Ankfy1 cDNA ORF clone, Marmota marmota marmota(Alpine marmot)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankfy1
Entrez Gene ID	107159370
Full Name	ankyrin repeat and FYVE domain containing 1
Gene Type	protein-coding
Organism	Marmota marmota marmota(Alpine marmot)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015505939.1	XP_015361425.1	rabankyrin-5 isoform X1
XM_015505940.1	XP_015361426.1	rabankyrin-5 isoform X2
XM_015505941.1	XP_015361427.1	rabankyrin-5 isoform X3

Pathways from BioSystems
Gene Ontology

The following Ankfy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankfy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa109224	XM_015505939.1 Latest version!	Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMa109225	XM_015505940.1 Latest version!	Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMa109226	XM_015505941.1 Latest version!	Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankfy1 ( XM_015505939.1 ) cDNA ORF clone, Marmota marmota marmota(Alpine marmot) -> XP_015361425.1 Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa109224
Clone ID Related Accession (Same CDS sequence)	XM_015505939.1
Accession Version	XM_015505939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-27
Organism	Marmota marmota marmota(Alpine marmot)
Product	rabankyrin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015351318.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Marmota marmota marmota Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG AGGAGGTGTC CAAGTTGGAG AAGCACTTGA TGCTTCTCCG GCAAGAGTAT 
GTCAAGCTGC AGAAGAAATT GGCAGAGACA GAGAAGAGAT GCACTCTTTT GGCTGCACAA 
GCAAACAAGG AAAACAGCAG TGAGTCCTTC ATCAGCCGGT TGCTGACGAT AGTGGCGGAC 
CTGTATGAGC AAGAGCAGTA CAGTGATCTG AAAATAAAGG TTGGAGGCAG GCACATCAGT 
GCTCATAAGT TTGTCCTGGC AGCCCGTAGT GACAGCTGGA GTCTGGCCAA CTTGTCTTCC 
ACTGAGGAAC TGGACCTGTC AGATGCTAAT CCTGAGGTGA CAATGACAAT GCTTCGCTGG 
ATCTACACAG ATGAGCTGGA GTTCAGAGAG GATGATGTGT TCCTGACTGA ATTGATGAAA 
CTAGCAAATC GGTTTCAGCT ACAGCTCCTT AGGGAGAGAT GTGAAAAGGG TGTTATGTCC 
CTGGTGAATG TTAGGAACTG TATTCGCTTC TATCAGACTG CAGAGGAACT GAATGCCAGC 
ACACTGATGA ACTACTGTGC AGAGATTATT GCCAGTCATT GGGATGACTT ACGAAAGGAG 
GACTTTAGCA GTATGGGTGC TCAGTTGTTA TACAAAATGA TCAAATCCAA GACAGAGTAC 
CCATTACACA AAGCTATCAA AGTGGAGAGA GAAGATGTGG TCTTCCTTTA TCTGATTGAA 
ATGGATTCAC AGCTCCCTGG GAAGCTAAAT GAAGTGGATC ATAATGGAGA TCTTGCATTA 
GATCTAGCCC TTTCACGACG ACTAGAGAGT ATTGCTACCA CGCTGGTTAG TCATAAAGCT 
GATGTGGACA TGGTAGACAA GAATGGCTGG AGCTTGTTAC ATAAAGGAAT CCAAAGAGGA 
GATCTCTTTG CTTCTACTTT CCTTATTAAG AATGGGGCCC TTGTTAATGC TGCTACATTG 
GGTGCCCAAG AGACACCATT ACACCTTGTG GCATTGTACA GTTCAAAGAA ACATTCAACA 
GATGTGATGT CTGAGATGGC ACAGATTGCA GAGGCCCTCC TACAGGCTGG TGCCAATCCC 
AACATGCAGG ATAGCAAGGG CAGGACTCCT TTACACCTGT CCATCACCGC CAGGAATGAA 
TATGTGTTCA ATCAGCTGCT GCAGTGTAAA CAGTTAGATT TAGAGCTAAA AGACCATGAA 
GGCAGCACAG CTTTGTGGCT GGCAGTACAG TACATCACTG TATCTTCGGA TGCGTCTGTA 
AACCCCTTTG AAGACCTCCC TGTGGTAAAT GGGACTTCAT TTGATGAAAA CAGCTTTGCA 
GCCAGACTCA TTCAGCGTGG CAGCAACACT GATGCACCTG ATACCATGAC AGGAAATTGT 
TTACTGCAGC GGGCAGCTGG AGCAGGAAAT GAGGCAGCAG CACTTTTCCT GGCGACCAAT 
GGTGCCCATG TCAACCACAA AAACAAGTGG GGAGAAACCC CATTGCACAC AGCTTGCCGG 
CACGGCCTGG CCAACCTCAC AGCAGAGCTC CTGCAGCAAG GTGCCAACCC AAACCTGCAG 
ACAGAGGAGG CTCTGCCTTT GCCAAAGGAG GTCACATCCC TAACCAGCTC CATGGACAAT 
GTGTATCTGC AGACGCCGCT TCACATGGCA ATTGCTTATA ATCACCCAGA TGTGGTATCT 
GTCATCCTGG AGCAGAAAGC CAATGCTCTT CATGCCACCA ACAACTTGCA AATTATTCCG 
GACTTCAGCC TCAAGGATTC CCGGGACCAG ACTGTGCTGG GCCTGGCATT GTGGACTGGC 
ATGCATACCA TTGCAGCCCA GCTGCTGGGC TCCGGGGCTT CCATCAATGA CACCATGTCA 
GATGGGCAAA CCTTGTTGCA CATGGCCATA CAGCGGCAGG ATAGCAAGAG TGCACTCTTT 
CTCCTGGAGC ATCAGGCAGA TATAAATGTC AGCAGGACTC AGGATGGGGA GACAGCCCTT 
CAGCTGGCCA TCAGAAATCA GCTTCCGCTC GTAGTTGATG CCATATGCAC CCGAGGAGCT 
GACATGTCCG TTTCAGACGA GAAAGGGAAC CCCCCACTGT GGCTTGCGTT GGCAAATAAT 
TTGGAAGACA TTGCATCCAC TCTGGTCAGA CATGGTTGTG ATGCCACATG CTGGGGTCCA 
GGACCTAGCG GTTGCCTCCA AACACTTCTG CACAGAGCTA TTGATGAAAA CAATGAGTCC 
ACTGCCTGCT TTCTTATTCG CAGTGGCTGT GATGTGAATA GTCCCAGACA GCCAGGTGCT 
AATGGAGAAG GAGAGGAAGA AGCCAGGGAT GGGCAGACTC CCTTGCATTT GGCAGCTTCC 
TGGGGACTGG AAGAGACTGT GCAGTGTCTT CTGGAATTTG GTGCCAATGT AAATGCACAG 
GATGCAGAAG GCAGAACACC TGTCCATGTG GCCATCAGCA ACCAACACAG TGTCATCATT 
CAGTTGTTGA TTTCTCACCC TGATATCCAC TTGAATGTAC GAGACAGGCA AGGGCTGACC 
CCATTTGCCT GTGCCATGAC TTACAAGAAC AACAAGGCAG CTGAGGCCAT TCTAAAACGA 
GAGTCTGGAG CTGCTGAGCA GGTGGATAAC AAGGGCCGGA ATTTTCTTCA CGTGGCAGTT 
CAGAACTCTG ATATTGAAAG TGTCCTGTTC TTGATCAGTG TCCAGGCTAA TGTGAATTCC 
AGAGTCCAGG ATGCTTCCAA GTTGACTCCT TTGCATCTTG CTGTCCAAGC AGGCTCAGAG 
ATTATTGTTC GCAATTTGCT TCTTGCAGGA GCCAAGGTGA ATGAATTAAC CAAGCATCGC 
CAAACTGCCC TCCATCTTGC TGCCCAGCAG GACCTGCCTA CCATCTGCTC AGTTCTCCTA 
GAGAATGGAG TAGACTTTGC TGCTGTGGAT GAGAATGGAA ATAATGCTCT TCATCTTGCT 
GTCATGCATG GTCGACTCAA CAACATCCGG GTTCTCCTGA CAGAGTGCAC AGTGGATGCT 
GAAGCCTTTA ATCTGAGAGG CCAGTCACCA CTGCATATTT TGGGACAGTA TGGCAAAGAG 
AATGCAGCAG CCATCTTTGA TCTGTTCTTA GAGTGCATGC CTGAGTATCC TCTAGATAAA 
CCAGATGCAG AAGGAAACAC AGTGCTGCTC TTAGCATACA TGAAAGGGAA TGCCAACTTG 
TGCCGTGCCA TCGTCCGATC TGGGGCTCGT CTTGGGGTGA ATAATAACCA AGGTGTGAAT 
ATTTTCAACT ACCAGGTTGC AACCAAGCAA CTTCTGTTTA GACTATTAGA CATGCTCTCC 
AAGGAACCTC CATGGTGTGA TGGCTCCAAC TGCTACGAGT GCACTGCCAA GTTTGGAGTC 
ACAACGCGCA AGCATCACTG TCGGCATTGT GGACGTCTGC TTTGCCATAA ATGTTCTACC 
AAGGAAATTC CTATTATAAA GTTTGATCTG AACAAGCCTG TGCGAGTCTG CAACATTTGC 
TTTGATGTAC TGACACTGGG TGGAGTCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015361425.1
CDS	23..3535
Translation	MAEEEVSKLEKHLMLLRQEYVKLQKKLAETEKRCTLLAAQANKENSSESFISRLLTIVADLYEQEQYSDLKIKVGGRHISAHKFVLAARSDSWSLANLSSTEELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMGAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEVDHNGDLALDLALSRRLESIATTLVSHKADVDMVDKNGWSLLHKGIQRGDLFASTFLIKNGALVNAATLGAQETPLHLVALYSSKKHSTDVMSEMAQIAEALLQAGANPNMQDSKGRTPLHLSITARNEYVFNQLLQCKQLDLELKDHEGSTALWLAVQYITVSSDASVNPFEDLPVVNGTSFDENSFAARLIQRGSNTDAPDTMTGNCLLQRAAGAGNEAAALFLATNGAHVNHKNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEVTSLTSSMDNVYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGASINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVSRTQDGETALQLAIRNQLPLVVDAICTRGADMSVSDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAIDENNESTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPDIHLNVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVQANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPEYPLDKPDAEGNTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSNCYECTAKFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS

Target ORF information:

RefSeq Version	XM_015505939.1
Organism	Marmota marmota marmota(Alpine marmot)
Definition	Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015505939.1

ORF Insert Sequence:

ATGGCGGAAG AGGAGGTGTC CAAGTTGGAG AAGCACTTGA TGCTTCTCCG GCAAGAGTAT 
GTCAAGCTGC AGAAGAAATT GGCAGAGACA GAGAAGAGAT GCACTCTTTT GGCTGCACAA 
GCAAACAAGG AAAACAGCAG TGAGTCCTTC ATCAGCCGGT TGCTGACGAT AGTGGCGGAC 
CTGTATGAGC AAGAGCAGTA CAGTGATCTG AAAATAAAGG TTGGAGGCAG GCACATCAGT 
GCTCATAAGT TTGTCCTGGC AGCCCGTAGT GACAGCTGGA GTCTGGCCAA CTTGTCTTCC 
ACTGAGGAAC TGGACCTGTC AGATGCTAAT CCTGAGGTGA CAATGACAAT GCTTCGCTGG 
ATCTACACAG ATGAGCTGGA GTTCAGAGAG GATGATGTGT TCCTGACTGA ATTGATGAAA 
CTAGCAAATC GGTTTCAGCT ACAGCTCCTT AGGGAGAGAT GTGAAAAGGG TGTTATGTCC 
CTGGTGAATG TTAGGAACTG TATTCGCTTC TATCAGACTG CAGAGGAACT GAATGCCAGC 
ACACTGATGA ACTACTGTGC AGAGATTATT GCCAGTCATT GGGATGACTT ACGAAAGGAG 
GACTTTAGCA GTATGGGTGC TCAGTTGTTA TACAAAATGA TCAAATCCAA GACAGAGTAC 
CCATTACACA AAGCTATCAA AGTGGAGAGA GAAGATGTGG TCTTCCTTTA TCTGATTGAA 
ATGGATTCAC AGCTCCCTGG GAAGCTAAAT GAAGTGGATC ATAATGGAGA TCTTGCATTA 
GATCTAGCCC TTTCACGACG ACTAGAGAGT ATTGCTACCA CGCTGGTTAG TCATAAAGCT 
GATGTGGACA TGGTAGACAA GAATGGCTGG AGCTTGTTAC ATAAAGGAAT CCAAAGAGGA 
GATCTCTTTG CTTCTACTTT CCTTATTAAG AATGGGGCCC TTGTTAATGC TGCTACATTG 
GGTGCCCAAG AGACACCATT ACACCTTGTG GCATTGTACA GTTCAAAGAA ACATTCAACA 
GATGTGATGT CTGAGATGGC ACAGATTGCA GAGGCCCTCC TACAGGCTGG TGCCAATCCC 
AACATGCAGG ATAGCAAGGG CAGGACTCCT TTACACCTGT CCATCACCGC CAGGAATGAA 
TATGTGTTCA ATCAGCTGCT GCAGTGTAAA CAGTTAGATT TAGAGCTAAA AGACCATGAA 
GGCAGCACAG CTTTGTGGCT GGCAGTACAG TACATCACTG TATCTTCGGA TGCGTCTGTA 
AACCCCTTTG AAGACCTCCC TGTGGTAAAT GGGACTTCAT TTGATGAAAA CAGCTTTGCA 
GCCAGACTCA TTCAGCGTGG CAGCAACACT GATGCACCTG ATACCATGAC AGGAAATTGT 
TTACTGCAGC GGGCAGCTGG AGCAGGAAAT GAGGCAGCAG CACTTTTCCT GGCGACCAAT 
GGTGCCCATG TCAACCACAA AAACAAGTGG GGAGAAACCC CATTGCACAC AGCTTGCCGG 
CACGGCCTGG CCAACCTCAC AGCAGAGCTC CTGCAGCAAG GTGCCAACCC AAACCTGCAG 
ACAGAGGAGG CTCTGCCTTT GCCAAAGGAG GTCACATCCC TAACCAGCTC CATGGACAAT 
GTGTATCTGC AGACGCCGCT TCACATGGCA ATTGCTTATA ATCACCCAGA TGTGGTATCT 
GTCATCCTGG AGCAGAAAGC CAATGCTCTT CATGCCACCA ACAACTTGCA AATTATTCCG 
GACTTCAGCC TCAAGGATTC CCGGGACCAG ACTGTGCTGG GCCTGGCATT GTGGACTGGC 
ATGCATACCA TTGCAGCCCA GCTGCTGGGC TCCGGGGCTT CCATCAATGA CACCATGTCA 
GATGGGCAAA CCTTGTTGCA CATGGCCATA CAGCGGCAGG ATAGCAAGAG TGCACTCTTT 
CTCCTGGAGC ATCAGGCAGA TATAAATGTC AGCAGGACTC AGGATGGGGA GACAGCCCTT 
CAGCTGGCCA TCAGAAATCA GCTTCCGCTC GTAGTTGATG CCATATGCAC CCGAGGAGCT 
GACATGTCCG TTTCAGACGA GAAAGGGAAC CCCCCACTGT GGCTTGCGTT GGCAAATAAT 
TTGGAAGACA TTGCATCCAC TCTGGTCAGA CATGGTTGTG ATGCCACATG CTGGGGTCCA 
GGACCTAGCG GTTGCCTCCA AACACTTCTG CACAGAGCTA TTGATGAAAA CAATGAGTCC 
ACTGCCTGCT TTCTTATTCG CAGTGGCTGT GATGTGAATA GTCCCAGACA GCCAGGTGCT 
AATGGAGAAG GAGAGGAAGA AGCCAGGGAT GGGCAGACTC CCTTGCATTT GGCAGCTTCC 
TGGGGACTGG AAGAGACTGT GCAGTGTCTT CTGGAATTTG GTGCCAATGT AAATGCACAG 
GATGCAGAAG GCAGAACACC TGTCCATGTG GCCATCAGCA ACCAACACAG TGTCATCATT 
CAGTTGTTGA TTTCTCACCC TGATATCCAC TTGAATGTAC GAGACAGGCA AGGGCTGACC 
CCATTTGCCT GTGCCATGAC TTACAAGAAC AACAAGGCAG CTGAGGCCAT TCTAAAACGA 
GAGTCTGGAG CTGCTGAGCA GGTGGATAAC AAGGGCCGGA ATTTTCTTCA CGTGGCAGTT 
CAGAACTCTG ATATTGAAAG TGTCCTGTTC TTGATCAGTG TCCAGGCTAA TGTGAATTCC 
AGAGTCCAGG ATGCTTCCAA GTTGACTCCT TTGCATCTTG CTGTCCAAGC AGGCTCAGAG 
ATTATTGTTC GCAATTTGCT TCTTGCAGGA GCCAAGGTGA ATGAATTAAC CAAGCATCGC 
CAAACTGCCC TCCATCTTGC TGCCCAGCAG GACCTGCCTA CCATCTGCTC AGTTCTCCTA 
GAGAATGGAG TAGACTTTGC TGCTGTGGAT GAGAATGGAA ATAATGCTCT TCATCTTGCT 
GTCATGCATG GTCGACTCAA CAACATCCGG GTTCTCCTGA CAGAGTGCAC AGTGGATGCT 
GAAGCCTTTA ATCTGAGAGG CCAGTCACCA CTGCATATTT TGGGACAGTA TGGCAAAGAG 
AATGCAGCAG CCATCTTTGA TCTGTTCTTA GAGTGCATGC CTGAGTATCC TCTAGATAAA 
CCAGATGCAG AAGGAAACAC AGTGCTGCTC TTAGCATACA TGAAAGGGAA TGCCAACTTG 
TGCCGTGCCA TCGTCCGATC TGGGGCTCGT CTTGGGGTGA ATAATAACCA AGGTGTGAAT 
ATTTTCAACT ACCAGGTTGC AACCAAGCAA CTTCTGTTTA GACTATTAGA CATGCTCTCC 
AAGGAACCTC CATGGTGTGA TGGCTCCAAC TGCTACGAGT GCACTGCCAA GTTTGGAGTC 
ACAACGCGCA AGCATCACTG TCGGCATTGT GGACGTCTGC TTTGCCATAA ATGTTCTACC 
AAGGAAATTC CTATTATAAA GTTTGATCTG AACAAGCCTG TGCGAGTCTG CAACATTTGC 
TTTGATGTAC TGACACTGGG TGGAGTCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfy1 ( XM_015505940.1 ) cDNA ORF clone, Marmota marmota marmota(Alpine marmot) -> XP_015361426.1 Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa109225
Clone ID Related Accession (Same CDS sequence)	XM_015505940.1
Accession Version	XM_015505940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-27
Organism	Marmota marmota marmota(Alpine marmot)
Product	rabankyrin-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015351318.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Marmota marmota marmota Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG AGGAGGTGTC CAAGTTGGAG AAGCACTTGA TGCTTCTCCG GCAAGAGTAT 
GTCAAGCTGC AGAAGAAATT GGCAGAGACA GAGAAGAGAT GCACTCTTTT GGCTGCACAA 
GCAAACAAGG AAAACAGCAG TGAGTCCTTC ATCAGCCGGT TGCTGACGAT AGTGGCGGAC 
CTGTATGAGC AAGAGCAGTA CAGTGATCTG AAAATAAAGG TTGGAGGCAG GCACATCAGT 
GCTCATAAGT TTGTCCTGGC AGCCCGTAGT GACAGCTGGA GTCTGGCCAA CTTGTCTTCC 
ACTGAGGAAC TGGACCTGTC AGATGCTAAT CCTGAGGTGA CAATGACAAT GCTTCGCTGG 
ATCTACACAG ATGAGCTGGA GTTCAGAGAG GATGATGTGT TCCTGACTGA ATTGATGAAA 
CTAGCAAATC GGTTTCAGCT ACAGCTCCTT AGGGAGAGAT GTGAAAAGGG TGTTATGTCC 
CTGGTGAATG TTAGGAACTG TATTCGCTTC TATCAGACTG CAGAGGAACT GAATGCCAGC 
ACACTGATGA ACTACTGTGC AGAGATTATT GCCAGTCATT GGGATGACTT ACGAAAGGAG 
GACTTTAGCA GTATGGGTGC TCAGTTGTTA TACAAAATGA TCAAATCCAA GACAGAGTAC 
CCATTACACA AAGCTATCAA AGTGGAGAGA GAAGATGTGG TCTTCCTTTA TCTGATTGAA 
ATGGATTCAC AGCTCCCTGG GAAGCTAAAT GAAGTGGATC ATAATGGAGA TCTTGCATTA 
GATCTAGCCC TTTCACGACG ACTAGAGAGT ATTGCTACCA CGCTGGTTAG TCATAAAGCT 
GATGTGGACA TGGTAGACAA GAATGGCTGG AGCTTGTTAC ATAAAGGAAT CCAAAGAGGA 
GATCTCTTTG CTTCTACTTT CCTTATTAAG AATGGGGCCC TTGTTAATGC TGCTACATTG 
GGTGCCCAAG AGACACCATT ACACCTTGTG GCATTGTACA GTTCAAAGAA ACATTCAACA 
GATGTGATGT CTGAGATGGC ACAGATTGCA GAGGCCCTCC TACAGGCTGG TGCCAATCCC 
AACATGCAGG ATAGCAAGGG CAGGACTCCT TTACACCTGT CCATCACCGC CAGGAATGAA 
TATGTGTTCA ATCAGCTGCT GCAGTGTAAA CAGTTAGATT TAGAGCTAAA AGACCATGAA 
GGCAGCACAG CTTTGTGGCT GGCAGTACAG TACATCACTG TATCTTCGGA TGCGTCTGTA 
AACCCCTTTG AAGACCTCCC TGTGGTAAAT GGGACTTCAT TTGATGAAAA CAGCTTTGCA 
GCCAGACTCA TTCAGCGTGG CAGCAACACT GATGCACCTG ATACCATGAC AGGAAATTGT 
TTACTGCAGC GGGCAGCTGG AGCAGGAAAT GAGGCAGCAG CACTTTTCCT GGCGACCAAT 
GGTGCCCATG TCAACCACAA AAACAAGTGG GGAGAAACCC CATTGCACAC AGCTTGCCGG 
CACGGCCTGG CCAACCTCAC AGCAGAGCTC CTGCAGCAAG GTGCCAACCC AAACCTGCAG 
ACAGAGGAGG CTCTGCCTTT GCCAAAGGAG GTCACATCCC TAACCAGCTC CATGGACAAT 
GTGTATCTGC AGACGCCGCT TCACATGGCA ATTGCTTATA ATCACCCAGA TGTGGTATCT 
GTCATCCTGG AGCAGAAAGC CAATGCTCTT CATGCCACCA ACAACTTGCA AATTATTCCG 
GACTTCAGCC TCAAGGATTC CCGGGACCAG ACTGTGCTGG GCCTGGCATT GTGGACTGGC 
ATGCATACCA TTGCAGCCCA GCTGCTGGGC TCCGGGGCTT CCATCAATGA CACCATGTCA 
GATGGGCAAA CCTTGTTGCA CATGGCCATA CAGCGGCAGG ATAGCAAGAG TGCACTCTTT 
CTCCTGGAGC ATCAGGCAGA TATAAATGTC AGGACTCAGG ATGGGGAGAC AGCCCTTCAG 
CTGGCCATCA GAAATCAGCT TCCGCTCGTA GTTGATGCCA TATGCACCCG AGGAGCTGAC 
ATGTCCGTTT CAGACGAGAA AGGGAACCCC CCACTGTGGC TTGCGTTGGC AAATAATTTG 
GAAGACATTG CATCCACTCT GGTCAGACAT GGTTGTGATG CCACATGCTG GGGTCCAGGA 
CCTAGCGGTT GCCTCCAAAC ACTTCTGCAC AGAGCTATTG ATGAAAACAA TGAGTCCACT 
GCCTGCTTTC TTATTCGCAG TGGCTGTGAT GTGAATAGTC CCAGACAGCC AGGTGCTAAT 
GGAGAAGGAG AGGAAGAAGC CAGGGATGGG CAGACTCCCT TGCATTTGGC AGCTTCCTGG 
GGACTGGAAG AGACTGTGCA GTGTCTTCTG GAATTTGGTG CCAATGTAAA TGCACAGGAT 
GCAGAAGGCA GAACACCTGT CCATGTGGCC ATCAGCAACC AACACAGTGT CATCATTCAG 
TTGTTGATTT CTCACCCTGA TATCCACTTG AATGTACGAG ACAGGCAAGG GCTGACCCCA 
TTTGCCTGTG CCATGACTTA CAAGAACAAC AAGGCAGCTG AGGCCATTCT AAAACGAGAG 
TCTGGAGCTG CTGAGCAGGT GGATAACAAG GGCCGGAATT TTCTTCACGT GGCAGTTCAG 
AACTCTGATA TTGAAAGTGT CCTGTTCTTG ATCAGTGTCC AGGCTAATGT GAATTCCAGA 
GTCCAGGATG CTTCCAAGTT GACTCCTTTG CATCTTGCTG TCCAAGCAGG CTCAGAGATT 
ATTGTTCGCA ATTTGCTTCT TGCAGGAGCC AAGGTGAATG AATTAACCAA GCATCGCCAA 
ACTGCCCTCC ATCTTGCTGC CCAGCAGGAC CTGCCTACCA TCTGCTCAGT TCTCCTAGAG 
AATGGAGTAG ACTTTGCTGC TGTGGATGAG AATGGAAATA ATGCTCTTCA TCTTGCTGTC 
ATGCATGGTC GACTCAACAA CATCCGGGTT CTCCTGACAG AGTGCACAGT GGATGCTGAA 
GCCTTTAATC TGAGAGGCCA GTCACCACTG CATATTTTGG GACAGTATGG CAAAGAGAAT 
GCAGCAGCCA TCTTTGATCT GTTCTTAGAG TGCATGCCTG AGTATCCTCT AGATAAACCA 
GATGCAGAAG GAAACACAGT GCTGCTCTTA GCATACATGA AAGGGAATGC CAACTTGTGC 
CGTGCCATCG TCCGATCTGG GGCTCGTCTT GGGGTGAATA ATAACCAAGG TGTGAATATT 
TTCAACTACC AGGTTGCAAC CAAGCAACTT CTGTTTAGAC TATTAGACAT GCTCTCCAAG 
GAACCTCCAT GGTGTGATGG CTCCAACTGC TACGAGTGCA CTGCCAAGTT TGGAGTCACA 
ACGCGCAAGC ATCACTGTCG GCATTGTGGA CGTCTGCTTT GCCATAAATG TTCTACCAAG 
GAAATTCCTA TTATAAAGTT TGATCTGAAC AAGCCTGTGC GAGTCTGCAA CATTTGCTTT 
GATGTACTGA CACTGGGTGG AGTCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015361426.1
CDS	29..3538
Translation	MAEEEVSKLEKHLMLLRQEYVKLQKKLAETEKRCTLLAAQANKENSSESFISRLLTIVADLYEQEQYSDLKIKVGGRHISAHKFVLAARSDSWSLANLSSTEELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMGAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEVDHNGDLALDLALSRRLESIATTLVSHKADVDMVDKNGWSLLHKGIQRGDLFASTFLIKNGALVNAATLGAQETPLHLVALYSSKKHSTDVMSEMAQIAEALLQAGANPNMQDSKGRTPLHLSITARNEYVFNQLLQCKQLDLELKDHEGSTALWLAVQYITVSSDASVNPFEDLPVVNGTSFDENSFAARLIQRGSNTDAPDTMTGNCLLQRAAGAGNEAAALFLATNGAHVNHKNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEVTSLTSSMDNVYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGASINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVSDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAIDENNESTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPDIHLNVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVQANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPEYPLDKPDAEGNTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSNCYECTAKFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS

Target ORF information:

RefSeq Version	XM_015505940.1
Organism	Marmota marmota marmota(Alpine marmot)
Definition	Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015505940.1

ORF Insert Sequence:

ATGGCGGAAG AGGAGGTGTC CAAGTTGGAG AAGCACTTGA TGCTTCTCCG GCAAGAGTAT 
GTCAAGCTGC AGAAGAAATT GGCAGAGACA GAGAAGAGAT GCACTCTTTT GGCTGCACAA 
GCAAACAAGG AAAACAGCAG TGAGTCCTTC ATCAGCCGGT TGCTGACGAT AGTGGCGGAC 
CTGTATGAGC AAGAGCAGTA CAGTGATCTG AAAATAAAGG TTGGAGGCAG GCACATCAGT 
GCTCATAAGT TTGTCCTGGC AGCCCGTAGT GACAGCTGGA GTCTGGCCAA CTTGTCTTCC 
ACTGAGGAAC TGGACCTGTC AGATGCTAAT CCTGAGGTGA CAATGACAAT GCTTCGCTGG 
ATCTACACAG ATGAGCTGGA GTTCAGAGAG GATGATGTGT TCCTGACTGA ATTGATGAAA 
CTAGCAAATC GGTTTCAGCT ACAGCTCCTT AGGGAGAGAT GTGAAAAGGG TGTTATGTCC 
CTGGTGAATG TTAGGAACTG TATTCGCTTC TATCAGACTG CAGAGGAACT GAATGCCAGC 
ACACTGATGA ACTACTGTGC AGAGATTATT GCCAGTCATT GGGATGACTT ACGAAAGGAG 
GACTTTAGCA GTATGGGTGC TCAGTTGTTA TACAAAATGA TCAAATCCAA GACAGAGTAC 
CCATTACACA AAGCTATCAA AGTGGAGAGA GAAGATGTGG TCTTCCTTTA TCTGATTGAA 
ATGGATTCAC AGCTCCCTGG GAAGCTAAAT GAAGTGGATC ATAATGGAGA TCTTGCATTA 
GATCTAGCCC TTTCACGACG ACTAGAGAGT ATTGCTACCA CGCTGGTTAG TCATAAAGCT 
GATGTGGACA TGGTAGACAA GAATGGCTGG AGCTTGTTAC ATAAAGGAAT CCAAAGAGGA 
GATCTCTTTG CTTCTACTTT CCTTATTAAG AATGGGGCCC TTGTTAATGC TGCTACATTG 
GGTGCCCAAG AGACACCATT ACACCTTGTG GCATTGTACA GTTCAAAGAA ACATTCAACA 
GATGTGATGT CTGAGATGGC ACAGATTGCA GAGGCCCTCC TACAGGCTGG TGCCAATCCC 
AACATGCAGG ATAGCAAGGG CAGGACTCCT TTACACCTGT CCATCACCGC CAGGAATGAA 
TATGTGTTCA ATCAGCTGCT GCAGTGTAAA CAGTTAGATT TAGAGCTAAA AGACCATGAA 
GGCAGCACAG CTTTGTGGCT GGCAGTACAG TACATCACTG TATCTTCGGA TGCGTCTGTA 
AACCCCTTTG AAGACCTCCC TGTGGTAAAT GGGACTTCAT TTGATGAAAA CAGCTTTGCA 
GCCAGACTCA TTCAGCGTGG CAGCAACACT GATGCACCTG ATACCATGAC AGGAAATTGT 
TTACTGCAGC GGGCAGCTGG AGCAGGAAAT GAGGCAGCAG CACTTTTCCT GGCGACCAAT 
GGTGCCCATG TCAACCACAA AAACAAGTGG GGAGAAACCC CATTGCACAC AGCTTGCCGG 
CACGGCCTGG CCAACCTCAC AGCAGAGCTC CTGCAGCAAG GTGCCAACCC AAACCTGCAG 
ACAGAGGAGG CTCTGCCTTT GCCAAAGGAG GTCACATCCC TAACCAGCTC CATGGACAAT 
GTGTATCTGC AGACGCCGCT TCACATGGCA ATTGCTTATA ATCACCCAGA TGTGGTATCT 
GTCATCCTGG AGCAGAAAGC CAATGCTCTT CATGCCACCA ACAACTTGCA AATTATTCCG 
GACTTCAGCC TCAAGGATTC CCGGGACCAG ACTGTGCTGG GCCTGGCATT GTGGACTGGC 
ATGCATACCA TTGCAGCCCA GCTGCTGGGC TCCGGGGCTT CCATCAATGA CACCATGTCA 
GATGGGCAAA CCTTGTTGCA CATGGCCATA CAGCGGCAGG ATAGCAAGAG TGCACTCTTT 
CTCCTGGAGC ATCAGGCAGA TATAAATGTC AGGACTCAGG ATGGGGAGAC AGCCCTTCAG 
CTGGCCATCA GAAATCAGCT TCCGCTCGTA GTTGATGCCA TATGCACCCG AGGAGCTGAC 
ATGTCCGTTT CAGACGAGAA AGGGAACCCC CCACTGTGGC TTGCGTTGGC AAATAATTTG 
GAAGACATTG CATCCACTCT GGTCAGACAT GGTTGTGATG CCACATGCTG GGGTCCAGGA 
CCTAGCGGTT GCCTCCAAAC ACTTCTGCAC AGAGCTATTG ATGAAAACAA TGAGTCCACT 
GCCTGCTTTC TTATTCGCAG TGGCTGTGAT GTGAATAGTC CCAGACAGCC AGGTGCTAAT 
GGAGAAGGAG AGGAAGAAGC CAGGGATGGG CAGACTCCCT TGCATTTGGC AGCTTCCTGG 
GGACTGGAAG AGACTGTGCA GTGTCTTCTG GAATTTGGTG CCAATGTAAA TGCACAGGAT 
GCAGAAGGCA GAACACCTGT CCATGTGGCC ATCAGCAACC AACACAGTGT CATCATTCAG 
TTGTTGATTT CTCACCCTGA TATCCACTTG AATGTACGAG ACAGGCAAGG GCTGACCCCA 
TTTGCCTGTG CCATGACTTA CAAGAACAAC AAGGCAGCTG AGGCCATTCT AAAACGAGAG 
TCTGGAGCTG CTGAGCAGGT GGATAACAAG GGCCGGAATT TTCTTCACGT GGCAGTTCAG 
AACTCTGATA TTGAAAGTGT CCTGTTCTTG ATCAGTGTCC AGGCTAATGT GAATTCCAGA 
GTCCAGGATG CTTCCAAGTT GACTCCTTTG CATCTTGCTG TCCAAGCAGG CTCAGAGATT 
ATTGTTCGCA ATTTGCTTCT TGCAGGAGCC AAGGTGAATG AATTAACCAA GCATCGCCAA 
ACTGCCCTCC ATCTTGCTGC CCAGCAGGAC CTGCCTACCA TCTGCTCAGT TCTCCTAGAG 
AATGGAGTAG ACTTTGCTGC TGTGGATGAG AATGGAAATA ATGCTCTTCA TCTTGCTGTC 
ATGCATGGTC GACTCAACAA CATCCGGGTT CTCCTGACAG AGTGCACAGT GGATGCTGAA 
GCCTTTAATC TGAGAGGCCA GTCACCACTG CATATTTTGG GACAGTATGG CAAAGAGAAT 
GCAGCAGCCA TCTTTGATCT GTTCTTAGAG TGCATGCCTG AGTATCCTCT AGATAAACCA 
GATGCAGAAG GAAACACAGT GCTGCTCTTA GCATACATGA AAGGGAATGC CAACTTGTGC 
CGTGCCATCG TCCGATCTGG GGCTCGTCTT GGGGTGAATA ATAACCAAGG TGTGAATATT 
TTCAACTACC AGGTTGCAAC CAAGCAACTT CTGTTTAGAC TATTAGACAT GCTCTCCAAG 
GAACCTCCAT GGTGTGATGG CTCCAACTGC TACGAGTGCA CTGCCAAGTT TGGAGTCACA 
ACGCGCAAGC ATCACTGTCG GCATTGTGGA CGTCTGCTTT GCCATAAATG TTCTACCAAG 
GAAATTCCTA TTATAAAGTT TGATCTGAAC AAGCCTGTGC GAGTCTGCAA CATTTGCTTT 
GATGTACTGA CACTGGGTGG AGTCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankfy1 ( XM_015505941.1 ) cDNA ORF clone, Marmota marmota marmota(Alpine marmot) -> XP_015361427.1 Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X3, mRNA.

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CloneID	OMa109226
Clone ID Related Accession (Same CDS sequence)	XM_015505941.1
Accession Version	XM_015505941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-27
Organism	Marmota marmota marmota(Alpine marmot)
Product	rabankyrin-5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015351318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Marmota marmota marmota Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGG TGAATGTTAG GAACTGTATT CGCTTCTATC AGACTGCAGA GGAACTGAAT 
GCCAGCACAC TGATGAACTA CTGTGCAGAG ATTATTGCCA GTCATTGGGA TGACTTACGA 
AAGGAGGACT TTAGCAGTAT GGGTGCTCAG TTGTTATACA AAATGATCAA ATCCAAGACA 
GAGTACCCAT TACACAAAGC TATCAAAGTG GAGAGAGAAG ATGTGGTCTT CCTTTATCTG 
ATTGAAATGG ATTCACAGCT CCCTGGGAAG CTAAATGAAG TGGATCATAA TGGAGATCTT 
GCATTAGATC TAGCCCTTTC ACGACGACTA GAGAGTATTG CTACCACGCT GGTTAGTCAT 
AAAGCTGATG TGGACATGGT AGACAAGAAT GGCTGGAGCT TGTTACATAA AGGAATCCAA 
AGAGGAGATC TCTTTGCTTC TACTTTCCTT ATTAAGAATG GGGCCCTTGT TAATGCTGCT 
ACATTGGGTG CCCAAGAGAC ACCATTACAC CTTGTGGCAT TGTACAGTTC AAAGAAACAT 
TCAACAGATG TGATGTCTGA GATGGCACAG ATTGCAGAGG CCCTCCTACA GGCTGGTGCC 
AATCCCAACA TGCAGGATAG CAAGGGCAGG ACTCCTTTAC ACCTGTCCAT CACCGCCAGG 
AATGAATATG TGTTCAATCA GCTGCTGCAG TGTAAACAGT TAGATTTAGA GCTAAAAGAC 
CATGAAGGCA GCACAGCTTT GTGGCTGGCA GTACAGTACA TCACTGTATC TTCGGATGCG 
TCTGTAAACC CCTTTGAAGA CCTCCCTGTG GTAAATGGGA CTTCATTTGA TGAAAACAGC 
TTTGCAGCCA GACTCATTCA GCGTGGCAGC AACACTGATG CACCTGATAC CATGACAGGA 
AATTGTTTAC TGCAGCGGGC AGCTGGAGCA GGAAATGAGG CAGCAGCACT TTTCCTGGCG 
ACCAATGGTG CCCATGTCAA CCACAAAAAC AAGTGGGGAG AAACCCCATT GCACACAGCT 
TGCCGGCACG GCCTGGCCAA CCTCACAGCA GAGCTCCTGC AGCAAGGTGC CAACCCAAAC 
CTGCAGACAG AGGAGGCTCT GCCTTTGCCA AAGGAGGTCA CATCCCTAAC CAGCTCCATG 
GACAATGTGT ATCTGCAGAC GCCGCTTCAC ATGGCAATTG CTTATAATCA CCCAGATGTG 
GTATCTGTCA TCCTGGAGCA GAAAGCCAAT GCTCTTCATG CCACCAACAA CTTGCAAATT 
ATTCCGGACT TCAGCCTCAA GGATTCCCGG GACCAGACTG TGCTGGGCCT GGCATTGTGG 
ACTGGCATGC ATACCATTGC AGCCCAGCTG CTGGGCTCCG GGGCTTCCAT CAATGACACC 
ATGTCAGATG GGCAAACCTT GTTGCACATG GCCATACAGC GGCAGGATAG CAAGAGTGCA 
CTCTTTCTCC TGGAGCATCA GGCAGATATA AATGTCAGCA GGACTCAGGA TGGGGAGACA 
GCCCTTCAGC TGGCCATCAG AAATCAGCTT CCGCTCGTAG TTGATGCCAT ATGCACCCGA 
GGAGCTGACA TGTCCGTTTC AGACGAGAAA GGGAACCCCC CACTGTGGCT TGCGTTGGCA 
AATAATTTGG AAGACATTGC ATCCACTCTG GTCAGACATG GTTGTGATGC CACATGCTGG 
GGTCCAGGAC CTAGCGGTTG CCTCCAAACA CTTCTGCACA GAGCTATTGA TGAAAACAAT 
GAGTCCACTG CCTGCTTTCT TATTCGCAGT GGCTGTGATG TGAATAGTCC CAGACAGCCA 
GGTGCTAATG GAGAAGGAGA GGAAGAAGCC AGGGATGGGC AGACTCCCTT GCATTTGGCA 
GCTTCCTGGG GACTGGAAGA GACTGTGCAG TGTCTTCTGG AATTTGGTGC CAATGTAAAT 
GCACAGGATG CAGAAGGCAG AACACCTGTC CATGTGGCCA TCAGCAACCA ACACAGTGTC 
ATCATTCAGT TGTTGATTTC TCACCCTGAT ATCCACTTGA ATGTACGAGA CAGGCAAGGG 
CTGACCCCAT TTGCCTGTGC CATGACTTAC AAGAACAACA AGGCAGCTGA GGCCATTCTA 
AAACGAGAGT CTGGAGCTGC TGAGCAGGTG GATAACAAGG GCCGGAATTT TCTTCACGTG 
GCAGTTCAGA ACTCTGATAT TGAAAGTGTC CTGTTCTTGA TCAGTGTCCA GGCTAATGTG 
AATTCCAGAG TCCAGGATGC TTCCAAGTTG ACTCCTTTGC ATCTTGCTGT CCAAGCAGGC 
TCAGAGATTA TTGTTCGCAA TTTGCTTCTT GCAGGAGCCA AGGTGAATGA ATTAACCAAG 
CATCGCCAAA CTGCCCTCCA TCTTGCTGCC CAGCAGGACC TGCCTACCAT CTGCTCAGTT 
CTCCTAGAGA ATGGAGTAGA CTTTGCTGCT GTGGATGAGA ATGGAAATAA TGCTCTTCAT 
CTTGCTGTCA TGCATGGTCG ACTCAACAAC ATCCGGGTTC TCCTGACAGA GTGCACAGTG 
GATGCTGAAG CCTTTAATCT GAGAGGCCAG TCACCACTGC ATATTTTGGG ACAGTATGGC 
AAAGAGAATG CAGCAGCCAT CTTTGATCTG TTCTTAGAGT GCATGCCTGA GTATCCTCTA 
GATAAACCAG ATGCAGAAGG AAACACAGTG CTGCTCTTAG CATACATGAA AGGGAATGCC 
AACTTGTGCC GTGCCATCGT CCGATCTGGG GCTCGTCTTG GGGTGAATAA TAACCAAGGT 
GTGAATATTT TCAACTACCA GGTTGCAACC AAGCAACTTC TGTTTAGACT ATTAGACATG 
CTCTCCAAGG AACCTCCATG GTGTGATGGC TCCAACTGCT ACGAGTGCAC TGCCAAGTTT 
GGAGTCACAA CGCGCAAGCA TCACTGTCGG CATTGTGGAC GTCTGCTTTG CCATAAATGT 
TCTACCAAGG AAATTCCTAT TATAAAGTTT GATCTGAACA AGCCTGTGCG AGTCTGCAAC 
ATTTGCTTTG ATGTACTGAC ACTGGGTGGA GTCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015361427.1
CDS	245..3283
Translation	MSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMGAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEVDHNGDLALDLALSRRLESIATTLVSHKADVDMVDKNGWSLLHKGIQRGDLFASTFLIKNGALVNAATLGAQETPLHLVALYSSKKHSTDVMSEMAQIAEALLQAGANPNMQDSKGRTPLHLSITARNEYVFNQLLQCKQLDLELKDHEGSTALWLAVQYITVSSDASVNPFEDLPVVNGTSFDENSFAARLIQRGSNTDAPDTMTGNCLLQRAAGAGNEAAALFLATNGAHVNHKNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEVTSLTSSMDNVYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGASINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVSRTQDGETALQLAIRNQLPLVVDAICTRGADMSVSDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAIDENNESTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPDIHLNVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVQANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPEYPLDKPDAEGNTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSNCYECTAKFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS

Target ORF information:

RefSeq Version	XM_015505941.1
Organism	Marmota marmota marmota(Alpine marmot)
Definition	Marmota marmota marmota ankyrin repeat and FYVE domain containing 1 (Ankfy1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015505941.1

ORF Insert Sequence:

ATGTCCCTGG TGAATGTTAG GAACTGTATT CGCTTCTATC AGACTGCAGA GGAACTGAAT 
GCCAGCACAC TGATGAACTA CTGTGCAGAG ATTATTGCCA GTCATTGGGA TGACTTACGA 
AAGGAGGACT TTAGCAGTAT GGGTGCTCAG TTGTTATACA AAATGATCAA ATCCAAGACA 
GAGTACCCAT TACACAAAGC TATCAAAGTG GAGAGAGAAG ATGTGGTCTT CCTTTATCTG 
ATTGAAATGG ATTCACAGCT CCCTGGGAAG CTAAATGAAG TGGATCATAA TGGAGATCTT 
GCATTAGATC TAGCCCTTTC ACGACGACTA GAGAGTATTG CTACCACGCT GGTTAGTCAT 
AAAGCTGATG TGGACATGGT AGACAAGAAT GGCTGGAGCT TGTTACATAA AGGAATCCAA 
AGAGGAGATC TCTTTGCTTC TACTTTCCTT ATTAAGAATG GGGCCCTTGT TAATGCTGCT 
ACATTGGGTG CCCAAGAGAC ACCATTACAC CTTGTGGCAT TGTACAGTTC AAAGAAACAT 
TCAACAGATG TGATGTCTGA GATGGCACAG ATTGCAGAGG CCCTCCTACA GGCTGGTGCC 
AATCCCAACA TGCAGGATAG CAAGGGCAGG ACTCCTTTAC ACCTGTCCAT CACCGCCAGG 
AATGAATATG TGTTCAATCA GCTGCTGCAG TGTAAACAGT TAGATTTAGA GCTAAAAGAC 
CATGAAGGCA GCACAGCTTT GTGGCTGGCA GTACAGTACA TCACTGTATC TTCGGATGCG 
TCTGTAAACC CCTTTGAAGA CCTCCCTGTG GTAAATGGGA CTTCATTTGA TGAAAACAGC 
TTTGCAGCCA GACTCATTCA GCGTGGCAGC AACACTGATG CACCTGATAC CATGACAGGA 
AATTGTTTAC TGCAGCGGGC AGCTGGAGCA GGAAATGAGG CAGCAGCACT TTTCCTGGCG 
ACCAATGGTG CCCATGTCAA CCACAAAAAC AAGTGGGGAG AAACCCCATT GCACACAGCT 
TGCCGGCACG GCCTGGCCAA CCTCACAGCA GAGCTCCTGC AGCAAGGTGC CAACCCAAAC 
CTGCAGACAG AGGAGGCTCT GCCTTTGCCA AAGGAGGTCA CATCCCTAAC CAGCTCCATG 
GACAATGTGT ATCTGCAGAC GCCGCTTCAC ATGGCAATTG CTTATAATCA CCCAGATGTG 
GTATCTGTCA TCCTGGAGCA GAAAGCCAAT GCTCTTCATG CCACCAACAA CTTGCAAATT 
ATTCCGGACT TCAGCCTCAA GGATTCCCGG GACCAGACTG TGCTGGGCCT GGCATTGTGG 
ACTGGCATGC ATACCATTGC AGCCCAGCTG CTGGGCTCCG GGGCTTCCAT CAATGACACC 
ATGTCAGATG GGCAAACCTT GTTGCACATG GCCATACAGC GGCAGGATAG CAAGAGTGCA 
CTCTTTCTCC TGGAGCATCA GGCAGATATA AATGTCAGCA GGACTCAGGA TGGGGAGACA 
GCCCTTCAGC TGGCCATCAG AAATCAGCTT CCGCTCGTAG TTGATGCCAT ATGCACCCGA 
GGAGCTGACA TGTCCGTTTC AGACGAGAAA GGGAACCCCC CACTGTGGCT TGCGTTGGCA 
AATAATTTGG AAGACATTGC ATCCACTCTG GTCAGACATG GTTGTGATGC CACATGCTGG 
GGTCCAGGAC CTAGCGGTTG CCTCCAAACA CTTCTGCACA GAGCTATTGA TGAAAACAAT 
GAGTCCACTG CCTGCTTTCT TATTCGCAGT GGCTGTGATG TGAATAGTCC CAGACAGCCA 
GGTGCTAATG GAGAAGGAGA GGAAGAAGCC AGGGATGGGC AGACTCCCTT GCATTTGGCA 
GCTTCCTGGG GACTGGAAGA GACTGTGCAG TGTCTTCTGG AATTTGGTGC CAATGTAAAT 
GCACAGGATG CAGAAGGCAG AACACCTGTC CATGTGGCCA TCAGCAACCA ACACAGTGTC 
ATCATTCAGT TGTTGATTTC TCACCCTGAT ATCCACTTGA ATGTACGAGA CAGGCAAGGG 
CTGACCCCAT TTGCCTGTGC CATGACTTAC AAGAACAACA AGGCAGCTGA GGCCATTCTA 
AAACGAGAGT CTGGAGCTGC TGAGCAGGTG GATAACAAGG GCCGGAATTT TCTTCACGTG 
GCAGTTCAGA ACTCTGATAT TGAAAGTGTC CTGTTCTTGA TCAGTGTCCA GGCTAATGTG 
AATTCCAGAG TCCAGGATGC TTCCAAGTTG ACTCCTTTGC ATCTTGCTGT CCAAGCAGGC 
TCAGAGATTA TTGTTCGCAA TTTGCTTCTT GCAGGAGCCA AGGTGAATGA ATTAACCAAG 
CATCGCCAAA CTGCCCTCCA TCTTGCTGCC CAGCAGGACC TGCCTACCAT CTGCTCAGTT 
CTCCTAGAGA ATGGAGTAGA CTTTGCTGCT GTGGATGAGA ATGGAAATAA TGCTCTTCAT 
CTTGCTGTCA TGCATGGTCG ACTCAACAAC ATCCGGGTTC TCCTGACAGA GTGCACAGTG 
GATGCTGAAG CCTTTAATCT GAGAGGCCAG TCACCACTGC ATATTTTGGG ACAGTATGGC 
AAAGAGAATG CAGCAGCCAT CTTTGATCTG TTCTTAGAGT GCATGCCTGA GTATCCTCTA 
GATAAACCAG ATGCAGAAGG AAACACAGTG CTGCTCTTAG CATACATGAA AGGGAATGCC 
AACTTGTGCC GTGCCATCGT CCGATCTGGG GCTCGTCTTG GGGTGAATAA TAACCAAGGT 
GTGAATATTT TCAACTACCA GGTTGCAACC AAGCAACTTC TGTTTAGACT ATTAGACATG 
CTCTCCAAGG AACCTCCATG GTGTGATGGC TCCAACTGCT ACGAGTGCAC TGCCAAGTTT 
GGAGTCACAA CGCGCAAGCA TCACTGTCGG CATTGTGGAC GTCTGCTTTG CCATAAATGT 
TCTACCAAGG AAATTCCTAT TATAAAGTTT GATCTGAACA AGCCTGTGCG AGTCTGCAAC 
ATTTGCTTTG ATGTACTGAC ACTGGGTGGA GTCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Ankfy1 cDNA ORF clone, Marmota marmota marmota(Alpine marmot)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム