LOC101881693 cDNA ORF clone, Melopsittacus undulatus(budgerigar)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101881693
Entrez Gene ID	101881693
Full Name	ankyrin repeat and LEM domain containing 2
Synonyms	ANKLE2
Gene Type	protein-coding
Organism	Melopsittacus undulatus(budgerigar)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005147733.2	XP_005147790.1	ankyrin repeat and LEM domain-containing protein 2
XM_005147733.1	XP_005147790.1	ankyrin repeat and LEM domain-containing protein 2

Pathways from BioSystems
Gene Ontology

The following LOC101881693 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101881693 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe150910	XM_005147733.2 Latest version!	Melopsittacus undulatus ankyrin repeat and LEM domain containing 2 (LOC101881693), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
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OMe150910	XM_005147733.1	Melopsittacus undulatus ankyrin repeat and LEM domain containing 2 (LOC101881693), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101881693 ( XM_005147733.2 ) cDNA ORF clone, Melopsittacus undulatus(budgerigar) -> XP_005147790.1 Melopsittacus undulatus ankyrin repeat and LEM domain containing 2 (LOC101881693), mRNA.

Price & Availability↑

CloneID	OMe150910
Clone ID Related Accession (Same CDS sequence)	XM_005147733.2 , XM_005147733.1
Accession Version	XM_005147733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-03
Organism	Melopsittacus undulatus(budgerigar)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004848251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 4, 2019 this sequence version replaced XM_005147733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Melopsittacus undulatus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA TATTAGCCCG ATTGAAGCAG CTCAGTGCTG ATGAGCTTCG ACAAGAAATA 
ATAAGAGCAG GACTGAAATG TGGGCCCATT ACCTCAACCA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCATTGTT GGAGCAGCAA GGAGCTCAGG GGGAAGAGGG ATCTGCTGCG 
TCAGGAGAGG CTGCTGAAAA CGTCCCATCT GACAGCAGCC AGGCAGAAGC ACAGAAGGCT 
TTGAAAACTG CAGCAGTGAA TCACAGTGGT CATGGAAGTG TTCCTGAAGA GGACTTTGGG 
TACTGTGTAG GTCTGAACCC TCCAGAAGAA GATGTGATGC ATGTGAACGG TTCAGCTCCA 
GCCTGTGGAG CAGGATGTGT TGATTCACAA ATTAGTGCTC AGACACCATC CAGAGATCCT 
CCACTGTTCT ATGGTGTTTG TCCTGTTTAT GATGACATAT TGGCAAGAAA TGAGAGAATA 
CATGTTTATG AAGATAGAAA GGAAGCTCTT CAAGCTGTTA AGATGATTAA GGGTTCCCGT 
TTTAAAGCTT TTTCAAACAG AGAGGATGCT GAAAAATTTG CTAAGGGAAT CTGTGATTAT 
TTCCCATCTC CAAGCAAGTC CTCTTCATGT CTGTCTCCAG TGAAAATGGG ATCATTTAAC 
AGAGATGGTT TATGCTCTCC TGAGACTGAA ACTGTTAATA AGGAGAGAGC CAACAGCTAC 
AAAAGTCCCC GTACTCAAGA TCTTACTGCT AAACTTCGGA CAGCTGTTGA GAAAGGAGAT 
ACAGCAACAT TTTCAGACCT TATTTGGAGT AACCCTCGCT ACCTGATTGG GTCAGGAGAC 
AATCCTACAG TTGTACAGGA AGGATGTCGA TACAATGTCA TGCACGTTGC TGCCAAGGAG 
AATCAACCCG GTATCTGCCA GTTATTACTG GACACTTTGG AAAATCCTGA ATTTATGAGG 
CTTATGTATC CAGATGATAA CGATGTCATG CTGAAGAATC GCATCCAGTA TATTGTTGAT 
CTTTACCTTA ATACACCAGA TAAAGTGGGA TTCGATACTC CATTGCATTT TGCCTGTAAG 
TTTGGGAATT TGGACGTTGT TAATGTGCTT ACCTCACACC CAGCTATTGT GAAGAACCCA 
AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AAAGAAGCAA GAATAAATCT 
GCGGAATTGA AAGAAAAATT AAGAGAGTAC TTGAAAGGCC AATACTATGT ACCACTCCTG 
AGAGCAGATG ACAATTCCTC AGCTCCTGTA ATCGGTGCTC CTTGGTCACC TGATCAGATA 
GATGACAGCT CCCAGAGAAC TTTACCTAAA TACATGGGCA GCCCCAAAGA TCCAGTGTTG 
TCCATTAGAG CTTATGCAGG CCCCATGAGT CCCTCAAAGG CTGAAGAATT TCGCAGGCTT 
TGGAAGAATC CGCCTCGAGA AAGAGCTGGC TTCTTTCACA ATGTCAGAAA ATCTGATCTG 
GAGAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AGCTGGGGTT CCCGTGGGTT 
GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GTTGAGAAAA 
TTAGAAGAAT ACCTGAGTCA TCGGGAAATG AGCGAAAAGG CTCAGCAGGA AACAGGGGAA 
AATGAAACCT GCAATAGATA CAAAACTCCA CATCCTTCTG GCAGGAGTAA AAAGTGCTGC 
AATTCTATTT CTGTTGGAGC GTTTCTGGAT GATGATGACG ATGATGACAT GAGCTTAGAA 
GAAATTAAGA ACAGGCAAAA TGCAGCACGG AATATCAGCC AGCCTCTCAT GTCCAAAGAA 
CCCAGCATTG ATGCCATCGG AGATGCTGAG TGTGACATCT TGTCCATGGG GCATACAGTA 
AACATCATAG AAACATCAGT TCACCCCAGG CACTACGAAA ACGCAGCTTC TTCCAGCAAA 
AATGGATTTT GTAGTCCTGT GTCCAGTGAA AGGATCATCA GTGACAGAAA GCATCTGCAT 
GATGGAGAAG AATGCCTTGT AGCGCCTGTT TGCGATTTGA TGTCGGAATT TGAAAGCCTG 
TCATTCCAAG AACAAGACGT TCCCAGAGAA TCAAATAAAA TCACTGCAAA AACTCAGAAT 
GAGAGAATTC TGGCTGAAGG AACAAATCAG ATGAGAAAAT CCAAGGCCTG CAATGCAGTT 
TCACTGGCAT GTTCTTCTGA GTCAAGTGTT ACAGGCAAAC CTGGAGAGAC CAAGTTAAGG 
ACAGAACACA GAATACCACT AGAAAAGGAC AGACAGTCCA GGGAAGCTGG AATTCAGGCT 
AATGAAGCAC AACGTAAGGA ACTTTCTTCC CAGAAATTTC TCTCAAAGAC TTCACCCACA 
AATGACAATT CTAAGCTATT CCTGTTTGGG GAGCAGCCCT CAAAGCTGGA CAGTGATGTC 
TTAGCAGCGA TAGAAGCAGT AGAGGTTGAT CCACAGAAAT ACCCAACAGT CTACAAATGG 
AAACATACAG TGCAGTCGTA CTCTTCATCT GACAGGCAAA GTTGGCCAAG TCCAGCACTG 
AAGGCGAGAT TCAAGTCCCA AACTGTCACT GCTGGCCTTC CAGGTGCTTC TGTTTATCCC 
AATTCAGGAA GAAACAGTCC TGTGACAGGA AGTCCAGGAA AATACAGCAA CGCGGCCTCA 
TTCTCTCCAG ATCCTGGGAG TCCCGGGCGC TACAGTCCAG CTTGTGTCAA CCATGCTCTG 
CTGAAGCTGC GCTACTTTTC AGAGCCTCCT GCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005147790.1
CDS	132..2930
Translation	MDAILARLKQLSADELRQEIIRAGLKCGPITSTTRFIFEKKLAQALLEQQGAQGEEGSAASGEAAENVPSDSSQAEAQKALKTAAVNHSGHGSVPEEDFGYCVGLNPPEEDVMHVNGSAPACGAGCVDSQISAQTPSRDPPLFYGVCPVYDDILARNERIHVYEDRKEALQAVKMIKGSRFKAFSNREDAEKFAKGICDYFPSPSKSSSCLSPVKMGSFNRDGLCSPETETVNKERANSYKSPRTQDLTAKLRTAVEKGDTATFSDLIWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGICQLLLDTLENPEFMRLMYPDDNDVMLKNRIQYIVDLYLNTPDKVGFDTPLHFACKFGNLDVVNVLTSHPAIVKNPRNKYDQTPAEVVCERSKNKSAELKEKLREYLKGQYYVPLLRADDNSSAPVIGAPWSPDQIDDSSQRTLPKYMGSPKDPVLSIRAYAGPMSPSKAEEFRRLWKNPPRERAGFFHNVRKSDLERGVERVGRELAHELGFPWVEYWEFLGCFVDLSSQEGLRKLEEYLSHREMSEKAQQETGENETCNRYKTPHPSGRSKKCCNSISVGAFLDDDDDDDMSLEEIKNRQNAARNISQPLMSKEPSIDAIGDAECDILSMGHTVNIIETSVHPRHYENAASSSKNGFCSPVSSERIISDRKHLHDGEECLVAPVCDLMSEFESLSFQEQDVPRESNKITAKTQNERILAEGTNQMRKSKACNAVSLACSSESSVTGKPGETKLRTEHRIPLEKDRQSREAGIQANEAQRKELSSQKFLSKTSPTNDNSKLFLFGEQPSKLDSDVLAAIEAVEVDPQKYPTVYKWKHTVQSYSSSDRQSWPSPALKARFKSQTVTAGLPGASVYPNSGRNSPVTGSPGKYSNAASFSPDPGSPGRYSPACVNHALLKLRYFSEPPAH

Target ORF information:

RefSeq Version	XM_005147733.2
Organism	Melopsittacus undulatus(budgerigar)
Definition	Melopsittacus undulatus ankyrin repeat and LEM domain containing 2 (LOC101881693), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005147733.2

ORF Insert Sequence:

ATGGATGCAA TATTAGCCCG ATTGAAGCAG CTCAGTGCTG ATGAGCTTCG ACAAGAAATA 
ATAAGAGCAG GACTGAAATG TGGGCCCATT ACCTCAACCA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCATTGTT GGAGCAGCAA GGAGCTCAGG GGGAAGAGGG ATCTGCTGCG 
TCAGGAGAGG CTGCTGAAAA CGTCCCATCT GACAGCAGCC AGGCAGAAGC ACAGAAGGCT 
TTGAAAACTG CAGCAGTGAA TCACAGTGGT CATGGAAGTG TTCCTGAAGA GGACTTTGGG 
TACTGTGTAG GTCTGAACCC TCCAGAAGAA GATGTGATGC ATGTGAACGG TTCAGCTCCA 
GCCTGTGGAG CAGGATGTGT TGATTCACAA ATTAGTGCTC AGACACCATC CAGAGATCCT 
CCACTGTTCT ATGGTGTTTG TCCTGTTTAT GATGACATAT TGGCAAGAAA TGAGAGAATA 
CATGTTTATG AAGATAGAAA GGAAGCTCTT CAAGCTGTTA AGATGATTAA GGGTTCCCGT 
TTTAAAGCTT TTTCAAACAG AGAGGATGCT GAAAAATTTG CTAAGGGAAT CTGTGATTAT 
TTCCCATCTC CAAGCAAGTC CTCTTCATGT CTGTCTCCAG TGAAAATGGG ATCATTTAAC 
AGAGATGGTT TATGCTCTCC TGAGACTGAA ACTGTTAATA AGGAGAGAGC CAACAGCTAC 
AAAAGTCCCC GTACTCAAGA TCTTACTGCT AAACTTCGGA CAGCTGTTGA GAAAGGAGAT 
ACAGCAACAT TTTCAGACCT TATTTGGAGT AACCCTCGCT ACCTGATTGG GTCAGGAGAC 
AATCCTACAG TTGTACAGGA AGGATGTCGA TACAATGTCA TGCACGTTGC TGCCAAGGAG 
AATCAACCCG GTATCTGCCA GTTATTACTG GACACTTTGG AAAATCCTGA ATTTATGAGG 
CTTATGTATC CAGATGATAA CGATGTCATG CTGAAGAATC GCATCCAGTA TATTGTTGAT 
CTTTACCTTA ATACACCAGA TAAAGTGGGA TTCGATACTC CATTGCATTT TGCCTGTAAG 
TTTGGGAATT TGGACGTTGT TAATGTGCTT ACCTCACACC CAGCTATTGT GAAGAACCCA 
AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AAAGAAGCAA GAATAAATCT 
GCGGAATTGA AAGAAAAATT AAGAGAGTAC TTGAAAGGCC AATACTATGT ACCACTCCTG 
AGAGCAGATG ACAATTCCTC AGCTCCTGTA ATCGGTGCTC CTTGGTCACC TGATCAGATA 
GATGACAGCT CCCAGAGAAC TTTACCTAAA TACATGGGCA GCCCCAAAGA TCCAGTGTTG 
TCCATTAGAG CTTATGCAGG CCCCATGAGT CCCTCAAAGG CTGAAGAATT TCGCAGGCTT 
TGGAAGAATC CGCCTCGAGA AAGAGCTGGC TTCTTTCACA ATGTCAGAAA ATCTGATCTG 
GAGAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AGCTGGGGTT CCCGTGGGTT 
GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GTTGAGAAAA 
TTAGAAGAAT ACCTGAGTCA TCGGGAAATG AGCGAAAAGG CTCAGCAGGA AACAGGGGAA 
AATGAAACCT GCAATAGATA CAAAACTCCA CATCCTTCTG GCAGGAGTAA AAAGTGCTGC 
AATTCTATTT CTGTTGGAGC GTTTCTGGAT GATGATGACG ATGATGACAT GAGCTTAGAA 
GAAATTAAGA ACAGGCAAAA TGCAGCACGG AATATCAGCC AGCCTCTCAT GTCCAAAGAA 
CCCAGCATTG ATGCCATCGG AGATGCTGAG TGTGACATCT TGTCCATGGG GCATACAGTA 
AACATCATAG AAACATCAGT TCACCCCAGG CACTACGAAA ACGCAGCTTC TTCCAGCAAA 
AATGGATTTT GTAGTCCTGT GTCCAGTGAA AGGATCATCA GTGACAGAAA GCATCTGCAT 
GATGGAGAAG AATGCCTTGT AGCGCCTGTT TGCGATTTGA TGTCGGAATT TGAAAGCCTG 
TCATTCCAAG AACAAGACGT TCCCAGAGAA TCAAATAAAA TCACTGCAAA AACTCAGAAT 
GAGAGAATTC TGGCTGAAGG AACAAATCAG ATGAGAAAAT CCAAGGCCTG CAATGCAGTT 
TCACTGGCAT GTTCTTCTGA GTCAAGTGTT ACAGGCAAAC CTGGAGAGAC CAAGTTAAGG 
ACAGAACACA GAATACCACT AGAAAAGGAC AGACAGTCCA GGGAAGCTGG AATTCAGGCT 
AATGAAGCAC AACGTAAGGA ACTTTCTTCC CAGAAATTTC TCTCAAAGAC TTCACCCACA 
AATGACAATT CTAAGCTATT CCTGTTTGGG GAGCAGCCCT CAAAGCTGGA CAGTGATGTC 
TTAGCAGCGA TAGAAGCAGT AGAGGTTGAT CCACAGAAAT ACCCAACAGT CTACAAATGG 
AAACATACAG TGCAGTCGTA CTCTTCATCT GACAGGCAAA GTTGGCCAAG TCCAGCACTG 
AAGGCGAGAT TCAAGTCCCA AACTGTCACT GCTGGCCTTC CAGGTGCTTC TGTTTATCCC 
AATTCAGGAA GAAACAGTCC TGTGACAGGA AGTCCAGGAA AATACAGCAA CGCGGCCTCA 
TTCTCTCCAG ATCCTGGGAG TCCCGGGCGC TACAGTCCAG CTTGTGTCAA CCATGCTCTG 
CTGAAGCTGC GCTACTTTTC AGAGCCTCCT GCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101881693 ( XM_005147733.1 ) cDNA ORF clone, Melopsittacus undulatus(budgerigar) -> XP_005147790.1 Melopsittacus undulatus ankyrin repeat and LEM domain containing 2 (LOC101881693), mRNA.

Price & Availability↑

CloneID	OMe150910
Clone ID Related Accession (Same CDS sequence)	XM_005147733.2 , XM_005147733.1
Accession Version	XM_005147733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-13
Organism	Melopsittacus undulatus(budgerigar)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004848251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Melopsittacus undulatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA TATTAGCCCG ATTGAAGCAG CTCAGTGCTG ATGAGCTTCG ACAAGAAATA 
ATAAGAGCAG GACTGAAATG TGGGCCCATT ACCTCAACCA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCATTGTT GGAGCAGCAA GGAGCTCAGG GGGAAGAGGG ATCTGCTGCG 
TCAGGAGAGG CTGCTGAAAA CGTCCCATCT GACAGCAGCC AGGCAGAAGC ACAGAAGGCT 
TTGAAAACTG CAGCAGTGAA TCACAGTGGT CATGGAAGTG TTCCTGAAGA GGACTTTGGG 
TACTGTGTAG GTCTGAACCC TCCAGAAGAA GATGTGATGC ATGTGAACGG TTCAGCTCCA 
GCCTGTGGAG CAGGATGTGT TGATTCACAA ATTAGTGCTC AGACACCATC CAGAGATCCT 
CCACTGTTCT ATGGTGTTTG TCCTGTTTAT GATGACATAT TGGCAAGAAA TGAGAGAATA 
CATGTTTATG AAGATAGAAA GGAAGCTCTT CAAGCTGTTA AGATGATTAA GGGTTCCCGT 
TTTAAAGCTT TTTCAAACAG AGAGGATGCT GAAAAATTTG CTAAGGGAAT CTGTGATTAT 
TTCCCATCTC CAAGCAAGTC CTCTTCATGT CTGTCTCCAG TGAAAATGGG ATCATTTAAC 
AGAGATGGTT TATGCTCTCC TGAGACTGAA ACTGTTAATA AGGAGAGAGC CAACAGCTAC 
AAAAGTCCCC GTACTCAAGA TCTTACTGCT AAACTTCGGA CAGCTGTTGA GAAAGGAGAT 
ACAGCAACAT TTTCAGACCT TATTTGGAGT AACCCTCGCT ACCTGATTGG GTCAGGAGAC 
AATCCTACAG TTGTACAGGA AGGATGTCGA TACAATGTCA TGCACGTTGC TGCCAAGGAG 
AATCAACCCG GTATCTGCCA GTTATTACTG GACACTTTGG AAAATCCTGA ATTTATGAGG 
CTTATGTATC CAGATGATAA CGATGTCATG CTGAAGAATC GCATCCAGTA TATTGTTGAT 
CTTTACCTTA ATACACCAGA TAAAGTGGGA TTCGATACTC CATTGCATTT TGCCTGTAAG 
TTTGGGAATT TGGACGTTGT TAATGTGCTT ACCTCACACC CAGCTATTGT GAAGAACCCA 
AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AAAGAAGCAA GAATAAATCT 
GCGGAATTGA AAGAAAAATT AAGAGAGTAC TTGAAAGGCC AATACTATGT ACCACTCCTG 
AGAGCAGATG ACAATTCCTC AGCTCCTGTA ATCGGTGCTC CTTGGTCACC TGATCAGATA 
GATGACAGCT CCCAGAGAAC TTTACCTAAA TACATGGGCA GCCCCAAAGA TCCAGTGTTG 
TCCATTAGAG CTTATGCAGG CCCCATGAGT CCCTCAAAGG CTGAAGAATT TCGCAGGCTT 
TGGAAGAATC CGCCTCGAGA AAGAGCTGGC TTCTTTCACA ATGTCAGAAA ATCTGATCTG 
GAGAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AGCTGGGGTT CCCGTGGGTT 
GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GTTGAGAAAA 
TTAGAAGAAT ACCTGAGTCA TCGGGAAATG AGCGAAAAGG CTCAGCAGGA AACAGGGGAA 
AATGAAACCT GCAATAGATA CAAAACTCCA CATCCTTCTG GCAGGAGTAA AAAGTGCTGC 
AATTCTATTT CTGTTGGAGC GTTTCTGGAT GATGATGACG ATGATGACAT GAGCTTAGAA 
GAAATTAAGA ACAGGCAAAA TGCAGCACGG AATATCAGCC AGCCTCTCAT GTCCAAAGAA 
CCCAGCATTG ATGCCATCGG AGATGCTGAG TGTGACATCT TGTCCATGGG GCATACAGTA 
AACATCATAG AAACATCAGT TCACCCCAGG CACTACGAAA ACGCAGCTTC TTCCAGCAAA 
AATGGATTTT GTAGTCCTGT GTCCAGTGAA AGGATCATCA GTGACAGAAA GCATCTGCAT 
GATGGAGAAG AATGCCTTGT AGCGCCTGTT TGCGATTTGA TGTCGGAATT TGAAAGCCTG 
TCATTCCAAG AACAAGACGT TCCCAGAGAA TCAAATAAAA TCACTGCAAA AACTCAGAAT 
GAGAGAATTC TGGCTGAAGG AACAAATCAG ATGAGAAAAT CCAAGGCCTG CAATGCAGTT 
TCACTGGCAT GTTCTTCTGA GTCAAGTGTT ACAGGCAAAC CTGGAGAGAC CAAGTTAAGG 
ACAGAACACA GAATACCACT AGAAAAGGAC AGACAGTCCA GGGAAGCTGG AATTCAGGCT 
AATGAAGCAC AACGTAAGGA ACTTTCTTCC CAGAAATTTC TCTCAAAGAC TTCACCCACA 
AATGACAATT CTAAGCTATT CCTGTTTGGG GAGCAGCCCT CAAAGCTGGA CAGTGATGTC 
TTAGCAGCGA TAGAAGCAGT AGAGGTTGAT CCACAGAAAT ACCCAACAGT CTACAAATGG 
AAACATACAG TGCAGTCGTA CTCTTCATCT GACAGGCAAA GTTGGCCAAG TCCAGCACTG 
AAGGCGAGAT TCAAGTCCCA AACTGTCACT GCTGGCCTTC CAGGTGCTTC TGTTTATCCC 
AATTCAGGAA GAAACAGTCC TGTGACAGGA AGTCCAGGAA AATACAGCAA CGCGGCCTCA 
TTCTCTCCAG ATCCTGGGAG TCCCGGGCGC TACAGTCCAG CTTGTGTCAA CCATGCTCTG 
CTGAAGCTGC GCTACTTTTC AGAGCCTCCT GCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005147790.1
CDS	4..2802
Translation	MDAILARLKQLSADELRQEIIRAGLKCGPITSTTRFIFEKKLAQALLEQQGAQGEEGSAASGEAAENVPSDSSQAEAQKALKTAAVNHSGHGSVPEEDFGYCVGLNPPEEDVMHVNGSAPACGAGCVDSQISAQTPSRDPPLFYGVCPVYDDILARNERIHVYEDRKEALQAVKMIKGSRFKAFSNREDAEKFAKGICDYFPSPSKSSSCLSPVKMGSFNRDGLCSPETETVNKERANSYKSPRTQDLTAKLRTAVEKGDTATFSDLIWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGICQLLLDTLENPEFMRLMYPDDNDVMLKNRIQYIVDLYLNTPDKVGFDTPLHFACKFGNLDVVNVLTSHPAIVKNPRNKYDQTPAEVVCERSKNKSAELKEKLREYLKGQYYVPLLRADDNSSAPVIGAPWSPDQIDDSSQRTLPKYMGSPKDPVLSIRAYAGPMSPSKAEEFRRLWKNPPRERAGFFHNVRKSDLERGVERVGRELAHELGFPWVEYWEFLGCFVDLSSQEGLRKLEEYLSHREMSEKAQQETGENETCNRYKTPHPSGRSKKCCNSISVGAFLDDDDDDDMSLEEIKNRQNAARNISQPLMSKEPSIDAIGDAECDILSMGHTVNIIETSVHPRHYENAASSSKNGFCSPVSSERIISDRKHLHDGEECLVAPVCDLMSEFESLSFQEQDVPRESNKITAKTQNERILAEGTNQMRKSKACNAVSLACSSESSVTGKPGETKLRTEHRIPLEKDRQSREAGIQANEAQRKELSSQKFLSKTSPTNDNSKLFLFGEQPSKLDSDVLAAIEAVEVDPQKYPTVYKWKHTVQSYSSSDRQSWPSPALKARFKSQTVTAGLPGASVYPNSGRNSPVTGSPGKYSNAASFSPDPGSPGRYSPACVNHALLKLRYFSEPPAH

Target ORF information:

RefSeq Version	XM_005147733.1
Organism	Melopsittacus undulatus(budgerigar)
Definition	Melopsittacus undulatus ankyrin repeat and LEM domain containing 2 (LOC101881693), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005147733.1

ORF Insert Sequence:

ATGGATGCAA TATTAGCCCG ATTGAAGCAG CTCAGTGCTG ATGAGCTTCG ACAAGAAATA 
ATAAGAGCAG GACTGAAATG TGGGCCCATT ACCTCAACCA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCATTGTT GGAGCAGCAA GGAGCTCAGG GGGAAGAGGG ATCTGCTGCG 
TCAGGAGAGG CTGCTGAAAA CGTCCCATCT GACAGCAGCC AGGCAGAAGC ACAGAAGGCT 
TTGAAAACTG CAGCAGTGAA TCACAGTGGT CATGGAAGTG TTCCTGAAGA GGACTTTGGG 
TACTGTGTAG GTCTGAACCC TCCAGAAGAA GATGTGATGC ATGTGAACGG TTCAGCTCCA 
GCCTGTGGAG CAGGATGTGT TGATTCACAA ATTAGTGCTC AGACACCATC CAGAGATCCT 
CCACTGTTCT ATGGTGTTTG TCCTGTTTAT GATGACATAT TGGCAAGAAA TGAGAGAATA 
CATGTTTATG AAGATAGAAA GGAAGCTCTT CAAGCTGTTA AGATGATTAA GGGTTCCCGT 
TTTAAAGCTT TTTCAAACAG AGAGGATGCT GAAAAATTTG CTAAGGGAAT CTGTGATTAT 
TTCCCATCTC CAAGCAAGTC CTCTTCATGT CTGTCTCCAG TGAAAATGGG ATCATTTAAC 
AGAGATGGTT TATGCTCTCC TGAGACTGAA ACTGTTAATA AGGAGAGAGC CAACAGCTAC 
AAAAGTCCCC GTACTCAAGA TCTTACTGCT AAACTTCGGA CAGCTGTTGA GAAAGGAGAT 
ACAGCAACAT TTTCAGACCT TATTTGGAGT AACCCTCGCT ACCTGATTGG GTCAGGAGAC 
AATCCTACAG TTGTACAGGA AGGATGTCGA TACAATGTCA TGCACGTTGC TGCCAAGGAG 
AATCAACCCG GTATCTGCCA GTTATTACTG GACACTTTGG AAAATCCTGA ATTTATGAGG 
CTTATGTATC CAGATGATAA CGATGTCATG CTGAAGAATC GCATCCAGTA TATTGTTGAT 
CTTTACCTTA ATACACCAGA TAAAGTGGGA TTCGATACTC CATTGCATTT TGCCTGTAAG 
TTTGGGAATT TGGACGTTGT TAATGTGCTT ACCTCACACC CAGCTATTGT GAAGAACCCA 
AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AAAGAAGCAA GAATAAATCT 
GCGGAATTGA AAGAAAAATT AAGAGAGTAC TTGAAAGGCC AATACTATGT ACCACTCCTG 
AGAGCAGATG ACAATTCCTC AGCTCCTGTA ATCGGTGCTC CTTGGTCACC TGATCAGATA 
GATGACAGCT CCCAGAGAAC TTTACCTAAA TACATGGGCA GCCCCAAAGA TCCAGTGTTG 
TCCATTAGAG CTTATGCAGG CCCCATGAGT CCCTCAAAGG CTGAAGAATT TCGCAGGCTT 
TGGAAGAATC CGCCTCGAGA AAGAGCTGGC TTCTTTCACA ATGTCAGAAA ATCTGATCTG 
GAGAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AGCTGGGGTT CCCGTGGGTT 
GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GTTGAGAAAA 
TTAGAAGAAT ACCTGAGTCA TCGGGAAATG AGCGAAAAGG CTCAGCAGGA AACAGGGGAA 
AATGAAACCT GCAATAGATA CAAAACTCCA CATCCTTCTG GCAGGAGTAA AAAGTGCTGC 
AATTCTATTT CTGTTGGAGC GTTTCTGGAT GATGATGACG ATGATGACAT GAGCTTAGAA 
GAAATTAAGA ACAGGCAAAA TGCAGCACGG AATATCAGCC AGCCTCTCAT GTCCAAAGAA 
CCCAGCATTG ATGCCATCGG AGATGCTGAG TGTGACATCT TGTCCATGGG GCATACAGTA 
AACATCATAG AAACATCAGT TCACCCCAGG CACTACGAAA ACGCAGCTTC TTCCAGCAAA 
AATGGATTTT GTAGTCCTGT GTCCAGTGAA AGGATCATCA GTGACAGAAA GCATCTGCAT 
GATGGAGAAG AATGCCTTGT AGCGCCTGTT TGCGATTTGA TGTCGGAATT TGAAAGCCTG 
TCATTCCAAG AACAAGACGT TCCCAGAGAA TCAAATAAAA TCACTGCAAA AACTCAGAAT 
GAGAGAATTC TGGCTGAAGG AACAAATCAG ATGAGAAAAT CCAAGGCCTG CAATGCAGTT 
TCACTGGCAT GTTCTTCTGA GTCAAGTGTT ACAGGCAAAC CTGGAGAGAC CAAGTTAAGG 
ACAGAACACA GAATACCACT AGAAAAGGAC AGACAGTCCA GGGAAGCTGG AATTCAGGCT 
AATGAAGCAC AACGTAAGGA ACTTTCTTCC CAGAAATTTC TCTCAAAGAC TTCACCCACA 
AATGACAATT CTAAGCTATT CCTGTTTGGG GAGCAGCCCT CAAAGCTGGA CAGTGATGTC 
TTAGCAGCGA TAGAAGCAGT AGAGGTTGAT CCACAGAAAT ACCCAACAGT CTACAAATGG 
AAACATACAG TGCAGTCGTA CTCTTCATCT GACAGGCAAA GTTGGCCAAG TCCAGCACTG 
AAGGCGAGAT TCAAGTCCCA AACTGTCACT GCTGGCCTTC CAGGTGCTTC TGTTTATCCC 
AATTCAGGAA GAAACAGTCC TGTGACAGGA AGTCCAGGAA AATACAGCAA CGCGGCCTCA 
TTCTCTCCAG ATCCTGGGAG TCCCGGGCGC TACAGTCCAG CTTGTGTCAA CCATGCTCTG 
CTGAAGCTGC GCTACTTTTC AGAGCCTCCT GCCCACTAA