LOC101867810 cDNA ORF clone, Melopsittacus undulatus(budgerigar)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101867810
Entrez Gene ID	101867810
Full Name	ring finger and CCCH-type domains 2
Synonyms	RC3H2
Gene Type	protein-coding
Organism	Melopsittacus undulatus(budgerigar)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005146351.1	XP_005146408.1	LOW QUALITY PROTEIN: roquin-2
XM_031046428.1	XP_030902288.1	LOW QUALITY PROTEIN: roquin-2

Pathways from BioSystems
Gene Ontology

The following LOC101867810 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101867810 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe149194	XM_005146351.1 Latest version!	Melopsittacus undulatus ring finger and CCCH-type domains 2 (LOC101867810), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMe349033	XM_031046428.1 Latest version!	Melopsittacus undulatus ring finger and CCCH-type domains 2 (LOC101867810), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101867810 ( XM_005146351.1 ) cDNA ORF clone, Melopsittacus undulatus(budgerigar) -> XP_005146408.1 Melopsittacus undulatus ring finger and CCCH-type domains 2 (LOC101867810), mRNA.

Price & Availability↑

CloneID	OMe149194
Clone ID Related Accession (Same CDS sequence)	XM_005146351.1
Accession Version	XM_005146351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-13
Organism	Melopsittacus undulatus(budgerigar)
Product	LOW QUALITY PROTEIN: roquin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004848237.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Melopsittacus undulatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTGTGC AGGCAGCTCA GTGGACAGAA TTTCTGTCCT GCCCAATCTG CTACAACGAG 
TTTGATGAGA ATGTGCACAA ACCCATCAGC TTAGGTTGCT CTCACACTGT CTGTAAGACC 
TGCCTGAACA AGCTTCATCG CAAGGCATGT CCTTTCGACC AGACTGCCAT CAACACGGAC 
ATCGATGTTC TTCCTGTAAA CTTCGCACTC CTCCAGCTAG TTGGAGCCCA GGTACCCGAT 
CATCAGACAG TAAAGTTGAG TAATGTAGGA GAGAACAAAC ATTATGAAGT AGCAAAGAAA 
TGTGTTGAGG ATTTGGCACT CTACTTAAAG CCATTAAGTG GAGGGAAAGG TGTTGCAAGC 
TTGAATCAGA GTGCACTGAG CCGTCCAATG CAAAGGAAGC TTGTGACACT GGTGAATTGT 
CAGCTGGTGG AGGAAGAGGG TCGGGTCAGA GCTATGAGAG CAGCTCGATC ACTGGGAGAG 
AGAACTGTCA CAGAACTGAT CTTGCAGCAC CAAAATCCTC AGCAGCTTTC TGCCAATCTT 
TGGGCTGCTG TCAGGGCACG AGGATGCCAG TTTCTAGGAC CAGCTATGCA AGAGGAGGCA 
CTGAAACTTG TATTACTGGC ACTGGAAGAC GGCTCTGCAC TCTCAAGAAA AGTTTTGGTA 
CTTTTTGTTG TGCAAAGGCT AGAACCAAGA TTTCCTCAGG CCTCTAAAAC AAGCATTGGT 
CATGTTGTGC AGCTACTGTA TAGAGCATCT TGCTTTAAGG TCACTAAAAG GGATGAAGAT 
TCTTCTCTGA TGCAGCTTAA AGAAGAGTTT CGGAGTTATG AGGCCTTGCG GAGAGAACAT 
GATGCCCAAA TTGTTCACAT TGCCATGGAA GCAGGACTTC GAATATCACC AGAACAATGG 
TCTTCTCTTC TGTATGGTGA CTTGGCACAT AAATCACACA TGCAATCCAT TATTGACAAG 
CCACAACCAA ATAGCAAATA CAAAACAAGT ATGTGCCGAG ACCTTCGACA GCAAGGGGGA 
TGTCCAAGAG GAACAAACTG TACATTTGCT CATTCTCAGG AAGAGCTCGA AAAATACCGC 
TTGAGGAACA AAAAAATCAG TGCAACAGTG AGAACATTCC CCCTTCTAAA TAAAGTTGGC 
GTAAATAGCA CCGTCTCAAC GACAACAGGA AACGTCATTT CTGTCATAGG AAGCCCTGAA 
GCAACAGGGA AGATGGTGCC AAGTACTAAC GGAATAGCTA ATCTGGAAAG TGGGGTTCCC 
CAGTTGATCC CTCGCTGTGC AGACACCTCC TTGAGAGCTT TGGAGAACAC CAAGAAGGGA 
GGGAAAACTG GAGCAAATGG CCAGAACGTT TCTGGATCCC CTACAGAATC ACTACCTGAA 
AATAAAATTG GTCTCCACCC AAGACTCCTG NNAAGCCAGG CAGCAGCTAC CTCAGCTGGT 
CCTCCTAATA TCGGAACAGA AGTTAATTCT GTGCCTCCAA AATCCAGCCC GTTTGTTCCC 
AGAGTACCTG TCTACCCTCC ACATTCTGAT AATGTTCAAT ATTTCCAAGA TCCCCGGACT 
CAGCTGTCAT ATGAAGTTCC ACAGTACCCA CAGACAGGAT ATTATCCACC ACCTCCAACA 
GTACCAGCTG GTGTGGCTCC CTGTGTTCCT CGCTTTGTGA GGTCCAATAA CGTTCCAGAG 
TCCTCCCTCC CACCTGCTTC CGTGCCATAT GCCGATCATT ACAGTACATT TCCCCCTCGA 
GATCGACTGA ATTCTCCTTA CCAACCTCCT CCTCCGCAGC CGTATGGACC AGTTCCTCCT 
GTCCCTTCTG GAATGTATGC TCCAGTTTAT GACAGCAGGC GCATCTGGCG CCCACAGATG 
TACCCACGAG ATGATATTAT TAGGAGCAAT TCTTTACCTC CCATGGATGT GATGCACTCA 
TCTGTCTATC AGACATCATT ACGTGAGAGA TACAACTCTT TGGATGGGTA TTACTCTGTG 
GCTTGTCAAC CTCCAAACGA GCAGAGGACT GTGCCTTTAC CAAGGGAGCC TTGTGGTCAT 
TTGAAGACTG GTTATGAAGA GCAGTTAAGA CGGAAGCCAG AGCAATGGGC ACAGTACCAC 
ACGCAGAAAA CTTCTCTGGT ATCGTCAACC CTTCCTATGG CAACACCATC TCCAACACCA 
CCTTCTCCTC TCTTCAGCGT AGATTTCAGC ACAGAGTTCT CAGAAAATGT CAGTGATTTG 
AGTGGAACTA AATTTGAGGA AGACCATCTC TCTCACTATT CACCGTGGTC TTGTGGCACT 
ATTGGCTCTT GTATAAATGC TATCGACTCA GAGCCCAAGG ATGTGATTGC CAATTCAAAT 
GCCGTGTTAA TGGATTTGGA CAGCGGGGAT GTTAAAAGGA GAGTGCATTT ATTTGAAACT 
CAGAGAAGGG CAAAGGAAGA AGATCCTATA ATCCCATTTA GCGATGGACC GATCATCTCC 
AAGTGGGGTG CAATCTCCAG GTCATCCCGC ACCGGTTATC ACACGACAGA TCCTATTCAG 
GCCACTGCTT CCCAAGGAAG TGCTACTAAA CCCATCAGTG TATCAGATTA TGTCCCTTAT 
ATCAATGCTG TTGATTCAAG ATGGAGTGCC TATGGCTCAG ATTCTGCTTC ATCAGCACGT 
TATGCAGAAC GGGACAGGTT CATAGTTGCA GATTTGTCTG GTCACCGAAA GCATTCCAGC 
ACTGGAGATC TATTGAGTAT TGAATTACAG CAGGCCAAAA GTAACTCATT ATTACTTCAG 
AGAGAGGCAA ATGCACTAGC CATGCAACAG AAGTGGAATT CTCTAGATGA AGGCAGTCGT 
CTTACCTTAA ATCTTTTAAG CAAGGAAATT GATTTGAGGA ATGGTGAGGT AAAGAAAGGG 
ACTGATTATA CTGAAGACTG TGCAGACACG AAGCCAGATC GAGACATTGA ACTGGAGCTG 
TCAGCCCTGG ATACAGATGA ACCTGATGGG CAAGGTGAAC AAATAGAAGA GATTCTGGAT 
ATACAGCTAG GTATTAGTTC TCAAGATGAT CAGCTGCTCA ATGGGACAAC TGTAGAGAAT 
GGACATCTGC TAAAGCAGCA CCAGAAAGAA TCTATGGAAC AGAAGAGACA AAGTTTAGGT 
GAAGACCTTG TGATTCTGGA GGAGCAGAAA ACAATCCTGC CCGTAACTTC TTGCTTCAGT 
CAGCCGATCA CATCTGTTAG CAATGCAAGC TGCCTGCCCA TCAGCACAGC AGTCAGTGTT 
GGCAGCCTCA TTTTGAAAAC TGCTCACATT ATGTCTGAGG ATAAAAATGA CTTTTTAAAG 
CCTGTTGCAA GTGGCAGGAT GGTTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005146408.1
CDS	32..3364
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQTVKLSNVGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKISATVRTFPLLNKVGVNSTVSTTTGNVISVIGSPEATGKMVPSTNGIANLESGVPQLIPRCADTSLRALENTKKGGKTGANGQNVSGSPTESLPENKIGLHPRLLnullSQAAATSAGPPNIGTEVNSVPPKSSPFVPRVPVYPPHSDNVQYFQDPRTQLSYEVPQYPQTGYYPPPPTVPAGVAPCVPRFVRSNNVPESSLPPASVPYADHYSTFPPRDRLNSPYQPPPPQPYGPVPPVPSGMYAPVYDSRRIWRPQMYPRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPNEQRTVPLPREPCGHLKTGYEEQLRRKPEQWAQYHTQKTSLVSSTLPMATPSPTPPSPLFSVDFSTEFSENVSDLSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFETQRRAKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPIQATASQGSATKPISVSDYVPYINAVDSRWSAYGSDSASSARYAERDRFIVADLSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGSRLTLNLLSKEIDLRNGEVKKGTDYTEDCADTKPDRDIELELSALDTDEPDGQGEQIEEILDIQLGISSQDDQLLNGTTVENGHLLKQHQKESMEQKRQSLGEDLVILEEQKTILPVTSCFSQPITSVSNASCLPISTAVSVGSLILKTAHIMSEDKNDFLKPVASGRMVNS

Target ORF information:

RefSeq Version	XM_005146351.1
Organism	Melopsittacus undulatus(budgerigar)
Definition	Melopsittacus undulatus ring finger and CCCH-type domains 2 (LOC101867810), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005146351.1

ORF Insert Sequence:

ATGCCTGTGC AGGCAGCTCA GTGGACAGAA TTTCTGTCCT GCCCAATCTG CTACAACGAG 
TTTGATGAGA ATGTGCACAA ACCCATCAGC TTAGGTTGCT CTCACACTGT CTGTAAGACC 
TGCCTGAACA AGCTTCATCG CAAGGCATGT CCTTTCGACC AGACTGCCAT CAACACGGAC 
ATCGATGTTC TTCCTGTAAA CTTCGCACTC CTCCAGCTAG TTGGAGCCCA GGTACCCGAT 
CATCAGACAG TAAAGTTGAG TAATGTAGGA GAGAACAAAC ATTATGAAGT AGCAAAGAAA 
TGTGTTGAGG ATTTGGCACT CTACTTAAAG CCATTAAGTG GAGGGAAAGG TGTTGCAAGC 
TTGAATCAGA GTGCACTGAG CCGTCCAATG CAAAGGAAGC TTGTGACACT GGTGAATTGT 
CAGCTGGTGG AGGAAGAGGG TCGGGTCAGA GCTATGAGAG CAGCTCGATC ACTGGGAGAG 
AGAACTGTCA CAGAACTGAT CTTGCAGCAC CAAAATCCTC AGCAGCTTTC TGCCAATCTT 
TGGGCTGCTG TCAGGGCACG AGGATGCCAG TTTCTAGGAC CAGCTATGCA AGAGGAGGCA 
CTGAAACTTG TATTACTGGC ACTGGAAGAC GGCTCTGCAC TCTCAAGAAA AGTTTTGGTA 
CTTTTTGTTG TGCAAAGGCT AGAACCAAGA TTTCCTCAGG CCTCTAAAAC AAGCATTGGT 
CATGTTGTGC AGCTACTGTA TAGAGCATCT TGCTTTAAGG TCACTAAAAG GGATGAAGAT 
TCTTCTCTGA TGCAGCTTAA AGAAGAGTTT CGGAGTTATG AGGCCTTGCG GAGAGAACAT 
GATGCCCAAA TTGTTCACAT TGCCATGGAA GCAGGACTTC GAATATCACC AGAACAATGG 
TCTTCTCTTC TGTATGGTGA CTTGGCACAT AAATCACACA TGCAATCCAT TATTGACAAG 
CCACAACCAA ATAGCAAATA CAAAACAAGT ATGTGCCGAG ACCTTCGACA GCAAGGGGGA 
TGTCCAAGAG GAACAAACTG TACATTTGCT CATTCTCAGG AAGAGCTCGA AAAATACCGC 
TTGAGGAACA AAAAAATCAG TGCAACAGTG AGAACATTCC CCCTTCTAAA TAAAGTTGGC 
GTAAATAGCA CCGTCTCAAC GACAACAGGA AACGTCATTT CTGTCATAGG AAGCCCTGAA 
GCAACAGGGA AGATGGTGCC AAGTACTAAC GGAATAGCTA ATCTGGAAAG TGGGGTTCCC 
CAGTTGATCC CTCGCTGTGC AGACACCTCC TTGAGAGCTT TGGAGAACAC CAAGAAGGGA 
GGGAAAACTG GAGCAAATGG CCAGAACGTT TCTGGATCCC CTACAGAATC ACTACCTGAA 
AATAAAATTG GTCTCCACCC AAGACTCCTG NNAAGCCAGG CAGCAGCTAC CTCAGCTGGT 
CCTCCTAATA TCGGAACAGA AGTTAATTCT GTGCCTCCAA AATCCAGCCC GTTTGTTCCC 
AGAGTACCTG TCTACCCTCC ACATTCTGAT AATGTTCAAT ATTTCCAAGA TCCCCGGACT 
CAGCTGTCAT ATGAAGTTCC ACAGTACCCA CAGACAGGAT ATTATCCACC ACCTCCAACA 
GTACCAGCTG GTGTGGCTCC CTGTGTTCCT CGCTTTGTGA GGTCCAATAA CGTTCCAGAG 
TCCTCCCTCC CACCTGCTTC CGTGCCATAT GCCGATCATT ACAGTACATT TCCCCCTCGA 
GATCGACTGA ATTCTCCTTA CCAACCTCCT CCTCCGCAGC CGTATGGACC AGTTCCTCCT 
GTCCCTTCTG GAATGTATGC TCCAGTTTAT GACAGCAGGC GCATCTGGCG CCCACAGATG 
TACCCACGAG ATGATATTAT TAGGAGCAAT TCTTTACCTC CCATGGATGT GATGCACTCA 
TCTGTCTATC AGACATCATT ACGTGAGAGA TACAACTCTT TGGATGGGTA TTACTCTGTG 
GCTTGTCAAC CTCCAAACGA GCAGAGGACT GTGCCTTTAC CAAGGGAGCC TTGTGGTCAT 
TTGAAGACTG GTTATGAAGA GCAGTTAAGA CGGAAGCCAG AGCAATGGGC ACAGTACCAC 
ACGCAGAAAA CTTCTCTGGT ATCGTCAACC CTTCCTATGG CAACACCATC TCCAACACCA 
CCTTCTCCTC TCTTCAGCGT AGATTTCAGC ACAGAGTTCT CAGAAAATGT CAGTGATTTG 
AGTGGAACTA AATTTGAGGA AGACCATCTC TCTCACTATT CACCGTGGTC TTGTGGCACT 
ATTGGCTCTT GTATAAATGC TATCGACTCA GAGCCCAAGG ATGTGATTGC CAATTCAAAT 
GCCGTGTTAA TGGATTTGGA CAGCGGGGAT GTTAAAAGGA GAGTGCATTT ATTTGAAACT 
CAGAGAAGGG CAAAGGAAGA AGATCCTATA ATCCCATTTA GCGATGGACC GATCATCTCC 
AAGTGGGGTG CAATCTCCAG GTCATCCCGC ACCGGTTATC ACACGACAGA TCCTATTCAG 
GCCACTGCTT CCCAAGGAAG TGCTACTAAA CCCATCAGTG TATCAGATTA TGTCCCTTAT 
ATCAATGCTG TTGATTCAAG ATGGAGTGCC TATGGCTCAG ATTCTGCTTC ATCAGCACGT 
TATGCAGAAC GGGACAGGTT CATAGTTGCA GATTTGTCTG GTCACCGAAA GCATTCCAGC 
ACTGGAGATC TATTGAGTAT TGAATTACAG CAGGCCAAAA GTAACTCATT ATTACTTCAG 
AGAGAGGCAA ATGCACTAGC CATGCAACAG AAGTGGAATT CTCTAGATGA AGGCAGTCGT 
CTTACCTTAA ATCTTTTAAG CAAGGAAATT GATTTGAGGA ATGGTGAGGT AAAGAAAGGG 
ACTGATTATA CTGAAGACTG TGCAGACACG AAGCCAGATC GAGACATTGA ACTGGAGCTG 
TCAGCCCTGG ATACAGATGA ACCTGATGGG CAAGGTGAAC AAATAGAAGA GATTCTGGAT 
ATACAGCTAG GTATTAGTTC TCAAGATGAT CAGCTGCTCA ATGGGACAAC TGTAGAGAAT 
GGACATCTGC TAAAGCAGCA CCAGAAAGAA TCTATGGAAC AGAAGAGACA AAGTTTAGGT 
GAAGACCTTG TGATTCTGGA GGAGCAGAAA ACAATCCTGC CCGTAACTTC TTGCTTCAGT 
CAGCCGATCA CATCTGTTAG CAATGCAAGC TGCCTGCCCA TCAGCACAGC AGTCAGTGTT 
GGCAGCCTCA TTTTGAAAAC TGCTCACATT ATGTCTGAGG ATAAAAATGA CTTTTTAAAG 
CCTGTTGCAA GTGGCAGGAT GGTTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101867810 ( XM_031046428.1 ) cDNA ORF clone, Melopsittacus undulatus(budgerigar) -> XP_030902288.1 Melopsittacus undulatus ring finger and CCCH-type domains 2 (LOC101867810), mRNA.

Price & Availability↑

CloneID	OMe349033
Clone ID Related Accession (Same CDS sequence)	XM_031046428.1
Accession Version	XM_031046428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-03
Organism	Melopsittacus undulatus(budgerigar)
Product	LOW QUALITY PROTEIN: roquin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004848237.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Melopsittacus undulatus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTGTGC AGGCAGCTCA GTGGACAGAA TTTCTGTCCT GCCCAATCTG CTACAACGAG 
TTTGATGAGA ATGTGCACAA ACCCATCAGC TTAGGTTGCT CTCACACTGT CTGTAAGACC 
TGCCTGAACA AGCTTCATCG CAAGGCATGT CCTTTCGACC AGACTGCCAT CAACACGGAC 
ATCGATGTTC TTCCTGTAAA CTTCGCACTC CTCCAGCTAG TTGGAGCCCA GGTACCCGAT 
CATCAGACAG TAAAGTTGAG TAATGTAGGA GAGAACAAAC ATTATGAAGT AGCAAAGAAA 
TGTGTTGAGG ATTTGGCACT CTACTTAAAG CCATTAAGTG GAGGGAAAGG TGTTGCAAGC 
TTGAATCAGA GTGCACTGAG CCGTCCAATG CAAAGGAAGC TTGTGACACT GGTGAATTGT 
CAGCTGGTGG AGGAAGAGGG TCGGGTCAGA GCTATGAGAG CAGCTCGATC ACTGGGAGAG 
AGAACTGTCA CAGAACTGAT CTTGCAGCAC CAAAATCCTC AGCAGCTTTC TGCCAATCTT 
TGGGCTGCTG TCAGGGCACG AGGATGCCAG TTTCTAGGAC CAGCTATGCA AGAGGAGGCA 
CTGAAACTTG TATTACTGGC ACTGGAAGAC GGCTCTGCAC TCTCAAGAAA AGTTTTGGTA 
CTTTTTGTTG TGCAAAGGCT AGAACCAAGA TTTCCTCAGG CCTCTAAAAC AAGCATTGGT 
CATGTTGTGC AGCTACTGTA TAGAGCATCT TGCTTTAAGG TCACTAAAAG GGATGAAGAT 
TCTTCTCTGA TGCAGCTTAA AGAAGAGTTT CGGAGTTATG AGGCCTTGCG GAGAGAACAT 
GATGCCCAAA TTGTTCACAT TGCCATGGAA GCAGGACTTC GAATATCACC AGAACAATGG 
TCTTCTCTTC TGTATGGTGA CTTGGCACAT AAATCACACA TGCAATCCAT TATTGACAAG 
CCACAACCAA ATAGCAAATA CAAAACAAGT ATGTGCCGAG ACCTTCGACA GCAAGGGGGA 
TGTCCAAGAG GAACAAACTG TACATTTGCT CATTCTCAGG AAGAGCTCGA AAAATACCGC 
TTGAGGAACA AAAAAATCAG TGCAACAGTG AGAACATTCC CCCTTCTAAA TAAAGTTGGC 
GTAAATAGCA CCGTCTCAAC GACAACAGGA AACGTCATTT CTGTCATAGG AAGCCCTGAA 
GCAACAGGGA AGATGGTGCC AAGTACTAAC GGAATAGCTA ATCTGGAAAG TGGGGTTCCC 
CAGTTGATCC CTCGCTGTGC AGACACCTCC TTGAGAGCTT TGGAGAACAC CAAGAAGGGA 
GGGAAAACTG GAGCAAATGG CCAGAACGTT TCTGGATCCC CTACAGAATC ACTACCTGAA 
AATAAAATTG GTCTCCACCC AAGACTCCTG NNAAGCCAGG CAGCAGCTAC CTCAGCTGGT 
CCTCCTAATA TCGGAACAGA AGTTAATTCT GTGCCTCCAA AATCCAGCCC GTTTGTTCCC 
AGAGTACCTG TCTACCCTCC ACATTCTGAT AATGTTCAAT ATTTCCAAGA TCCCCGGACT 
CAGCTGTCAT ATGAAGTTCC ACAGTACCCA CAGACAGGAT ATTATCCACC ACCTCCAACA 
GTACCAGCTG GTGTGGCTCC CTGTGTTCCT CGCTTTGTGA GGTCCAATAA CGTTCCAGAG 
TCCTCCCTCC CACCTGCTTC CGTGCCATAT GCCGATCATT ACAGTACATT TCCCCCTCGA 
GATCGACTGA ATTCTCCTTA CCAACCTCCT CCTCCGCAGC CGTATGGACC AGTTCCTCCT 
GTCCCTTCTG GAATGTATGC TCCAGTTTAT GACAGCAGGC GCATCTGGCG CCCACAGATG 
TACCCACGAG ATGATATTAT TAGGAGCAAT TCTTTACCTC CCATGGATGT GATGCACTCA 
TCTGTCTATC AGACATCATT ACGTGAGAGA TACAACTCTT TGGATGGGTA TTACTCTGTG 
GCTTGTCAAC CTCCAAACGA GCAGAGGACT GTGCCTTTAC CAAGGGAGCC TTGTGGTCAT 
TTGAAGACTG GTTATGAAGA GCAGTTAAGA CGGAAGCCAG AGCAATGGGC ACAGTACCAC 
ACGCAGAAAA CTTCTCTGGT ATCGTCAACC CTTCCTATGG CAACACCATC TCCAACACCA 
CCTTCTCCTC TCTTCAGCGT AGATTTCAGC ACAGAGTTCT CAGAAAATGT CAGTGATTTG 
AGTGGAACTA AATTTGAGGA AGACCATCTC TCTCACTATT CACCGTGGTC TTGTGGCACT 
ATTGGCTCTT GTATAAATGC TATCGACTCA GAGCCCAAGG ATGTGATTGC CAATTCAAAT 
GCCGTGTTAA TGGATTTGGA CAGCGGGGAT GTTAAAAGGA GAGTGCATTT ATTTGAAACT 
CAGAGAAGGG CAAAGGAAGA AGATCCTATA ATCCCATTTA GCGATGGACC GATCATCTCC 
AAGTGGGGTG CAATCTCCAG GTCATCCCGC ACCGGTTATC ACACGACAGA TCCTATTCAG 
GCCACTGCTT CCCAAGGAAG TGCTACTAAA CCCATCAGTG TATCAGATTA TGTCCCTTAT 
ATCAATGCTG TTGATTCAAG ATGGAGTGCC TATGGCTCAG ATTCTGCTTC ATCAGCACGT 
TATGCAGAAC GGGACAGGTT CATAGTTGCA GATTTGTCTG GTCACCGAAA GCATTCCAGC 
ACTGGAGATC TATTGAGTAT TGAATTACAG CAGGCCAAAA GTAACTCATT ATTACTTCAG 
AGAGAGGCAA ATGCACTAGC CATGCAACAG AAGTGGAATT CTCTAGATGA AGGCAGTCGT 
CTTACCTTAA ATCTTTTAAG CAAGGAAATT GATTTGAGGA ATGGTGAGAC TGATTATACT 
GAAGACTGTG CAGACACGAA GCCAGATCGA GACATTGAAC TGGAGCTGTC AGCCCTGGAT 
ACAGATGAAC CTGATGGGCA AGGTGAACAA ATAGAAGAGA TTCTGGATAT ACAGCTAGGT 
ATTAGTTCTC AAGATGATCA GCTGCTCAAT GGGACAACTG TAGAGAATGG ACATCTGCTA 
AAGCAGCACC AGAAAGAATC TATGGAACAG AAGAGACAAA GTTTAGGTGA AGACCTTGTG 
ATTCTGGAGG AGCAGAAAAC AATCCTGCCC GTAACTTCTT GCTTCAGTCA GCCGATCACA 
TCTGTTAGCA ATGCAAGCTG CCTGCCCATC AGCACAGCAG TCAGTGTTGG CAGCCTCATT 
TTGAAAACTG CTCACATTAT GTCTGAGGAT AAAAATGACT TTTTAAAGCC TGTTGCAAGT 
GGCAGGATGG TTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030902288.1
CDS	35..3355
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQTVKLSNVGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKISATVRTFPLLNKVGVNSTVSTTTGNVISVIGSPEATGKMVPSTNGIANLESGVPQLIPRCADTSLRALENTKKGGKTGANGQNVSGSPTESLPENKIGLHPRLLnullSQAAATSAGPPNIGTEVNSVPPKSSPFVPRVPVYPPHSDNVQYFQDPRTQLSYEVPQYPQTGYYPPPPTVPAGVAPCVPRFVRSNNVPESSLPPASVPYADHYSTFPPRDRLNSPYQPPPPQPYGPVPPVPSGMYAPVYDSRRIWRPQMYPRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPNEQRTVPLPREPCGHLKTGYEEQLRRKPEQWAQYHTQKTSLVSSTLPMATPSPTPPSPLFSVDFSTEFSENVSDLSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFETQRRAKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPIQATASQGSATKPISVSDYVPYINAVDSRWSAYGSDSASSARYAERDRFIVADLSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGSRLTLNLLSKEIDLRNGETDYTEDCADTKPDRDIELELSALDTDEPDGQGEQIEEILDIQLGISSQDDQLLNGTTVENGHLLKQHQKESMEQKRQSLGEDLVILEEQKTILPVTSCFSQPITSVSNASCLPISTAVSVGSLILKTAHIMSEDKNDFLKPVASGRMVNS

Target ORF information:

RefSeq Version	XM_031046428.1
Organism	Melopsittacus undulatus(budgerigar)
Definition	Melopsittacus undulatus ring finger and CCCH-type domains 2 (LOC101867810), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031046428.1

ORF Insert Sequence:

ATGCCTGTGC AGGCAGCTCA GTGGACAGAA TTTCTGTCCT GCCCAATCTG CTACAACGAG 
TTTGATGAGA ATGTGCACAA ACCCATCAGC TTAGGTTGCT CTCACACTGT CTGTAAGACC 
TGCCTGAACA AGCTTCATCG CAAGGCATGT CCTTTCGACC AGACTGCCAT CAACACGGAC 
ATCGATGTTC TTCCTGTAAA CTTCGCACTC CTCCAGCTAG TTGGAGCCCA GGTACCCGAT 
CATCAGACAG TAAAGTTGAG TAATGTAGGA GAGAACAAAC ATTATGAAGT AGCAAAGAAA 
TGTGTTGAGG ATTTGGCACT CTACTTAAAG CCATTAAGTG GAGGGAAAGG TGTTGCAAGC 
TTGAATCAGA GTGCACTGAG CCGTCCAATG CAAAGGAAGC TTGTGACACT GGTGAATTGT 
CAGCTGGTGG AGGAAGAGGG TCGGGTCAGA GCTATGAGAG CAGCTCGATC ACTGGGAGAG 
AGAACTGTCA CAGAACTGAT CTTGCAGCAC CAAAATCCTC AGCAGCTTTC TGCCAATCTT 
TGGGCTGCTG TCAGGGCACG AGGATGCCAG TTTCTAGGAC CAGCTATGCA AGAGGAGGCA 
CTGAAACTTG TATTACTGGC ACTGGAAGAC GGCTCTGCAC TCTCAAGAAA AGTTTTGGTA 
CTTTTTGTTG TGCAAAGGCT AGAACCAAGA TTTCCTCAGG CCTCTAAAAC AAGCATTGGT 
CATGTTGTGC AGCTACTGTA TAGAGCATCT TGCTTTAAGG TCACTAAAAG GGATGAAGAT 
TCTTCTCTGA TGCAGCTTAA AGAAGAGTTT CGGAGTTATG AGGCCTTGCG GAGAGAACAT 
GATGCCCAAA TTGTTCACAT TGCCATGGAA GCAGGACTTC GAATATCACC AGAACAATGG 
TCTTCTCTTC TGTATGGTGA CTTGGCACAT AAATCACACA TGCAATCCAT TATTGACAAG 
CCACAACCAA ATAGCAAATA CAAAACAAGT ATGTGCCGAG ACCTTCGACA GCAAGGGGGA 
TGTCCAAGAG GAACAAACTG TACATTTGCT CATTCTCAGG AAGAGCTCGA AAAATACCGC 
TTGAGGAACA AAAAAATCAG TGCAACAGTG AGAACATTCC CCCTTCTAAA TAAAGTTGGC 
GTAAATAGCA CCGTCTCAAC GACAACAGGA AACGTCATTT CTGTCATAGG AAGCCCTGAA 
GCAACAGGGA AGATGGTGCC AAGTACTAAC GGAATAGCTA ATCTGGAAAG TGGGGTTCCC 
CAGTTGATCC CTCGCTGTGC AGACACCTCC TTGAGAGCTT TGGAGAACAC CAAGAAGGGA 
GGGAAAACTG GAGCAAATGG CCAGAACGTT TCTGGATCCC CTACAGAATC ACTACCTGAA 
AATAAAATTG GTCTCCACCC AAGACTCCTG NNAAGCCAGG CAGCAGCTAC CTCAGCTGGT 
CCTCCTAATA TCGGAACAGA AGTTAATTCT GTGCCTCCAA AATCCAGCCC GTTTGTTCCC 
AGAGTACCTG TCTACCCTCC ACATTCTGAT AATGTTCAAT ATTTCCAAGA TCCCCGGACT 
CAGCTGTCAT ATGAAGTTCC ACAGTACCCA CAGACAGGAT ATTATCCACC ACCTCCAACA 
GTACCAGCTG GTGTGGCTCC CTGTGTTCCT CGCTTTGTGA GGTCCAATAA CGTTCCAGAG 
TCCTCCCTCC CACCTGCTTC CGTGCCATAT GCCGATCATT ACAGTACATT TCCCCCTCGA 
GATCGACTGA ATTCTCCTTA CCAACCTCCT CCTCCGCAGC CGTATGGACC AGTTCCTCCT 
GTCCCTTCTG GAATGTATGC TCCAGTTTAT GACAGCAGGC GCATCTGGCG CCCACAGATG 
TACCCACGAG ATGATATTAT TAGGAGCAAT TCTTTACCTC CCATGGATGT GATGCACTCA 
TCTGTCTATC AGACATCATT ACGTGAGAGA TACAACTCTT TGGATGGGTA TTACTCTGTG 
GCTTGTCAAC CTCCAAACGA GCAGAGGACT GTGCCTTTAC CAAGGGAGCC TTGTGGTCAT 
TTGAAGACTG GTTATGAAGA GCAGTTAAGA CGGAAGCCAG AGCAATGGGC ACAGTACCAC 
ACGCAGAAAA CTTCTCTGGT ATCGTCAACC CTTCCTATGG CAACACCATC TCCAACACCA 
CCTTCTCCTC TCTTCAGCGT AGATTTCAGC ACAGAGTTCT CAGAAAATGT CAGTGATTTG 
AGTGGAACTA AATTTGAGGA AGACCATCTC TCTCACTATT CACCGTGGTC TTGTGGCACT 
ATTGGCTCTT GTATAAATGC TATCGACTCA GAGCCCAAGG ATGTGATTGC CAATTCAAAT 
GCCGTGTTAA TGGATTTGGA CAGCGGGGAT GTTAAAAGGA GAGTGCATTT ATTTGAAACT 
CAGAGAAGGG CAAAGGAAGA AGATCCTATA ATCCCATTTA GCGATGGACC GATCATCTCC 
AAGTGGGGTG CAATCTCCAG GTCATCCCGC ACCGGTTATC ACACGACAGA TCCTATTCAG 
GCCACTGCTT CCCAAGGAAG TGCTACTAAA CCCATCAGTG TATCAGATTA TGTCCCTTAT 
ATCAATGCTG TTGATTCAAG ATGGAGTGCC TATGGCTCAG ATTCTGCTTC ATCAGCACGT 
TATGCAGAAC GGGACAGGTT CATAGTTGCA GATTTGTCTG GTCACCGAAA GCATTCCAGC 
ACTGGAGATC TATTGAGTAT TGAATTACAG CAGGCCAAAA GTAACTCATT ATTACTTCAG 
AGAGAGGCAA ATGCACTAGC CATGCAACAG AAGTGGAATT CTCTAGATGA AGGCAGTCGT 
CTTACCTTAA ATCTTTTAAG CAAGGAAATT GATTTGAGGA ATGGTGAGAC TGATTATACT 
GAAGACTGTG CAGACACGAA GCCAGATCGA GACATTGAAC TGGAGCTGTC AGCCCTGGAT 
ACAGATGAAC CTGATGGGCA AGGTGAACAA ATAGAAGAGA TTCTGGATAT ACAGCTAGGT 
ATTAGTTCTC AAGATGATCA GCTGCTCAAT GGGACAACTG TAGAGAATGG ACATCTGCTA 
AAGCAGCACC AGAAAGAATC TATGGAACAG AAGAGACAAA GTTTAGGTGA AGACCTTGTG 
ATTCTGGAGG AGCAGAAAAC AATCCTGCCC GTAACTTCTT GCTTCAGTCA GCCGATCACA 
TCTGTTAGCA ATGCAAGCTG CCTGCCCATC AGCACAGCAG TCAGTGTTGG CAGCCTCATT 
TTGAAAACTG CTCACATTAT GTCTGAGGAT AAAAATGACT TTTTAAAGCC TGTTGCAAGT 
GGCAGGATGG TTAACAGCTG A