LOC100881299 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100881299
Entrez Gene ID	100881299
Full Name	laccase-1-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003699519.1	XP_003699567.1	laccase-1-like

Pathways from BioSystems
Gene Ontology

The following LOC100881299 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100881299 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe97122	XM_003699519.1 Latest version!	Megachile rotundata laccase-1-like (LOC100881299), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100881299 ( XM_003699519.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003699567.1 Megachile rotundata laccase-1-like (LOC100881299), mRNA.

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CloneID	OMe97122
Clone ID Related Accession (Same CDS sequence)	XM_003699519.1
Accession Version	XM_003699519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	laccase-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797070.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGAACTGCA ACCAAGACGC ACTTTTATCT TCTCTGCTCA TTCTTGCTGT TATAGTTAAA 
TGTGGATCAT GTGAGCAAAC TCCAGTCGTT GGATATGTTC CTGGAGTTAG TGGCATGTCT 
ACTGTAAATG TGAAAAATTG TGAGATACCT GAAACCTTGA CGGATGTACC TCTATCAAAT 
GCCCTCGAGT GTAATCGTCC CTGTCATGTT GGGCAGAAAC CAAAAACTTG TTATTACAAA 
TTCGTGCTTG AACGTTATCC CGTCAGCGGG CAAGCATGCA ATTTCTGTAC CCCTAATGTC 
ACAAATACAT TGTGTCCCAA TTGTCAATGC ATAACTAGCG ACGGAGTGGA GCGTATGGCT 
TTAGTTGTGA ACCGAATGCT ACCAGGACCG AGTATACAAG TTTGTCTAAA TGATCGTGTG 
GTGGTCGACG TTTTGAACAA AATTCCAGAA GATGGAGTGA CGATTCATTG GCATGGGGTG 
TATCAGAATG GATCTCAATA TTATGATGGT GTACCTGCAT TAACCCAATG CCCTATTACG 
TCTGGCACAA CTTTTAGATA TCAGTTTCCC GCGAAGAATG CTGGCACCCA CTTCTGGCAT 
GCTCATACGG GATTAAACAA GATGGACGGC GTTTTCGGTA GCCTTGTTAT AAGAGATCCA 
ATAGAACAAG ATCCTTATTC GAATCAATAT GATTTTGATT TAGCAAATCA CGTGATAGTC 
ATTAGTGACT GGTTTCACGA AGAATCGTCA GAAAGATTCC CAGGCCGCAA TAATATAAGT 
GGTGTAACTG GTCAAACTCC TGATACGTTT CTGATCAATG GAAAAGGAAA ATACTTGACC 
GTCAATAATG ACACAGGGAA GGGAGCTTTC GAAGTAATAA CTGTAGAAGC CAATAAACGT 
TATCGTTTCC GTTTGGTCAA TGCGTTTTGT GCCAACTGTG CTGGCCAATT GATGATTGAA 
GGCCACAACT TGACCGCTAT CGCGACGGAT GGACAATATA TTGAACCCGT CGCTGTGGAT 
GCTATTGTCT CATTAGCAGG CGAACGATAT GATTTTATCA TAAATACTAA TCAAGAGCCT 
GGTACTTATT GGATACAACT TCGTGGATTA ACTGAATGTA CAACACCAAA TATTCATCAG 
TTAGCCTTAT TGCAGTATGT AAATGCATCT ACAGAACCAA AAACTCCACA GCCTTCTTAC 
GGTGAAATCC CTTCGAATGT AATTTTAAAT CCAGTGGACA TTCCCTGCAA CAAAACGAAT 
TCGCATGTGA TATGTATAAG CAACTTAACG CATGCCTCGG ATATTGAATC TGACGTTGGG 
CAAGGCGAAG CAGATTTAAA GTTTTACATG CCTATTGACT TTCGTGCGCC CTCACCCGAC 
ACCTTTTATC AGCCTAACAC GTACGAAGAT TTTTTGATTC CAACGCCAGA TATCATTGTT 
TTGGGAATTA TGGACAACAT TCAATTTGTT TTCCCACCAT CACCACCACT GTCACAGTAT 
GAAGATCTAC CTCCGAATCA GTTTTGCGAT TCTGAAAATT TGCCAGATGA TTGCAGCGGC 
AATTGTAGTT GCACGCACGT GTTGAAAATA CCGTTGGACG CTGTAGTGGA AGTGATATTA 
ATTGACGTAT CCGGTATACC AGAGCTGTTT CATCCTTTCC ATTTGCATGG TTATGCATTT 
CGTGTCCTAA GTATGGGCCA ACCATTAGGT CCTCCCACAA ATGAAAGTTT AATAAGTCTA 
GATTACGTGA AGGAGCTGGA TAGTGAGACT AATTTAAACA GAAATTTTGA TAATCCTCCA 
GGAAAAGATA CAATTGTTCT TCCAAATAAC GGATACGCGG TAATCAGATT CCGTGCGAAT 
AACCCAGGGT TTTGGTTGTT CCACTGCCAT TTTATTTTTC ATCAAGATGC CGGCATGGAG 
CTCGTTTTTC AAGTTGGGCA CCAAAGTGAT GTACCACCGG TGCCAAAGAA TTTTCCACGA 
TGTGGCAATT TTATGCCGAA TATTAAACGT GTAAATCAAA ATTGTGACGC AAAATACAAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003699567.1
CDS	1..2043
Translation	MNCNQDALLSSLLILAVIVKCGSCEQTPVVGYVPGVSGMSTVNVKNCEIPETLTDVPLSNALECNRPCHVGQKPKTCYYKFVLERYPVSGQACNFCTPNVTNTLCPNCQCITSDGVERMALVVNRMLPGPSIQVCLNDRVVVDVLNKIPEDGVTIHWHGVYQNGSQYYDGVPALTQCPITSGTTFRYQFPAKNAGTHFWHAHTGLNKMDGVFGSLVIRDPIEQDPYSNQYDFDLANHVIVISDWFHEESSERFPGRNNISGVTGQTPDTFLINGKGKYLTVNNDTGKGAFEVITVEANKRYRFRLVNAFCANCAGQLMIEGHNLTAIATDGQYIEPVAVDAIVSLAGERYDFIINTNQEPGTYWIQLRGLTECTTPNIHQLALLQYVNASTEPKTPQPSYGEIPSNVILNPVDIPCNKTNSHVICISNLTHASDIESDVGQGEADLKFYMPIDFRAPSPDTFYQPNTYEDFLIPTPDIIVLGIMDNIQFVFPPSPPLSQYEDLPPNQFCDSENLPDDCSGNCSCTHVLKIPLDAVVEVILIDVSGIPELFHPFHLHGYAFRVLSMGQPLGPPTNESLISLDYVKELDSETNLNRNFDNPPGKDTIVLPNNGYAVIRFRANNPGFWLFHCHFIFHQDAGMELVFQVGHQSDVPPVPKNFPRCGNFMPNIKRVNQNCDAKYN

Target ORF information:

RefSeq Version	XM_003699519.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata laccase-1-like (LOC100881299), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003699519.1

ORF Insert Sequence:

ATGAACTGCA ACCAAGACGC ACTTTTATCT TCTCTGCTCA TTCTTGCTGT TATAGTTAAA 
TGTGGATCAT GTGAGCAAAC TCCAGTCGTT GGATATGTTC CTGGAGTTAG TGGCATGTCT 
ACTGTAAATG TGAAAAATTG TGAGATACCT GAAACCTTGA CGGATGTACC TCTATCAAAT 
GCCCTCGAGT GTAATCGTCC CTGTCATGTT GGGCAGAAAC CAAAAACTTG TTATTACAAA 
TTCGTGCTTG AACGTTATCC CGTCAGCGGG CAAGCATGCA ATTTCTGTAC CCCTAATGTC 
ACAAATACAT TGTGTCCCAA TTGTCAATGC ATAACTAGCG ACGGAGTGGA GCGTATGGCT 
TTAGTTGTGA ACCGAATGCT ACCAGGACCG AGTATACAAG TTTGTCTAAA TGATCGTGTG 
GTGGTCGACG TTTTGAACAA AATTCCAGAA GATGGAGTGA CGATTCATTG GCATGGGGTG 
TATCAGAATG GATCTCAATA TTATGATGGT GTACCTGCAT TAACCCAATG CCCTATTACG 
TCTGGCACAA CTTTTAGATA TCAGTTTCCC GCGAAGAATG CTGGCACCCA CTTCTGGCAT 
GCTCATACGG GATTAAACAA GATGGACGGC GTTTTCGGTA GCCTTGTTAT AAGAGATCCA 
ATAGAACAAG ATCCTTATTC GAATCAATAT GATTTTGATT TAGCAAATCA CGTGATAGTC 
ATTAGTGACT GGTTTCACGA AGAATCGTCA GAAAGATTCC CAGGCCGCAA TAATATAAGT 
GGTGTAACTG GTCAAACTCC TGATACGTTT CTGATCAATG GAAAAGGAAA ATACTTGACC 
GTCAATAATG ACACAGGGAA GGGAGCTTTC GAAGTAATAA CTGTAGAAGC CAATAAACGT 
TATCGTTTCC GTTTGGTCAA TGCGTTTTGT GCCAACTGTG CTGGCCAATT GATGATTGAA 
GGCCACAACT TGACCGCTAT CGCGACGGAT GGACAATATA TTGAACCCGT CGCTGTGGAT 
GCTATTGTCT CATTAGCAGG CGAACGATAT GATTTTATCA TAAATACTAA TCAAGAGCCT 
GGTACTTATT GGATACAACT TCGTGGATTA ACTGAATGTA CAACACCAAA TATTCATCAG 
TTAGCCTTAT TGCAGTATGT AAATGCATCT ACAGAACCAA AAACTCCACA GCCTTCTTAC 
GGTGAAATCC CTTCGAATGT AATTTTAAAT CCAGTGGACA TTCCCTGCAA CAAAACGAAT 
TCGCATGTGA TATGTATAAG CAACTTAACG CATGCCTCGG ATATTGAATC TGACGTTGGG 
CAAGGCGAAG CAGATTTAAA GTTTTACATG CCTATTGACT TTCGTGCGCC CTCACCCGAC 
ACCTTTTATC AGCCTAACAC GTACGAAGAT TTTTTGATTC CAACGCCAGA TATCATTGTT 
TTGGGAATTA TGGACAACAT TCAATTTGTT TTCCCACCAT CACCACCACT GTCACAGTAT 
GAAGATCTAC CTCCGAATCA GTTTTGCGAT TCTGAAAATT TGCCAGATGA TTGCAGCGGC 
AATTGTAGTT GCACGCACGT GTTGAAAATA CCGTTGGACG CTGTAGTGGA AGTGATATTA 
ATTGACGTAT CCGGTATACC AGAGCTGTTT CATCCTTTCC ATTTGCATGG TTATGCATTT 
CGTGTCCTAA GTATGGGCCA ACCATTAGGT CCTCCCACAA ATGAAAGTTT AATAAGTCTA 
GATTACGTGA AGGAGCTGGA TAGTGAGACT AATTTAAACA GAAATTTTGA TAATCCTCCA 
GGAAAAGATA CAATTGTTCT TCCAAATAAC GGATACGCGG TAATCAGATT CCGTGCGAAT 
AACCCAGGGT TTTGGTTGTT CCACTGCCAT TTTATTTTTC ATCAAGATGC CGGCATGGAG 
CTCGTTTTTC AAGTTGGGCA CCAAAGTGAT GTACCACCGG TGCCAAAGAA TTTTCCACGA 
TGTGGCAATT TTATGCCGAA TATTAAACGT GTAAATCAAA ATTGTGACGC AAAATACAAT 
TGA