LOC105663136 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105663136
Entrez Gene ID	105663136
Full Name	cytochrome P450 9e2-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012290381.1	XP_012145771.1	cytochrome P450 9e2-like

Pathways from BioSystems
Gene Ontology

The following LOC105663136 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105663136 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe109072	XM_012290381.1 Latest version!	Megachile rotundata cytochrome P450 9e2-like (LOC105663136), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105663136 ( XM_012290381.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145771.1 Megachile rotundata cytochrome P450 9e2-like (LOC105663136), mRNA.

Price & Availability↑

CloneID	OMe109072
Clone ID Related Accession (Same CDS sequence)	XM_012290381.1
Accession Version	XM_012290381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	cytochrome P450 9e2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCG CGTCGTTTTC TTTTTCACTG AAGCTGTTGG TGATCGCTTT TTCCATCGTG 
GGTTTCGCCA AGCTCATCTC GATCGTGTAC ATTCAGCACA CCTACTGGAA AAAGAAGCAC 
GTTCCCTACA TCAAAGGGAT GCCGGTGTTC GGCTCCTCCT GGAAGCTTTT TCTCCGCCGC 
ATTTCCTTTC CAGACTGTTG CAAATTCATC TACAATTATT ACCCCGACCT ACGGTACGTG 
TGCGTGATGG ATTTCTCGAC GCCGGCGGTG GTGATCCGCG ACCCGGAGCT GATCCGGGAG 
ATCGCCATCA AGAATTTCGA GTACTTCCCC GATCACCAGA GCTTCATCAA CGAAAAGATC 
GACCCGATTT TCGGGAAGAA CGTGTTCTCC CTGCGGGGGG AGCGCTGGAA GGAGATGAGG 
AGCACTTTGA GCCCATCTTT CACCGCCAGC AAGATGCGCT TCCTCTTCGA GTTGGTGTCC 
AAGTGCGCCC AGGAGTTCGT CAACTATTTA TACAACCATC CGGAGTTCTC CGCCTCCATA 
GAGGTGAAGG ACGCGTTCAC CAGGTACACG AACGACACCA TCGCCACGGT GGCTTTCGGC 
ATCAGCGTGA ACTCGCTGAT GGACCGCGAG AACGAGTTCT ACAAGAAAGG AGCCGACGCC 
ACGAACTTCG CTGGCCTCTT CCGTCTGATC AAGTTCCTGC TCTTCCGTAT GAACCCTCGT 
TTAACAAGGA TGGCCGGGTT CTCGTTCCTG TCGCGAGACA CCGCCAGCTT CTTCAAGAAA 
GTCGTCTTCG AGACTGTGAA GGCGCGGGAC GAGCAGAATA TCGTCCGGCC CGACATGATT 
CATCTTCTGA TGGAGGCCAG GGATAAAGAG AAGCCCGTGT CGCGTCAGAT GACCATCGAT 
GATATCACCG CTCAGGCGTT CATCTTCTTC TTGGCCGGTT TCGATACCTC CTCCAAGCTG 
ATGTGCTACA TGGTTCACGA GCTGGCCGTT AATCCGGACA TTCAGGAGAA GCTGCGCGAC 
GAGGTGGATC GTTACATCCG CGAGGGGGAC GGCGATATAT CGTACGACGC GCTGTCCAGG 
ATGAAGTACA TGGAGATGGT GACTGCGGAG GCACTCAGGA AGTATCCGCC GCTCGTCCAC 
ATCGATAGAC TCTGCGCACG GAAGTTTCAG CTACCTCCGG CTGGACCTGG ATACGATAGC 
GTCACGATAT ATCCGGACAA TGTTGTTCTG TTCCCCGTTT ACGCGCTGCA TCACGACCCT 
AAATACTTTC CAGACCCGGA CAAGTTCGAC CCTGAGCGTT TCAGTGAGGA GAATAAAGAT 
AAGATCGACC CTTACACGTA TCTGCCCTTC GGGCTGGGAC CCAGGAAGTG TATCGGCAGT 
CGCTTCGCGT TGATGGAGAC CAAGATCTTG ATCGCTCATC TTTTGCATCG GTTCTACTTG 
AAGTGCACCG AAAAGACTAA GGTACAGATC GAGTTTAGCA AGAAGAACTT CTCGATAACG 
CCTGATGGCG GGTTTTGGAT AGGTCTGGAG AGTAGGAGTT CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145771.1
CDS	36..1583
Translation	MESASFSFSLKLLVIAFSIVGFAKLISIVYIQHTYWKKKHVPYIKGMPVFGSSWKLFLRRISFPDCCKFIYNYYPDLRYVCVMDFSTPAVVIRDPELIREIAIKNFEYFPDHQSFINEKIDPIFGKNVFSLRGERWKEMRSTLSPSFTASKMRFLFELVSKCAQEFVNYLYNHPEFSASIEVKDAFTRYTNDTIATVAFGISVNSLMDRENEFYKKGADATNFAGLFRLIKFLLFRMNPRLTRMAGFSFLSRDTASFFKKVVFETVKARDEQNIVRPDMIHLLMEARDKEKPVSRQMTIDDITAQAFIFFLAGFDTSSKLMCYMVHELAVNPDIQEKLRDEVDRYIREGDGDISYDALSRMKYMEMVTAEALRKYPPLVHIDRLCARKFQLPPAGPGYDSVTIYPDNVVLFPVYALHHDPKYFPDPDKFDPERFSEENKDKIDPYTYLPFGLGPRKCIGSRFALMETKILIAHLLHRFYLKCTEKTKVQIEFSKKNFSITPDGGFWIGLESRSSV

Target ORF information:

RefSeq Version	XM_012290381.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata cytochrome P450 9e2-like (LOC105663136), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290381.1

ORF Insert Sequence:

ATGGAGTCCG CGTCGTTTTC TTTTTCACTG AAGCTGTTGG TGATCGCTTT TTCCATCGTG 
GGTTTCGCCA AGCTCATCTC GATCGTGTAC ATTCAGCACA CCTACTGGAA AAAGAAGCAC 
GTTCCCTACA TCAAAGGGAT GCCGGTGTTC GGCTCCTCCT GGAAGCTTTT TCTCCGCCGC 
ATTTCCTTTC CAGACTGTTG CAAATTCATC TACAATTATT ACCCCGACCT ACGGTACGTG 
TGCGTGATGG ATTTCTCGAC GCCGGCGGTG GTGATCCGCG ACCCGGAGCT GATCCGGGAG 
ATCGCCATCA AGAATTTCGA GTACTTCCCC GATCACCAGA GCTTCATCAA CGAAAAGATC 
GACCCGATTT TCGGGAAGAA CGTGTTCTCC CTGCGGGGGG AGCGCTGGAA GGAGATGAGG 
AGCACTTTGA GCCCATCTTT CACCGCCAGC AAGATGCGCT TCCTCTTCGA GTTGGTGTCC 
AAGTGCGCCC AGGAGTTCGT CAACTATTTA TACAACCATC CGGAGTTCTC CGCCTCCATA 
GAGGTGAAGG ACGCGTTCAC CAGGTACACG AACGACACCA TCGCCACGGT GGCTTTCGGC 
ATCAGCGTGA ACTCGCTGAT GGACCGCGAG AACGAGTTCT ACAAGAAAGG AGCCGACGCC 
ACGAACTTCG CTGGCCTCTT CCGTCTGATC AAGTTCCTGC TCTTCCGTAT GAACCCTCGT 
TTAACAAGGA TGGCCGGGTT CTCGTTCCTG TCGCGAGACA CCGCCAGCTT CTTCAAGAAA 
GTCGTCTTCG AGACTGTGAA GGCGCGGGAC GAGCAGAATA TCGTCCGGCC CGACATGATT 
CATCTTCTGA TGGAGGCCAG GGATAAAGAG AAGCCCGTGT CGCGTCAGAT GACCATCGAT 
GATATCACCG CTCAGGCGTT CATCTTCTTC TTGGCCGGTT TCGATACCTC CTCCAAGCTG 
ATGTGCTACA TGGTTCACGA GCTGGCCGTT AATCCGGACA TTCAGGAGAA GCTGCGCGAC 
GAGGTGGATC GTTACATCCG CGAGGGGGAC GGCGATATAT CGTACGACGC GCTGTCCAGG 
ATGAAGTACA TGGAGATGGT GACTGCGGAG GCACTCAGGA AGTATCCGCC GCTCGTCCAC 
ATCGATAGAC TCTGCGCACG GAAGTTTCAG CTACCTCCGG CTGGACCTGG ATACGATAGC 
GTCACGATAT ATCCGGACAA TGTTGTTCTG TTCCCCGTTT ACGCGCTGCA TCACGACCCT 
AAATACTTTC CAGACCCGGA CAAGTTCGAC CCTGAGCGTT TCAGTGAGGA GAATAAAGAT 
AAGATCGACC CTTACACGTA TCTGCCCTTC GGGCTGGGAC CCAGGAAGTG TATCGGCAGT 
CGCTTCGCGT TGATGGAGAC CAAGATCTTG ATCGCTCATC TTTTGCATCG GTTCTACTTG 
AAGTGCACCG AAAAGACTAA GGTACAGATC GAGTTTAGCA AGAAGAACTT CTCGATAACG 
CCTGATGGCG GGTTTTGGAT AGGTCTGGAG AGTAGGAGTT CTGTTTGA