MTR_4g066170 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_4g066170
Entrez Gene ID	25492621
Full Name	linoleate 13S-lipoxygenase 2-1, related protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013600788.2	XP_013456242.1	linoleate 13S-lipoxygenase 2-1, chloroplastic
XM_013600788.1	XP_013456242.1	linoleate 13S-lipoxygenase 2-1, related protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_4g066170 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_4g066170 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe188313	XM_013600788.2 Latest version!	Medicago truncatula linoleate 13S-lipoxygenase 2-1, chloroplastic (LOC25492621), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe188313	XM_013600788.1	Medicago truncatula linoleate 13S-lipoxygenase 2-1, related protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_4g066170 ( XM_013600788.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013456242.1 Medicago truncatula linoleate 13S-lipoxygenase 2-1, chloroplastic (LOC25492621), mRNA.

Price & Availability↑

CloneID	OMe188313
Clone ID Related Accession (Same CDS sequence)	XM_013600788.2 , XM_013600788.1
Accession Version	XM_013600788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	linoleate 13S-lipoxygenase 2-1, chloroplastic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013600788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATGC AACAAATTAG TTTAAAGCCA AATTGTCTCA TCTTGAACAA CCCATGTCTC 
CATGGTATTG GAAGTAACCA AACATGTTTT CATGTTGGAT CGTCAAGGCC TTTGTTCACA 
AACCAAAAGA AACAAAGGAG GTTGTTTAAG AGGAATGAGT GTAGCAAAAT AAAAGCTGTG 
GCTGTGAGTG ATGAAGAACT TATAGAACAA ACATCATTAA AAGTAAAAGC CATAGTAACT 
GTTCAACCAA CAGTTGGAGG GCTTTTCTCA GAAATGGCTC TAGAAAGAGG GCTTGATGAC 
ATAACAGATT TGTTGGGTAA ATCAATTCTC TTGGAGTTTG TTAGCTCCGA GCTGGATCCC 
GAGACAAAAT TGGAGAAGGA AAGAATCAAA GGTTACGTCC ACCAAACGCA TAGATCAGTG 
GAAGAGATTA AATATGAAGC TGAATTTGAA GTACCACAAA GTTTTGGAGA TATTGGTGCA 
GTTCTTGTGG AAAATGAGCA TAGAAGGGAA ATGTTTATAA AGAACATTGT TCTTGATGGC 
TTTCTCACTG GTCCTGTGAA TTTTTCTTGT GAATCATGGG TTCATTCCAA GCATGACAAC 
CCTGATGATA AGAGGGTTAT TTTTTCCAAC AAGTCATATT TACCATCTGA AACACCAGAA 
GGATTGATAA GGTTAAGAGA AGAGGAACTG AAAACTTTAC GAGGCAATGG ACAAGGCGAA 
CGCAAGAGCT TTGATCGAAT ATACGATTAC GATGTGTATA ATGACCTCGG TGATCCAGAC 
AGCGATCCCG AACTCAAGCG ACCTGTTCTT GGTGGCAAAG AACATCCATA TCCTAGGCGT 
TGTAGAACCG GAAGACCTCG TTGTGATACA GATCCGTTGT CTGAGAAAAG AAGTAACAAT 
GTCTATGTTC CGAGGGACGA ATCCTTCTCA GAATTAAAGC AACTCTCGTT TTCAGCAAAT 
ACCTTACAAT CGGCTTTCCA TGCGCTTTTA CCGGTCGTGA AAACAGCTGT AATTGACAAG 
AATCTTGGAT TCCCTCTTTT CTCAGCCATA GATGACCTTT TCAATGAAGG ATTTAACTTG 
CCTCCTCAAG CTGAAAAAGG GTTTCTTGCG TCTGTGTTGC CAAGGTTGGT CAGGCTTGTC 
AACGAGGCTA GGAATGACAT TCTCCGTTTC GAGACTCCCG CCACAATGGA CAAGGACAGA 
TTCTTTTGGT TTAGGGATGA AGAATTTGGA AGACAGACTA TAGCAGGTCT TAATCCTTGT 
TGCATCCAAT TGGTCACGGA ATGGCCATTG AAAAGCAAAC TTGACCCTAA AATTTATGGT 
CCTGCTGAAT CTGCAATAAC CACTGAAATG ATTGAGCAAC AAATCAGAGG ATATGCTACC 
GTTGAAGAGG CGATAAAACA GAAGAAGTTG TTCATCCTAG ACTACTACGA TTTTTTCTTG 
CCGTTGGTCG AGGAAGTTAG AAAACTCGAA GGCACAACAT TGTATGGATC AAGGACGTTG 
TTCTTTCTGA ATGAAGATAG CACATTGAGA CCGGTGGCGA TCGAGCTAGC TAGGCCGCCA 
ATTGGCGGAA AGCCAGAATG GAGGGAAGTC TTCACACCTG CTTGGCATTC AACTGGTGTT 
TGGCTTTGGA GGCTTGCCAA AGCTCATGTC CTTGCTCATG ACTCTGGCTA CCATCAACTT 
GTTAGTCACT GGTTGAGAAC TCATTGTGCC ACAGAGCCTT ACATCATAGC AACAAACAGA 
CAACTAAGTG CAATGCATCC AATCTACAAA CTATTACTTC CACATTTTCG ATACACAATG 
GAAATCAATG CACTTGCACG CGAAGCACTT ATAAATGCAG ATGGAATAAT TGAGAGTAGT 
TTCACTCCTA AACAACTCTC TCTCTTGGTA AGTTCAATAG CCTATGATAA GCATTGGCAA 
TTTGACTTGC AAGCCCTTCC AAATGACCTC ATCCATAGAG GACTAGCAGA AAAAGATCCA 
AATGCCCCAC ATGGCCTAAA ACTAGCAATA GAAGATTACC CTTATGCAAA TGATGGTCTT 
GTTCTTTGGG ATACGATCAA ATCTTGGGTC ACTGATTATG TTAACCATTA CTACAACAAT 
GAGTCGAGAA ATGTTGAGTC GGATAAAGAA TTACAAGCAT GGTGGGATGA GATTATAAAT 
GTTGGTCACG GCGACAAAAA AGACGAACCA TGGTGGCCTA ATTTGAAAAC AAACGATGAT 
CTTGTTGAAA TAGTGACGAC CATTGTTTGG ATTACATCGG GGCATCATGC TGCGGTGAAT 
TTTGGTCAGT ATACTTATGC TGGATATTTT CCTAATAGAC CGGCCATTGC GCGAAACAAC 
ATGCCTACAG AAGACCCTTC AGACAGCGAA TGGGAACTTT TCTTCGAGAA ACCAGAAGTG 
ACATTGTTGA AGTGTTTCCC TTCACAAATT CAAGCAATGA CGGTGATGAC CGTGTTGGAT 
ATTTTGTCAA GTCATTCGCC GGATGAGGAA TATCTTGGAG AAACGATTGA ACCGGCATGG 
CAAGAGGAAC CAATGATTAA AGCTGCTTTT GAAAAGTTTA AAGGGAGGTT GATGGAGCTT 
GAAGGTATAG TAGATGAGAG GAATGCTAAT ATGAATTTCA GGAATAGAAA TGGTGCTGGA 
ATTTTGCCTT ATGAGCTTTT GAAGCCAACA TCAGAGCCTG GTGTAACAGG AAAAGGTGTT 
CCATATAGTG TCTCTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013456242.1
CDS	112..2832
Translation	MLMQQISLKPNCLILNNPCLHGIGSNQTCFHVGSSRPLFTNQKKQRRLFKRNECSKIKAVAVSDEELIEQTSLKVKAIVTVQPTVGGLFSEMALERGLDDITDLLGKSILLEFVSSELDPETKLEKERIKGYVHQTHRSVEEIKYEAEFEVPQSFGDIGAVLVENEHRREMFIKNIVLDGFLTGPVNFSCESWVHSKHDNPDDKRVIFSNKSYLPSETPEGLIRLREEELKTLRGNGQGERKSFDRIYDYDVYNDLGDPDSDPELKRPVLGGKEHPYPRRCRTGRPRCDTDPLSEKRSNNVYVPRDESFSELKQLSFSANTLQSAFHALLPVVKTAVIDKNLGFPLFSAIDDLFNEGFNLPPQAEKGFLASVLPRLVRLVNEARNDILRFETPATMDKDRFFWFRDEEFGRQTIAGLNPCCIQLVTEWPLKSKLDPKIYGPAESAITTEMIEQQIRGYATVEEAIKQKKLFILDYYDFFLPLVEEVRKLEGTTLYGSRTLFFLNEDSTLRPVAIELARPPIGGKPEWREVFTPAWHSTGVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSAMHPIYKLLLPHFRYTMEINALAREALINADGIIESSFTPKQLSLLVSSIAYDKHWQFDLQALPNDLIHRGLAEKDPNAPHGLKLAIEDYPYANDGLVLWDTIKSWVTDYVNHYYNNESRNVESDKELQAWWDEIINVGHGDKKDEPWWPNLKTNDDLVEIVTTIVWITSGHHAAVNFGQYTYAGYFPNRPAIARNNMPTEDPSDSEWELFFEKPEVTLLKCFPSQIQAMTVMTVLDILSSHSPDEEYLGETIEPAWQEEPMIKAAFEKFKGRLMELEGIVDERNANMNFRNRNGAGILPYELLKPTSEPGVTGKGVPYSVSI

Target ORF information:

RefSeq Version	XM_013600788.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula linoleate 13S-lipoxygenase 2-1, chloroplastic (LOC25492621), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013600788.2

ORF Insert Sequence:

ATGCTCATGC AACAAATTAG TTTAAAGCCA AATTGTCTCA TCTTGAACAA CCCATGTCTC 
CATGGTATTG GAAGTAACCA AACATGTTTT CATGTTGGAT CGTCAAGGCC TTTGTTCACA 
AACCAAAAGA AACAAAGGAG GTTGTTTAAG AGGAATGAGT GTAGCAAAAT AAAAGCTGTG 
GCTGTGAGTG ATGAAGAACT TATAGAACAA ACATCATTAA AAGTAAAAGC CATAGTAACT 
GTTCAACCAA CAGTTGGAGG GCTTTTCTCA GAAATGGCTC TAGAAAGAGG GCTTGATGAC 
ATAACAGATT TGTTGGGTAA ATCAATTCTC TTGGAGTTTG TTAGCTCCGA GCTGGATCCC 
GAGACAAAAT TGGAGAAGGA AAGAATCAAA GGTTACGTCC ACCAAACGCA TAGATCAGTG 
GAAGAGATTA AATATGAAGC TGAATTTGAA GTACCACAAA GTTTTGGAGA TATTGGTGCA 
GTTCTTGTGG AAAATGAGCA TAGAAGGGAA ATGTTTATAA AGAACATTGT TCTTGATGGC 
TTTCTCACTG GTCCTGTGAA TTTTTCTTGT GAATCATGGG TTCATTCCAA GCATGACAAC 
CCTGATGATA AGAGGGTTAT TTTTTCCAAC AAGTCATATT TACCATCTGA AACACCAGAA 
GGATTGATAA GGTTAAGAGA AGAGGAACTG AAAACTTTAC GAGGCAATGG ACAAGGCGAA 
CGCAAGAGCT TTGATCGAAT ATACGATTAC GATGTGTATA ATGACCTCGG TGATCCAGAC 
AGCGATCCCG AACTCAAGCG ACCTGTTCTT GGTGGCAAAG AACATCCATA TCCTAGGCGT 
TGTAGAACCG GAAGACCTCG TTGTGATACA GATCCGTTGT CTGAGAAAAG AAGTAACAAT 
GTCTATGTTC CGAGGGACGA ATCCTTCTCA GAATTAAAGC AACTCTCGTT TTCAGCAAAT 
ACCTTACAAT CGGCTTTCCA TGCGCTTTTA CCGGTCGTGA AAACAGCTGT AATTGACAAG 
AATCTTGGAT TCCCTCTTTT CTCAGCCATA GATGACCTTT TCAATGAAGG ATTTAACTTG 
CCTCCTCAAG CTGAAAAAGG GTTTCTTGCG TCTGTGTTGC CAAGGTTGGT CAGGCTTGTC 
AACGAGGCTA GGAATGACAT TCTCCGTTTC GAGACTCCCG CCACAATGGA CAAGGACAGA 
TTCTTTTGGT TTAGGGATGA AGAATTTGGA AGACAGACTA TAGCAGGTCT TAATCCTTGT 
TGCATCCAAT TGGTCACGGA ATGGCCATTG AAAAGCAAAC TTGACCCTAA AATTTATGGT 
CCTGCTGAAT CTGCAATAAC CACTGAAATG ATTGAGCAAC AAATCAGAGG ATATGCTACC 
GTTGAAGAGG CGATAAAACA GAAGAAGTTG TTCATCCTAG ACTACTACGA TTTTTTCTTG 
CCGTTGGTCG AGGAAGTTAG AAAACTCGAA GGCACAACAT TGTATGGATC AAGGACGTTG 
TTCTTTCTGA ATGAAGATAG CACATTGAGA CCGGTGGCGA TCGAGCTAGC TAGGCCGCCA 
ATTGGCGGAA AGCCAGAATG GAGGGAAGTC TTCACACCTG CTTGGCATTC AACTGGTGTT 
TGGCTTTGGA GGCTTGCCAA AGCTCATGTC CTTGCTCATG ACTCTGGCTA CCATCAACTT 
GTTAGTCACT GGTTGAGAAC TCATTGTGCC ACAGAGCCTT ACATCATAGC AACAAACAGA 
CAACTAAGTG CAATGCATCC AATCTACAAA CTATTACTTC CACATTTTCG ATACACAATG 
GAAATCAATG CACTTGCACG CGAAGCACTT ATAAATGCAG ATGGAATAAT TGAGAGTAGT 
TTCACTCCTA AACAACTCTC TCTCTTGGTA AGTTCAATAG CCTATGATAA GCATTGGCAA 
TTTGACTTGC AAGCCCTTCC AAATGACCTC ATCCATAGAG GACTAGCAGA AAAAGATCCA 
AATGCCCCAC ATGGCCTAAA ACTAGCAATA GAAGATTACC CTTATGCAAA TGATGGTCTT 
GTTCTTTGGG ATACGATCAA ATCTTGGGTC ACTGATTATG TTAACCATTA CTACAACAAT 
GAGTCGAGAA ATGTTGAGTC GGATAAAGAA TTACAAGCAT GGTGGGATGA GATTATAAAT 
GTTGGTCACG GCGACAAAAA AGACGAACCA TGGTGGCCTA ATTTGAAAAC AAACGATGAT 
CTTGTTGAAA TAGTGACGAC CATTGTTTGG ATTACATCGG GGCATCATGC TGCGGTGAAT 
TTTGGTCAGT ATACTTATGC TGGATATTTT CCTAATAGAC CGGCCATTGC GCGAAACAAC 
ATGCCTACAG AAGACCCTTC AGACAGCGAA TGGGAACTTT TCTTCGAGAA ACCAGAAGTG 
ACATTGTTGA AGTGTTTCCC TTCACAAATT CAAGCAATGA CGGTGATGAC CGTGTTGGAT 
ATTTTGTCAA GTCATTCGCC GGATGAGGAA TATCTTGGAG AAACGATTGA ACCGGCATGG 
CAAGAGGAAC CAATGATTAA AGCTGCTTTT GAAAAGTTTA AAGGGAGGTT GATGGAGCTT 
GAAGGTATAG TAGATGAGAG GAATGCTAAT ATGAATTTCA GGAATAGAAA TGGTGCTGGA 
ATTTTGCCTT ATGAGCTTTT GAAGCCAACA TCAGAGCCTG GTGTAACAGG AAAAGGTGTT 
CCATATAGTG TCTCTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_4g066170 ( XM_013600788.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013456242.1 Medicago truncatula linoleate 13S-lipoxygenase 2-1, related protein mRNA.

Price & Availability↑

CloneID	OMe188313
Clone ID Related Accession (Same CDS sequence)	XM_013600788.2 , XM_013600788.1
Accession Version	XM_013600788.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	linoleate 13S-lipoxygenase 2-1, related protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016410). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGCTCATGC AACAAATTAG TTTAAAGCCA AATTGTCTCA TCTTGAACAA CCCATGTCTC 
CATGGTATTG GAAGTAACCA AACATGTTTT CATGTTGGAT CGTCAAGGCC TTTGTTCACA 
AACCAAAAGA AACAAAGGAG GTTGTTTAAG AGGAATGAGT GTAGCAAAAT AAAAGCTGTG 
GCTGTGAGTG ATGAAGAACT TATAGAACAA ACATCATTAA AAGTAAAAGC CATAGTAACT 
GTTCAACCAA CAGTTGGAGG GCTTTTCTCA GAAATGGCTC TAGAAAGAGG GCTTGATGAC 
ATAACAGATT TGTTGGGTAA ATCAATTCTC TTGGAGTTTG TTAGCTCCGA GCTGGATCCC 
GAGACAAAAT TGGAGAAGGA AAGAATCAAA GGTTACGTCC ACCAAACGCA TAGATCAGTG 
GAAGAGATTA AATATGAAGC TGAATTTGAA GTACCACAAA GTTTTGGAGA TATTGGTGCA 
GTTCTTGTGG AAAATGAGCA TAGAAGGGAA ATGTTTATAA AGAACATTGT TCTTGATGGC 
TTTCTCACTG GTCCTGTGAA TTTTTCTTGT GAATCATGGG TTCATTCCAA GCATGACAAC 
CCTGATGATA AGAGGGTTAT TTTTTCCAAC AAGTCATATT TACCATCTGA AACACCAGAA 
GGATTGATAA GGTTAAGAGA AGAGGAACTG AAAACTTTAC GAGGCAATGG ACAAGGCGAA 
CGCAAGAGCT TTGATCGAAT ATACGATTAC GATGTGTATA ATGACCTCGG TGATCCAGAC 
AGCGATCCCG AACTCAAGCG ACCTGTTCTT GGTGGCAAAG AACATCCATA TCCTAGGCGT 
TGTAGAACCG GAAGACCTCG TTGTGATACA GATCCGTTGT CTGAGAAAAG AAGTAACAAT 
GTCTATGTTC CGAGGGACGA ATCCTTCTCA GAATTAAAGC AACTCTCGTT TTCAGCAAAT 
ACCTTACAAT CGGCTTTCCA TGCGCTTTTA CCGGTCGTGA AAACAGCTGT AATTGACAAG 
AATCTTGGAT TCCCTCTTTT CTCAGCCATA GATGACCTTT TCAATGAAGG ATTTAACTTG 
CCTCCTCAAG CTGAAAAAGG GTTTCTTGCG TCTGTGTTGC CAAGGTTGGT CAGGCTTGTC 
AACGAGGCTA GGAATGACAT TCTCCGTTTC GAGACTCCCG CCACAATGGA CAAGGACAGA 
TTCTTTTGGT TTAGGGATGA AGAATTTGGA AGACAGACTA TAGCAGGTCT TAATCCTTGT 
TGCATCCAAT TGGTCACGGA ATGGCCATTG AAAAGCAAAC TTGACCCTAA AATTTATGGT 
CCTGCTGAAT CTGCAATAAC CACTGAAATG ATTGAGCAAC AAATCAGAGG ATATGCTACC 
GTTGAAGAGG CGATAAAACA GAAGAAGTTG TTCATCCTAG ACTACTACGA TTTTTTCTTG 
CCGTTGGTCG AGGAAGTTAG AAAACTCGAA GGCACAACAT TGTATGGATC AAGGACGTTG 
TTCTTTCTGA ATGAAGATAG CACATTGAGA CCGGTGGCGA TCGAGCTAGC TAGGCCGCCA 
ATTGGCGGAA AGCCAGAATG GAGGGAAGTC TTCACACCTG CTTGGCATTC AACTGGTGTT 
TGGCTTTGGA GGCTTGCCAA AGCTCATGTC CTTGCTCATG ACTCTGGCTA CCATCAACTT 
GTTAGTCACT GGTTGAGAAC TCATTGTGCC ACAGAGCCTT ACATCATAGC AACAAACAGA 
CAACTAAGTG CAATGCATCC AATCTACAAA CTATTACTTC CACATTTTCG ATACACAATG 
GAAATCAATG CACTTGCACG CGAAGCACTT ATAAATGCAG ATGGAATAAT TGAGAGTAGT 
TTCACTCCTA AACAACTCTC TCTCTTGGTA AGTTCAATAG CCTATGATAA GCATTGGCAA 
TTTGACTTGC AAGCCCTTCC AAATGACCTC ATCCATAGAG GACTAGCAGA AAAAGATCCA 
AATGCCCCAC ATGGCCTAAA ACTAGCAATA GAAGATTACC CTTATGCAAA TGATGGTCTT 
GTTCTTTGGG ATACGATCAA ATCTTGGGTC ACTGATTATG TTAACCATTA CTACAACAAT 
GAGTCGAGAA ATGTTGAGTC GGATAAAGAA TTACAAGCAT GGTGGGATGA GATTATAAAT 
GTTGGTCACG GCGACAAAAA AGACGAACCA TGGTGGCCTA ATTTGAAAAC AAACGATGAT 
CTTGTTGAAA TAGTGACGAC CATTGTTTGG ATTACATCGG GGCATCATGC TGCGGTGAAT 
TTTGGTCAGT ATACTTATGC TGGATATTTT CCTAATAGAC CGGCCATTGC GCGAAACAAC 
ATGCCTACAG AAGACCCTTC AGACAGCGAA TGGGAACTTT TCTTCGAGAA ACCAGAAGTG 
ACATTGTTGA AGTGTTTCCC TTCACAAATT CAAGCAATGA CGGTGATGAC CGTGTTGGAT 
ATTTTGTCAA GTCATTCGCC GGATGAGGAA TATCTTGGAG AAACGATTGA ACCGGCATGG 
CAAGAGGAAC CAATGATTAA AGCTGCTTTT GAAAAGTTTA AAGGGAGGTT GATGGAGCTT 
GAAGGTATAG TAGATGAGAG GAATGCTAAT ATGAATTTCA GGAATAGAAA TGGTGCTGGA 
ATTTTGCCTT ATGAGCTTTT GAAGCCAACA TCAGAGCCTG GTGTAACAGG AAAAGGTGTT 
CCATATAGTG TCTCTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013456242.1
CDS	88..2808
Translation	MLMQQISLKPNCLILNNPCLHGIGSNQTCFHVGSSRPLFTNQKKQRRLFKRNECSKIKAVAVSDEELIEQTSLKVKAIVTVQPTVGGLFSEMALERGLDDITDLLGKSILLEFVSSELDPETKLEKERIKGYVHQTHRSVEEIKYEAEFEVPQSFGDIGAVLVENEHRREMFIKNIVLDGFLTGPVNFSCESWVHSKHDNPDDKRVIFSNKSYLPSETPEGLIRLREEELKTLRGNGQGERKSFDRIYDYDVYNDLGDPDSDPELKRPVLGGKEHPYPRRCRTGRPRCDTDPLSEKRSNNVYVPRDESFSELKQLSFSANTLQSAFHALLPVVKTAVIDKNLGFPLFSAIDDLFNEGFNLPPQAEKGFLASVLPRLVRLVNEARNDILRFETPATMDKDRFFWFRDEEFGRQTIAGLNPCCIQLVTEWPLKSKLDPKIYGPAESAITTEMIEQQIRGYATVEEAIKQKKLFILDYYDFFLPLVEEVRKLEGTTLYGSRTLFFLNEDSTLRPVAIELARPPIGGKPEWREVFTPAWHSTGVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSAMHPIYKLLLPHFRYTMEINALAREALINADGIIESSFTPKQLSLLVSSIAYDKHWQFDLQALPNDLIHRGLAEKDPNAPHGLKLAIEDYPYANDGLVLWDTIKSWVTDYVNHYYNNESRNVESDKELQAWWDEIINVGHGDKKDEPWWPNLKTNDDLVEIVTTIVWITSGHHAAVNFGQYTYAGYFPNRPAIARNNMPTEDPSDSEWELFFEKPEVTLLKCFPSQIQAMTVMTVLDILSSHSPDEEYLGETIEPAWQEEPMIKAAFEKFKGRLMELEGIVDERNANMNFRNRNGAGILPYELLKPTSEPGVTGKGVPYSVSI

Target ORF information:

RefSeq Version	XM_013600788.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula linoleate 13S-lipoxygenase 2-1, related protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013600788.1

ORF Insert Sequence:

ATGCTCATGC AACAAATTAG TTTAAAGCCA AATTGTCTCA TCTTGAACAA CCCATGTCTC 
CATGGTATTG GAAGTAACCA AACATGTTTT CATGTTGGAT CGTCAAGGCC TTTGTTCACA 
AACCAAAAGA AACAAAGGAG GTTGTTTAAG AGGAATGAGT GTAGCAAAAT AAAAGCTGTG 
GCTGTGAGTG ATGAAGAACT TATAGAACAA ACATCATTAA AAGTAAAAGC CATAGTAACT 
GTTCAACCAA CAGTTGGAGG GCTTTTCTCA GAAATGGCTC TAGAAAGAGG GCTTGATGAC 
ATAACAGATT TGTTGGGTAA ATCAATTCTC TTGGAGTTTG TTAGCTCCGA GCTGGATCCC 
GAGACAAAAT TGGAGAAGGA AAGAATCAAA GGTTACGTCC ACCAAACGCA TAGATCAGTG 
GAAGAGATTA AATATGAAGC TGAATTTGAA GTACCACAAA GTTTTGGAGA TATTGGTGCA 
GTTCTTGTGG AAAATGAGCA TAGAAGGGAA ATGTTTATAA AGAACATTGT TCTTGATGGC 
TTTCTCACTG GTCCTGTGAA TTTTTCTTGT GAATCATGGG TTCATTCCAA GCATGACAAC 
CCTGATGATA AGAGGGTTAT TTTTTCCAAC AAGTCATATT TACCATCTGA AACACCAGAA 
GGATTGATAA GGTTAAGAGA AGAGGAACTG AAAACTTTAC GAGGCAATGG ACAAGGCGAA 
CGCAAGAGCT TTGATCGAAT ATACGATTAC GATGTGTATA ATGACCTCGG TGATCCAGAC 
AGCGATCCCG AACTCAAGCG ACCTGTTCTT GGTGGCAAAG AACATCCATA TCCTAGGCGT 
TGTAGAACCG GAAGACCTCG TTGTGATACA GATCCGTTGT CTGAGAAAAG AAGTAACAAT 
GTCTATGTTC CGAGGGACGA ATCCTTCTCA GAATTAAAGC AACTCTCGTT TTCAGCAAAT 
ACCTTACAAT CGGCTTTCCA TGCGCTTTTA CCGGTCGTGA AAACAGCTGT AATTGACAAG 
AATCTTGGAT TCCCTCTTTT CTCAGCCATA GATGACCTTT TCAATGAAGG ATTTAACTTG 
CCTCCTCAAG CTGAAAAAGG GTTTCTTGCG TCTGTGTTGC CAAGGTTGGT CAGGCTTGTC 
AACGAGGCTA GGAATGACAT TCTCCGTTTC GAGACTCCCG CCACAATGGA CAAGGACAGA 
TTCTTTTGGT TTAGGGATGA AGAATTTGGA AGACAGACTA TAGCAGGTCT TAATCCTTGT 
TGCATCCAAT TGGTCACGGA ATGGCCATTG AAAAGCAAAC TTGACCCTAA AATTTATGGT 
CCTGCTGAAT CTGCAATAAC CACTGAAATG ATTGAGCAAC AAATCAGAGG ATATGCTACC 
GTTGAAGAGG CGATAAAACA GAAGAAGTTG TTCATCCTAG ACTACTACGA TTTTTTCTTG 
CCGTTGGTCG AGGAAGTTAG AAAACTCGAA GGCACAACAT TGTATGGATC AAGGACGTTG 
TTCTTTCTGA ATGAAGATAG CACATTGAGA CCGGTGGCGA TCGAGCTAGC TAGGCCGCCA 
ATTGGCGGAA AGCCAGAATG GAGGGAAGTC TTCACACCTG CTTGGCATTC AACTGGTGTT 
TGGCTTTGGA GGCTTGCCAA AGCTCATGTC CTTGCTCATG ACTCTGGCTA CCATCAACTT 
GTTAGTCACT GGTTGAGAAC TCATTGTGCC ACAGAGCCTT ACATCATAGC AACAAACAGA 
CAACTAAGTG CAATGCATCC AATCTACAAA CTATTACTTC CACATTTTCG ATACACAATG 
GAAATCAATG CACTTGCACG CGAAGCACTT ATAAATGCAG ATGGAATAAT TGAGAGTAGT 
TTCACTCCTA AACAACTCTC TCTCTTGGTA AGTTCAATAG CCTATGATAA GCATTGGCAA 
TTTGACTTGC AAGCCCTTCC AAATGACCTC ATCCATAGAG GACTAGCAGA AAAAGATCCA 
AATGCCCCAC ATGGCCTAAA ACTAGCAATA GAAGATTACC CTTATGCAAA TGATGGTCTT 
GTTCTTTGGG ATACGATCAA ATCTTGGGTC ACTGATTATG TTAACCATTA CTACAACAAT 
GAGTCGAGAA ATGTTGAGTC GGATAAAGAA TTACAAGCAT GGTGGGATGA GATTATAAAT 
GTTGGTCACG GCGACAAAAA AGACGAACCA TGGTGGCCTA ATTTGAAAAC AAACGATGAT 
CTTGTTGAAA TAGTGACGAC CATTGTTTGG ATTACATCGG GGCATCATGC TGCGGTGAAT 
TTTGGTCAGT ATACTTATGC TGGATATTTT CCTAATAGAC CGGCCATTGC GCGAAACAAC 
ATGCCTACAG AAGACCCTTC AGACAGCGAA TGGGAACTTT TCTTCGAGAA ACCAGAAGTG 
ACATTGTTGA AGTGTTTCCC TTCACAAATT CAAGCAATGA CGGTGATGAC CGTGTTGGAT 
ATTTTGTCAA GTCATTCGCC GGATGAGGAA TATCTTGGAG AAACGATTGA ACCGGCATGG 
CAAGAGGAAC CAATGATTAA AGCTGCTTTT GAAAAGTTTA AAGGGAGGTT GATGGAGCTT 
GAAGGTATAG TAGATGAGAG GAATGCTAAT ATGAATTTCA GGAATAGAAA TGGTGCTGGA 
ATTTTGCCTT ATGAGCTTTT GAAGCCAACA TCAGAGCCTG GTGTAACAGG AAAAGGTGTT 
CCATATAGTG TCTCTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_4g066170 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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