PPEF2 cDNA ORF clone, Mesitornis unicolor(brown roatelo)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPEF2
Entrez Gene ID	104542040
Full Name	protein phosphatase with EF-hand domain 2
Gene Type	protein-coding
Organism	Mesitornis unicolor(brown roatelo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010186232.1	XP_010184534.1	serine/threonine-protein phosphatase with EF-hands 2

Pathways from BioSystems
Gene Ontology

The following PPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe81397	XM_010186232.1 Latest version!	Mesitornis unicolor protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPEF2 ( XM_010186232.1 ) cDNA ORF clone, Mesitornis unicolor(brown roatelo) -> XP_010184534.1 Mesitornis unicolor protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mRNA.

Price & Availability↑

CloneID	OMe81397
Clone ID Related Accession (Same CDS sequence)	XM_010186232.1
Accession Version	XM_010186232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Mesitornis unicolor(brown roatelo)
Product	serine/threonine-protein phosphatase with EF-hands 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010190019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesitornis unicolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 12% of CDS bases ##RefSeq-Attributes-END##

ATGGGATCTG GTAGTTCTGT AAATGCCAAC TACAAGTACT CCCTGCAGAA GTCTGAAAAT 
GCTTTCAAGG CGGCTGTTTT GATCCAGCAG TGGTATCGGC GCTACGTTGC TCGGCTGGAA 
ATGCGCCGAC GTTGCACCTG GAGAATCTTT CAATCCATCG AGTACGCTTG CGAGCAGGAT 
CAGATCAAGC TTCACAACTT CTTCAGTTAC CTCATGGACC ACTGCACACC GAGCAGCAGC 
AAAGAGAGGG ATTTTCTCAG TCGCATGTTT ATAAGTGGGG AAAGCTTCAA AGAGGCAGAG 
CTGGAAAAAT ACTGTGACTT TGAATCCATG GAGGTGCCAG ACTCCTACAC TGGACCCCGT 
CTCTCTTTCC CGCTCCTTCC TGACCATGCC ACTGCCTTGC TGGAAGCTTT CAAACAGAAA 
CAAAAGCTCC ACGCTCGCTA TGTCTTAAAC CTTCTGCATG AGACCAGGAA GCACCTCAAG 
CAGTTGCCAA ACATCACTCA CGTCTCCACC TGCTATAGCG AGGAGGTCAC CGTGTGCGGA 
GACCTGCACG GCCAGCTGGA TGACTTGTTC CTCATATTTT ATAAGAATGG CCTTCCTTCC 
CCTTCCAAGT CCTATGTGTT CAACGGGGAC TTTGTAGACA GAGGCAAACA GTCCCTCGAG 
ATCCTCATCG TCCTCTTTAC CTTCCTCTTG ATCTACCCAA AGGAGGTTCA TCTCAACCGT 
GGGAACCACG AGGACCACAT GGTCAACTTA CGCTACGGTT TTGCCAAGGA AGTAATGCAG 
AAATACAAGG TGCATGGGAA GAAAATCTTG AAGATGGTTC AGAACGTCTT CTGCTGGCTG 
CCCCTGGCCA CCTTGATTGA TCAGAAAGTC CTTGTTATAC ACGGAGGCAT CTCTGACACC 
ACTGACCTGG ACATGCTTGA GAAAATACAA AGGCACAAAT TTATTTCTGT ATTAAGAGGG 
AAGAAAAGAA AGGAGTCGAA TAGAAATGTG GAAATACAGG AAATAAACGG GGAGAGCACA 
GTAGAGGCTG GCCCAGCACG TGACGAGGCA GCCCCCAGTT TATCTCTGCA GCCCCAGTCA 
GCCCAGGCTC CCAGCATGGC CAACAGGCTG GAGTTCTCCA GGTGGGTCCG GCAGACGGTG 
CAGGAGCAAA TCGAGCGGTG CCGCCGGATG GTGGACATCA GTGAGTCGGA GGAGGAAGAG 
CTCACCTACT CCAGCATGGT GTCCTTGAAG GATGTGGATG AGCCGTGCTG GACTCGCCAG 
GAGGAGTGGA AGCAGATTTT AGACATTCTC TGGAGTGACC CCATGCCTCA GGAAGGCTGC 
AAAGTAAATA CGGTGCGAGG TGGTGGCTGC TATTTTGGGC CTGATGTGAC AGGGAAGATC 
CTTGAGAAGT ACAACTTGCA GTTCCTCATC CGCTCCCACG AGTGCAAGCA AGAGGGCTAC 
GAGTTCTGCC ACAACCGGAA GGTGCTGACC ATATTTTCAG CCTCAAACTA CTATGAAATC 
GGCAGCAACA GGGGAGCCTA TGTGAAACTG GGACCGGACC TCGTCCCCCA TTTTGTGCAG 
TACCAAGCGA ACAAGACAGC CCATACTCTC ACTATGACCC AAAGGATCAG CAGAGTAGAA 
GAGTCAGCCT TTCGAGCTTT ACGGGAACAG CTCTTTGCTC ACACCTCAGC CCTCATCAAC 
GCGTTCAAGG CCTACGATAA GGACAACACA GGAAGGATCC CGCTCAGCAA CTGGGCGACG 
GCAGTGGAGT CAGTCTTGCA CCTGGGACTG CCCTGGCGAA TGCTGAGGCC GCAGCTGGTG 
CGCAGCACGG CGGATGGCAT GCTGGAGTAC AAGTCCTGGC TCAACGACCT GGCCATGGAG 
CAGCGGAGCC AAGAGCACAT CCAGTCGAGC TTGCTGGAAG TCATTTATCG AAACAGATCC 
AACTTGGAGA CCATATTCAG GATCATAGAC ACAGATCACT CAGGTCTCAT CTCATTTGAG 
GAATTCCACC AAACCTGGAA GCTGTTCAGC TCCCACATGA ACATTGAACT CACGGAGGAC 
GGCATCAACG ACTTGGTTCG CAGCATTGAT TTCAATAAAG ATGGAAACAT CGACTTCAAC 
GAGTTCCTAG AAGCCTTCCG CCTTGTCAAA CAGTCACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010184534.1
CDS	1..2142
Translation	MGSGSSVNANYKYSLQKSENAFKAAVLIQQWYRRYVARLEMRRRCTWRIFQSIEYACEQDQIKLHNFFSYLMDHCTPSSSKERDFLSRMFISGESFKEAELEKYCDFESMEVPDSYTGPRLSFPLLPDHATALLEAFKQKQKLHARYVLNLLHETRKHLKQLPNITHVSTCYSEEVTVCGDLHGQLDDLFLIFYKNGLPSPSKSYVFNGDFVDRGKQSLEILIVLFTFLLIYPKEVHLNRGNHEDHMVNLRYGFAKEVMQKYKVHGKKILKMVQNVFCWLPLATLIDQKVLVIHGGISDTTDLDMLEKIQRHKFISVLRGKKRKESNRNVEIQEINGESTVEAGPARDEAAPSLSLQPQSAQAPSMANRLEFSRWVRQTVQEQIERCRRMVDISESEEEELTYSSMVSLKDVDEPCWTRQEEWKQILDILWSDPMPQEGCKVNTVRGGGCYFGPDVTGKILEKYNLQFLIRSHECKQEGYEFCHNRKVLTIFSASNYYEIGSNRGAYVKLGPDLVPHFVQYQANKTAHTLTMTQRISRVEESAFRALREQLFAHTSALINAFKAYDKDNTGRIPLSNWATAVESVLHLGLPWRMLRPQLVRSTADGMLEYKSWLNDLAMEQRSQEHIQSSLLEVIYRNRSNLETIFRIIDTDHSGLISFEEFHQTWKLFSSHMNIELTEDGINDLVRSIDFNKDGNIDFNEFLEAFRLVKQSQ

Target ORF information:

RefSeq Version	XM_010186232.1
Organism	Mesitornis unicolor(brown roatelo)
Definition	Mesitornis unicolor protein phosphatase, EF-hand calcium binding domain 2 (PPEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010186232.1

ORF Insert Sequence:

ATGGGATCTG GTAGTTCTGT AAATGCCAAC TACAAGTACT CCCTGCAGAA GTCTGAAAAT 
GCTTTCAAGG CGGCTGTTTT GATCCAGCAG TGGTATCGGC GCTACGTTGC TCGGCTGGAA 
ATGCGCCGAC GTTGCACCTG GAGAATCTTT CAATCCATCG AGTACGCTTG CGAGCAGGAT 
CAGATCAAGC TTCACAACTT CTTCAGTTAC CTCATGGACC ACTGCACACC GAGCAGCAGC 
AAAGAGAGGG ATTTTCTCAG TCGCATGTTT ATAAGTGGGG AAAGCTTCAA AGAGGCAGAG 
CTGGAAAAAT ACTGTGACTT TGAATCCATG GAGGTGCCAG ACTCCTACAC TGGACCCCGT 
CTCTCTTTCC CGCTCCTTCC TGACCATGCC ACTGCCTTGC TGGAAGCTTT CAAACAGAAA 
CAAAAGCTCC ACGCTCGCTA TGTCTTAAAC CTTCTGCATG AGACCAGGAA GCACCTCAAG 
CAGTTGCCAA ACATCACTCA CGTCTCCACC TGCTATAGCG AGGAGGTCAC CGTGTGCGGA 
GACCTGCACG GCCAGCTGGA TGACTTGTTC CTCATATTTT ATAAGAATGG CCTTCCTTCC 
CCTTCCAAGT CCTATGTGTT CAACGGGGAC TTTGTAGACA GAGGCAAACA GTCCCTCGAG 
ATCCTCATCG TCCTCTTTAC CTTCCTCTTG ATCTACCCAA AGGAGGTTCA TCTCAACCGT 
GGGAACCACG AGGACCACAT GGTCAACTTA CGCTACGGTT TTGCCAAGGA AGTAATGCAG 
AAATACAAGG TGCATGGGAA GAAAATCTTG AAGATGGTTC AGAACGTCTT CTGCTGGCTG 
CCCCTGGCCA CCTTGATTGA TCAGAAAGTC CTTGTTATAC ACGGAGGCAT CTCTGACACC 
ACTGACCTGG ACATGCTTGA GAAAATACAA AGGCACAAAT TTATTTCTGT ATTAAGAGGG 
AAGAAAAGAA AGGAGTCGAA TAGAAATGTG GAAATACAGG AAATAAACGG GGAGAGCACA 
GTAGAGGCTG GCCCAGCACG TGACGAGGCA GCCCCCAGTT TATCTCTGCA GCCCCAGTCA 
GCCCAGGCTC CCAGCATGGC CAACAGGCTG GAGTTCTCCA GGTGGGTCCG GCAGACGGTG 
CAGGAGCAAA TCGAGCGGTG CCGCCGGATG GTGGACATCA GTGAGTCGGA GGAGGAAGAG 
CTCACCTACT CCAGCATGGT GTCCTTGAAG GATGTGGATG AGCCGTGCTG GACTCGCCAG 
GAGGAGTGGA AGCAGATTTT AGACATTCTC TGGAGTGACC CCATGCCTCA GGAAGGCTGC 
AAAGTAAATA CGGTGCGAGG TGGTGGCTGC TATTTTGGGC CTGATGTGAC AGGGAAGATC 
CTTGAGAAGT ACAACTTGCA GTTCCTCATC CGCTCCCACG AGTGCAAGCA AGAGGGCTAC 
GAGTTCTGCC ACAACCGGAA GGTGCTGACC ATATTTTCAG CCTCAAACTA CTATGAAATC 
GGCAGCAACA GGGGAGCCTA TGTGAAACTG GGACCGGACC TCGTCCCCCA TTTTGTGCAG 
TACCAAGCGA ACAAGACAGC CCATACTCTC ACTATGACCC AAAGGATCAG CAGAGTAGAA 
GAGTCAGCCT TTCGAGCTTT ACGGGAACAG CTCTTTGCTC ACACCTCAGC CCTCATCAAC 
GCGTTCAAGG CCTACGATAA GGACAACACA GGAAGGATCC CGCTCAGCAA CTGGGCGACG 
GCAGTGGAGT CAGTCTTGCA CCTGGGACTG CCCTGGCGAA TGCTGAGGCC GCAGCTGGTG 
CGCAGCACGG CGGATGGCAT GCTGGAGTAC AAGTCCTGGC TCAACGACCT GGCCATGGAG 
CAGCGGAGCC AAGAGCACAT CCAGTCGAGC TTGCTGGAAG TCATTTATCG AAACAGATCC 
AACTTGGAGA CCATATTCAG GATCATAGAC ACAGATCACT CAGGTCTCAT CTCATTTGAG 
GAATTCCACC AAACCTGGAA GCTGTTCAGC TCCCACATGA ACATTGAACT CACGGAGGAC 
GGCATCAACG ACTTGGTTCG CAGCATTGAT TTCAATAAAG ATGGAAACAT CGACTTCAAC 
GAGTTCCTAG AAGCCTTCCG CCTTGTCAAA CAGTCACAGT AG