGTA TGATGCATTA 
TTCATTGCAC TTCTTGTAGA AGAAAGACAT AAGCATGACA TTCAGAGAAG TGGCTTTGAT 
GGAATGTACA CGTACTTTGC CACCAATGGC TTCTCCTATG GCTCTTCTCA TCATAACTGG 
GCAAGCTTAA AAGCCTTTTG TGATACTAAC AACTTAATGT TTATTCCAAG CGTGGGACCA 
GGGTATATTG ATACCAGTAT CCGACCATGG AACAACCACA ACACCCGTAA TCGTGTCAAT 
GGGAAATACT ATGAGACTGC CTTCAGTGCA GCCCTTCTGG TGCGACCAGA AATTATTTCT 
ATTACATCTT TTAATGAATG GCATGAGGGA ACTCAGATTG AAAAAGCTGT CCCCAAACGG 
ACTGGACAGA TGGTTTACCT TGATTATAAG CCCCATAAAC CAAATGTTTA CCTAGAGCTT 
ACTCAGAAGT GGTCTGAGAA GTACAGAAAA GAACAAGAAC AGTGGCTTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010184780.1
CDS	16..1389
Translation	MARFRRRTCIILLLFILFICSIMMALKTLRPDRAGFGDPFGLGLLPELQQRTVLLDNKQNSLNRVEGDSVTRVSNLHNIAVNTMKASMPPVNKLEEEMFSPNYNFHIFYYSWFGNPQFDGKYIHWNHPLLPHWDPKIANNYPKGRHNPPEDIGANFYPELGSYSSKDPSVIEAHMKQMRSASTGVIVLSWYPPGMADENGEPTDDLVPVILDYAHKYNLKVTFHIEPYKDRDDRSMYHNVKYIIDKYGGHPAFYRHKTSTGRFLPMFYVYDSYVTIPEIWANLLTLSGSQSIRNTPYDALFIALLVEERHKHDIQRSGFDGMYTYFATNGFSYGSSHHNWASLKAFCDTNNLMFIPSVGPGYIDTSIRPWNNHNTRNRVNGKYYETAFSAALLVRPEIISITSFNEWHEGTQIEKAVPKRTGQMVYLDYKPHKPNVYLELTQKWSEKYRKEQEQWLM

Target ORF information:

RefSeq Version	XM_010186478.1
Organism	Mesitornis unicolor(brown roatelo)
Definition	Mesitornis unicolor mannosidase, endo-alpha (MANEA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010186478.1

ORF Insert Sequence:

ATGGCACGAT TTCGCAGAAG AACATGCATC ATTTTGTTGC TCTTTATTTT GTTTATTTGC 
TCCATAATGA TGGCTTTGAA GACTCTGAGA CCTGACAGAG CTGGCTTTGG GGATCCATTT 
GGACTTGGTT TGTTGCCTGA GCTTCAGCAA CGGACAGTGC TCCTAGACAA TAAACAGAAT 
TCACTAAACA GGGTTGAAGG AGATAGTGTA ACACGTGTCA GTAATTTGCA TAATATAGCG 
GTCAATACTA TGAAAGCATC TATGCCTCCT GTAAACAAAC TGGAAGAAGA GATGTTTTCT 
CCTAATTATA ACTTCCATAT ATTCTACTAT AGCTGGTTTG GGAATCCACA GTTTGATGGT 
AAATATATTC ACTGGAACCA TCCTTTGTTG CCACATTGGG ATCCCAAAAT TGCGAACAAT 
TATCCAAAGG GAAGGCATAA TCCTCCTGAG GACATTGGGG CCAACTTTTA TCCTGAGCTC 
GGATCTTACA GTTCCAAAGA CCCTTCTGTC ATAGAAGCCC ACATGAAACA AATGCGTTCA 
GCTTCAACTG GTGTAATAGT GCTTTCATGG TATCCACCGG GTATGGCTGA TGAAAATGGA 
GAACCAACTG ATGATTTGGT GCCTGTTATT TTGGATTATG CACATAAATA TAATCTAAAG 
GTCACCTTTC ATATTGAACC TTACAAAGAC AGAGATGATC GCAGTATGTA CCACAATGTC 
AAATACATCA TTGACAAATA TGGAGGCCAC CCAGCCTTTT ACAGGCATAA GACCAGCACA 
GGCAGATTTC TTCCCATGTT TTATGTTTAC GATTCTTACG TAACAATTCC TGAAATATGG 
GCAAATCTGT TAACTCTTTC TGGATCCCAG TCTATTCGAA ATACTCCGTA TGATGCATTA 
TTCATTGCAC TTCTTGTAGA AGAAAGACAT AAGCATGACA TTCAGAGAAG TGGCTTTGAT 
GGAATGTACA CGTACTTTGC CACCAATGGC TTCTCCTATG GCTCTTCTCA TCATAACTGG 
GCAAGCTTAA AAGCCTTTTG TGATACTAAC AACTTAATGT TTATTCCAAG CGTGGGACCA 
GGGTATATTG ATACCAGTAT CCGACCATGG AACAACCACA ACACCCGTAA TCGTGTCAAT 
GGGAAATACT ATGAGACTGC CTTCAGTGCA GCCCTTCTGG TGCGACCAGA AATTATTTCT 
ATTACATCTT TTAATGAATG GCATGAGGGA ACTCAGATTG AAAAAGCTGT CCCCAAACGG 
ACTGGACAGA TGGTTTACCT TGATTATAAG CCCCATAAAC CAAATGTTTA CCTAGAGCTT 
ACTCAGAAGT GGTCTGAGAA GTACAGAAAA GAACAAGAAC AGTGGCTTAT GTGA