LOC100878899 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100878899
Entrez Gene ID	100878899
Full Name	pleckstrin homology domain-containing family M member 2
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012290096.1	XP_012145486.1	LOW QUALITY PROTEIN: pleckstrin homology domain-containing family M member 2

Pathways from BioSystems
Gene Ontology

The following LOC100878899 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100878899 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe108826	XM_012290096.1 Latest version!	Megachile rotundata pleckstrin homology domain-containing family M member 2 (LOC100878899), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100878899 ( XM_012290096.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145486.1 Megachile rotundata pleckstrin homology domain-containing family M member 2 (LOC100878899), mRNA.

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CloneID	OMe108826
Clone ID Related Accession (Same CDS sequence)	XM_012290096.1
Accession Version	XM_012290096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	LOW QUALITY PROTEIN: pleckstrin homology domain-containing family M member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797271.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTGTCAGT GCGACTCCAC CAACGTAGCA TCGGAGGTAC TCCGTCTTGG GTGTCTTCGA 
TTTTCTTCGT CTTTTCGCAC GAAATACGTG TTATCGCCCG ATAAAAATCA TGTAAGATTA 
GAGATCGAGA TGCCAAGTCA GGAAGATATG GACCTTTGTA CCGACAAGGT ACTGTCCAAA 
GGGAGAATAC TTCAGGAATT GACCAAAGCT GTCAAACTGG TTTATGCTCA AAGTTTATAT 
GGTACGGTAG TCTTAGACGG AGATTCATGG TTGGTCTACT GGTTAGATCG AGCTTTGCGT 
CATGGACTAC GTGTAGAGAG GCATGGATAT TGGGGTACTG CTCGTGAACT TAGTCATCAA 
GATACTGTTG TTGTGATACA TGCTCTTCAG AGTGTTTTAA CTTCCATTGG GAAAGGTAGA 
GCATGGCTTT ATCATACTCT CAGCGAAGGT AGCTTCGAAA GTTATCTAGC TTGCTTATTA 
CGAGATACTA AAACATTAAA GAAACAGTAC TTCCCTCATG CACTTGTTCG GGATGTGGAT 
AAAACTAATC AGTTAGTTAA TCTACTTGCT GGATTAGAAA ATGTGTCCTT CACGTTACAG 
TTGGATGTGA CATATTTGGA TACTTGCAAT TACATGCCTC AGAGGCCTAT GAGTGGAACC 
ATATTATCTT TAAATCTCAG ATCATCCAGT GTTTCTTCGA GTTTAGCTTC CCCTGGTGAT 
AGTGGAGTTG CTTTTGATAG TGATATGGAT CTTGCAAATG AAACAGATGC TAGTAGTTAC 
AAAGAGGAAG ATTTTCATCT CGAAGATACT CGTAGATACC GCAATAATAG GTACAAAACA 
ATGATAAACA ATTGCCTGGA GGACAATAAA AACTTCAGTT CCAGTGATTC CAGTGAGGAT 
GGCAAAACAC CTACGCAATC ACCTGGGATA ATGACCAAAT TTCAAACGGT TATGGTGACG 
AAAAGCGACA TAGCGAATCA AAACTTAACT AGAAAACTTG ACGACAATGA GCTTAATTGT 
TCCAGTTTGG ACACCCTGAA GGACTACGAT TTTTACAGTA AAAGTTTAGA CGACTCAAAG 
TTAGATAAGA TAGTCAACAA AAACGATAAC GGAGTCAAAG AGTTGAATAA AAGAAGTACT 
TATTACAGCG ATGGTAGTTA CAGTTTCAAA AGAAACATTG ATCCACGAAA TTCGTACGTG 
ATCAATAATG ATACGAACCT CTTAGAGCTT AGCATGACTA TTTCGGATTG TGAGAACATA 
GAACCCCCGT ATGGTATTCT GAACACCATC AATATGACCG ACGCTAGTAT TCTATCATCT 
TCCAGCTCAG AAGCAATAGT ACAACGTCGA CAACGTAAGA AGAAGCGCGG TGAAAGTAAG 
AAGCGTGTCA GCTTCCACGA AGACATTTTA AAAACTATGA AACTGGATGA CGACGAAAGC 
TTCGCTGATT TTTCAATGAG TTTCTTAACG CCTAATTCAG TACAGAAAAA GGATGCACAA 
AAAGGAAGAT ACAGCTGGTG TGCCCACGGA GATTCGCCTT ATGTCCAGAA GACAGTTAAC 
GCGAGGAACG CACACTCGGA TTACTATTCG TATTCCTCAT CCTCGAGCGT ATTTGATAGC 
GACTGTGACA AGAAAGGATC ACTAAAAACG TGTGAACCTT GTCAGAGTAA TTGTAAATGT 
GCTTGTGGAT TATCAATTTC GGAACGCGGT GTGCCTGAGG GTCAAGAAGA CCCTGGAAAA 
GCTTTAAGAC CAAAAATGGA AATTGAATTG GAGGAAAACG AAGCGCAGGA AGCTTATATC 
GTTGAGAAAC AGTATGGTAA TATTGTTGAA GATCCTCCAT CTAAAGTTCA AATGCCTTGT 
CCGTCTAATT CGAAGAAGTA TACTAGTTCT AGCGATTGGT CAGATGTAGA TAGTACTACT 
GTGTCAGATG TGGATGAACG TAAGAATAGT AGACATTTAG CTTCGCCATC GAAGAAACGT 
AATATGACGT CGATATTAAC TGGAGAAAGT AGTAGCAAAT TATTGGCACC GCCTCTAAGA 
CAAGCTCCAT CTAAAACCTC CTTGTTGAGT AGATTCCTAA AATCCATTAC GGAGAGAAAA 
TTTGAAGTAA AACAGAATAA AAAACCACAA AAGACGAATC CATTGTACAT CAAAGGTGTA 
AAAGCAAGTT ATGATTCATT CAAAGATTTC AATGATAATC TTGATAGAGA AATACAGGAA 
AATGTAGCCG CAGAGAAACA AGAATTCAGC GGCGAGAAGA TCAGTTTGAA GTTACGAGAA 
CTTTTTAAGA AACATATTTA CAGGGACAAA ACTGAAGAGT TATATAAGGT TTACAAAGTT 
AGGAGTTCGT ATATGACAAA CGGGGAAAGC AAACCAATGA TTGCTTTGCT AACGGACAAA 
ACATTATACT TGACAGGTTC AAAGATGAAC CATACTTATA GTAATCAGTT CGTTATTCCT 
TATAACGAGT TAGATGTAAT TATGGTTGGA CCAAATGCAC AAACAATATT ATTATCCAAT 
GCGGATTACG AGATGCAATA CCTCTTTTCT ACTGGATGTT CTCAGAGTAC TTCAGAATTA 
ATTGCACATT TGGAAATAGC CATGAGACGA TCTCCAACAA AACCAAAACT TCCAGCAGTT 
AAAGAATTGA ATTACGAAGA TATGCATATT CTAAGGCATT CGATTCTCTG CGATACGGCT 
GTACATCCAG ATGAGAAAAT TGAACATTAC AACCTTATTT ATATGGAAGA TGAATACATG 
TCACCCCCTA GTACTCCTTG TGGCCCAACA AAAGAAGGTG ACCTTATGTA TAGGCCACAT 
ACTTATCAAC AGCTTCATCC CAACGCTCCG ACTAATCCAT GGGAAGCTGG ATACTTTGTT 
CTTAAAGGTG GTGTCGTTTA CATGTTCACT GATGCAAGTC AACGATTACC CAAACGAGCC 
ATTCCATTAA AAGGTGGTCT CTGTCAGGGT TGTCGAAGAA TTCCTAATTC TCATCGTCCG 
CACACCTTTG AAATACTTTT AAAACCAAAT AAAGCTTTCC AATTCGCTGC TCCGGACGAA 
TACGTTGCTT CGGAGTGGTT GCAAAGTTTC GTTCAAAGTG CTTCTGGATT GTTCGATACT 
TCACGGAAGA GAGAACCTTT ACCATGTAGC CTTATTACAA CCACTAAACA TTTAGTTGCT 
ATGAAAGAAG TATTTCCCGG TAAGCAGCGG GGACAAACAC TTTCTTGTGC CTCTGTAGAA 
GATCTCACAG CCTTCAGAGT ACCTTTAACT CAACAGTCTT GGTGTATATT AGAGTTCGCG 
TGTCGAGAAG TACACGAAAG TAGTGGCGAC TGGGTTATAT ATTTTACAAA TTATACCGAA 
CTGTGTACCT TCAGAGAAAT TTTGGAAACG TTGTGGGCTG ATGCAAGTTT GGGGGAATTT 
CCAATGGTAA CACTTCCACC AGAAGATAAG CTTCATCGGC GTTGCTCAGA TGCCAGTAAA 
GAGCTTGAGC TTGCATGGCG ATATTTATTA TCTTCTGTAA TTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145486.1
CDS	49..3576
Translation	MCQCDSTNVASEVLRLGCLRFSSSFRTKYVLSPDKNHVRLEIEMPSQEDMDLCTDKVLSKGRILQELTKAVKLVYAQSLYGTVVLDGDSWLVYWLDRALRHGLRVERHGYWGTARELSHQDTVVVIHALQSVLTSIGKGRAWLYHTLSEGSFESYLACLLRDTKTLKKQYFPHALVRDVDKTNQLVNLLAGLENVSFTLQLDVTYLDTCNYMPQRPMSGTILSLNLRSSSVSSSLASPGDSGVAFDSDMDLANETDASSYKEEDFHLEDTRRYRNNRYKTMINNCLEDNKNFSSSDSSEDGKTPTQSPGIMTKFQTVMVTKSDIANQNLTRKLDDNELNCSSLDTLKDYDFYSKSLDDSKLDKIVNKNDNGVKELNKRSTYYSDGSYSFKRNIDPRNSYVINNDTNLLELSMTISDCENIEPPYGILNTINMTDASILSSSSSEAIVQRRQRKKKRGESKKRVSFHEDILKTMKLDDDESFADFSMSFLTPNSVQKKDAQKGRYSWCAHGDSPYVQKTVNARNAHSDYYSYSSSSSVFDSDCDKKGSLKTCEPCQSNCKCACGLSISERGVPEGQEDPGKALRPKMEIELEENEAQEAYIVEKQYGNIVEDPPSKVQMPCPSNSKKYTSSSDWSDVDSTTVSDVDERKNSRHLASPSKKRNMTSILTGESSSKLLAPPLRQAPSKTSLLSRFLKSITERKFEVKQNKKPQKTNPLYIKGVKASYDSFKDFNDNLDREIQENVAAEKQEFSGEKISLKLRELFKKHIYRDKTEELYKVYKVRSSYMTNGESKPMIALLTDKTLYLTGSKMNHTYSNQFVIPYNELDVIMVGPNAQTILLSNADYEMQYLFSTGCSQSTSELIAHLEIAMRRSPTKPKLPAVKELNYEDMHILRHSILCDTAVHPDEKIEHYNLIYMEDEYMSPPSTPCGPTKEGDLMYRPHTYQQLHPNAPTNPWEAGYFVLKGGVVYMFTDASQRLPKRAIPLKGGLCQGCRRIPNSHRPHTFEILLKPNKAFQFAAPDEYVASEWLQSFVQSASGLFDTSRKREPLPCSLITTTKHLVAMKEVFPGKQRGQTLSCASVEDLTAFRVPLTQQSWCILEFACREVHESSGDWVIYFTNYTELCTFREILETLWADASLGEFPMVTLPPEDKLHRRCSDASKELELAWRYLLSSVID

Target ORF information:

RefSeq Version	XM_012290096.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pleckstrin homology domain-containing family M member 2 (LOC100878899), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290096.1

ORF Insert Sequence:

ATGTGTCAGT GCGACTCCAC CAACGTAGCA TCGGAGGTAC TCCGTCTTGG GTGTCTTCGA 
TTTTCTTCGT CTTTTCGCAC GAAATACGTG TTATCGCCCG ATAAAAATCA TGTAAGATTA 
GAGATCGAGA TGCCAAGTCA GGAAGATATG GACCTTTGTA CCGACAAGGT ACTGTCCAAA 
GGGAGAATAC TTCAGGAATT GACCAAAGCT GTCAAACTGG TTTATGCTCA AAGTTTATAT 
GGTACGGTAG TCTTAGACGG AGATTCATGG TTGGTCTACT GGTTAGATCG AGCTTTGCGT 
CATGGACTAC GTGTAGAGAG GCATGGATAT TGGGGTACTG CTCGTGAACT TAGTCATCAA 
GATACTGTTG TTGTGATACA TGCTCTTCAG AGTGTTTTAA CTTCCATTGG GAAAGGTAGA 
GCATGGCTTT ATCATACTCT CAGCGAAGGT AGCTTCGAAA GTTATCTAGC TTGCTTATTA 
CGAGATACTA AAACATTAAA GAAACAGTAC TTCCCTCATG CACTTGTTCG GGATGTGGAT 
AAAACTAATC AGTTAGTTAA TCTACTTGCT GGATTAGAAA ATGTGTCCTT CACGTTACAG 
TTGGATGTGA CATATTTGGA TACTTGCAAT TACATGCCTC AGAGGCCTAT GAGTGGAACC 
ATATTATCTT TAAATCTCAG ATCATCCAGT GTTTCTTCGA GTTTAGCTTC CCCTGGTGAT 
AGTGGAGTTG CTTTTGATAG TGATATGGAT CTTGCAAATG AAACAGATGC TAGTAGTTAC 
AAAGAGGAAG ATTTTCATCT CGAAGATACT CGTAGATACC GCAATAATAG GTACAAAACA 
ATGATAAACA ATTGCCTGGA GGACAATAAA AACTTCAGTT CCAGTGATTC CAGTGAGGAT 
GGCAAAACAC CTACGCAATC ACCTGGGATA ATGACCAAAT TTCAAACGGT TATGGTGACG 
AAAAGCGACA TAGCGAATCA AAACTTAACT AGAAAACTTG ACGACAATGA GCTTAATTGT 
TCCAGTTTGG ACACCCTGAA GGACTACGAT TTTTACAGTA AAAGTTTAGA CGACTCAAAG 
TTAGATAAGA TAGTCAACAA AAACGATAAC GGAGTCAAAG AGTTGAATAA AAGAAGTACT 
TATTACAGCG ATGGTAGTTA CAGTTTCAAA AGAAACATTG ATCCACGAAA TTCGTACGTG 
ATCAATAATG ATACGAACCT CTTAGAGCTT AGCATGACTA TTTCGGATTG TGAGAACATA 
GAACCCCCGT ATGGTATTCT GAACACCATC AATATGACCG ACGCTAGTAT TCTATCATCT 
TCCAGCTCAG AAGCAATAGT ACAACGTCGA CAACGTAAGA AGAAGCGCGG TGAAAGTAAG 
AAGCGTGTCA GCTTCCACGA AGACATTTTA AAAACTATGA AACTGGATGA CGACGAAAGC 
TTCGCTGATT TTTCAATGAG TTTCTTAACG CCTAATTCAG TACAGAAAAA GGATGCACAA 
AAAGGAAGAT ACAGCTGGTG TGCCCACGGA GATTCGCCTT ATGTCCAGAA GACAGTTAAC 
GCGAGGAACG CACACTCGGA TTACTATTCG TATTCCTCAT CCTCGAGCGT ATTTGATAGC 
GACTGTGACA AGAAAGGATC ACTAAAAACG TGTGAACCTT GTCAGAGTAA TTGTAAATGT 
GCTTGTGGAT TATCAATTTC GGAACGCGGT GTGCCTGAGG GTCAAGAAGA CCCTGGAAAA 
GCTTTAAGAC CAAAAATGGA AATTGAATTG GAGGAAAACG AAGCGCAGGA AGCTTATATC 
GTTGAGAAAC AGTATGGTAA TATTGTTGAA GATCCTCCAT CTAAAGTTCA AATGCCTTGT 
CCGTCTAATT CGAAGAAGTA TACTAGTTCT AGCGATTGGT CAGATGTAGA TAGTACTACT 
GTGTCAGATG TGGATGAACG TAAGAATAGT AGACATTTAG CTTCGCCATC GAAGAAACGT 
AATATGACGT CGATATTAAC TGGAGAAAGT AGTAGCAAAT TATTGGCACC GCCTCTAAGA 
CAAGCTCCAT CTAAAACCTC CTTGTTGAGT AGATTCCTAA AATCCATTAC GGAGAGAAAA 
TTTGAAGTAA AACAGAATAA AAAACCACAA AAGACGAATC CATTGTACAT CAAAGGTGTA 
AAAGCAAGTT ATGATTCATT CAAAGATTTC AATGATAATC TTGATAGAGA AATACAGGAA 
AATGTAGCCG CAGAGAAACA AGAATTCAGC GGCGAGAAGA TCAGTTTGAA GTTACGAGAA 
CTTTTTAAGA AACATATTTA CAGGGACAAA ACTGAAGAGT TATATAAGGT TTACAAAGTT 
AGGAGTTCGT ATATGACAAA CGGGGAAAGC AAACCAATGA TTGCTTTGCT AACGGACAAA 
ACATTATACT TGACAGGTTC AAAGATGAAC CATACTTATA GTAATCAGTT CGTTATTCCT 
TATAACGAGT TAGATGTAAT TATGGTTGGA CCAAATGCAC AAACAATATT ATTATCCAAT 
GCGGATTACG AGATGCAATA CCTCTTTTCT ACTGGATGTT CTCAGAGTAC TTCAGAATTA 
ATTGCACATT TGGAAATAGC CATGAGACGA TCTCCAACAA AACCAAAACT TCCAGCAGTT 
AAAGAATTGA ATTACGAAGA TATGCATATT CTAAGGCATT CGATTCTCTG CGATACGGCT 
GTACATCCAG ATGAGAAAAT TGAACATTAC AACCTTATTT ATATGGAAGA TGAATACATG 
TCACCCCCTA GTACTCCTTG TGGCCCAACA AAAGAAGGTG ACCTTATGTA TAGGCCACAT 
ACTTATCAAC AGCTTCATCC CAACGCTCCG ACTAATCCAT GGGAAGCTGG ATACTTTGTT 
CTTAAAGGTG GTGTCGTTTA CATGTTCACT GATGCAAGTC AACGATTACC CAAACGAGCC 
ATTCCATTAA AAGGTGGTCT CTGTCAGGGT TGTCGAAGAA TTCCTAATTC TCATCGTCCG 
CACACCTTTG AAATACTTTT AAAACCAAAT AAAGCTTTCC AATTCGCTGC TCCGGACGAA 
TACGTTGCTT CGGAGTGGTT GCAAAGTTTC GTTCAAAGTG CTTCTGGATT GTTCGATACT 
TCACGGAAGA GAGAACCTTT ACCATGTAGC CTTATTACAA CCACTAAACA TTTAGTTGCT 
ATGAAAGAAG TATTTCCCGG TAAGCAGCGG GGACAAACAC TTTCTTGTGC CTCTGTAGAA 
GATCTCACAG CCTTCAGAGT ACCTTTAACT CAACAGTCTT GGTGTATATT AGAGTTCGCG 
TGTCGAGAAG TACACGAAAG TAGTGGCGAC TGGGTTATAT ATTTTACAAA TTATACCGAA 
CTGTGTACCT TCAGAGAAAT TTTGGAAACG TTGTGGGCTG ATGCAAGTTT GGGGGAATTT 
CCAATGGTAA CACTTCCACC AGAAGATAAG CTTCATCGGC GTTGCTCAGA TGCCAGTAAA 
GAGCTTGAGC TTGCATGGCG ATATTTATTA TCTTCTGTAA TTGACTAA