LOC103447109 cDNA ORF clone, Malus domestica(apple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103447109
Entrez Gene ID	103447109
Full Name	probable disease resistance protein At4g27220
Gene Type	protein-coding
Organism	Malus domestica(apple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008386285.2	XP_008384507.1	probable disease resistance protein At4g27220

Pathways from BioSystems
Gene Ontology

The following LOC103447109 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103447109 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMa117884	XM_008386285.2 Latest version!	Malus x domestica probable disease resistance protein At4g27220 (LOC103447109), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103447109 ( XM_008386285.2 ) cDNA ORF clone, Malus domestica(apple) -> XP_008384507.1 Malus x domestica probable disease resistance protein At4g27220 (LOC103447109), mRNA.

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CloneID	OMa117884
Clone ID Related Accession (Same CDS sequence)	XM_008386285.2
Accession Version	XM_008386285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Malus domestica(apple)
Product	probable disease resistance protein At4g27220
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024249.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_008386285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Malus domestica Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTG TCAATTTCGT ACTACAAGTT GCTGGGTTGT TATGGACTCC AGTGAAGCGT 
AATGTGGGTT ACTTGGTGCA TTACAAGAGA CACATACAGT CTCTTGAAGT TCTGGTAGAA 
AAGCTTGAGA CCACGCAGAA TGATTACCAG CGAAGCGTGG ATGCGGCGTT AATGAATGGA 
GACGAAGTTA AGTCCGAGGT TCAAAAATGG CTCAAGGATG CTGACAAGGC CATAATCGAT 
GCGAAAAGAC TGAATAATGA AGCCGGAGAA AACAAAACAT GCCTTGGAGG CTGCTGCCCG 
AATTTGAAAT GGCGTTACAC TTTAAGCAAG AGAGCAGTTA ATGAGACAGA AGAATTGAAC 
AAGCTTAAAG AAGAGAAAAG GTTTGAAACA GTTACGCTTC AAGTTCGCCG GCCCGTTGAG 
TTTGAGTCCA CCATGTCAAC AGGAGATTTC GAAGCATTTG AAGCAACAAA GCAAGCCATG 
GATGGGGTCA TGAAGGCGCT GAAAGACGAT AACGTCACTG TCATTGGGGT TCATGGAATG 
GGAGGCATAG GCAAGACAAC CATGGTGAAA CATGTTGGTT TGCAAGCGTG TAAAGACAAA 
CTCTTTGATC ATGTGATTAT GGCTGTCATT TCCCAAAACC CGAACCTGGT TAAGATTCAA 
CAACAGCTTG CAGAAATGCT GGCCTTGAAT TTGAATGAGC AGACAGAAAT AGCCAGAGCA 
GCTAGATTGA AGGAGAGAAT AATGAGAGGA AAAAAGATCC TTATAATTTT CGATGATATT 
TGGAGGGCAA TAGATTTGTC TCTCATAGGG ATCCCCGACG ACTACGAGCT CCAAAACTGC 
AATTCCAAGG TCCTTCTCAC CACAAGGTTA CAGAATGTAT GTCATGCCAT GAAGAGCCAA 
GAAAAGATCC ATCTCCATAT CCTATCAGAA GAAGATTCTT GGGCCTTGTT TGTGAAAAAG 
GCAAACAGGC CTTTTGAATC AACCAATTTC TATGACATAG CAAGGAAGGT AGCTGGAGAA 
TGCCATGGTC TCCCAATTGC TTTGATTGCA GTTGCAAGAG CACTCGGAGA TAAGGACTTG 
GAAGAATGGA GAGAAGCAGC TCGACGACTA GAGATGTCCC AAATTACCAA CCTTGATGAC 
GATGGAGATG TGTTCAGATG TATAAAGCTG AGCTACGATT ACTTGAATGG CAATGACGCC 
AAGTCATTTT TCTTACTTTG CTGCCTATTC CCGGAGGACT CTGATATCCA AATCGAAGAC 
TTGCTGAAGT ATGGCATCGG GAAAGGATTG TTTCGAGATG CCAATTCAAT GCAACAAGCC 
AGAAGCACAG CACATTTGGT GGCCAAGTAC CTTAAAGCTT GTAGCTTGGT TTTGGATGGT 
GAACAAGACG GATGTGTAAG AATGCATGAT GTGATTCGGG ACATGGCAAT ACTAATTGCA 
TCATCTGGGG ATGGTGGCCA AGGATTCTTA GTAAAAGCTG GCTGCGACTT AAAGGATTGG 
CCAAAGAATT CACTCGAAGA TTACTCCGCA ATCTCCCTAA TGGGGAATGA AATCTGCAAT 
CTTCCCGACG AGTTGGTATG TCCAAAACTC GAGATTTTAT TACTACAATC CAATTTTGAA 
GTAGAGGAGA TCCCAGAAGA TTTTTTCCAA AGCCCAAATG CATTAAGGGT GTTCGACATA 
AGTAGTACCG AAATTATTTC ATTACCCTCA TCATTTAATC TCTTAACCAA ACTCCAAACG 
CTGCATCTAG ATCGTTGTCA GTCAATAACG GATGTATCAA TTCTCGGAGA ATTGAAAAAA 
CTGGAGGTTC TTAGTATGAG AGAATCTGGT ATTGAGGCAT TGCCAGAAGA AATTGGACAT 
TTGACCAATT TAAGGATATT GGATCTCACT ATGAGTACCT ACATTGCAGC AATTCCATCC 
AATGTGATAT CGCGGCTGTC CAAATTAGAA GAACTATACA TGCAACTGAG TTTTGCTGAT 
TGGGGCAAGA CAGTTGAAGG AGAAGATGAC AAAACTAATG CTAGTTTTGA TGAGCTAATT 
TGTTTGCCTT ACTTAAACAG TTTGAAGGTT GACATATCTG ATGCAGAATG CCTTCCGGAA 
AATGTAGGGT TCTTTCGAAA TTGGGTAAAT TTCGATATAT GTATCAGCAG AGACCCAGTT 
CATCGGTCCA TGAATGTGCA CTTATCAAAG AATATTATTA CTCCATATTC AAGAGCCCTG 
TTTCTGGACA CAACAATCAA TACACTGCCG GATTGGTTTA ACAGCACAGT GACGGAGAGA 
GTGGAGAAAA TAATCTACTT GGAGCCTAGA AGTTTAACTG ACATTCTTGT GGAGTACAAT 
CATGGGAAGT TGCATGGACT CAAGTTTTTG CATCTCCAAG AGTGCCGTGA GATGGTAACT 
TTGATGAACA CAGAAACTAG GGTTCCAAAT AAGCCTGTGC TCGAGAGCTT GGAGGAGTTG 
CATGTCTTCT GCTGGGACCG CTTGCAACAG TTATGTGTTG GTGAATTGCT GCGGGGGTCG 
CTTGGAAAAC TCAAGTTCTT GGAGGTGGAG CAGTGTGAAG GTGTGGCGGA TGCGCTTGTT 
CCATCTAATC TATTGCAGAG ACTACAAAAT CTTGAAGTGC TCATTGTATG TGCCACTGGT 
ATGGTTTATA TATTCGAATC TCAAGGGCTT GAACAAGGAG AAACGGTCTT GACAAAATTG 
AGAGAGATGA AACTGGAGAA TCTACCGGAA CTGACAAACA TATTGAACGG TCCTGCTCAG 
CCTGCAATGT TCCATAACCT TAAAACTCTG GCGATTTCCA AGTGCAAGAA ATTGAAAACT 
CTCTTCACAT TCGACGTAGC TCAATGTCTG CTGCAATTGG AAGAGCTTTG GGTGGAGGAA 
TGCCATAGCT TGGACAAACT TTTCGGCCTA ARCGAGGTAA TAGTTGCAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008384507.1
CDS	102..3035
Translation	MEIVNFVLQVAGLLWTPVKRNVGYLVHYKRHIQSLEVLVEKLETTQNDYQRSVDAALMNGDEVKSEVQKWLKDADKAIIDAKRLNNEAGENKTCLGGCCPNLKWRYTLSKRAVNETEELNKLKEEKRFETVTLQVRRPVEFESTMSTGDFEAFEATKQAMDGVMKALKDDNVTVIGVHGMGGIGKTTMVKHVGLQACKDKLFDHVIMAVISQNPNLVKIQQQLAEMLALNLNEQTEIARAARLKERIMRGKKILIIFDDIWRAIDLSLIGIPDDYELQNCNSKVLLTTRLQNVCHAMKSQEKIHLHILSEEDSWALFVKKANRPFESTNFYDIARKVAGECHGLPIALIAVARALGDKDLEEWREAARRLEMSQITNLDDDGDVFRCIKLSYDYLNGNDAKSFFLLCCLFPEDSDIQIEDLLKYGIGKGLFRDANSMQQARSTAHLVAKYLKACSLVLDGEQDGCVRMHDVIRDMAILIASSGDGGQGFLVKAGCDLKDWPKNSLEDYSAISLMGNEICNLPDELVCPKLEILLLQSNFEVEEIPEDFFQSPNALRVFDISSTEIISLPSSFNLLTKLQTLHLDRCQSITDVSILGELKKLEVLSMRESGIEALPEEIGHLTNLRILDLTMSTYIAAIPSNVISRLSKLEELYMQLSFADWGKTVEGEDDKTNASFDELICLPYLNSLKVDISDAECLPENVGFFRNWVNFDICISRDPVHRSMNVHLSKNIITPYSRALFLDTTINTLPDWFNSTVTERVEKIIYLEPRSLTDILVEYNHGKLHGLKFLHLQECREMVTLMNTETRVPNKPVLESLEELHVFCWDRLQQLCVGELLRGSLGKLKFLEVEQCEGVADALVPSNLLQRLQNLEVLIVCATGMVYIFESQGLEQGETVLTKLREMKLENLPELTNILNGPAQPAMFHNLKTLAISKCKKLKTLFTFDVAQCLLQLEELWVEECHSLDKLFGLnullEVIVAG

Target ORF information:

RefSeq Version	XM_008386285.2
Organism	Malus domestica(apple)
Definition	Malus x domestica probable disease resistance protein At4g27220 (LOC103447109), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008386285.2

ORF Insert Sequence:

ATGGAAATTG TCAATTTCGT ACTACAAGTT GCTGGGTTGT TATGGACTCC AGTGAAGCGT 
AATGTGGGTT ACTTGGTGCA TTACAAGAGA CACATACAGT CTCTTGAAGT TCTGGTAGAA 
AAGCTTGAGA CCACGCAGAA TGATTACCAG CGAAGCGTGG ATGCGGCGTT AATGAATGGA 
GACGAAGTTA AGTCCGAGGT TCAAAAATGG CTCAAGGATG CTGACAAGGC CATAATCGAT 
GCGAAAAGAC TGAATAATGA AGCCGGAGAA AACAAAACAT GCCTTGGAGG CTGCTGCCCG 
AATTTGAAAT GGCGTTACAC TTTAAGCAAG AGAGCAGTTA ATGAGACAGA AGAATTGAAC 
AAGCTTAAAG AAGAGAAAAG GTTTGAAACA GTTACGCTTC AAGTTCGCCG GCCCGTTGAG 
TTTGAGTCCA CCATGTCAAC AGGAGATTTC GAAGCATTTG AAGCAACAAA GCAAGCCATG 
GATGGGGTCA TGAAGGCGCT GAAAGACGAT AACGTCACTG TCATTGGGGT TCATGGAATG 
GGAGGCATAG GCAAGACAAC CATGGTGAAA CATGTTGGTT TGCAAGCGTG TAAAGACAAA 
CTCTTTGATC ATGTGATTAT GGCTGTCATT TCCCAAAACC CGAACCTGGT TAAGATTCAA 
CAACAGCTTG CAGAAATGCT GGCCTTGAAT TTGAATGAGC AGACAGAAAT AGCCAGAGCA 
GCTAGATTGA AGGAGAGAAT AATGAGAGGA AAAAAGATCC TTATAATTTT CGATGATATT 
TGGAGGGCAA TAGATTTGTC TCTCATAGGG ATCCCCGACG ACTACGAGCT CCAAAACTGC 
AATTCCAAGG TCCTTCTCAC CACAAGGTTA CAGAATGTAT GTCATGCCAT GAAGAGCCAA 
GAAAAGATCC ATCTCCATAT CCTATCAGAA GAAGATTCTT GGGCCTTGTT TGTGAAAAAG 
GCAAACAGGC CTTTTGAATC AACCAATTTC TATGACATAG CAAGGAAGGT AGCTGGAGAA 
TGCCATGGTC TCCCAATTGC TTTGATTGCA GTTGCAAGAG CACTCGGAGA TAAGGACTTG 
GAAGAATGGA GAGAAGCAGC TCGACGACTA GAGATGTCCC AAATTACCAA CCTTGATGAC 
GATGGAGATG TGTTCAGATG TATAAAGCTG AGCTACGATT ACTTGAATGG CAATGACGCC 
AAGTCATTTT TCTTACTTTG CTGCCTATTC CCGGAGGACT CTGATATCCA AATCGAAGAC 
TTGCTGAAGT ATGGCATCGG GAAAGGATTG TTTCGAGATG CCAATTCAAT GCAACAAGCC 
AGAAGCACAG CACATTTGGT GGCCAAGTAC CTTAAAGCTT GTAGCTTGGT TTTGGATGGT 
GAACAAGACG GATGTGTAAG AATGCATGAT GTGATTCGGG ACATGGCAAT ACTAATTGCA 
TCATCTGGGG ATGGTGGCCA AGGATTCTTA GTAAAAGCTG GCTGCGACTT AAAGGATTGG 
CCAAAGAATT CACTCGAAGA TTACTCCGCA ATCTCCCTAA TGGGGAATGA AATCTGCAAT 
CTTCCCGACG AGTTGGTATG TCCAAAACTC GAGATTTTAT TACTACAATC CAATTTTGAA 
GTAGAGGAGA TCCCAGAAGA TTTTTTCCAA AGCCCAAATG CATTAAGGGT GTTCGACATA 
AGTAGTACCG AAATTATTTC ATTACCCTCA TCATTTAATC TCTTAACCAA ACTCCAAACG 
CTGCATCTAG ATCGTTGTCA GTCAATAACG GATGTATCAA TTCTCGGAGA ATTGAAAAAA 
CTGGAGGTTC TTAGTATGAG AGAATCTGGT ATTGAGGCAT TGCCAGAAGA AATTGGACAT 
TTGACCAATT TAAGGATATT GGATCTCACT ATGAGTACCT ACATTGCAGC AATTCCATCC 
AATGTGATAT CGCGGCTGTC CAAATTAGAA GAACTATACA TGCAACTGAG TTTTGCTGAT 
TGGGGCAAGA CAGTTGAAGG AGAAGATGAC AAAACTAATG CTAGTTTTGA TGAGCTAATT 
TGTTTGCCTT ACTTAAACAG TTTGAAGGTT GACATATCTG ATGCAGAATG CCTTCCGGAA 
AATGTAGGGT TCTTTCGAAA TTGGGTAAAT TTCGATATAT GTATCAGCAG AGACCCAGTT 
CATCGGTCCA TGAATGTGCA CTTATCAAAG AATATTATTA CTCCATATTC AAGAGCCCTG 
TTTCTGGACA CAACAATCAA TACACTGCCG GATTGGTTTA ACAGCACAGT GACGGAGAGA 
GTGGAGAAAA TAATCTACTT GGAGCCTAGA AGTTTAACTG ACATTCTTGT GGAGTACAAT 
CATGGGAAGT TGCATGGACT CAAGTTTTTG CATCTCCAAG AGTGCCGTGA GATGGTAACT 
TTGATGAACA CAGAAACTAG GGTTCCAAAT AAGCCTGTGC TCGAGAGCTT GGAGGAGTTG 
CATGTCTTCT GCTGGGACCG CTTGCAACAG TTATGTGTTG GTGAATTGCT GCGGGGGTCG 
CTTGGAAAAC TCAAGTTCTT GGAGGTGGAG CAGTGTGAAG GTGTGGCGGA TGCGCTTGTT 
CCATCTAATC TATTGCAGAG ACTACAAAAT CTTGAAGTGC TCATTGTATG TGCCACTGGT 
ATGGTTTATA TATTCGAATC TCAAGGGCTT GAACAAGGAG AAACGGTCTT GACAAAATTG 
AGAGAGATGA AACTGGAGAA TCTACCGGAA CTGACAAACA TATTGAACGG TCCTGCTCAG 
CCTGCAATGT TCCATAACCT TAAAACTCTG GCGATTTCCA AGTGCAAGAA ATTGAAAACT 
CTCTTCACAT TCGACGTAGC TCAATGTCTG CTGCAATTGG AAGAGCTTTG GGTGGAGGAA 
TGCCATAGCT TGGACAAACT TTTCGGCCTA ARCGAGGTAA TAGTTGCAGG ATGA