LOC25484854 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25484854
Entrez Gene ID	25484854
Full Name	UDP-glycosyltransferase family protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013613861.1	XP_013469315.1	UDP-glycosyltransferase family protein
XM_024784580.1	XP_024640348.1	uncharacterized protein LOC25484854 isoform X2
XM_013613861.2	XP_013469315.1	uncharacterized protein LOC25484854 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25484854 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25484854 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe258152	XM_024784580.1 Latest version!	Medicago truncatula uncharacterized LOC25484854 (LOC25484854), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMe212662	XM_013613861.2 Latest version!	Medicago truncatula uncharacterized LOC25484854 (LOC25484854), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OMe212662	XM_013613861.1	Medicago truncatula UDP-glycosyltransferase family protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25484854 ( XM_024784580.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024640348.1 Medicago truncatula uncharacterized LOC25484854 (LOC25484854), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe258152
Clone ID Related Accession (Same CDS sequence)	XM_024784580.1
Accession Version	XM_024784580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	uncharacterized protein LOC25484854 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTCT CTCGCACCTC CTCCTCTCAA CCGGAGATCG ACGACACCGG CGGCACCGAC 
GTCGCATTCT CCTCGATCCG TGGTCGATTT CCTTTCAAAC GAAACCCTAG CCACCACCGA 
CAAAAATCTC TCTCTTCCGA TCGTCAATTA CCTCGCTCTT CCACTCGATC TCATCTCCAT 
AACCGTTTCT CTCGCAAAAG CTTACTCTCT CTCTTCCCTA AGTCCGGTTT CTACGCATTG 
ATCTTCGCTG TTGTGTTTCT CTTCGCGTTT GCTTCTATGG TTATGCAGAG TTCTATTACG 
TCCGTTTTTC GACAGAGGAA TGAGCGTGGA AGGAATCTTC GTGAAGGTCT TGAGTTTGGT 
AGTACGCTTA AGTTTGTTCC TGGGAAAGTT TCGCAGAGGT TTCTTTCTTG GGATGCACTT 
GATCGTTTGA GGTTTCAGCC TAGGATTGGC GTTCGTGCTC CCAGAATTGC TCTAATTTTA 
GGGCACATGA CGGTAGATCC CCAATCATTG ATGTTGGTTA CTGTAATTCA GAATCTACAG 
AAGTTGGGTT ATGTTTTTAA GATTTTTGGA GTAGGGCGTG GGAACGCTCG CTCCATATGG 
GAAAACATAG GTGGTGGCCT TTCTCCTTTC AGTACAGATC AGCAAGGCCA GATTGATTGG 
TCAAACTTTG AAGGTATCAT TGTTGACTCC CTAGAAGCAA AAGAAGCCAT TTCAAGCCTT 
ATGCAGGAGC CTTTTTGTTC AGTACCATTG ATATGGATTA TTCAAGAAGA TAGCCTTTCA 
AATCGTCTTC CAGTTTACAA ACAAATGGGT TGGCAGCATC TTATTTCTCA CTGGAGAAGT 
GCTTTTAGCA GAGCCAGTGT CATTGTCTTT CCAGATTTTA CTTATCCGAT GTTATATAGC 
GAGCTTGACA CTGGAAACTT TTTTGTGATC CCTGGATCGC CAGTAGATGT ATGGGCTGCA 
GAAAGCTATA GTAAAACCCA CACCAAGGAT CAGCTCAGAG AACTTAGTGG ATTTGGTAAA 
AATGATATGG TGGTTTTAGT TGTTGGGAGC TCAATCTTCT ATGATGACCT ATCTTGGGAA 
TATGCTGTTG CAATGAATTC CATAGGGCCT CTGCTGACAA AATATGCAAG GAGGAATGAT 
GCAGCCGAGT CATTCAAGTT TGTTTTCCTT TGTGGCAATT CAACTGATGG TTATGATGAC 
GCTTTACAGG AAGTTGCTTC ACGTTTGGGA CTTCCTCATG GTTCTATAAG ACATTATGGC 
TTGAATGGTG ATGTGAATAG TGTGTTACTG ATAGCTGATA TTGTCCTATA TGGTTCTGCT 
CAAGTTGTAC AGGGTTTTCC TCCATTATTA ATCAGAGCAA TGACTTTTGA AATCCCTGTC 
ATTGCACCTG ATTTTCCTGT GTTAAGAAAA TATATTCTTG ATGGGGTTCA TGGAGTTTTT 
TATTCTAAAC ACAATCCTGA AGCTTTGATG AATGCTTTTT CGCTTTTGCT TTCAAGTGGA 
AGACTTTCTA AATTTGCTCA AGCTATTGGA TCATCTGGAA GACAATTTGC TAAAAATGGG 
CTTGCTTTGG ATTGCATAAT TGGCTATGCA AGACTTCTGG AGAATGTACT AAGTTTTCCT 
TCAGATTCTT TACTACCTGG TCCTGTTTCT CAAATCCAAC AAGTAGCATG GGGATGGAGT 
TTTTTCCAGA ATGAAATAGA GCTAGATATT GACTTGCTAA AAATGGATGA TGATTTTTCT 
AATGGAAAAG CTACCGTTGT CCATGCCGTT GAGAAAGAGT TGGCTAGTCT TAATTATTCA 
ACAAACTTCC TTGAGAATGG AACAGATGTT CCAATACAAG ATGAACTGAC CAAATTGGAC 
TGGGACATTC TGAGAGAAAT TGAAATATCT GAAGAAAGTG AAATGCTCGA AATTGAACAG 
GTTGAAGAGA GGTTGGAGAA AGATGTTGGT GTTTGGGATG AGATATATCG TAATGCTAGG 
AAATCTGAGA AACTGAAGTT TGAAGCCAAT GAGCGGGATG AAGGGGAGCT TGAGAGAACA 
GGACAACCTG TGTGCATTTA TGAGATATAC AGTGGTGCTG GAGTGTGGCC ATTTTTACAC 
CATGGATCTT TATATCGTGG GTTAAGCCTT TCTAGAAGAT CCCAAAGACA AAGCTCTGAT 
GATGTGGATG CTGTTGGTCG TTTGCCTCTT CTGAATGACA CATATTACCG TGACATTCTC 
TGTGAAATGG GAGGAATGTT TGCAATTGCA AATAGAGTGG ATAGTATTCA CAGGAGGCCT 
TGGATTGGGT TTCAGTCATG GCGTGCTGCT GGTAGCATTG TCAGTGGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024640348.1
CDS	226..2559
Translation	MGLSRTSSSQPEIDDTGGTDVAFSSIRGRFPFKRNPSHHRQKSLSSDRQLPRSSTRSHLHNRFSRKSLLSLFPKSGFYALIFAVVFLFAFASMVMQSSITSVFRQRNERGRNLREGLEFGSTLKFVPGKVSQRFLSWDALDRLRFQPRIGVRAPRIALILGHMTVDPQSLMLVTVIQNLQKLGYVFKIFGVGRGNARSIWENIGGGLSPFSTDQQGQIDWSNFEGIIVDSLEAKEAISSLMQEPFCSVPLIWIIQEDSLSNRLPVYKQMGWQHLISHWRSAFSRASVIVFPDFTYPMLYSELDTGNFFVIPGSPVDVWAAESYSKTHTKDQLRELSGFGKNDMVVLVVGSSIFYDDLSWEYAVAMNSIGPLLTKYARRNDAAESFKFVFLCGNSTDGYDDALQEVASRLGLPHGSIRHYGLNGDVNSVLLIADIVLYGSAQVVQGFPPLLIRAMTFEIPVIAPDFPVLRKYILDGVHGVFYSKHNPEALMNAFSLLLSSGRLSKFAQAIGSSGRQFAKNGLALDCIIGYARLLENVLSFPSDSLLPGPVSQIQQVAWGWSFFQNEIELDIDLLKMDDDFSNGKATVVHAVEKELASLNYSTNFLENGTDVPIQDELTKLDWDILREIEISEESEMLEIEQVEERLEKDVGVWDEIYRNARKSEKLKFEANERDEGELERTGQPVCIYEIYSGAGVWPFLHHGSLYRGLSLSRRSQRQSSDDVDAVGRLPLLNDTYYRDILCEMGGMFAIANRVDSIHRRPWIGFQSWRAAGSIVSGS

Target ORF information:

RefSeq Version	XM_024784580.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula uncharacterized LOC25484854 (LOC25484854), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024784580.1

ORF Insert Sequence:

ATGGGACTCT CTCGCACCTC CTCCTCTCAA CCGGAGATCG ACGACACCGG CGGCACCGAC 
GTCGCATTCT CCTCGATCCG TGGTCGATTT CCTTTCAAAC GAAACCCTAG CCACCACCGA 
CAAAAATCTC TCTCTTCCGA TCGTCAATTA CCTCGCTCTT CCACTCGATC TCATCTCCAT 
AACCGTTTCT CTCGCAAAAG CTTACTCTCT CTCTTCCCTA AGTCCGGTTT CTACGCATTG 
ATCTTCGCTG TTGTGTTTCT CTTCGCGTTT GCTTCTATGG TTATGCAGAG TTCTATTACG 
TCCGTTTTTC GACAGAGGAA TGAGCGTGGA AGGAATCTTC GTGAAGGTCT TGAGTTTGGT 
AGTACGCTTA AGTTTGTTCC TGGGAAAGTT TCGCAGAGGT TTCTTTCTTG GGATGCACTT 
GATCGTTTGA GGTTTCAGCC TAGGATTGGC GTTCGTGCTC CCAGAATTGC TCTAATTTTA 
GGGCACATGA CGGTAGATCC CCAATCATTG ATGTTGGTTA CTGTAATTCA GAATCTACAG 
AAGTTGGGTT ATGTTTTTAA GATTTTTGGA GTAGGGCGTG GGAACGCTCG CTCCATATGG 
GAAAACATAG GTGGTGGCCT TTCTCCTTTC AGTACAGATC AGCAAGGCCA GATTGATTGG 
TCAAACTTTG AAGGTATCAT TGTTGACTCC CTAGAAGCAA AAGAAGCCAT TTCAAGCCTT 
ATGCAGGAGC CTTTTTGTTC AGTACCATTG ATATGGATTA TTCAAGAAGA TAGCCTTTCA 
AATCGTCTTC CAGTTTACAA ACAAATGGGT TGGCAGCATC TTATTTCTCA CTGGAGAAGT 
GCTTTTAGCA GAGCCAGTGT CATTGTCTTT CCAGATTTTA CTTATCCGAT GTTATATAGC 
GAGCTTGACA CTGGAAACTT TTTTGTGATC CCTGGATCGC CAGTAGATGT ATGGGCTGCA 
GAAAGCTATA GTAAAACCCA CACCAAGGAT CAGCTCAGAG AACTTAGTGG ATTTGGTAAA 
AATGATATGG TGGTTTTAGT TGTTGGGAGC TCAATCTTCT ATGATGACCT ATCTTGGGAA 
TATGCTGTTG CAATGAATTC CATAGGGCCT CTGCTGACAA AATATGCAAG GAGGAATGAT 
GCAGCCGAGT CATTCAAGTT TGTTTTCCTT TGTGGCAATT CAACTGATGG TTATGATGAC 
GCTTTACAGG AAGTTGCTTC ACGTTTGGGA CTTCCTCATG GTTCTATAAG ACATTATGGC 
TTGAATGGTG ATGTGAATAG TGTGTTACTG ATAGCTGATA TTGTCCTATA TGGTTCTGCT 
CAAGTTGTAC AGGGTTTTCC TCCATTATTA ATCAGAGCAA TGACTTTTGA AATCCCTGTC 
ATTGCACCTG ATTTTCCTGT GTTAAGAAAA TATATTCTTG ATGGGGTTCA TGGAGTTTTT 
TATTCTAAAC ACAATCCTGA AGCTTTGATG AATGCTTTTT CGCTTTTGCT TTCAAGTGGA 
AGACTTTCTA AATTTGCTCA AGCTATTGGA TCATCTGGAA GACAATTTGC TAAAAATGGG 
CTTGCTTTGG ATTGCATAAT TGGCTATGCA AGACTTCTGG AGAATGTACT AAGTTTTCCT 
TCAGATTCTT TACTACCTGG TCCTGTTTCT CAAATCCAAC AAGTAGCATG GGGATGGAGT 
TTTTTCCAGA ATGAAATAGA GCTAGATATT GACTTGCTAA AAATGGATGA TGATTTTTCT 
AATGGAAAAG CTACCGTTGT CCATGCCGTT GAGAAAGAGT TGGCTAGTCT TAATTATTCA 
ACAAACTTCC TTGAGAATGG AACAGATGTT CCAATACAAG ATGAACTGAC CAAATTGGAC 
TGGGACATTC TGAGAGAAAT TGAAATATCT GAAGAAAGTG AAATGCTCGA AATTGAACAG 
GTTGAAGAGA GGTTGGAGAA AGATGTTGGT GTTTGGGATG AGATATATCG TAATGCTAGG 
AAATCTGAGA AACTGAAGTT TGAAGCCAAT GAGCGGGATG AAGGGGAGCT TGAGAGAACA 
GGACAACCTG TGTGCATTTA TGAGATATAC AGTGGTGCTG GAGTGTGGCC ATTTTTACAC 
CATGGATCTT TATATCGTGG GTTAAGCCTT TCTAGAAGAT CCCAAAGACA AAGCTCTGAT 
GATGTGGATG CTGTTGGTCG TTTGCCTCTT CTGAATGACA CATATTACCG TGACATTCTC 
TGTGAAATGG GAGGAATGTT TGCAATTGCA AATAGAGTGG ATAGTATTCA CAGGAGGCCT 
TGGATTGGGT TTCAGTCATG GCGTGCTGCT GGTAGCATTG TCAGTGGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25484854 ( XM_013613861.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013469315.1 Medicago truncatula UDP-glycosyltransferase family protein mRNA.

Price & Availability↑

CloneID	OMe212662
Clone ID Related Accession (Same CDS sequence)	XM_013613861.1 , XM_013613861.2
Accession Version	XM_013613861.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	UDP-glycosyltransferase family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGGACTCT CTCGCACCTC CTCCTCTCAA CCGGAGATCG ACGACACCGG CGGCACCGAC 
GTCGCATTCT CCTCGATCCG TGGTCGATTT CCTTTCAAAC GAAACCCTAG CCACCACCGA 
CAAAAATCTC TCTCTTCCGA TCGTCAATTA CCTCGCTCTT CCACTCGATC TCATCTCCAT 
AACCGTTTCT CTCGCAAAAG CTTACTCTCT CTCTTCCCTA AGTCCGGTTT CTACGCATTG 
ATCTTCGCTG TTGTGTTTCT CTTCGCGTTT GCTTCTATGG TTATGCAGAG TTCTATTACG 
TCCGTTTTTC GACAGAGGAA TGAGCGTGGA AGGAATCTTC GTGAAGGTCT TGAGTTTGGT 
AGTACGCTTA AGTTTGTTCC TGGGAAAGTT TCGCAGAGGT TTCTTTCTTG GGATGCACTT 
GATCGTTTGA GGTTTCAGCC TAGGATTGGC GTTCGTGCTC CCAGAATTGC TCTAATTTTA 
GGGCACATGA CGGTAGATCC CCAATCATTG ATGTTGGTTA CTGTAATTCA GAATCTACAG 
AAGTTGGGTT ATGTTTTTAA GATTTTTGGA GTAGGGCGTG GGAACGCTCG CTCCATATGG 
GAAAACATAG GTGGTGGCCT TTCTCCTTTC AGTACAGATC AGCAAGGCCA GATTGATTGG 
TCAAACTTTG AAGGTATCAT TGTTGACTCC CTAGAAGCAA AAGAAGCCAT TTCAAGCCTT 
ATGCAGGAGC CTTTTTGTTC AGTACCATTG ATATGGATTA TTCAAGAAGA TAGCCTTTCA 
AATCGTCTTC CAGTTTACAA ACAAATGGGT TGGCAGCATC TTATTTCTCA CTGGAGAAGT 
GCTTTTAGCA GAGCCAGTGT CATTGTCTTT CCAGATTTTA CTTATCCGAT GTTATATAGC 
GAGCTTGACA CTGGAAACTT TTTTGTGATC CCTGGATCGC CAGTAGATGT ATGGGCTGCA 
GAAAGCTATA GTAAAACCCA CACCAAGGAT CAGCTCAGAG AACTTAGTGG ATTTGGTAAA 
AATGATATGG TGGTTTTAGT TGTTGGGAGC TCAATCTTCT ATGATGACCT ATCTTGGGAA 
TATGCTGTTG CAATGAATTC CATAGGGCCT CTGCTGACAA AATATGCAAG GAGGAATGAT 
GCAGCCGAGT CATTCAAGTT TGTTTTCCTT TGTGGCAATT CAACTGATGG TTATGATGAC 
GCTTTACAGG AAGTTGCTTC ACGTTTGGGA CTTCCTCATG GTTCTATAAG ACATTATGGC 
TTGAATGGTG ATGTGAATAG TGTGTTACTG ATAGCTGATA TTGTCCTATA TGGTTCTGCT 
CAAGTTGTAC AGGGTTTTCC TCCATTATTA ATCAGAGCAA TGACTTTTGA AATCCCTGTC 
ATTGCACCTG ATTTTCCTGT GTTAAGAAAA TATATTCTTG ATGGGGTTCA TGGAGTTTTT 
TATTCTAAAC ACAATCCTGA AGCTTTGATG AATGCTTTTT CGCTTTTGCT TTCAAGTGGA 
AGACTTTCTA AATTTGCTCA AGCTATTGGA TCATCTGGAA GACAATTTGC TAAAAATGGG 
CTTGCTTTGG ATTGCATAAT TGGCTATGCA AGACTTCTGG AGAATGTACT AAGTTTTCCT 
TCAGATTCTT TACTACCTGG TCCTGTTTCT CAAATCCAAC AAGTAGCATG GGGATGGAGT 
TTTTTCCAGA ATGAAATAGA GCTAGATATT GACTTGCTAA AAATGGATGA TGATTTTTCT 
AATGGAAAAG CTACCGTTGT CCATGCCGTT GAGAAAGAGT TGGCTAGTCT TAATTATTCA 
ACAAACTTCC TTGAGAATGG AACAGATGTT CCAATACAAG ATGAACTGAC CAAATTGGAC 
TGGGACATTC TGAGAGAAAT TGAAATATCT GAAGAAAGTG AAATGCTCGA AATTGAACAG 
GTTGAAGAGA GGTTGGAGAA AGATGTTGGT GTTTGGGATG AGATATATCG TAATGCTAGG 
AAATCTGAGA AACTGAAGTT TGAAGCCAAT GAGCGGGATG AAGGGGAGCT TGAGAGAACA 
GGACAACCTG TGTGCATTTA TGAGATATAC AGTGGTGCTG GAGTGTGGCC ATTTTTACAC 
CATGGATCTT TATATCGTGG GTTAAGCCTT TCTAGAAGAT CCCAAAGACA AAGCTCTGAT 
GATGTGGATG CTGTTGGTCG TTTGCCTCTT CTGAATGACA CATATTACCG TGACATTCTC 
TGTGAAATGG GAGGAATGTT TGCAATTGCA AATAGAGTGG ATAGTATTCA CAGGAGGCCT 
TGGATTGGGT TTCAGTCATG GCGTGCTGCT GGTAGGAAGG TAGCATTGTC AGTGGAAGCT 
GAAAGTGTTT TGGAAGAGAC AATGCATGAG AATGCTAGAG GAGATGTAAT ATATTTTTGG 
GGACGCTTGG ACCTGGACGG GGGTGCCATA GGAAGCAACA ATGCACTTAC TTTTTGGTCA 
ATGTGTGATA TCTTAAATGG AGGGAACTGC AGGAATGTTT TTCAAGATAG CTTCCGCCAG 
ATGTATTCAT TGCCCCCCCA TGCTGAAGCG CTGCCCCCTA TGCCTGAAGA TGGTGGTTAT 
TGGTCAGCAC TGCACAGTTG GGTGATGCCA ACCCCTTCTT TTCTGGAGTT CGTAATGTTC 
TCTCGGATGT TCGTTGATTC CATCGATGCT TTTCACAGAG ACTCTGGTAA ATACAGCATG 
TGCTTGTTAG GCTCTTCAGA GATTGAGGAA AAACACTGTT ACTGTCGAAT GTTGGAACTT 
CTTATCAATG TATGGGCTTA TCATAGTTCA CGAAAGATGG TATATATAAA TCCTAATACT 
GGTTCTTTGC AAGAGCAGCA CCTAGTGGAA CAACGGAAGA GTTTCATGTG GGCAAAATAT 
TTCAATTTTT CATTGTTAAA AAGTATGGAT GAAGATCTAG CAGAAGCTGC AGATGATGGT 
GACGATCCAA GGGATAAGTG GTTGTGGCCA ATGACAGGAG AAGTGCACTG GCAAGGGATT 
TATGAAAGGG AGAGGGAAGA AAGGTACAGA ATAAAAATGG ATAAAAAAAG AAAAACAAAA 
GAGAAATTAT ACGAAAGGAT GAAGTATGGT TACAAGCAGA AATCACTTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013469315.1
CDS	227..3343
Translation	MGLSRTSSSQPEIDDTGGTDVAFSSIRGRFPFKRNPSHHRQKSLSSDRQLPRSSTRSHLHNRFSRKSLLSLFPKSGFYALIFAVVFLFAFASMVMQSSITSVFRQRNERGRNLREGLEFGSTLKFVPGKVSQRFLSWDALDRLRFQPRIGVRAPRIALILGHMTVDPQSLMLVTVIQNLQKLGYVFKIFGVGRGNARSIWENIGGGLSPFSTDQQGQIDWSNFEGIIVDSLEAKEAISSLMQEPFCSVPLIWIIQEDSLSNRLPVYKQMGWQHLISHWRSAFSRASVIVFPDFTYPMLYSELDTGNFFVIPGSPVDVWAAESYSKTHTKDQLRELSGFGKNDMVVLVVGSSIFYDDLSWEYAVAMNSIGPLLTKYARRNDAAESFKFVFLCGNSTDGYDDALQEVASRLGLPHGSIRHYGLNGDVNSVLLIADIVLYGSAQVVQGFPPLLIRAMTFEIPVIAPDFPVLRKYILDGVHGVFYSKHNPEALMNAFSLLLSSGRLSKFAQAIGSSGRQFAKNGLALDCIIGYARLLENVLSFPSDSLLPGPVSQIQQVAWGWSFFQNEIELDIDLLKMDDDFSNGKATVVHAVEKELASLNYSTNFLENGTDVPIQDELTKLDWDILREIEISEESEMLEIEQVEERLEKDVGVWDEIYRNARKSEKLKFEANERDEGELERTGQPVCIYEIYSGAGVWPFLHHGSLYRGLSLSRRSQRQSSDDVDAVGRLPLLNDTYYRDILCEMGGMFAIANRVDSIHRRPWIGFQSWRAAGRKVALSVEAESVLEETMHENARGDVIYFWGRLDLDGGAIGSNNALTFWSMCDILNGGNCRNVFQDSFRQMYSLPPHAEALPPMPEDGGYWSALHSWVMPTPSFLEFVMFSRMFVDSIDAFHRDSGKYSMCLLGSSEIEEKHCYCRMLELLINVWAYHSSRKMVYINPNTGSLQEQHLVEQRKSFMWAKYFNFSLLKSMDEDLAEAADDGDDPRDKWLWPMTGEVHWQGIYEREREERYRIKMDKKRKTKEKLYERMKYGYKQKSLGL

Target ORF information:

RefSeq Version	XM_013613861.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula UDP-glycosyltransferase family protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613861.1

ORF Insert Sequence:

ATGGGACTCT CTCGCACCTC CTCCTCTCAA CCGGAGATCG ACGACACCGG CGGCACCGAC 
GTCGCATTCT CCTCGATCCG TGGTCGATTT CCTTTCAAAC GAAACCCTAG CCACCACCGA 
CAAAAATCTC TCTCTTCCGA TCGTCAATTA CCTCGCTCTT CCACTCGATC TCATCTCCAT 
AACCGTTTCT CTCGCAAAAG CTTACTCTCT CTCTTCCCTA AGTCCGGTTT CTACGCATTG 
ATCTTCGCTG TTGTGTTTCT CTTCGCGTTT GCTTCTATGG TTATGCAGAG TTCTATTACG 
TCCGTTTTTC GACAGAGGAA TGAGCGTGGA AGGAATCTTC GTGAAGGTCT TGAGTTTGGT 
AGTACGCTTA AGTTTGTTCC TGGGAAAGTT TCGCAGAGGT TTCTTTCTTG GGATGCACTT 
GATCGTTTGA GGTTTCAGCC TAGGATTGGC GTTCGTGCTC CCAGAATTGC TCTAATTTTA 
GGGCACATGA CGGTAGATCC CCAATCATTG ATGTTGGTTA CTGTAATTCA GAATCTACAG 
AAGTTGGGTT ATGTTTTTAA GATTTTTGGA GTAGGGCGTG GGAACGCTCG CTCCATATGG 
GAAAACATAG GTGGTGGCCT TTCTCCTTTC AGTACAGATC AGCAAGGCCA GATTGATTGG 
TCAAACTTTG AAGGTATCAT TGTTGACTCC CTAGAAGCAA AAGAAGCCAT TTCAAGCCTT 
ATGCAGGAGC CTTTTTGTTC AGTACCATTG ATATGGATTA TTCAAGAAGA TAGCCTTTCA 
AATCGTCTTC CAGTTTACAA ACAAATGGGT TGGCAGCATC TTATTTCTCA CTGGAGAAGT 
GCTTTTAGCA GAGCCAGTGT CATTGTCTTT CCAGATTTTA CTTATCCGAT GTTATATAGC 
GAGCTTGACA CTGGAAACTT TTTTGTGATC CCTGGATCGC CAGTAGATGT ATGGGCTGCA 
GAAAGCTATA GTAAAACCCA CACCAAGGAT CAGCTCAGAG AACTTAGTGG ATTTGGTAAA 
AATGATATGG TGGTTTTAGT TGTTGGGAGC TCAATCTTCT ATGATGACCT ATCTTGGGAA 
TATGCTGTTG CAATGAATTC CATAGGGCCT CTGCTGACAA AATATGCAAG GAGGAATGAT 
GCAGCCGAGT CATTCAAGTT TGTTTTCCTT TGTGGCAATT CAACTGATGG TTATGATGAC 
GCTTTACAGG AAGTTGCTTC ACGTTTGGGA CTTCCTCATG GTTCTATAAG ACATTATGGC 
TTGAATGGTG ATGTGAATAG TGTGTTACTG ATAGCTGATA TTGTCCTATA TGGTTCTGCT 
CAAGTTGTAC AGGGTTTTCC TCCATTATTA ATCAGAGCAA TGACTTTTGA AATCCCTGTC 
ATTGCACCTG ATTTTCCTGT GTTAAGAAAA TATATTCTTG ATGGGGTTCA TGGAGTTTTT 
TATTCTAAAC ACAATCCTGA AGCTTTGATG AATGCTTTTT CGCTTTTGCT TTCAAGTGGA 
AGACTTTCTA AATTTGCTCA AGCTATTGGA TCATCTGGAA GACAATTTGC TAAAAATGGG 
CTTGCTTTGG ATTGCATAAT TGGCTATGCA AGACTTCTGG AGAATGTACT AAGTTTTCCT 
TCAGATTCTT TACTACCTGG TCCTGTTTCT CAAATCCAAC AAGTAGCATG GGGATGGAGT 
TTTTTCCAGA ATGAAATAGA GCTAGATATT GACTTGCTAA AAATGGATGA TGATTTTTCT 
AATGGAAAAG CTACCGTTGT CCATGCCGTT GAGAAAGAGT TGGCTAGTCT TAATTATTCA 
ACAAACTTCC TTGAGAATGG AACAGATGTT CCAATACAAG ATGAACTGAC CAAATTGGAC 
TGGGACATTC TGAGAGAAAT TGAAATATCT GAAGAAAGTG AAATGCTCGA AATTGAACAG 
GTTGAAGAGA GGTTGGAGAA AGATGTTGGT GTTTGGGATG AGATATATCG TAATGCTAGG 
AAATCTGAGA AACTGAAGTT TGAAGCCAAT GAGCGGGATG AAGGGGAGCT TGAGAGAACA 
GGACAACCTG TGTGCATTTA TGAGATATAC AGTGGTGCTG GAGTGTGGCC ATTTTTACAC 
CATGGATCTT TATATCGTGG GTTAAGCCTT TCTAGAAGAT CCCAAAGACA AAGCTCTGAT 
GATGTGGATG CTGTTGGTCG TTTGCCTCTT CTGAATGACA CATATTACCG TGACATTCTC 
TGTGAAATGG GAGGAATGTT TGCAATTGCA AATAGAGTGG ATAGTATTCA CAGGAGGCCT 
TGGATTGGGT TTCAGTCATG GCGTGCTGCT GGTAGGAAGG TAGCATTGTC AGTGGAAGCT 
GAAAGTGTTT TGGAAGAGAC AATGCATGAG AATGCTAGAG GAGATGTAAT ATATTTTTGG 
GGACGCTTGG ACCTGGACGG GGGTGCCATA GGAAGCAACA ATGCACTTAC TTTTTGGTCA 
ATGTGTGATA TCTTAAATGG AGGGAACTGC AGGAATGTTT TTCAAGATAG CTTCCGCCAG 
ATGTATTCAT TGCCCCCCCA TGCTGAAGCG CTGCCCCCTA TGCCTGAAGA TGGTGGTTAT 
TGGTCAGCAC TGCACAGTTG GGTGATGCCA ACCCCTTCTT TTCTGGAGTT CGTAATGTTC 
TCTCGGATGT TCGTTGATTC CATCGATGCT TTTCACAGAG ACTCTGGTAA ATACAGCATG 
TGCTTGTTAG GCTCTTCAGA GATTGAGGAA AAACACTGTT ACTGTCGAAT GTTGGAACTT 
CTTATCAATG TATGGGCTTA TCATAGTTCA CGAAAGATGG TATATATAAA TCCTAATACT 
GGTTCTTTGC AAGAGCAGCA CCTAGTGGAA CAACGGAAGA GTTTCATGTG GGCAAAATAT 
TTCAATTTTT CATTGTTAAA AAGTATGGAT GAAGATCTAG CAGAAGCTGC AGATGATGGT 
GACGATCCAA GGGATAAGTG GTTGTGGCCA ATGACAGGAG AAGTGCACTG GCAAGGGATT 
TATGAAAGGG AGAGGGAAGA AAGGTACAGA ATAAAAATGG ATAAAAAAAG AAAAACAAAA 
GAGAAATTAT ACGAAAGGAT GAAGTATGGT TACAAGCAGA AATCACTTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25484854 ( XM_013613861.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013469315.1 Medicago truncatula uncharacterized LOC25484854 (LOC25484854), transcript variant X1, mRNA.

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CloneID	OMe212662
Clone ID Related Accession (Same CDS sequence)	XM_013613861.1 , XM_013613861.2
Accession Version	XM_013613861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	uncharacterized protein LOC25484854 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013613861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTCT CTCGCACCTC CTCCTCTCAA CCGGAGATCG ACGACACCGG CGGCACCGAC 
GTCGCATTCT CCTCGATCCG TGGTCGATTT CCTTTCAAAC GAAACCCTAG CCACCACCGA 
CAAAAATCTC TCTCTTCCGA TCGTCAATTA CCTCGCTCTT CCACTCGATC TCATCTCCAT 
AACCGTTTCT CTCGCAAAAG CTTACTCTCT CTCTTCCCTA AGTCCGGTTT CTACGCATTG 
ATCTTCGCTG TTGTGTTTCT CTTCGCGTTT GCTTCTATGG TTATGCAGAG TTCTATTACG 
TCCGTTTTTC GACAGAGGAA TGAGCGTGGA AGGAATCTTC GTGAAGGTCT TGAGTTTGGT 
AGTACGCTTA AGTTTGTTCC TGGGAAAGTT TCGCAGAGGT TTCTTTCTTG GGATGCACTT 
GATCGTTTGA GGTTTCAGCC TAGGATTGGC GTTCGTGCTC CCAGAATTGC TCTAATTTTA 
GGGCACATGA CGGTAGATCC CCAATCATTG ATGTTGGTTA CTGTAATTCA GAATCTACAG 
AAGTTGGGTT ATGTTTTTAA GATTTTTGGA GTAGGGCGTG GGAACGCTCG CTCCATATGG 
GAAAACATAG GTGGTGGCCT TTCTCCTTTC AGTACAGATC AGCAAGGCCA GATTGATTGG 
TCAAACTTTG AAGGTATCAT TGTTGACTCC CTAGAAGCAA AAGAAGCCAT TTCAAGCCTT 
ATGCAGGAGC CTTTTTGTTC AGTACCATTG ATATGGATTA TTCAAGAAGA TAGCCTTTCA 
AATCGTCTTC CAGTTTACAA ACAAATGGGT TGGCAGCATC TTATTTCTCA CTGGAGAAGT 
GCTTTTAGCA GAGCCAGTGT CATTGTCTTT CCAGATTTTA CTTATCCGAT GTTATATAGC 
GAGCTTGACA CTGGAAACTT TTTTGTGATC CCTGGATCGC CAGTAGATGT ATGGGCTGCA 
GAAAGCTATA GTAAAACCCA CACCAAGGAT CAGCTCAGAG AACTTAGTGG ATTTGGTAAA 
AATGATATGG TGGTTTTAGT TGTTGGGAGC TCAATCTTCT ATGATGACCT ATCTTGGGAA 
TATGCTGTTG CAATGAATTC CATAGGGCCT CTGCTGACAA AATATGCAAG GAGGAATGAT 
GCAGCCGAGT CATTCAAGTT TGTTTTCCTT TGTGGCAATT CAACTGATGG TTATGATGAC 
GCTTTACAGG AAGTTGCTTC ACGTTTGGGA CTTCCTCATG GTTCTATAAG ACATTATGGC 
TTGAATGGTG ATGTGAATAG TGTGTTACTG ATAGCTGATA TTGTCCTATA TGGTTCTGCT 
CAAGTTGTAC AGGGTTTTCC TCCATTATTA ATCAGAGCAA TGACTTTTGA AATCCCTGTC 
ATTGCACCTG ATTTTCCTGT GTTAAGAAAA TATATTCTTG ATGGGGTTCA TGGAGTTTTT 
TATTCTAAAC ACAATCCTGA AGCTTTGATG AATGCTTTTT CGCTTTTGCT TTCAAGTGGA 
AGACTTTCTA AATTTGCTCA AGCTATTGGA TCATCTGGAA GACAATTTGC TAAAAATGGG 
CTTGCTTTGG ATTGCATAAT TGGCTATGCA AGACTTCTGG AGAATGTACT AAGTTTTCCT 
TCAGATTCTT TACTACCTGG TCCTGTTTCT CAAATCCAAC AAGTAGCATG GGGATGGAGT 
TTTTTCCAGA ATGAAATAGA GCTAGATATT GACTTGCTAA AAATGGATGA TGATTTTTCT 
AATGGAAAAG CTACCGTTGT CCATGCCGTT GAGAAAGAGT TGGCTAGTCT TAATTATTCA 
ACAAACTTCC TTGAGAATGG AACAGATGTT CCAATACAAG ATGAACTGAC CAAATTGGAC 
TGGGACATTC TGAGAGAAAT TGAAATATCT GAAGAAAGTG AAATGCTCGA AATTGAACAG 
GTTGAAGAGA GGTTGGAGAA AGATGTTGGT GTTTGGGATG AGATATATCG TAATGCTAGG 
AAATCTGAGA AACTGAAGTT TGAAGCCAAT GAGCGGGATG AAGGGGAGCT TGAGAGAACA 
GGACAACCTG TGTGCATTTA TGAGATATAC AGTGGTGCTG GAGTGTGGCC ATTTTTACAC 
CATGGATCTT TATATCGTGG GTTAAGCCTT TCTAGAAGAT CCCAAAGACA AAGCTCTGAT 
GATGTGGATG CTGTTGGTCG TTTGCCTCTT CTGAATGACA CATATTACCG TGACATTCTC 
TGTGAAATGG GAGGAATGTT TGCAATTGCA AATAGAGTGG ATAGTATTCA CAGGAGGCCT 
TGGATTGGGT TTCAGTCATG GCGTGCTGCT GGTAGGAAGG TAGCATTGTC AGTGGAAGCT 
GAAAGTGTTT TGGAAGAGAC AATGCATGAG AATGCTAGAG GAGATGTAAT ATATTTTTGG 
GGACGCTTGG ACCTGGACGG GGGTGCCATA GGAAGCAACA ATGCACTTAC TTTTTGGTCA 
ATGTGTGATA TCTTAAATGG AGGGAACTGC AGGAATGTTT TTCAAGATAG CTTCCGCCAG 
ATGTATTCAT TGCCCCCCCA TGCTGAAGCG CTGCCCCCTA TGCCTGAAGA TGGTGGTTAT 
TGGTCAGCAC TGCACAGTTG GGTGATGCCA ACCCCTTCTT TTCTGGAGTT CGTAATGTTC 
TCTCGGATGT TCGTTGATTC CATCGATGCT TTTCACAGAG ACTCTGGTAA ATACAGCATG 
TGCTTGTTAG GCTCTTCAGA GATTGAGGAA AAACACTGTT ACTGTCGAAT GTTGGAACTT 
CTTATCAATG TATGGGCTTA TCATAGTTCA CGAAAGATGG TATATATAAA TCCTAATACT 
GGTTCTTTGC AAGAGCAGCA CCTAGTGGAA CAACGGAAGA GTTTCATGTG GGCAAAATAT 
TTCAATTTTT CATTGTTAAA AAGTATGGAT GAAGATCTAG CAGAAGCTGC AGATGATGGT 
GACGATCCAA GGGATAAGTG GTTGTGGCCA ATGACAGGAG AAGTGCACTG GCAAGGGATT 
TATGAAAGGG AGAGGGAAGA AAGGTACAGA ATAAAAATGG ATAAAAAAAG AAAAACAAAA 
GAGAAATTAT ACGAAAGGAT GAAGTATGGT TACAAGCAGA AATCACTTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013469315.1
CDS	223..3339
Translation	MGLSRTSSSQPEIDDTGGTDVAFSSIRGRFPFKRNPSHHRQKSLSSDRQLPRSSTRSHLHNRFSRKSLLSLFPKSGFYALIFAVVFLFAFASMVMQSSITSVFRQRNERGRNLREGLEFGSTLKFVPGKVSQRFLSWDALDRLRFQPRIGVRAPRIALILGHMTVDPQSLMLVTVIQNLQKLGYVFKIFGVGRGNARSIWENIGGGLSPFSTDQQGQIDWSNFEGIIVDSLEAKEAISSLMQEPFCSVPLIWIIQEDSLSNRLPVYKQMGWQHLISHWRSAFSRASVIVFPDFTYPMLYSELDTGNFFVIPGSPVDVWAAESYSKTHTKDQLRELSGFGKNDMVVLVVGSSIFYDDLSWEYAVAMNSIGPLLTKYARRNDAAESFKFVFLCGNSTDGYDDALQEVASRLGLPHGSIRHYGLNGDVNSVLLIADIVLYGSAQVVQGFPPLLIRAMTFEIPVIAPDFPVLRKYILDGVHGVFYSKHNPEALMNAFSLLLSSGRLSKFAQAIGSSGRQFAKNGLALDCIIGYARLLENVLSFPSDSLLPGPVSQIQQVAWGWSFFQNEIELDIDLLKMDDDFSNGKATVVHAVEKELASLNYSTNFLENGTDVPIQDELTKLDWDILREIEISEESEMLEIEQVEERLEKDVGVWDEIYRNARKSEKLKFEANERDEGELERTGQPVCIYEIYSGAGVWPFLHHGSLYRGLSLSRRSQRQSSDDVDAVGRLPLLNDTYYRDILCEMGGMFAIANRVDSIHRRPWIGFQSWRAAGRKVALSVEAESVLEETMHENARGDVIYFWGRLDLDGGAIGSNNALTFWSMCDILNGGNCRNVFQDSFRQMYSLPPHAEALPPMPEDGGYWSALHSWVMPTPSFLEFVMFSRMFVDSIDAFHRDSGKYSMCLLGSSEIEEKHCYCRMLELLINVWAYHSSRKMVYINPNTGSLQEQHLVEQRKSFMWAKYFNFSLLKSMDEDLAEAADDGDDPRDKWLWPMTGEVHWQGIYEREREERYRIKMDKKRKTKEKLYERMKYGYKQKSLGL

Target ORF information:

RefSeq Version	XM_013613861.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula uncharacterized LOC25484854 (LOC25484854), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613861.2

ORF Insert Sequence:

ATGGGACTCT CTCGCACCTC CTCCTCTCAA CCGGAGATCG ACGACACCGG CGGCACCGAC 
GTCGCATTCT CCTCGATCCG TGGTCGATTT CCTTTCAAAC GAAACCCTAG CCACCACCGA 
CAAAAATCTC TCTCTTCCGA TCGTCAATTA CCTCGCTCTT CCACTCGATC TCATCTCCAT 
AACCGTTTCT CTCGCAAAAG CTTACTCTCT CTCTTCCCTA AGTCCGGTTT CTACGCATTG 
ATCTTCGCTG TTGTGTTTCT CTTCGCGTTT GCTTCTATGG TTATGCAGAG TTCTATTACG 
TCCGTTTTTC GACAGAGGAA TGAGCGTGGA AGGAATCTTC GTGAAGGTCT TGAGTTTGGT 
AGTACGCTTA AGTTTGTTCC TGGGAAAGTT TCGCAGAGGT TTCTTTCTTG GGATGCACTT 
GATCGTTTGA GGTTTCAGCC TAGGATTGGC GTTCGTGCTC CCAGAATTGC TCTAATTTTA 
GGGCACATGA CGGTAGATCC CCAATCATTG ATGTTGGTTA CTGTAATTCA GAATCTACAG 
AAGTTGGGTT ATGTTTTTAA GATTTTTGGA GTAGGGCGTG GGAACGCTCG CTCCATATGG 
GAAAACATAG GTGGTGGCCT TTCTCCTTTC AGTACAGATC AGCAAGGCCA GATTGATTGG 
TCAAACTTTG AAGGTATCAT TGTTGACTCC CTAGAAGCAA AAGAAGCCAT TTCAAGCCTT 
ATGCAGGAGC CTTTTTGTTC AGTACCATTG ATATGGATTA TTCAAGAAGA TAGCCTTTCA 
AATCGTCTTC CAGTTTACAA ACAAATGGGT TGGCAGCATC TTATTTCTCA CTGGAGAAGT 
GCTTTTAGCA GAGCCAGTGT CATTGTCTTT CCAGATTTTA CTTATCCGAT GTTATATAGC 
GAGCTTGACA CTGGAAACTT TTTTGTGATC CCTGGATCGC CAGTAGATGT ATGGGCTGCA 
GAAAGCTATA GTAAAACCCA CACCAAGGAT CAGCTCAGAG AACTTAGTGG ATTTGGTAAA 
AATGATATGG TGGTTTTAGT TGTTGGGAGC TCAATCTTCT ATGATGACCT ATCTTGGGAA 
TATGCTGTTG CAATGAATTC CATAGGGCCT CTGCTGACAA AATATGCAAG GAGGAATGAT 
GCAGCCGAGT CATTCAAGTT TGTTTTCCTT TGTGGCAATT CAACTGATGG TTATGATGAC 
GCTTTACAGG AAGTTGCTTC ACGTTTGGGA CTTCCTCATG GTTCTATAAG ACATTATGGC 
TTGAATGGTG ATGTGAATAG TGTGTTACTG ATAGCTGATA TTGTCCTATA TGGTTCTGCT 
CAAGTTGTAC AGGGTTTTCC TCCATTATTA ATCAGAGCAA TGACTTTTGA AATCCCTGTC 
ATTGCACCTG ATTTTCCTGT GTTAAGAAAA TATATTCTTG ATGGGGTTCA TGGAGTTTTT 
TATTCTAAAC ACAATCCTGA AGCTTTGATG AATGCTTTTT CGCTTTTGCT TTCAAGTGGA 
AGACTTTCTA AATTTGCTCA AGCTATTGGA TCATCTGGAA GACAATTTGC TAAAAATGGG 
CTTGCTTTGG ATTGCATAAT TGGCTATGCA AGACTTCTGG AGAATGTACT AAGTTTTCCT 
TCAGATTCTT TACTACCTGG TCCTGTTTCT CAAATCCAAC AAGTAGCATG GGGATGGAGT 
TTTTTCCAGA ATGAAATAGA GCTAGATATT GACTTGCTAA AAATGGATGA TGATTTTTCT 
AATGGAAAAG CTACCGTTGT CCATGCCGTT GAGAAAGAGT TGGCTAGTCT TAATTATTCA 
ACAAACTTCC TTGAGAATGG AACAGATGTT CCAATACAAG ATGAACTGAC CAAATTGGAC 
TGGGACATTC TGAGAGAAAT TGAAATATCT GAAGAAAGTG AAATGCTCGA AATTGAACAG 
GTTGAAGAGA GGTTGGAGAA AGATGTTGGT GTTTGGGATG AGATATATCG TAATGCTAGG 
AAATCTGAGA AACTGAAGTT TGAAGCCAAT GAGCGGGATG AAGGGGAGCT TGAGAGAACA 
GGACAACCTG TGTGCATTTA TGAGATATAC AGTGGTGCTG GAGTGTGGCC ATTTTTACAC 
CATGGATCTT TATATCGTGG GTTAAGCCTT TCTAGAAGAT CCCAAAGACA AAGCTCTGAT 
GATGTGGATG CTGTTGGTCG TTTGCCTCTT CTGAATGACA CATATTACCG TGACATTCTC 
TGTGAAATGG GAGGAATGTT TGCAATTGCA AATAGAGTGG ATAGTATTCA CAGGAGGCCT 
TGGATTGGGT TTCAGTCATG GCGTGCTGCT GGTAGGAAGG TAGCATTGTC AGTGGAAGCT 
GAAAGTGTTT TGGAAGAGAC AATGCATGAG AATGCTAGAG GAGATGTAAT ATATTTTTGG 
GGACGCTTGG ACCTGGACGG GGGTGCCATA GGAAGCAACA ATGCACTTAC TTTTTGGTCA 
ATGTGTGATA TCTTAAATGG AGGGAACTGC AGGAATGTTT TTCAAGATAG CTTCCGCCAG 
ATGTATTCAT TGCCCCCCCA TGCTGAAGCG CTGCCCCCTA TGCCTGAAGA TGGTGGTTAT 
TGGTCAGCAC TGCACAGTTG GGTGATGCCA ACCCCTTCTT TTCTGGAGTT CGTAATGTTC 
TCTCGGATGT TCGTTGATTC CATCGATGCT TTTCACAGAG ACTCTGGTAA ATACAGCATG 
TGCTTGTTAG GCTCTTCAGA GATTGAGGAA AAACACTGTT ACTGTCGAAT GTTGGAACTT 
CTTATCAATG TATGGGCTTA TCATAGTTCA CGAAAGATGG TATATATAAA TCCTAATACT 
GGTTCTTTGC AAGAGCAGCA CCTAGTGGAA CAACGGAAGA GTTTCATGTG GGCAAAATAT 
TTCAATTTTT CATTGTTAAA AAGTATGGAT GAAGATCTAG CAGAAGCTGC AGATGATGGT 
GACGATCCAA GGGATAAGTG GTTGTGGCCA ATGACAGGAG AAGTGCACTG GCAAGGGATT 
TATGAAAGGG AGAGGGAAGA AAGGTACAGA ATAAAAATGG ATAAAAAAAG AAAAACAAAA 
GAGAAATTAT ACGAAAGGAT GAAGTATGGT TACAAGCAGA AATCACTTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25484854 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム