LOC100882830 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100882830
Entrez Gene ID	100882830
Full Name	male-specific lethal 1 homolog
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003700437.2	XP_003700485.1	male-specific lethal 1 homolog

Pathways from BioSystems
Gene Ontology

The following LOC100882830 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100882830 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe98850	XM_003700437.2 Latest version!	Megachile rotundata male-specific lethal 1 homolog (LOC100882830), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100882830 ( XM_003700437.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003700485.1 Megachile rotundata male-specific lethal 1 homolog (LOC100882830), mRNA.

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CloneID	OMe98850
Clone ID Related Accession (Same CDS sequence)	XM_003700437.2
Accession Version	XM_003700437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	male-specific lethal 1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003700437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGTG GCTCGGCCTC GAAACTCGTG AACGGTGAAA ACAAGAAGGA GAAGGTTCAC 
GGGTACGGGT ACGGGTACGG TTACGGGTAC GGGTACGGGT ACGAATACGA GTACGAGTAC 
GAGAACAGGA AAGAAACCGA GAGCGGCGCT GCTGGCAGCA CTGCCTCTTC CATAGTTCTC 
GCTGGTGGTA CTAGTAACGC TACTACTATC ATTACTGTTG CTGGTACTGG TACTACTGTT 
CCTAGTATTA CTGGTAACGG CGGTAGTGGT AGTGGTAGTG GTTGTGGTAA CGGTGGTAGT 
GTTCGTTTCA CCGATAGTGG TAACGGTGAC AGTGATAGTA ATAGCGGTGT TTGCGTTACC 
GGCTTGACCG TCGGGGGCGT CGTCGGCGTG GAGTTTCGCG ACGTTAATTC CGATCTGAAA 
GTACCAGAGA ATTTCGAAGA GGTAGACAGT CAGAGGGATG TAAACGAGAG GACGGAATTC 
GGTGAAACCG AGTTTCAGGA CCCGTACACG GGAACGTGTA TCGAGACGCC AGTCGTCGAT 
CTCGCTACTA CTACTACCAC CACCACCGCC ACAACCACCA CCACCATCAC CATCATCGAA 
CACAACGATC GAACGAATAC GACCCACGGT GTTTCTGCTC CTTGTTACGA AGAAGATCAG 
GAAGAGGATA ACGAGGAGGA CGACGACAAC ATGGTCGCGG CGATGAACGA ACACTTGTCT 
ACCACGTCGG CGACCAGCGG CATTCGAAGT AGCACTGTTT CGACAACCAG TACCACTGGT 
AATTCCGTCG TCAACCAAAA TAACAGTTGC TCTCGTAATG AATTTCAAAA CGTTGAGGAG 
GATAGTAAAC CGGCCGCTGG AATACTCAGT TTTCCCTGTG ATTTCGATCA CATGTATGCT 
AGCATGACAG ACGGCGGAGC TCCGGCAGCA GCCACTGCCA CTTCTGCTCT GGGTGTCAGT 
CCTCTTCGAG GTAATGAACC GCGTCAAAAC GATCTTACCG AGGTTAAACA CCTCAAGGAA 
CTTTTGCTTC TTCATTTGGA TTTAATTCAA CAACAATCCG AACAAATTGT CACCAAGGAT 
AAACTCTTAG CTGCTCTACG ACAGGAAAAC GAAACGCTTA AGTTGCGTTT GGAGCGTATG 
GATAGACGTG TAAATTTACA AAAGTTACGA TCAGAGAACA GCGAAAACTC CATTACACCA 
GAACATATAG GAGCATGTTC TCCTCCAACT GTTAATTCTG TGAATGTGCC ATCGACTAGT 
GAACTTCACA GGAATAATCA GTCTGAAAAT AATAATTCCC AATACAATGA AAGTTTTAAA 
ATACGTTTAA ATACTAGCGG CGGAATTACT ACGGTTAAAC AAGAACCAAA CGAGAATCAA 
AGTAAGCACG AGACTAAAAT AGAAACTGGC AATGATGCGA GGTTAGATTG GGAAGACAAA 
AAGAAAAGAA GAACAGATCC GATACCATTG TCCACTGGAA GTAAAAGAAA AAGAGGTGTT 
TCGTGTTCGT CTACGATTAG TAATGATACA TCGTCAACGG TACACGATAA GACGTTAACT 
CAGGAGAGCG GTAGGAAAAG TGATAGAAAA GGAAAGAGTA TAACAAAAAA GGATTCTTTT 
CTTACAACAG AGGAACATTA TTACACGGCT GTTGGTGATC CGAATTATAC CTTAAGTTTA 
AAAGAACCTA ATCCTGTGGA AAGTGATACT TCGTTAGAAG TTCCAAATTG GAGGGTAAAG 
ATATATACAA GTTGTTATAC AATGGAAGGA ACTGAAAATT TGGACGACGA AATTTTTAAC 
AAAAGACATT TAAAATTAGA AAATGACGAA AGAAGGAGAA AAAGATGGGA TGTACAAAGG 
ATACGAGAAC AGAGGCACAT CGAAAAGTTG AAACAAAGAC AGGAACGGCA GAATCATCAA 
GCTACATGTT ATAATACAAA TCATACAGGA CCTTGTCCAT CAACGGTCGA GGAAGAGTCT 
ATATCATCCT TGTGGCCTGA TGTAGAACAG ATACAAAGTC TTCAAGTGGA TCCACAATTA 
CCTGTTACTG CATTTGGTGC TCCTATTCCT AGTTTTACTC CAAGTGAATT TTCTTTACCA 
TGGTTAAATA TGTCACGATC GAGTTGCCGG CGTCCAAAGC GATCAACAGG TAGAAGAAAA 
CCTACTAGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003700485.1
CDS	356..2530
Translation	MSSGSASKLVNGENKKEKVHGYGYGYGYGYGYGYEYEYEYENRKETESGAAGSTASSIVLAGGTSNATTIITVAGTGTTVPSITGNGGSGSGSGCGNGGSVRFTDSGNGDSDSNSGVCVTGLTVGGVVGVEFRDVNSDLKVPENFEEVDSQRDVNERTEFGETEFQDPYTGTCIETPVVDLATTTTTTTATTTTTITIIEHNDRTNTTHGVSAPCYEEDQEEDNEEDDDNMVAAMNEHLSTTSATSGIRSSTVSTTSTTGNSVVNQNNSCSRNEFQNVEEDSKPAAGILSFPCDFDHMYASMTDGGAPAAATATSALGVSPLRGNEPRQNDLTEVKHLKELLLLHLDLIQQQSEQIVTKDKLLAALRQENETLKLRLERMDRRVNLQKLRSENSENSITPEHIGACSPPTVNSVNVPSTSELHRNNQSENNNSQYNESFKIRLNTSGGITTVKQEPNENQSKHETKIETGNDARLDWEDKKKRRTDPIPLSTGSKRKRGVSCSSTISNDTSSTVHDKTLTQESGRKSDRKGKSITKKDSFLTTEEHYYTAVGDPNYTLSLKEPNPVESDTSLEVPNWRVKIYTSCYTMEGTENLDDEIFNKRHLKLENDERRRKRWDVQRIREQRHIEKLKQRQERQNHQATCYNTNHTGPCPSTVEEESISSLWPDVEQIQSLQVDPQLPVTAFGAPIPSFTPSEFSLPWLNMSRSSCRRPKRSTGRRKPTRR

Target ORF information:

RefSeq Version	XM_003700437.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata male-specific lethal 1 homolog (LOC100882830), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003700437.2

ORF Insert Sequence:

ATGTCGAGTG GCTCGGCCTC GAAACTCGTG AACGGTGAAA ACAAGAAGGA GAAGGTTCAC 
GGGTACGGGT ACGGGTACGG TTACGGGTAC GGGTACGGGT ACGAATACGA GTACGAGTAC 
GAGAACAGGA AAGAAACCGA GAGCGGCGCT GCTGGCAGCA CTGCCTCTTC CATAGTTCTC 
GCTGGTGGTA CTAGTAACGC TACTACTATC ATTACTGTTG CTGGTACTGG TACTACTGTT 
CCTAGTATTA CTGGTAACGG CGGTAGTGGT AGTGGTAGTG GTTGTGGTAA CGGTGGTAGT 
GTTCGTTTCA CCGATAGTGG TAACGGTGAC AGTGATAGTA ATAGCGGTGT TTGCGTTACC 
GGCTTGACCG TCGGGGGCGT CGTCGGCGTG GAGTTTCGCG ACGTTAATTC CGATCTGAAA 
GTACCAGAGA ATTTCGAAGA GGTAGACAGT CAGAGGGATG TAAACGAGAG GACGGAATTC 
GGTGAAACCG AGTTTCAGGA CCCGTACACG GGAACGTGTA TCGAGACGCC AGTCGTCGAT 
CTCGCTACTA CTACTACCAC CACCACCGCC ACAACCACCA CCACCATCAC CATCATCGAA 
CACAACGATC GAACGAATAC GACCCACGGT GTTTCTGCTC CTTGTTACGA AGAAGATCAG 
GAAGAGGATA ACGAGGAGGA CGACGACAAC ATGGTCGCGG CGATGAACGA ACACTTGTCT 
ACCACGTCGG CGACCAGCGG CATTCGAAGT AGCACTGTTT CGACAACCAG TACCACTGGT 
AATTCCGTCG TCAACCAAAA TAACAGTTGC TCTCGTAATG AATTTCAAAA CGTTGAGGAG 
GATAGTAAAC CGGCCGCTGG AATACTCAGT TTTCCCTGTG ATTTCGATCA CATGTATGCT 
AGCATGACAG ACGGCGGAGC TCCGGCAGCA GCCACTGCCA CTTCTGCTCT GGGTGTCAGT 
CCTCTTCGAG GTAATGAACC GCGTCAAAAC GATCTTACCG AGGTTAAACA CCTCAAGGAA 
CTTTTGCTTC TTCATTTGGA TTTAATTCAA CAACAATCCG AACAAATTGT CACCAAGGAT 
AAACTCTTAG CTGCTCTACG ACAGGAAAAC GAAACGCTTA AGTTGCGTTT GGAGCGTATG 
GATAGACGTG TAAATTTACA AAAGTTACGA TCAGAGAACA GCGAAAACTC CATTACACCA 
GAACATATAG GAGCATGTTC TCCTCCAACT GTTAATTCTG TGAATGTGCC ATCGACTAGT 
GAACTTCACA GGAATAATCA GTCTGAAAAT AATAATTCCC AATACAATGA AAGTTTTAAA 
ATACGTTTAA ATACTAGCGG CGGAATTACT ACGGTTAAAC AAGAACCAAA CGAGAATCAA 
AGTAAGCACG AGACTAAAAT AGAAACTGGC AATGATGCGA GGTTAGATTG GGAAGACAAA 
AAGAAAAGAA GAACAGATCC GATACCATTG TCCACTGGAA GTAAAAGAAA AAGAGGTGTT 
TCGTGTTCGT CTACGATTAG TAATGATACA TCGTCAACGG TACACGATAA GACGTTAACT 
CAGGAGAGCG GTAGGAAAAG TGATAGAAAA GGAAAGAGTA TAACAAAAAA GGATTCTTTT 
CTTACAACAG AGGAACATTA TTACACGGCT GTTGGTGATC CGAATTATAC CTTAAGTTTA 
AAAGAACCTA ATCCTGTGGA AAGTGATACT TCGTTAGAAG TTCCAAATTG GAGGGTAAAG 
ATATATACAA GTTGTTATAC AATGGAAGGA ACTGAAAATT TGGACGACGA AATTTTTAAC 
AAAAGACATT TAAAATTAGA AAATGACGAA AGAAGGAGAA AAAGATGGGA TGTACAAAGG 
ATACGAGAAC AGAGGCACAT CGAAAAGTTG AAACAAAGAC AGGAACGGCA GAATCATCAA 
GCTACATGTT ATAATACAAA TCATACAGGA CCTTGTCCAT CAACGGTCGA GGAAGAGTCT 
ATATCATCCT TGTGGCCTGA TGTAGAACAG ATACAAAGTC TTCAAGTGGA TCCACAATTA 
CCTGTTACTG CATTTGGTGC TCCTATTCCT AGTTTTACTC CAAGTGAATT TTCTTTACCA 
TGGTTAAATA TGTCACGATC GAGTTGCCGG CGTCCAAAGC GATCAACAGG TAGAAGAAAA 
CCTACTAGGA GATAA