LOC11439065 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11439065
Entrez Gene ID	11439065
Full Name	actin-binding FH2 (formin 2) family protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024782692.1	XP_024638460.1	formin-like protein 13 isoform X3
XM_024782693.1	XP_024638461.1	formin-like protein 13 isoform X4
XM_013603141.1	XP_013458595.1	actin-binding FH2 (formin 2) family protein
XM_024782690.1	XP_024638458.1	formin-like protein 13 isoform X1
XM_003610287.2	XP_003610335.1	actin-binding FH2 (formin 2) family protein
XM_024782691.1	XP_024638459.1	formin-like protein 13 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11439065 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11439065 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe190720	XM_013603141.1 Latest version!	Medicago truncatula actin-binding FH2 (formin 2) family protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OMe193025	XM_003610287.2 Latest version!	Medicago truncatula actin-binding FH2 (formin 2) family protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe193025	XM_024782690.1 Latest version!	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe264251	XM_024782691.1 Latest version!	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMe264252	XM_024782692.1 Latest version!	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMe264253	XM_024782693.1 Latest version!	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11439065 ( XM_013603141.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013458595.1 Medicago truncatula actin-binding FH2 (formin 2) family protein partial mRNA.

Price & Availability↑

CloneID	OMe190720
Clone ID Related Accession (Same CDS sequence)	XM_013603141.1
Accession Version	XM_013603141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	actin-binding FH2 (formin 2) family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016410). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGGTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013458595.1
CDS	1..3441
Translation	MAMLRKLFYRKPPDGLLEICDRVYVFDCCFTTEAWNEEKYKVYMDGIVGQLRENVPDASILVFNFREEETKSLMANIISEYDITIMDYPRHYEGCPVLKMELIHHFLRSSESWLSLGQHNVLLMHCERGGWPVMAFMLAALLIYRKVYSGEHRTLDMIYRQSPHQLLHLLTPLNPIPSQLRYLQYVSRRNVALDWPPLDRALMLDCIILRFFPNFDGEGGCHPLFRIYGQDPFSADKSPKMLYSMPKRSKNVRAYKQGECELIKIDINCHIQGDVVIEGINLNDDMEHEMMMFRVMFNTAFVRSNILMLNRDEIDVLWDAKDHFPKDFRAEILFSEIDAAAAVILDNTSFFEEKDGLPIEAFAKVQEIFSHVDWMNPKADAALNVLQQISTSAIMNDKLDKVSDQHVETATSLGETGPKTPQRNSYAAIRSLSSTKRTPNNDMSRKEEKTNKVDSIPQRANTSDIIGQEKICSSEKSLESSKCPTGSTNFDIKPQESNLASSSSADSSLSPGTPPPRPPLTTRSKEVHDSPPHTELPPHHILPSQSGPQSQDRSYSPISSSTPETYHSLAPDSSIEPSPPLSSRKPLNDIPPVKTRPKSPSSQPPTPPPPPTPPLKDQKVVRAGPLSPPPPPPSPKKDIHVKAGPPPSPPVPSHMNEKPHVRDGLSPPPPPLPPSPPLKAEQPTRFQPPPPPPPPRLSVEAASPITAPPAPPPPPSATLSSGNPNASLQKSSPAPPPPIPFGKGLKPGSAFPMSLSVGVDGNKVSGPQSSSLAGSKGRVLPRAIGSKNDSKKLKPLHWMKLSRAVQGSLWDETQKSGEASKAPEIDMSELESLFSAAAPSSGPAKKSNVQSSVKPKSEKVQLIDHRRAYNCEIMLSKVKVPLHDLMSSVLALEESALDTDTVENLIKFCPTKEEMEIIKNYNGEKEKLGRCEQFFMELMKVPRVEAKLRVFSFRIQFYSQVSDLKNSLKVVNSSAEEIRNSVKLKRIMQTILTLGNALNQGTARGSAIGFRLDSLLKLTETRARNNKMTLMHYLCKVLDDKLPEVLDFSKDLANLEPAAKIQLKFLAEEMQAVNKGLEKVVQELSTSENDGPISETFRKKLKGFLCSAEAEVRTLASLYSGVGKNVDALILYFGEDPSRCQFEQGV

Target ORF information:

RefSeq Version	XM_013603141.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula actin-binding FH2 (formin 2) family protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013603141.1

ORF Insert Sequence:

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGGTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11439065 ( XM_024782690.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024638458.1 Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe193025
Clone ID Related Accession (Same CDS sequence)	XM_024782690.1 , XM_003610287.2
Accession Version	XM_024782690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	formin-like protein 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGTTGTCAC AACTTTGCTC AACTTTACTC GAATGTTCAA CAAAGCCCAT 
GAAGAAAATC GTAAGCAGCT AGAGCTTGAA ATGAAGAAAA CAGCAGAAAG TGATAAAAAG 
AAATGTGAAT CGGAAAGGAT ATTACCTACA ACGATCCGAA CTGGCAATGT CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024638458.1
CDS	227..3823
Translation	MAMLRKLFYRKPPDGLLEICDRVYVFDCCFTTEAWNEEKYKVYMDGIVGQLRENVPDASILVFNFREEETKSLMANIISEYDITIMDYPRHYEGCPVLKMELIHHFLRSSESWLSLGQHNVLLMHCERGGWPVMAFMLAALLIYRKVYSGEHRTLDMIYRQSPHQLLHLLTPLNPIPSQLRYLQYVSRRNVALDWPPLDRALMLDCIILRFFPNFDGEGGCHPLFRIYGQDPFSADKSPKMLYSMPKRSKNVRAYKQGECELIKIDINCHIQGDVVIEGINLNDDMEHEMMMFRVMFNTAFVRSNILMLNRDEIDVLWDAKDHFPKDFRAEILFSEIDAAAAVILDNTSFFEEKDGLPIEAFAKVQEIFSHVDWMNPKADAALNVLQQISTSAIMNDKLDKVSDQHVETATSLGETGPKTPQRNSYAAIRSLSSTKRTPNNDMSRKEEKTNKVDSIPQRANTSDIIGQEKICSSEKSLESSKCPTGSTNFDIKPQESNLASSSSADSSLSPGTPPPRPPLTTRSKEVHDSPPHTELPPHHILPSQSGPQSQDRSYSPISSSTPETYHSLAPDSSIEPSPPLSSRKPLNDIPPVKTRPKSPSSQPPTPPPPPTPPLKDQKVVRAGPLSPPPPPPSPKKDIHVKAGPPPSPPVPSHMNEKPHVRDGLSPPPPPLPPSPPLKAEQPTRFQPPPPPPPPRLSVEAASPITAPPAPPPPPSATLSSGNPNASLQKSSPAPPPPIPFGKGLKPGSAFPMSLSVGVDGNKVSGPQSSSLAGSKGRVLPRAIGSKNDSKKLKPLHWMKLSRAVQGSLWDETQKSGEASKAPEIDMSELESLFSAAAPSSGPAKKSNVQSSVKPKSEKVQLIDHRRAYNCEIMLSKVKVPLHDLMSSVLALEESALDTDTVENLIKFCPTKEEMEIIKNYNGEKEKLGRCEQFFMELMKVPRVEAKLRVFSFRIQFYSQVSDLKNSLKVVNSSAEEIRNSVKLKRIMQTILTLGNALNQGTARGSAIGFRLDSLLKLTETRARNNKMTLMHYLCKVLDDKLPEVLDFSKDLANLEPAAKIQLKFLAEEMQAVNKGLEKVVQELSTSENDGPISETFRKKLKGFLCSAEAEVRTLASLYSGVGKNVDALILYFGEDPSRCQFEQVVTTLLNFTRMFNKAHEENRKQLELEMKKTAESDKKKCESERILPTTIRTGNVK

Target ORF information:

RefSeq Version	XM_024782690.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024782690.1

ORF Insert Sequence:

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGTTGTCAC AACTTTGCTC AACTTTACTC GAATGTTCAA CAAAGCCCAT 
GAAGAAAATC GTAAGCAGCT AGAGCTTGAA ATGAAGAAAA CAGCAGAAAG TGATAAAAAG 
AAATGTGAAT CGGAAAGGAT ATTACCTACA ACGATCCGAA CTGGCAATGT CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11439065 ( XM_003610287.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003610335.1 Medicago truncatula actin-binding FH2 (formin 2) family protein partial mRNA.

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CloneID	OMe193025
Clone ID Related Accession (Same CDS sequence)	XM_024782690.1 , XM_003610287.2
Accession Version	XM_003610287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	actin-binding FH2 (formin 2) family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016410). On Aug 25, 2015 this sequence version replaced XM_003610287.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGTTGTCAC AACTTTGCTC AACTTTACTC GAATGTTCAA CAAAGCCCAT 
GAAGAAAATC GTAAGCAGCT AGAGCTTGAA ATGAAGAAAA CAGCAGAAAG TGATAAAAAG 
AAATGTGAAT CGGAAAGGAT ATTACCTACA ACGATCCGAA CTGGCAATGT CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003610335.1
CDS	1..3597
Translation	MAMLRKLFYRKPPDGLLEICDRVYVFDCCFTTEAWNEEKYKVYMDGIVGQLRENVPDASILVFNFREEETKSLMANIISEYDITIMDYPRHYEGCPVLKMELIHHFLRSSESWLSLGQHNVLLMHCERGGWPVMAFMLAALLIYRKVYSGEHRTLDMIYRQSPHQLLHLLTPLNPIPSQLRYLQYVSRRNVALDWPPLDRALMLDCIILRFFPNFDGEGGCHPLFRIYGQDPFSADKSPKMLYSMPKRSKNVRAYKQGECELIKIDINCHIQGDVVIEGINLNDDMEHEMMMFRVMFNTAFVRSNILMLNRDEIDVLWDAKDHFPKDFRAEILFSEIDAAAAVILDNTSFFEEKDGLPIEAFAKVQEIFSHVDWMNPKADAALNVLQQISTSAIMNDKLDKVSDQHVETATSLGETGPKTPQRNSYAAIRSLSSTKRTPNNDMSRKEEKTNKVDSIPQRANTSDIIGQEKICSSEKSLESSKCPTGSTNFDIKPQESNLASSSSADSSLSPGTPPPRPPLTTRSKEVHDSPPHTELPPHHILPSQSGPQSQDRSYSPISSSTPETYHSLAPDSSIEPSPPLSSRKPLNDIPPVKTRPKSPSSQPPTPPPPPTPPLKDQKVVRAGPLSPPPPPPSPKKDIHVKAGPPPSPPVPSHMNEKPHVRDGLSPPPPPLPPSPPLKAEQPTRFQPPPPPPPPRLSVEAASPITAPPAPPPPPSATLSSGNPNASLQKSSPAPPPPIPFGKGLKPGSAFPMSLSVGVDGNKVSGPQSSSLAGSKGRVLPRAIGSKNDSKKLKPLHWMKLSRAVQGSLWDETQKSGEASKAPEIDMSELESLFSAAAPSSGPAKKSNVQSSVKPKSEKVQLIDHRRAYNCEIMLSKVKVPLHDLMSSVLALEESALDTDTVENLIKFCPTKEEMEIIKNYNGEKEKLGRCEQFFMELMKVPRVEAKLRVFSFRIQFYSQVSDLKNSLKVVNSSAEEIRNSVKLKRIMQTILTLGNALNQGTARGSAIGFRLDSLLKLTETRARNNKMTLMHYLCKVLDDKLPEVLDFSKDLANLEPAAKIQLKFLAEEMQAVNKGLEKVVQELSTSENDGPISETFRKKLKGFLCSAEAEVRTLASLYSGVGKNVDALILYFGEDPSRCQFEQVVTTLLNFTRMFNKAHEENRKQLELEMKKTAESDKKKCESERILPTTIRTGNVK

Target ORF information:

RefSeq Version	XM_003610287.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula actin-binding FH2 (formin 2) family protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003610287.2

ORF Insert Sequence:

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGTTGTCAC AACTTTGCTC AACTTTACTC GAATGTTCAA CAAAGCCCAT 
GAAGAAAATC GTAAGCAGCT AGAGCTTGAA ATGAAGAAAA CAGCAGAAAG TGATAAAAAG 
AAATGTGAAT CGGAAAGGAT ATTACCTACA ACGATCCGAA CTGGCAATGT CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11439065 ( XM_024782691.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024638459.1 Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe264251
Clone ID Related Accession (Same CDS sequence)	XM_024782691.1
Accession Version	XM_024782691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	formin-like protein 13 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGCAAGTTG GTGCATGAAT TCATCACTTG TGCTTGGCTG CATATATATG 
AATTTTCAAA ATTTCAATGT CAAAATTTGT CTATCTGTAG CTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024638459.1
CDS	227..3754
Translation	MAMLRKLFYRKPPDGLLEICDRVYVFDCCFTTEAWNEEKYKVYMDGIVGQLRENVPDASILVFNFREEETKSLMANIISEYDITIMDYPRHYEGCPVLKMELIHHFLRSSESWLSLGQHNVLLMHCERGGWPVMAFMLAALLIYRKVYSGEHRTLDMIYRQSPHQLLHLLTPLNPIPSQLRYLQYVSRRNVALDWPPLDRALMLDCIILRFFPNFDGEGGCHPLFRIYGQDPFSADKSPKMLYSMPKRSKNVRAYKQGECELIKIDINCHIQGDVVIEGINLNDDMEHEMMMFRVMFNTAFVRSNILMLNRDEIDVLWDAKDHFPKDFRAEILFSEIDAAAAVILDNTSFFEEKDGLPIEAFAKVQEIFSHVDWMNPKADAALNVLQQISTSAIMNDKLDKVSDQHVETATSLGETGPKTPQRNSYAAIRSLSSTKRTPNNDMSRKEEKTNKVDSIPQRANTSDIIGQEKICSSEKSLESSKCPTGSTNFDIKPQESNLASSSSADSSLSPGTPPPRPPLTTRSKEVHDSPPHTELPPHHILPSQSGPQSQDRSYSPISSSTPETYHSLAPDSSIEPSPPLSSRKPLNDIPPVKTRPKSPSSQPPTPPPPPTPPLKDQKVVRAGPLSPPPPPPSPKKDIHVKAGPPPSPPVPSHMNEKPHVRDGLSPPPPPLPPSPPLKAEQPTRFQPPPPPPPPRLSVEAASPITAPPAPPPPPSATLSSGNPNASLQKSSPAPPPPIPFGKGLKPGSAFPMSLSVGVDGNKVSGPQSSSLAGSKGRVLPRAIGSKNDSKKLKPLHWMKLSRAVQGSLWDETQKSGEASKAPEIDMSELESLFSAAAPSSGPAKKSNVQSSVKPKSEKVQLIDHRRAYNCEIMLSKVKVPLHDLMSSVLALEESALDTDTVENLIKFCPTKEEMEIIKNYNGEKEKLGRCEQFFMELMKVPRVEAKLRVFSFRIQFYSQVSDLKNSLKVVNSSAEEIRNSVKLKRIMQTILTLGNALNQGTARGSAIGFRLDSLLKLTETRARNNKMTLMHYLCKVLDDKLPEVLDFSKDLANLEPAAKIQLKFLAEEMQAVNKGLEKVVQELSTSENDGPISETFRKKLKGFLCSAEAEVRTLASLYSGVGKNVDALILYFGEDPSRCQFEQASWCMNSSLVLGCIYMNFQNFNVKICLSVAS

Target ORF information:

RefSeq Version	XM_024782691.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024782691.1

ORF Insert Sequence:

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGAAA 
TTGAAAGGGT TCCTCTGTTC TGCTGAAGCT GAAGTCCGAA CATTGGCTTC ACTATATTCT 
GGTGTGGGCA AGAACGTGGA TGCGTTGATT CTTTATTTTG GGGAAGATCC ATCTCGCTGT 
CAATTTGAGC AAGCAAGTTG GTGCATGAAT TCATCACTTG TGCTTGGCTG CATATATATG 
AATTTTCAAA ATTTCAATGT CAAAATTTGT CTATCTGTAG CTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11439065 ( XM_024782692.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024638460.1 Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe264252
Clone ID Related Accession (Same CDS sequence)	XM_024782692.1
Accession Version	XM_024782692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	formin-like protein 13 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGGGC 
AAGAACGTGG ATGCGTTGAT TCTTTATTTT GGGGAAGATC CATCTCGCTG TCAATTTGAG 
CAAGTTGTCA CAACTTTGCT CAACTTTACT CGAATGTTCA ACAAAGCCCA TGAAGAAAAT 
CGTAAGCAGC TAGAGCTTGA AATGAAGAAA ACAGCAGAAA GTGATAAAAA GAAATGTGAA 
TCGGAAAGGA TATTACCTAC AACGATCCGA ACTGGCAATG TCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024638460.1
CDS	227..3754
Translation	MAMLRKLFYRKPPDGLLEICDRVYVFDCCFTTEAWNEEKYKVYMDGIVGQLRENVPDASILVFNFREEETKSLMANIISEYDITIMDYPRHYEGCPVLKMELIHHFLRSSESWLSLGQHNVLLMHCERGGWPVMAFMLAALLIYRKVYSGEHRTLDMIYRQSPHQLLHLLTPLNPIPSQLRYLQYVSRRNVALDWPPLDRALMLDCIILRFFPNFDGEGGCHPLFRIYGQDPFSADKSPKMLYSMPKRSKNVRAYKQGECELIKIDINCHIQGDVVIEGINLNDDMEHEMMMFRVMFNTAFVRSNILMLNRDEIDVLWDAKDHFPKDFRAEILFSEIDAAAAVILDNTSFFEEKDGLPIEAFAKVQEIFSHVDWMNPKADAALNVLQQISTSAIMNDKLDKVSDQHVETATSLGETGPKTPQRNSYAAIRSLSSTKRTPNNDMSRKEEKTNKVDSIPQRANTSDIIGQEKICSSEKSLESSKCPTGSTNFDIKPQESNLASSSSADSSLSPGTPPPRPPLTTRSKEVHDSPPHTELPPHHILPSQSGPQSQDRSYSPISSSTPETYHSLAPDSSIEPSPPLSSRKPLNDIPPVKTRPKSPSSQPPTPPPPPTPPLKDQKVVRAGPLSPPPPPPSPKKDIHVKAGPPPSPPVPSHMNEKPHVRDGLSPPPPPLPPSPPLKAEQPTRFQPPPPPPPPRLSVEAASPITAPPAPPPPPSATLSSGNPNASLQKSSPAPPPPIPFGKGLKPGSAFPMSLSVGVDGNKVSGPQSSSLAGSKGRVLPRAIGSKNDSKKLKPLHWMKLSRAVQGSLWDETQKSGEASKAPEIDMSELESLFSAAAPSSGPAKKSNVQSSVKPKSEKVQLIDHRRAYNCEIMLSKVKVPLHDLMSSVLALEESALDTDTVENLIKFCPTKEEMEIIKNYNGEKEKLGRCEQFFMELMKVPRVEAKLRVFSFRIQFYSQVSDLKNSLKVVNSSAEEIRNSVKLKRIMQTILTLGNALNQGTARGSAIGFRLDSLLKLTETRARNNKMTLMHYLCKVLDDKLPEVLDFSKDLANLEPAAKIQLKFLAEEMQAVNKGLEKVVQELSTSENDGPISETFRKGKNVDALILYFGEDPSRCQFEQVVTTLLNFTRMFNKAHEENRKQLELEMKKTAESDKKKCESERILPTTIRTGNVK

Target ORF information:

RefSeq Version	XM_024782692.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024782692.1

ORF Insert Sequence:

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGGGC 
AAGAACGTGG ATGCGTTGAT TCTTTATTTT GGGGAAGATC CATCTCGCTG TCAATTTGAG 
CAAGTTGTCA CAACTTTGCT CAACTTTACT CGAATGTTCA ACAAAGCCCA TGAAGAAAAT 
CGTAAGCAGC TAGAGCTTGA AATGAAGAAA ACAGCAGAAA GTGATAAAAA GAAATGTGAA 
TCGGAAAGGA TATTACCTAC AACGATCCGA ACTGGCAATG TCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11439065 ( XM_024782693.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024638461.1 Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X4, mRNA.

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CloneID	OMe264253
Clone ID Related Accession (Same CDS sequence)	XM_024782693.1
Accession Version	XM_024782693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	formin-like protein 13 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGGGC 
AAGAACGTGG ATGCGTTGAT TCTTTATTTT GGGGAAGATC CATCTCGCTG TCAATTTGAG 
CAAGCAAGTT GGTGCATGAA TTCATCACTT GTGCTTGGCT GCATATATAT GAATTTTCAA 
AATTTCAATG TCAAAATTTG TCTATCTGTA GCTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024638461.1
CDS	228..3686
Translation	MAMLRKLFYRKPPDGLLEICDRVYVFDCCFTTEAWNEEKYKVYMDGIVGQLRENVPDASILVFNFREEETKSLMANIISEYDITIMDYPRHYEGCPVLKMELIHHFLRSSESWLSLGQHNVLLMHCERGGWPVMAFMLAALLIYRKVYSGEHRTLDMIYRQSPHQLLHLLTPLNPIPSQLRYLQYVSRRNVALDWPPLDRALMLDCIILRFFPNFDGEGGCHPLFRIYGQDPFSADKSPKMLYSMPKRSKNVRAYKQGECELIKIDINCHIQGDVVIEGINLNDDMEHEMMMFRVMFNTAFVRSNILMLNRDEIDVLWDAKDHFPKDFRAEILFSEIDAAAAVILDNTSFFEEKDGLPIEAFAKVQEIFSHVDWMNPKADAALNVLQQISTSAIMNDKLDKVSDQHVETATSLGETGPKTPQRNSYAAIRSLSSTKRTPNNDMSRKEEKTNKVDSIPQRANTSDIIGQEKICSSEKSLESSKCPTGSTNFDIKPQESNLASSSSADSSLSPGTPPPRPPLTTRSKEVHDSPPHTELPPHHILPSQSGPQSQDRSYSPISSSTPETYHSLAPDSSIEPSPPLSSRKPLNDIPPVKTRPKSPSSQPPTPPPPPTPPLKDQKVVRAGPLSPPPPPPSPKKDIHVKAGPPPSPPVPSHMNEKPHVRDGLSPPPPPLPPSPPLKAEQPTRFQPPPPPPPPRLSVEAASPITAPPAPPPPPSATLSSGNPNASLQKSSPAPPPPIPFGKGLKPGSAFPMSLSVGVDGNKVSGPQSSSLAGSKGRVLPRAIGSKNDSKKLKPLHWMKLSRAVQGSLWDETQKSGEASKAPEIDMSELESLFSAAAPSSGPAKKSNVQSSVKPKSEKVQLIDHRRAYNCEIMLSKVKVPLHDLMSSVLALEESALDTDTVENLIKFCPTKEEMEIIKNYNGEKEKLGRCEQFFMELMKVPRVEAKLRVFSFRIQFYSQVSDLKNSLKVVNSSAEEIRNSVKLKRIMQTILTLGNALNQGTARGSAIGFRLDSLLKLTETRARNNKMTLMHYLCKVLDDKLPEVLDFSKDLANLEPAAKIQLKFLAEEMQAVNKGLEKVVQELSTSENDGPISETFRKGKNVDALILYFGEDPSRCQFEQASWCMNSSLVLGCIYMNFQNFNVKICLSVAS

Target ORF information:

RefSeq Version	XM_024782693.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula formin-like protein 13 (LOC11439065), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024782693.1

ORF Insert Sequence:

ATGGCTATGC TCCGCAAGTT GTTCTATCGT AAGCCTCCTG ATGGTCTCCT CGAGATCTGC 
GACAGAGTTT ACGTTTTTGA TTGCTGTTTC ACAACGGAAG CTTGGAATGA AGAGAAGTAT 
AAAGTGTATA TGGATGGTAT AGTAGGTCAG CTTAGAGAGA ATGTGCCTGA TGCTTCTATT 
TTGGTTTTCA ATTTTCGCGA GGAAGAGACC AAGAGTTTGA TGGCAAATAT AATATCTGAG 
TATGACATTA CTATAATGGA TTATCCTAGG CATTATGAAG GTTGTCCTGT GCTTAAAATG 
GAGCTCATTC ACCACTTTTT AAGGTCTAGT GAAAGTTGGC TTTCACTTGG TCAACACAAT 
GTGTTGTTGA TGCATTGTGA ACGTGGTGGT TGGCCCGTTA TGGCTTTCAT GTTAGCTGCA 
TTGTTGATTT ATCGGAAGGT TTATAGTGGC GAGCATAGGA CTTTGGATAT GATTTATCGG 
CAGTCCCCAC ACCAGCTTTT ACATTTGTTG ACGCCGTTGA ATCCAATTCC TTCCCAACTG 
AGGTATCTGC AGTATGTTTC TAGGAGGAAT GTGGCCTTAG ATTGGCCTCC GCTCGACAGG 
GCACTCATGT TGGACTGTAT TATACTTAGA TTCTTCCCAA ACTTTGATGG GGAAGGTGGT 
TGTCATCCAT TGTTTAGAAT TTATGGACAG GATCCTTTTA GTGCAGATAA AAGTCCTAAA 
ATGCTGTACT CAATGCCAAA GAGAAGTAAA AATGTCCGGG CTTACAAGCA GGGAGAGTGC 
GAACTAATTA AAATTGATAT CAATTGCCAT ATTCAAGGTG ATGTTGTGAT CGAGGGTATT 
AACTTGAATG ATGATATGGA GCATGAAATG ATGATGTTCC GAGTTATGTT CAACACAGCT 
TTTGTTAGAT CCAATATTCT GATGCTTAAT CGCGATGAAA TTGATGTTCT ATGGGATGCT 
AAAGATCACT TTCCAAAAGA TTTCAGAGCT GAGATCCTTT TCTCAGAGAT AGATGCTGCT 
GCAGCAGTTA TTTTAGATAA TACATCATTC TTTGAGGAGA AAGATGGACT GCCAATTGAA 
GCATTTGCCA AGGTTCAAGA GATCTTTAGC CATGTGGACT GGATGAACCC AAAGGCAGAT 
GCTGCACTAA ACGTGCTCCA ACAAATAAGT ACTTCTGCTA TCATGAATGA CAAGCTGGAT 
AAAGTTTCTG ACCAGCATGT GGAAACTGCC ACTTCCTTGG GCGAAACAGG TCCTAAAACG 
CCTCAAAGGA ATTCATATGC AGCAATACGG TCTTTATCCT CAACTAAGAG AACCCCAAAT 
AATGATATGA GCAGAAAAGA GGAAAAGACA AACAAAGTAG ATTCTATTCC ACAGAGGGCT 
AATACATCAG ACATCATCGG TCAGGAGAAA ATTTGCTCAT CTGAAAAAAG TTTGGAATCC 
AGTAAATGTC CAACAGGATC AACAAATTTT GACATAAAAC CACAGGAATC TAATCTTGCT 
TCATCTAGTT CTGCAGATTC TTCTCTTTCC CCTGGAACAC CTCCTCCGCG GCCTCCCTTG 
ACTACTCGTT CTAAAGAAGT TCATGATTCT CCTCCCCACA CAGAATTGCC TCCACATCAT 
ATTTTGCCTT CGCAATCAGG ACCTCAATCA CAAGACAGGA GTTACTCACC TATTTCTAGC 
TCTACACCTG AGACCTATCA TTCCCTTGCA CCTGATTCTT CAATTGAGCC ATCCCCTCCA 
CTTTCTTCGA GAAAACCTTT GAATGACATT CCACCAGTCA AAACAAGACC CAAGTCTCCC 
TCTTCGCAGC CTCCAACTCC ACCTCCTCCT CCTACCCCTC CACTAAAAGA TCAGAAAGTA 
GTTAGGGCTG GACCTCTCTC CCCACCCCCA CCACCTCCTT CTCCAAAGAA GGATATACAT 
GTTAAAGCTG GACCTCCTCC TTCTCCTCCT GTCCCATCTC ATATGAATGA GAAACCACAT 
GTTAGAGATG GACTTTCTCC TCCCCCTCCC CCTCTTCCCC CATCACCTCC TCTAAAAGCG 
GAGCAACCTA CTAGGTTTCA GCCCCCGCCA CCTCCACCAC CACCTCGTCT TTCAGTGGAA 
GCTGCAAGTC CTATTACAGC ACCGCCTGCA CCGCCTCCAC CTCCATCTGC AACACTTTCC 
TCTGGTAACC CTAATGCTTC ATTGCAAAAG TCTTCACCTG CTCCACCACC TCCAATTCCT 
TTTGGTAAAG GTCTAAAACC AGGCAGTGCT TTTCCAATGT CCCTTTCTGT GGGAGTTGAT 
GGAAATAAGG TCTCAGGACC TCAATCTAGT TCACTAGCAG GCTCAAAGGG ACGAGTTCTA 
CCACGTGCAA TTGGCTCAAA AAATGACTCC AAAAAGTTGA AGCCATTGCA TTGGATGAAG 
CTATCTAGAG CAGTGCAAGG AAGTTTGTGG GATGAGACAC AAAAATCTGG CGAAGCTTCC 
AAAGCCCCAG AGATTGATAT GTCAGAGCTC GAGAGTCTCT TCTCGGCAGC AGCCCCTAGT 
TCAGGCCCTG CCAAGAAGTC AAATGTCCAA AGCTCAGTGA AGCCTAAATC TGAGAAAGTG 
CAACTGATTG ATCATAGGCG AGCATATAAT TGTGAAATCA TGCTTTCAAA AGTGAAGGTG 
CCATTGCATG ATTTAATGAG CTCAGTGCTA GCACTAGAAG AGTCGGCACT AGACACTGAT 
ACTGTCGAGA ACCTTATAAA GTTCTGTCCT ACAAAAGAGG AGATGGAGAT AATTAAGAAT 
TATAACGGGG AAAAGGAGAA ATTAGGGAGA TGTGAACAGT TCTTCATGGA GTTGATGAAA 
GTACCACGGG TAGAAGCTAA GCTCAGGGTC TTTTCATTCA GGATTCAGTT CTATTCTCAG 
GTTTCTGATC TTAAAAATAG CCTAAAAGTT GTGAATTCTT CTGCAGAAGA GATCAGGAAT 
TCTGTCAAAT TGAAGAGAAT TATGCAAACA ATACTTACTC TAGGAAATGC TTTGAATCAA 
GGAACTGCCA GGGGTTCTGC TATTGGATTC AGATTAGATA GCCTTCTTAA ACTCACCGAG 
ACACGGGCAC GGAACAACAA AATGACTCTC ATGCATTATC TTTGTAAGGT GTTAGATGAC 
AAATTACCAG AAGTCTTAGA CTTCTCTAAG GATCTTGCTA ACCTAGAGCC AGCAGCAAAG 
ATCCAATTGA AGTTTCTTGC AGAGGAGATG CAAGCTGTAA ACAAAGGGTT AGAGAAAGTC 
GTCCAGGAAT TATCCACTTC TGAAAACGAT GGTCCTATAT CAGAAACGTT CCGCAAGGGC 
AAGAACGTGG ATGCGTTGAT TCTTTATTTT GGGGAAGATC CATCTCGCTG TCAATTTGAG 
CAAGCAAGTT GGTGCATGAA TTCATCACTT GTGCTTGGCT GCATATATAT GAATTTTCAA 
AATTTCAATG TCAAAATTTG TCTATCTGTA GCTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11439065 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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