LOC100881099 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100881099
Entrez Gene ID	100881099
Full Name	translation elongation factor 2
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003705220.2	XP_003705268.1	translation elongation factor 2 isoform X1
XM_012289715.1	XP_012145105.1	translation elongation factor 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100881099 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100881099 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe108484	XM_003705220.2 Latest version!	Megachile rotundata translation elongation factor 2 (LOC100881099), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe108484	XM_012289715.1 Latest version!	Megachile rotundata translation elongation factor 2 (LOC100881099), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100881099 ( XM_003705220.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003705268.1 Megachile rotundata translation elongation factor 2 (LOC100881099), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe108484
Clone ID Related Accession (Same CDS sequence)	XM_003705220.2 , XM_012289715.1
Accession Version	XM_003705220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	translation elongation factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797255.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003705220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACT TTACAGTAGA TGAGATACGT GCCATGATGG ACAAAAAGAA AAACATCAGA 
AATATGTCCG TCATTGCGCA CGTAGATCAT GGAAAGTCAA CTTTGACTGA CTCCCTTGTG 
TCCAAGGCTG GTATCATTGC TGGTGCCAAA GCTGGTGAGA CTCGATTCAC AGATACTCGT 
AAAGATGAGC AAGAACGTTG TATTACAATT AAATCCACGG CTATTTCTAT GTTCTTTGCC 
CTCGACGAGA AAGATTTAGT TTTTATTACA AATCCTGATC AACGTGACAA AGATGAGAAG 
GGTTTCTTGA TTAATCTTAT TGACTCACCT GGACACGTAG ATTTCTCTAG TGAAGTAACA 
GCTGCACTCC GTGTAACTGA TGGAGCTCTC GTAGTTGTTG ATTGTGTATC TGGTGTTTGT 
GTGCAAACGG AAACTGTACT TCGTCAAGCT ATTGCCGAAC GTATAAAGCC TGTACTGTTC 
ATGAACAAGA TGGACAGAGC ATTGTTGGAA CTGCAGCTTG ATAGCGAAGA TCTGTATCAA 
ACTTTCCAAC GTATTGTTGA AAACGTTAAC GTTATTATTG CTACATATTC GGACGATGAT 
GGTCCTATGG GTGAAGTTAG AGTAGACCCC AGTAAAGGAT CGGTCGGTTT CGGTTCTGGT 
CTTCATGGTT GGGCATTCAC GTTAAAACAA TTTTCTGAGA TGTATGCCGA AAAATTCAAA 
ATTGACGTAG TAAAACTAAT GAACAGATTG TGGGGAGAAA CTTTCTTCAA CCCTAAAACT 
AAGAAGTGGA GCAAGCAGAA AGAAGCAGAC AACAAACGTT CTTTCTGTAT GTACGTCTTA 
GATCCAATTT ATAAGGTATT TGATAGCATT ATGAATTACA AAAAAGAAGA AGCAGATAAA 
TTATTGGAAA AATTAGGAAT TGTTCTGAAG CCAGAAGACA AAGACAAGGA TGGTAAAGCT 
CTTCTTAAGG TTGTCATGAG AACATGGTTA CCAGCTGGAG AAGCTTTACT TCAGATGATT 
GCTATTCACT TACCATCTCC TGTTACTGCT CAAAAATACC GTATGGAAAT GTTGTATGAA 
GGTCCACTTG ATGATGAGGC TGCTATTGGC ATTAAGAACT GTGATCCAAA TGGTCCACTT 
ATGATGTATG TTTCAAAAAT GGTACCCACT TCAGATAAAG GTCGTTTCTA TGCTTTCGGT 
CGTGTCTTTT CTGGTAAAGT AAGCACTGGT ATGAAAGCAC GTATTATGGG GCCTAATTTC 
CAACCAGGCA AAAAGGAAGA TCTGTATGAA AAAGCCATTC AGCGTACAAT TTTGATGATG 
GGTCGTTATG TTGAAGCGAT TGAAGATGTG CCTTCTGGTA ATATTTGTGG ACTTGTTGGC 
GTTGATCAAT TCTTAGTAAA GACTGGTACT ATTACTACGT TTAAAGATGC ACATAACATG 
AAAGTCATGA AATTCTCGGT TTCACCTGTT GTACGTGTTG CTGTTGAACC GAAAAATCCA 
GCCGATTTAC CCAAATTGGT CGAAGGTCTC AAACGTTTGG CAAAATCTGA CCCTATGGTG 
CAGTGTATCA TCGAAGAATC AGGAGAGCAC ATTATTGCCG GTGCTGGAGA ACTTCATCTT 
GAAATTTGTT TGAAAGATTT GGAGGAGGAC CATGCTTGCA TACCCATTAA AAAATCGGAT 
CCTGTTGTAT CTTATAGAGA AACGATTTCA GAACAGTCAA ATCAAATGTG TCTTTCAAAA 
TCTCCCAACA AACACAATCG TCTGTTCATG ATGGCGTATC CTATGCCTGA TGGTCTAGCT 
GAAGATATTG ACAGTGGAGA AGTTAATCCA AGAGATGATT TTAAAGTACG TGCTCGTTAC 
TTGAGCGAAA AGTATGATTA CGATGTAACT GAAGCCAGAA AAATTTGGTG CTTCGGACCT 
GACGGAACTG GGCCCAACAT ACTTGTAGAT TGTTCGAAAG GAGTACAATA CCTTAACGAA 
ATTAAAGATT CTGTTGTAGC TGGATTCCAA TGGGCTACGA AAGAAGGTGT TCTTTCGGAA 
GAAAATTTGA GAGGTGTACG TTTCAACATT CATGATGTGA CATTACACGC TGATGCTATT 
CACAGAGGTG GTGGTCAAAT TATTCCTACC ACAAGACGTT GCCTTTATGC TTGTCTCCTC 
ACTGCCTCTC CTCGACTTAT GGAACCAGTT TACTTATGTG AAATTCAGTG CCCAGAAGTA 
GCCGTTGGTG GTATTTATGG TGTGCTTAAC CGAAGAAGAG GTCACGTATT TGAAGAACAA 
CAAGTTGCTG GTACACCCAT GTTTGTAGTT AAAGCATATC TTCCAGTTAA TGAGTCCTTC 
GGTTTTACTG CCGATTTGCG TTCTAATACC GGAGGACAAG CTTTTCCACA ATGTGTATTC 
GATCATTGGC AAATTCTACC TGGTGACCCA ATGGAACCGA ACTCTAGACC TTACCAAGTT 
GTGCAGGAAA CACGTAAAAG GAAGGGATTG AAGGAAGGTC TGCCAGACTT GAACGCATAC 
CTTGATAAAT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003705268.1
CDS	103..2637
Translation	MVNFTVDEIRAMMDKKKNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMFFALDEKDLVFITNPDQRDKDEKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLFMNKMDRALLELQLDSEDLYQTFQRIVENVNVIIATYSDDDGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYAEKFKIDVVKLMNRLWGETFFNPKTKKWSKQKEADNKRSFCMYVLDPIYKVFDSIMNYKKEEADKLLEKLGIVLKPEDKDKDGKALLKVVMRTWLPAGEALLQMIAIHLPSPVTAQKYRMEMLYEGPLDDEAAIGIKNCDPNGPLMMYVSKMVPTSDKGRFYAFGRVFSGKVSTGMKARIMGPNFQPGKKEDLYEKAIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETISEQSNQMCLSKSPNKHNRLFMMAYPMPDGLAEDIDSGEVNPRDDFKVRARYLSEKYDYDVTEARKIWCFGPDGTGPNILVDCSKGVQYLNEIKDSVVAGFQWATKEGVLSEENLRGVRFNIHDVTLHADAIHRGGGQIIPTTRRCLYACLLTASPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEEQQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPMEPNSRPYQVVQETRKRKGLKEGLPDLNAYLDKL

Target ORF information:

RefSeq Version	XM_003705220.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata translation elongation factor 2 (LOC100881099), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003705220.2

ORF Insert Sequence:

ATGGTGAACT TTACAGTAGA TGAGATACGT GCCATGATGG ACAAAAAGAA AAACATCAGA 
AATATGTCCG TCATTGCGCA CGTAGATCAT GGAAAGTCAA CTTTGACTGA CTCCCTTGTG 
TCCAAGGCTG GTATCATTGC TGGTGCCAAA GCTGGTGAGA CTCGATTCAC AGATACTCGT 
AAAGATGAGC AAGAACGTTG TATTACAATT AAATCCACGG CTATTTCTAT GTTCTTTGCC 
CTCGACGAGA AAGATTTAGT TTTTATTACA AATCCTGATC AACGTGACAA AGATGAGAAG 
GGTTTCTTGA TTAATCTTAT TGACTCACCT GGACACGTAG ATTTCTCTAG TGAAGTAACA 
GCTGCACTCC GTGTAACTGA TGGAGCTCTC GTAGTTGTTG ATTGTGTATC TGGTGTTTGT 
GTGCAAACGG AAACTGTACT TCGTCAAGCT ATTGCCGAAC GTATAAAGCC TGTACTGTTC 
ATGAACAAGA TGGACAGAGC ATTGTTGGAA CTGCAGCTTG ATAGCGAAGA TCTGTATCAA 
ACTTTCCAAC GTATTGTTGA AAACGTTAAC GTTATTATTG CTACATATTC GGACGATGAT 
GGTCCTATGG GTGAAGTTAG AGTAGACCCC AGTAAAGGAT CGGTCGGTTT CGGTTCTGGT 
CTTCATGGTT GGGCATTCAC GTTAAAACAA TTTTCTGAGA TGTATGCCGA AAAATTCAAA 
ATTGACGTAG TAAAACTAAT GAACAGATTG TGGGGAGAAA CTTTCTTCAA CCCTAAAACT 
AAGAAGTGGA GCAAGCAGAA AGAAGCAGAC AACAAACGTT CTTTCTGTAT GTACGTCTTA 
GATCCAATTT ATAAGGTATT TGATAGCATT ATGAATTACA AAAAAGAAGA AGCAGATAAA 
TTATTGGAAA AATTAGGAAT TGTTCTGAAG CCAGAAGACA AAGACAAGGA TGGTAAAGCT 
CTTCTTAAGG TTGTCATGAG AACATGGTTA CCAGCTGGAG AAGCTTTACT TCAGATGATT 
GCTATTCACT TACCATCTCC TGTTACTGCT CAAAAATACC GTATGGAAAT GTTGTATGAA 
GGTCCACTTG ATGATGAGGC TGCTATTGGC ATTAAGAACT GTGATCCAAA TGGTCCACTT 
ATGATGTATG TTTCAAAAAT GGTACCCACT TCAGATAAAG GTCGTTTCTA TGCTTTCGGT 
CGTGTCTTTT CTGGTAAAGT AAGCACTGGT ATGAAAGCAC GTATTATGGG GCCTAATTTC 
CAACCAGGCA AAAAGGAAGA TCTGTATGAA AAAGCCATTC AGCGTACAAT TTTGATGATG 
GGTCGTTATG TTGAAGCGAT TGAAGATGTG CCTTCTGGTA ATATTTGTGG ACTTGTTGGC 
GTTGATCAAT TCTTAGTAAA GACTGGTACT ATTACTACGT TTAAAGATGC ACATAACATG 
AAAGTCATGA AATTCTCGGT TTCACCTGTT GTACGTGTTG CTGTTGAACC GAAAAATCCA 
GCCGATTTAC CCAAATTGGT CGAAGGTCTC AAACGTTTGG CAAAATCTGA CCCTATGGTG 
CAGTGTATCA TCGAAGAATC AGGAGAGCAC ATTATTGCCG GTGCTGGAGA ACTTCATCTT 
GAAATTTGTT TGAAAGATTT GGAGGAGGAC CATGCTTGCA TACCCATTAA AAAATCGGAT 
CCTGTTGTAT CTTATAGAGA AACGATTTCA GAACAGTCAA ATCAAATGTG TCTTTCAAAA 
TCTCCCAACA AACACAATCG TCTGTTCATG ATGGCGTATC CTATGCCTGA TGGTCTAGCT 
GAAGATATTG ACAGTGGAGA AGTTAATCCA AGAGATGATT TTAAAGTACG TGCTCGTTAC 
TTGAGCGAAA AGTATGATTA CGATGTAACT GAAGCCAGAA AAATTTGGTG CTTCGGACCT 
GACGGAACTG GGCCCAACAT ACTTGTAGAT TGTTCGAAAG GAGTACAATA CCTTAACGAA 
ATTAAAGATT CTGTTGTAGC TGGATTCCAA TGGGCTACGA AAGAAGGTGT TCTTTCGGAA 
GAAAATTTGA GAGGTGTACG TTTCAACATT CATGATGTGA CATTACACGC TGATGCTATT 
CACAGAGGTG GTGGTCAAAT TATTCCTACC ACAAGACGTT GCCTTTATGC TTGTCTCCTC 
ACTGCCTCTC CTCGACTTAT GGAACCAGTT TACTTATGTG AAATTCAGTG CCCAGAAGTA 
GCCGTTGGTG GTATTTATGG TGTGCTTAAC CGAAGAAGAG GTCACGTATT TGAAGAACAA 
CAAGTTGCTG GTACACCCAT GTTTGTAGTT AAAGCATATC TTCCAGTTAA TGAGTCCTTC 
GGTTTTACTG CCGATTTGCG TTCTAATACC GGAGGACAAG CTTTTCCACA ATGTGTATTC 
GATCATTGGC AAATTCTACC TGGTGACCCA ATGGAACCGA ACTCTAGACC TTACCAAGTT 
GTGCAGGAAA CACGTAAAAG GAAGGGATTG AAGGAAGGTC TGCCAGACTT GAACGCATAC 
CTTGATAAAT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100881099 ( XM_012289715.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145105.1 Megachile rotundata translation elongation factor 2 (LOC100881099), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe108484
Clone ID Related Accession (Same CDS sequence)	XM_003705220.2 , XM_012289715.1
Accession Version	XM_012289715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	translation elongation factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797255.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACT TTACAGTAGA TGAGATACGT GCCATGATGG ACAAAAAGAA AAACATCAGA 
AATATGTCCG TCATTGCGCA CGTAGATCAT GGAAAGTCAA CTTTGACTGA CTCCCTTGTG 
TCCAAGGCTG GTATCATTGC TGGTGCCAAA GCTGGTGAGA CTCGATTCAC AGATACTCGT 
AAAGATGAGC AAGAACGTTG TATTACAATT AAATCCACGG CTATTTCTAT GTTCTTTGCC 
CTCGACGAGA AAGATTTAGT TTTTATTACA AATCCTGATC AACGTGACAA AGATGAGAAG 
GGTTTCTTGA TTAATCTTAT TGACTCACCT GGACACGTAG ATTTCTCTAG TGAAGTAACA 
GCTGCACTCC GTGTAACTGA TGGAGCTCTC GTAGTTGTTG ATTGTGTATC TGGTGTTTGT 
GTGCAAACGG AAACTGTACT TCGTCAAGCT ATTGCCGAAC GTATAAAGCC TGTACTGTTC 
ATGAACAAGA TGGACAGAGC ATTGTTGGAA CTGCAGCTTG ATAGCGAAGA TCTGTATCAA 
ACTTTCCAAC GTATTGTTGA AAACGTTAAC GTTATTATTG CTACATATTC GGACGATGAT 
GGTCCTATGG GTGAAGTTAG AGTAGACCCC AGTAAAGGAT CGGTCGGTTT CGGTTCTGGT 
CTTCATGGTT GGGCATTCAC GTTAAAACAA TTTTCTGAGA TGTATGCCGA AAAATTCAAA 
ATTGACGTAG TAAAACTAAT GAACAGATTG TGGGGAGAAA CTTTCTTCAA CCCTAAAACT 
AAGAAGTGGA GCAAGCAGAA AGAAGCAGAC AACAAACGTT CTTTCTGTAT GTACGTCTTA 
GATCCAATTT ATAAGGTATT TGATAGCATT ATGAATTACA AAAAAGAAGA AGCAGATAAA 
TTATTGGAAA AATTAGGAAT TGTTCTGAAG CCAGAAGACA AAGACAAGGA TGGTAAAGCT 
CTTCTTAAGG TTGTCATGAG AACATGGTTA CCAGCTGGAG AAGCTTTACT TCAGATGATT 
GCTATTCACT TACCATCTCC TGTTACTGCT CAAAAATACC GTATGGAAAT GTTGTATGAA 
GGTCCACTTG ATGATGAGGC TGCTATTGGC ATTAAGAACT GTGATCCAAA TGGTCCACTT 
ATGATGTATG TTTCAAAAAT GGTACCCACT TCAGATAAAG GTCGTTTCTA TGCTTTCGGT 
CGTGTCTTTT CTGGTAAAGT AAGCACTGGT ATGAAAGCAC GTATTATGGG GCCTAATTTC 
CAACCAGGCA AAAAGGAAGA TCTGTATGAA AAAGCCATTC AGCGTACAAT TTTGATGATG 
GGTCGTTATG TTGAAGCGAT TGAAGATGTG CCTTCTGGTA ATATTTGTGG ACTTGTTGGC 
GTTGATCAAT TCTTAGTAAA GACTGGTACT ATTACTACGT TTAAAGATGC ACATAACATG 
AAAGTCATGA AATTCTCGGT TTCACCTGTT GTACGTGTTG CTGTTGAACC GAAAAATCCA 
GCCGATTTAC CCAAATTGGT CGAAGGTCTC AAACGTTTGG CAAAATCTGA CCCTATGGTG 
CAGTGTATCA TCGAAGAATC AGGAGAGCAC ATTATTGCCG GTGCTGGAGA ACTTCATCTT 
GAAATTTGTT TGAAAGATTT GGAGGAGGAC CATGCTTGCA TACCCATTAA AAAATCGGAT 
CCTGTTGTAT CTTATAGAGA AACGATTTCA GAACAGTCAA ATCAAATGTG TCTTTCAAAA 
TCTCCCAACA AACACAATCG TCTGTTCATG ATGGCGTATC CTATGCCTGA TGGTCTAGCT 
GAAGATATTG ACAGTGGAGA AGTTAATCCA AGAGATGATT TTAAAGTACG TGCTCGTTAC 
TTGAGCGAAA AGTATGATTA CGATGTAACT GAAGCCAGAA AAATTTGGTG CTTCGGACCT 
GACGGAACTG GGCCCAACAT ACTTGTAGAT TGTTCGAAAG GAGTACAATA CCTTAACGAA 
ATTAAAGATT CTGTTGTAGC TGGATTCCAA TGGGCTACGA AAGAAGGTGT TCTTTCGGAA 
GAAAATTTGA GAGGTGTACG TTTCAACATT CATGATGTGA CATTACACGC TGATGCTATT 
CACAGAGGTG GTGGTCAAAT TATTCCTACC ACAAGACGTT GCCTTTATGC TTGTCTCCTC 
ACTGCCTCTC CTCGACTTAT GGAACCAGTT TACTTATGTG AAATTCAGTG CCCAGAAGTA 
GCCGTTGGTG GTATTTATGG TGTGCTTAAC CGAAGAAGAG GTCACGTATT TGAAGAACAA 
CAAGTTGCTG GTACACCCAT GTTTGTAGTT AAAGCATATC TTCCAGTTAA TGAGTCCTTC 
GGTTTTACTG CCGATTTGCG TTCTAATACC GGAGGACAAG CTTTTCCACA ATGTGTATTC 
GATCATTGGC AAATTCTACC TGGTGACCCA ATGGAACCGA ACTCTAGACC TTACCAAGTT 
GTGCAGGAAA CACGTAAAAG GAAGGGATTG AAGGAAGGTC TGCCAGACTT GAACGCATAC 
CTTGATAAAT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145105.1
CDS	626..3160
Translation	MVNFTVDEIRAMMDKKKNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMFFALDEKDLVFITNPDQRDKDEKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLFMNKMDRALLELQLDSEDLYQTFQRIVENVNVIIATYSDDDGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYAEKFKIDVVKLMNRLWGETFFNPKTKKWSKQKEADNKRSFCMYVLDPIYKVFDSIMNYKKEEADKLLEKLGIVLKPEDKDKDGKALLKVVMRTWLPAGEALLQMIAIHLPSPVTAQKYRMEMLYEGPLDDEAAIGIKNCDPNGPLMMYVSKMVPTSDKGRFYAFGRVFSGKVSTGMKARIMGPNFQPGKKEDLYEKAIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETISEQSNQMCLSKSPNKHNRLFMMAYPMPDGLAEDIDSGEVNPRDDFKVRARYLSEKYDYDVTEARKIWCFGPDGTGPNILVDCSKGVQYLNEIKDSVVAGFQWATKEGVLSEENLRGVRFNIHDVTLHADAIHRGGGQIIPTTRRCLYACLLTASPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEEQQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPMEPNSRPYQVVQETRKRKGLKEGLPDLNAYLDKL

Target ORF information:

RefSeq Version	XM_012289715.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata translation elongation factor 2 (LOC100881099), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012289715.1

ORF Insert Sequence:

ATGGTGAACT TTACAGTAGA TGAGATACGT GCCATGATGG ACAAAAAGAA AAACATCAGA 
AATATGTCCG TCATTGCGCA CGTAGATCAT GGAAAGTCAA CTTTGACTGA CTCCCTTGTG 
TCCAAGGCTG GTATCATTGC TGGTGCCAAA GCTGGTGAGA CTCGATTCAC AGATACTCGT 
AAAGATGAGC AAGAACGTTG TATTACAATT AAATCCACGG CTATTTCTAT GTTCTTTGCC 
CTCGACGAGA AAGATTTAGT TTTTATTACA AATCCTGATC AACGTGACAA AGATGAGAAG 
GGTTTCTTGA TTAATCTTAT TGACTCACCT GGACACGTAG ATTTCTCTAG TGAAGTAACA 
GCTGCACTCC GTGTAACTGA TGGAGCTCTC GTAGTTGTTG ATTGTGTATC TGGTGTTTGT 
GTGCAAACGG AAACTGTACT TCGTCAAGCT ATTGCCGAAC GTATAAAGCC TGTACTGTTC 
ATGAACAAGA TGGACAGAGC ATTGTTGGAA CTGCAGCTTG ATAGCGAAGA TCTGTATCAA 
ACTTTCCAAC GTATTGTTGA AAACGTTAAC GTTATTATTG CTACATATTC GGACGATGAT 
GGTCCTATGG GTGAAGTTAG AGTAGACCCC AGTAAAGGAT CGGTCGGTTT CGGTTCTGGT 
CTTCATGGTT GGGCATTCAC GTTAAAACAA TTTTCTGAGA TGTATGCCGA AAAATTCAAA 
ATTGACGTAG TAAAACTAAT GAACAGATTG TGGGGAGAAA CTTTCTTCAA CCCTAAAACT 
AAGAAGTGGA GCAAGCAGAA AGAAGCAGAC AACAAACGTT CTTTCTGTAT GTACGTCTTA 
GATCCAATTT ATAAGGTATT TGATAGCATT ATGAATTACA AAAAAGAAGA AGCAGATAAA 
TTATTGGAAA AATTAGGAAT TGTTCTGAAG CCAGAAGACA AAGACAAGGA TGGTAAAGCT 
CTTCTTAAGG TTGTCATGAG AACATGGTTA CCAGCTGGAG AAGCTTTACT TCAGATGATT 
GCTATTCACT TACCATCTCC TGTTACTGCT CAAAAATACC GTATGGAAAT GTTGTATGAA 
GGTCCACTTG ATGATGAGGC TGCTATTGGC ATTAAGAACT GTGATCCAAA TGGTCCACTT 
ATGATGTATG TTTCAAAAAT GGTACCCACT TCAGATAAAG GTCGTTTCTA TGCTTTCGGT 
CGTGTCTTTT CTGGTAAAGT AAGCACTGGT ATGAAAGCAC GTATTATGGG GCCTAATTTC 
CAACCAGGCA AAAAGGAAGA TCTGTATGAA AAAGCCATTC AGCGTACAAT TTTGATGATG 
GGTCGTTATG TTGAAGCGAT TGAAGATGTG CCTTCTGGTA ATATTTGTGG ACTTGTTGGC 
GTTGATCAAT TCTTAGTAAA GACTGGTACT ATTACTACGT TTAAAGATGC ACATAACATG 
AAAGTCATGA AATTCTCGGT TTCACCTGTT GTACGTGTTG CTGTTGAACC GAAAAATCCA 
GCCGATTTAC CCAAATTGGT CGAAGGTCTC AAACGTTTGG CAAAATCTGA CCCTATGGTG 
CAGTGTATCA TCGAAGAATC AGGAGAGCAC ATTATTGCCG GTGCTGGAGA ACTTCATCTT 
GAAATTTGTT TGAAAGATTT GGAGGAGGAC CATGCTTGCA TACCCATTAA AAAATCGGAT 
CCTGTTGTAT CTTATAGAGA AACGATTTCA GAACAGTCAA ATCAAATGTG TCTTTCAAAA 
TCTCCCAACA AACACAATCG TCTGTTCATG ATGGCGTATC CTATGCCTGA TGGTCTAGCT 
GAAGATATTG ACAGTGGAGA AGTTAATCCA AGAGATGATT TTAAAGTACG TGCTCGTTAC 
TTGAGCGAAA AGTATGATTA CGATGTAACT GAAGCCAGAA AAATTTGGTG CTTCGGACCT 
GACGGAACTG GGCCCAACAT ACTTGTAGAT TGTTCGAAAG GAGTACAATA CCTTAACGAA 
ATTAAAGATT CTGTTGTAGC TGGATTCCAA TGGGCTACGA AAGAAGGTGT TCTTTCGGAA 
GAAAATTTGA GAGGTGTACG TTTCAACATT CATGATGTGA CATTACACGC TGATGCTATT 
CACAGAGGTG GTGGTCAAAT TATTCCTACC ACAAGACGTT GCCTTTATGC TTGTCTCCTC 
ACTGCCTCTC CTCGACTTAT GGAACCAGTT TACTTATGTG AAATTCAGTG CCCAGAAGTA 
GCCGTTGGTG GTATTTATGG TGTGCTTAAC CGAAGAAGAG GTCACGTATT TGAAGAACAA 
CAAGTTGCTG GTACACCCAT GTTTGTAGTT AAAGCATATC TTCCAGTTAA TGAGTCCTTC 
GGTTTTACTG CCGATTTGCG TTCTAATACC GGAGGACAAG CTTTTCCACA ATGTGTATTC 
GATCATTGGC AAATTCTACC TGGTGACCCA ATGGAACCGA ACTCTAGACC TTACCAAGTT 
GTGCAGGAAA CACGTAAAAG GAAGGGATTG AAGGAAGGTC TGCCAGACTT GAACGCATAC 
CTTGATAAAT TGTAA