LOC100882136 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100882136
Entrez Gene ID	100882136
Full Name	bipolar kinesin KRP-130
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012279659.1	XP_012135049.1	bipolar kinesin KRP-130 isoform X1
XM_012279660.1	XP_012135050.1	bipolar kinesin KRP-130 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100882136 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100882136 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe99701	XM_012279659.1 Latest version!	Megachile rotundata bipolar kinesin KRP-130 (LOC100882136), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe99702	XM_012279660.1 Latest version!	Megachile rotundata bipolar kinesin KRP-130 (LOC100882136), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100882136 ( XM_012279659.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012135049.1 Megachile rotundata bipolar kinesin KRP-130 (LOC100882136), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe99701
Clone ID Related Accession (Same CDS sequence)	XM_012279659.1
Accession Version	XM_012279659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	bipolar kinesin KRP-130 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797102.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAAAATAG TAGAAGAGAA AAATCTCAGC ATGTACAAGT ATTTGTCCGT 
GTTAGGCCAA CAAATGCTGT TGAAAAAAAT AATAAGTCCA AAACTATTGT AGAAGTTGCT 
AATGATAGAG AATTAATAGT AAAATATCCA CATGATAAAG TTTCTAAGAA ATATAAATTT 
GACAATGTAT TTGGACCTTC TGCAAGACAG ATCGATATAT ATAATGTTGT TGTTAATCCA 
TTATTGGAAC AAGTTTTGGC TGGATATAAT TGTACAGTGT TTGCATATGG TCAAACTGGT 
ACAGGAAAAA CATTTACTAT GGAAGGAAAT AATAGTGATC CAACTTTGCA TTGGCAAAAT 
GATTCATCTG CTGGTATAAT ACCTCGTTCT TTAAGTCATC TATTTGATAC ATTACGTGTA 
TTGGAAATAA AAGAGTACAG AATTAGAGTT AGTTTCTTGG AACTCTATAA TGAGAATTTA 
TTTGATCTAT TATCAATTAA TGATGATTGT TCTAAAATAA AATTATATGA AGATGCATCA 
AAAAAAGGTA CTATAACTAT TCATGGTTTA GAAGAAGTAA GAGTACGTAA TAAAAGTGAA 
GTTTATAAAA TTCTGGAAAA GGGATCAGAG AGGAGAAAAA CTGCTGCAAC GCTGTTAAAC 
AGTCAATCTA GTCGTTCTCA TACTGTATTT TCGATTACTG TACACATGAA AGAGAATACC 
ATTGACGGAG AAGAAGTTTT AAAAACGGGA AAGTTAAATT TGGTAGATTT AGCAGGAAGC 
GAAAGTATCG GGAGATCTGG CGCTATTGAA AAGAGGGCAA GAGAAGCTGG TAGTATTAAT 
CAGTCCTTAT TAACCCTTGG TAGAGTTATA ACAGCTCTTG TTGAAAGAGC ACCTCATGTA 
CCTTATAGAG AATCCAAACT CACTAGGTTG CTACAAGAGT CACTGGGTGG TCGAACAAAA 
ACCTCTATTA TCGCTACAAT ATCTCCTGCT GCTATTAATC TCGAAGAAAC GCTATCAACA 
TTAGATTATG CACATCGAGC GAGAAACATT ACTAATCGTC CAGAAATAAA TCAAAAACTT 
TCAAAAAAAG AATTCCTTAA ACAGTATAGA GTTGAAATCG AAAGATTACG AAAAGATTTA 
GAAGCAACGC GTGAGGAGAA TGGAGTTTGT GTCGCTAATG AGAATTATCA AGAAATGCAG 
ACAATGATCG CTCAATTAAA TAAAGATATA AAAGAGAAAA TAAATCACAT AAAAGCGCTT 
GAAAAAACTG TGCAAGATAA GGAGGAAATA TACAATGATC TTGAAGTACA AACTCTTGCT 
CAAACGCAAG AATTCTACAA AATTAAAAAT AAATTAGATA ATGTTACGAG TGCCCTTAAA 
TCTACAGATG AAGGGTTAAA AATGACGATT GAGGAACGAG ATACACAGAA ATTCTTACTA 
GAAAAATATG TTAATACAGA ACAATCTCTA TTAAATCAAG CTCAAAATTT ATTAAATATT 
GCAGATACAG CAACGTCAGA TTTGTATAAG TTACATGATA AGATTGAACG TCAAACGCAA 
ACTGAACAAA CGTTTAAAGC CCTTAGTGAA CAGTTCAGAA ACAATGTTTC TTATTGTGTA 
CAAGGAATGG AGAAGGATGT TATTGCGTGT GTAAAAAGAT TAATAAATTG TTTTACATCT 
ATAAAGAATG ATATTGATCT GTATGCTAAC AATACATGTG AAAGTGTAAA CACAGCAATA 
CAACAAATTG CAATGGATCT TATTAAACAA CATTTATCCA ATATTGATAA TTTAAAGAAG 
AGTATAAACG AATTTTATTC AAATGACCAA AAGTGCATAA AAGATAAATC AACTGTAATT 
CCAGTGAGTA CAGTAGAATG TAAACATGAA TTATTAAATA AAATTTCTAT AACATTGGTA 
AATGATATTC AAAATATAAT GAGAAATGAA ATTGTTAAAA ATTTGCAAAT AATAAGGAAT 
AATGTGACCC AAAAATTAAA GCGAACTTCA ATTCTTATGA AAGAAATGAT AGATTCTGCA 
TGTTATGTTC AACTAGAAGC TTACAATGAT ATAAATAAAA ACATTCAAAT TATTGCAAAT 
GATGTAGAAA TGCTCGATAA AAGGCAACAG TTTACAGAAA AACAAAAATT ATTCGCAAAG 
ATGATGCAAG ATGCAGTTTC TCAATTCAAC CATTTGTATA AGAGTCAAGA GGAGCACTAT 
TCTTCTGTAA CTGACAAATG TAATTATGTC AGCAAAATTT GCAATGATAT CACTGATTAT 
TCAACAAAAA GTATCGCGTT GCAAAACGAT CTAAAAGATC AAATACAACG TAACTTACAA 
AAAATTGAAA CAAATGTCAT TCTTAAAACA GAAGAGAGTG AAGAGTTAGC AGATAAAACA 
GCAAAGCAAG GAAAAATTCT AATTAACGCA TTAGAATTAA ATATAAACGA AAGCTGTAAC 
ATATTGAAAC AATATAAAGA CCTTACTGAG AATAGCGTTA AAGAGGTACA ACAGAAATTT 
GATGTGGATA GAAATAAAGC TCTTTTATTG GTTAATAATG CTCAGGAAAT AATTACGAAT 
GTAAGTAAAG ATGCAGGATT AATGAAAGGC CAACAAATTA AAATAACTGA AATCTCTAAC 
AAAATAATTG GTAAATTAGA GGATCAAAGT GCAGAATCAA GTGCATGGAG TGATAGAATT 
CCTAATCAGT TAAAATCAAT AGTGGGCGAT GTTAATAAAT TTTTTGATGA AGATTTACAA 
CGTGATGTTC CAACAGGAAC AACTCCTGCT AGGAGAGAAT TTTCTTATCC TCGTCAATTT 
ATTGCAACAT CTCCTCATGA ACAAATTCTT CAAAGATTCA GACAAGCTAG GAAACCTATT 
GAAACGTCGG AAGATGATAG TATCCTGTCT AAACCATATT CTACAGTTAA TGATGAAATA 
GTTATTAATT CTGATAACAA GCCAAGTAAA AAAATACACA AAGCTATGCA AGTTTATTTC 
CAACAAGCTG AGGAATATAA GGAATTTATA CAAAAACAAA TAGCAGAATA TGATATTGGA 
AAACGACATC TAGCTAATAT GATGGGCGAA GATCCTGAGA ATTTTTCTGA GGCAGATATT 
AATAGATCAA TAAAATATTT ATTTCCATCT GGTTTATATG AACCAGAAGC TCGTCCTATG 
ATAATTCATC CTAGTAAATT ATATGCAAGC AGAAAAGAAG CTGAATTTGA TGAAAGTGGC 
AGACCACATC ACTTCCTATA TTATACTACA AAACCTAACT ATTATAACAT ACTTCATAAA 
ATTGCAGAAA ATATGATTAA ATTAAATGAG CTGGAGGATA AGACCGCACA AATAAAGAAA 
TACAGTACCA TAGAGGATAA ATTTGATGTT GCAAAAACAG AATGGCTAAA CAAAACTGAA 
TTAGAGGAGA AATTATTGGA AGAACTCTCT GATGCACAAT ATGAATACTT TCTAACAAGT 
ATAGAAAGAG TGCTTCAACA TCCCTTTTCA AATCATGCAT CTTCTTTGCT CAACGAATGT 
AGAAAACAGA TAACTGCGAT TTCTACTACC ATGAAGCTCC CACTAGTACA ATTTGATGAG 
AATAATAGAC CTTATATTTT ATTAAAGAAT TGTGCAAGGA AATGCGCACG AGGCGACGTT 
AAAGTGATAG GTAATGGAAC TGGAAACATT ACTATTAATG GAGAAGACAT TTCATATTTT 
ACGGATATAC AGTGTCGTGA GCAGGTTATA TTTCCATTAA TATTTTCGGA TATGAGCGAC 
AAAGTTGATG TTGAAGTAAC CGTTTCTGGT GGGGGACCAA CTGGACAAGC AGGCCTCATT 
CGTTGGGGTA TTGCAAAGGC GCTTCGAAAT TTTGTCAGTA AGGAAATGAT AGAAAAAATG 
AGAATTGCTG GACTTCTAAC ACACGATATG AGGCGCCGCG AAAGGAAGAA GTTTGGCCAG 
GAAGGAGCTC GAAGAAAGTT CACATGGAAG AAACGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012135049.1
CDS	1290..5288
Translation	MNEKKNSRREKSQHVQVFVRVRPTNAVEKNNKSKTIVEVANDRELIVKYPHDKVSKKYKFDNVFGPSARQIDIYNVVVNPLLEQVLAGYNCTVFAYGQTGTGKTFTMEGNNSDPTLHWQNDSSAGIIPRSLSHLFDTLRVLEIKEYRIRVSFLELYNENLFDLLSINDDCSKIKLYEDASKKGTITIHGLEEVRVRNKSEVYKILEKGSERRKTAATLLNSQSSRSHTVFSITVHMKENTIDGEEVLKTGKLNLVDLAGSESIGRSGAIEKRAREAGSINQSLLTLGRVITALVERAPHVPYRESKLTRLLQESLGGRTKTSIIATISPAAINLEETLSTLDYAHRARNITNRPEINQKLSKKEFLKQYRVEIERLRKDLEATREENGVCVANENYQEMQTMIAQLNKDIKEKINHIKALEKTVQDKEEIYNDLEVQTLAQTQEFYKIKNKLDNVTSALKSTDEGLKMTIEERDTQKFLLEKYVNTEQSLLNQAQNLLNIADTATSDLYKLHDKIERQTQTEQTFKALSEQFRNNVSYCVQGMEKDVIACVKRLINCFTSIKNDIDLYANNTCESVNTAIQQIAMDLIKQHLSNIDNLKKSINEFYSNDQKCIKDKSTVIPVSTVECKHELLNKISITLVNDIQNIMRNEIVKNLQIIRNNVTQKLKRTSILMKEMIDSACYVQLEAYNDINKNIQIIANDVEMLDKRQQFTEKQKLFAKMMQDAVSQFNHLYKSQEEHYSSVTDKCNYVSKICNDITDYSTKSIALQNDLKDQIQRNLQKIETNVILKTEESEELADKTAKQGKILINALELNINESCNILKQYKDLTENSVKEVQQKFDVDRNKALLLVNNAQEIITNVSKDAGLMKGQQIKITEISNKIIGKLEDQSAESSAWSDRIPNQLKSIVGDVNKFFDEDLQRDVPTGTTPARREFSYPRQFIATSPHEQILQRFRQARKPIETSEDDSILSKPYSTVNDEIVINSDNKPSKKIHKAMQVYFQQAEEYKEFIQKQIAEYDIGKRHLANMMGEDPENFSEADINRSIKYLFPSGLYEPEARPMIIHPSKLYASRKEAEFDESGRPHHFLYYTTKPNYYNILHKIAENMIKLNELEDKTAQIKKYSTIEDKFDVAKTEWLNKTELEEKLLEELSDAQYEYFLTSIERVLQHPFSNHASSLLNECRKQITAISTTMKLPLVQFDENNRPYILLKNCARKCARGDVKVIGNGTGNITINGEDISYFTDIQCREQVIFPLIFSDMSDKVDVEVTVSGGGPTGQAGLIRWGIAKALRNFVSKEMIEKMRIAGLLTHDMRRRERKKFGQEGARRKFTWKKR

Target ORF information:

RefSeq Version	XM_012279659.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata bipolar kinesin KRP-130 (LOC100882136), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012279659.1

ORF Insert Sequence:

ATGAATGAGA AAAAAAATAG TAGAAGAGAA AAATCTCAGC ATGTACAAGT ATTTGTCCGT 
GTTAGGCCAA CAAATGCTGT TGAAAAAAAT AATAAGTCCA AAACTATTGT AGAAGTTGCT 
AATGATAGAG AATTAATAGT AAAATATCCA CATGATAAAG TTTCTAAGAA ATATAAATTT 
GACAATGTAT TTGGACCTTC TGCAAGACAG ATCGATATAT ATAATGTTGT TGTTAATCCA 
TTATTGGAAC AAGTTTTGGC TGGATATAAT TGTACAGTGT TTGCATATGG TCAAACTGGT 
ACAGGAAAAA CATTTACTAT GGAAGGAAAT AATAGTGATC CAACTTTGCA TTGGCAAAAT 
GATTCATCTG CTGGTATAAT ACCTCGTTCT TTAAGTCATC TATTTGATAC ATTACGTGTA 
TTGGAAATAA AAGAGTACAG AATTAGAGTT AGTTTCTTGG AACTCTATAA TGAGAATTTA 
TTTGATCTAT TATCAATTAA TGATGATTGT TCTAAAATAA AATTATATGA AGATGCATCA 
AAAAAAGGTA CTATAACTAT TCATGGTTTA GAAGAAGTAA GAGTACGTAA TAAAAGTGAA 
GTTTATAAAA TTCTGGAAAA GGGATCAGAG AGGAGAAAAA CTGCTGCAAC GCTGTTAAAC 
AGTCAATCTA GTCGTTCTCA TACTGTATTT TCGATTACTG TACACATGAA AGAGAATACC 
ATTGACGGAG AAGAAGTTTT AAAAACGGGA AAGTTAAATT TGGTAGATTT AGCAGGAAGC 
GAAAGTATCG GGAGATCTGG CGCTATTGAA AAGAGGGCAA GAGAAGCTGG TAGTATTAAT 
CAGTCCTTAT TAACCCTTGG TAGAGTTATA ACAGCTCTTG TTGAAAGAGC ACCTCATGTA 
CCTTATAGAG AATCCAAACT CACTAGGTTG CTACAAGAGT CACTGGGTGG TCGAACAAAA 
ACCTCTATTA TCGCTACAAT ATCTCCTGCT GCTATTAATC TCGAAGAAAC GCTATCAACA 
TTAGATTATG CACATCGAGC GAGAAACATT ACTAATCGTC CAGAAATAAA TCAAAAACTT 
TCAAAAAAAG AATTCCTTAA ACAGTATAGA GTTGAAATCG AAAGATTACG AAAAGATTTA 
GAAGCAACGC GTGAGGAGAA TGGAGTTTGT GTCGCTAATG AGAATTATCA AGAAATGCAG 
ACAATGATCG CTCAATTAAA TAAAGATATA AAAGAGAAAA TAAATCACAT AAAAGCGCTT 
GAAAAAACTG TGCAAGATAA GGAGGAAATA TACAATGATC TTGAAGTACA AACTCTTGCT 
CAAACGCAAG AATTCTACAA AATTAAAAAT AAATTAGATA ATGTTACGAG TGCCCTTAAA 
TCTACAGATG AAGGGTTAAA AATGACGATT GAGGAACGAG ATACACAGAA ATTCTTACTA 
GAAAAATATG TTAATACAGA ACAATCTCTA TTAAATCAAG CTCAAAATTT ATTAAATATT 
GCAGATACAG CAACGTCAGA TTTGTATAAG TTACATGATA AGATTGAACG TCAAACGCAA 
ACTGAACAAA CGTTTAAAGC CCTTAGTGAA CAGTTCAGAA ACAATGTTTC TTATTGTGTA 
CAAGGAATGG AGAAGGATGT TATTGCGTGT GTAAAAAGAT TAATAAATTG TTTTACATCT 
ATAAAGAATG ATATTGATCT GTATGCTAAC AATACATGTG AAAGTGTAAA CACAGCAATA 
CAACAAATTG CAATGGATCT TATTAAACAA CATTTATCCA ATATTGATAA TTTAAAGAAG 
AGTATAAACG AATTTTATTC AAATGACCAA AAGTGCATAA AAGATAAATC AACTGTAATT 
CCAGTGAGTA CAGTAGAATG TAAACATGAA TTATTAAATA AAATTTCTAT AACATTGGTA 
AATGATATTC AAAATATAAT GAGAAATGAA ATTGTTAAAA ATTTGCAAAT AATAAGGAAT 
AATGTGACCC AAAAATTAAA GCGAACTTCA ATTCTTATGA AAGAAATGAT AGATTCTGCA 
TGTTATGTTC AACTAGAAGC TTACAATGAT ATAAATAAAA ACATTCAAAT TATTGCAAAT 
GATGTAGAAA TGCTCGATAA AAGGCAACAG TTTACAGAAA AACAAAAATT ATTCGCAAAG 
ATGATGCAAG ATGCAGTTTC TCAATTCAAC CATTTGTATA AGAGTCAAGA GGAGCACTAT 
TCTTCTGTAA CTGACAAATG TAATTATGTC AGCAAAATTT GCAATGATAT CACTGATTAT 
TCAACAAAAA GTATCGCGTT GCAAAACGAT CTAAAAGATC AAATACAACG TAACTTACAA 
AAAATTGAAA CAAATGTCAT TCTTAAAACA GAAGAGAGTG AAGAGTTAGC AGATAAAACA 
GCAAAGCAAG GAAAAATTCT AATTAACGCA TTAGAATTAA ATATAAACGA AAGCTGTAAC 
ATATTGAAAC AATATAAAGA CCTTACTGAG AATAGCGTTA AAGAGGTACA ACAGAAATTT 
GATGTGGATA GAAATAAAGC TCTTTTATTG GTTAATAATG CTCAGGAAAT AATTACGAAT 
GTAAGTAAAG ATGCAGGATT AATGAAAGGC CAACAAATTA AAATAACTGA AATCTCTAAC 
AAAATAATTG GTAAATTAGA GGATCAAAGT GCAGAATCAA GTGCATGGAG TGATAGAATT 
CCTAATCAGT TAAAATCAAT AGTGGGCGAT GTTAATAAAT TTTTTGATGA AGATTTACAA 
CGTGATGTTC CAACAGGAAC AACTCCTGCT AGGAGAGAAT TTTCTTATCC TCGTCAATTT 
ATTGCAACAT CTCCTCATGA ACAAATTCTT CAAAGATTCA GACAAGCTAG GAAACCTATT 
GAAACGTCGG AAGATGATAG TATCCTGTCT AAACCATATT CTACAGTTAA TGATGAAATA 
GTTATTAATT CTGATAACAA GCCAAGTAAA AAAATACACA AAGCTATGCA AGTTTATTTC 
CAACAAGCTG AGGAATATAA GGAATTTATA CAAAAACAAA TAGCAGAATA TGATATTGGA 
AAACGACATC TAGCTAATAT GATGGGCGAA GATCCTGAGA ATTTTTCTGA GGCAGATATT 
AATAGATCAA TAAAATATTT ATTTCCATCT GGTTTATATG AACCAGAAGC TCGTCCTATG 
ATAATTCATC CTAGTAAATT ATATGCAAGC AGAAAAGAAG CTGAATTTGA TGAAAGTGGC 
AGACCACATC ACTTCCTATA TTATACTACA AAACCTAACT ATTATAACAT ACTTCATAAA 
ATTGCAGAAA ATATGATTAA ATTAAATGAG CTGGAGGATA AGACCGCACA AATAAAGAAA 
TACAGTACCA TAGAGGATAA ATTTGATGTT GCAAAAACAG AATGGCTAAA CAAAACTGAA 
TTAGAGGAGA AATTATTGGA AGAACTCTCT GATGCACAAT ATGAATACTT TCTAACAAGT 
ATAGAAAGAG TGCTTCAACA TCCCTTTTCA AATCATGCAT CTTCTTTGCT CAACGAATGT 
AGAAAACAGA TAACTGCGAT TTCTACTACC ATGAAGCTCC CACTAGTACA ATTTGATGAG 
AATAATAGAC CTTATATTTT ATTAAAGAAT TGTGCAAGGA AATGCGCACG AGGCGACGTT 
AAAGTGATAG GTAATGGAAC TGGAAACATT ACTATTAATG GAGAAGACAT TTCATATTTT 
ACGGATATAC AGTGTCGTGA GCAGGTTATA TTTCCATTAA TATTTTCGGA TATGAGCGAC 
AAAGTTGATG TTGAAGTAAC CGTTTCTGGT GGGGGACCAA CTGGACAAGC AGGCCTCATT 
CGTTGGGGTA TTGCAAAGGC GCTTCGAAAT TTTGTCAGTA AGGAAATGAT AGAAAAAATG 
AGAATTGCTG GACTTCTAAC ACACGATATG AGGCGCCGCG AAAGGAAGAA GTTTGGCCAG 
GAAGGAGCTC GAAGAAAGTT CACATGGAAG AAACGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882136 ( XM_012279660.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012135050.1 Megachile rotundata bipolar kinesin KRP-130 (LOC100882136), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe99702
Clone ID Related Accession (Same CDS sequence)	XM_012279660.1
Accession Version	XM_012279660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	bipolar kinesin KRP-130 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797102.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTGT TAATCCATTA TTGGAACAAG TTTTGGCTGG ATATAATTGT ACAGTGTTTG 
CATATGGTCA AACTGGTACA GGAAAAACAT TTACTATGGA AGGAAATAAT AGTGATCCAA 
CTTTGCATTG GCAAAATATT ATATGAAGAT GCATCAAAAA AAGGTACTAT AACTATTCAT 
GGTTTAGAAG AAGTAAGAGT ACGTAATAAA AGTGAAGTTT ATAAAATTCT GGAAAAGGGA 
TCAGAGAGGA GAAAAACTGC TGCAACGCTG TTAAACAGTC AATCTAGTCG TTCTCATACT 
GTATTTTCGA TTACTGTACA CATGAAAGAG AATACCATTG ACGGAGAAGA AGTTTTAAAA 
ACGGGAAAGT TAAATTTGGT AGATTTAGCA GGAAGCGAAA GTATCGGGAG ATCTGGCGCT 
ATTGAAAAGA GGGCAAGAGA AGCTGGTAGT ATTAATCAGT CCTTATTAAC CCTTGGTAGA 
GTTATAACAG CTCTTGTTGA AAGAGCACCT CATGTACCTT ATAGAGAATC CAAACTCACT 
AGGTTGCTAC AAGAGTCACT GGGTGGTCGA ACAAAAACCT CTATTATCGC TACAATATCT 
CCTGCTGCTA TTAATCTCGA AGAAACGCTA TCAACATTAG ATTATGCACA TCGAGCGAGA 
AACATTACTA ATCGTCCAGA AATAAATCAA AAACTTTCAA AAAAAGAATT CCTTAAACAG 
TATAGAGTTG AAATCGAAAG ATTACGAAAA GATTTAGAAG CAACGCGTGA GGAGAATGGA 
GTTTGTGTCG CTAATGAGAA TTATCAAGAA ATGCAGACAA TGATCGCTCA ATTAAATAAA 
GATATAAAAG AGAAAATAAA TCACATAAAA GCGCTTGAAA AAACTGTGCA AGATAAGGAG 
GAAATATACA ATGATCTTGA AGTACAAACT CTTGCTCAAA CGCAAGAATT CTACAAAATT 
AAAAATAAAT TAGATAATGT TACGAGTGCC CTTAAATCTA CAGATGAAGG GTTAAAAATG 
ACGATTGAGG AACGAGATAC ACAGAAATTC TTACTAGAAA AATATGTTAA TACAGAACAA 
TCTCTATTAA ATCAAGCTCA AAATTTATTA AATATTGCAG ATACAGCAAC GTCAGATTTG 
TATAAGTTAC ATGATAAGAT TGAACGTCAA ACGCAAACTG AACAAACGTT TAAAGCCCTT 
AGTGAACAGT TCAGAAACAA TGTTTCTTAT TGTGTACAAG GAATGGAGAA GGATGTTATT 
GCGTGTGTAA AAAGATTAAT AAATTGTTTT ACATCTATAA AGAATGATAT TGATCTGTAT 
GCTAACAATA CATGTGAAAG TGTAAACACA GCAATACAAC AAATTGCAAT GGATCTTATT 
AAACAACATT TATCCAATAT TGATAATTTA AAGAAGAGTA TAAACGAATT TTATTCAAAT 
GACCAAAAGT GCATAAAAGA TAAATCAACT GTAATTCCAG TGAGTACAGT AGAATGTAAA 
CATGAATTAT TAAATAAAAT TTCTATAACA TTGGTAAATG ATATTCAAAA TATAATGAGA 
AATGAAATTG TTAAAAATTT GCAAATAATA AGGAATAATG TGACCCAAAA ATTAAAGCGA 
ACTTCAATTC TTATGAAAGA AATGATAGAT TCTGCATGTT ATGTTCAACT AGAAGCTTAC 
AATGATATAA ATAAAAACAT TCAAATTATT GCAAATGATG TAGAAATGCT CGATAAAAGG 
CAACAGTTTA CAGAAAAACA AAAATTATTC GCAAAGATGA TGCAAGATGC AGTTTCTCAA 
TTCAACCATT TGTATAAGAG TCAAGAGGAG CACTATTCTT CTGTAACTGA CAAATGTAAT 
TATGTCAGCA AAATTTGCAA TGATATCACT GATTATTCAA CAAAAAGTAT CGCGTTGCAA 
AACGATCTAA AAGATCAAAT ACAACGTAAC TTACAAAAAA TTGAAACAAA TGTCATTCTT 
AAAACAGAAG AGAGTGAAGA GTTAGCAGAT AAAACAGCAA AGCAAGGAAA AATTCTAATT 
AACGCATTAG AATTAAATAT AAACGAAAGC TGTAACATAT TGAAACAATA TAAAGACCTT 
ACTGAGAATA GCGTTAAAGA GGTACAACAG AAATTTGATG TGGATAGAAA TAAAGCTCTT 
TTATTGGTTA ATAATGCTCA GGAAATAATT ACGAATGTAA GTAAAGATGC AGGATTAATG 
AAAGGCCAAC AAATTAAAAT AACTGAAATC TCTAACAAAA TAATTGGTAA ATTAGAGGAT 
CAAAGTGCAG AATCAAGTGC ATGGAGTGAT AGAATTCCTA ATCAGTTAAA ATCAATAGTG 
GGCGATGTTA ATAAATTTTT TGATGAAGAT TTACAACGTG ATGTTCCAAC AGGAACAACT 
CCTGCTAGGA GAGAATTTTC TTATCCTCGT CAATTTATTG CAACATCTCC TCATGAACAA 
ATTCTTCAAA GATTCAGACA AGCTAGGAAA CCTATTGAAA CGTCGGAAGA TGATAGTATC 
CTGTCTAAAC CATATTCTAC AGTTAATGAT GAAATAGTTA TTAATTCTGA TAACAAGCCA 
AGTAAAAAAA TACACAAAGC TATGCAAGTT TATTTCCAAC AAGCTGAGGA ATATAAGGAA 
TTTATACAAA AACAAATAGC AGAATATGAT ATTGGAAAAC GACATCTAGC TAATATGATG 
GGCGAAGATC CTGAGAATTT TTCTGAGGCA GATATTAATA GATCAATAAA ATATTTATTT 
CCATCTGGTT TATATGAACC AGAAGCTCGT CCTATGATAA TTCATCCTAG TAAATTATAT 
GCAAGCAGAA AAGAAGCTGA ATTTGATGAA AGTGGCAGAC CACATCACTT CCTATATTAT 
ACTACAAAAC CTAACTATTA TAACATACTT CATAAAATTG CAGAAAATAT GATTAAATTA 
AATGAGCTGG AGGATAAGAC CGCACAAATA AAGAAATACA GTACCATAGA GGATAAATTT 
GATGTTGCAA AAACAGAATG GCTAAACAAA ACTGAATTAG AGGAGAAATT ATTGGAAGAA 
CTCTCTGATG CACAATATGA ATACTTTCTA ACAAGTATAG AAAGAGTGCT TCAACATCCC 
TTTTCAAATC ATGCATCTTC TTTGCTCAAC GAATGTAGAA AACAGATAAC TGCGATTTCT 
ACTACCATGA AGCTCCCACT AGTACAATTT GATGAGAATA ATAGACCTTA TATTTTATTA 
AAGAATTGTG CAAGGAAATG CGCACGAGGC GACGTTAAAG TGATAGGTAA TGGAACTGGA 
AACATTACTA TTAATGGAGA AGACATTTCA TATTTTACGG ATATACAGTG TCGTGAGCAG 
GTTATATTTC CATTAATATT TTCGGATATG AGCGACAAAG TTGATGTTGA AGTAACCGTT 
TCTGGTGGGG GACCAACTGG ACAAGCAGGC CTCATTCGTT GGGGTATTGC AAAGGCGCTT 
CGAAATTTTG TCAGTAAGGA AATGATAGAA AAAATGAGAA TTGCTGGACT TCTAACACAC 
GATATGAGGC GCCGCGAAAG GAAGAAGTTT GGCCAGGAAG GAGCTCGAAG AAAGTTCACA 
TGGAAGAAAC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012135050.1
CDS	10..3624
Translation	MLLLIHYWNKFWLDIIVQCLHMVKLVQEKHLLWKEIIVIQLCIGKILYEDASKKGTITIHGLEEVRVRNKSEVYKILEKGSERRKTAATLLNSQSSRSHTVFSITVHMKENTIDGEEVLKTGKLNLVDLAGSESIGRSGAIEKRAREAGSINQSLLTLGRVITALVERAPHVPYRESKLTRLLQESLGGRTKTSIIATISPAAINLEETLSTLDYAHRARNITNRPEINQKLSKKEFLKQYRVEIERLRKDLEATREENGVCVANENYQEMQTMIAQLNKDIKEKINHIKALEKTVQDKEEIYNDLEVQTLAQTQEFYKIKNKLDNVTSALKSTDEGLKMTIEERDTQKFLLEKYVNTEQSLLNQAQNLLNIADTATSDLYKLHDKIERQTQTEQTFKALSEQFRNNVSYCVQGMEKDVIACVKRLINCFTSIKNDIDLYANNTCESVNTAIQQIAMDLIKQHLSNIDNLKKSINEFYSNDQKCIKDKSTVIPVSTVECKHELLNKISITLVNDIQNIMRNEIVKNLQIIRNNVTQKLKRTSILMKEMIDSACYVQLEAYNDINKNIQIIANDVEMLDKRQQFTEKQKLFAKMMQDAVSQFNHLYKSQEEHYSSVTDKCNYVSKICNDITDYSTKSIALQNDLKDQIQRNLQKIETNVILKTEESEELADKTAKQGKILINALELNINESCNILKQYKDLTENSVKEVQQKFDVDRNKALLLVNNAQEIITNVSKDAGLMKGQQIKITEISNKIIGKLEDQSAESSAWSDRIPNQLKSIVGDVNKFFDEDLQRDVPTGTTPARREFSYPRQFIATSPHEQILQRFRQARKPIETSEDDSILSKPYSTVNDEIVINSDNKPSKKIHKAMQVYFQQAEEYKEFIQKQIAEYDIGKRHLANMMGEDPENFSEADINRSIKYLFPSGLYEPEARPMIIHPSKLYASRKEAEFDESGRPHHFLYYTTKPNYYNILHKIAENMIKLNELEDKTAQIKKYSTIEDKFDVAKTEWLNKTELEEKLLEELSDAQYEYFLTSIERVLQHPFSNHASSLLNECRKQITAISTTMKLPLVQFDENNRPYILLKNCARKCARGDVKVIGNGTGNITINGEDISYFTDIQCREQVIFPLIFSDMSDKVDVEVTVSGGGPTGQAGLIRWGIAKALRNFVSKEMIEKMRIAGLLTHDMRRRERKKFGQEGARRKFTWKKR

Target ORF information:

RefSeq Version	XM_012279660.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata bipolar kinesin KRP-130 (LOC100882136), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012279660.1

ORF Insert Sequence:

ATGTTGTTGT TAATCCATTA TTGGAACAAG TTTTGGCTGG ATATAATTGT ACAGTGTTTG 
CATATGGTCA AACTGGTACA GGAAAAACAT TTACTATGGA AGGAAATAAT AGTGATCCAA 
CTTTGCATTG GCAAAATATT ATATGAAGAT GCATCAAAAA AAGGTACTAT AACTATTCAT 
GGTTTAGAAG AAGTAAGAGT ACGTAATAAA AGTGAAGTTT ATAAAATTCT GGAAAAGGGA 
TCAGAGAGGA GAAAAACTGC TGCAACGCTG TTAAACAGTC AATCTAGTCG TTCTCATACT 
GTATTTTCGA TTACTGTACA CATGAAAGAG AATACCATTG ACGGAGAAGA AGTTTTAAAA 
ACGGGAAAGT TAAATTTGGT AGATTTAGCA GGAAGCGAAA GTATCGGGAG ATCTGGCGCT 
ATTGAAAAGA GGGCAAGAGA AGCTGGTAGT ATTAATCAGT CCTTATTAAC CCTTGGTAGA 
GTTATAACAG CTCTTGTTGA AAGAGCACCT CATGTACCTT ATAGAGAATC CAAACTCACT 
AGGTTGCTAC AAGAGTCACT GGGTGGTCGA ACAAAAACCT CTATTATCGC TACAATATCT 
CCTGCTGCTA TTAATCTCGA AGAAACGCTA TCAACATTAG ATTATGCACA TCGAGCGAGA 
AACATTACTA ATCGTCCAGA AATAAATCAA AAACTTTCAA AAAAAGAATT CCTTAAACAG 
TATAGAGTTG AAATCGAAAG ATTACGAAAA GATTTAGAAG CAACGCGTGA GGAGAATGGA 
GTTTGTGTCG CTAATGAGAA TTATCAAGAA ATGCAGACAA TGATCGCTCA ATTAAATAAA 
GATATAAAAG AGAAAATAAA TCACATAAAA GCGCTTGAAA AAACTGTGCA AGATAAGGAG 
GAAATATACA ATGATCTTGA AGTACAAACT CTTGCTCAAA CGCAAGAATT CTACAAAATT 
AAAAATAAAT TAGATAATGT TACGAGTGCC CTTAAATCTA CAGATGAAGG GTTAAAAATG 
ACGATTGAGG AACGAGATAC ACAGAAATTC TTACTAGAAA AATATGTTAA TACAGAACAA 
TCTCTATTAA ATCAAGCTCA AAATTTATTA AATATTGCAG ATACAGCAAC GTCAGATTTG 
TATAAGTTAC ATGATAAGAT TGAACGTCAA ACGCAAACTG AACAAACGTT TAAAGCCCTT 
AGTGAACAGT TCAGAAACAA TGTTTCTTAT TGTGTACAAG GAATGGAGAA GGATGTTATT 
GCGTGTGTAA AAAGATTAAT AAATTGTTTT ACATCTATAA AGAATGATAT TGATCTGTAT 
GCTAACAATA CATGTGAAAG TGTAAACACA GCAATACAAC AAATTGCAAT GGATCTTATT 
AAACAACATT TATCCAATAT TGATAATTTA AAGAAGAGTA TAAACGAATT TTATTCAAAT 
GACCAAAAGT GCATAAAAGA TAAATCAACT GTAATTCCAG TGAGTACAGT AGAATGTAAA 
CATGAATTAT TAAATAAAAT TTCTATAACA TTGGTAAATG ATATTCAAAA TATAATGAGA 
AATGAAATTG TTAAAAATTT GCAAATAATA AGGAATAATG TGACCCAAAA ATTAAAGCGA 
ACTTCAATTC TTATGAAAGA AATGATAGAT TCTGCATGTT ATGTTCAACT AGAAGCTTAC 
AATGATATAA ATAAAAACAT TCAAATTATT GCAAATGATG TAGAAATGCT CGATAAAAGG 
CAACAGTTTA CAGAAAAACA AAAATTATTC GCAAAGATGA TGCAAGATGC AGTTTCTCAA 
TTCAACCATT TGTATAAGAG TCAAGAGGAG CACTATTCTT CTGTAACTGA CAAATGTAAT 
TATGTCAGCA AAATTTGCAA TGATATCACT GATTATTCAA CAAAAAGTAT CGCGTTGCAA 
AACGATCTAA AAGATCAAAT ACAACGTAAC TTACAAAAAA TTGAAACAAA TGTCATTCTT 
AAAACAGAAG AGAGTGAAGA GTTAGCAGAT AAAACAGCAA AGCAAGGAAA AATTCTAATT 
AACGCATTAG AATTAAATAT AAACGAAAGC TGTAACATAT TGAAACAATA TAAAGACCTT 
ACTGAGAATA GCGTTAAAGA GGTACAACAG AAATTTGATG TGGATAGAAA TAAAGCTCTT 
TTATTGGTTA ATAATGCTCA GGAAATAATT ACGAATGTAA GTAAAGATGC AGGATTAATG 
AAAGGCCAAC AAATTAAAAT AACTGAAATC TCTAACAAAA TAATTGGTAA ATTAGAGGAT 
CAAAGTGCAG AATCAAGTGC ATGGAGTGAT AGAATTCCTA ATCAGTTAAA ATCAATAGTG 
GGCGATGTTA ATAAATTTTT TGATGAAGAT TTACAACGTG ATGTTCCAAC AGGAACAACT 
CCTGCTAGGA GAGAATTTTC TTATCCTCGT CAATTTATTG CAACATCTCC TCATGAACAA 
ATTCTTCAAA GATTCAGACA AGCTAGGAAA CCTATTGAAA CGTCGGAAGA TGATAGTATC 
CTGTCTAAAC CATATTCTAC AGTTAATGAT GAAATAGTTA TTAATTCTGA TAACAAGCCA 
AGTAAAAAAA TACACAAAGC TATGCAAGTT TATTTCCAAC AAGCTGAGGA ATATAAGGAA 
TTTATACAAA AACAAATAGC AGAATATGAT ATTGGAAAAC GACATCTAGC TAATATGATG 
GGCGAAGATC CTGAGAATTT TTCTGAGGCA GATATTAATA GATCAATAAA ATATTTATTT 
CCATCTGGTT TATATGAACC AGAAGCTCGT CCTATGATAA TTCATCCTAG TAAATTATAT 
GCAAGCAGAA AAGAAGCTGA ATTTGATGAA AGTGGCAGAC CACATCACTT CCTATATTAT 
ACTACAAAAC CTAACTATTA TAACATACTT CATAAAATTG CAGAAAATAT GATTAAATTA 
AATGAGCTGG AGGATAAGAC CGCACAAATA AAGAAATACA GTACCATAGA GGATAAATTT 
GATGTTGCAA AAACAGAATG GCTAAACAAA ACTGAATTAG AGGAGAAATT ATTGGAAGAA 
CTCTCTGATG CACAATATGA ATACTTTCTA ACAAGTATAG AAAGAGTGCT TCAACATCCC 
TTTTCAAATC ATGCATCTTC TTTGCTCAAC GAATGTAGAA AACAGATAAC TGCGATTTCT 
ACTACCATGA AGCTCCCACT AGTACAATTT GATGAGAATA ATAGACCTTA TATTTTATTA 
AAGAATTGTG CAAGGAAATG CGCACGAGGC GACGTTAAAG TGATAGGTAA TGGAACTGGA 
AACATTACTA TTAATGGAGA AGACATTTCA TATTTTACGG ATATACAGTG TCGTGAGCAG 
GTTATATTTC CATTAATATT TTCGGATATG AGCGACAAAG TTGATGTTGA AGTAACCGTT 
TCTGGTGGGG GACCAACTGG ACAAGCAGGC CTCATTCGTT GGGGTATTGC AAAGGCGCTT 
CGAAATTTTG TCAGTAAGGA AATGATAGAA AAAATGAGAA TTGCTGGACT TCTAACACAC 
GATATGAGGC GCCGCGAAAG GAAGAAGTTT GGCCAGGAAG GAGCTCGAAG AAAGTTCACA 
TGGAAGAAAC GTTAG