LOC100879871 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100879871
Entrez Gene ID	100879871
Full Name	peptide chain release factor 1-like, mitochondrial
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012294833.1	XP_012150223.1	peptide chain release factor 1-like, mitochondrial isoform X2
XM_003707394.2	XP_003707442.2	peptide chain release factor 1-like, mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100879871 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100879871 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe113031	XM_003707394.2 Latest version!	Megachile rotundata peptide chain release factor 1-like, mitochondrial (LOC100879871), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OMe113032	XM_012294833.1 Latest version!	Megachile rotundata peptide chain release factor 1-like, mitochondrial (LOC100879871), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100879871 ( XM_003707394.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003707442.2 Megachile rotundata peptide chain release factor 1-like, mitochondrial (LOC100879871), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe113031
Clone ID Related Accession (Same CDS sequence)	XM_003707394.2
Accession Version	XM_003707394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	peptide chain release factor 1-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTTC TTATAAGAGG ATGCACATTT AGTGCTTGTC GTTATTATGG GAACATAATA 
AATAAGGTCA AACCATCTCC ATTGTGCCCG TTGTTACTTA ACCTCAATAA AAAAGATCCA 
TCTATTTGTA AATTCTTTAG TGCAGAAAAA TATTCAATTA CTCTCGATGA AAATATTAAA 
AAATACTTGA ATCGCCTTAC GGATGTGTAT CAAAATAATG AGAAACAAAT GAATGAAAGC 
ATTAACGAGA TTCTTAAATT AGAAACAATA CCAGAATTAT TGGACAAAAA AATTAAAATA 
ATAGAAAATA TCAAGAGTTT AACTAATCTT GCTAGTAACG ACGAAGAAAT GAAGAAACTT 
GTGAAAGAAG AACAGTTAAT GTATCAACAA GAATTGGTGG AAGTTGATAA ACATATATTA 
GATGTAATTT TACAAACTCT AGGCAAGGAA TATTTTGACC AAATTATTAT GGAGATAGTG 
GCGGGTGCAG GAGGTCAAGA AGCTATGTTG TTTGCTAAGG ATTTATTTAA TATGTATATA 
AATTATTTAA ATTATTTAGA ATATGAATAT GAAGTATTAG AAATGTCGGT TAGTCCTATT 
AGCGGATTGA GAAAAGGTGT GATATTAATA TCAGATAGCA GAGCTTTCGA GAAATTGAAA 
TACGAAGGTG GTATCCATAG AGTACAAAGA GTTCCAGTGA CAGAAAAAAG TGGTCGAATG 
CATACCAGTA CAGTTTTCGT AACAATCTTT CCAGAACCTA GGGATGTAGA TATTAAATTA 
GAAGAAAAAG ATCTAAAAAT AGAATCCAAA AGAGCATCTG GAGCCGGAGG CCAACATGTG 
AATACTACAG ATTCTGCTAT TAGGATAACT CACTTACCAA CAGGTATATC TGTTACTAAC 
CAAACAGATA GATCCCAATT AAAAAACAAA AAGGTAGCAT TAATGAAGTT GAAAAGTATA 
TTATATCAAA TTGAAATGGA CAAGCAAGTA TCGTTCATTA CTGCACTACG TAAAAAGCAA 
ATGACGAAAC TGGAGAAGAT CAGAACGTAT AATTTTAATC AAGATCGCAT AACTGATCAT 
AGAATACTGG ATGGTACGAT GCATAATTTA AAAGGCTTTC TGAAGGACGG ACTGCCTCTG 
GTGGAACTAC AGGATAGACT CTACAAAGAT ATGCAGCACG AGACTTTGTT GGAGATTTTA 
AAGAGTTTGG AGAGTGAATT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003707442.2
CDS	22..1248
Translation	MLFLIRGCTFSACRYYGNIINKVKPSPLCPLLLNLNKKDPSICKFFSAEKYSITLDENIKKYLNRLTDVYQNNEKQMNESINEILKLETIPELLDKKIKIIENIKSLTNLASNDEEMKKLVKEEQLMYQQELVEVDKHILDVILQTLGKEYFDQIIMEIVAGAGGQEAMLFAKDLFNMYINYLNYLEYEYEVLEMSVSPISGLRKGVILISDSRAFEKLKYEGGIHRVQRVPVTEKSGRMHTSTVFVTIFPEPRDVDIKLEEKDLKIESKRASGAGGQHVNTTDSAIRITHLPTGISVTNQTDRSQLKNKKVALMKLKSILYQIEMDKQVSFITALRKKQMTKLEKIRTYNFNQDRITDHRILDGTMHNLKGFLKDGLPLVELQDRLYKDMQHETLLEILKSLESELK

Target ORF information:

RefSeq Version	XM_003707394.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata peptide chain release factor 1-like, mitochondrial (LOC100879871), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707394.2

ORF Insert Sequence:

ATGTTGTTTC TTATAAGAGG ATGCACATTT AGTGCTTGTC GTTATTATGG GAACATAATA 
AATAAGGTCA AACCATCTCC ATTGTGCCCG TTGTTACTTA ACCTCAATAA AAAAGATCCA 
TCTATTTGTA AATTCTTTAG TGCAGAAAAA TATTCAATTA CTCTCGATGA AAATATTAAA 
AAATACTTGA ATCGCCTTAC GGATGTGTAT CAAAATAATG AGAAACAAAT GAATGAAAGC 
ATTAACGAGA TTCTTAAATT AGAAACAATA CCAGAATTAT TGGACAAAAA AATTAAAATA 
ATAGAAAATA TCAAGAGTTT AACTAATCTT GCTAGTAACG ACGAAGAAAT GAAGAAACTT 
GTGAAAGAAG AACAGTTAAT GTATCAACAA GAATTGGTGG AAGTTGATAA ACATATATTA 
GATGTAATTT TACAAACTCT AGGCAAGGAA TATTTTGACC AAATTATTAT GGAGATAGTG 
GCGGGTGCAG GAGGTCAAGA AGCTATGTTG TTTGCTAAGG ATTTATTTAA TATGTATATA 
AATTATTTAA ATTATTTAGA ATATGAATAT GAAGTATTAG AAATGTCGGT TAGTCCTATT 
AGCGGATTGA GAAAAGGTGT GATATTAATA TCAGATAGCA GAGCTTTCGA GAAATTGAAA 
TACGAAGGTG GTATCCATAG AGTACAAAGA GTTCCAGTGA CAGAAAAAAG TGGTCGAATG 
CATACCAGTA CAGTTTTCGT AACAATCTTT CCAGAACCTA GGGATGTAGA TATTAAATTA 
GAAGAAAAAG ATCTAAAAAT AGAATCCAAA AGAGCATCTG GAGCCGGAGG CCAACATGTG 
AATACTACAG ATTCTGCTAT TAGGATAACT CACTTACCAA CAGGTATATC TGTTACTAAC 
CAAACAGATA GATCCCAATT AAAAAACAAA AAGGTAGCAT TAATGAAGTT GAAAAGTATA 
TTATATCAAA TTGAAATGGA CAAGCAAGTA TCGTTCATTA CTGCACTACG TAAAAAGCAA 
ATGACGAAAC TGGAGAAGAT CAGAACGTAT AATTTTAATC AAGATCGCAT AACTGATCAT 
AGAATACTGG ATGGTACGAT GCATAATTTA AAAGGCTTTC TGAAGGACGG ACTGCCTCTG 
GTGGAACTAC AGGATAGACT CTACAAAGAT ATGCAGCACG AGACTTTGTT GGAGATTTTA 
AAGAGTTTGG AGAGTGAATT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100879871 ( XM_012294833.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012150223.1 Megachile rotundata peptide chain release factor 1-like, mitochondrial (LOC100879871), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe113032
Clone ID Related Accession (Same CDS sequence)	XM_012294833.1
Accession Version	XM_012294833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	peptide chain release factor 1-like, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAA GCATTAACGA GATTCTTAAA TTAGAAACAA TACCAGAATT ATTGGACAAA 
AAAATTAAAA TAATAGAAAA TATCAAGAGT TTAACTAATC TTGCTAGTAA CGACGAAGAA 
ATGAAGAAAC TTGTGAAAGA AGAACAGTTA ATGTATCAAC AAGAATTGGT GGAAGTTGAT 
AAACATATAT TAGATGTAAT TTTACAAACT CTAGGCAAGG AATATTTTGA CCAAATTATT 
ATGGAGATAG TGGCGGGTGC AGGAGGTCAA GAAGCTATGT TGTTTGCTAA GGATTTATTT 
AATATGTATA TAAATTATTT AAATTATTTA GAATATGAAT ATGAAGTATT AGAAATGTCG 
GTTAGTCCTA TTAGCGGATT GAGAAAAGGT GTGATATTAA TATCAGATAG CAGAGCTTTC 
GAGAAATTGA AATACGAAGG TGGTATCCAT AGAGTACAAA GAGTTCCAGT GACAGAAAAA 
AGTGGTCGAA TGCATACCAG TACAGTTTTC GTAACAATCT TTCCAGAACC TAGGGATGTA 
GATATTAAAT TAGAAGAAAA AGATCTAAAA ATAGAATCCA AAAGAGCATC TGGAGCCGGA 
GGCCAACATG TGAATACTAC AGATTCTGCT ATTAGGATAA CTCACTTACC AACAGGTATA 
TCTGTTACTA ACCAAACAGA TAGATCCCAA TTAAAAAACA AAAAGGTAGC ATTAATGAAG 
TTGAAAAGTA TATTATATCA AATTGAAATG GACAAGCAAG TATCGTTCAT TACTGCACTA 
CGTAAAAAGC AAATGACGAA ACTGGAGAAG ATCAGAACGT ATAATTTTAA TCAAGATCGC 
ATAACTGATC ATAGAATACT GGATGGTACG ATGCATAATT TAAAAGGCTT TCTGAAGGAC 
GGACTGCCTC TGGTGGAACT ACAGGATAGA CTCTACAAAG ATATGCAGCA CGAGACTTTG 
TTGGAGATTT TAAAGAGTTT GGAGAGTGAA TTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012150223.1
CDS	171..1169
Translation	MNESINEILKLETIPELLDKKIKIIENIKSLTNLASNDEEMKKLVKEEQLMYQQELVEVDKHILDVILQTLGKEYFDQIIMEIVAGAGGQEAMLFAKDLFNMYINYLNYLEYEYEVLEMSVSPISGLRKGVILISDSRAFEKLKYEGGIHRVQRVPVTEKSGRMHTSTVFVTIFPEPRDVDIKLEEKDLKIESKRASGAGGQHVNTTDSAIRITHLPTGISVTNQTDRSQLKNKKVALMKLKSILYQIEMDKQVSFITALRKKQMTKLEKIRTYNFNQDRITDHRILDGTMHNLKGFLKDGLPLVELQDRLYKDMQHETLLEILKSLESELK

Target ORF information:

RefSeq Version	XM_012294833.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata peptide chain release factor 1-like, mitochondrial (LOC100879871), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012294833.1

ORF Insert Sequence:

ATGAATGAAA GCATTAACGA GATTCTTAAA TTAGAAACAA TACCAGAATT ATTGGACAAA 
AAAATTAAAA TAATAGAAAA TATCAAGAGT TTAACTAATC TTGCTAGTAA CGACGAAGAA 
ATGAAGAAAC TTGTGAAAGA AGAACAGTTA ATGTATCAAC AAGAATTGGT GGAAGTTGAT 
AAACATATAT TAGATGTAAT TTTACAAACT CTAGGCAAGG AATATTTTGA CCAAATTATT 
ATGGAGATAG TGGCGGGTGC AGGAGGTCAA GAAGCTATGT TGTTTGCTAA GGATTTATTT 
AATATGTATA TAAATTATTT AAATTATTTA GAATATGAAT ATGAAGTATT AGAAATGTCG 
GTTAGTCCTA TTAGCGGATT GAGAAAAGGT GTGATATTAA TATCAGATAG CAGAGCTTTC 
GAGAAATTGA AATACGAAGG TGGTATCCAT AGAGTACAAA GAGTTCCAGT GACAGAAAAA 
AGTGGTCGAA TGCATACCAG TACAGTTTTC GTAACAATCT TTCCAGAACC TAGGGATGTA 
GATATTAAAT TAGAAGAAAA AGATCTAAAA ATAGAATCCA AAAGAGCATC TGGAGCCGGA 
GGCCAACATG TGAATACTAC AGATTCTGCT ATTAGGATAA CTCACTTACC AACAGGTATA 
TCTGTTACTA ACCAAACAGA TAGATCCCAA TTAAAAAACA AAAAGGTAGC ATTAATGAAG 
TTGAAAAGTA TATTATATCA AATTGAAATG GACAAGCAAG TATCGTTCAT TACTGCACTA 
CGTAAAAAGC AAATGACGAA ACTGGAGAAG ATCAGAACGT ATAATTTTAA TCAAGATCGC 
ATAACTGATC ATAGAATACT GGATGGTACG ATGCATAATT TAAAAGGCTT TCTGAAGGAC 
GGACTGCCTC TGGTGGAACT ACAGGATAGA CTCTACAAAG ATATGCAGCA CGAGACTTTG 
TTGGAGATTT TAAAGAGTTT GGAGAGTGAA TTGAAATAA