LOC100882295 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100882295
Entrez Gene ID	100882295
Full Name	actin-related protein 5
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003704001.2	XP_003704049.1	actin-related protein 5

Pathways from BioSystems
Gene Ontology

The following LOC100882295 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100882295 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe106137	XM_003704001.2 Latest version!	Megachile rotundata actin-related protein 5 (LOC100882295), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100882295 ( XM_003704001.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003704049.1 Megachile rotundata actin-related protein 5 (LOC100882295), mRNA.

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CloneID	OMe106137
Clone ID Related Accession (Same CDS sequence)	XM_003704001.2
Accession Version	XM_003704001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	actin-related protein 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797186.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003704001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATTC TAGAATTGAA AGATGTAAAA GCAGTTCCAG ACATAATTCA TCCTTATCCA 
GAGAGAGTAA AAACTGAAGC AACACCTCTT GTTATTGATA ATGGATCATA CAACTGTAGG 
GTTGGATGGG CAACGGAGAA AGAGCCTCAG TTAATATTTA AAAATCTTAT TGCAAAGCCA 
AGAAAAGAAC GTGGTAAAAA GGATGGAGAA CCTCAGGTTG GAAATGACAT AGCAAACATT 
GAAGCTGTCA GATTCCAGTT GAAAACACAA TTTGACCGCA ATGTAGTAAC TCACTTTGAA 
GCCCAGGAAC AAATATTTGA TTATACCTTC ACTCATATGG GAATAGATAC AGATGGAGCA 
GTAAATCATC CAATTATCTT AACTGAAGCA TTTCTTAATC CTAACTATTC AAGAAATTTG 
ATGGCAGAAC TTTTATTTGA ATGTTATAAT GTACCAGCAA TTGCATATGG AGTAGATTGT 
TTATTTTCAT ACCAACACAA CAACTGTCCT CCAGATGGTT TAATAATTAG TATTGGATAC 
CATACTACTC ATATAATACC TATATTAGAT GGTAAAGCAG ATCCTGTTAA TTCAAGGAGG 
ATCAATGTTG GTGGATATCA TATTACATCT TACATGCACA GATTACTTCA GCTTAAATAT 
CCAGTGCATG TGAATGCTAT AACACCTAGT CGAGCAGAGG AATTAATTCA TGAACATTCA 
ACGATAGCTT TAAATTATCA AGAGGAAATT TCTAAATGGG CAGACCCAGA TCATTACGAT 
GCAAATGTAT TGAGGGTACA ATTACCTTAC ATTGCTCCTG CAAATGCTCC TGGTTTGACA 
GTGGAACAAC AGAAAGAGAG AAAACGCGAG TTAGCTAGGA GATTAATGGA AATAAATGCA 
AGGAAAAGAG AAGAGAGATT GGCAGAAGAT GAAGAGCAGT TGAATCAATT GTTAGCCGTC 
CAAGATCTAT TAGAGGAAGG TGAAACAGAC GAATTTGATC AAGCGTTAAA AACTTACTCA 
CTAGCGAATG AAGCGGATTT GATAAAAATG ATTAATAACT TGCAAGCCAA GGTAGAAAGA 
ACTAGACAAA AAATAGTAGC GGCTAATTCG CAAGAGGAAA ATATCACGAT GGAAGAACAG 
AAGCCAAAAA TCAAATCAAG TTTACAACCA AAAGATCAAC AAGATTTTGA TGAGTGGATA 
GCGGGTGTAA GAAAGAAAAG GCAAGAAATA TTAGAAAAGC GCATGGCGAA GAGACAGCGT 
AGACAAGACA TGGCAAAACG TAGAACCGCA GCTGCACAGG AAAGAATGCG TATAATAAGC 
CAGTTAGCAA GAAAAGAGAA ACGTGATGAT GATTTTGGTA TGAGGGATGA AGACTGGGAC 
GTTTATAAAG TTATAAATAG GGAAGGTGGA GATTCAGATT CAGAGCTGGA ACAAGAAAAG 
CTGCTCGAAC TAGAAGACGT GTTACGCCAC CACGATCCAG AATTCGACGG AGCTGGATCA 
AACGTCCCCA TAGTCCCCGG AGAAACTCAT CAGTTACACG TAGGAGTAGA ATGCCTAAGA 
GCTCCAGAAA TATTGTTCCA ACCATCCATG ATCGGTTCCG TAGAAGCAGG CATTGCTGAA 
ACAATCGAAT TCGTCTTAAA ACTATACCCA CCAGAATTAC AATCTCGTCT CGTAGGAAAC 
ATATTCCTCA CCGGTGGTCC AACAAAATTT CCCGGCCTAT TAGAGAGACT GAATCGCGAG 
CTACGCGAAA TCAGGCCGTT CGGTTCCAAT TTTCAAATAA ACATCGCCAA AAACTCCAGC 
ATAGACGCGT GGTACGGTGC TAGGGACTTT GGCTTGAATG GAAACCTCCC GGAATATTTA 
GTAAGCAAGA AGGAGTACGA AGAGAGGGGT GGTGAATATT TTAAAGAACA TTTAACCAGT 
AATACTTACA CTCGGTCTCC TGACCCGTTG CCTACGGTGC AGGCTCCGGT GACGTCGGAG 
CAGGTTGTTG TCGAGGATGC TGTTGTCGAT GTTGAAATGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003704049.1
CDS	319..2343
Translation	MEILELKDVKAVPDIIHPYPERVKTEATPLVIDNGSYNCRVGWATEKEPQLIFKNLIAKPRKERGKKDGEPQVGNDIANIEAVRFQLKTQFDRNVVTHFEAQEQIFDYTFTHMGIDTDGAVNHPIILTEAFLNPNYSRNLMAELLFECYNVPAIAYGVDCLFSYQHNNCPPDGLIISIGYHTTHIIPILDGKADPVNSRRINVGGYHITSYMHRLLQLKYPVHVNAITPSRAEELIHEHSTIALNYQEEISKWADPDHYDANVLRVQLPYIAPANAPGLTVEQQKERKRELARRLMEINARKREERLAEDEEQLNQLLAVQDLLEEGETDEFDQALKTYSLANEADLIKMINNLQAKVERTRQKIVAANSQEENITMEEQKPKIKSSLQPKDQQDFDEWIAGVRKKRQEILEKRMAKRQRRQDMAKRRTAAAQERMRIISQLARKEKRDDDFGMRDEDWDVYKVINREGGDSDSELEQEKLLELEDVLRHHDPEFDGAGSNVPIVPGETHQLHVGVECLRAPEILFQPSMIGSVEAGIAETIEFVLKLYPPELQSRLVGNIFLTGGPTKFPGLLERLNRELREIRPFGSNFQINIAKNSSIDAWYGARDFGLNGNLPEYLVSKKEYEERGGEYFKEHLTSNTYTRSPDPLPTVQAPVTSEQVVVEDAVVDVEME

Target ORF information:

RefSeq Version	XM_003704001.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata actin-related protein 5 (LOC100882295), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003704001.2

ORF Insert Sequence:

ATGGAGATTC TAGAATTGAA AGATGTAAAA GCAGTTCCAG ACATAATTCA TCCTTATCCA 
GAGAGAGTAA AAACTGAAGC AACACCTCTT GTTATTGATA ATGGATCATA CAACTGTAGG 
GTTGGATGGG CAACGGAGAA AGAGCCTCAG TTAATATTTA AAAATCTTAT TGCAAAGCCA 
AGAAAAGAAC GTGGTAAAAA GGATGGAGAA CCTCAGGTTG GAAATGACAT AGCAAACATT 
GAAGCTGTCA GATTCCAGTT GAAAACACAA TTTGACCGCA ATGTAGTAAC TCACTTTGAA 
GCCCAGGAAC AAATATTTGA TTATACCTTC ACTCATATGG GAATAGATAC AGATGGAGCA 
GTAAATCATC CAATTATCTT AACTGAAGCA TTTCTTAATC CTAACTATTC AAGAAATTTG 
ATGGCAGAAC TTTTATTTGA ATGTTATAAT GTACCAGCAA TTGCATATGG AGTAGATTGT 
TTATTTTCAT ACCAACACAA CAACTGTCCT CCAGATGGTT TAATAATTAG TATTGGATAC 
CATACTACTC ATATAATACC TATATTAGAT GGTAAAGCAG ATCCTGTTAA TTCAAGGAGG 
ATCAATGTTG GTGGATATCA TATTACATCT TACATGCACA GATTACTTCA GCTTAAATAT 
CCAGTGCATG TGAATGCTAT AACACCTAGT CGAGCAGAGG AATTAATTCA TGAACATTCA 
ACGATAGCTT TAAATTATCA AGAGGAAATT TCTAAATGGG CAGACCCAGA TCATTACGAT 
GCAAATGTAT TGAGGGTACA ATTACCTTAC ATTGCTCCTG CAAATGCTCC TGGTTTGACA 
GTGGAACAAC AGAAAGAGAG AAAACGCGAG TTAGCTAGGA GATTAATGGA AATAAATGCA 
AGGAAAAGAG AAGAGAGATT GGCAGAAGAT GAAGAGCAGT TGAATCAATT GTTAGCCGTC 
CAAGATCTAT TAGAGGAAGG TGAAACAGAC GAATTTGATC AAGCGTTAAA AACTTACTCA 
CTAGCGAATG AAGCGGATTT GATAAAAATG ATTAATAACT TGCAAGCCAA GGTAGAAAGA 
ACTAGACAAA AAATAGTAGC GGCTAATTCG CAAGAGGAAA ATATCACGAT GGAAGAACAG 
AAGCCAAAAA TCAAATCAAG TTTACAACCA AAAGATCAAC AAGATTTTGA TGAGTGGATA 
GCGGGTGTAA GAAAGAAAAG GCAAGAAATA TTAGAAAAGC GCATGGCGAA GAGACAGCGT 
AGACAAGACA TGGCAAAACG TAGAACCGCA GCTGCACAGG AAAGAATGCG TATAATAAGC 
CAGTTAGCAA GAAAAGAGAA ACGTGATGAT GATTTTGGTA TGAGGGATGA AGACTGGGAC 
GTTTATAAAG TTATAAATAG GGAAGGTGGA GATTCAGATT CAGAGCTGGA ACAAGAAAAG 
CTGCTCGAAC TAGAAGACGT GTTACGCCAC CACGATCCAG AATTCGACGG AGCTGGATCA 
AACGTCCCCA TAGTCCCCGG AGAAACTCAT CAGTTACACG TAGGAGTAGA ATGCCTAAGA 
GCTCCAGAAA TATTGTTCCA ACCATCCATG ATCGGTTCCG TAGAAGCAGG CATTGCTGAA 
ACAATCGAAT TCGTCTTAAA ACTATACCCA CCAGAATTAC AATCTCGTCT CGTAGGAAAC 
ATATTCCTCA CCGGTGGTCC AACAAAATTT CCCGGCCTAT TAGAGAGACT GAATCGCGAG 
CTACGCGAAA TCAGGCCGTT CGGTTCCAAT TTTCAAATAA ACATCGCCAA AAACTCCAGC 
ATAGACGCGT GGTACGGTGC TAGGGACTTT GGCTTGAATG GAAACCTCCC GGAATATTTA 
GTAAGCAAGA AGGAGTACGA AGAGAGGGGT GGTGAATATT TTAAAGAACA TTTAACCAGT 
AATACTTACA CTCGGTCTCC TGACCCGTTG CCTACGGTGC AGGCTCCGGT GACGTCGGAG 
CAGGTTGTTG TCGAGGATGC TGTTGTCGAT GTTGAAATGG AATAA