LOC11446024 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11446024
Entrez Gene ID	11446024
Full Name	sn1-specific diacylglycerol lipase beta-like protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013615057.1	XP_013470511.1	sn1-specific diacylglycerol lipase beta-like protein
XM_003592800.2	XP_003592848.2	sn1-specific diacylglycerol lipase beta-like protein
XM_003592800.3	XP_003592848.2	uncharacterized protein LOC11446024

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11446024 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11446024 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe208304	XM_003592800.3 Latest version!	Medicago truncatula uncharacterized LOC11446024 (LOC11446024), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe213876	XM_013615057.1 Latest version!	Medicago truncatula sn1-specific diacylglycerol lipase beta-like protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OMe208304	XM_003592800.2	Medicago truncatula sn1-specific diacylglycerol lipase beta-like protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11446024 ( XM_003592800.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003592848.2 Medicago truncatula sn1-specific diacylglycerol lipase beta-like protein mRNA.

Price & Availability↑

CloneID	OMe208304
Clone ID Related Accession (Same CDS sequence)	XM_003592800.2 , XM_003592800.3
Accession Version	XM_003592800.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	sn1-specific diacylglycerol lipase beta-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). On Aug 25, 2015 this sequence version replaced XM_003592800.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGAAATGGG AGGAGGCGGA AGGCAGGGAG CAGAGGAAAA TGGGTGTTGG AAACGTTAGA 
CGTATGGGGA TTGTTTTAGG GATTTGTAAC GCACTGGTTT TCCTCCTTGG TTCCCTTCTC 
ATTTCCCACA ATTATCGCCT CTGCGATCTC CGTTCTCTTC TCCCTTTCGT TGCCGTTTCA 
TTCTCTGCCG TTGTAAGGAT TCTTGTTATG CTTCAAACCG CTTCTGCTCA ACAATCTGCT 
GCAACTCTCA TTCTTCTAGA TGATCACAAC CACGAAGATC ACCTTCTTCT TCGCTTCCAA 
AGACGGGCGA GGTATAAAAT ATGGCTTTGG TGGAGTCGAT GCACCGTCGC CCTCACTATT 
ATTCAATTTG TGTGTGCTGC TTACCTTATC CTCAACTCTG TTTACTATTT ATCCAAAGAT 
AACACCACAC CAACTGCTTG TATTTTAGAA TTAGGATGGA ACCCTCACTG GTGGAACCAC 
AAGCTACTCT CTCTATTCAT CATCCTTCTC TCTTTCGTTG CTCTAGCTCA ATGCTTTACT 
GGATCCGATG TTCTCCGATG GAGATCTTTC TATGAATCCC ACGACAATGC TTGGAAATCC 
CATTACAGGG AAGTTTTTGA TAACGGCTTA CGCGAGACCT TGTGCTGTCT CGGCCGCGTC 
AAATACTTGA CTGCAATCCA ACAAGAAGAA GATGAAGTCT ATTCCGTTGC ACGCTTATTA 
GGCGATCTTG TTGCTTATCG TGCTTCTGGA ACTGGCCATA TGGAACTCTT GGCAGCTTTA 
GCTTTACTCC AAAGCAATGA AAAATCTTCA GAATCTTGTC AAGTATCCAT GGAAGCACCA 
GAGATGCGAA TCAGGGAGGC TGCAGCTCTT CATAAATTTG CTGAAGCTGC ATACACTGGT 
CCGTTGCTTG ATGTTGGACG AAATCCTCTC ATGTTTCCTT GTTCATGGCT CTATAGACAA 
GGGGTTCTGT CTCCTTGGGC ACGCAATAGA CGACCTGTTT TGGATGGTGA TAACTGGTGG 
CGAGGCCATG CTGCAGCCTT TCTAAAGTAT GCCAATTTGC CTCCGGAAGC GCTCAGACAT 
GGGAGAGTAA ACCAGGTCAA GTGTGAAGCT GCATATTTTA TTGTGGTTCT ACATCATCTA 
CAAACTGTAG TAATTGCTAT ACGTGGAACT GAAACCCCTG AGGACCTAAT AACCGATGGA 
TTATGCAAGG AATGCACCCT TTCTGCAGAA GACTTGGCTG GTTTGATAAA CTGCAACCAC 
ATTCATTCTG ATATACACAA AAATGTGGCT TCATCATTCC CCCACTATGG ACATTCGGGT 
ATAGTCGAGG CTGCACGAGA GCTTTATATG CAGATTGAAG GAAACCCTGG AGAGCATGAC 
ACTGAATCCT ATGGGCTTCT TTCTAAATTA CTGGGATTTG GATGTGAATG CTTTGGATAT 
AATGTCCGCA TCGTTGGACA TTCACTTGGG GGGGCTATAG CTGCATTGCT TGGACTGCAA 
CTTTATAACC GATACCCTAA TCTGCACGTT TACTCATATG GGCCACTCCC ATGTGTGGAT 
TTAGTTGTGG CTAATGCATG CTCATCATTT ATAACAAGCA TTATATATGG AAATGAGTTT 
TCATCCCGCC TTTCATTTGG ATCCATTATG AGGCTAAGGG CAGCTGCAAT CACATTACTG 
GCACAAGATC CTAAAGCTGA CAGTGCCATG ATTTTCAGAC TTGCTCGCCG GTTTCTATAT 
ATAAGCCAAT ATGAAAGAAA TAATCAAGAG GCAGAGCATG AAGCACAGTG TGACATTGAT 
AATAAAGGCA GCAACATACA AGTGGTTCAA GAAGCTTCAC TCTTGACTGA GGCAATTAAA 
AGGGATCTTC TTGTTACAGC TGACCATGGC AACAATGACC ATGATGACTT TTCACTCTGG 
GCTGATACCA GGGCAAGGGA CCATATTGTT GAAATTAATA ATGCCGAATT TACAAATCCT 
TTTGCTACAA ATGTACATTC AATAGATGAT CCGGTGTCTC AGTTTATAGA TTCTGTTCCA 
ACTTCTGAAA ATCAGTCTGC TGATGATCCC CCTGAGATGT ATCTGCCAGG ATTTGTAATT 
CATATTGTCC CAGACCAGAA AAGACCTCAA ACTGATTTTA AGATATCTTG GAGAACACAG 
GAGAGGGGGA AATGTTATAG AGCTTATGTA GCAAATAGAG AAAGCTTCAA AGATATTATT 
GTCTCACCAT CCATGTTCCT TGATCATCTC CCTTGGAGAT GTCATAATGC CTTGAAAAAA 
ATATTGAAAG ATCTAACTGC TAAAGATCAA GTAATGGAAC ACAACTTAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003592848.2
CDS	91..2424
Translation	MKWEEAEGREQRKMGVGNVRRMGIVLGICNALVFLLGSLLISHNYRLCDLRSLLPFVAVSFSAVVRILVMLQTASAQQSAATLILLDDHNHEDHLLLRFQRRARYKIWLWWSRCTVALTIIQFVCAAYLILNSVYYLSKDNTTPTACILELGWNPHWWNHKLLSLFIILLSFVALAQCFTGSDVLRWRSFYESHDNAWKSHYREVFDNGLRETLCCLGRVKYLTAIQQEEDEVYSVARLLGDLVAYRASGTGHMELLAALALLQSNEKSSESCQVSMEAPEMRIREAAALHKFAEAAYTGPLLDVGRNPLMFPCSWLYRQGVLSPWARNRRPVLDGDNWWRGHAAAFLKYANLPPEALRHGRVNQVKCEAAYFIVVLHHLQTVVIAIRGTETPEDLITDGLCKECTLSAEDLAGLINCNHIHSDIHKNVASSFPHYGHSGIVEAARELYMQIEGNPGEHDTESYGLLSKLLGFGCECFGYNVRIVGHSLGGAIAALLGLQLYNRYPNLHVYSYGPLPCVDLVVANACSSFITSIIYGNEFSSRLSFGSIMRLRAAAITLLAQDPKADSAMIFRLARRFLYISQYERNNQEAEHEAQCDIDNKGSNIQVVQEASLLTEAIKRDLLVTADHGNNDHDDFSLWADTRARDHIVEINNAEFTNPFATNVHSIDDPVSQFIDSVPTSENQSADDPPEMYLPGFVIHIVPDQKRPQTDFKISWRTQERGKCYRAYVANRESFKDIIVSPSMFLDHLPWRCHNALKKILKDLTAKDQVMEHNLI

Target ORF information:

RefSeq Version	XM_003592800.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula sn1-specific diacylglycerol lipase beta-like protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003592800.2

ORF Insert Sequence:

ATGAAATGGG AGGAGGCGGA AGGCAGGGAG CAGAGGAAAA TGGGTGTTGG AAACGTTAGA 
CGTATGGGGA TTGTTTTAGG GATTTGTAAC GCACTGGTTT TCCTCCTTGG TTCCCTTCTC 
ATTTCCCACA ATTATCGCCT CTGCGATCTC CGTTCTCTTC TCCCTTTCGT TGCCGTTTCA 
TTCTCTGCCG TTGTAAGGAT TCTTGTTATG CTTCAAACCG CTTCTGCTCA ACAATCTGCT 
GCAACTCTCA TTCTTCTAGA TGATCACAAC CACGAAGATC ACCTTCTTCT TCGCTTCCAA 
AGACGGGCGA GGTATAAAAT ATGGCTTTGG TGGAGTCGAT GCACCGTCGC CCTCACTATT 
ATTCAATTTG TGTGTGCTGC TTACCTTATC CTCAACTCTG TTTACTATTT ATCCAAAGAT 
AACACCACAC CAACTGCTTG TATTTTAGAA TTAGGATGGA ACCCTCACTG GTGGAACCAC 
AAGCTACTCT CTCTATTCAT CATCCTTCTC TCTTTCGTTG CTCTAGCTCA ATGCTTTACT 
GGATCCGATG TTCTCCGATG GAGATCTTTC TATGAATCCC ACGACAATGC TTGGAAATCC 
CATTACAGGG AAGTTTTTGA TAACGGCTTA CGCGAGACCT TGTGCTGTCT CGGCCGCGTC 
AAATACTTGA CTGCAATCCA ACAAGAAGAA GATGAAGTCT ATTCCGTTGC ACGCTTATTA 
GGCGATCTTG TTGCTTATCG TGCTTCTGGA ACTGGCCATA TGGAACTCTT GGCAGCTTTA 
GCTTTACTCC AAAGCAATGA AAAATCTTCA GAATCTTGTC AAGTATCCAT GGAAGCACCA 
GAGATGCGAA TCAGGGAGGC TGCAGCTCTT CATAAATTTG CTGAAGCTGC ATACACTGGT 
CCGTTGCTTG ATGTTGGACG AAATCCTCTC ATGTTTCCTT GTTCATGGCT CTATAGACAA 
GGGGTTCTGT CTCCTTGGGC ACGCAATAGA CGACCTGTTT TGGATGGTGA TAACTGGTGG 
CGAGGCCATG CTGCAGCCTT TCTAAAGTAT GCCAATTTGC CTCCGGAAGC GCTCAGACAT 
GGGAGAGTAA ACCAGGTCAA GTGTGAAGCT GCATATTTTA TTGTGGTTCT ACATCATCTA 
CAAACTGTAG TAATTGCTAT ACGTGGAACT GAAACCCCTG AGGACCTAAT AACCGATGGA 
TTATGCAAGG AATGCACCCT TTCTGCAGAA GACTTGGCTG GTTTGATAAA CTGCAACCAC 
ATTCATTCTG ATATACACAA AAATGTGGCT TCATCATTCC CCCACTATGG ACATTCGGGT 
ATAGTCGAGG CTGCACGAGA GCTTTATATG CAGATTGAAG GAAACCCTGG AGAGCATGAC 
ACTGAATCCT ATGGGCTTCT TTCTAAATTA CTGGGATTTG GATGTGAATG CTTTGGATAT 
AATGTCCGCA TCGTTGGACA TTCACTTGGG GGGGCTATAG CTGCATTGCT TGGACTGCAA 
CTTTATAACC GATACCCTAA TCTGCACGTT TACTCATATG GGCCACTCCC ATGTGTGGAT 
TTAGTTGTGG CTAATGCATG CTCATCATTT ATAACAAGCA TTATATATGG AAATGAGTTT 
TCATCCCGCC TTTCATTTGG ATCCATTATG AGGCTAAGGG CAGCTGCAAT CACATTACTG 
GCACAAGATC CTAAAGCTGA CAGTGCCATG ATTTTCAGAC TTGCTCGCCG GTTTCTATAT 
ATAAGCCAAT ATGAAAGAAA TAATCAAGAG GCAGAGCATG AAGCACAGTG TGACATTGAT 
AATAAAGGCA GCAACATACA AGTGGTTCAA GAAGCTTCAC TCTTGACTGA GGCAATTAAA 
AGGGATCTTC TTGTTACAGC TGACCATGGC AACAATGACC ATGATGACTT TTCACTCTGG 
GCTGATACCA GGGCAAGGGA CCATATTGTT GAAATTAATA ATGCCGAATT TACAAATCCT 
TTTGCTACAA ATGTACATTC AATAGATGAT CCGGTGTCTC AGTTTATAGA TTCTGTTCCA 
ACTTCTGAAA ATCAGTCTGC TGATGATCCC CCTGAGATGT ATCTGCCAGG ATTTGTAATT 
CATATTGTCC CAGACCAGAA AAGACCTCAA ACTGATTTTA AGATATCTTG GAGAACACAG 
GAGAGGGGGA AATGTTATAG AGCTTATGTA GCAAATAGAG AAAGCTTCAA AGATATTATT 
GTCTCACCAT CCATGTTCCT TGATCATCTC CCTTGGAGAT GTCATAATGC CTTGAAAAAA 
ATATTGAAAG ATCTAACTGC TAAAGATCAA GTAATGGAAC ACAACTTAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11446024 ( XM_003592800.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003592848.2 Medicago truncatula uncharacterized LOC11446024 (LOC11446024), mRNA.

Price & Availability↑

CloneID	OMe208304
Clone ID Related Accession (Same CDS sequence)	XM_003592800.2 , XM_003592800.3
Accession Version	XM_003592800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	uncharacterized protein LOC11446024
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003592800.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGG AGGAGGCGGA AGGCAGGGAG CAGAGGAAAA TGGGTGTTGG AAACGTTAGA 
CGTATGGGGA TTGTTTTAGG GATTTGTAAC GCACTGGTTT TCCTCCTTGG TTCCCTTCTC 
ATTTCCCACA ATTATCGCCT CTGCGATCTC CGTTCTCTTC TCCCTTTCGT TGCCGTTTCA 
TTCTCTGCCG TTGTAAGGAT TCTTGTTATG CTTCAAACCG CTTCTGCTCA ACAATCTGCT 
GCAACTCTCA TTCTTCTAGA TGATCACAAC CACGAAGATC ACCTTCTTCT TCGCTTCCAA 
AGACGGGCGA GGTATAAAAT ATGGCTTTGG TGGAGTCGAT GCACCGTCGC CCTCACTATT 
ATTCAATTTG TGTGTGCTGC TTACCTTATC CTCAACTCTG TTTACTATTT ATCCAAAGAT 
AACACCACAC CAACTGCTTG TATTTTAGAA TTAGGATGGA ACCCTCACTG GTGGAACCAC 
AAGCTACTCT CTCTATTCAT CATCCTTCTC TCTTTCGTTG CTCTAGCTCA ATGCTTTACT 
GGATCCGATG TTCTCCGATG GAGATCTTTC TATGAATCCC ACGACAATGC TTGGAAATCC 
CATTACAGGG AAGTTTTTGA TAACGGCTTA CGCGAGACCT TGTGCTGTCT CGGCCGCGTC 
AAATACTTGA CTGCAATCCA ACAAGAAGAA GATGAAGTCT ATTCCGTTGC ACGCTTATTA 
GGCGATCTTG TTGCTTATCG TGCTTCTGGA ACTGGCCATA TGGAACTCTT GGCAGCTTTA 
GCTTTACTCC AAAGCAATGA AAAATCTTCA GAATCTTGTC AAGTATCCAT GGAAGCACCA 
GAGATGCGAA TCAGGGAGGC TGCAGCTCTT CATAAATTTG CTGAAGCTGC ATACACTGGT 
CCGTTGCTTG ATGTTGGACG AAATCCTCTC ATGTTTCCTT GTTCATGGCT CTATAGACAA 
GGGGTTCTGT CTCCTTGGGC ACGCAATAGA CGACCTGTTT TGGATGGTGA TAACTGGTGG 
CGAGGCCATG CTGCAGCCTT TCTAAAGTAT GCCAATTTGC CTCCGGAAGC GCTCAGACAT 
GGGAGAGTAA ACCAGGTCAA GTGTGAAGCT GCATATTTTA TTGTGGTTCT ACATCATCTA 
CAAACTGTAG TAATTGCTAT ACGTGGAACT GAAACCCCTG AGGACCTAAT AACCGATGGA 
TTATGCAAGG AATGCACCCT TTCTGCAGAA GACTTGGCTG GTTTGATAAA CTGCAACCAC 
ATTCATTCTG ATATACACAA AAATGTGGCT TCATCATTCC CCCACTATGG ACATTCGGGT 
ATAGTCGAGG CTGCACGAGA GCTTTATATG CAGATTGAAG GAAACCCTGG AGAGCATGAC 
ACTGAATCCT ATGGGCTTCT TTCTAAATTA CTGGGATTTG GATGTGAATG CTTTGGATAT 
AATGTCCGCA TCGTTGGACA TTCACTTGGG GGGGCTATAG CTGCATTGCT TGGACTGCAA 
CTTTATAACC GATACCCTAA TCTGCACGTT TACTCATATG GGCCACTCCC ATGTGTGGAT 
TTAGTTGTGG CTAATGCATG CTCATCATTT ATAACAAGCA TTATATATGG AAATGAGTTT 
TCATCCCGCC TTTCATTTGG ATCCATTATG AGGCTAAGGG CAGCTGCAAT CACATTACTG 
GCACAAGATC CTAAAGCTGA CAGTGCCATG ATTTTCAGAC TTGCTCGCCG GTTTCTATAT 
ATAAGCCAAT ATGAAAGAAA TAATCAAGAG GCAGAGCATG AAGCACAGTG TGACATTGAT 
AATAAAGGCA GCAACATACA AGTGGTTCAA GAAGCTTCAC TCTTGACTGA GGCAATTAAA 
AGGGATCTTC TTGTTACAGC TGACCATGGC AACAATGACC ATGATGACTT TTCACTCTGG 
GCTGATACCA GGGCAAGGGA CCATATTGTT GAAATTAATA ATGCCGAATT TACAAATCCT 
TTTGCTACAA ATGTACATTC AATAGATGAT CCGGTGTCTC AGTTTATAGA TTCTGTTCCA 
ACTTCTGAAA ATCAGTCTGC TGATGATCCC CCTGAGATGT ATCTGCCAGG ATTTGTAATT 
CATATTGTCC CAGACCAGAA AAGACCTCAA ACTGATTTTA AGATATCTTG GAGAACACAG 
GAGAGGGGGA AATGTTATAG AGCTTATGTA GCAAATAGAG AAAGCTTCAA AGATATTATT 
GTCTCACCAT CCATGTTCCT TGATCATCTC CCTTGGAGAT GTCATAATGC CTTGAAAAAA 
ATATTGAAAG ATCTAACTGC TAAAGATCAA GTAATGGAAC ACAACTTAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003592848.2
CDS	64..2397
Translation	MKWEEAEGREQRKMGVGNVRRMGIVLGICNALVFLLGSLLISHNYRLCDLRSLLPFVAVSFSAVVRILVMLQTASAQQSAATLILLDDHNHEDHLLLRFQRRARYKIWLWWSRCTVALTIIQFVCAAYLILNSVYYLSKDNTTPTACILELGWNPHWWNHKLLSLFIILLSFVALAQCFTGSDVLRWRSFYESHDNAWKSHYREVFDNGLRETLCCLGRVKYLTAIQQEEDEVYSVARLLGDLVAYRASGTGHMELLAALALLQSNEKSSESCQVSMEAPEMRIREAAALHKFAEAAYTGPLLDVGRNPLMFPCSWLYRQGVLSPWARNRRPVLDGDNWWRGHAAAFLKYANLPPEALRHGRVNQVKCEAAYFIVVLHHLQTVVIAIRGTETPEDLITDGLCKECTLSAEDLAGLINCNHIHSDIHKNVASSFPHYGHSGIVEAARELYMQIEGNPGEHDTESYGLLSKLLGFGCECFGYNVRIVGHSLGGAIAALLGLQLYNRYPNLHVYSYGPLPCVDLVVANACSSFITSIIYGNEFSSRLSFGSIMRLRAAAITLLAQDPKADSAMIFRLARRFLYISQYERNNQEAEHEAQCDIDNKGSNIQVVQEASLLTEAIKRDLLVTADHGNNDHDDFSLWADTRARDHIVEINNAEFTNPFATNVHSIDDPVSQFIDSVPTSENQSADDPPEMYLPGFVIHIVPDQKRPQTDFKISWRTQERGKCYRAYVANRESFKDIIVSPSMFLDHLPWRCHNALKKILKDLTAKDQVMEHNLI

Target ORF information:

RefSeq Version	XM_003592800.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula uncharacterized LOC11446024 (LOC11446024), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003592800.3

ORF Insert Sequence:

ATGAAATGGG AGGAGGCGGA AGGCAGGGAG CAGAGGAAAA TGGGTGTTGG AAACGTTAGA 
CGTATGGGGA TTGTTTTAGG GATTTGTAAC GCACTGGTTT TCCTCCTTGG TTCCCTTCTC 
ATTTCCCACA ATTATCGCCT CTGCGATCTC CGTTCTCTTC TCCCTTTCGT TGCCGTTTCA 
TTCTCTGCCG TTGTAAGGAT TCTTGTTATG CTTCAAACCG CTTCTGCTCA ACAATCTGCT 
GCAACTCTCA TTCTTCTAGA TGATCACAAC CACGAAGATC ACCTTCTTCT TCGCTTCCAA 
AGACGGGCGA GGTATAAAAT ATGGCTTTGG TGGAGTCGAT GCACCGTCGC CCTCACTATT 
ATTCAATTTG TGTGTGCTGC TTACCTTATC CTCAACTCTG TTTACTATTT ATCCAAAGAT 
AACACCACAC CAACTGCTTG TATTTTAGAA TTAGGATGGA ACCCTCACTG GTGGAACCAC 
AAGCTACTCT CTCTATTCAT CATCCTTCTC TCTTTCGTTG CTCTAGCTCA ATGCTTTACT 
GGATCCGATG TTCTCCGATG GAGATCTTTC TATGAATCCC ACGACAATGC TTGGAAATCC 
CATTACAGGG AAGTTTTTGA TAACGGCTTA CGCGAGACCT TGTGCTGTCT CGGCCGCGTC 
AAATACTTGA CTGCAATCCA ACAAGAAGAA GATGAAGTCT ATTCCGTTGC ACGCTTATTA 
GGCGATCTTG TTGCTTATCG TGCTTCTGGA ACTGGCCATA TGGAACTCTT GGCAGCTTTA 
GCTTTACTCC AAAGCAATGA AAAATCTTCA GAATCTTGTC AAGTATCCAT GGAAGCACCA 
GAGATGCGAA TCAGGGAGGC TGCAGCTCTT CATAAATTTG CTGAAGCTGC ATACACTGGT 
CCGTTGCTTG ATGTTGGACG AAATCCTCTC ATGTTTCCTT GTTCATGGCT CTATAGACAA 
GGGGTTCTGT CTCCTTGGGC ACGCAATAGA CGACCTGTTT TGGATGGTGA TAACTGGTGG 
CGAGGCCATG CTGCAGCCTT TCTAAAGTAT GCCAATTTGC CTCCGGAAGC GCTCAGACAT 
GGGAGAGTAA ACCAGGTCAA GTGTGAAGCT GCATATTTTA TTGTGGTTCT ACATCATCTA 
CAAACTGTAG TAATTGCTAT ACGTGGAACT GAAACCCCTG AGGACCTAAT AACCGATGGA 
TTATGCAAGG AATGCACCCT TTCTGCAGAA GACTTGGCTG GTTTGATAAA CTGCAACCAC 
ATTCATTCTG ATATACACAA AAATGTGGCT TCATCATTCC CCCACTATGG ACATTCGGGT 
ATAGTCGAGG CTGCACGAGA GCTTTATATG CAGATTGAAG GAAACCCTGG AGAGCATGAC 
ACTGAATCCT ATGGGCTTCT TTCTAAATTA CTGGGATTTG GATGTGAATG CTTTGGATAT 
AATGTCCGCA TCGTTGGACA TTCACTTGGG GGGGCTATAG CTGCATTGCT TGGACTGCAA 
CTTTATAACC GATACCCTAA TCTGCACGTT TACTCATATG GGCCACTCCC ATGTGTGGAT 
TTAGTTGTGG CTAATGCATG CTCATCATTT ATAACAAGCA TTATATATGG AAATGAGTTT 
TCATCCCGCC TTTCATTTGG ATCCATTATG AGGCTAAGGG CAGCTGCAAT CACATTACTG 
GCACAAGATC CTAAAGCTGA CAGTGCCATG ATTTTCAGAC TTGCTCGCCG GTTTCTATAT 
ATAAGCCAAT ATGAAAGAAA TAATCAAGAG GCAGAGCATG AAGCACAGTG TGACATTGAT 
AATAAAGGCA GCAACATACA AGTGGTTCAA GAAGCTTCAC TCTTGACTGA GGCAATTAAA 
AGGGATCTTC TTGTTACAGC TGACCATGGC AACAATGACC ATGATGACTT TTCACTCTGG 
GCTGATACCA GGGCAAGGGA CCATATTGTT GAAATTAATA ATGCCGAATT TACAAATCCT 
TTTGCTACAA ATGTACATTC AATAGATGAT CCGGTGTCTC AGTTTATAGA TTCTGTTCCA 
ACTTCTGAAA ATCAGTCTGC TGATGATCCC CCTGAGATGT ATCTGCCAGG ATTTGTAATT 
CATATTGTCC CAGACCAGAA AAGACCTCAA ACTGATTTTA AGATATCTTG GAGAACACAG 
GAGAGGGGGA AATGTTATAG AGCTTATGTA GCAAATAGAG AAAGCTTCAA AGATATTATT 
GTCTCACCAT CCATGTTCCT TGATCATCTC CCTTGGAGAT GTCATAATGC CTTGAAAAAA 
ATATTGAAAG ATCTAACTGC TAAAGATCAA GTAATGGAAC ACAACTTAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11446024 ( XM_013615057.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013470511.1 Medicago truncatula sn1-specific diacylglycerol lipase beta-like protein mRNA.

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CloneID	OMe213876
Clone ID Related Accession (Same CDS sequence)	XM_013615057.1
Accession Version	XM_013615057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	sn1-specific diacylglycerol lipase beta-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGAAATGGG AGGAGGCGGA AGGCAGGGAG CAGAGGAAAA TGGGTGTTGG AAACGTTAGA 
CGTATGGGGA TTGTTTTAGG GATTTGTAAC GCACTGGTTT TCCTCCTTGG TTCCCTTCTC 
ATTTCCCACA ATTATCGCCT CTGCGATCTC CGTTCTCTTC TCCCTTTCGT TGCCGTTTCA 
TTCTCTGCCG TTGTAAGGAT TCTTGTTATG CTTCAAACCG CTTCTGCTCA ACAATCTGCT 
GCAACTCTCA TTCTTCTAGA TGATCACAAC CACGAAGATC ACCTTCTTCT TCGCTTCCAA 
AGACGGGCGA GGTATAAAAT ATGGCTTTGG TGGAGTCGAT GCACCGTCGC CCTCACTATT 
ATTCAATTTG TGTGTGCTGC TTACCTTATC CTCAACTCTG TTTACTATTT ATCCAAAGAT 
AACACCACAC CAACTGCTTG TATTTTAGAA TTAGGATGGA ACCCTCACTG GTGGAACCAC 
AAGCTACTCT CTCTATTCAT CATCCTTCTC TCTTTCGTTG CTCTAGCTCA ATGCTTTACT 
GGATCCGATG TTCTCCGATG GAGATCTTTC TATGAATCCC ACGACAATGC TTGGAAATCC 
CATTACAGGG AAGTTTTTGA TAACGGCTTA CGCGAGACCT TGTGCTGTCT CGGCCGCGTC 
AAATACTTGA CTGCAATCCA ACAAGAAGAA GATGAAGTCT ATTCCGTTGC ACGCTTATTA 
GGCGATCTTG TTGCTTATCG TGCTTCTGGA ACTGGCCATA TGGAACTCTT GGCAGCTTTA 
GCTTTACTCC AAAGCAATGA AAAATCTTCA GAATCTTGTC AAGTATCCAT GGAAGCACCA 
GAGATGCGAA TCAGGGAGGC TGCAGCTCTT CATAAATTTG CTGAAGCTGC ATACACTGGT 
CCGTTGCTTG ATGTTGGACG AAATCCTCTC ATGTTTCCTT GTTCATGGCT CTATAGACAA 
GGGGTTCTGT CTCCTTGGGC ACGCAATAGA CGACCTGTTT TGGATGGTGA TAACTGGTGG 
CGAGGCCATG CTGCAGCCTT TCTAAAGTAT GCCAATTTGC CTCCGGAAGC GCTCAGACAT 
GGGAGAGTAA ACCAGGTCAA GTGTGAAGCT GCATATTTTA TTGTGGTTCT ACATCATCTA 
CAAACTGTAG TAATTGCTAT ACGTGGAACT GAAACCCCTG AGGACCTAAT AACCGATGGA 
TTATGCAAGG AATGCACCCT TTCTGCAGAA GACTTGGCTG GTTTGATAAA CTGCAACCAC 
ATTCATTCTG ATATACACAA AAATGTGGCT TCATCATTCC CCCACTATGG ACATTCGGGT 
ATAGTCGAGG CTGCACGAGA GCTTTATATG CAGATTGAAG GAAACCCTGG AGAGCATGAC 
ACTGAATCCT ATGGGCTTCT TTCTAAATTA CTGGGATTTG GATGTGAATG CTTTGGATAT 
AATGTCCGCA TCGTTGGACA TTCACTTGGG GGGGCTATAG CTGCATTGCT TGGACTGCAA 
CTTTATAACC GATACCCTAA TCTGCACGTT TACTCATATG GGCCACTCCC ATGTGTGGAT 
TTAGTTGTGG CTAATGCATG CTCATCATTT ATAACAAGCA TTATATATGG AAATGAGTTT 
TCATCCCGCC TTTCATTTGG ATCCATTATG AGGCTAAGGG CAGCTGCAAT CACATTACTG 
GCACAAGATC CTAAAGCTGA CAGTGCCATG ATTTTCAGAC TTGCTCGCCG GTTTCTATAT 
ATAAGCCAAT ATGAAAGAAA TAATCAAGAG GCAGAGCATG AAGCACAGTG TGACATTGAT 
AATAAAGATT GGCGAGAGGT TCCTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013470511.1
CDS	91..1920
Translation	MKWEEAEGREQRKMGVGNVRRMGIVLGICNALVFLLGSLLISHNYRLCDLRSLLPFVAVSFSAVVRILVMLQTASAQQSAATLILLDDHNHEDHLLLRFQRRARYKIWLWWSRCTVALTIIQFVCAAYLILNSVYYLSKDNTTPTACILELGWNPHWWNHKLLSLFIILLSFVALAQCFTGSDVLRWRSFYESHDNAWKSHYREVFDNGLRETLCCLGRVKYLTAIQQEEDEVYSVARLLGDLVAYRASGTGHMELLAALALLQSNEKSSESCQVSMEAPEMRIREAAALHKFAEAAYTGPLLDVGRNPLMFPCSWLYRQGVLSPWARNRRPVLDGDNWWRGHAAAFLKYANLPPEALRHGRVNQVKCEAAYFIVVLHHLQTVVIAIRGTETPEDLITDGLCKECTLSAEDLAGLINCNHIHSDIHKNVASSFPHYGHSGIVEAARELYMQIEGNPGEHDTESYGLLSKLLGFGCECFGYNVRIVGHSLGGAIAALLGLQLYNRYPNLHVYSYGPLPCVDLVVANACSSFITSIIYGNEFSSRLSFGSIMRLRAAAITLLAQDPKADSAMIFRLARRFLYISQYERNNQEAEHEAQCDIDNKDWREVPF

Target ORF information:

RefSeq Version	XM_013615057.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula sn1-specific diacylglycerol lipase beta-like protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013615057.1

ORF Insert Sequence:

ATGAAATGGG AGGAGGCGGA AGGCAGGGAG CAGAGGAAAA TGGGTGTTGG AAACGTTAGA 
CGTATGGGGA TTGTTTTAGG GATTTGTAAC GCACTGGTTT TCCTCCTTGG TTCCCTTCTC 
ATTTCCCACA ATTATCGCCT CTGCGATCTC CGTTCTCTTC TCCCTTTCGT TGCCGTTTCA 
TTCTCTGCCG TTGTAAGGAT TCTTGTTATG CTTCAAACCG CTTCTGCTCA ACAATCTGCT 
GCAACTCTCA TTCTTCTAGA TGATCACAAC CACGAAGATC ACCTTCTTCT TCGCTTCCAA 
AGACGGGCGA GGTATAAAAT ATGGCTTTGG TGGAGTCGAT GCACCGTCGC CCTCACTATT 
ATTCAATTTG TGTGTGCTGC TTACCTTATC CTCAACTCTG TTTACTATTT ATCCAAAGAT 
AACACCACAC CAACTGCTTG TATTTTAGAA TTAGGATGGA ACCCTCACTG GTGGAACCAC 
AAGCTACTCT CTCTATTCAT CATCCTTCTC TCTTTCGTTG CTCTAGCTCA ATGCTTTACT 
GGATCCGATG TTCTCCGATG GAGATCTTTC TATGAATCCC ACGACAATGC TTGGAAATCC 
CATTACAGGG AAGTTTTTGA TAACGGCTTA CGCGAGACCT TGTGCTGTCT CGGCCGCGTC 
AAATACTTGA CTGCAATCCA ACAAGAAGAA GATGAAGTCT ATTCCGTTGC ACGCTTATTA 
GGCGATCTTG TTGCTTATCG TGCTTCTGGA ACTGGCCATA TGGAACTCTT GGCAGCTTTA 
GCTTTACTCC AAAGCAATGA AAAATCTTCA GAATCTTGTC AAGTATCCAT GGAAGCACCA 
GAGATGCGAA TCAGGGAGGC TGCAGCTCTT CATAAATTTG CTGAAGCTGC ATACACTGGT 
CCGTTGCTTG ATGTTGGACG AAATCCTCTC ATGTTTCCTT GTTCATGGCT CTATAGACAA 
GGGGTTCTGT CTCCTTGGGC ACGCAATAGA CGACCTGTTT TGGATGGTGA TAACTGGTGG 
CGAGGCCATG CTGCAGCCTT TCTAAAGTAT GCCAATTTGC CTCCGGAAGC GCTCAGACAT 
GGGAGAGTAA ACCAGGTCAA GTGTGAAGCT GCATATTTTA TTGTGGTTCT ACATCATCTA 
CAAACTGTAG TAATTGCTAT ACGTGGAACT GAAACCCCTG AGGACCTAAT AACCGATGGA 
TTATGCAAGG AATGCACCCT TTCTGCAGAA GACTTGGCTG GTTTGATAAA CTGCAACCAC 
ATTCATTCTG ATATACACAA AAATGTGGCT TCATCATTCC CCCACTATGG ACATTCGGGT 
ATAGTCGAGG CTGCACGAGA GCTTTATATG CAGATTGAAG GAAACCCTGG AGAGCATGAC 
ACTGAATCCT ATGGGCTTCT TTCTAAATTA CTGGGATTTG GATGTGAATG CTTTGGATAT 
AATGTCCGCA TCGTTGGACA TTCACTTGGG GGGGCTATAG CTGCATTGCT TGGACTGCAA 
CTTTATAACC GATACCCTAA TCTGCACGTT TACTCATATG GGCCACTCCC ATGTGTGGAT 
TTAGTTGTGG CTAATGCATG CTCATCATTT ATAACAAGCA TTATATATGG AAATGAGTTT 
TCATCCCGCC TTTCATTTGG ATCCATTATG AGGCTAAGGG CAGCTGCAAT CACATTACTG 
GCACAAGATC CTAAAGCTGA CAGTGCCATG ATTTTCAGAC TTGCTCGCCG GTTTCTATAT 
ATAAGCCAAT ATGAAAGAAA TAATCAAGAG GCAGAGCATG AAGCACAGTG TGACATTGAT 
AATAAAGATT GGCGAGAGGT TCCTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11446024 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

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