LOC25489411 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25489411
Entrez Gene ID	25489411
Full Name	acetyl-CoA carboxylase
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013605390.1	XP_013460844.1	acetyl-CoA carboxylase
XM_013605390.2	XP_013460844.2	LOW QUALITY PROTEIN: acetyl-CoA carboxylase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25489411 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25489411 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe261073	XM_013605390.2 Latest version!	Medicago truncatula acetyl-CoA carboxylase 1 (LOC25489411), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25489411 ( XM_013605390.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013460844.2 Medicago truncatula acetyl-CoA carboxylase 1 (LOC25489411), mRNA.

Price & Availability↑

CloneID	OMe261073
Clone ID Related Accession (Same CDS sequence)	XM_013605390.2
Accession Version	XM_013605390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	LOW QUALITY PROTEIN: acetyl-CoA carboxylase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013605390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTAGCG TGGGTGAATA CTGCAATGCC CTTGGGGGAA ACATGCCAAT TCATAGTATT 
TTGATTGCAA ACAATGGATT GGCTGCAGTC AAGTTTATTC GCAGCGTTAG GAGTTGGGCT 
TATGAGACGT TCGGAACGGA AAATGCTATT TTCTTGGTTG CCATGGCTAC TCCAGAGGAC 
ATGAGAATCA ATGCAGAACA TATAAGAATT GCTAATCAGT TTGTGGAAGT ACCTGGTGGG 
ACCAATAACA ATAACTATGC CAATGTGCAA CTTATTCTAG AGATTGCTGA GATAACTCAC 
GTTGATGCGG TGTGGCCTGG TTGGGGTCAT GCATCAGAGG ATCTTGAGCT TCCAAATGCA 
TTGAAAGAAA AAGGAATTGT ATTTCTTGGA CCTCCTGCTA TATCTATGGC AGCTTTGGGA 
GATAAAATTG CTTCATTGTT GATTGCTCAA GCAGCAGAAG TGCCAACCCT TCCATGGAGT 
GGTTCTCATG TGAAATTTCC TCCAGAAAGC GACTTGATTA CTATTCCTGA TGAAATTTAC 
CGTGCATCAT GTGTTTATAC AACAGAAGAA GCAATTGCAA GTTGTCAAGT TGTAGGTTAC 
CCTGCAATGA TCAAGGCATC ATGGGGGGGC GGCGGTAAAG GCATAAGAAA GGTTCATAAT 
GATGACGAGG TAAGGGCATT GTTCAAGCAA GTTCAAGGTG AAGTTCCAGG CTCACCTATA 
TTTATAATGA AAGTTGCTTC CCAGAGCCGA CATCTTGAAG TCCAATTGAT TTGTGATCAG 
TATGGAAATG TTGCAGCTTT GCACAGCCGT GATTGCAGTG TTCAAAGAAG GCATCAAAAG 
ATTATTGAGG AAGGTCCCGT TACTGTAGCA CCTCCAGAAA CAGTGAAAGA ACTTGAACAT 
GCGGCTAGAA GATTAGCTAA ATCTGTAAAT TATGTTGGGG CAGCTACCGT TGAGTATCTT 
TATAGCATGG AAACTGGCGA GTACTACTTT TTAGAGTTGA ACCCACGACT ACAGGTTGAG 
CATCCTGTTA CTGAATGGAT AGCTGAGATA AATCTGCCAG CAGCACAAGT TGCAGTTGGG 
ATGGGTATCC CTCTCTGGAA AATTCCTGAG ATTAGGCGTT TCTATGGGAT GGAACATGGT 
GGGGGAAATG ATGCTTGGAG GAAAACATCA GTTTTAGGTA CCCCTTTTGA TTTTGACAAA 
GCACAATCTA CAAAGCCAAA AGGTCATTGT GTGGCTGTGC GAGTGACTAG CGAGGACTCC 
GATGATGGTT TTAAGCCTAC AGGAGGAAAA GTGCAGGAGC TCAGCTTTAA AAGCAAGCCA 
AATGTGTGGG CATACTTCTC TGTTAAGTCC GGAGGAGGAA TTCATGAATT CTCAGATTCT 
CAATTTGGAC ATGTTTTTGC TTTTGGAGAA TCTAGAGCTT TAGCAATTTC AAATATGGTA 
CTAGGGTTGA AGGAAATTCA AATTCGAGGA GAAATCCGTA CCAATGTTGA TTACACAATT 
GATCTTCTGA ATGCTTCAGA CTACAGAGAC AACAAAATTC ACACAGGTTG GCTAGACAGT 
AGAATTGCAA TGCATGTTAG AGCAGAGAGG CCTATGTGGT ATTTGTCTGT TGTTGGAGCG 
GCACTCTATA ACGCTTCTGC TAGCAGTGCA GCTTTGATTT CAGACTATGT TGGCTATCTT 
GAAAAGGGGC AAATTCCTCC TAAGCACATT TCTCTTGTCC GTTCTCAAGT TTCTTTGAAC 
ATTGAAGGAA GCAAATACAT GATTGACATG ATACGAGGAG GACCTGGATG TTACAAATTG 
AAATTGAATC AATCAGAGAT AGAAGCGGAG ATACATACTT TATGTGATGG AGGTTTACTG 
ATGCAGTTGG ACGGGAACAG TCATGTAATA TATGCAGAGG AAGAAGCAGC TGGAACTCGC 
CTTCTAATAG ATGGAAGAAC TTGTTTTCTT CAGAATGATC ACGATCCATC AAAGTTAATT 
GCAGAGACAC CCTGCAAGCT TCTGAGATAT TTGGTTGTAG ATGACAGTCA CATTGATGCA 
GACACACCAT ATGCTGAAAT TGAGGTCATG AAGATGTGCA TGCCTCTTCT TTCCCCTGCT 
TCTGGGATTA TTCATTTCAA AAAGGCTGAA GGTCAAGCCA TGCAGGCCGG TGAACTTATA 
GCAAAGCTTG ATCTAGGTGA CCCTTCTGCA GTAAGAAAGG CTGAACCCTT CAACGGGAGC 
TTCCCAATCC TGGGTCCTCC TACTGCAATT TCAGGTAAGG TTCACCAGAA ATGTGCAGCA 
AGCTTAAACG CTGCACGAAT GATTCTTGCT GGCTATGAGC AAAATATTGA TGAAGTTGTG 
CAAAGTTTGC TCAATTGCCT TGAAAACCCC GAATTGCCTT TCCTTCAATG GCAAAAGATT 
TTGGCTGTTA TGGCAACCCG ACTTCCTAAA GACCTAAGAA ATGAGTTGGA AGCAAAATAT 
AAGGAGTTCG AGAGTATTTC AAGCTCCCAA ATTATTGATT TTCCTGCGAA ATTATTAATG 
GGGATTCTTG AAGCCCATCT TTCCTCCTGT CTTGAAAAAG AAAAAGGAGC CTTAGAAAGA 
CTAATTGAAC CTCTGATGAG TCTTGTAAAA TCTTATGAGG GTGGAAGAGA GAGCCATGCT 
CATAAAATTG TTCAGTCTCT ATTTGAAGAG TATCTTTCAG TTGAAGAACT ATTCGGTGAT 
AATATTCAGG GTGATGTAAT TGAACGACTC CGTCTTCAAT CCAAGAAAGA TTTGTTCAAG 
ATTGTAGATG TTGTGCTCTC TCATCAGGGT GTCAAGAGAA AAAACAAACT GATACTGCGA 
CTAATGGATA AACTGGTTTA CCTTGATCCT GCTGCCTATA GTGACCAATT AATTCGATTC 
TCCAAACTCA ACCATATAGT TTATTCTGAG TTGGTTCTTA AGGCAAGTCA AATGTTAGAG 
CAAACTAAAC TGAGTGAACT TGGATCCAGC ATTGCTAGAA GTCTTTCTGA ACTAGAATTT 
TTTACCGAGG ATGGTGAAAA TACTGATACT CCGAAGAGGA AGAGTTACAT TAATGATCGA 
ATGGAAGACC TTGTGAGCTC TCCTTTGGCT GTTGAAGATG CTCTTGTTGG TTTATTTGAT 
CACAGTGATC ATGCCCTTAA ACGGAGGGCT GTGGAAACTT ATATCCGTAG GCTCTACCAG 
CAATATCTTG TCAAAGGGAG CTTAAGGATG CAGTGGCACA AATCTGGCCT TATTACTACA 
TGGGAATTCA TAGAAGAAAA TTTTGAACAG AAGAAATTGG GAGTGATGGT TGTAATTAAA 
TCTCTTCAAT TTTTGCCAGC AATAACCAGT GTTGCATTTA GGGAAGCAAC CAAGAACTTT 
CACGATGCAT TTAAAAGTGG TTCTGGCGAC TCAAGTAACC ATGGGAATGT GATGCATATT 
GGATTAGTGG GCATCAACAA CCAAACGAGT TTACTTCAAG ATAGTGGTGA TGAGGATCAG 
GATAAAAAAA GAATCGATAT GTTGATCAAA GTACTCAGAG AGCAGGAAGT AGGGACCATA 
ATACATGCTG CAGGTGTTGG AGATATTAGT TGTATCATAC ATAAGGATGA AGGGTTTGCT 
CCAATGAGGT ATTCCTTTCA CTGGTCAGCT GAAAAGCTAT ATTATGAAGA GGAACCTCTG 
TTGCGCCATC TGGAACCTCC CCTATCCATT TATCTTGAAT TGGACAAGCT TAAAGGCTAT 
GAAAATATCA GGTATACACC ATCCCAAGAT CATCAATGGC ACCTCTACAC TGTTGTGGAT 
ACTAAGCCAC TACCAATTCA AAGAATGTTT CTTCGAACAC TTCTCAAACA GCCAATCCGA 
AATGAAGGAC ACTCTTCTTA TCAAAGACTG GATGGAGAAA CGTCTCGTAC CCAATTGGCT 
ATGTCCTTTA CTTCAAGGAG CATTCTTAGG TCCCTAATGG GTGCAATGGA GGAGTTGGAA 
CTTAACTCAC ACAATACCAC CATCAAATCT GAACATGCTC ATATGTACCT CTATATCATC 
CGCAAGCAAC AAGTAGATGA TCTTGTGCCT AATTCCAAGA AAATTAACAT CGAAGTTGGT 
CAAGAAGAAA CAACTGTTGA GGCAATATTG GAAGATCTGG CACGGGAAAT CCATTCCTCT 
GTCGGTGTAA GAATGCACAG ATTAGGTGTT TTTGTGTGGG AAGTCAAGCT TTGGATTACA 
GCATATGGAC AAGCAAATGG TGCTTGGAGG GTCATTGTAA ACAACGTGAC TGGTCATACT 
TGCACCGTAC ATATATATCG AGAAATGGAG GATGCCACCG CTCGTAAAGT GGTTTACAGT 
TCAGTCAATG TAAAGGGTCC ACTGCATGGT GCATCAGTGA ACAAAAACTA TCAACCTTTG 
AGAGCTATTG ACCGAAAACG TCTTGCAGCT AGAAAGAACA GCACCACATA CTGCTATGAT 
TTTCCACTTG CATTTCAAAC ATCCTTGGAA CAGTCATGGT CAATTCAACA AACAAGAATT 
CAAATATCCA AAGGTAAGGA CCTCCTAAAA GTAACAGAGC TCAAATTTTC AGAGAAAGAA 
GGTATCTGCG GTACTCCTCT TGTACCTGTC GAACGTCCTC CTGGACTCAA TGATGTTGGC 
ATGGTAGCCT GGTTGATGGA TATGTATACG CCTGAATTCC CATCTGGAAG GACAATATTG 
GTTGTTTCGA ATGATGTGAC CTTCAAGGCC GGTTCTTTTG GCCCAGGAGA AGATGCGTAC 
TTTAGAGCAG TAACTGATCT TGCCTGTGCG AAGAAAATAC CTTTAATTTA CTTAGCAGCA 
AGTTCTGGTG CCCGGTTAGG TGTGGCAGAG GAAGTCAAAG CTTGTTTCAG AGTCGGTTGG 
TTTGAGGAAT CTAATCCTGA GCATGGTTTT CGCTATGTAT ATTTAACACC TGAGGATTAT 
GTTCGAATTA GATCATCCGT GATGGCACAT GAATTAAAGA TTGAAAGTGG AGAAACCAGA 
TGGGTTATAG ATACCATTCT CGGCAAAGAC GTTGGACTAG GAGTTGAAAA CTTGAGTGGC 
AGTGGGGCCA TTGCCGGTGC CTATTCAAGG GCATACAAGG AAACCTTTAC ACTAACATAT 
GTGACCGGTA CAACTGTTGG AATTGGTGCT TATCTTGCAA GGCTGGGGAT GAGATGCATA 
CAGAGGTTTG ATCAGCCTAT GATTCTTACC GGTTTTTCAG CATTAAACAA ACTCCTTGGT 
AGGGAAGTGT ACAGCTCTCA CATGCAACTT GGTGGATCTA AAATCATGGC AACAAATGGA 
GTTGTTCATC TTACAGTTTC GGATGACCTT GAAGGTGTTT CTTCTATTTT GAAGTGGCTT 
AGCTACATCC CTTCCCATGT AGGTGGTGCA CTTCCCGTTG TAAATCCCCT CGATTCCCCA 
GAGAGGGAAG TGGAATATTT ACCGGAAAAT TCATGTGATC CCCGTGCTGC CATTTCCGGA 
ATTCTAGATG TTAATGGAAA GTGGCTGGGA GGCATTTTTG ACAAGGACAG CTTTGTGGAG 
ACACTAGAAG GATGGGCTAG GACAGTTGTT ACAGGAAGGG CAAAGCTTGG TGGAATCCCT 
GTGGGAATCG TTGCGGTAGA AACACAGACA GTAATGCAAA TAATACCTGC TGATCCATGT 
CAGCTTGATT CTCATGAGAG GGTTGTTCCT CAAGCCGGCC AAGTGTGGTT TCCTGATTCT 
GCGACCAAAA CTGCCCAAGC GATATTGGAT TTCAATAGAG AAGAGCTCCC GCTTTTCATT 
CTCGCAAATT GGAGAGGCTT TTCGGGTGGG CAAAGGGACC TTTTCGAAGG AATTCTTCAG 
GCTGGTTCAA CTATTGTGGA GAACCTTAGA ACATACAAGC AACCCATTTT TGTATACATC 
CCAATGATGG GTGAACTCCG AGGTGGGGCA TGGGTTGTTG CTGACAGTCG AATAAATTCA 
GGCCATATTG AAACATATGC TGAGCGAACG GCTAAAGGTA ACGCCCTTGA ACCGGAAGGA 
ATGATTGAGA TCAAATTTAG AACAGGAGAA ATGTTGGAGT GTATGAGAAG ACTTGATCAG 
CAACTGATTA CTCTGAAGGA AAAACTTTCG GAAGCCAAGA GCAACAAGGA CTTTGGTACA 
TATGATTCTC TGCAACAGCA GATTAGATTC CGTGAGAAAC AGCTTTTGCC TTTGTATACT 
CAGATAGCTA TCAAGTTTGT TGAACTGCAT GATACTTCCT TAAAAATGGC TGCAATAGGC 
GCAATGAAAG AAGTTATTGA TTGGCGTGAC TCACGTTCTT TCTTCTATCG GAGACTGCAC 
AGGAGAATCG GTGAGCACTC ACTGATCAAC ATTGTGAGAG ATGCTGCTGG TGACCAATTG 
ACACATGTAT CTGCCATGAA CTTGCTCAAA GAATGGTATA TGAATTCTGA TATCTCCAAA 
GGTAGTGAAG ATGCTTGGTT GGACGATGAA GCCTTCTTCA GATGGAGGAA TGATCCCTCA 
AATTACGAGG ATAAACTAAA GGAATTGCGC ATCCAAAGAT TGTTGCTTCA GTTGAAAAAT 
ATTGGTGACT CGGATCTAGA TTTACAAGCT CTATCTCAAG GTCTTGCCAC CCTTTTAAGT 
AAGTTGGAAG CATCAGGTNN TGCAAAGTTG ACCGATGAAC TTCGCAAGGT ACTTGTGATC 
TGCCCATGCA CTCATGTTGT ATTGTGTTCA CGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013460844.2
CDS	342..7040
Translation	MASVGEYCNALGGNMPIHSILIANNGLAAVKFIRSVRSWAYETFGTENAIFLVAMATPEDMRINAEHIRIANQFVEVPGGTNNNNYANVQLILEIAEITHVDAVWPGWGHASEDLELPNALKEKGIVFLGPPAISMAALGDKIASLLIAQAAEVPTLPWSGSHVKFPPESDLITIPDEIYRASCVYTTEEAIASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLICDQYGNVAALHSRDCSVQRRHQKIIEEGPVTVAPPETVKELEHAARRLAKSVNYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVAVGMGIPLWKIPEIRRFYGMEHGGGNDAWRKTSVLGTPFDFDKAQSTKPKGHCVAVRVTSEDSDDGFKPTGGKVQELSFKSKPNVWAYFSVKSGGGIHEFSDSQFGHVFAFGESRALAISNMVLGLKEIQIRGEIRTNVDYTIDLLNASDYRDNKIHTGWLDSRIAMHVRAERPMWYLSVVGAALYNASASSAALISDYVGYLEKGQIPPKHISLVRSQVSLNIEGSKYMIDMIRGGPGCYKLKLNQSEIEAEIHTLCDGGLLMQLDGNSHVIYAEEEAAGTRLLIDGRTCFLQNDHDPSKLIAETPCKLLRYLVVDDSHIDADTPYAEIEVMKMCMPLLSPASGIIHFKKAEGQAMQAGELIAKLDLGDPSAVRKAEPFNGSFPILGPPTAISGKVHQKCAASLNAARMILAGYEQNIDEVVQSLLNCLENPELPFLQWQKILAVMATRLPKDLRNELEAKYKEFESISSSQIIDFPAKLLMGILEAHLSSCLEKEKGALERLIEPLMSLVKSYEGGRESHAHKIVQSLFEEYLSVEELFGDNIQGDVIERLRLQSKKDLFKIVDVVLSHQGVKRKNKLILRLMDKLVYLDPAAYSDQLIRFSKLNHIVYSELVLKASQMLEQTKLSELGSSIARSLSELEFFTEDGENTDTPKRKSYINDRMEDLVSSPLAVEDALVGLFDHSDHALKRRAVETYIRRLYQQYLVKGSLRMQWHKSGLITTWEFIEENFEQKKLGVMVVIKSLQFLPAITSVAFREATKNFHDAFKSGSGDSSNHGNVMHIGLVGINNQTSLLQDSGDEDQDKKRIDMLIKVLREQEVGTIIHAAGVGDISCIIHKDEGFAPMRYSFHWSAEKLYYEEEPLLRHLEPPLSIYLELDKLKGYENIRYTPSQDHQWHLYTVVDTKPLPIQRMFLRTLLKQPIRNEGHSSYQRLDGETSRTQLAMSFTSRSILRSLMGAMEELELNSHNTTIKSEHAHMYLYIIRKQQVDDLVPNSKKINIEVGQEETTVEAILEDLAREIHSSVGVRMHRLGVFVWEVKLWITAYGQANGAWRVIVNNVTGHTCTVHIYREMEDATARKVVYSSVNVKGPLHGASVNKNYQPLRAIDRKRLAARKNSTTYCYDFPLAFQTSLEQSWSIQQTRIQISKGKDLLKVTELKFSEKEGICGTPLVPVERPPGLNDVGMVAWLMDMYTPEFPSGRTILVVSNDVTFKAGSFGPGEDAYFRAVTDLACAKKIPLIYLAASSGARLGVAEEVKACFRVGWFEESNPEHGFRYVYLTPEDYVRIRSSVMAHELKIESGETRWVIDTILGKDVGLGVENLSGSGAIAGAYSRAYKETFTLTYVTGTTVGIGAYLARLGMRCIQRFDQPMILTGFSALNKLLGREVYSSHMQLGGSKIMATNGVVHLTVSDDLEGVSSILKWLSYIPSHVGGALPVVNPLDSPEREVEYLPENSCDPRAAISGILDVNGKWLGGIFDKDSFVETLEGWARTVVTGRAKLGGIPVGIVAVETQTVMQIIPADPCQLDSHERVVPQAGQVWFPDSATKTAQAILDFNREELPLFILANWRGFSGGQRDLFEGILQAGSTIVENLRTYKQPIFVYIPMMGELRGGAWVVADSRINSGHIETYAERTAKGNALEPEGMIEIKFRTGEMLECMRRLDQQLITLKEKLSEAKSNKDFGTYDSLQQQIRFREKQLLPLYTQIAIKFVELHDTSLKMAAIGAMKEVIDWRDSRSFFYRRLHRRIGEHSLINIVRDAAGDQLTHVSAMNLLKEWYMNSDISKGSEDAWLDDEAFFRWRNDPSNYEDKLKELRIQRLLLQLKNIGDSDLDLQALSQGLATLLSKLEASGnullAKLTDELRKVLVICPCTHVVLCSRH

Target ORF information:

RefSeq Version	XM_013605390.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula acetyl-CoA carboxylase 1 (LOC25489411), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013605390.2

ORF Insert Sequence:

ATGGCTAGCG TGGGTGAATA CTGCAATGCC CTTGGGGGAA ACATGCCAAT TCATAGTATT 
TTGATTGCAA ACAATGGATT GGCTGCAGTC AAGTTTATTC GCAGCGTTAG GAGTTGGGCT 
TATGAGACGT TCGGAACGGA AAATGCTATT TTCTTGGTTG CCATGGCTAC TCCAGAGGAC 
ATGAGAATCA ATGCAGAACA TATAAGAATT GCTAATCAGT TTGTGGAAGT ACCTGGTGGG 
ACCAATAACA ATAACTATGC CAATGTGCAA CTTATTCTAG AGATTGCTGA GATAACTCAC 
GTTGATGCGG TGTGGCCTGG TTGGGGTCAT GCATCAGAGG ATCTTGAGCT TCCAAATGCA 
TTGAAAGAAA AAGGAATTGT ATTTCTTGGA CCTCCTGCTA TATCTATGGC AGCTTTGGGA 
GATAAAATTG CTTCATTGTT GATTGCTCAA GCAGCAGAAG TGCCAACCCT TCCATGGAGT 
GGTTCTCATG TGAAATTTCC TCCAGAAAGC GACTTGATTA CTATTCCTGA TGAAATTTAC 
CGTGCATCAT GTGTTTATAC AACAGAAGAA GCAATTGCAA GTTGTCAAGT TGTAGGTTAC 
CCTGCAATGA TCAAGGCATC ATGGGGGGGC GGCGGTAAAG GCATAAGAAA GGTTCATAAT 
GATGACGAGG TAAGGGCATT GTTCAAGCAA GTTCAAGGTG AAGTTCCAGG CTCACCTATA 
TTTATAATGA AAGTTGCTTC CCAGAGCCGA CATCTTGAAG TCCAATTGAT TTGTGATCAG 
TATGGAAATG TTGCAGCTTT GCACAGCCGT GATTGCAGTG TTCAAAGAAG GCATCAAAAG 
ATTATTGAGG AAGGTCCCGT TACTGTAGCA CCTCCAGAAA CAGTGAAAGA ACTTGAACAT 
GCGGCTAGAA GATTAGCTAA ATCTGTAAAT TATGTTGGGG CAGCTACCGT TGAGTATCTT 
TATAGCATGG AAACTGGCGA GTACTACTTT TTAGAGTTGA ACCCACGACT ACAGGTTGAG 
CATCCTGTTA CTGAATGGAT AGCTGAGATA AATCTGCCAG CAGCACAAGT TGCAGTTGGG 
ATGGGTATCC CTCTCTGGAA AATTCCTGAG ATTAGGCGTT TCTATGGGAT GGAACATGGT 
GGGGGAAATG ATGCTTGGAG GAAAACATCA GTTTTAGGTA CCCCTTTTGA TTTTGACAAA 
GCACAATCTA CAAAGCCAAA AGGTCATTGT GTGGCTGTGC GAGTGACTAG CGAGGACTCC 
GATGATGGTT TTAAGCCTAC AGGAGGAAAA GTGCAGGAGC TCAGCTTTAA AAGCAAGCCA 
AATGTGTGGG CATACTTCTC TGTTAAGTCC GGAGGAGGAA TTCATGAATT CTCAGATTCT 
CAATTTGGAC ATGTTTTTGC TTTTGGAGAA TCTAGAGCTT TAGCAATTTC AAATATGGTA 
CTAGGGTTGA AGGAAATTCA AATTCGAGGA GAAATCCGTA CCAATGTTGA TTACACAATT 
GATCTTCTGA ATGCTTCAGA CTACAGAGAC AACAAAATTC ACACAGGTTG GCTAGACAGT 
AGAATTGCAA TGCATGTTAG AGCAGAGAGG CCTATGTGGT ATTTGTCTGT TGTTGGAGCG 
GCACTCTATA ACGCTTCTGC TAGCAGTGCA GCTTTGATTT CAGACTATGT TGGCTATCTT 
GAAAAGGGGC AAATTCCTCC TAAGCACATT TCTCTTGTCC GTTCTCAAGT TTCTTTGAAC 
ATTGAAGGAA GCAAATACAT GATTGACATG ATACGAGGAG GACCTGGATG TTACAAATTG 
AAATTGAATC AATCAGAGAT AGAAGCGGAG ATACATACTT TATGTGATGG AGGTTTACTG 
ATGCAGTTGG ACGGGAACAG TCATGTAATA TATGCAGAGG AAGAAGCAGC TGGAACTCGC 
CTTCTAATAG ATGGAAGAAC TTGTTTTCTT CAGAATGATC ACGATCCATC AAAGTTAATT 
GCAGAGACAC CCTGCAAGCT TCTGAGATAT TTGGTTGTAG ATGACAGTCA CATTGATGCA 
GACACACCAT ATGCTGAAAT TGAGGTCATG AAGATGTGCA TGCCTCTTCT TTCCCCTGCT 
TCTGGGATTA TTCATTTCAA AAAGGCTGAA GGTCAAGCCA TGCAGGCCGG TGAACTTATA 
GCAAAGCTTG ATCTAGGTGA CCCTTCTGCA GTAAGAAAGG CTGAACCCTT CAACGGGAGC 
TTCCCAATCC TGGGTCCTCC TACTGCAATT TCAGGTAAGG TTCACCAGAA ATGTGCAGCA 
AGCTTAAACG CTGCACGAAT GATTCTTGCT GGCTATGAGC AAAATATTGA TGAAGTTGTG 
CAAAGTTTGC TCAATTGCCT TGAAAACCCC GAATTGCCTT TCCTTCAATG GCAAAAGATT 
TTGGCTGTTA TGGCAACCCG ACTTCCTAAA GACCTAAGAA ATGAGTTGGA AGCAAAATAT 
AAGGAGTTCG AGAGTATTTC AAGCTCCCAA ATTATTGATT TTCCTGCGAA ATTATTAATG 
GGGATTCTTG AAGCCCATCT TTCCTCCTGT CTTGAAAAAG AAAAAGGAGC CTTAGAAAGA 
CTAATTGAAC CTCTGATGAG TCTTGTAAAA TCTTATGAGG GTGGAAGAGA GAGCCATGCT 
CATAAAATTG TTCAGTCTCT ATTTGAAGAG TATCTTTCAG TTGAAGAACT ATTCGGTGAT 
AATATTCAGG GTGATGTAAT TGAACGACTC CGTCTTCAAT CCAAGAAAGA TTTGTTCAAG 
ATTGTAGATG TTGTGCTCTC TCATCAGGGT GTCAAGAGAA AAAACAAACT GATACTGCGA 
CTAATGGATA AACTGGTTTA CCTTGATCCT GCTGCCTATA GTGACCAATT AATTCGATTC 
TCCAAACTCA ACCATATAGT TTATTCTGAG TTGGTTCTTA AGGCAAGTCA AATGTTAGAG 
CAAACTAAAC TGAGTGAACT TGGATCCAGC ATTGCTAGAA GTCTTTCTGA ACTAGAATTT 
TTTACCGAGG ATGGTGAAAA TACTGATACT CCGAAGAGGA AGAGTTACAT TAATGATCGA 
ATGGAAGACC TTGTGAGCTC TCCTTTGGCT GTTGAAGATG CTCTTGTTGG TTTATTTGAT 
CACAGTGATC ATGCCCTTAA ACGGAGGGCT GTGGAAACTT ATATCCGTAG GCTCTACCAG 
CAATATCTTG TCAAAGGGAG CTTAAGGATG CAGTGGCACA AATCTGGCCT TATTACTACA 
TGGGAATTCA TAGAAGAAAA TTTTGAACAG AAGAAATTGG GAGTGATGGT TGTAATTAAA 
TCTCTTCAAT TTTTGCCAGC AATAACCAGT GTTGCATTTA GGGAAGCAAC CAAGAACTTT 
CACGATGCAT TTAAAAGTGG TTCTGGCGAC TCAAGTAACC ATGGGAATGT GATGCATATT 
GGATTAGTGG GCATCAACAA CCAAACGAGT TTACTTCAAG ATAGTGGTGA TGAGGATCAG 
GATAAAAAAA GAATCGATAT GTTGATCAAA GTACTCAGAG AGCAGGAAGT AGGGACCATA 
ATACATGCTG CAGGTGTTGG AGATATTAGT TGTATCATAC ATAAGGATGA AGGGTTTGCT 
CCAATGAGGT ATTCCTTTCA CTGGTCAGCT GAAAAGCTAT ATTATGAAGA GGAACCTCTG 
TTGCGCCATC TGGAACCTCC CCTATCCATT TATCTTGAAT TGGACAAGCT TAAAGGCTAT 
GAAAATATCA GGTATACACC ATCCCAAGAT CATCAATGGC ACCTCTACAC TGTTGTGGAT 
ACTAAGCCAC TACCAATTCA AAGAATGTTT CTTCGAACAC TTCTCAAACA GCCAATCCGA 
AATGAAGGAC ACTCTTCTTA TCAAAGACTG GATGGAGAAA CGTCTCGTAC CCAATTGGCT 
ATGTCCTTTA CTTCAAGGAG CATTCTTAGG TCCCTAATGG GTGCAATGGA GGAGTTGGAA 
CTTAACTCAC ACAATACCAC CATCAAATCT GAACATGCTC ATATGTACCT CTATATCATC 
CGCAAGCAAC AAGTAGATGA TCTTGTGCCT AATTCCAAGA AAATTAACAT CGAAGTTGGT 
CAAGAAGAAA CAACTGTTGA GGCAATATTG GAAGATCTGG CACGGGAAAT CCATTCCTCT 
GTCGGTGTAA GAATGCACAG ATTAGGTGTT TTTGTGTGGG AAGTCAAGCT TTGGATTACA 
GCATATGGAC AAGCAAATGG TGCTTGGAGG GTCATTGTAA ACAACGTGAC TGGTCATACT 
TGCACCGTAC ATATATATCG AGAAATGGAG GATGCCACCG CTCGTAAAGT GGTTTACAGT 
TCAGTCAATG TAAAGGGTCC ACTGCATGGT GCATCAGTGA ACAAAAACTA TCAACCTTTG 
AGAGCTATTG ACCGAAAACG TCTTGCAGCT AGAAAGAACA GCACCACATA CTGCTATGAT 
TTTCCACTTG CATTTCAAAC ATCCTTGGAA CAGTCATGGT CAATTCAACA AACAAGAATT 
CAAATATCCA AAGGTAAGGA CCTCCTAAAA GTAACAGAGC TCAAATTTTC AGAGAAAGAA 
GGTATCTGCG GTACTCCTCT TGTACCTGTC GAACGTCCTC CTGGACTCAA TGATGTTGGC 
ATGGTAGCCT GGTTGATGGA TATGTATACG CCTGAATTCC CATCTGGAAG GACAATATTG 
GTTGTTTCGA ATGATGTGAC CTTCAAGGCC GGTTCTTTTG GCCCAGGAGA AGATGCGTAC 
TTTAGAGCAG TAACTGATCT TGCCTGTGCG AAGAAAATAC CTTTAATTTA CTTAGCAGCA 
AGTTCTGGTG CCCGGTTAGG TGTGGCAGAG GAAGTCAAAG CTTGTTTCAG AGTCGGTTGG 
TTTGAGGAAT CTAATCCTGA GCATGGTTTT CGCTATGTAT ATTTAACACC TGAGGATTAT 
GTTCGAATTA GATCATCCGT GATGGCACAT GAATTAAAGA TTGAAAGTGG AGAAACCAGA 
TGGGTTATAG ATACCATTCT CGGCAAAGAC GTTGGACTAG GAGTTGAAAA CTTGAGTGGC 
AGTGGGGCCA TTGCCGGTGC CTATTCAAGG GCATACAAGG AAACCTTTAC ACTAACATAT 
GTGACCGGTA CAACTGTTGG AATTGGTGCT TATCTTGCAA GGCTGGGGAT GAGATGCATA 
CAGAGGTTTG ATCAGCCTAT GATTCTTACC GGTTTTTCAG CATTAAACAA ACTCCTTGGT 
AGGGAAGTGT ACAGCTCTCA CATGCAACTT GGTGGATCTA AAATCATGGC AACAAATGGA 
GTTGTTCATC TTACAGTTTC GGATGACCTT GAAGGTGTTT CTTCTATTTT GAAGTGGCTT 
AGCTACATCC CTTCCCATGT AGGTGGTGCA CTTCCCGTTG TAAATCCCCT CGATTCCCCA 
GAGAGGGAAG TGGAATATTT ACCGGAAAAT TCATGTGATC CCCGTGCTGC CATTTCCGGA 
ATTCTAGATG TTAATGGAAA GTGGCTGGGA GGCATTTTTG ACAAGGACAG CTTTGTGGAG 
ACACTAGAAG GATGGGCTAG GACAGTTGTT ACAGGAAGGG CAAAGCTTGG TGGAATCCCT 
GTGGGAATCG TTGCGGTAGA AACACAGACA GTAATGCAAA TAATACCTGC TGATCCATGT 
CAGCTTGATT CTCATGAGAG GGTTGTTCCT CAAGCCGGCC AAGTGTGGTT TCCTGATTCT 
GCGACCAAAA CTGCCCAAGC GATATTGGAT TTCAATAGAG AAGAGCTCCC GCTTTTCATT 
CTCGCAAATT GGAGAGGCTT TTCGGGTGGG CAAAGGGACC TTTTCGAAGG AATTCTTCAG 
GCTGGTTCAA CTATTGTGGA GAACCTTAGA ACATACAAGC AACCCATTTT TGTATACATC 
CCAATGATGG GTGAACTCCG AGGTGGGGCA TGGGTTGTTG CTGACAGTCG AATAAATTCA 
GGCCATATTG AAACATATGC TGAGCGAACG GCTAAAGGTA ACGCCCTTGA ACCGGAAGGA 
ATGATTGAGA TCAAATTTAG AACAGGAGAA ATGTTGGAGT GTATGAGAAG ACTTGATCAG 
CAACTGATTA CTCTGAAGGA AAAACTTTCG GAAGCCAAGA GCAACAAGGA CTTTGGTACA 
TATGATTCTC TGCAACAGCA GATTAGATTC CGTGAGAAAC AGCTTTTGCC TTTGTATACT 
CAGATAGCTA TCAAGTTTGT TGAACTGCAT GATACTTCCT TAAAAATGGC TGCAATAGGC 
GCAATGAAAG AAGTTATTGA TTGGCGTGAC TCACGTTCTT TCTTCTATCG GAGACTGCAC 
AGGAGAATCG GTGAGCACTC ACTGATCAAC ATTGTGAGAG ATGCTGCTGG TGACCAATTG 
ACACATGTAT CTGCCATGAA CTTGCTCAAA GAATGGTATA TGAATTCTGA TATCTCCAAA 
GGTAGTGAAG ATGCTTGGTT GGACGATGAA GCCTTCTTCA GATGGAGGAA TGATCCCTCA 
AATTACGAGG ATAAACTAAA GGAATTGCGC ATCCAAAGAT TGTTGCTTCA GTTGAAAAAT 
ATTGGTGACT CGGATCTAGA TTTACAAGCT CTATCTCAAG GTCTTGCCAC CCTTTTAAGT 
AAGTTGGAAG CATCAGGTNN TGCAAAGTTG ACCGATGAAC TTCGCAAGGT ACTTGTGATC 
TGCCCATGCA CTCATGTTGT ATTGTGTTCA CGTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25489411 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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