LOC100877705 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877705
Entrez Gene ID	100877705
Full Name	beta-galactosidase-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003707130.2	XP_003707178.1	beta-galactosidase-like

Pathways from BioSystems
Gene Ontology

The following LOC100877705 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877705 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe112555	XM_003707130.2 Latest version!	Megachile rotundata beta-galactosidase-like (LOC100877705), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877705 ( XM_003707130.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003707178.1 Megachile rotundata beta-galactosidase-like (LOC100877705), mRNA.

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CloneID	OMe112555
Clone ID Related Accession (Same CDS sequence)	XM_003707130.2
Accession Version	XM_003707130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	beta-galactosidase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797510.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCCG TTCTGCTAAT TTTGGCAATA ACCGGCGCTA TGGGAGAGGT GGTCAATCGT 
CACGTGGACA ACAGCACAGA CGTAAAATTT GGTTTCGAAG TCGATTATAC GAACAATCAA 
TTTTTACTCG ATGGAAAACC GTTCCGATAC GTTTCTGGTA GTTTTCACTA CTTCCGGACG 
CCGAGGCAAT ATTGGAGAGA TCGTTTAAGG AAAATGAGAG CTGCTGGTTT GAATGCCGTG 
TCGACTTACG TTGAATGGAG TCTTCATCAA CCGGAGGAGG ACCAATGGGA GTGGACAGGT 
GATGCGGATT TAAAGAAATT TATCGAGATT GCTCATGAAG AAGATTTACT GGTTCTTTTG 
AGACCGGGTC CTTATATTTG TGCGGAAAGA GATTTTGGAG GTCTTCCGTA TTGGTTATTG 
CCACGTGTAC CGGTCGACGG ACTACGTACA AACAATGAAC AATACATGCA ATACGTGGAA 
GTGTATCTGA ACGAAGTACT AGAACGCGTT AAACCGTATC TCAGAGGAAA TGGGGGTCCC 
ATAATAATGG TTCAGGTAGA AAACGAGTAC GGAAGCTACG GTTGCAGCAT GGAATACAAG 
ACTCATCTGC GGGATATAAT AAGTGCAAAA GTTGGAACAA AGGCTTTGTT GTACACAACC 
GATGGTACGG AGGATACTAT GCTTCGTTGT GGATCTATAC CAAATGTTTA TACCACCATC 
GACTTCGGAG CTAATAGCAA TGTGAGGAAA AATTTTGAAA TGCTCCGCAC TCATCAGCCA 
CGTGGACCGT TGGTGAATTC CGAATTTTAC CCCGGTTGGC TGTCTCACTG GCAAGAACCA 
TTTCAAAGGG TAAAAACCGG AGTAGTCACC AAAAGCTTAG ACGAAATATT GTCTATGGGT 
GCATCAGTCA ACATATACAT GTTTTATGGC GGTACAAATT TCGCTTACAC CTCTGGTGCT 
AATGGCGATA GAAACATGTA CAATCCACAA TTAACTTCCT ACGATTATGA TGCTCCATTA 
ACGGAAGCAG GTGATCCGAC GACAAAGTAT TTTGAAATTA GAAACGTCAT CTCTAAGTAT 
TTGCCGCTGC CAAATACATC AATTCCATCA GTATCCCCCA AAGGAGATTA CGGTTCCGTT 
CTCCTTTCTC CAGTAGTGAA ATTATTAGAA CCCTTGGGTG AAGGATTGTT TTCAACCGTA 
GCAGTGGAAG CACCAGAGCC GTTGACATTC GAAACAGTTG GACTACCTCA TTATCTAATG 
ATCTACGAAA CCACTTTACC GTCTACTATT TCGGATCCTG CGATCCTGAG TGCTATAGTT 
CGAGACAGAG CGTTGGTCTA CGTTGATAAT TATCTGTTTG GAATCTTAAG TCGTACCAGT 
GAAATACACA GCTTGAACAT AAACCCTTAT GGACGGAAAC TGAAGCTACT GGTTGAAAAT 
CAAGGCCGTT TGAATTTTGG ACATGGGCTT TACGATTATA AGGGAATCTC GAACGTAACT 
TTTGGAGACG CTACGCTTGG ACCCTGGAGA ATGGTTGGAT ATCAATTAGA CGACATGAGT 
GGGGTTAGCG ATCTCGAAAC GATCACGATA GAAAATGGAT TTCTTCGTAA CGGTCCAGTA 
GTTCTTCGTG GAAATTTCTC TGTCTCCGGT GAACCGATGG ACACGTACTT GAACACATTC 
GGTTGGGGCA AAGGGGTGGC TTTCGTGAAC GGGCACAACT TAGGTCGATA CTGGCCGGTG 
TCTGGGCCTC AAGTGACCTT GTACGTTCCT GCACCGTTTT TGAAAACGGG TGAAAATGAA 
TTGATAGTGT TGGAACTTGA ATACGTACCG AACACGAGGA AAATGAAGTT ACAACCTCAA 
CCGATCCTCG ATTATAAACC AATACTCGAG CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003707178.1
CDS	304..2199
Translation	MWSVLLILAITGAMGEVVNRHVDNSTDVKFGFEVDYTNNQFLLDGKPFRYVSGSFHYFRTPRQYWRDRLRKMRAAGLNAVSTYVEWSLHQPEEDQWEWTGDADLKKFIEIAHEEDLLVLLRPGPYICAERDFGGLPYWLLPRVPVDGLRTNNEQYMQYVEVYLNEVLERVKPYLRGNGGPIIMVQVENEYGSYGCSMEYKTHLRDIISAKVGTKALLYTTDGTEDTMLRCGSIPNVYTTIDFGANSNVRKNFEMLRTHQPRGPLVNSEFYPGWLSHWQEPFQRVKTGVVTKSLDEILSMGASVNIYMFYGGTNFAYTSGANGDRNMYNPQLTSYDYDAPLTEAGDPTTKYFEIRNVISKYLPLPNTSIPSVSPKGDYGSVLLSPVVKLLEPLGEGLFSTVAVEAPEPLTFETVGLPHYLMIYETTLPSTISDPAILSAIVRDRALVYVDNYLFGILSRTSEIHSLNINPYGRKLKLLVENQGRLNFGHGLYDYKGISNVTFGDATLGPWRMVGYQLDDMSGVSDLETITIENGFLRNGPVVLRGNFSVSGEPMDTYLNTFGWGKGVAFVNGHNLGRYWPVSGPQVTLYVPAPFLKTGENELIVLELEYVPNTRKMKLQPQPILDYKPILEQ

Target ORF information:

RefSeq Version	XM_003707130.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata beta-galactosidase-like (LOC100877705), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707130.2

ORF Insert Sequence:

ATGTGGTCCG TTCTGCTAAT TTTGGCAATA ACCGGCGCTA TGGGAGAGGT GGTCAATCGT 
CACGTGGACA ACAGCACAGA CGTAAAATTT GGTTTCGAAG TCGATTATAC GAACAATCAA 
TTTTTACTCG ATGGAAAACC GTTCCGATAC GTTTCTGGTA GTTTTCACTA CTTCCGGACG 
CCGAGGCAAT ATTGGAGAGA TCGTTTAAGG AAAATGAGAG CTGCTGGTTT GAATGCCGTG 
TCGACTTACG TTGAATGGAG TCTTCATCAA CCGGAGGAGG ACCAATGGGA GTGGACAGGT 
GATGCGGATT TAAAGAAATT TATCGAGATT GCTCATGAAG AAGATTTACT GGTTCTTTTG 
AGACCGGGTC CTTATATTTG TGCGGAAAGA GATTTTGGAG GTCTTCCGTA TTGGTTATTG 
CCACGTGTAC CGGTCGACGG ACTACGTACA AACAATGAAC AATACATGCA ATACGTGGAA 
GTGTATCTGA ACGAAGTACT AGAACGCGTT AAACCGTATC TCAGAGGAAA TGGGGGTCCC 
ATAATAATGG TTCAGGTAGA AAACGAGTAC GGAAGCTACG GTTGCAGCAT GGAATACAAG 
ACTCATCTGC GGGATATAAT AAGTGCAAAA GTTGGAACAA AGGCTTTGTT GTACACAACC 
GATGGTACGG AGGATACTAT GCTTCGTTGT GGATCTATAC CAAATGTTTA TACCACCATC 
GACTTCGGAG CTAATAGCAA TGTGAGGAAA AATTTTGAAA TGCTCCGCAC TCATCAGCCA 
CGTGGACCGT TGGTGAATTC CGAATTTTAC CCCGGTTGGC TGTCTCACTG GCAAGAACCA 
TTTCAAAGGG TAAAAACCGG AGTAGTCACC AAAAGCTTAG ACGAAATATT GTCTATGGGT 
GCATCAGTCA ACATATACAT GTTTTATGGC GGTACAAATT TCGCTTACAC CTCTGGTGCT 
AATGGCGATA GAAACATGTA CAATCCACAA TTAACTTCCT ACGATTATGA TGCTCCATTA 
ACGGAAGCAG GTGATCCGAC GACAAAGTAT TTTGAAATTA GAAACGTCAT CTCTAAGTAT 
TTGCCGCTGC CAAATACATC AATTCCATCA GTATCCCCCA AAGGAGATTA CGGTTCCGTT 
CTCCTTTCTC CAGTAGTGAA ATTATTAGAA CCCTTGGGTG AAGGATTGTT TTCAACCGTA 
GCAGTGGAAG CACCAGAGCC GTTGACATTC GAAACAGTTG GACTACCTCA TTATCTAATG 
ATCTACGAAA CCACTTTACC GTCTACTATT TCGGATCCTG CGATCCTGAG TGCTATAGTT 
CGAGACAGAG CGTTGGTCTA CGTTGATAAT TATCTGTTTG GAATCTTAAG TCGTACCAGT 
GAAATACACA GCTTGAACAT AAACCCTTAT GGACGGAAAC TGAAGCTACT GGTTGAAAAT 
CAAGGCCGTT TGAATTTTGG ACATGGGCTT TACGATTATA AGGGAATCTC GAACGTAACT 
TTTGGAGACG CTACGCTTGG ACCCTGGAGA ATGGTTGGAT ATCAATTAGA CGACATGAGT 
GGGGTTAGCG ATCTCGAAAC GATCACGATA GAAAATGGAT TTCTTCGTAA CGGTCCAGTA 
GTTCTTCGTG GAAATTTCTC TGTCTCCGGT GAACCGATGG ACACGTACTT GAACACATTC 
GGTTGGGGCA AAGGGGTGGC TTTCGTGAAC GGGCACAACT TAGGTCGATA CTGGCCGGTG 
TCTGGGCCTC AAGTGACCTT GTACGTTCCT GCACCGTTTT TGAAAACGGG TGAAAATGAA 
TTGATAGTGT TGGAACTTGA ATACGTACCG AACACGAGGA AAATGAAGTT ACAACCTCAA 
CCGATCCTCG ATTATAAACC AATACTCGAG CAATGA