LOC101871332 cDNA ORF clone, Melopsittacus undulatus(budgerigar)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101871332
Entrez Gene ID	101871332
Full Name	tolloid like 1
Synonyms	TLL1
Gene Type	protein-coding
Organism	Melopsittacus undulatus(budgerigar)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005145504.3	XP_005145561.1	tolloid-like protein 1
XM_005145504.2	XP_005145561.1	tolloid-like protein 1

Pathways from BioSystems
Gene Ontology

The following LOC101871332 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101871332 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe148443	XM_005145504.3 Latest version!	Melopsittacus undulatus tolloid like 1 (LOC101871332), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMe148443	XM_005145504.2	Melopsittacus undulatus tolloid-like 1 (LOC101871332), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101871332 ( XM_005145504.3 ) cDNA ORF clone, Melopsittacus undulatus(budgerigar) -> XP_005145561.1 Melopsittacus undulatus tolloid like 1 (LOC101871332), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe148443
Clone ID Related Accession (Same CDS sequence)	XM_005145504.3 , XM_005145504.2
Accession Version	XM_005145504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-03
Organism	Melopsittacus undulatus(budgerigar)
Product	tolloid-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004848226.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 4, 2019 this sequence version replaced XM_005145504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Melopsittacus undulatus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC TTTGCTGGAG GATGGCACTG TGGCTGGCCG GGTGGACTGT CTGTGGGAAG 
CCTTCCTCCT GTTTTGGCCT TGATTATGAC TACACTTATG ATTTCACTGA AGAGGATAAA 
GCTGAGGGGA TAGATTATAA GGATCCTTGC AAAGCCGCTG TCTTTTGGGG AGATATTGCC 
TTAGATGATG AAGACTTAAA AATCTTTCAA ATTGACAGGA CAATTGATCT TACGCAGCAC 
TCCAACGAAA GACTTGGCCA TAACACAGGT GGCTTTGGAG AGCATGGAAA GTCAAAAAAA 
AGAGGTGCCC TCTATCAGCT TATAGACAGG ATAAGAAGAT TTGGCTCTGG CTTTGAGCAA 
AATAATACAT CTAAAGGAAG AACTACTGTA AAATTCTCAG GGAAAAATGA GAAAAACAGA 
TTTCCCAGAG CTGCTACATC ACGAACAGAA AGAATATGGC CAGGGGGTGT CATTCCATAT 
GTAATAGGTG GAAATTTCAC TGGCACCCAG CGCGCCATGT TCAAACAGGC GATGCGGCAC 
TGGGAAAAGT ACACGTGCGT TACATTTATT GAACGAAGTG ATGAAGAAAG TTATATTGTA 
TTTACATACA GACCATGTGG GTGCTGTTCT TACGTGGGTC GAAGGGGAAA TGGCCCTCAG 
GCTATATCTA TTGGCAAGAA CTGTGATAAA TTTGGTATAG TTGTCCATGA GTTGGGTCAC 
GTGATAGGTT TTTGGCACGA ACATACACGA CCAGACCGTG ATGACCATGT CACCATCATT 
AGAGAAAATA TCCAGCCTGG TCAAGAATAC AACTTCCTGA AGATGGAGCC TGGAGAGGTG 
AACTCCCTTG GGGAACCGTA TGACTTTGAC AGCATAATGC ACTATGCCAG GAACACCTTC 
TCAAGGGGCA TGTTTCTGGA TACCATTCTC CCTTCCCGTG ATGATAATGG TATACGACCT 
GCCATTGGCC AGCGTACTCG TCTAAGTAAA GGAGATATTG CACAGGCAAG AAAGCTGTAT 
AGATGTCCAG CCTGTGGAGA AACACTGCAG GAATCTACAG GCAACTTTTC TTCTCCAGGA 
TTTCCAAATG GTTACCCTTC CTACACACAC TGCATATGGA GAATCTCTGT GACCCCAGGG 
GAGAAGATTG TTTTAAACTT CACCACCATG GACCTTTACA AGAGCAGTCT CTGTTGGTAC 
GATTATATTG AAGTAAGAGA TGGCTACTGG AGAAAATCAC CTCTGCTTGG CAGGTTTTGT 
GGTGACAAAC TGCCAGAAGT CCTCGCATCC TCGGACAGTA GGATGTGGAT CGAGTTCCGC 
AGCAGCAGCA ACTGGGTTGG GAAAGGTTTT GCAGCAATCT ATGAAGCTAT CTGTGGAGGT 
GAAATCCACA AAAATGAAGG CCAGATCCAG TCTCCCAATT ATCCTGATGA CTACAGACCA 
ATGAAGGAAT GTGTGTGGAA AATAACAGTA TCAGAAAACT ACAATGTTGG ATTAACCTTT 
CAAGCATTTG AGATTGAAAG ACACGATAAC TGTGCATATG ACTACTTGGA AATTCGAGAT 
GGAACAAATG AAAACAGTCC TTTAATTGGT CACTTTTGTG GCTATGACAA ACCAGAAGAC 
ATTAGATCTA CCTCTAATAC CCTGTGGATG AAGTTTGTTT CTGATGGAAC TGTCAACAAA 
GCAGGGTTTG CAGCTAACTT TTTTAAAGAG GAAGATGAAT GTGCCAAACC TGACAATGGT 
GGCTGTGAGC AGCGCTGTGT AAATACTCTG GGCAGTTACC AGTGCGCCTG TGATCCTGGT 
TATGAACTTG GTCCTGACAA AAAGAGCTGT GAAGCTGCTT GTGGAGGACT CCTGACAAAG 
CTAAATGGGA CTATAACCAC ACCAGGGTGG CCCAAAGAGT ATCCTCCAAA CAAAAACTGT 
GTGTGGCAGG TGGTTGCCCC AACGCAATAC CGAATTTCAA TGAAGTTTGA GTTTTTTGAG 
TTAGAAGGGA ATGAAGTTTG CAAGTATGAT TATGTGGAGA TTTGTAGTGG CCTTTCTTCT 
GATTCTAAAC TCCATGGTAA ATTCTGCGGT ACAGAAGTGC CTGAAGTTAT CACTTCTCAG 
TACAACAACA TGCGAATTGA GTTCAGATCT GACAACACTG TGTCAAAAAA AGGCTTCAAA 
GCACACTTCT TCTCAGACAA GGATGAGTGT TCAAAGGACA ATGGTGGCTG CCAGCATGAG 
TGCATCAACA CCGTAGGAAG CTATGTTTGC CAGTGCCGAA ATGGATTTGT GCTACATGAA 
AACAAACATG ACTGCAAGGA GGCTGAATGT GAACAGAAGA TCCATAGTCC CAATGGCATC 
ATCACAAGTC CCAACTGGCC CGACAAGTAT CCCAGCAGAA AGGAGTGCAC ATGGGAAATC 
AGTGCCACCC CAGGGCAGAG GGTCAAGTTG ACCTTTAATG AATTTGAGAT AGAACAACAT 
CAAGAATGTG CTTATGACCA CTTGGAAGTG TTTGATGGTG AAAGCGAAAA AGCACCAATT 
CTGGGACGTT TATGTGGCAA TAAGATACCA GATCCTCTTA TTGCTACTGG AAACAAAATG 
TTTCTCCGCT TTGTTTCTGA TGCATCGGTT CAAAGAAAAG GCTTCCAAGC AACACACTCT 
ACAGAATGTG GTGGTCGGCT GAAAGCTGAA ACCAAGCCCA AAGATCTGTA CTCGCATGCT 
CAGTTTGGCG ATAACAACTA TCCTGTCCAG GCAGACTGTG ACTGGCTATT GGTGGCAGAG 
CGCAGCTATA GAGTTGAGTT AATGTTTCAG ACGTTCGAGG TTGAAGAAGA GGCAGACTGT 
GGTTACGATT ATGTGGAACT CTTTGATGGT CATGATAAGA CAGCTGTGAG ACTTGGTCGA 
TTTTGTGGAT CTGGGCCACC AGAAGAAATC TATTCAGCAG GAGAAGCTCT TTTACTTCAC 
TTTCACACAG ATGATACAAT CAACAAGAAA GGATTTCATA TACGATACAG AAGTATAAAA 
TATCCAGATA GTGTGCACAC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005145561.1
CDS	493..3522
Translation	MKMLCWRMALWLAGWTVCGKPSSCFGLDYDYTYDFTEEDKAEGIDYKDPCKAAVFWGDIALDDEDLKIFQIDRTIDLTQHSNERLGHNTGGFGEHGKSKKRGALYQLIDRIRRFGSGFEQNNTSKGRTTVKFSGKNEKNRFPRAATSRTERIWPGGVIPYVIGGNFTGTQRAMFKQAMRHWEKYTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGEPYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLASSDSRMWIEFRSSSNWVGKGFAAIYEAICGGEIHKNEGQIQSPNYPDDYRPMKECVWKITVSENYNVGLTFQAFEIERHDNCAYDYLEIRDGTNENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDNGGCEQRCVNTLGSYQCACDPGYELGPDKKSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISMKFEFFELEGNEVCKYDYVEICSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFRSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECINTVGSYVCQCRNGFVLHENKHDCKEAECEQKIHSPNGIITSPNWPDKYPSRKECTWEISATPGQRVKLTFNEFEIEQHQECAYDHLEVFDGESEKAPILGRLCGNKIPDPLIATGNKMFLRFVSDASVQRKGFQATHSTECGGRLKAETKPKDLYSHAQFGDNNYPVQADCDWLLVAERSYRVELMFQTFEVEEEADCGYDYVELFDGHDKTAVRLGRFCGSGPPEEIYSAGEALLLHFHTDDTINKKGFHIRYRSIKYPDSVHTKK

Target ORF information:

RefSeq Version	XM_005145504.3
Organism	Melopsittacus undulatus(budgerigar)
Definition	Melopsittacus undulatus tolloid like 1 (LOC101871332), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005145504.3

ORF Insert Sequence:

ATGAAAATGC TTTGCTGGAG GATGGCACTG TGGCTGGCCG GGTGGACTGT CTGTGGGAAG 
CCTTCCTCCT GTTTTGGCCT TGATTATGAC TACACTTATG ATTTCACTGA AGAGGATAAA 
GCTGAGGGGA TAGATTATAA GGATCCTTGC AAAGCCGCTG TCTTTTGGGG AGATATTGCC 
TTAGATGATG AAGACTTAAA AATCTTTCAA ATTGACAGGA CAATTGATCT TACGCAGCAC 
TCCAACGAAA GACTTGGCCA TAACACAGGT GGCTTTGGAG AGCATGGAAA GTCAAAAAAA 
AGAGGTGCCC TCTATCAGCT TATAGACAGG ATAAGAAGAT TTGGCTCTGG CTTTGAGCAA 
AATAATACAT CTAAAGGAAG AACTACTGTA AAATTCTCAG GGAAAAATGA GAAAAACAGA 
TTTCCCAGAG CTGCTACATC ACGAACAGAA AGAATATGGC CAGGGGGTGT CATTCCATAT 
GTAATAGGTG GAAATTTCAC TGGCACCCAG CGCGCCATGT TCAAACAGGC GATGCGGCAC 
TGGGAAAAGT ACACGTGCGT TACATTTATT GAACGAAGTG ATGAAGAAAG TTATATTGTA 
TTTACATACA GACCATGTGG GTGCTGTTCT TACGTGGGTC GAAGGGGAAA TGGCCCTCAG 
GCTATATCTA TTGGCAAGAA CTGTGATAAA TTTGGTATAG TTGTCCATGA GTTGGGTCAC 
GTGATAGGTT TTTGGCACGA ACATACACGA CCAGACCGTG ATGACCATGT CACCATCATT 
AGAGAAAATA TCCAGCCTGG TCAAGAATAC AACTTCCTGA AGATGGAGCC TGGAGAGGTG 
AACTCCCTTG GGGAACCGTA TGACTTTGAC AGCATAATGC ACTATGCCAG GAACACCTTC 
TCAAGGGGCA TGTTTCTGGA TACCATTCTC CCTTCCCGTG ATGATAATGG TATACGACCT 
GCCATTGGCC AGCGTACTCG TCTAAGTAAA GGAGATATTG CACAGGCAAG AAAGCTGTAT 
AGATGTCCAG CCTGTGGAGA AACACTGCAG GAATCTACAG GCAACTTTTC TTCTCCAGGA 
TTTCCAAATG GTTACCCTTC CTACACACAC TGCATATGGA GAATCTCTGT GACCCCAGGG 
GAGAAGATTG TTTTAAACTT CACCACCATG GACCTTTACA AGAGCAGTCT CTGTTGGTAC 
GATTATATTG AAGTAAGAGA TGGCTACTGG AGAAAATCAC CTCTGCTTGG CAGGTTTTGT 
GGTGACAAAC TGCCAGAAGT CCTCGCATCC TCGGACAGTA GGATGTGGAT CGAGTTCCGC 
AGCAGCAGCA ACTGGGTTGG GAAAGGTTTT GCAGCAATCT ATGAAGCTAT CTGTGGAGGT 
GAAATCCACA AAAATGAAGG CCAGATCCAG TCTCCCAATT ATCCTGATGA CTACAGACCA 
ATGAAGGAAT GTGTGTGGAA AATAACAGTA TCAGAAAACT ACAATGTTGG ATTAACCTTT 
CAAGCATTTG AGATTGAAAG ACACGATAAC TGTGCATATG ACTACTTGGA AATTCGAGAT 
GGAACAAATG AAAACAGTCC TTTAATTGGT CACTTTTGTG GCTATGACAA ACCAGAAGAC 
ATTAGATCTA CCTCTAATAC CCTGTGGATG AAGTTTGTTT CTGATGGAAC TGTCAACAAA 
GCAGGGTTTG CAGCTAACTT TTTTAAAGAG GAAGATGAAT GTGCCAAACC TGACAATGGT 
GGCTGTGAGC AGCGCTGTGT AAATACTCTG GGCAGTTACC AGTGCGCCTG TGATCCTGGT 
TATGAACTTG GTCCTGACAA AAAGAGCTGT GAAGCTGCTT GTGGAGGACT CCTGACAAAG 
CTAAATGGGA CTATAACCAC ACCAGGGTGG CCCAAAGAGT ATCCTCCAAA CAAAAACTGT 
GTGTGGCAGG TGGTTGCCCC AACGCAATAC CGAATTTCAA TGAAGTTTGA GTTTTTTGAG 
TTAGAAGGGA ATGAAGTTTG CAAGTATGAT TATGTGGAGA TTTGTAGTGG CCTTTCTTCT 
GATTCTAAAC TCCATGGTAA ATTCTGCGGT ACAGAAGTGC CTGAAGTTAT CACTTCTCAG 
TACAACAACA TGCGAATTGA GTTCAGATCT GACAACACTG TGTCAAAAAA AGGCTTCAAA 
GCACACTTCT TCTCAGACAA GGATGAGTGT TCAAAGGACA ATGGTGGCTG CCAGCATGAG 
TGCATCAACA CCGTAGGAAG CTATGTTTGC CAGTGCCGAA ATGGATTTGT GCTACATGAA 
AACAAACATG ACTGCAAGGA GGCTGAATGT GAACAGAAGA TCCATAGTCC CAATGGCATC 
ATCACAAGTC CCAACTGGCC CGACAAGTAT CCCAGCAGAA AGGAGTGCAC ATGGGAAATC 
AGTGCCACCC CAGGGCAGAG GGTCAAGTTG ACCTTTAATG AATTTGAGAT AGAACAACAT 
CAAGAATGTG CTTATGACCA CTTGGAAGTG TTTGATGGTG AAAGCGAAAA AGCACCAATT 
CTGGGACGTT TATGTGGCAA TAAGATACCA GATCCTCTTA TTGCTACTGG AAACAAAATG 
TTTCTCCGCT TTGTTTCTGA TGCATCGGTT CAAAGAAAAG GCTTCCAAGC AACACACTCT 
ACAGAATGTG GTGGTCGGCT GAAAGCTGAA ACCAAGCCCA AAGATCTGTA CTCGCATGCT 
CAGTTTGGCG ATAACAACTA TCCTGTCCAG GCAGACTGTG ACTGGCTATT GGTGGCAGAG 
CGCAGCTATA GAGTTGAGTT AATGTTTCAG ACGTTCGAGG TTGAAGAAGA GGCAGACTGT 
GGTTACGATT ATGTGGAACT CTTTGATGGT CATGATAAGA CAGCTGTGAG ACTTGGTCGA 
TTTTGTGGAT CTGGGCCACC AGAAGAAATC TATTCAGCAG GAGAAGCTCT TTTACTTCAC 
TTTCACACAG ATGATACAAT CAACAAGAAA GGATTTCATA TACGATACAG AAGTATAAAA 
TATCCAGATA GTGTGCACAC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101871332 ( XM_005145504.2 ) cDNA ORF clone, Melopsittacus undulatus(budgerigar) -> XP_005145561.1 Melopsittacus undulatus tolloid-like 1 (LOC101871332), mRNA.

Price & Availability↑

CloneID	OMe148443
Clone ID Related Accession (Same CDS sequence)	XM_005145504.3 , XM_005145504.2
Accession Version	XM_005145504.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-13
Organism	Melopsittacus undulatus(budgerigar)
Product	tolloid-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004848226.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 14, 2015 this sequence version replaced XM_005145504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Melopsittacus undulatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC TTTGCTGGAG GATGGCACTG TGGCTGGCCG GGTGGACTGT CTGTGGGAAG 
CCTTCCTCCT GTTTTGGCCT TGATTATGAC TACACTTATG ATTTCACTGA AGAGGATAAA 
GCTGAGGGGA TAGATTATAA GGATCCTTGC AAAGCCGCTG TCTTTTGGGG AGATATTGCC 
TTAGATGATG AAGACTTAAA AATCTTTCAA ATTGACAGGA CAATTGATCT TACGCAGCAC 
TCCAACGAAA GACTTGGCCA TAACACAGGT GGCTTTGGAG AGCATGGAAA GTCAAAAAAA 
AGAGGTGCCC TCTATCAGCT TATAGACAGG ATAAGAAGAT TTGGCTCTGG CTTTGAGCAA 
AATAATACAT CTAAAGGAAG AACTACTGTA AAATTCTCAG GGAAAAATGA GAAAAACAGA 
TTTCCCAGAG CTGCTACATC ACGAACAGAA AGAATATGGC CAGGGGGTGT CATTCCATAT 
GTAATAGGTG GAAATTTCAC TGGCACCCAG CGCGCCATGT TCAAACAGGC GATGCGGCAC 
TGGGAAAAGT ACACGTGCGT TACATTTATT GAACGAAGTG ATGAAGAAAG TTATATTGTA 
TTTACATACA GACCATGTGG GTGCTGTTCT TACGTGGGTC GAAGGGGAAA TGGCCCTCAG 
GCTATATCTA TTGGCAAGAA CTGTGATAAA TTTGGTATAG TTGTCCATGA GTTGGGTCAC 
GTGATAGGTT TTTGGCACGA ACATACACGA CCAGACCGTG ATGACCATGT CACCATCATT 
AGAGAAAATA TCCAGCCTGG TCAAGAATAC AACTTCCTGA AGATGGAGCC TGGAGAGGTG 
AACTCCCTTG GGGAACCGTA TGACTTTGAC AGCATAATGC ACTATGCCAG GAACACCTTC 
TCAAGGGGCA TGTTTCTGGA TACCATTCTC CCTTCCCGTG ATGATAATGG TATACGACCT 
GCCATTGGCC AGCGTACTCG TCTAAGTAAA GGAGATATTG CACAGGCAAG AAAGCTGTAT 
AGATGTCCAG CCTGTGGAGA AACACTGCAG GAATCTACAG GCAACTTTTC TTCTCCAGGA 
TTTCCAAATG GTTACCCTTC CTACACACAC TGCATATGGA GAATCTCTGT GACCCCAGGG 
GAGAAGATTG TTTTAAACTT CACCACCATG GACCTTTACA AGAGCAGTCT CTGTTGGTAC 
GATTATATTG AAGTAAGAGA TGGCTACTGG AGAAAATCAC CTCTGCTTGG CAGGTTTTGT 
GGTGACAAAC TGCCAGAAGT CCTCGCATCC TCGGACAGTA GGATGTGGAT CGAGTTCCGC 
AGCAGCAGCA ACTGGGTTGG GAAAGGTTTT GCAGCAATCT ATGAAGCTAT CTGTGGAGGT 
GAAATCCACA AAAATGAAGG CCAGATCCAG TCTCCCAATT ATCCTGATGA CTACAGACCA 
ATGAAGGAAT GTGTGTGGAA AATAACAGTA TCAGAAAACT ACAATGTTGG ATTAACCTTT 
CAAGCATTTG AGATTGAAAG ACACGATAAC TGTGCATATG ACTACTTGGA AATTCGAGAT 
GGAACAAATG AAAACAGTCC TTTAATTGGT CACTTTTGTG GCTATGACAA ACCAGAAGAC 
ATTAGATCTA CCTCTAATAC CCTGTGGATG AAGTTTGTTT CTGATGGAAC TGTCAACAAA 
GCAGGGTTTG CAGCTAACTT TTTTAAAGAG GAAGATGAAT GTGCCAAACC TGACAATGGT 
GGCTGTGAGC AGCGCTGTGT AAATACTCTG GGCAGTTACC AGTGCGCCTG TGATCCTGGT 
TATGAACTTG GTCCTGACAA AAAGAGCTGT GAAGCTGCTT GTGGAGGACT CCTGACAAAG 
CTAAATGGGA CTATAACCAC ACCAGGGTGG CCCAAAGAGT ATCCTCCAAA CAAAAACTGT 
GTGTGGCAGG TGGTTGCCCC AACGCAATAC CGAATTTCAA TGAAGTTTGA GTTTTTTGAG 
TTAGAAGGGA ATGAAGTTTG CAAGTATGAT TATGTGGAGA TTTGTAGTGG CCTTTCTTCT 
GATTCTAAAC TCCATGGTAA ATTCTGCGGT ACAGAAGTGC CTGAAGTTAT CACTTCTCAG 
TACAACAACA TGCGAATTGA GTTCAGATCT GACAACACTG TGTCAAAAAA AGGCTTCAAA 
GCACACTTCT TCTCAGACAA GGATGAGTGT TCAAAGGACA ATGGTGGCTG CCAGCATGAG 
TGCATCAACA CCGTAGGAAG CTATGTTTGC CAGTGCCGAA ATGGATTTGT GCTACATGAA 
AACAAACATG ACTGCAAGGA GGCTGAATGT GAACAGAAGA TCCATAGTCC CAATGGCATC 
ATCACAAGTC CCAACTGGCC CGACAAGTAT CCCAGCAGAA AGGAGTGCAC ATGGGAAATC 
AGTGCCACCC CAGGGCAGAG GGTCAAGTTG ACCTTTAATG AATTTGAGAT AGAACAACAT 
CAAGAATGTG CTTATGACCA CTTGGAAGTG TTTGATGGTG AAAGCGAAAA AGCACCAATT 
CTGGGACGTT TATGTGGCAA TAAGATACCA GATCCTCTTA TTGCTACTGG AAACAAAATG 
TTTCTCCGCT TTGTTTCTGA TGCATCGGTT CAAAGAAAAG GCTTCCAAGC AACACACTCT 
ACAGAATGTG GTGGTCGGCT GAAAGCTGAA ACCAAGCCCA AAGATCTGTA CTCGCATGCT 
CAGTTTGGCG ATAACAACTA TCCTGTCCAG GCAGACTGTG ACTGGCTATT GGTGGCAGAG 
CGCAGCTATA GAGTTGAGTT AATGTTTCAG ACGTTCGAGG TTGAAGAAGA GGCAGACTGT 
GGTTACGATT ATGTGGAACT CTTTGATGGT CATGATAAGA CAGCTGTGAG ACTTGGTCGA 
TTTTGTGGAT CTGGGCCACC AGAAGAAATC TATTCAGCAG GAGAAGCTCT TTTACTTCAC 
TTTCACACAG ATGATACAAT CAACAAGAAA GGATTTCATA TACGATACAG AAGTATAAAA 
TATCCAGATA GTGTGCACAC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005145561.1
CDS	1..3030
Translation	MKMLCWRMALWLAGWTVCGKPSSCFGLDYDYTYDFTEEDKAEGIDYKDPCKAAVFWGDIALDDEDLKIFQIDRTIDLTQHSNERLGHNTGGFGEHGKSKKRGALYQLIDRIRRFGSGFEQNNTSKGRTTVKFSGKNEKNRFPRAATSRTERIWPGGVIPYVIGGNFTGTQRAMFKQAMRHWEKYTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGEPYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLASSDSRMWIEFRSSSNWVGKGFAAIYEAICGGEIHKNEGQIQSPNYPDDYRPMKECVWKITVSENYNVGLTFQAFEIERHDNCAYDYLEIRDGTNENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDNGGCEQRCVNTLGSYQCACDPGYELGPDKKSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISMKFEFFELEGNEVCKYDYVEICSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFRSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECINTVGSYVCQCRNGFVLHENKHDCKEAECEQKIHSPNGIITSPNWPDKYPSRKECTWEISATPGQRVKLTFNEFEIEQHQECAYDHLEVFDGESEKAPILGRLCGNKIPDPLIATGNKMFLRFVSDASVQRKGFQATHSTECGGRLKAETKPKDLYSHAQFGDNNYPVQADCDWLLVAERSYRVELMFQTFEVEEEADCGYDYVELFDGHDKTAVRLGRFCGSGPPEEIYSAGEALLLHFHTDDTINKKGFHIRYRSIKYPDSVHTKK

Target ORF information:

RefSeq Version	XM_005145504.2
Organism	Melopsittacus undulatus(budgerigar)
Definition	Melopsittacus undulatus tolloid-like 1 (LOC101871332), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005145504.2

ORF Insert Sequence:

ATGAAAATGC TTTGCTGGAG GATGGCACTG TGGCTGGCCG GGTGGACTGT CTGTGGGAAG 
CCTTCCTCCT GTTTTGGCCT TGATTATGAC TACACTTATG ATTTCACTGA AGAGGATAAA 
GCTGAGGGGA TAGATTATAA GGATCCTTGC AAAGCCGCTG TCTTTTGGGG AGATATTGCC 
TTAGATGATG AAGACTTAAA AATCTTTCAA ATTGACAGGA CAATTGATCT TACGCAGCAC 
TCCAACGAAA GACTTGGCCA TAACACAGGT GGCTTTGGAG AGCATGGAAA GTCAAAAAAA 
AGAGGTGCCC TCTATCAGCT TATAGACAGG ATAAGAAGAT TTGGCTCTGG CTTTGAGCAA 
AATAATACAT CTAAAGGAAG AACTACTGTA AAATTCTCAG GGAAAAATGA GAAAAACAGA 
TTTCCCAGAG CTGCTACATC ACGAACAGAA AGAATATGGC CAGGGGGTGT CATTCCATAT 
GTAATAGGTG GAAATTTCAC TGGCACCCAG CGCGCCATGT TCAAACAGGC GATGCGGCAC 
TGGGAAAAGT ACACGTGCGT TACATTTATT GAACGAAGTG ATGAAGAAAG TTATATTGTA 
TTTACATACA GACCATGTGG GTGCTGTTCT TACGTGGGTC GAAGGGGAAA TGGCCCTCAG 
GCTATATCTA TTGGCAAGAA CTGTGATAAA TTTGGTATAG TTGTCCATGA GTTGGGTCAC 
GTGATAGGTT TTTGGCACGA ACATACACGA CCAGACCGTG ATGACCATGT CACCATCATT 
AGAGAAAATA TCCAGCCTGG TCAAGAATAC AACTTCCTGA AGATGGAGCC TGGAGAGGTG 
AACTCCCTTG GGGAACCGTA TGACTTTGAC AGCATAATGC ACTATGCCAG GAACACCTTC 
TCAAGGGGCA TGTTTCTGGA TACCATTCTC CCTTCCCGTG ATGATAATGG TATACGACCT 
GCCATTGGCC AGCGTACTCG TCTAAGTAAA GGAGATATTG CACAGGCAAG AAAGCTGTAT 
AGATGTCCAG CCTGTGGAGA AACACTGCAG GAATCTACAG GCAACTTTTC TTCTCCAGGA 
TTTCCAAATG GTTACCCTTC CTACACACAC TGCATATGGA GAATCTCTGT GACCCCAGGG 
GAGAAGATTG TTTTAAACTT CACCACCATG GACCTTTACA AGAGCAGTCT CTGTTGGTAC 
GATTATATTG AAGTAAGAGA TGGCTACTGG AGAAAATCAC CTCTGCTTGG CAGGTTTTGT 
GGTGACAAAC TGCCAGAAGT CCTCGCATCC TCGGACAGTA GGATGTGGAT CGAGTTCCGC 
AGCAGCAGCA ACTGGGTTGG GAAAGGTTTT GCAGCAATCT ATGAAGCTAT CTGTGGAGGT 
GAAATCCACA AAAATGAAGG CCAGATCCAG TCTCCCAATT ATCCTGATGA CTACAGACCA 
ATGAAGGAAT GTGTGTGGAA AATAACAGTA TCAGAAAACT ACAATGTTGG ATTAACCTTT 
CAAGCATTTG AGATTGAAAG ACACGATAAC TGTGCATATG ACTACTTGGA AATTCGAGAT 
GGAACAAATG AAAACAGTCC TTTAATTGGT CACTTTTGTG GCTATGACAA ACCAGAAGAC 
ATTAGATCTA CCTCTAATAC CCTGTGGATG AAGTTTGTTT CTGATGGAAC TGTCAACAAA 
GCAGGGTTTG CAGCTAACTT TTTTAAAGAG GAAGATGAAT GTGCCAAACC TGACAATGGT 
GGCTGTGAGC AGCGCTGTGT AAATACTCTG GGCAGTTACC AGTGCGCCTG TGATCCTGGT 
TATGAACTTG GTCCTGACAA AAAGAGCTGT GAAGCTGCTT GTGGAGGACT CCTGACAAAG 
CTAAATGGGA CTATAACCAC ACCAGGGTGG CCCAAAGAGT ATCCTCCAAA CAAAAACTGT 
GTGTGGCAGG TGGTTGCCCC AACGCAATAC CGAATTTCAA TGAAGTTTGA GTTTTTTGAG 
TTAGAAGGGA ATGAAGTTTG CAAGTATGAT TATGTGGAGA TTTGTAGTGG CCTTTCTTCT 
GATTCTAAAC TCCATGGTAA ATTCTGCGGT ACAGAAGTGC CTGAAGTTAT CACTTCTCAG 
TACAACAACA TGCGAATTGA GTTCAGATCT GACAACACTG TGTCAAAAAA AGGCTTCAAA 
GCACACTTCT TCTCAGACAA GGATGAGTGT TCAAAGGACA ATGGTGGCTG CCAGCATGAG 
TGCATCAACA CCGTAGGAAG CTATGTTTGC CAGTGCCGAA ATGGATTTGT GCTACATGAA 
AACAAACATG ACTGCAAGGA GGCTGAATGT GAACAGAAGA TCCATAGTCC CAATGGCATC 
ATCACAAGTC CCAACTGGCC CGACAAGTAT CCCAGCAGAA AGGAGTGCAC ATGGGAAATC 
AGTGCCACCC CAGGGCAGAG GGTCAAGTTG ACCTTTAATG AATTTGAGAT AGAACAACAT 
CAAGAATGTG CTTATGACCA CTTGGAAGTG TTTGATGGTG AAAGCGAAAA AGCACCAATT 
CTGGGACGTT TATGTGGCAA TAAGATACCA GATCCTCTTA TTGCTACTGG AAACAAAATG 
TTTCTCCGCT TTGTTTCTGA TGCATCGGTT CAAAGAAAAG GCTTCCAAGC AACACACTCT 
ACAGAATGTG GTGGTCGGCT GAAAGCTGAA ACCAAGCCCA AAGATCTGTA CTCGCATGCT 
CAGTTTGGCG ATAACAACTA TCCTGTCCAG GCAGACTGTG ACTGGCTATT GGTGGCAGAG 
CGCAGCTATA GAGTTGAGTT AATGTTTCAG ACGTTCGAGG TTGAAGAAGA GGCAGACTGT 
GGTTACGATT ATGTGGAACT CTTTGATGGT CATGATAAGA CAGCTGTGAG ACTTGGTCGA 
TTTTGTGGAT CTGGGCCACC AGAAGAAATC TATTCAGCAG GAGAAGCTCT TTTACTTCAC 
TTTCACACAG ATGATACAAT CAACAAGAAA GGATTTCATA TACGATACAG AAGTATAAAA 
TATCCAGATA GTGTGCACAC CAAAAAATAA