LOC100880268 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100880268
Entrez Gene ID	100880268
Full Name	NAD-dependent protein deacetylase sirtuin-7
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012287821.1	XP_012143211.1	NAD-dependent protein deacetylase sirtuin-7 isoform X2
XM_003704395.2	XP_003704443.1	NAD-dependent protein deacetylase sirtuin-7 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100880268 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100880268 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe106882	XM_012287821.1 Latest version!	Megachile rotundata NAD-dependent protein deacetylase sirtuin-7 (LOC100880268), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OMe106881	XM_003704395.2 Latest version!	Megachile rotundata NAD-dependent protein deacetylase sirtuin-7 (LOC100880268), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100880268 ( XM_012287821.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012143211.1 Megachile rotundata NAD-dependent protein deacetylase sirtuin-7 (LOC100880268), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe106882
Clone ID Related Accession (Same CDS sequence)	XM_012287821.1
Accession Version	XM_012287821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	NAD-dependent protein deacetylase sirtuin-7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797196.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CTGCTAGTGA GAAGTTTCTG TCAAGAAGAC GTTCTGCTGC TTTGAAGGCT 
TTTAAAGTCA AAGATGAACG TGTAGCAACT TTTAAAAAAG TAGCAGCAAT TTTACAAAAA 
TCTGAAACAG ATAGAACCGC AGAAGAAACG GGAATACTTG TGTCTTGTAG TGATGTAGTA 
AAAGAGGTTA ATTTAAGACA AGAGAAAAGA CATAGAGTAA AAGCAAGATT AGAAGAAGTA 
GAAGATGCAC CAGAAATATT AGAAGAAAAG TGCATAAGAT TAGCTGCAGC AATTAGCAGG 
GCAACATCAC TCGCTATTTA TACCGGTGCA GGTATTAGTA CTGCTGCTTC TATCCCAGAC 
TATAGAGGAA CTAATGGTGT GTGGACTCGT TTGCAACAGG GAAAAGATAT CGGAAATCAT 
GATCTTAGTC AAGCAGAACC AACAGTCACC CATATGGCTC TTTATGCTTT ATACAAGGCA 
AGAGTTTTGA AACACATAGT TTCTCAAAAT TGTGATGGAC TCCATTTGCG AAGCGGGATA 
CCCCGCACAC TTTTATCCGA AGTTCATGGC AATATGTATA TAGAAGTTTG CAGAACGTGT 
AAACCTTACA GGGAATATTG GAGATTATTT GACGTTACTG AAAAAACAGC TCGATATTCT 
CACGGGACTG GAAGATTATG TCACAGGTGT AATTCCGTTT TGCACGATTC TATTGTTCAT 
TTTGGTGAAA GAGGCAATCT TCCTTGGCCA ATTAATTGGA ATGGTGCGAC AAGAGCAGCG 
AAGCAAGCAG ACGTTATATT ATGTTTAGGT TCCAGTTTAA AGGTGTTAAA GAAGTATCCA 
TGGTTGTGGC AAATGGATAG ACCAATTCAC AAACGTCCAT CGTTGTACAT TGTAAATTTA 
CAATGGACTC CAAAAGATGA AAATGCAGTT TTAAAAATAA ATGGCAAGTG TGACGAAGTC 
ATGAAAAGAG TTATGGTTCA TCTTGGATTA GAAATACCGC AATATGATCG TACCAAAGAT 
CCAATTTTTT TCCACGCTGT ACGGCTCAGA AGCAATGAAC AACAAACAAC AAGTCAACCC 
TGTCTGGAAG AACCAGCCAA TATGACTCTA AAAGAAATAG ATGAAATTAC TGACAATTTT 
CACGAAGATA TTGCATCGGA AACCAAAGAG GAAGATTGTG CTTCGCCGAT AAAAATATCG 
CAAAACCCAA GCCAACTTGT ACCTTCTGTA TGGAGAACGA GGGGTCACTT ACTTGTCTTT 
ATTACCAACG AGATACCGAT AATACTCGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012143211.1
CDS	19..1311
Translation	MEEAASEKFLSRRRSAALKAFKVKDERVATFKKVAAILQKSETDRTAEETGILVSCSDVVKEVNLRQEKRHRVKARLEEVEDAPEILEEKCIRLAAAISRATSLAIYTGAGISTAASIPDYRGTNGVWTRLQQGKDIGNHDLSQAEPTVTHMALYALYKARVLKHIVSQNCDGLHLRSGIPRTLLSEVHGNMYIEVCRTCKPYREYWRLFDVTEKTARYSHGTGRLCHRCNSVLHDSIVHFGERGNLPWPINWNGATRAAKQADVILCLGSSLKVLKKYPWLWQMDRPIHKRPSLYIVNLQWTPKDENAVLKINGKCDEVMKRVMVHLGLEIPQYDRTKDPIFFHAVRLRSNEQQTTSQPCLEEPANMTLKEIDEITDNFHEDIASETKEEDCASPIKISQNPSQLVPSVWRTRGHLLVFITNEIPIILV

Target ORF information:

RefSeq Version	XM_012287821.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata NAD-dependent protein deacetylase sirtuin-7 (LOC100880268), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012287821.1

ORF Insert Sequence:

ATGGAGGAAG CTGCTAGTGA GAAGTTTCTG TCAAGAAGAC GTTCTGCTGC TTTGAAGGCT 
TTTAAAGTCA AAGATGAACG TGTAGCAACT TTTAAAAAAG TAGCAGCAAT TTTACAAAAA 
TCTGAAACAG ATAGAACCGC AGAAGAAACG GGAATACTTG TGTCTTGTAG TGATGTAGTA 
AAAGAGGTTA ATTTAAGACA AGAGAAAAGA CATAGAGTAA AAGCAAGATT AGAAGAAGTA 
GAAGATGCAC CAGAAATATT AGAAGAAAAG TGCATAAGAT TAGCTGCAGC AATTAGCAGG 
GCAACATCAC TCGCTATTTA TACCGGTGCA GGTATTAGTA CTGCTGCTTC TATCCCAGAC 
TATAGAGGAA CTAATGGTGT GTGGACTCGT TTGCAACAGG GAAAAGATAT CGGAAATCAT 
GATCTTAGTC AAGCAGAACC AACAGTCACC CATATGGCTC TTTATGCTTT ATACAAGGCA 
AGAGTTTTGA AACACATAGT TTCTCAAAAT TGTGATGGAC TCCATTTGCG AAGCGGGATA 
CCCCGCACAC TTTTATCCGA AGTTCATGGC AATATGTATA TAGAAGTTTG CAGAACGTGT 
AAACCTTACA GGGAATATTG GAGATTATTT GACGTTACTG AAAAAACAGC TCGATATTCT 
CACGGGACTG GAAGATTATG TCACAGGTGT AATTCCGTTT TGCACGATTC TATTGTTCAT 
TTTGGTGAAA GAGGCAATCT TCCTTGGCCA ATTAATTGGA ATGGTGCGAC AAGAGCAGCG 
AAGCAAGCAG ACGTTATATT ATGTTTAGGT TCCAGTTTAA AGGTGTTAAA GAAGTATCCA 
TGGTTGTGGC AAATGGATAG ACCAATTCAC AAACGTCCAT CGTTGTACAT TGTAAATTTA 
CAATGGACTC CAAAAGATGA AAATGCAGTT TTAAAAATAA ATGGCAAGTG TGACGAAGTC 
ATGAAAAGAG TTATGGTTCA TCTTGGATTA GAAATACCGC AATATGATCG TACCAAAGAT 
CCAATTTTTT TCCACGCTGT ACGGCTCAGA AGCAATGAAC AACAAACAAC AAGTCAACCC 
TGTCTGGAAG AACCAGCCAA TATGACTCTA AAAGAAATAG ATGAAATTAC TGACAATTTT 
CACGAAGATA TTGCATCGGA AACCAAAGAG GAAGATTGTG CTTCGCCGAT AAAAATATCG 
CAAAACCCAA GCCAACTTGT ACCTTCTGTA TGGAGAACGA GGGGTCACTT ACTTGTCTTT 
ATTACCAACG AGATACCGAT AATACTCGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880268 ( XM_003704395.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003704443.1 Megachile rotundata NAD-dependent protein deacetylase sirtuin-7 (LOC100880268), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe106881
Clone ID Related Accession (Same CDS sequence)	XM_003704395.2
Accession Version	XM_003704395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	NAD-dependent protein deacetylase sirtuin-7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797196.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003704395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CTGCTAGTGA GAAGTTTCTG TCAAGAAGAC GTTCTGCTGC TTTGAAGGCT 
TTTAAAGTCA AAGATGAACG TGTAGCAACT TTTAAAAAAG TAGCAGCAAT TTTACAAAAA 
TCTGAAACAG ATAGAACCGC AGAAGAAACG GGAATACTTG TGTCTTGTAG TGATGTAGTA 
AAAGAGGTTA ATTTAAGACA AGAGAAAAGA CATAGAGTAA AAGCAAGATT AGAAGAAGTA 
GAAGATGCAC CAGAAATATT AGAAGAAAAG TGCATAAGAT TAGCTGCAGC AATTAGCAGG 
GCAACATCAC TCGCTATTTA TACCGGTGCA GGTATTAGTA CTGCTGCTTC TATCCCAGAC 
TATAGAGGAA CTAATGGTGT GTGGACTCGT TTGCAACAGG GAAAAGATAT CGGAAATCAT 
GATCTTAGTC AAGCAGAACC AACAGTCACC CATATGGCTC TTTATGCTTT ATACAAGGCA 
AGAGTTTTGA AACACATAGT TTCTCAAAAT TGTGATGGAC TCCATTTGCG AAGCGGGATA 
CCCCGCACAC TTTTATCCGA AGTTCATGGC AATATGTATA TAGAAGTTTG CAGAACGTGT 
AAACCTTACA GGGAATATTG GAGATTATTT GACGTTACTG AAAAAACAGC TCGATATTCT 
CACGGGACTG GAAGATTATG TCACAGGTGT AATTCCGTTT TGCACGATTC TATTGTTCAT 
TTTGGTGAAA GAGGCAATCT TCCTTGGCCA ATTAATTGGA ATGGTGCGAC AAGAGCAGCG 
AAGCAAGCAG ACGTTATATT ATGTTTAGGT TCCAGTTTAA AGGTGTTAAA GAAGTATCCA 
TGGTTGTGGC AAATGGATAG ACCAATTCAC AAACGTCCAT CGTTGTACAT TGTAAATTTA 
CAATGGACTC CAAAAGATGA AAATGCAGTT TTAAAAATAA ATGGCAAGTG TGACGAAGTC 
ATGAAAAGAG TTATGGTTCA TCTTGGATTA GAAATACCGC AATATGATCG TACCAAAGAT 
CCAATTTTTT TCCACGCTGT ACGGCTCAGA AGCAATGAAC AACAAACAAC AAGTCAACCC 
TGTCTGGAAG AACCAGCCAA TATGACTCTA AAAGAAATAG ATGAAATTAC TGACAATTTT 
CACGAAGATA TTGCATCGGA AACCAAAGAG GAAGATTGTG CTTCGCCGAT AAAAGTAACT 
GAACCAATGT CTACGTATTC AGCGCCATTT TTTACCGCAT TACCATTTTT GTCCATGGGT 
TTACCATTTC CACCAATGTA CATGTGTCCT CAATTAACAC CATTTTTTTA TTATCCATTT 
GTACAAGTAC CCAATATGAC TTCAGATATC GCAAAACCCA AGCCAACTTG TACCTTCTGT 
ATGGAGAACG AGGGGTCACT TACTTGTCTT TATTACCAAC GAGATACCGA TAATACTCGT 
TTAATAGGAG CTGAGAGCAA GCAAATGAAA GATGCAAAAA CATTGGTAAT TCGTGCAGAT 
CCTCCAATAG CGCCTAAAAA TCCCGGGTGG TTTGGTAAAG GATACCGCAA AGGCATGAAG 
AAAAAACGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003704443.1
CDS	18..1589
Translation	MEEAASEKFLSRRRSAALKAFKVKDERVATFKKVAAILQKSETDRTAEETGILVSCSDVVKEVNLRQEKRHRVKARLEEVEDAPEILEEKCIRLAAAISRATSLAIYTGAGISTAASIPDYRGTNGVWTRLQQGKDIGNHDLSQAEPTVTHMALYALYKARVLKHIVSQNCDGLHLRSGIPRTLLSEVHGNMYIEVCRTCKPYREYWRLFDVTEKTARYSHGTGRLCHRCNSVLHDSIVHFGERGNLPWPINWNGATRAAKQADVILCLGSSLKVLKKYPWLWQMDRPIHKRPSLYIVNLQWTPKDENAVLKINGKCDEVMKRVMVHLGLEIPQYDRTKDPIFFHAVRLRSNEQQTTSQPCLEEPANMTLKEIDEITDNFHEDIASETKEEDCASPIKVTEPMSTYSAPFFTALPFLSMGLPFPPMYMCPQLTPFFYYPFVQVPNMTSDIAKPKPTCTFCMENEGSLTCLYYQRDTDNTRLIGAESKQMKDAKTLVIRADPPIAPKNPGWFGKGYRKGMKKKR

Target ORF information:

RefSeq Version	XM_003704395.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata NAD-dependent protein deacetylase sirtuin-7 (LOC100880268), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003704395.2

ORF Insert Sequence:

ATGGAGGAAG CTGCTAGTGA GAAGTTTCTG TCAAGAAGAC GTTCTGCTGC TTTGAAGGCT 
TTTAAAGTCA AAGATGAACG TGTAGCAACT TTTAAAAAAG TAGCAGCAAT TTTACAAAAA 
TCTGAAACAG ATAGAACCGC AGAAGAAACG GGAATACTTG TGTCTTGTAG TGATGTAGTA 
AAAGAGGTTA ATTTAAGACA AGAGAAAAGA CATAGAGTAA AAGCAAGATT AGAAGAAGTA 
GAAGATGCAC CAGAAATATT AGAAGAAAAG TGCATAAGAT TAGCTGCAGC AATTAGCAGG 
GCAACATCAC TCGCTATTTA TACCGGTGCA GGTATTAGTA CTGCTGCTTC TATCCCAGAC 
TATAGAGGAA CTAATGGTGT GTGGACTCGT TTGCAACAGG GAAAAGATAT CGGAAATCAT 
GATCTTAGTC AAGCAGAACC AACAGTCACC CATATGGCTC TTTATGCTTT ATACAAGGCA 
AGAGTTTTGA AACACATAGT TTCTCAAAAT TGTGATGGAC TCCATTTGCG AAGCGGGATA 
CCCCGCACAC TTTTATCCGA AGTTCATGGC AATATGTATA TAGAAGTTTG CAGAACGTGT 
AAACCTTACA GGGAATATTG GAGATTATTT GACGTTACTG AAAAAACAGC TCGATATTCT 
CACGGGACTG GAAGATTATG TCACAGGTGT AATTCCGTTT TGCACGATTC TATTGTTCAT 
TTTGGTGAAA GAGGCAATCT TCCTTGGCCA ATTAATTGGA ATGGTGCGAC AAGAGCAGCG 
AAGCAAGCAG ACGTTATATT ATGTTTAGGT TCCAGTTTAA AGGTGTTAAA GAAGTATCCA 
TGGTTGTGGC AAATGGATAG ACCAATTCAC AAACGTCCAT CGTTGTACAT TGTAAATTTA 
CAATGGACTC CAAAAGATGA AAATGCAGTT TTAAAAATAA ATGGCAAGTG TGACGAAGTC 
ATGAAAAGAG TTATGGTTCA TCTTGGATTA GAAATACCGC AATATGATCG TACCAAAGAT 
CCAATTTTTT TCCACGCTGT ACGGCTCAGA AGCAATGAAC AACAAACAAC AAGTCAACCC 
TGTCTGGAAG AACCAGCCAA TATGACTCTA AAAGAAATAG ATGAAATTAC TGACAATTTT 
CACGAAGATA TTGCATCGGA AACCAAAGAG GAAGATTGTG CTTCGCCGAT AAAAGTAACT 
GAACCAATGT CTACGTATTC AGCGCCATTT TTTACCGCAT TACCATTTTT GTCCATGGGT 
TTACCATTTC CACCAATGTA CATGTGTCCT CAATTAACAC CATTTTTTTA TTATCCATTT 
GTACAAGTAC CCAATATGAC TTCAGATATC GCAAAACCCA AGCCAACTTG TACCTTCTGT 
ATGGAGAACG AGGGGTCACT TACTTGTCTT TATTACCAAC GAGATACCGA TAATACTCGT 
TTAATAGGAG CTGAGAGCAA GCAAATGAAA GATGCAAAAA CATTGGTAAT TCGTGCAGAT 
CCTCCAATAG CGCCTAAAAA TCCCGGGTGG TTTGGTAAAG GATACCGCAA AGGCATGAAG 
AAAAAACGGT AG