LOC25483955 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25483955
Entrez Gene ID	25483955
Full Name	HASTY-like protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013612678.1	XP_013468132.1	HASTY-like protein
XM_024775012.1	XP_024630780.1	protein HASTY 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25483955 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25483955 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe211451	XM_013612678.1 Latest version!	Medicago truncatula HASTY-like protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe258765	XM_024775012.1 Latest version!	Medicago truncatula protein HASTY 1 (LOC25483955), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25483955 ( XM_013612678.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468132.1 Medicago truncatula HASTY-like protein partial mRNA.

Price & Availability↑

CloneID	OMe211451
Clone ID Related Accession (Same CDS sequence)	XM_013612678.1
Accession Version	XM_013612678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	HASTY-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAGCAA GTGGTGATAT ACGGACATTA CCGACCACAT TGTTCCTTCT GGTGAAAAAG 
AATTGGTCAT CTGAAATCCG GTTGCATGCT TTTAAGATGC TGCAGCATTT GGTGCGGTTA 
AAGTGGGAGG AATTAAGCCC GAAAGAGCAC AAAAACTTTG CGAAACTCTC CATTGATTTA 
ATGTATGAGA TTGCAGATCC TTGTGAAGAC TGGACTCTTA AAAGTCAGAC ATTGCTTCTT 
ATTCTGTCAC ATTTGCAATT TCGCTTGATA GTTAGAAGAG AGGGTCTTGT TCTATTATGG 
CATGAAGAAA TGCTTCCAAT CTTTGGTTAC TCTATCCAGC AAGGGTCCAA TACAAGTAAA 
TTTCAAGCTG AGTTGGTTTC AATGATGCTG CGGTGGCTTT CTGAGGACAT TACAGTTCAC 
AACGAGGACT TAGAAGGTGA CCGCCGAAGG CTACTTTTGC GCTGGGTAAC TCAATCATTG 
CCAGAAACTT TGCCTCTATT ACACACTTTA CTAGAAAAAC ACTGTACAGC TGCACTAAGT 
GAAGCCAGTA GAAAACAGAT CGACGTTGCC AAATTGCATG CTGCTGCAGT AACAGCTACT 
TTAAATGCAG TAATTGCATA TGCTGAGTGG GCTCCATTGA CTGATCTTGC GAAGTCTGGT 
ATCATTAATC TTTGTGGTTT CCTACTATCT GCTCCAGAAT TTCGTCTTCA TGCTTCTGAG 
TTTTTCAAAC TTGTTTCATC AAGGAAGAGA TCTGTTGATG CATCTGCTTC TGAAATTGAC 
CAAGTTATGA GGGACATCTT TCAAATATTA ATGAATATAT CTAGAGAGTT TTTGCACAAA 
TCTGGTTCAG GTCTCGGCTC CATGGATGAG AGTGAATACG ATTTTTCTGA ATGCATATGT 
GAAAGTATGG TGTCACTGGG TTCCTTTAAC TTGCAAAGTA TTGCCGGAGA TAGTGCCGTA 
TTTTCACTCT ACCTAGAACA GATGCTAGGT TTTTTCAAAA ACTACAAGTT TGCAATTCAT 
TTTCAATCCC TGCAATTCTG GCTGGTGCTA ATGAGGGATT TAACGCCAAA GCCAAGAAGT 
TCCACTCATT CAGCCTGTGA CAGTCCATCT GTTAGTTGCT CCGGTTCTGA AAATGCAAAG 
AAGAAGGACA CTTTCTTCAC ATTCTCAAAA GAGAATACAT CTCTTTTGAG TGATGATTTT 
TGTAGTGCAA TGTTGGATAC ATCTTTCCTT CATATTCTCA AAAGAGAGAA AACTCTGCCT 
GGAACTGCAC TTTCTTTAGT GGCACCAGAG TTGTGGAGTG ACGATTTTGA GGACAAGGGT 
CAATTCATCC AGTATCGTTA TAGTCTGTTG GAGCTGATTA GATTTGTTGC TTCGTACAAA 
CCCCTGATAG CTGCTGCTAA AGTTTCTGAA AAAATTGATA CAATAATCAA GAGTTTCTTG 
CTTTCACCAT CGCCGTCTCA GGACTTAGCT GCGATCGAAA GCATGCCTTT GGCTTTAGAG 
AATGTTGTAA ATGCAGTTTT CGGTGGATCC AACGATATGG CCGAGGAGAA CGCTGAAGTT 
CAGTTAGAAT TATGCAGAAC ATTTGAAGGC TTACTCCAAC AATTTATTTC CTTGAAATGG 
ACGGAGCCTG CACTTGTGGA AGTACTTGTT CACTATTTAG ATGCAATGTG TCTCTTTTTG 
AAGTATTCTC CGGATGCAGC TGGCAGTGTT ATCAATAAAC TATTTGAACT TCTAACATCT 
CTTCCACTTG AAATTAAGGA GACATCAACA TCAAGTGCTC GGCATGCAAG GTTACATATC 
TGTTCATCAT TTATTCGGAT AGCAAAAGCT ACTGACAAAA GCATCTTGCC TCATCTGAAG 
TTGTTCACAG TACTTCCCGC CGGACTAATA AGTTTGCTGT GCTATATAAC TGGTTATGAC 
GTTATGTTCA GTTGTTCACA ATATAATGTA CTGGGCTTGT CAATAACCAT TGGGGATTCA 
TTTTTCAAAA ACTTGGATGT GCACTTTGTT GCAGTTGCCC TGATGGAGAA TATACAGTCA 
ATGGAGTTTA GTCATTTGAG GGAACTTGTT CATTGCATTT TGATTCCGTT GGTTAAAAAT 
GGTCCTGTCG ATATGAGGGA GATTTGGCTG GAAAAGCTTC TGCACCCATT ATTTGATCAT 
GTTCAGCAAG CTCTTAGCTG TTCATGGTCA AGTCTTCTGC AAGATGGTAG AGCAAAGGTT 
CCTGATACTC ATGGCATTCT TAGTGGATCA GACCTGAAAG TACAGGTGAT GGAGGAAACA 
CTTTTGAGGA ATCTAACACG TCAGATTTGT TCACTCCTCT CTGTGATTGC TTCTCCTCCC 
CTAAATACTG GAATCCCTTC TTTGGAACAG TCTGGGCATA TTATTCCTTT CGACATGTCT 
TCTGTGAAAA GCTTGGATGC AGTTGCATCA TGCTCTTTGG TTGGGTTCCT GCTGAAGCAT 
GAAGGTCTTG CCCTTCCAAC GTTAAGAATG TGTTTAGAAG TTTTTAAATG GACAGATGGT 
GAAGCCGTGA CAAAGATTTC TTCTTTTTGC TCTGCGATGG TAGTTCTTTC AACAGTAACA 
AACCATACCA AACTCGTAGA ATACGTTTCT ACAGATCTTT TTACATCCGT TATTCAAGGA 
TTAGAGCTCG AGTTGAATGC AATAATCAGT GCTGATCTAG TTGAAATTTG TCGAGAGATA 
TTTGTATATC TTGGTGATAG ACACCCAGCT CCCAGGAAGG TTTTGCAGTC TCTCCCATTT 
ATTACTTCTC GTGATTTACA TGCCTTCGAG GAATCTTTGT CGAAAACGTC AAGTCCAAAG 
GAACAAAGGC AGCACATGAA AAATTTGCTT CTGATGGCAA CTGGTAACAA ATTAAAAGCA 
CTTGCAGAAC AGAAACGCAG GTTGAAACTT ACAGGAGCCC GAGACCCCGA CAACTGCTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468132.1
CDS	1..2943
Translation	MKASGDIRTLPTTLFLLVKKNWSSEIRLHAFKMLQHLVRLKWEELSPKEHKNFAKLSIDLMYEIADPCEDWTLKSQTLLLILSHLQFRLIVRREGLVLLWHEEMLPIFGYSIQQGSNTSKFQAELVSMMLRWLSEDITVHNEDLEGDRRRLLLRWVTQSLPETLPLLHTLLEKHCTAALSEASRKQIDVAKLHAAAVTATLNAVIAYAEWAPLTDLAKSGIINLCGFLLSAPEFRLHASEFFKLVSSRKRSVDASASEIDQVMRDIFQILMNISREFLHKSGSGLGSMDESEYDFSECICESMVSLGSFNLQSIAGDSAVFSLYLEQMLGFFKNYKFAIHFQSLQFWLVLMRDLTPKPRSSTHSACDSPSVSCSGSENAKKKDTFFTFSKENTSLLSDDFCSAMLDTSFLHILKREKTLPGTALSLVAPELWSDDFEDKGQFIQYRYSLLELIRFVASYKPLIAAAKVSEKIDTIIKSFLLSPSPSQDLAAIESMPLALENVVNAVFGGSNDMAEENAEVQLELCRTFEGLLQQFISLKWTEPALVEVLVHYLDAMCLFLKYSPDAAGSVINKLFELLTSLPLEIKETSTSSARHARLHICSSFIRIAKATDKSILPHLKLFTVLPAGLISLLCYITGYDVMFSCSQYNVLGLSITIGDSFFKNLDVHFVAVALMENIQSMEFSHLRELVHCILIPLVKNGPVDMREIWLEKLLHPLFDHVQQALSCSWSSLLQDGRAKVPDTHGILSGSDLKVQVMEETLLRNLTRQICSLLSVIASPPLNTGIPSLEQSGHIIPFDMSSVKSLDAVASCSLVGFLLKHEGLALPTLRMCLEVFKWTDGEAVTKISSFCSAMVVLSTVTNHTKLVEYVSTDLFTSVIQGLELELNAIISADLVEICREIFVYLGDRHPAPRKVLQSLPFITSRDLHAFEESLSKTSSPKEQRQHMKNLLLMATGNKLKALAEQKRRLKLTGARDPDNCC

Target ORF information:

RefSeq Version	XM_013612678.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula HASTY-like protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013612678.1

ORF Insert Sequence:

ATGAAAGCAA GTGGTGATAT ACGGACATTA CCGACCACAT TGTTCCTTCT GGTGAAAAAG 
AATTGGTCAT CTGAAATCCG GTTGCATGCT TTTAAGATGC TGCAGCATTT GGTGCGGTTA 
AAGTGGGAGG AATTAAGCCC GAAAGAGCAC AAAAACTTTG CGAAACTCTC CATTGATTTA 
ATGTATGAGA TTGCAGATCC TTGTGAAGAC TGGACTCTTA AAAGTCAGAC ATTGCTTCTT 
ATTCTGTCAC ATTTGCAATT TCGCTTGATA GTTAGAAGAG AGGGTCTTGT TCTATTATGG 
CATGAAGAAA TGCTTCCAAT CTTTGGTTAC TCTATCCAGC AAGGGTCCAA TACAAGTAAA 
TTTCAAGCTG AGTTGGTTTC AATGATGCTG CGGTGGCTTT CTGAGGACAT TACAGTTCAC 
AACGAGGACT TAGAAGGTGA CCGCCGAAGG CTACTTTTGC GCTGGGTAAC TCAATCATTG 
CCAGAAACTT TGCCTCTATT ACACACTTTA CTAGAAAAAC ACTGTACAGC TGCACTAAGT 
GAAGCCAGTA GAAAACAGAT CGACGTTGCC AAATTGCATG CTGCTGCAGT AACAGCTACT 
TTAAATGCAG TAATTGCATA TGCTGAGTGG GCTCCATTGA CTGATCTTGC GAAGTCTGGT 
ATCATTAATC TTTGTGGTTT CCTACTATCT GCTCCAGAAT TTCGTCTTCA TGCTTCTGAG 
TTTTTCAAAC TTGTTTCATC AAGGAAGAGA TCTGTTGATG CATCTGCTTC TGAAATTGAC 
CAAGTTATGA GGGACATCTT TCAAATATTA ATGAATATAT CTAGAGAGTT TTTGCACAAA 
TCTGGTTCAG GTCTCGGCTC CATGGATGAG AGTGAATACG ATTTTTCTGA ATGCATATGT 
GAAAGTATGG TGTCACTGGG TTCCTTTAAC TTGCAAAGTA TTGCCGGAGA TAGTGCCGTA 
TTTTCACTCT ACCTAGAACA GATGCTAGGT TTTTTCAAAA ACTACAAGTT TGCAATTCAT 
TTTCAATCCC TGCAATTCTG GCTGGTGCTA ATGAGGGATT TAACGCCAAA GCCAAGAAGT 
TCCACTCATT CAGCCTGTGA CAGTCCATCT GTTAGTTGCT CCGGTTCTGA AAATGCAAAG 
AAGAAGGACA CTTTCTTCAC ATTCTCAAAA GAGAATACAT CTCTTTTGAG TGATGATTTT 
TGTAGTGCAA TGTTGGATAC ATCTTTCCTT CATATTCTCA AAAGAGAGAA AACTCTGCCT 
GGAACTGCAC TTTCTTTAGT GGCACCAGAG TTGTGGAGTG ACGATTTTGA GGACAAGGGT 
CAATTCATCC AGTATCGTTA TAGTCTGTTG GAGCTGATTA GATTTGTTGC TTCGTACAAA 
CCCCTGATAG CTGCTGCTAA AGTTTCTGAA AAAATTGATA CAATAATCAA GAGTTTCTTG 
CTTTCACCAT CGCCGTCTCA GGACTTAGCT GCGATCGAAA GCATGCCTTT GGCTTTAGAG 
AATGTTGTAA ATGCAGTTTT CGGTGGATCC AACGATATGG CCGAGGAGAA CGCTGAAGTT 
CAGTTAGAAT TATGCAGAAC ATTTGAAGGC TTACTCCAAC AATTTATTTC CTTGAAATGG 
ACGGAGCCTG CACTTGTGGA AGTACTTGTT CACTATTTAG ATGCAATGTG TCTCTTTTTG 
AAGTATTCTC CGGATGCAGC TGGCAGTGTT ATCAATAAAC TATTTGAACT TCTAACATCT 
CTTCCACTTG AAATTAAGGA GACATCAACA TCAAGTGCTC GGCATGCAAG GTTACATATC 
TGTTCATCAT TTATTCGGAT AGCAAAAGCT ACTGACAAAA GCATCTTGCC TCATCTGAAG 
TTGTTCACAG TACTTCCCGC CGGACTAATA AGTTTGCTGT GCTATATAAC TGGTTATGAC 
GTTATGTTCA GTTGTTCACA ATATAATGTA CTGGGCTTGT CAATAACCAT TGGGGATTCA 
TTTTTCAAAA ACTTGGATGT GCACTTTGTT GCAGTTGCCC TGATGGAGAA TATACAGTCA 
ATGGAGTTTA GTCATTTGAG GGAACTTGTT CATTGCATTT TGATTCCGTT GGTTAAAAAT 
GGTCCTGTCG ATATGAGGGA GATTTGGCTG GAAAAGCTTC TGCACCCATT ATTTGATCAT 
GTTCAGCAAG CTCTTAGCTG TTCATGGTCA AGTCTTCTGC AAGATGGTAG AGCAAAGGTT 
CCTGATACTC ATGGCATTCT TAGTGGATCA GACCTGAAAG TACAGGTGAT GGAGGAAACA 
CTTTTGAGGA ATCTAACACG TCAGATTTGT TCACTCCTCT CTGTGATTGC TTCTCCTCCC 
CTAAATACTG GAATCCCTTC TTTGGAACAG TCTGGGCATA TTATTCCTTT CGACATGTCT 
TCTGTGAAAA GCTTGGATGC AGTTGCATCA TGCTCTTTGG TTGGGTTCCT GCTGAAGCAT 
GAAGGTCTTG CCCTTCCAAC GTTAAGAATG TGTTTAGAAG TTTTTAAATG GACAGATGGT 
GAAGCCGTGA CAAAGATTTC TTCTTTTTGC TCTGCGATGG TAGTTCTTTC AACAGTAACA 
AACCATACCA AACTCGTAGA ATACGTTTCT ACAGATCTTT TTACATCCGT TATTCAAGGA 
TTAGAGCTCG AGTTGAATGC AATAATCAGT GCTGATCTAG TTGAAATTTG TCGAGAGATA 
TTTGTATATC TTGGTGATAG ACACCCAGCT CCCAGGAAGG TTTTGCAGTC TCTCCCATTT 
ATTACTTCTC GTGATTTACA TGCCTTCGAG GAATCTTTGT CGAAAACGTC AAGTCCAAAG 
GAACAAAGGC AGCACATGAA AAATTTGCTT CTGATGGCAA CTGGTAACAA ATTAAAAGCA 
CTTGCAGAAC AGAAACGCAG GTTGAAACTT ACAGGAGCCC GAGACCCCGA CAACTGCTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25483955 ( XM_024775012.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024630780.1 Medicago truncatula protein HASTY 1 (LOC25483955), mRNA.

Price & Availability↑

CloneID	OMe258765
Clone ID Related Accession (Same CDS sequence)	XM_024775012.1
Accession Version	XM_024775012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	protein HASTY 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases ##RefSeq-Attributes-END##

ATGGTAGATA ATTTCAAAGT TTGCTTCACT GTTATTTCTA ATGTTGTGCA AGTTGATAGA 
TTCATCGGTT GGAACAGTGC TAGCTATTCT TGTGCCATCG CTAATGTTGA TGGTAGTTGT 
CTCAGCACCT CTGTTAGAGC TGGTTATGCT GGAATCATAA GAAATAGTGC AGACTTTTTA 
TTAATCAAGG TTTTCAGGGT CTCATATTGG CAAGAAGTTT GGACATTGAA GAGCTCATTT 
GCTACTCCAA CTCTTTACTC GATGAAAGCA AGTGGTGATA TACGGACATT ACCGACCACA 
TTGTTCCTTC TGGTGAAAAA GAATTGGTCA TCTGAAATCC GGTTGCATGC TTTTAAGATG 
CTGCAGCATT TGGTGCGGTT AAAGTGGGAG GAATTAAGCC CGAAAGAGCA CAAAAACTTT 
GCGAAACTCT CCATTGATTT AATGTATGAG ATTGCAGATC CTTGTGAAGA CTGGACTCTT 
AAAAGTCAGA CATTGCTTCT TATTCTGGTC TTGTTCTATT ATGGCATGAA GAAATGCTTC 
CAATCTTTGG TTACTCTATC CAGCAAGGGT CCAATACAAG CTGAGTTGGT TTCAATGATG 
CTGCGGTGGC TTTCTGAGGA CATTACAGTT CACAACGAGG ACTTAGAAGG TGACCGCCGA 
AGGCTACTTT TGCGCTGGGT AACTCAATCA TTGCCAGAAA CTTTGCCTCT ATTACACACT 
TTACTAGAAA AACACTGTAC AGCTGCACTA AGTGAAGCCA GTAGAAAACA GATCGACGTT 
GCCAAATTGC ATGCTGCTGC AGTAACAGCT ACTTTAAATG CAGTAATTGC ATATGCTGAG 
TGGGCTCCAT TGACTGATCT TGCGAAGTCT GGTATCATTA ATCTTTGTGG TTTCCTACTA 
TCTGCTCCAG AATTTCGTCT TCATGCTTCT GAGTTTTTCA AACTTGTTTC ATCAAGGAAG 
AGATCTGTTG ATGCATCTGC TTCTGAAATT GACCAAGTTA TGAGGGACAT CTTTCAAATA 
TTAATGAATA TATCTAGAGA GTTTTTGCAC AAATCTGGTT CAGGTCTCGG CTCCATGGAT 
GAGAGTGAAT ACGATTTTTC TGAATGCATA TGTGAAAGTA TGGTGTCACT GGGTTCCTTT 
AACTTGCAAA GTATTGCCGG AGATAGTGCC GTATTTTCAC TCTACCTAGA ACAGATGCTA 
GGTTTTTTCA AAAACTACAA GTTTGCAATT CATTTTCAAT CCCTGCAATT CTGGCTGGTG 
CTAATGAGGG ATTTAACGCC AAAGCCAAGA AGTTCCACTC ATTCAGCCTG TGACAGTCCA 
TCTGTTAGTT GCTCCGGTTC TGAAAATGCA AAGAAGAAGG ACACTTTCTT CACATTCTCA 
AAAGAGAATA CATCTCTTTT GAGTGATGAT TTTTGTAGTG CAATGTTGGA TACATCTTTC 
CTTCATATTC TCAAAAGAGA GAAAACTCTG CCTGGAACTG CACTTTCTTT AGTGGCACCA 
GAGTTGTGGA GTGACGATTT TGAGGACAAG GGTCAATTCA TCCAGTATCG TTATAGTCTG 
TTGGAGCTGA TTAGATTTGT TGCTTCGTAC AAACCCCTGA TAGCTGCTGC TAAAGTTTCT 
GAAAAAATTG ATACAATAAT CAAGAGTTTC TTGCTTTCAC CATCGCCGTC TCAGGACTTA 
GCTGCGATCG AAAGCATGCC TTTGGCTTTA GAGAATGTTG TAAATGCAGT TTTCGGTGGA 
TCCAACGATA TGGCCGAGGA GAACGCTGAA GTTCAGTTAG AATTATGCAG AACATTTGAA 
GGCTTACTCC AACAATTTAT TTCCTTGAAA TGGACGGAGC CTGCACTTGT GGAAGTACTT 
GTTCACTATT TAGATGCAAT GTGTCTCTTT TTGAAGTATT CTCCGGATGC AGCTGGCAGT 
GTTATCAATA AACTATTTGA ACTTCTAACA TCTCTTCCAC TTGAAATTAA GGAGACATCA 
ACATCAAGTG CTCGGCATGC AAGGTTACAT ATCTGTTCAT CATTTATTCG GATAGCAAAA 
GCTACTGACA AAAGCATCTT GCCTCATCTG AAGGTGCATT TGTTTAAACA TCCGTTGTTG 
AACGATCACA TACTTCCCGC CGGACTAATA AGTTTGCTGT GCTATATAAC TGGTTATGAC 
GTTATGTTCA GTTGTTCACA ATATAATGTA CTGGGCTTGT CAATAACCAT TGGGGATTCA 
TTTTTCAAAA ACTTGGATGT GCACTTTGTT GCAGTTGCCC TGATGGAGAA TATACAGTCA 
ATGGAGTTTA GTCATTTGAG GGAACTTGTT CATTGCATTT TGATTCCGTT GGTTAAAAAT 
GGTCCTGTCG ATATGAGGGA GATTTGGCTG GAAAAGCTTC TGCACCCATT ATTTGATCAT 
GTTCAGCAAG CTCTTAGCTG TTCATGGTCA AGTCTTCTGC AAGATGGTAG AGCAAAGGTT 
CCTGATACTC ATGGCATTCT TAGTGGATCA GACCTGAAAG TACAGGTGAT GGAGGAAACA 
CTTTTGAGGA ATCTAACACG TCAGATTTGT TCACTCCTCT CTGTGATTGC TTCTCCTCCC 
CTAAATACTG GAATCCCTTC TTTGGAACAG TCTGGGCATA TTATTCCTTT CGACATGTCT 
TCTGTGAAAA GCTTGGATGC AGTTGCATCA TGCTCTTTGG TTGGGTTCCT GCTGAAGCAT 
GAAGGTCTTG CCCTTCCAAC GTTAAGAATG TGTTTAGAAG TTTTTAAATG GACAGATGGT 
GAAGCCGTGA CAAAGATTTC TTCTTTTTGC TCTGCGATGG TAGTTCTTTC AACAGTAACA 
AACCATACCA AACTCGTAGA ATACGTTTCT ACAGATCTTT TTACATCCGT TATTCAAGGA 
TTAGAGCTCG AGTTGAATGC AATAATCAGT GCTGATCTAG TTGAAATTTG TCGAGAGATA 
TTTGTATATC TTGGTGATAG ACACCCAGCT CCCAGGAAGG TTTTGCAGTC TCTCCCATTT 
ATTACTTCTC GTGATTTACA TGCCTTCGAG GAATCTTTGT CGAAAACGTC AAGTCCAAAG 
GAACAAAGGC AGCACATGAA AAATTTGCTT CTGATGGCAA CTGGTAACAA ATTAAAAGCA 
CTTGCAGAAC AGAAACGCAG GTTGAAACTT ACAGGAGCCC GAGACCCCGA CAACTGCTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024630780.1
CDS	1..3183
Translation	MVDNFKVCFTVISNVVQVDRFIGWNSASYSCAIANVDGSCLSTSVRAGYAGIIRNSADFLLIKVFRVSYWQEVWTLKSSFATPTLYSMKASGDIRTLPTTLFLLVKKNWSSEIRLHAFKMLQHLVRLKWEELSPKEHKNFAKLSIDLMYEIADPCEDWTLKSQTLLLILVLFYYGMKKCFQSLVTLSSKGPIQAELVSMMLRWLSEDITVHNEDLEGDRRRLLLRWVTQSLPETLPLLHTLLEKHCTAALSEASRKQIDVAKLHAAAVTATLNAVIAYAEWAPLTDLAKSGIINLCGFLLSAPEFRLHASEFFKLVSSRKRSVDASASEIDQVMRDIFQILMNISREFLHKSGSGLGSMDESEYDFSECICESMVSLGSFNLQSIAGDSAVFSLYLEQMLGFFKNYKFAIHFQSLQFWLVLMRDLTPKPRSSTHSACDSPSVSCSGSENAKKKDTFFTFSKENTSLLSDDFCSAMLDTSFLHILKREKTLPGTALSLVAPELWSDDFEDKGQFIQYRYSLLELIRFVASYKPLIAAAKVSEKIDTIIKSFLLSPSPSQDLAAIESMPLALENVVNAVFGGSNDMAEENAEVQLELCRTFEGLLQQFISLKWTEPALVEVLVHYLDAMCLFLKYSPDAAGSVINKLFELLTSLPLEIKETSTSSARHARLHICSSFIRIAKATDKSILPHLKVHLFKHPLLNDHILPAGLISLLCYITGYDVMFSCSQYNVLGLSITIGDSFFKNLDVHFVAVALMENIQSMEFSHLRELVHCILIPLVKNGPVDMREIWLEKLLHPLFDHVQQALSCSWSSLLQDGRAKVPDTHGILSGSDLKVQVMEETLLRNLTRQICSLLSVIASPPLNTGIPSLEQSGHIIPFDMSSVKSLDAVASCSLVGFLLKHEGLALPTLRMCLEVFKWTDGEAVTKISSFCSAMVVLSTVTNHTKLVEYVSTDLFTSVIQGLELELNAIISADLVEICREIFVYLGDRHPAPRKVLQSLPFITSRDLHAFEESLSKTSSPKEQRQHMKNLLLMATGNKLKALAEQKRRLKLTGARDPDNCC

Target ORF information:

RefSeq Version	XM_024775012.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula protein HASTY 1 (LOC25483955), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024775012.1

ORF Insert Sequence:

ATGGTAGATA ATTTCAAAGT TTGCTTCACT GTTATTTCTA ATGTTGTGCA AGTTGATAGA 
TTCATCGGTT GGAACAGTGC TAGCTATTCT TGTGCCATCG CTAATGTTGA TGGTAGTTGT 
CTCAGCACCT CTGTTAGAGC TGGTTATGCT GGAATCATAA GAAATAGTGC AGACTTTTTA 
TTAATCAAGG TTTTCAGGGT CTCATATTGG CAAGAAGTTT GGACATTGAA GAGCTCATTT 
GCTACTCCAA CTCTTTACTC GATGAAAGCA AGTGGTGATA TACGGACATT ACCGACCACA 
TTGTTCCTTC TGGTGAAAAA GAATTGGTCA TCTGAAATCC GGTTGCATGC TTTTAAGATG 
CTGCAGCATT TGGTGCGGTT AAAGTGGGAG GAATTAAGCC CGAAAGAGCA CAAAAACTTT 
GCGAAACTCT CCATTGATTT AATGTATGAG ATTGCAGATC CTTGTGAAGA CTGGACTCTT 
AAAAGTCAGA CATTGCTTCT TATTCTGGTC TTGTTCTATT ATGGCATGAA GAAATGCTTC 
CAATCTTTGG TTACTCTATC CAGCAAGGGT CCAATACAAG CTGAGTTGGT TTCAATGATG 
CTGCGGTGGC TTTCTGAGGA CATTACAGTT CACAACGAGG ACTTAGAAGG TGACCGCCGA 
AGGCTACTTT TGCGCTGGGT AACTCAATCA TTGCCAGAAA CTTTGCCTCT ATTACACACT 
TTACTAGAAA AACACTGTAC AGCTGCACTA AGTGAAGCCA GTAGAAAACA GATCGACGTT 
GCCAAATTGC ATGCTGCTGC AGTAACAGCT ACTTTAAATG CAGTAATTGC ATATGCTGAG 
TGGGCTCCAT TGACTGATCT TGCGAAGTCT GGTATCATTA ATCTTTGTGG TTTCCTACTA 
TCTGCTCCAG AATTTCGTCT TCATGCTTCT GAGTTTTTCA AACTTGTTTC ATCAAGGAAG 
AGATCTGTTG ATGCATCTGC TTCTGAAATT GACCAAGTTA TGAGGGACAT CTTTCAAATA 
TTAATGAATA TATCTAGAGA GTTTTTGCAC AAATCTGGTT CAGGTCTCGG CTCCATGGAT 
GAGAGTGAAT ACGATTTTTC TGAATGCATA TGTGAAAGTA TGGTGTCACT GGGTTCCTTT 
AACTTGCAAA GTATTGCCGG AGATAGTGCC GTATTTTCAC TCTACCTAGA ACAGATGCTA 
GGTTTTTTCA AAAACTACAA GTTTGCAATT CATTTTCAAT CCCTGCAATT CTGGCTGGTG 
CTAATGAGGG ATTTAACGCC AAAGCCAAGA AGTTCCACTC ATTCAGCCTG TGACAGTCCA 
TCTGTTAGTT GCTCCGGTTC TGAAAATGCA AAGAAGAAGG ACACTTTCTT CACATTCTCA 
AAAGAGAATA CATCTCTTTT GAGTGATGAT TTTTGTAGTG CAATGTTGGA TACATCTTTC 
CTTCATATTC TCAAAAGAGA GAAAACTCTG CCTGGAACTG CACTTTCTTT AGTGGCACCA 
GAGTTGTGGA GTGACGATTT TGAGGACAAG GGTCAATTCA TCCAGTATCG TTATAGTCTG 
TTGGAGCTGA TTAGATTTGT TGCTTCGTAC AAACCCCTGA TAGCTGCTGC TAAAGTTTCT 
GAAAAAATTG ATACAATAAT CAAGAGTTTC TTGCTTTCAC CATCGCCGTC TCAGGACTTA 
GCTGCGATCG AAAGCATGCC TTTGGCTTTA GAGAATGTTG TAAATGCAGT TTTCGGTGGA 
TCCAACGATA TGGCCGAGGA GAACGCTGAA GTTCAGTTAG AATTATGCAG AACATTTGAA 
GGCTTACTCC AACAATTTAT TTCCTTGAAA TGGACGGAGC CTGCACTTGT GGAAGTACTT 
GTTCACTATT TAGATGCAAT GTGTCTCTTT TTGAAGTATT CTCCGGATGC AGCTGGCAGT 
GTTATCAATA AACTATTTGA ACTTCTAACA TCTCTTCCAC TTGAAATTAA GGAGACATCA 
ACATCAAGTG CTCGGCATGC AAGGTTACAT ATCTGTTCAT CATTTATTCG GATAGCAAAA 
GCTACTGACA AAAGCATCTT GCCTCATCTG AAGGTGCATT TGTTTAAACA TCCGTTGTTG 
AACGATCACA TACTTCCCGC CGGACTAATA AGTTTGCTGT GCTATATAAC TGGTTATGAC 
GTTATGTTCA GTTGTTCACA ATATAATGTA CTGGGCTTGT CAATAACCAT TGGGGATTCA 
TTTTTCAAAA ACTTGGATGT GCACTTTGTT GCAGTTGCCC TGATGGAGAA TATACAGTCA 
ATGGAGTTTA GTCATTTGAG GGAACTTGTT CATTGCATTT TGATTCCGTT GGTTAAAAAT 
GGTCCTGTCG ATATGAGGGA GATTTGGCTG GAAAAGCTTC TGCACCCATT ATTTGATCAT 
GTTCAGCAAG CTCTTAGCTG TTCATGGTCA AGTCTTCTGC AAGATGGTAG AGCAAAGGTT 
CCTGATACTC ATGGCATTCT TAGTGGATCA GACCTGAAAG TACAGGTGAT GGAGGAAACA 
CTTTTGAGGA ATCTAACACG TCAGATTTGT TCACTCCTCT CTGTGATTGC TTCTCCTCCC 
CTAAATACTG GAATCCCTTC TTTGGAACAG TCTGGGCATA TTATTCCTTT CGACATGTCT 
TCTGTGAAAA GCTTGGATGC AGTTGCATCA TGCTCTTTGG TTGGGTTCCT GCTGAAGCAT 
GAAGGTCTTG CCCTTCCAAC GTTAAGAATG TGTTTAGAAG TTTTTAAATG GACAGATGGT 
GAAGCCGTGA CAAAGATTTC TTCTTTTTGC TCTGCGATGG TAGTTCTTTC AACAGTAACA 
AACCATACCA AACTCGTAGA ATACGTTTCT ACAGATCTTT TTACATCCGT TATTCAAGGA 
TTAGAGCTCG AGTTGAATGC AATAATCAGT GCTGATCTAG TTGAAATTTG TCGAGAGATA 
TTTGTATATC TTGGTGATAG ACACCCAGCT CCCAGGAAGG TTTTGCAGTC TCTCCCATTT 
ATTACTTCTC GTGATTTACA TGCCTTCGAG GAATCTTTGT CGAAAACGTC AAGTCCAAAG 
GAACAAAGGC AGCACATGAA AAATTTGCTT CTGATGGCAA CTGGTAACAA ATTAAAAGCA 
CTTGCAGAAC AGAAACGCAG GTTGAAACTT ACAGGAGCCC GAGACCCCGA CAACTGCTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25483955 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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