LOC105662578 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105662578
Entrez Gene ID	105662578
Full Name	yrdC domain-containing protein, mitochondrial
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012286174.1	XP_012141564.1	yrdC domain-containing protein, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC105662578 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105662578 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe105420	XM_012286174.1 Latest version!	Megachile rotundata yrdC domain-containing protein, mitochondrial (LOC105662578), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105662578 ( XM_012286174.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141564.1 Megachile rotundata yrdC domain-containing protein, mitochondrial (LOC105662578), mRNA.

Price & Availability↑

CloneID	OMe105420
Clone ID Related Accession (Same CDS sequence)	XM_012286174.1
Accession Version	XM_012286174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	yrdC domain-containing protein, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797177.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGAAGACTC TTTTAAATTC TGCCTCGATG GATCTCAAGA CATTGAGAAA GTCCAATAAA 
CATTGGAACT GTAGGGGAAG AAGGAGCGTG GCTATAGCTG CTACGCTATT ACAGCAAAAT 
TCAATTGTAG CAATTCCAAC GGATACAGTG TATGGTCTAG CTGGTGTCGT GTCCAATAAC 
GCGACTATTC AAATGCTATA CAACATTAAG AGTCGCGATA CTAGCAAACC TTTATCTATT 
TCTGTTAGCA GCGTAAGCGA CATAAAAATG TGGGGTATTA CGGATCATTT ACCAGCAGAT 
TTATTGCCAA CAATTTTACC TGGTCCATAT ACAGTTATCT TAAAGCGGAC TCCGGCGTTA 
AATCCTGCTT TGAATCCAGA TATCGATACA GTAGGCATAA GAGTTCCAAA ATTTCACTTT 
ATAAACTGCG TTTCAAGATT AACAGGACCT TTGGCGCTAA CAAGCGCCAA CGAAAGCAAC 
GAACCAAGTT GTCTATATGC TTCGGAATTC GAGAAGTTAT GGCCTTTGTT AGGTGGAATA 
TTTTACGATG GTACTATTCA TGGCCGTTCG AATGAACGTT TACGGAAAGG ATCAACGATA 
GTTGATTTAT CGCAACCAGG TTGTTACAAA ATCGTACGAC GAGGGGTTGG AGCTCTGTCA 
CTTTGTATTA CCTTAAAACA TTTCGGGTTA GAAAAAGACG AATCTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141564.1
CDS	1..711
Translation	MKTLLNSASMDLKTLRKSNKHWNCRGRRSVAIAATLLQQNSIVAIPTDTVYGLAGVVSNNATIQMLYNIKSRDTSKPLSISVSSVSDIKMWGITDHLPADLLPTILPGPYTVILKRTPALNPALNPDIDTVGIRVPKFHFINCVSRLTGPLALTSANESNEPSCLYASEFEKLWPLLGGIFYDGTIHGRSNERLRKGSTIVDLSQPGCYKIVRRGVGALSLCITLKHFGLEKDESN

Target ORF information:

RefSeq Version	XM_012286174.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata yrdC domain-containing protein, mitochondrial (LOC105662578), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286174.1

ORF Insert Sequence:

ATGAAGACTC TTTTAAATTC TGCCTCGATG GATCTCAAGA CATTGAGAAA GTCCAATAAA 
CATTGGAACT GTAGGGGAAG AAGGAGCGTG GCTATAGCTG CTACGCTATT ACAGCAAAAT 
TCAATTGTAG CAATTCCAAC GGATACAGTG TATGGTCTAG CTGGTGTCGT GTCCAATAAC 
GCGACTATTC AAATGCTATA CAACATTAAG AGTCGCGATA CTAGCAAACC TTTATCTATT 
TCTGTTAGCA GCGTAAGCGA CATAAAAATG TGGGGTATTA CGGATCATTT ACCAGCAGAT 
TTATTGCCAA CAATTTTACC TGGTCCATAT ACAGTTATCT TAAAGCGGAC TCCGGCGTTA 
AATCCTGCTT TGAATCCAGA TATCGATACA GTAGGCATAA GAGTTCCAAA ATTTCACTTT 
ATAAACTGCG TTTCAAGATT AACAGGACCT TTGGCGCTAA CAAGCGCCAA CGAAAGCAAC 
GAACCAAGTT GTCTATATGC TTCGGAATTC GAGAAGTTAT GGCCTTTGTT AGGTGGAATA 
TTTTACGATG GTACTATTCA TGGCCGTTCG AATGAACGTT TACGGAAAGG ATCAACGATA 
GTTGATTTAT CGCAACCAGG TTGTTACAAA ATCGTACGAC GAGGGGTTGG AGCTCTGTCA 
CTTTGTATTA CCTTAAAACA TTTCGGGTTA GAAAAAGACG AATCTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.