LOC100883379 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883379
Entrez Gene ID	100883379
Full Name	dystrobrevin beta
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003707170.2	XP_003707218.1	dystrobrevin beta

Pathways from BioSystems
Gene Ontology

The following LOC100883379 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883379 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe112606	XM_003707170.2 Latest version!	Megachile rotundata dystrobrevin beta (LOC100883379), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883379 ( XM_003707170.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003707218.1 Megachile rotundata dystrobrevin beta (LOC100883379), mRNA.

Price & Availability↑

CloneID	OMe112606
Clone ID Related Accession (Same CDS sequence)	XM_003707170.2
Accession Version	XM_003707170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	dystrobrevin beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797515.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG ATGGCGCAGG AGGATCAGGA AATGGTAGCA CAGGAGAGGC AAGTCGATTG 
CAGCTCCTGC AAGAAATGCG ACAGCAAAAT TTCGACAGCA TTCGATTTGC ATCCTATCGC 
ACAGCTTGCA AGCTGAGATT TATTCAAAAG AAAGTTCATC TACATAACGT CGACATATGG 
AATGTAATCG AGGCGTTTCG AGAAAACGGT TTGAACACAC TGGAACCATC GAGCACATTG 
GGAGTCTCGA GGCTCGAAAC TTTATTGTCT TCCTTATTTC ACGCTCTTAA TAAACGAGTG 
CCTGTTTCGC AACAAGCCAA AGTCGATGCT ACAACCGCCC TGTTGATGAA CTGGTTACTT 
GCCGCCTACA CTACAGGGGA AAACAATAAA ATATCCGTAT TCTCGGTAAA AGTAGCTTTG 
GCTACATTAT GTGCAGGGAA GCTGATGGAC AAATTTCGAT ATATATATTC CCAAATATCC 
GATAGTAACG GTCACATGAT ACATTGGAGA TTCGCCGAAT ATTTAAAAGA AGTTTTAGCG 
TTAACGGCCG CAGTTTACGA ATCACCATCT TTCGGCTACT CCGAGGGTCT CGCCAATTCA 
ATATTTCCTC AAAACTCGAA AGTCACGGTT AACGATTTTT TGGATACGCT TATGTCTGAT 
CCAGGACCAC ACTGTTTGAT CTGGCTTCCA CTGTACCATA GAATGGCTGC TGTTGAAACA 
GTGGCCCATC CTGTTATGTG TGATGCGTGT CACAAAGAGA ACTTCACTGG CTTCCGATAT 
AGATGTCAAA AATGTCACTC GTATCAGCTT TGTCAGGACT GTTTTTGGCG TGGTAAAGTT 
TCTGGGACAC ACAATAACGA TCACGAGACG AGGGAGTACA GTAGTTTCAA ATCACCTAGC 
AAACAAATTG GTCATTCCTT GAGGAAGAGC TTTAGATGTG TACCTGAAAA GGGGAAGAAC 
AGTTTACCAC GATTTCCGGA ACAGCCGGAG AAAACGTTAG ATTTATCGCA CATAGTCCCA 
CCTTCACCTT TACCATCTCA CAACGGATTT CCAGATCCAG GTTTTATGGC TCCCTTCGAT 
TCTGGATCGA TGGATAGCCG TTCTACCTTG AGGAGTATGG ATAGTTCAAG ACTGGATGAT 
GAACATAAAT TAATAGCACG ATACGCACAA AGGTTGGCTC AGGAAGCTAG GACTATGCCC 
CGCGCCGCAT CTAGAATGTC GCAAGCTGAT CAAGCAGGTA GGGCTCCGTC TGATGCAAAT 
TTGGCATCAT TAGATGCTTC AAGAGCGCAA CGTGAACTTA TATCGCAGTT AGAAGCAAAG 
AACAAGGAGA TAATGCGCGA GATTGCAAGG TTAAGGAGAC AGCAAGAAAT AGAAGCAGCT 
GGTTTAGAAA ATCCTGCACT AATGTCTGAG TTGAGAGCCT TAAGGCAGCG AAAAGATGAA 
TTAGAAACAC ACTTAGCAAC ATTACAAGAC TCCAGAAGAC AATTAATGGT GCAGCTAGAA 
GGTTTAATGA AAATGTTAAA GAATCATCAG GCATCCCCAA GATCAACACC AAATAGTTCA 
CCACGAAGTA CAAAATCACC ACCTTTACCA CCTGGTGCTG TACCCAGTAG TAGATCAGCA 
CCTCCAACTC CAGGTGGTCC ACTTTCTACG ACACCTCAAC AACAGCAACA ACAATCACAA 
AGCCAACAAC AAATGTCTCA AAGTTATCAG AGTTCTGTAC CAACGACCTC GGTGGCGAGT 
GTAACCAATA ATACCATGCT GGGAACAATA CAAAATCCTA TTCCAGATAA TTTATCGTGT 
GTTGGAGGTG ATGTAAGGTC TGCGTTCAGG ACAAACAGCT TGCCAGGAAG TGGTTCCAGC 
AGTGCGAATA ATAGCTTGGG TCGATCACTA AGAAATGACT TGTTGGTAGC TGCTGACAGT 
GTTACTAATG CCATGTCGAC ACTCGTGAGG GAATTAAATT CGGACGTGTT GTGGAATGTG 
TGTGATGCAC CCCTTGTGAA TGTTTCCTGG TGCCTTGCAG ACTCAGACCA GGAGGATCAT 
TCTCACAACT CTGGACGAAT TAACATCAGA AAACCGTTAG ATTTCGAGGC TGGTGAGGAT 
GGAGATGATA GTAGTGGTGG TGGTAATTCA TGGAGAGAAG AACTTCAGCG TCGTTATCAA 
CAAGAAAATG ATTTCTTGGC TGAATTACGT GCACGCAATG TTAACATGTC ACAAACACCA 
TCTGCTACAC CATCCAGCGA ACAAGAACAG GAAAGGGGAG AAAGAGAAGA AGCAGATGGA 
GAAAAGGAGG ATGAAAATGA ATCTAACTGG ACAGAACCAG TGAAAAGATG GGTTAATCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003707218.1
CDS	445..2787
Translation	MAEDGAGGSGNGSTGEASRLQLLQEMRQQNFDSIRFASYRTACKLRFIQKKVHLHNVDIWNVIEAFRENGLNTLEPSSTLGVSRLETLLSSLFHALNKRVPVSQQAKVDATTALLMNWLLAAYTTGENNKISVFSVKVALATLCAGKLMDKFRYIYSQISDSNGHMIHWRFAEYLKEVLALTAAVYESPSFGYSEGLANSIFPQNSKVTVNDFLDTLMSDPGPHCLIWLPLYHRMAAVETVAHPVMCDACHKENFTGFRYRCQKCHSYQLCQDCFWRGKVSGTHNNDHETREYSSFKSPSKQIGHSLRKSFRCVPEKGKNSLPRFPEQPEKTLDLSHIVPPSPLPSHNGFPDPGFMAPFDSGSMDSRSTLRSMDSSRLDDEHKLIARYAQRLAQEARTMPRAASRMSQADQAGRAPSDANLASLDASRAQRELISQLEAKNKEIMREIARLRRQQEIEAAGLENPALMSELRALRQRKDELETHLATLQDSRRQLMVQLEGLMKMLKNHQASPRSTPNSSPRSTKSPPLPPGAVPSSRSAPPTPGGPLSTTPQQQQQQSQSQQQMSQSYQSSVPTTSVASVTNNTMLGTIQNPIPDNLSCVGGDVRSAFRTNSLPGSGSSSANNSLGRSLRNDLLVAADSVTNAMSTLVRELNSDVLWNVCDAPLVNVSWCLADSDQEDHSHNSGRINIRKPLDFEAGEDGDDSSGGGNSWREELQRRYQQENDFLAELRARNVNMSQTPSATPSSEQEQERGEREEADGEKEDENESNWTEPVKRWVNR

Target ORF information:

RefSeq Version	XM_003707170.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata dystrobrevin beta (LOC100883379), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707170.2

ORF Insert Sequence:

ATGGCGGAGG ATGGCGCAGG AGGATCAGGA AATGGTAGCA CAGGAGAGGC AAGTCGATTG 
CAGCTCCTGC AAGAAATGCG ACAGCAAAAT TTCGACAGCA TTCGATTTGC ATCCTATCGC 
ACAGCTTGCA AGCTGAGATT TATTCAAAAG AAAGTTCATC TACATAACGT CGACATATGG 
AATGTAATCG AGGCGTTTCG AGAAAACGGT TTGAACACAC TGGAACCATC GAGCACATTG 
GGAGTCTCGA GGCTCGAAAC TTTATTGTCT TCCTTATTTC ACGCTCTTAA TAAACGAGTG 
CCTGTTTCGC AACAAGCCAA AGTCGATGCT ACAACCGCCC TGTTGATGAA CTGGTTACTT 
GCCGCCTACA CTACAGGGGA AAACAATAAA ATATCCGTAT TCTCGGTAAA AGTAGCTTTG 
GCTACATTAT GTGCAGGGAA GCTGATGGAC AAATTTCGAT ATATATATTC CCAAATATCC 
GATAGTAACG GTCACATGAT ACATTGGAGA TTCGCCGAAT ATTTAAAAGA AGTTTTAGCG 
TTAACGGCCG CAGTTTACGA ATCACCATCT TTCGGCTACT CCGAGGGTCT CGCCAATTCA 
ATATTTCCTC AAAACTCGAA AGTCACGGTT AACGATTTTT TGGATACGCT TATGTCTGAT 
CCAGGACCAC ACTGTTTGAT CTGGCTTCCA CTGTACCATA GAATGGCTGC TGTTGAAACA 
GTGGCCCATC CTGTTATGTG TGATGCGTGT CACAAAGAGA ACTTCACTGG CTTCCGATAT 
AGATGTCAAA AATGTCACTC GTATCAGCTT TGTCAGGACT GTTTTTGGCG TGGTAAAGTT 
TCTGGGACAC ACAATAACGA TCACGAGACG AGGGAGTACA GTAGTTTCAA ATCACCTAGC 
AAACAAATTG GTCATTCCTT GAGGAAGAGC TTTAGATGTG TACCTGAAAA GGGGAAGAAC 
AGTTTACCAC GATTTCCGGA ACAGCCGGAG AAAACGTTAG ATTTATCGCA CATAGTCCCA 
CCTTCACCTT TACCATCTCA CAACGGATTT CCAGATCCAG GTTTTATGGC TCCCTTCGAT 
TCTGGATCGA TGGATAGCCG TTCTACCTTG AGGAGTATGG ATAGTTCAAG ACTGGATGAT 
GAACATAAAT TAATAGCACG ATACGCACAA AGGTTGGCTC AGGAAGCTAG GACTATGCCC 
CGCGCCGCAT CTAGAATGTC GCAAGCTGAT CAAGCAGGTA GGGCTCCGTC TGATGCAAAT 
TTGGCATCAT TAGATGCTTC AAGAGCGCAA CGTGAACTTA TATCGCAGTT AGAAGCAAAG 
AACAAGGAGA TAATGCGCGA GATTGCAAGG TTAAGGAGAC AGCAAGAAAT AGAAGCAGCT 
GGTTTAGAAA ATCCTGCACT AATGTCTGAG TTGAGAGCCT TAAGGCAGCG AAAAGATGAA 
TTAGAAACAC ACTTAGCAAC ATTACAAGAC TCCAGAAGAC AATTAATGGT GCAGCTAGAA 
GGTTTAATGA AAATGTTAAA GAATCATCAG GCATCCCCAA GATCAACACC AAATAGTTCA 
CCACGAAGTA CAAAATCACC ACCTTTACCA CCTGGTGCTG TACCCAGTAG TAGATCAGCA 
CCTCCAACTC CAGGTGGTCC ACTTTCTACG ACACCTCAAC AACAGCAACA ACAATCACAA 
AGCCAACAAC AAATGTCTCA AAGTTATCAG AGTTCTGTAC CAACGACCTC GGTGGCGAGT 
GTAACCAATA ATACCATGCT GGGAACAATA CAAAATCCTA TTCCAGATAA TTTATCGTGT 
GTTGGAGGTG ATGTAAGGTC TGCGTTCAGG ACAAACAGCT TGCCAGGAAG TGGTTCCAGC 
AGTGCGAATA ATAGCTTGGG TCGATCACTA AGAAATGACT TGTTGGTAGC TGCTGACAGT 
GTTACTAATG CCATGTCGAC ACTCGTGAGG GAATTAAATT CGGACGTGTT GTGGAATGTG 
TGTGATGCAC CCCTTGTGAA TGTTTCCTGG TGCCTTGCAG ACTCAGACCA GGAGGATCAT 
TCTCACAACT CTGGACGAAT TAACATCAGA AAACCGTTAG ATTTCGAGGC TGGTGAGGAT 
GGAGATGATA GTAGTGGTGG TGGTAATTCA TGGAGAGAAG AACTTCAGCG TCGTTATCAA 
CAAGAAAATG ATTTCTTGGC TGAATTACGT GCACGCAATG TTAACATGTC ACAAACACCA 
TCTGCTACAC CATCCAGCGA ACAAGAACAG GAAAGGGGAG AAAGAGAAGA AGCAGATGGA 
GAAAAGGAGG ATGAAAATGA ATCTAACTGG ACAGAACCAG TGAAAAGATG GGTTAATCGA 
TAA