LOC100875727 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100875727
Entrez Gene ID	100875727
Full Name	cullin-4A
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003707602.2	XP_003707650.1	cullin-4A isoform X1
XM_012295281.1	XP_012150671.1	cullin-4B isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100875727 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100875727 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe113403	XM_003707602.2 Latest version!	Megachile rotundata cullin-4A (LOC100875727), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe113404	XM_012295281.1 Latest version!	Megachile rotundata cullin-4A (LOC100875727), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100875727 ( XM_003707602.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003707650.1 Megachile rotundata cullin-4A (LOC100875727), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe113403
Clone ID Related Accession (Same CDS sequence)	XM_003707602.2
Accession Version	XM_003707602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	cullin-4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797585.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGC TCGGAGGGAC TCAGGTTCTG GCCACAGCAC AGGTGAAGAG GACGGCCGCT 
GATCATACAG CGGTGTTAAA CTTTAGTGCT GCAAAACGCA GCAAACTATC CGCCGTTGCG 
GTCACGGAGA TCAACGAAAC TGAAAACATG ACGGATACAG CTGCGGCCAC AACTGATACT 
CAGAAGCGAG CCAATTTCTC AGCGTTAACC GTTGGGAATC CAAACGGCGT CAACAAGATT 
TCACCGAGTT TGGCAAGTGC AAAACCAGGC ACAGCTAGAA AGCTCGTCAT CAAAAATTTC 
AAAAATAAAC CAAAATTACC AGAAAATTAT CAAGAACAAA CATGGGAAAA GTTACAAGAA 
GCTGTAATTG CAATACAAAC CAGTAAAAGT ATTCGTTATT CATTAGAAGA ACTTTACCAA 
GCAGTTGAAA ATATGTGTAA TCATAAAATG GCATCTACGT TATATACCAA ATTGACAAGG 
TTAACAGAAG CTCATGTGCA GGCTAACATA GAGCAGTTCT TAGCAGAATC AATGGATAGG 
CATATATTCC TGAAAAAAAT GAATGAGTGT TGGCAGTCAC ATTGCAGACA AATGATCATG 
ATTAGAAGTA TTTTTCTATA TTTGGATAGA ACATATGTAT TACAAAACCC AAGTATTTTA 
TCTATTTGGG ACATGGGATT ACATTTGTTT AGGGTGTATA TTGTTCTAAA TAATTTAGTT 
CAAACACGTA CAGTAGAAGG ACTACTTATG CTCATAGAAA AAGAACGTCA GGGCGATACA 
GTAGACAGAA CGCTTCTTAA ATCATTATTA AGAATGTTAT CCGACCTACA AATCTACCAA 
GATGCCTTTG AATCCAAATT TTTGGTTGCT ACAGAAAGAT TATATGCTGC AGAAGGTCAA 
CGATTAATGA ACGAACACGA TGTTCCTGAG TACTTAGCAC ACGTAGATAA ACGTTTGCAG 
GAAGAAAATG AACGTTTATT ACACTATCTT GATGCATCTA CAAAGTGCTC GCTAATACAT 
ACAGTAGAAA AGCAATTATT ATCTGAACAT ATTACTAGCA TTTTACAAAA AGGATTGAGT 
GGTTTATTGG ATGAAAATAG AATTAGTGAT TTATCTTTAC TTTATAATTT ATATAGTCGT 
ATAAAGTGTG GTCTTATAGA ACTTTGTCAA AATTTCAATT CTTACATAAA GAAAAAGGGT 
AAAACTATAG TCATAGATCC AGAAAAAGAT AAAACTATGG TTCAAGAGCT TTTAGATTTC 
AAAGACAAAA TGGACAATAT AGTTAATACA TGCTTTCATA AAAATGAGAA ATTTGCTAAT 
AGTTTAAAAG AAGCTTTTGA AGCTTTTATT AACCAACGAG CAAATAAACC AGCGGAATTA 
ATAGCAAAAT TCGTTGATTG CAAATTGCGA GCTGGTAACA AGGAAGCAAC AGAAGAAGAA 
TTAGAAAGAT TATTAGATAA AATCATGGTA TTATTTCGAT TTATACATGG AAAAGATGTA 
TTCGAAGCAT TTTATAAAAA AGATTTGGCT AAACGTTTAT TAGTTGGTAA ATCAGCTTCT 
GTTGATGCTG AAAAATCCAT GTTATCGAAA TTAAAACAAG AATGTGGTGG TGGTTTCACT 
AGTAAATTGG AAGGAATGTT CAAAGATATG GAACTTAGCA AAGATATTAA TATTGCTTTT 
AAGCAGTATG CTGGAAATTT GCAAAGTGAA TTGTCTGCCA GCAATTTGGA TTTAACAGTT 
TCAATACTTA CAATGGGTTA TTGGCCAACA TATCCAGTAA TGGAAGTAAC ATTACCTCCA 
GAAATGGTTC AATACCAGGA TGTATTTAAT AAATTCTATT TGGGAAAGCA TAGTGGTAGA 
AAATTGCAGT GGCAACCTAC TTTGGGACAT TGTGTACTCA AAGCTTGGTT TAATCAGGGT 
AATAAAGAGT TTTTAGTGTC ATTATTTCAA GCATTGGTGT TACTTCTGTT CAATGACGCT 
GATAATTTAT CGCTGGAAGA TATAAAAGCA GCTACTAATA TAGAAGATGG TGAACTCAGA 
AGAACTTTAC AATCTTTAGC TTGCGGAAAA GCTAGAGTAT TACAAAAGAA CCCAAGAGGA 
AGAGATGTTG GGGATACCGA TAGATTTGTA TTTAATGCAG ATTTCACAAA TAAATTATTT 
AGAATAAAAA TAAATCAAAT TCAAATGAAA GAAACGAATG AAGAACAGAA GGCTACAGAA 
GAAAGGGTCT ATCAGGATAG ACAATATCAA ATAGATGCAG CTATTGTCAG AACTATGAAA 
ATGAGAAAAA CACTTACACA CAATTTATTA ATCAGTGAAC TGTATAATCA GTTAAAATTT 
CCTGTAAAGC CGGCTGATCT GAAGAAACGT ATTGAATCTC TTATAGATAG GGATTATATG 
GAACGAGATA AAGATAATGC AAATGAATAT AACTATGTTG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003707650.1
CDS	250..2694
Translation	MSRLGGTQVLATAQVKRTAADHTAVLNFSAAKRSKLSAVAVTEINETENMTDTAAATTDTQKRANFSALTVGNPNGVNKISPSLASAKPGTARKLVIKNFKNKPKLPENYQEQTWEKLQEAVIAIQTSKSIRYSLEELYQAVENMCNHKMASTLYTKLTRLTEAHVQANIEQFLAESMDRHIFLKKMNECWQSHCRQMIMIRSIFLYLDRTYVLQNPSILSIWDMGLHLFRVYIVLNNLVQTRTVEGLLMLIEKERQGDTVDRTLLKSLLRMLSDLQIYQDAFESKFLVATERLYAAEGQRLMNEHDVPEYLAHVDKRLQEENERLLHYLDASTKCSLIHTVEKQLLSEHITSILQKGLSGLLDENRISDLSLLYNLYSRIKCGLIELCQNFNSYIKKKGKTIVIDPEKDKTMVQELLDFKDKMDNIVNTCFHKNEKFANSLKEAFEAFINQRANKPAELIAKFVDCKLRAGNKEATEEELERLLDKIMVLFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTSKLEGMFKDMELSKDINIAFKQYAGNLQSELSASNLDLTVSILTMGYWPTYPVMEVTLPPEMVQYQDVFNKFYLGKHSGRKLQWQPTLGHCVLKAWFNQGNKEFLVSLFQALVLLLFNDADNLSLEDIKAATNIEDGELRRTLQSLACGKARVLQKNPRGRDVGDTDRFVFNADFTNKLFRIKINQIQMKETNEEQKATEERVYQDRQYQIDAAIVRTMKMRKTLTHNLLISELYNQLKFPVKPADLKKRIESLIDRDYMERDKDNANEYNYVA

Target ORF information:

RefSeq Version	XM_003707602.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata cullin-4A (LOC100875727), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707602.2

ORF Insert Sequence:

ATGTCTAGGC TCGGAGGGAC TCAGGTTCTG GCCACAGCAC AGGTGAAGAG GACGGCCGCT 
GATCATACAG CGGTGTTAAA CTTTAGTGCT GCAAAACGCA GCAAACTATC CGCCGTTGCG 
GTCACGGAGA TCAACGAAAC TGAAAACATG ACGGATACAG CTGCGGCCAC AACTGATACT 
CAGAAGCGAG CCAATTTCTC AGCGTTAACC GTTGGGAATC CAAACGGCGT CAACAAGATT 
TCACCGAGTT TGGCAAGTGC AAAACCAGGC ACAGCTAGAA AGCTCGTCAT CAAAAATTTC 
AAAAATAAAC CAAAATTACC AGAAAATTAT CAAGAACAAA CATGGGAAAA GTTACAAGAA 
GCTGTAATTG CAATACAAAC CAGTAAAAGT ATTCGTTATT CATTAGAAGA ACTTTACCAA 
GCAGTTGAAA ATATGTGTAA TCATAAAATG GCATCTACGT TATATACCAA ATTGACAAGG 
TTAACAGAAG CTCATGTGCA GGCTAACATA GAGCAGTTCT TAGCAGAATC AATGGATAGG 
CATATATTCC TGAAAAAAAT GAATGAGTGT TGGCAGTCAC ATTGCAGACA AATGATCATG 
ATTAGAAGTA TTTTTCTATA TTTGGATAGA ACATATGTAT TACAAAACCC AAGTATTTTA 
TCTATTTGGG ACATGGGATT ACATTTGTTT AGGGTGTATA TTGTTCTAAA TAATTTAGTT 
CAAACACGTA CAGTAGAAGG ACTACTTATG CTCATAGAAA AAGAACGTCA GGGCGATACA 
GTAGACAGAA CGCTTCTTAA ATCATTATTA AGAATGTTAT CCGACCTACA AATCTACCAA 
GATGCCTTTG AATCCAAATT TTTGGTTGCT ACAGAAAGAT TATATGCTGC AGAAGGTCAA 
CGATTAATGA ACGAACACGA TGTTCCTGAG TACTTAGCAC ACGTAGATAA ACGTTTGCAG 
GAAGAAAATG AACGTTTATT ACACTATCTT GATGCATCTA CAAAGTGCTC GCTAATACAT 
ACAGTAGAAA AGCAATTATT ATCTGAACAT ATTACTAGCA TTTTACAAAA AGGATTGAGT 
GGTTTATTGG ATGAAAATAG AATTAGTGAT TTATCTTTAC TTTATAATTT ATATAGTCGT 
ATAAAGTGTG GTCTTATAGA ACTTTGTCAA AATTTCAATT CTTACATAAA GAAAAAGGGT 
AAAACTATAG TCATAGATCC AGAAAAAGAT AAAACTATGG TTCAAGAGCT TTTAGATTTC 
AAAGACAAAA TGGACAATAT AGTTAATACA TGCTTTCATA AAAATGAGAA ATTTGCTAAT 
AGTTTAAAAG AAGCTTTTGA AGCTTTTATT AACCAACGAG CAAATAAACC AGCGGAATTA 
ATAGCAAAAT TCGTTGATTG CAAATTGCGA GCTGGTAACA AGGAAGCAAC AGAAGAAGAA 
TTAGAAAGAT TATTAGATAA AATCATGGTA TTATTTCGAT TTATACATGG AAAAGATGTA 
TTCGAAGCAT TTTATAAAAA AGATTTGGCT AAACGTTTAT TAGTTGGTAA ATCAGCTTCT 
GTTGATGCTG AAAAATCCAT GTTATCGAAA TTAAAACAAG AATGTGGTGG TGGTTTCACT 
AGTAAATTGG AAGGAATGTT CAAAGATATG GAACTTAGCA AAGATATTAA TATTGCTTTT 
AAGCAGTATG CTGGAAATTT GCAAAGTGAA TTGTCTGCCA GCAATTTGGA TTTAACAGTT 
TCAATACTTA CAATGGGTTA TTGGCCAACA TATCCAGTAA TGGAAGTAAC ATTACCTCCA 
GAAATGGTTC AATACCAGGA TGTATTTAAT AAATTCTATT TGGGAAAGCA TAGTGGTAGA 
AAATTGCAGT GGCAACCTAC TTTGGGACAT TGTGTACTCA AAGCTTGGTT TAATCAGGGT 
AATAAAGAGT TTTTAGTGTC ATTATTTCAA GCATTGGTGT TACTTCTGTT CAATGACGCT 
GATAATTTAT CGCTGGAAGA TATAAAAGCA GCTACTAATA TAGAAGATGG TGAACTCAGA 
AGAACTTTAC AATCTTTAGC TTGCGGAAAA GCTAGAGTAT TACAAAAGAA CCCAAGAGGA 
AGAGATGTTG GGGATACCGA TAGATTTGTA TTTAATGCAG ATTTCACAAA TAAATTATTT 
AGAATAAAAA TAAATCAAAT TCAAATGAAA GAAACGAATG AAGAACAGAA GGCTACAGAA 
GAAAGGGTCT ATCAGGATAG ACAATATCAA ATAGATGCAG CTATTGTCAG AACTATGAAA 
ATGAGAAAAA CACTTACACA CAATTTATTA ATCAGTGAAC TGTATAATCA GTTAAAATTT 
CCTGTAAAGC CGGCTGATCT GAAGAAACGT ATTGAATCTC TTATAGATAG GGATTATATG 
GAACGAGATA AAGATAATGC AAATGAATAT AACTATGTTG CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100875727 ( XM_012295281.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012150671.1 Megachile rotundata cullin-4A (LOC100875727), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe113404
Clone ID Related Accession (Same CDS sequence)	XM_012295281.1
Accession Version	XM_012295281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	cullin-4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797585.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGC TCGGAGGGAC TCAGGTTCTG GCCACAGCAC AGGTGAAGAG GACGGCCGCT 
GATCATACAG CGGTGTTAAA CTTTAGTGCT GCAAAACGCA GCAAACTATC CGCCGTTGCG 
GTCACGGAGA TCAACGAAAC TGAAAACATG ACGGATACAG CTGCGGCCAC AACTGATACT 
CAGAAGCGAG CCAATTTCTC AGCGTTAACC GTTGGGAATC CAAACGGCGT CAACAAGATT 
TCACCGAGTT TGGCAAGTGC AAAACCAGGC ACAGCTAGAA AGCTCGTCAT CAAAAATTTC 
AAAAATAAAC CAAAATTACC AGAAAATTAT CAAGAACAAA CATGGGAAAA GTTACAAGAA 
GCTGTAATTG CAATACAAAC CAGTAAAAGT ATTCGTTATT CATTAGAAGA ACTTTACCAA 
GCAGTTGAAA ATATGTGTAA TCATAAAATG GCATCTACGT TATATACCAA ATTGACAAGG 
TTAACAGAAG CTCATGTGCA GGCTAACATA GAGCAGTTCT TAGCAGAATC AATGGATAGG 
CATATATTCC TGAAAAAAAT GAATGAGTGT TGGCAGTCAC ATTGCAGACA AATGATCATG 
ATTAGAAGTA TTTTTCTATA TTTGGATAGA ACATATGTAT TACAAAACCC AAGTATTTTA 
TCTATTTGGG ACATGGGATT ACATTTGTTT AGGGTGTATA TTGTTCTAAA TAATTTAGTT 
CAAACACGTA CAGTAGAAGG ACTACTTATG CTCATAGAAA AAGAACGTCA GGGCGATACA 
GTAGACAGAA CGCTTCTTAA ATCATTATTA AGAATGTTAT CCGACCTACA AATCTACCAA 
GATGCCTTTG AATCCAAATT TTTGGTTGCT ACAGAAAGAT TATATGCTGC AGAAGGTCAA 
CGATTAATGA ACGAACACGA TGTTCCTGAG TACTTAGCAC ACGTAGATAA ACGTTTGCAG 
GAAGAAAATG AACGTTTATT ACACTATCTT GATGCATCTA CAAAGTGCTC GCTAATACAT 
ACAGTAGAAA AGCAATTATT ATCTGAACAT ATTACTAGCA TTTTACAAAA AGGATTGAGT 
GGTTTATTGG ATGAAAATAG AATTAGTGAT TTATCTTTAC TTTATAATTT ATATAGTCGT 
ATAAAGTGTG GTCTTATAGA ACTTTGTCAA AATTTCAATT CTTACATAAA GAAAAAGGGT 
AAAACTATAG TCATAGATCC AGAAAAAGAT AAAACTATGG TTCAAGAGCT TTTAGATTTC 
AAAGACAAAA TGGACAATAT AGTTAATACA TGCTTTCATA AAAATGAGAA ATTTGCTAAT 
AGTTTAAAAG AAGCTTTTGA AGCTTTTATT AACCAACGAG CAAATAAACC AGCGGAATTA 
ATAGCAAAAT TCGTTGATTG CAAATTGCGA GCTGGTAACA AGGAAGCAAC AGAAGAAGAA 
TTAGAAAGAT TATTAGATAA AATCATGGTA TTATTTCGAT TTATACATGG AAAAGATGTA 
TTCGAAGCAT TTTATAAAAA AGATTTGGCT AAACGTTTAT TAGTTGGTAA ATCAGCTTCT 
GTTGATGCTG AAAAATCCAT GTTATCGAAA TTAAAACAAG AATGTGGTGG TGGTTTCACT 
AGTAAATTGG AAGGAATGTT CAAAGATATG GAACTTAGCA AAGATATTAA TATTGCTTTT 
AAGCAGTATG CTGGAAATTT GCAAAGTGAA TTGTCTGCCA GCAATTTGGA TTTAACAGTT 
TCAATACTTA CAATGGGTTA TTGGCCAACA TATCCAGTAA TGGAAGTAAC ATTACCTCCA 
GAAATGGTTC AATACCAGGA TGTATTTAAT AAATTCTATT TGGGAAAGCA TAGTGGTAGA 
AAATTGCAGT GGCAACCTAC TTTGGGACAT TGTGTACTCA AAGCTTGGTT TAATCAGTGT 
CATTATTTCA AGCATTGGTG TTACTTCTGT TCAATGACGC TGATAATTTA TCGCTGGAAG 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012150671.1
CDS	259..2244
Translation	MSRLGGTQVLATAQVKRTAADHTAVLNFSAAKRSKLSAVAVTEINETENMTDTAAATTDTQKRANFSALTVGNPNGVNKISPSLASAKPGTARKLVIKNFKNKPKLPENYQEQTWEKLQEAVIAIQTSKSIRYSLEELYQAVENMCNHKMASTLYTKLTRLTEAHVQANIEQFLAESMDRHIFLKKMNECWQSHCRQMIMIRSIFLYLDRTYVLQNPSILSIWDMGLHLFRVYIVLNNLVQTRTVEGLLMLIEKERQGDTVDRTLLKSLLRMLSDLQIYQDAFESKFLVATERLYAAEGQRLMNEHDVPEYLAHVDKRLQEENERLLHYLDASTKCSLIHTVEKQLLSEHITSILQKGLSGLLDENRISDLSLLYNLYSRIKCGLIELCQNFNSYIKKKGKTIVIDPEKDKTMVQELLDFKDKMDNIVNTCFHKNEKFANSLKEAFEAFINQRANKPAELIAKFVDCKLRAGNKEATEEELERLLDKIMVLFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTSKLEGMFKDMELSKDINIAFKQYAGNLQSELSASNLDLTVSILTMGYWPTYPVMEVTLPPEMVQYQDVFNKFYLGKHSGRKLQWQPTLGHCVLKAWFNQCHYFKHWCYFCSMTLIIYRWKI

Target ORF information:

RefSeq Version	XM_012295281.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata cullin-4A (LOC100875727), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295281.1

ORF Insert Sequence:

ATGTCTAGGC TCGGAGGGAC TCAGGTTCTG GCCACAGCAC AGGTGAAGAG GACGGCCGCT 
GATCATACAG CGGTGTTAAA CTTTAGTGCT GCAAAACGCA GCAAACTATC CGCCGTTGCG 
GTCACGGAGA TCAACGAAAC TGAAAACATG ACGGATACAG CTGCGGCCAC AACTGATACT 
CAGAAGCGAG CCAATTTCTC AGCGTTAACC GTTGGGAATC CAAACGGCGT CAACAAGATT 
TCACCGAGTT TGGCAAGTGC AAAACCAGGC ACAGCTAGAA AGCTCGTCAT CAAAAATTTC 
AAAAATAAAC CAAAATTACC AGAAAATTAT CAAGAACAAA CATGGGAAAA GTTACAAGAA 
GCTGTAATTG CAATACAAAC CAGTAAAAGT ATTCGTTATT CATTAGAAGA ACTTTACCAA 
GCAGTTGAAA ATATGTGTAA TCATAAAATG GCATCTACGT TATATACCAA ATTGACAAGG 
TTAACAGAAG CTCATGTGCA GGCTAACATA GAGCAGTTCT TAGCAGAATC AATGGATAGG 
CATATATTCC TGAAAAAAAT GAATGAGTGT TGGCAGTCAC ATTGCAGACA AATGATCATG 
ATTAGAAGTA TTTTTCTATA TTTGGATAGA ACATATGTAT TACAAAACCC AAGTATTTTA 
TCTATTTGGG ACATGGGATT ACATTTGTTT AGGGTGTATA TTGTTCTAAA TAATTTAGTT 
CAAACACGTA CAGTAGAAGG ACTACTTATG CTCATAGAAA AAGAACGTCA GGGCGATACA 
GTAGACAGAA CGCTTCTTAA ATCATTATTA AGAATGTTAT CCGACCTACA AATCTACCAA 
GATGCCTTTG AATCCAAATT TTTGGTTGCT ACAGAAAGAT TATATGCTGC AGAAGGTCAA 
CGATTAATGA ACGAACACGA TGTTCCTGAG TACTTAGCAC ACGTAGATAA ACGTTTGCAG 
GAAGAAAATG AACGTTTATT ACACTATCTT GATGCATCTA CAAAGTGCTC GCTAATACAT 
ACAGTAGAAA AGCAATTATT ATCTGAACAT ATTACTAGCA TTTTACAAAA AGGATTGAGT 
GGTTTATTGG ATGAAAATAG AATTAGTGAT TTATCTTTAC TTTATAATTT ATATAGTCGT 
ATAAAGTGTG GTCTTATAGA ACTTTGTCAA AATTTCAATT CTTACATAAA GAAAAAGGGT 
AAAACTATAG TCATAGATCC AGAAAAAGAT AAAACTATGG TTCAAGAGCT TTTAGATTTC 
AAAGACAAAA TGGACAATAT AGTTAATACA TGCTTTCATA AAAATGAGAA ATTTGCTAAT 
AGTTTAAAAG AAGCTTTTGA AGCTTTTATT AACCAACGAG CAAATAAACC AGCGGAATTA 
ATAGCAAAAT TCGTTGATTG CAAATTGCGA GCTGGTAACA AGGAAGCAAC AGAAGAAGAA 
TTAGAAAGAT TATTAGATAA AATCATGGTA TTATTTCGAT TTATACATGG AAAAGATGTA 
TTCGAAGCAT TTTATAAAAA AGATTTGGCT AAACGTTTAT TAGTTGGTAA ATCAGCTTCT 
GTTGATGCTG AAAAATCCAT GTTATCGAAA TTAAAACAAG AATGTGGTGG TGGTTTCACT 
AGTAAATTGG AAGGAATGTT CAAAGATATG GAACTTAGCA AAGATATTAA TATTGCTTTT 
AAGCAGTATG CTGGAAATTT GCAAAGTGAA TTGTCTGCCA GCAATTTGGA TTTAACAGTT 
TCAATACTTA CAATGGGTTA TTGGCCAACA TATCCAGTAA TGGAAGTAAC ATTACCTCCA 
GAAATGGTTC AATACCAGGA TGTATTTAAT AAATTCTATT TGGGAAAGCA TAGTGGTAGA 
AAATTGCAGT GGCAACCTAC TTTGGGACAT TGTGTACTCA AAGCTTGGTT TAATCAGTGT 
CATTATTTCA AGCATTGGTG TTACTTCTGT TCAATGACGC TGATAATTTA TCGCTGGAAG 
ATATAA