LOC100877015 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877015
Entrez Gene ID	100877015
Full Name	ubiquitin carboxyl-terminal hydrolase 47
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012282618.1	XP_012138008.1	ubiquitin carboxyl-terminal hydrolase 47
XM_012282617.1	XP_012138007.1	ubiquitin carboxyl-terminal hydrolase 47

Pathways from BioSystems
Gene Ontology

The following LOC100877015 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877015 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe102274	XM_012282617.1 Latest version!	Megachile rotundata ubiquitin carboxyl-terminal hydrolase 47 (LOC100877015), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OMe102274	XM_012282618.1 Latest version!	Megachile rotundata ubiquitin carboxyl-terminal hydrolase 47 (LOC100877015), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877015 ( XM_012282618.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012138008.1 Megachile rotundata ubiquitin carboxyl-terminal hydrolase 47 (LOC100877015), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe102274
Clone ID Related Accession (Same CDS sequence)	XM_012282618.1 , XM_012282617.1
Accession Version	XM_012282618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ubiquitin carboxyl-terminal hydrolase 47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797147.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGTA TAATTGGTCA ACCATGTCTG ATTGACTTCA ATCTATATCA TGGCGCCAAA 
GATAAGAACC TTTCTAACAT CAAATTTGTA TTAAGTGAAT CTACTCGTGG TAAAGAGCTT 
TACAATTATA TTCAAGAGCT TGGACAAATA CAACCCGACA GTTTTAAATT ATATTTGGTG 
TCAGCAACAG GAACGATTGA TTTGTCTGAA ATGAAGGATA AAACAATGTC AGAAATTGGC 
GTGGATTTTA CCTCTGATCG AGACTCGACT GAATATAAGC ATACACTTTT AGTTCTGGAT 
CCTGTGGCTA GTTTCTGTGC CAATAGTGTG GCACAAAAAC AAGATGAATG TAATCTACCT 
ACATATAATC CTCCACCTCC ATCACCTAAT GATAAGATTG CAGTGCGCAG ATACACTGTT 
AACCCGAATA GTTACAAGGA CCAGGTCTCT GTGGAAAATT ACTCTCCTGA ACCTGAAATA 
CGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAACA 
TGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAATA 
TGTTATGTTG GTTTAGTGAA CCAAGCTATG ACTTGTTATT TAAATAGTCT ACTGCAGGCA 
CTGTATATGA CTCCTGAATT TCGAAATGCC CTGTACAATT GGGAGTATGT AGAAGGGTCA 
GAGAAAGATG AAGCTAATAG TATACCATAT CAGTTACAAA AGTTATTTCT AAACTTGCAG 
ACATCATCAA AATCTGCAGT AGAAACCACA GCATTAACAA AAAGCTTTGG ATGGGATTCT 
ACAGAAGCCT GGCAGCAACA TGATATTCAA GAACTTTGTA GAGTTATGTT TGACGCTTTA 
GAACAAAAGT TTAAGAATAC AGAACAAGCT GATTTAATCA ACAGATTCTA TGAAGGAAAG 
ATGATTGATT ATGTTAAATG TCTTGAATGT GGTACTGAGA AATCTAGAGA AGATACTTTT 
CTTGATATTC CATTACCAGT AAGACCATTT GGTAGTAATG TGGCATATAA TAGCGTGGAA 
GAAGCTTTAA GAGCGTTTGT ACAGTATGAA ACCTTGGAAG AAAATAATCA ATATTTTTGT 
GAAAAATGTA ATAAAAAGTG TGATGCTCAT AAGGGATTAA AATTTACAAA ATTTCCATAT 
CTTCTGACAC TACATCTCAA ACGGTTTGAT TTTGATTTCA AAACTTTTCA CAGAATTAAA 
CTCAATGATA AGGTTACTTT TCCAGATATA CTTAATTTAA ACCCTTTTAT ATCTTCGACA 
CCAAATCAAG AAACTCCAAA TAGTGATGAA AATATTGGTT TAGTAAAATG CGACGATAGT 
TCAACAACAG ATAGTGGTAC ATTAGATGAT GATTATGTGC TTTGTGAAAA TAATCTTTCT 
AATAGTAATC ACTGGACAAA TCACGATCAA GATGACGATG AAGGTATAGA CATGAGTAAT 
GGGCCTTCAA CGTCAAATTG CACAGTACAA AATCATGAAA ATGAAAAAAA TCGTAGTGGG 
AGTATTGGCA AAGGACCTTA TAATTATGAA TTATTTTCAA TTATGATTCA TAGTGGTAGC 
GCTAGTGGTG GTCATTACTA TGCATACATA AAAGATTTCA GAACACAGGA ATGGTTTTGT 
TTTAACGACC AAAGCGTAAC TCAGATAACT CATGATGATA TTCAAAAAAC ATATGGTGGT 
GGTGGTGCAA GCAGATACTG TAGGGTATCA AGTAGTTGTG CCAATGCGTA CATGCTTATG 
TACCGCCAAA TCGATCCTGC TCGTAACGCT TTACCCATGC AGGTCCAAGA TTTTCCGAAA 
CACATACAGG AATTACTGAA AAAAATGAAA GGAGATGAAA CGACGAGATG GAATAACGAT 
AGGAAAATAC AATTGGTTGA TAACTCTCGT TTCATACAAC AAAGAACTCA TAAAGTTTTG 
CCGAAACAAA AATTTTTTTC GCGACACAAA ACTGGCATAT CAGGTTGCAT GGATAAAATA 
AAAGTGCTGT ACAAAGCATC TTTCAATGAA AAAATAATAC TAAACGATCG ACTTCCCATA 
ATGCCTGATG CAACTTTGGC AGAAATAACA AAGCAAGTTT ATAACAAATT CCGTCTAGAA 
AATAAAGTAG ACTTAGATCA GTGCCGTTTA GTTGCTTACG ACGACCATAA TCATATAATC 
CTGACTAGTT TCGAAGGAAG AGAAAATGAA ACATTTGAAA GTATCTGGAA TTCTCGAATG 
AATAGCTTGT TATTAGAATC ACGTGACAAA GATCAAAAGT TCGAATCATA TCCAAAAGGT 
ATCGTTACAA AAGTATTTAT TGTTGATGTA CCCACAAGAG AAGTTATAGA AGGGCCAATA 
ACTGTACAAG CATCGCACAC ACAAACCGTA AGAGAATATA AACAAATGGT GGGAAAGAAG 
GTTAATATGG ATCTCACTGA CATGGAGATT ATTTTGAGAA AACGTGGATC ATCTGCTATA 
CTTGTATCAA ATGATGATCT TGAACTTCAA GCTGCAGAGT TTTGTGATTT GGTAAATGTG 
TTCGTTTGTA GCATGGCAGA TGCTGAATAT AAACCTTTCC TTAAGACAGC GATATATGAC 
ATTGTAAAAG AATTAAAAAA CATCATAATA TTACGTATAA AATTACCCGA TACAAGTAAA 
GAAACGTTGG AAGAACTAAA TATTCCAGCA TTAGAAGAGG AACCTACGGA AAAAGACATA 
TCTGCTACGA CTAGTCCATC CAAAATAAAT GTTAACGATT CGAATCATGA TGCTGGAGTG 
AAATGTAACG AAGTGTCAAA AAGTGGAAAA ACTACCGAAG ATACTCCTGT AAGAAATACG 
AGTCCTCAGT TGGGGGAAGG TGAAGAATGG AATACCCCTG AACAGAGTAA CAGCGAGGAT 
AGTAGTCTCA GTGACAGTGA TAAAACATTG GTTGGAGATG CACCAGAAGA TGATATTTTT 
CAATCACCGT ATGGTAAAAT TATCATATCA GAAAACCGTG CCATTAAAAT GTTGAATCAA 
GCCGGACTCG AGAATCCGGA CAATATCATT GACGATTCTG AATATTATTT CAAAGCCATC 
CCTTACGTCA GTAGTTACGG AAAATTTATA CACGTACTGG TTGACAAAAG GATGATATTC 
AGCACCTTAA AGAAACATTT GGAATCGTAT GTCGGTGTAC CTTCAGAATA TTTCAAAATA 
GCTCGTTATT TCGATGAATC TAGCGATTCT GAATGTAATT TATTTTGTCG ACTTGTTTCT 
TTTTATGAGG ATAGCGAGGA ACTTTTTATA AGGCTTGGAC GAGCTCTACG TAACGGTGAA 
TTTCCAGTAA CATTATATCA GTTTCGTATT AATTCACTCG AGCCTTACAA ATTCTTGTGT 
GAATCGGTTC TTTCGGAGGG TATGCTGGTT AGTGAAGCAA AAAAAGTACT TTTGGCAGAA 
ATTAAAAGAA AGTGCGACAT AGATATTCCA TATGAAAGGT GTCGATTGAG GAAAAAGTGC 
TTTAGTAGAG CCTCAAAAGT ATTTTTAGAC GATCAGAAAT TCAAATTTCC ATTTTTCTCA 
CAATTCGAAT TGATCATACA AGAGTTACCT GGTAAGGATC CAGTTACCAA CAGCAACCAA 
GTTGTTGTAT TCCTTCAGAG ATGGTCAATT GAAGAAATGC AACTTAAACC GCTCGAAGAA 
ATTGTTTTGG ATGGAAAAAC TGCAAAGGAA TTGTACAAAA AAGTGTCTTC GATTTCGGAA 
ATCCCTGAAA ATCACTTAGA CATAGCAAAA GGAGTAAGGG GTTCTGTCCC ATATACGTTT 
ATCTCTCAAA TTCAGGATGA AGTAGACTGG GAGGAGACTC ATGCTGATAT TGAAGTTAGT 
ATTCACGATG GCGCTCTATT CATATATAGG GATAACAGGG AAATGGCGAA AAATGTACCG 
CGGGGGGAGG TCACTGATCT ACCATTTCAT CAAGATGTAT CTTTTTTATT ATCACCTTCC 
TCTACGCCAC GTTATAGTGG GCGAAAAGAG AAAGCACTTA GAATAAATTT GGATGCCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012138008.1
CDS	533..4615
Translation	MVCIIGQPCLIDFNLYHGAKDKNLSNIKFVLSESTRGKELYNYIQELGQIQPDSFKLYLVSATGTIDLSEMKDKTMSEIGVDFTSDRDSTEYKHTLLVLDPVASFCANSVAQKQDECNLPTYNPPPPSPNDKIAVRRYTVNPNSYKDQVSVENYSPEPEIRYFGVKKKVSEENHSPEPETCYFGVKKKVSEENHSPEPEICYVGLVNQAMTCYLNSLLQALYMTPEFRNALYNWEYVEGSEKDEANSIPYQLQKLFLNLQTSSKSAVETTALTKSFGWDSTEAWQQHDIQELCRVMFDALEQKFKNTEQADLINRFYEGKMIDYVKCLECGTEKSREDTFLDIPLPVRPFGSNVAYNSVEEALRAFVQYETLEENNQYFCEKCNKKCDAHKGLKFTKFPYLLTLHLKRFDFDFKTFHRIKLNDKVTFPDILNLNPFISSTPNQETPNSDENIGLVKCDDSSTTDSGTLDDDYVLCENNLSNSNHWTNHDQDDDEGIDMSNGPSTSNCTVQNHENEKNRSGSIGKGPYNYELFSIMIHSGSASGGHYYAYIKDFRTQEWFCFNDQSVTQITHDDIQKTYGGGGASRYCRVSSSCANAYMLMYRQIDPARNALPMQVQDFPKHIQELLKKMKGDETTRWNNDRKIQLVDNSRFIQQRTHKVLPKQKFFSRHKTGISGCMDKIKVLYKASFNEKIILNDRLPIMPDATLAEITKQVYNKFRLENKVDLDQCRLVAYDDHNHIILTSFEGRENETFESIWNSRMNSLLLESRDKDQKFESYPKGIVTKVFIVDVPTREVIEGPITVQASHTQTVREYKQMVGKKVNMDLTDMEIILRKRGSSAILVSNDDLELQAAEFCDLVNVFVCSMADAEYKPFLKTAIYDIVKELKNIIILRIKLPDTSKETLEELNIPALEEEPTEKDISATTSPSKINVNDSNHDAGVKCNEVSKSGKTTEDTPVRNTSPQLGEGEEWNTPEQSNSEDSSLSDSDKTLVGDAPEDDIFQSPYGKIIISENRAIKMLNQAGLENPDNIIDDSEYYFKAIPYVSSYGKFIHVLVDKRMIFSTLKKHLESYVGVPSEYFKIARYFDESSDSECNLFCRLVSFYEDSEELFIRLGRALRNGEFPVTLYQFRINSLEPYKFLCESVLSEGMLVSEAKKVLLAEIKRKCDIDIPYERCRLRKKCFSRASKVFLDDQKFKFPFFSQFELIIQELPGKDPVTNSNQVVVFLQRWSIEEMQLKPLEEIVLDGKTAKELYKKVSSISEIPENHLDIAKGVRGSVPYTFISQIQDEVDWEETHADIEVSIHDGALFIYRDNREMAKNVPRGEVTDLPFHQDVSFLLSPSSTPRYSGRKEKALRINLDAE

Target ORF information:

RefSeq Version	XM_012282618.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ubiquitin carboxyl-terminal hydrolase 47 (LOC100877015), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012282618.1

ORF Insert Sequence:

ATGGTTTGTA TAATTGGTCA ACCATGTCTG ATTGACTTCA ATCTATATCA TGGCGCCAAA 
GATAAGAACC TTTCTAACAT CAAATTTGTA TTAAGTGAAT CTACTCGTGG TAAAGAGCTT 
TACAATTATA TTCAAGAGCT TGGACAAATA CAACCCGACA GTTTTAAATT ATATTTGGTG 
TCAGCAACAG GAACGATTGA TTTGTCTGAA ATGAAGGATA AAACAATGTC AGAAATTGGC 
GTGGATTTTA CCTCTGATCG AGACTCGACT GAATATAAGC ATACACTTTT AGTTCTGGAT 
CCTGTGGCTA GTTTCTGTGC CAATAGTGTG GCACAAAAAC AAGATGAATG TAATCTACCT 
ACATATAATC CTCCACCTCC ATCACCTAAT GATAAGATTG CAGTGCGCAG ATACACTGTT 
AACCCGAATA GTTACAAGGA CCAGGTCTCT GTGGAAAATT ACTCTCCTGA ACCTGAAATA 
CGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAACA 
TGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAATA 
TGTTATGTTG GTTTAGTGAA CCAAGCTATG ACTTGTTATT TAAATAGTCT ACTGCAGGCA 
CTGTATATGA CTCCTGAATT TCGAAATGCC CTGTACAATT GGGAGTATGT AGAAGGGTCA 
GAGAAAGATG AAGCTAATAG TATACCATAT CAGTTACAAA AGTTATTTCT AAACTTGCAG 
ACATCATCAA AATCTGCAGT AGAAACCACA GCATTAACAA AAAGCTTTGG ATGGGATTCT 
ACAGAAGCCT GGCAGCAACA TGATATTCAA GAACTTTGTA GAGTTATGTT TGACGCTTTA 
GAACAAAAGT TTAAGAATAC AGAACAAGCT GATTTAATCA ACAGATTCTA TGAAGGAAAG 
ATGATTGATT ATGTTAAATG TCTTGAATGT GGTACTGAGA AATCTAGAGA AGATACTTTT 
CTTGATATTC CATTACCAGT AAGACCATTT GGTAGTAATG TGGCATATAA TAGCGTGGAA 
GAAGCTTTAA GAGCGTTTGT ACAGTATGAA ACCTTGGAAG AAAATAATCA ATATTTTTGT 
GAAAAATGTA ATAAAAAGTG TGATGCTCAT AAGGGATTAA AATTTACAAA ATTTCCATAT 
CTTCTGACAC TACATCTCAA ACGGTTTGAT TTTGATTTCA AAACTTTTCA CAGAATTAAA 
CTCAATGATA AGGTTACTTT TCCAGATATA CTTAATTTAA ACCCTTTTAT ATCTTCGACA 
CCAAATCAAG AAACTCCAAA TAGTGATGAA AATATTGGTT TAGTAAAATG CGACGATAGT 
TCAACAACAG ATAGTGGTAC ATTAGATGAT GATTATGTGC TTTGTGAAAA TAATCTTTCT 
AATAGTAATC ACTGGACAAA TCACGATCAA GATGACGATG AAGGTATAGA CATGAGTAAT 
GGGCCTTCAA CGTCAAATTG CACAGTACAA AATCATGAAA ATGAAAAAAA TCGTAGTGGG 
AGTATTGGCA AAGGACCTTA TAATTATGAA TTATTTTCAA TTATGATTCA TAGTGGTAGC 
GCTAGTGGTG GTCATTACTA TGCATACATA AAAGATTTCA GAACACAGGA ATGGTTTTGT 
TTTAACGACC AAAGCGTAAC TCAGATAACT CATGATGATA TTCAAAAAAC ATATGGTGGT 
GGTGGTGCAA GCAGATACTG TAGGGTATCA AGTAGTTGTG CCAATGCGTA CATGCTTATG 
TACCGCCAAA TCGATCCTGC TCGTAACGCT TTACCCATGC AGGTCCAAGA TTTTCCGAAA 
CACATACAGG AATTACTGAA AAAAATGAAA GGAGATGAAA CGACGAGATG GAATAACGAT 
AGGAAAATAC AATTGGTTGA TAACTCTCGT TTCATACAAC AAAGAACTCA TAAAGTTTTG 
CCGAAACAAA AATTTTTTTC GCGACACAAA ACTGGCATAT CAGGTTGCAT GGATAAAATA 
AAAGTGCTGT ACAAAGCATC TTTCAATGAA AAAATAATAC TAAACGATCG ACTTCCCATA 
ATGCCTGATG CAACTTTGGC AGAAATAACA AAGCAAGTTT ATAACAAATT CCGTCTAGAA 
AATAAAGTAG ACTTAGATCA GTGCCGTTTA GTTGCTTACG ACGACCATAA TCATATAATC 
CTGACTAGTT TCGAAGGAAG AGAAAATGAA ACATTTGAAA GTATCTGGAA TTCTCGAATG 
AATAGCTTGT TATTAGAATC ACGTGACAAA GATCAAAAGT TCGAATCATA TCCAAAAGGT 
ATCGTTACAA AAGTATTTAT TGTTGATGTA CCCACAAGAG AAGTTATAGA AGGGCCAATA 
ACTGTACAAG CATCGCACAC ACAAACCGTA AGAGAATATA AACAAATGGT GGGAAAGAAG 
GTTAATATGG ATCTCACTGA CATGGAGATT ATTTTGAGAA AACGTGGATC ATCTGCTATA 
CTTGTATCAA ATGATGATCT TGAACTTCAA GCTGCAGAGT TTTGTGATTT GGTAAATGTG 
TTCGTTTGTA GCATGGCAGA TGCTGAATAT AAACCTTTCC TTAAGACAGC GATATATGAC 
ATTGTAAAAG AATTAAAAAA CATCATAATA TTACGTATAA AATTACCCGA TACAAGTAAA 
GAAACGTTGG AAGAACTAAA TATTCCAGCA TTAGAAGAGG AACCTACGGA AAAAGACATA 
TCTGCTACGA CTAGTCCATC CAAAATAAAT GTTAACGATT CGAATCATGA TGCTGGAGTG 
AAATGTAACG AAGTGTCAAA AAGTGGAAAA ACTACCGAAG ATACTCCTGT AAGAAATACG 
AGTCCTCAGT TGGGGGAAGG TGAAGAATGG AATACCCCTG AACAGAGTAA CAGCGAGGAT 
AGTAGTCTCA GTGACAGTGA TAAAACATTG GTTGGAGATG CACCAGAAGA TGATATTTTT 
CAATCACCGT ATGGTAAAAT TATCATATCA GAAAACCGTG CCATTAAAAT GTTGAATCAA 
GCCGGACTCG AGAATCCGGA CAATATCATT GACGATTCTG AATATTATTT CAAAGCCATC 
CCTTACGTCA GTAGTTACGG AAAATTTATA CACGTACTGG TTGACAAAAG GATGATATTC 
AGCACCTTAA AGAAACATTT GGAATCGTAT GTCGGTGTAC CTTCAGAATA TTTCAAAATA 
GCTCGTTATT TCGATGAATC TAGCGATTCT GAATGTAATT TATTTTGTCG ACTTGTTTCT 
TTTTATGAGG ATAGCGAGGA ACTTTTTATA AGGCTTGGAC GAGCTCTACG TAACGGTGAA 
TTTCCAGTAA CATTATATCA GTTTCGTATT AATTCACTCG AGCCTTACAA ATTCTTGTGT 
GAATCGGTTC TTTCGGAGGG TATGCTGGTT AGTGAAGCAA AAAAAGTACT TTTGGCAGAA 
ATTAAAAGAA AGTGCGACAT AGATATTCCA TATGAAAGGT GTCGATTGAG GAAAAAGTGC 
TTTAGTAGAG CCTCAAAAGT ATTTTTAGAC GATCAGAAAT TCAAATTTCC ATTTTTCTCA 
CAATTCGAAT TGATCATACA AGAGTTACCT GGTAAGGATC CAGTTACCAA CAGCAACCAA 
GTTGTTGTAT TCCTTCAGAG ATGGTCAATT GAAGAAATGC AACTTAAACC GCTCGAAGAA 
ATTGTTTTGG ATGGAAAAAC TGCAAAGGAA TTGTACAAAA AAGTGTCTTC GATTTCGGAA 
ATCCCTGAAA ATCACTTAGA CATAGCAAAA GGAGTAAGGG GTTCTGTCCC ATATACGTTT 
ATCTCTCAAA TTCAGGATGA AGTAGACTGG GAGGAGACTC ATGCTGATAT TGAAGTTAGT 
ATTCACGATG GCGCTCTATT CATATATAGG GATAACAGGG AAATGGCGAA AAATGTACCG 
CGGGGGGAGG TCACTGATCT ACCATTTCAT CAAGATGTAT CTTTTTTATT ATCACCTTCC 
TCTACGCCAC GTTATAGTGG GCGAAAAGAG AAAGCACTTA GAATAAATTT GGATGCCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877015 ( XM_012282617.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012138007.1 Megachile rotundata ubiquitin carboxyl-terminal hydrolase 47 (LOC100877015), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe102274
Clone ID Related Accession (Same CDS sequence)	XM_012282618.1 , XM_012282617.1
Accession Version	XM_012282617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ubiquitin carboxyl-terminal hydrolase 47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797147.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGTA TAATTGGTCA ACCATGTCTG ATTGACTTCA ATCTATATCA TGGCGCCAAA 
GATAAGAACC TTTCTAACAT CAAATTTGTA TTAAGTGAAT CTACTCGTGG TAAAGAGCTT 
TACAATTATA TTCAAGAGCT TGGACAAATA CAACCCGACA GTTTTAAATT ATATTTGGTG 
TCAGCAACAG GAACGATTGA TTTGTCTGAA ATGAAGGATA AAACAATGTC AGAAATTGGC 
GTGGATTTTA CCTCTGATCG AGACTCGACT GAATATAAGC ATACACTTTT AGTTCTGGAT 
CCTGTGGCTA GTTTCTGTGC CAATAGTGTG GCACAAAAAC AAGATGAATG TAATCTACCT 
ACATATAATC CTCCACCTCC ATCACCTAAT GATAAGATTG CAGTGCGCAG ATACACTGTT 
AACCCGAATA GTTACAAGGA CCAGGTCTCT GTGGAAAATT ACTCTCCTGA ACCTGAAATA 
CGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAACA 
TGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAATA 
TGTTATGTTG GTTTAGTGAA CCAAGCTATG ACTTGTTATT TAAATAGTCT ACTGCAGGCA 
CTGTATATGA CTCCTGAATT TCGAAATGCC CTGTACAATT GGGAGTATGT AGAAGGGTCA 
GAGAAAGATG AAGCTAATAG TATACCATAT CAGTTACAAA AGTTATTTCT AAACTTGCAG 
ACATCATCAA AATCTGCAGT AGAAACCACA GCATTAACAA AAAGCTTTGG ATGGGATTCT 
ACAGAAGCCT GGCAGCAACA TGATATTCAA GAACTTTGTA GAGTTATGTT TGACGCTTTA 
GAACAAAAGT TTAAGAATAC AGAACAAGCT GATTTAATCA ACAGATTCTA TGAAGGAAAG 
ATGATTGATT ATGTTAAATG TCTTGAATGT GGTACTGAGA AATCTAGAGA AGATACTTTT 
CTTGATATTC CATTACCAGT AAGACCATTT GGTAGTAATG TGGCATATAA TAGCGTGGAA 
GAAGCTTTAA GAGCGTTTGT ACAGTATGAA ACCTTGGAAG AAAATAATCA ATATTTTTGT 
GAAAAATGTA ATAAAAAGTG TGATGCTCAT AAGGGATTAA AATTTACAAA ATTTCCATAT 
CTTCTGACAC TACATCTCAA ACGGTTTGAT TTTGATTTCA AAACTTTTCA CAGAATTAAA 
CTCAATGATA AGGTTACTTT TCCAGATATA CTTAATTTAA ACCCTTTTAT ATCTTCGACA 
CCAAATCAAG AAACTCCAAA TAGTGATGAA AATATTGGTT TAGTAAAATG CGACGATAGT 
TCAACAACAG ATAGTGGTAC ATTAGATGAT GATTATGTGC TTTGTGAAAA TAATCTTTCT 
AATAGTAATC ACTGGACAAA TCACGATCAA GATGACGATG AAGGTATAGA CATGAGTAAT 
GGGCCTTCAA CGTCAAATTG CACAGTACAA AATCATGAAA ATGAAAAAAA TCGTAGTGGG 
AGTATTGGCA AAGGACCTTA TAATTATGAA TTATTTTCAA TTATGATTCA TAGTGGTAGC 
GCTAGTGGTG GTCATTACTA TGCATACATA AAAGATTTCA GAACACAGGA ATGGTTTTGT 
TTTAACGACC AAAGCGTAAC TCAGATAACT CATGATGATA TTCAAAAAAC ATATGGTGGT 
GGTGGTGCAA GCAGATACTG TAGGGTATCA AGTAGTTGTG CCAATGCGTA CATGCTTATG 
TACCGCCAAA TCGATCCTGC TCGTAACGCT TTACCCATGC AGGTCCAAGA TTTTCCGAAA 
CACATACAGG AATTACTGAA AAAAATGAAA GGAGATGAAA CGACGAGATG GAATAACGAT 
AGGAAAATAC AATTGGTTGA TAACTCTCGT TTCATACAAC AAAGAACTCA TAAAGTTTTG 
CCGAAACAAA AATTTTTTTC GCGACACAAA ACTGGCATAT CAGGTTGCAT GGATAAAATA 
AAAGTGCTGT ACAAAGCATC TTTCAATGAA AAAATAATAC TAAACGATCG ACTTCCCATA 
ATGCCTGATG CAACTTTGGC AGAAATAACA AAGCAAGTTT ATAACAAATT CCGTCTAGAA 
AATAAAGTAG ACTTAGATCA GTGCCGTTTA GTTGCTTACG ACGACCATAA TCATATAATC 
CTGACTAGTT TCGAAGGAAG AGAAAATGAA ACATTTGAAA GTATCTGGAA TTCTCGAATG 
AATAGCTTGT TATTAGAATC ACGTGACAAA GATCAAAAGT TCGAATCATA TCCAAAAGGT 
ATCGTTACAA AAGTATTTAT TGTTGATGTA CCCACAAGAG AAGTTATAGA AGGGCCAATA 
ACTGTACAAG CATCGCACAC ACAAACCGTA AGAGAATATA AACAAATGGT GGGAAAGAAG 
GTTAATATGG ATCTCACTGA CATGGAGATT ATTTTGAGAA AACGTGGATC ATCTGCTATA 
CTTGTATCAA ATGATGATCT TGAACTTCAA GCTGCAGAGT TTTGTGATTT GGTAAATGTG 
TTCGTTTGTA GCATGGCAGA TGCTGAATAT AAACCTTTCC TTAAGACAGC GATATATGAC 
ATTGTAAAAG AATTAAAAAA CATCATAATA TTACGTATAA AATTACCCGA TACAAGTAAA 
GAAACGTTGG AAGAACTAAA TATTCCAGCA TTAGAAGAGG AACCTACGGA AAAAGACATA 
TCTGCTACGA CTAGTCCATC CAAAATAAAT GTTAACGATT CGAATCATGA TGCTGGAGTG 
AAATGTAACG AAGTGTCAAA AAGTGGAAAA ACTACCGAAG ATACTCCTGT AAGAAATACG 
AGTCCTCAGT TGGGGGAAGG TGAAGAATGG AATACCCCTG AACAGAGTAA CAGCGAGGAT 
AGTAGTCTCA GTGACAGTGA TAAAACATTG GTTGGAGATG CACCAGAAGA TGATATTTTT 
CAATCACCGT ATGGTAAAAT TATCATATCA GAAAACCGTG CCATTAAAAT GTTGAATCAA 
GCCGGACTCG AGAATCCGGA CAATATCATT GACGATTCTG AATATTATTT CAAAGCCATC 
CCTTACGTCA GTAGTTACGG AAAATTTATA CACGTACTGG TTGACAAAAG GATGATATTC 
AGCACCTTAA AGAAACATTT GGAATCGTAT GTCGGTGTAC CTTCAGAATA TTTCAAAATA 
GCTCGTTATT TCGATGAATC TAGCGATTCT GAATGTAATT TATTTTGTCG ACTTGTTTCT 
TTTTATGAGG ATAGCGAGGA ACTTTTTATA AGGCTTGGAC GAGCTCTACG TAACGGTGAA 
TTTCCAGTAA CATTATATCA GTTTCGTATT AATTCACTCG AGCCTTACAA ATTCTTGTGT 
GAATCGGTTC TTTCGGAGGG TATGCTGGTT AGTGAAGCAA AAAAAGTACT TTTGGCAGAA 
ATTAAAAGAA AGTGCGACAT AGATATTCCA TATGAAAGGT GTCGATTGAG GAAAAAGTGC 
TTTAGTAGAG CCTCAAAAGT ATTTTTAGAC GATCAGAAAT TCAAATTTCC ATTTTTCTCA 
CAATTCGAAT TGATCATACA AGAGTTACCT GGTAAGGATC CAGTTACCAA CAGCAACCAA 
GTTGTTGTAT TCCTTCAGAG ATGGTCAATT GAAGAAATGC AACTTAAACC GCTCGAAGAA 
ATTGTTTTGG ATGGAAAAAC TGCAAAGGAA TTGTACAAAA AAGTGTCTTC GATTTCGGAA 
ATCCCTGAAA ATCACTTAGA CATAGCAAAA GGAGTAAGGG GTTCTGTCCC ATATACGTTT 
ATCTCTCAAA TTCAGGATGA AGTAGACTGG GAGGAGACTC ATGCTGATAT TGAAGTTAGT 
ATTCACGATG GCGCTCTATT CATATATAGG GATAACAGGG AAATGGCGAA AAATGTACCG 
CGGGGGGAGG TCACTGATCT ACCATTTCAT CAAGATGTAT CTTTTTTATT ATCACCTTCC 
TCTACGCCAC GTTATAGTGG GCGAAAAGAG AAAGCACTTA GAATAAATTT GGATGCCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012138007.1
CDS	187..4269
Translation	MVCIIGQPCLIDFNLYHGAKDKNLSNIKFVLSESTRGKELYNYIQELGQIQPDSFKLYLVSATGTIDLSEMKDKTMSEIGVDFTSDRDSTEYKHTLLVLDPVASFCANSVAQKQDECNLPTYNPPPPSPNDKIAVRRYTVNPNSYKDQVSVENYSPEPEIRYFGVKKKVSEENHSPEPETCYFGVKKKVSEENHSPEPEICYVGLVNQAMTCYLNSLLQALYMTPEFRNALYNWEYVEGSEKDEANSIPYQLQKLFLNLQTSSKSAVETTALTKSFGWDSTEAWQQHDIQELCRVMFDALEQKFKNTEQADLINRFYEGKMIDYVKCLECGTEKSREDTFLDIPLPVRPFGSNVAYNSVEEALRAFVQYETLEENNQYFCEKCNKKCDAHKGLKFTKFPYLLTLHLKRFDFDFKTFHRIKLNDKVTFPDILNLNPFISSTPNQETPNSDENIGLVKCDDSSTTDSGTLDDDYVLCENNLSNSNHWTNHDQDDDEGIDMSNGPSTSNCTVQNHENEKNRSGSIGKGPYNYELFSIMIHSGSASGGHYYAYIKDFRTQEWFCFNDQSVTQITHDDIQKTYGGGGASRYCRVSSSCANAYMLMYRQIDPARNALPMQVQDFPKHIQELLKKMKGDETTRWNNDRKIQLVDNSRFIQQRTHKVLPKQKFFSRHKTGISGCMDKIKVLYKASFNEKIILNDRLPIMPDATLAEITKQVYNKFRLENKVDLDQCRLVAYDDHNHIILTSFEGRENETFESIWNSRMNSLLLESRDKDQKFESYPKGIVTKVFIVDVPTREVIEGPITVQASHTQTVREYKQMVGKKVNMDLTDMEIILRKRGSSAILVSNDDLELQAAEFCDLVNVFVCSMADAEYKPFLKTAIYDIVKELKNIIILRIKLPDTSKETLEELNIPALEEEPTEKDISATTSPSKINVNDSNHDAGVKCNEVSKSGKTTEDTPVRNTSPQLGEGEEWNTPEQSNSEDSSLSDSDKTLVGDAPEDDIFQSPYGKIIISENRAIKMLNQAGLENPDNIIDDSEYYFKAIPYVSSYGKFIHVLVDKRMIFSTLKKHLESYVGVPSEYFKIARYFDESSDSECNLFCRLVSFYEDSEELFIRLGRALRNGEFPVTLYQFRINSLEPYKFLCESVLSEGMLVSEAKKVLLAEIKRKCDIDIPYERCRLRKKCFSRASKVFLDDQKFKFPFFSQFELIIQELPGKDPVTNSNQVVVFLQRWSIEEMQLKPLEEIVLDGKTAKELYKKVSSISEIPENHLDIAKGVRGSVPYTFISQIQDEVDWEETHADIEVSIHDGALFIYRDNREMAKNVPRGEVTDLPFHQDVSFLLSPSSTPRYSGRKEKALRINLDAE

Target ORF information:

RefSeq Version	XM_012282617.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ubiquitin carboxyl-terminal hydrolase 47 (LOC100877015), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012282617.1

ORF Insert Sequence:

ATGGTTTGTA TAATTGGTCA ACCATGTCTG ATTGACTTCA ATCTATATCA TGGCGCCAAA 
GATAAGAACC TTTCTAACAT CAAATTTGTA TTAAGTGAAT CTACTCGTGG TAAAGAGCTT 
TACAATTATA TTCAAGAGCT TGGACAAATA CAACCCGACA GTTTTAAATT ATATTTGGTG 
TCAGCAACAG GAACGATTGA TTTGTCTGAA ATGAAGGATA AAACAATGTC AGAAATTGGC 
GTGGATTTTA CCTCTGATCG AGACTCGACT GAATATAAGC ATACACTTTT AGTTCTGGAT 
CCTGTGGCTA GTTTCTGTGC CAATAGTGTG GCACAAAAAC AAGATGAATG TAATCTACCT 
ACATATAATC CTCCACCTCC ATCACCTAAT GATAAGATTG CAGTGCGCAG ATACACTGTT 
AACCCGAATA GTTACAAGGA CCAGGTCTCT GTGGAAAATT ACTCTCCTGA ACCTGAAATA 
CGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAACA 
TGTTATTTTG GTGTCAAGAA AAAGGTCTCT GAGGAAAATC ACTCTCCTGA ACCTGAAATA 
TGTTATGTTG GTTTAGTGAA CCAAGCTATG ACTTGTTATT TAAATAGTCT ACTGCAGGCA 
CTGTATATGA CTCCTGAATT TCGAAATGCC CTGTACAATT GGGAGTATGT AGAAGGGTCA 
GAGAAAGATG AAGCTAATAG TATACCATAT CAGTTACAAA AGTTATTTCT AAACTTGCAG 
ACATCATCAA AATCTGCAGT AGAAACCACA GCATTAACAA AAAGCTTTGG ATGGGATTCT 
ACAGAAGCCT GGCAGCAACA TGATATTCAA GAACTTTGTA GAGTTATGTT TGACGCTTTA 
GAACAAAAGT TTAAGAATAC AGAACAAGCT GATTTAATCA ACAGATTCTA TGAAGGAAAG 
ATGATTGATT ATGTTAAATG TCTTGAATGT GGTACTGAGA AATCTAGAGA AGATACTTTT 
CTTGATATTC CATTACCAGT AAGACCATTT GGTAGTAATG TGGCATATAA TAGCGTGGAA 
GAAGCTTTAA GAGCGTTTGT ACAGTATGAA ACCTTGGAAG AAAATAATCA ATATTTTTGT 
GAAAAATGTA ATAAAAAGTG TGATGCTCAT AAGGGATTAA AATTTACAAA ATTTCCATAT 
CTTCTGACAC TACATCTCAA ACGGTTTGAT TTTGATTTCA AAACTTTTCA CAGAATTAAA 
CTCAATGATA AGGTTACTTT TCCAGATATA CTTAATTTAA ACCCTTTTAT ATCTTCGACA 
CCAAATCAAG AAACTCCAAA TAGTGATGAA AATATTGGTT TAGTAAAATG CGACGATAGT 
TCAACAACAG ATAGTGGTAC ATTAGATGAT GATTATGTGC TTTGTGAAAA TAATCTTTCT 
AATAGTAATC ACTGGACAAA TCACGATCAA GATGACGATG AAGGTATAGA CATGAGTAAT 
GGGCCTTCAA CGTCAAATTG CACAGTACAA AATCATGAAA ATGAAAAAAA TCGTAGTGGG 
AGTATTGGCA AAGGACCTTA TAATTATGAA TTATTTTCAA TTATGATTCA TAGTGGTAGC 
GCTAGTGGTG GTCATTACTA TGCATACATA AAAGATTTCA GAACACAGGA ATGGTTTTGT 
TTTAACGACC AAAGCGTAAC TCAGATAACT CATGATGATA TTCAAAAAAC ATATGGTGGT 
GGTGGTGCAA GCAGATACTG TAGGGTATCA AGTAGTTGTG CCAATGCGTA CATGCTTATG 
TACCGCCAAA TCGATCCTGC TCGTAACGCT TTACCCATGC AGGTCCAAGA TTTTCCGAAA 
CACATACAGG AATTACTGAA AAAAATGAAA GGAGATGAAA CGACGAGATG GAATAACGAT 
AGGAAAATAC AATTGGTTGA TAACTCTCGT TTCATACAAC AAAGAACTCA TAAAGTTTTG 
CCGAAACAAA AATTTTTTTC GCGACACAAA ACTGGCATAT CAGGTTGCAT GGATAAAATA 
AAAGTGCTGT ACAAAGCATC TTTCAATGAA AAAATAATAC TAAACGATCG ACTTCCCATA 
ATGCCTGATG CAACTTTGGC AGAAATAACA AAGCAAGTTT ATAACAAATT CCGTCTAGAA 
AATAAAGTAG ACTTAGATCA GTGCCGTTTA GTTGCTTACG ACGACCATAA TCATATAATC 
CTGACTAGTT TCGAAGGAAG AGAAAATGAA ACATTTGAAA GTATCTGGAA TTCTCGAATG 
AATAGCTTGT TATTAGAATC ACGTGACAAA GATCAAAAGT TCGAATCATA TCCAAAAGGT 
ATCGTTACAA AAGTATTTAT TGTTGATGTA CCCACAAGAG AAGTTATAGA AGGGCCAATA 
ACTGTACAAG CATCGCACAC ACAAACCGTA AGAGAATATA AACAAATGGT GGGAAAGAAG 
GTTAATATGG ATCTCACTGA CATGGAGATT ATTTTGAGAA AACGTGGATC ATCTGCTATA 
CTTGTATCAA ATGATGATCT TGAACTTCAA GCTGCAGAGT TTTGTGATTT GGTAAATGTG 
TTCGTTTGTA GCATGGCAGA TGCTGAATAT AAACCTTTCC TTAAGACAGC GATATATGAC 
ATTGTAAAAG AATTAAAAAA CATCATAATA TTACGTATAA AATTACCCGA TACAAGTAAA 
GAAACGTTGG AAGAACTAAA TATTCCAGCA TTAGAAGAGG AACCTACGGA AAAAGACATA 
TCTGCTACGA CTAGTCCATC CAAAATAAAT GTTAACGATT CGAATCATGA TGCTGGAGTG 
AAATGTAACG AAGTGTCAAA AAGTGGAAAA ACTACCGAAG ATACTCCTGT AAGAAATACG 
AGTCCTCAGT TGGGGGAAGG TGAAGAATGG AATACCCCTG AACAGAGTAA CAGCGAGGAT 
AGTAGTCTCA GTGACAGTGA TAAAACATTG GTTGGAGATG CACCAGAAGA TGATATTTTT 
CAATCACCGT ATGGTAAAAT TATCATATCA GAAAACCGTG CCATTAAAAT GTTGAATCAA 
GCCGGACTCG AGAATCCGGA CAATATCATT GACGATTCTG AATATTATTT CAAAGCCATC 
CCTTACGTCA GTAGTTACGG AAAATTTATA CACGTACTGG TTGACAAAAG GATGATATTC 
AGCACCTTAA AGAAACATTT GGAATCGTAT GTCGGTGTAC CTTCAGAATA TTTCAAAATA 
GCTCGTTATT TCGATGAATC TAGCGATTCT GAATGTAATT TATTTTGTCG ACTTGTTTCT 
TTTTATGAGG ATAGCGAGGA ACTTTTTATA AGGCTTGGAC GAGCTCTACG TAACGGTGAA 
TTTCCAGTAA CATTATATCA GTTTCGTATT AATTCACTCG AGCCTTACAA ATTCTTGTGT 
GAATCGGTTC TTTCGGAGGG TATGCTGGTT AGTGAAGCAA AAAAAGTACT TTTGGCAGAA 
ATTAAAAGAA AGTGCGACAT AGATATTCCA TATGAAAGGT GTCGATTGAG GAAAAAGTGC 
TTTAGTAGAG CCTCAAAAGT ATTTTTAGAC GATCAGAAAT TCAAATTTCC ATTTTTCTCA 
CAATTCGAAT TGATCATACA AGAGTTACCT GGTAAGGATC CAGTTACCAA CAGCAACCAA 
GTTGTTGTAT TCCTTCAGAG ATGGTCAATT GAAGAAATGC AACTTAAACC GCTCGAAGAA 
ATTGTTTTGG ATGGAAAAAC TGCAAAGGAA TTGTACAAAA AAGTGTCTTC GATTTCGGAA 
ATCCCTGAAA ATCACTTAGA CATAGCAAAA GGAGTAAGGG GTTCTGTCCC ATATACGTTT 
ATCTCTCAAA TTCAGGATGA AGTAGACTGG GAGGAGACTC ATGCTGATAT TGAAGTTAGT 
ATTCACGATG GCGCTCTATT CATATATAGG GATAACAGGG AAATGGCGAA AAATGTACCG 
CGGGGGGAGG TCACTGATCT ACCATTTCAT CAAGATGTAT CTTTTTTATT ATCACCTTCC 
TCTACGCCAC GTTATAGTGG GCGAAAAGAG AAAGCACTTA GAATAAATTT GGATGCCGAA 
TGA