LOC103405483 cDNA ORF clone, Malus domestica(apple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103405483
Entrez Gene ID	103405483
Full Name	regulator of telomere elongation helicase 1 homolog
Gene Type	protein-coding
Organism	Malus domestica(apple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017324051.1	XP_017179540.1	regulator of telomere elongation helicase 1 homolog
XM_029097587.1	XP_028953420.1	LOW QUALITY PROTEIN: regulator of telomere elongation helicase 1 homolog

Pathways from BioSystems
Gene Ontology

The following LOC103405483 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103405483 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMa128622	XM_017324051.1 Latest version!	Malus x domestica regulator of telomere elongation helicase 1 homolog (LOC103405483), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OMa450947	XM_029097587.1 Latest version!	Malus domestica regulator of telomere elongation helicase 1 homolog (LOC103405483), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103405483 ( XM_017324051.1 ) cDNA ORF clone, Malus domestica(apple) -> XP_017179540.1 Malus x domestica regulator of telomere elongation helicase 1 homolog (LOC103405483), mRNA.

Price & Availability↑

CloneID	OMa128622
Clone ID Related Accession (Same CDS sequence)	XM_017324051.1
Accession Version	XM_017324051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Malus domestica(apple)
Product	regulator of telomere elongation helicase 1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024255.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Malus domestica Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCCGACGT ACAACATCAG AGGCATCGAT GTCGATTTCC CATTTGAGGC GTACGATTGC 
CAGCTCGTTT ACATGGAGAA AGTCATTCAA TCTCTCCAAG AAAAAAGTAA TGCACTGCTG 
GAGAGTCCCA CTGGAACTGG GAAGACCCTT TGTCTTCTGT GCGCCACATT GGCCTGGAGG 
AAGAGTTTGG GCCCTTTCTC AACTGGTCCT AATGTCAAGA TGAGTGTGAT CACAGGAGAT 
GCATCAGGTG GTCCCTCGTC ACAGTCTGTG ACAAAAGGTT TTCCTACCAT AGTATATACG 
TCCCGCACTC ACAGCCAGAT CCGGCAAGTA ATTCAAGAGT TGAAAAGAAG CGCTTACAGG 
CCAAAAATGG TAGTGTTGGG ATCTCGAGAG CAATTATGCA TTGATGAGGA AGTGAGTTTA 
CTTCGCGGAA GAACACAGAC AAAGGCTTGC CATGTCCTCT GCAGAAAGCG CACAAAGGAT 
ACAAAGCGTC GTTGTTCTCA TTACTCTCGT GGTTCTGGTT TCTTGAAGAG TAATCCTCAT 
CTTGGAGATG AACCTGTTGA TATCGAGGAT TTGGTCAATA CTGGAAAAAA ATTGGGGCCG 
TGTCCTTATT ATTTATCGAG GGAGCTCCAT AAGGTTGTTG ATATATTATT TGCACCTTAT 
AACTATCTCA TTGATCCTGC ATTTAGAAAA TCGCTACAGG TTGATTGGAA AAACAGTATT 
CTGATATTTG AYGAAGCTCA CAACCTGGAA AGCATATGTG CAGATGCTGC TTCCTTTGAC 
TTGCCCTCTT GGCTTCTTAC AACTTGCATT TCTGAAGCAA AAAATTGTAT TGACCTTTCT 
TCAAAAAGAA GGGAAGACTC AACTGATAAA TCACAGAATC CAGATGACTT TGCTATCCTC 
AGAGCGCTAC TATTGAAGCT TGAGAAGCGT ATTTCTGAAG TACCTATCGA ATCCAAGGAA 
TTGGGATACA CAAAGCCTGG GCCTTATATC TATGAACTCC TGGCGGATTT AAACATCTCG 
CATGATACTG TCCCTAAGCT CATTGCAATA CTTGAGGACG CTACTGTACT TATTGAGGAA 
GATAACCAGG ACAAGGCGAA TCGAAATGCC TGCCGGCTGG AAAGCATTAG TGACATGCTT 
AATATAATTT TCAGAGATAA AGACAATAAT CATGCGAAAT CCTATCGTGT TCATGTACAA 
GAAGTTGAAG CCAGTGCAAT AGATGGTTTA AAGGGGAAGG CTTCTAGGAC ACTTAGCTGG 
TGGTGTTTTC ATCCTGGAAT TGCAATGGAA GAATTTGCCA GGATAGGTGT GGGCTCTATT 
ATACTGACAT CTGGCACATT ATCTCCCTTG GATTCGTTTG CACAGGAACT GAAACTAGAG 
TTTCCTGTGC GGCTGGAAAA TCCTCATGTT ATTACCCCGA ATCAGATATG GGCTGGAGTT 
GTACCAGCTG GCCCTTCTGG ATTCTCTTTC AACTCCTCTT ACAGAAATCG TGATTCTACG 
GAGTACAAGC AGGACCTTGG AAATGCTATT GTCAATTTTG CTCGAATTGT TCCCAATGGA 
TTGCTAGTAT TCTTTTCGTC GTACTACATT CTGGACCAAT GCATTGCCTG CTGGAAGAAA 
ATGAGCCATG CCAATTCAAC TACAACATGG GAAAGAATCT CTAAACATAA GAAACCTGTT 
GTGGAACCTA GACAATCTTC GTTGTTTCTT TTGTCAATTG AGGATTATAT GGCAAAGCTG 
AAGGACACCT CAGCTTCTGG TGCAGTATTT TTTGCTGTTT GCCGTGGTAA AGTAAGTGAA 
GGACTAGATT TTGCTGATCA TGCTGGAAGA GCTGTAGTCA TTACTGGTCT ACCATTTGCC 
ACAAGGAATG ATCCTAAGGT TCGACTGAAA CGTGAATACT TGGATCAGCA GGCATATTCC 
CAAAGGCAAG CATGTAAGTT GAAGGTTCTA ACTGGAGAAG ACTGGTACAA TCAACAAGCA 
TTACGAGCTG TGAATCAGGC TGTTGGGCGC GTAATCCGTC ATCGTCATGA TTATGGAGCA 
ATTATTTTTT GTGATGAAAG CAAATTTGAG CTGAACTCAA TTGTCATTCC ATTTCCAGTG 
CTGCTGTTTA AAGCATCTTT TGTGGTGCGA CAACCTTTTA ATGCTGTAGT TAATTTGATT 
ACCTTTATTC ATTTTGTAGG AACCCCAGGG AAAATATATG AGTTGAAAAC TGAGGAACCA 
CCAGAAATAT TTTACTTGGA CCAGTTTCTA CCTAAGACAA CTGCAGTTGG TCAAGACCCC 
GCTGTAAAAT CGCCTTCTTT CCTACTTAAA GCTAGAAGGG ACAGAATATC AAGCCTGTTA 
GGCGAAGTCC TTCCTGCCAA CCGCTCATCT CTTACTTGCT GTAAGGATCA GTCTGTGTCA 
TTAAAGTRTT CAAATGATCT ATTTCAAAGT GAGAAAAAGA TGCGTATGTG TGATAGGGGA 
ACTATGCAAT GTCAAGAAAG TAAGGTTCTT GGGTTTCCTG GGAAAAATGA GAAACGAAGT 
GAACAACAAC TGATAGCACC TTGTTCCACA AAGAAGTATA AATTAGAGCA TGGTGCAGAA 
CAACATGACA GTTCTAAGCA TTCATCTCTT GCAAAAGATG CTCACTCATA TGGTCTTTAC 
TCCACTAATA ATTCAATGAG AAGTGAGAGT TCACATAGAT CTGACAGTGG AAGTGCACAA 
AATGCTCAAG TTGGTTCAGC TCTGCTGTGC AAAGATACGA GGATCATACA ACAGGGAGAT 
GCTGAGTTTC TGAGCAAGAA AAAAACGAGT GCAATATCCA GCCCTGTGCC TTCTGGTGAT 
GGGGAAACCA GAGGATCTGA GTTTCTAGTT CAGGTTGAGG AAAAACTTAG TGCTTTGGAA 
TATAAAGAAT TTGTAAATTT GATGAAGGCA CTTAAGTCCA AAGCTATGAA GATAAATGAG 
GTTTTGCAAT CCATTGCGGG CTTGTTTTCC GGAAGAGAGA GGCTTCCTCT TCTTAAAAGA 
TTTAAGGACT TTATCCCTGC AAAGTATCAC TCTATGTATG AGAACTATTT TCAAACAAAT 
GACGGATCTC TTGATATCCA AGGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017179540.1
CDS	101..3190
Translation	MPTYNIRGIDVDFPFEAYDCQLVYMEKVIQSLQEKSNALLESPTGTGKTLCLLCATLAWRKSLGPFSTGPNVKMSVITGDASGGPSSQSVTKGFPTIVYTSRTHSQIRQVIQELKRSAYRPKMVVLGSREQLCIDEEVSLLRGRTQTKACHVLCRKRTKDTKRRCSHYSRGSGFLKSNPHLGDEPVDIEDLVNTGKKLGPCPYYLSRELHKVVDILFAPYNYLIDPAFRKSLQVDWKNSILIFnullEAHNLESICADAASFDLPSWLLTTCISEAKNCIDLSSKRREDSTDKSQNPDDFAILRALLLKLEKRISEVPIESKELGYTKPGPYIYELLADLNISHDTVPKLIAILEDATVLIEEDNQDKANRNACRLESISDMLNIIFRDKDNNHAKSYRVHVQEVEASAIDGLKGKASRTLSWWCFHPGIAMEEFARIGVGSIILTSGTLSPLDSFAQELKLEFPVRLENPHVITPNQIWAGVVPAGPSGFSFNSSYRNRDSTEYKQDLGNAIVNFARIVPNGLLVFFSSYYILDQCIACWKKMSHANSTTTWERISKHKKPVVEPRQSSLFLLSIEDYMAKLKDTSASGAVFFAVCRGKVSEGLDFADHAGRAVVITGLPFATRNDPKVRLKREYLDQQAYSQRQACKLKVLTGEDWYNQQALRAVNQAVGRVIRHRHDYGAIIFCDESKFELNSIVIPFPVLLFKASFVVRQPFNAVVNLITFIHFVGTPGKIYELKTEEPPEIFYLDQFLPKTTAVGQDPAVKSPSFLLKARRDRISSLLGEVLPANRSSLTCCKDQSVSLKnullSNDLFQSEKKMRMCDRGTMQCQESKVLGFPGKNEKRSEQQLIAPCSTKKYKLEHGAEQHDSSKHSSLAKDAHSYGLYSTNNSMRSESSHRSDSGSAQNAQVGSALLCKDTRIIQQGDAEFLSKKKTSAISSPVPSGDGETRGSEFLVQVEEKLSALEYKEFVNLMKALKSKAMKINEVLQSIAGLFSGRERLPLLKRFKDFIPAKYHSMYENYFQTNDGSLDIQGK

Target ORF information:

RefSeq Version	XM_017324051.1
Organism	Malus domestica(apple)
Definition	Malus x domestica regulator of telomere elongation helicase 1 homolog (LOC103405483), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017324051.1

ORF Insert Sequence:

ATGCCGACGT ACAACATCAG AGGCATCGAT GTCGATTTCC CATTTGAGGC GTACGATTGC 
CAGCTCGTTT ACATGGAGAA AGTCATTCAA TCTCTCCAAG AAAAAAGTAA TGCACTGCTG 
GAGAGTCCCA CTGGAACTGG GAAGACCCTT TGTCTTCTGT GCGCCACATT GGCCTGGAGG 
AAGAGTTTGG GCCCTTTCTC AACTGGTCCT AATGTCAAGA TGAGTGTGAT CACAGGAGAT 
GCATCAGGTG GTCCCTCGTC ACAGTCTGTG ACAAAAGGTT TTCCTACCAT AGTATATACG 
TCCCGCACTC ACAGCCAGAT CCGGCAAGTA ATTCAAGAGT TGAAAAGAAG CGCTTACAGG 
CCAAAAATGG TAGTGTTGGG ATCTCGAGAG CAATTATGCA TTGATGAGGA AGTGAGTTTA 
CTTCGCGGAA GAACACAGAC AAAGGCTTGC CATGTCCTCT GCAGAAAGCG CACAAAGGAT 
ACAAAGCGTC GTTGTTCTCA TTACTCTCGT GGTTCTGGTT TCTTGAAGAG TAATCCTCAT 
CTTGGAGATG AACCTGTTGA TATCGAGGAT TTGGTCAATA CTGGAAAAAA ATTGGGGCCG 
TGTCCTTATT ATTTATCGAG GGAGCTCCAT AAGGTTGTTG ATATATTATT TGCACCTTAT 
AACTATCTCA TTGATCCTGC ATTTAGAAAA TCGCTACAGG TTGATTGGAA AAACAGTATT 
CTGATATTTG AYGAAGCTCA CAACCTGGAA AGCATATGTG CAGATGCTGC TTCCTTTGAC 
TTGCCCTCTT GGCTTCTTAC AACTTGCATT TCTGAAGCAA AAAATTGTAT TGACCTTTCT 
TCAAAAAGAA GGGAAGACTC AACTGATAAA TCACAGAATC CAGATGACTT TGCTATCCTC 
AGAGCGCTAC TATTGAAGCT TGAGAAGCGT ATTTCTGAAG TACCTATCGA ATCCAAGGAA 
TTGGGATACA CAAAGCCTGG GCCTTATATC TATGAACTCC TGGCGGATTT AAACATCTCG 
CATGATACTG TCCCTAAGCT CATTGCAATA CTTGAGGACG CTACTGTACT TATTGAGGAA 
GATAACCAGG ACAAGGCGAA TCGAAATGCC TGCCGGCTGG AAAGCATTAG TGACATGCTT 
AATATAATTT TCAGAGATAA AGACAATAAT CATGCGAAAT CCTATCGTGT TCATGTACAA 
GAAGTTGAAG CCAGTGCAAT AGATGGTTTA AAGGGGAAGG CTTCTAGGAC ACTTAGCTGG 
TGGTGTTTTC ATCCTGGAAT TGCAATGGAA GAATTTGCCA GGATAGGTGT GGGCTCTATT 
ATACTGACAT CTGGCACATT ATCTCCCTTG GATTCGTTTG CACAGGAACT GAAACTAGAG 
TTTCCTGTGC GGCTGGAAAA TCCTCATGTT ATTACCCCGA ATCAGATATG GGCTGGAGTT 
GTACCAGCTG GCCCTTCTGG ATTCTCTTTC AACTCCTCTT ACAGAAATCG TGATTCTACG 
GAGTACAAGC AGGACCTTGG AAATGCTATT GTCAATTTTG CTCGAATTGT TCCCAATGGA 
TTGCTAGTAT TCTTTTCGTC GTACTACATT CTGGACCAAT GCATTGCCTG CTGGAAGAAA 
ATGAGCCATG CCAATTCAAC TACAACATGG GAAAGAATCT CTAAACATAA GAAACCTGTT 
GTGGAACCTA GACAATCTTC GTTGTTTCTT TTGTCAATTG AGGATTATAT GGCAAAGCTG 
AAGGACACCT CAGCTTCTGG TGCAGTATTT TTTGCTGTTT GCCGTGGTAA AGTAAGTGAA 
GGACTAGATT TTGCTGATCA TGCTGGAAGA GCTGTAGTCA TTACTGGTCT ACCATTTGCC 
ACAAGGAATG ATCCTAAGGT TCGACTGAAA CGTGAATACT TGGATCAGCA GGCATATTCC 
CAAAGGCAAG CATGTAAGTT GAAGGTTCTA ACTGGAGAAG ACTGGTACAA TCAACAAGCA 
TTACGAGCTG TGAATCAGGC TGTTGGGCGC GTAATCCGTC ATCGTCATGA TTATGGAGCA 
ATTATTTTTT GTGATGAAAG CAAATTTGAG CTGAACTCAA TTGTCATTCC ATTTCCAGTG 
CTGCTGTTTA AAGCATCTTT TGTGGTGCGA CAACCTTTTA ATGCTGTAGT TAATTTGATT 
ACCTTTATTC ATTTTGTAGG AACCCCAGGG AAAATATATG AGTTGAAAAC TGAGGAACCA 
CCAGAAATAT TTTACTTGGA CCAGTTTCTA CCTAAGACAA CTGCAGTTGG TCAAGACCCC 
GCTGTAAAAT CGCCTTCTTT CCTACTTAAA GCTAGAAGGG ACAGAATATC AAGCCTGTTA 
GGCGAAGTCC TTCCTGCCAA CCGCTCATCT CTTACTTGCT GTAAGGATCA GTCTGTGTCA 
TTAAAGTRTT CAAATGATCT ATTTCAAAGT GAGAAAAAGA TGCGTATGTG TGATAGGGGA 
ACTATGCAAT GTCAAGAAAG TAAGGTTCTT GGGTTTCCTG GGAAAAATGA GAAACGAAGT 
GAACAACAAC TGATAGCACC TTGTTCCACA AAGAAGTATA AATTAGAGCA TGGTGCAGAA 
CAACATGACA GTTCTAAGCA TTCATCTCTT GCAAAAGATG CTCACTCATA TGGTCTTTAC 
TCCACTAATA ATTCAATGAG AAGTGAGAGT TCACATAGAT CTGACAGTGG AAGTGCACAA 
AATGCTCAAG TTGGTTCAGC TCTGCTGTGC AAAGATACGA GGATCATACA ACAGGGAGAT 
GCTGAGTTTC TGAGCAAGAA AAAAACGAGT GCAATATCCA GCCCTGTGCC TTCTGGTGAT 
GGGGAAACCA GAGGATCTGA GTTTCTAGTT CAGGTTGAGG AAAAACTTAG TGCTTTGGAA 
TATAAAGAAT TTGTAAATTT GATGAAGGCA CTTAAGTCCA AAGCTATGAA GATAAATGAG 
GTTTTGCAAT CCATTGCGGG CTTGTTTTCC GGAAGAGAGA GGCTTCCTCT TCTTAAAAGA 
TTTAAGGACT TTATCCCTGC AAAGTATCAC TCTATGTATG AGAACTATTT TCAAACAAAT 
GACGGATCTC TTGATATCCA AGGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103405483 ( XM_029097587.1 ) cDNA ORF clone, Malus domestica(apple) -> XP_028953420.1 Malus domestica regulator of telomere elongation helicase 1 homolog (LOC103405483), mRNA.

Price & Availability↑

CloneID	OMa450947
Clone ID Related Accession (Same CDS sequence)	XM_029097587.1
Accession Version	XM_029097587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-02
Organism	Malus domestica(apple)
Product	LOW QUALITY PROTEIN: regulator of telomere elongation helicase 1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Malus domestica Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCGACGT ACAACATCAG AGGCATCGAT GTCGATTTCC CATTTGAGGC GTACGATTGC 
CAGCTCGTTT ACATGGAGAA AGTCATTCAA TCTCTCCAAG AAAAAAGTAA TGCACTGCTG 
GAGAGTCCCA CTGGAACTGG GAAGACCCTT TGTCTTCTGT GCGCCACATT GGCCTGGAGG 
AAGAGTTTGG GCCCTTTCTC AACTGGTCCT AATGTCAAGA TGAGTGTGAT CACAGGAGAT 
GCATCAGGTG GTCCCTCGTC ACAGTCTGTG ACAAAAGGTT TTCCTACCAT AGTATATACG 
TCCCGCACTC ACAGCCAGAT CCGGCAAGTA ATTCAAGAGT TGAAAAGAAG CGCTTACAGG 
CCAAAAATGG TAGTGTTGGG ATCTCGAGAG CAATTATGCA TTGATGAGGA AGTGAGTTTA 
CTTCGCGGAA GAACACAGAC AAAGGCTTGC CATGTCCTCT GCAGAAAGCG CACAAAGGAT 
ACAAAGCGTC GTTGTTCTCA TTACTCTCGT GGTTCTGGTT TCTTGAAGAG TAATCCTCAT 
CTTGGAGATG AACCTGTTGA TATCGAGGAT TTGGTCAATA CTGGAAAAAA ATTGGGGCCG 
TGTCCTTATT ATTTATCGAG GGAGCTCCAT AAGGTTGTTG ATATATTATT TGCACCTTAT 
AACTATCTCA TTGATCCTGC ATTTAGAAAA TCGCTACAGG TTGATTGGAA AAACAGTATT 
CTGATATTTG ACGAAGCTCA CAACCTGGAA AGCATATGTG CAGATGCTGC TTCCTTTGAC 
TTGCCCTCTT GGCTTCTTAC AACTTGCATT TCTGAAGCAA AAAATTGTAT TGACCTTTCT 
TCAAAAAGAA GGGAAGACTC AACTGATAAA TCACAGAATC CAGATGACTT TGCTATCCTC 
AGAGCGCTAC TATTGAAGCT TGAGAAGCGT ATTTCTGAAG TACCTATCGA ATCCAAGGAA 
TTGGGATACA CAAAGCCTGG GCCTTATATC TATGAACTCC TGGCGGATTT AAACATCTCG 
CATGATACTG TCCCTAAGCT CATTGCAATA CTTGAGGACG CTACTGTACT TATTGAGGAA 
GATAACCAGG ACAAGGCGAA TCGAAATGCC TGCCGGCTGG AAAGCATTAG TGACATGCTT 
AATATAATTT TCAGAGATAA AGACAATAAT CATGCGAAAT CCTATCGTGT TCATGTACAA 
GAAGTTGAAG CCAGTGCAAT AGATGGTTTA AAGGGGAAGG CTTCTAGGAC ACTTAGCTGG 
TGGTGTTTTC ATCCTGGAAT TGCAATGGAA GAATTTGCCA GGATAGGTGT GGGCTCTATT 
ATACTGACAT CTGGCACATT ATCTCCCTTG GATTCGTTTG CACAGGAACT GAAACTAGAG 
TTTCCTGTGC GGCTGGAAAA TCCTCATGTT ATTACCCCGA ATCAGATATG GGCTGGAGTT 
GTACCAGCTG GCCCTTCTGG ATTCTCTTTC AACTCCTCTT ACAGAAATCG TGATTCTACG 
GAGTACAAGC AGGACCTTGG AAATGCTATT GTCAATTTTG CTCGAATTGT TCCCAATGGA 
TTGCTAGTAT TCTTTCCGTC GTACTACATT CTGGACCAAT GCATTGCCTG CTGGAAGAAA 
ATGAGCCATG CCAATTCAAC TACAACATGG GAAAGAATCT CTAAACATAA GAAACCTGTT 
GTGGAACCTA GACAATCTTC GTTGTTTCTT TTGTCAATTG AGGATTATAT GGCAAAGCTG 
AAGGACACCT CAGCTTCTGG TGCAGTATTT TTTGCTGTTT GCCGTGGTAA AGTAAGTGAA 
GGACTAGATT TTGCTGATCA TGCTGGAAGA GCTGTAGTCA TTACTGGTCT ACCATTTGCC 
ACAAGGAATG ATCCTAAGGT TCGACTGAAA CGTGAATACT TGGATCAGCA GGCATATTCC 
CAAAGGCAAG CATGTAAGTT GAAGGTTCTA ACTGGAGAAG ACTGGTACAA TCAACAAGCA 
TTACGAGCTG TGAATCAGGC TGTTGGGCGC GTAATCCGTC ATCGTCATGA TTATGGAGCA 
ATTATTTTTT GTGATGAAAG GTTTGCACAT TCAAACCGTC AATCTCAAAT ATCGCTCTGG 
ATACAACCTC ATATCAAGTG TTACTCTAAC TTTGGGGACG TAGTTTTTTC GTTGACCCGA 
TTCTTTCGGG ATGGAGATCG GGGTCCTACT AAGCTGCAAC AAACTGAAAA ATGGGATGCA 
ATTTCTACTT CAAAATCAGT ACTCCATGAG CAGCCCGATC ATAAACCATC TTCTCTAGGT 
TCATCAACTT CAGATCATGG AACCCCAGGG AAAATATATG AGTTGAAAAC TGAGGAACCA 
CCAGAAATAT TTTACTTGGA CCAGTTTCTA CCTAAGACAA CTGCAGTTGG TCAAGACCCC 
GCTGTAAAAT CGCCTTCTTT CCTACTTAAA GCTAGAAGGG ACAGAATATC AAGCCTGTTA 
GGCGAAGTCC TTCCTGCCAA CCGCTCATCT CTTACTTGCT GTAAGGATCA GTCTGTGTCA 
TTAAAGTATT CAAATGATCT ATTTCAAAGT GAGAAAAAGA TGCGTATGTG TGATAGGGGA 
ACTATGCAAT GTCAAGAAAG TAAGGTTCTT GGGTTTCCTG GGAAAAATGA GAAACGAAGT 
GAACAACAAC TGATAGCACC TTGTTCCACA AAGAAGTATA AATTAGAGCA TGGTGCAGAA 
CAACATGACA GTTCTAAGCA TTCATCTCTT GCAAAAGATG CTCACTCATA TGGTCTTTAC 
TCCACTAATA ATTCAATGAG AAGTGAGAGT TCACATAGAT CTGACAGTGG AAGTGCACAA 
AATGCTCAAG TTGGTTCAGC TCTGCTGTGC AAAGATACGA GGATCATACA ACAGGGAGAT 
GCTGAGTTTC TGAGCAAGAA AAAAACGAGT GCAATATCCA GCCCTGTGCC TTCTGGTGAT 
GGGGAAACCA GAGGATCTGA GTTTCTAGTT CAGGTTGAGG AAAAACTTAG TGCTTTGGAA 
TATAAAGAAT TTGTAAATTT GATGAAGGCA CTTAAGTCCA AAGCTATGAA GATAAATGAG 
GTTTTGCAAT CCATTGCGGG CTTGTTTTCC GGAAGAGAGA GGCTTCCTCT TCTTAAAAGA 
TTTAAGGACT TTATCCCTGC AAAGTATCAC TCTATGTATG AGAACTATTT TCAAACAAAT 
GACGGATCTC TTGATATCCA AGGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028953420.1
CDS	135..3344
Translation	MPTYNIRGIDVDFPFEAYDCQLVYMEKVIQSLQEKSNALLESPTGTGKTLCLLCATLAWRKSLGPFSTGPNVKMSVITGDASGGPSSQSVTKGFPTIVYTSRTHSQIRQVIQELKRSAYRPKMVVLGSREQLCIDEEVSLLRGRTQTKACHVLCRKRTKDTKRRCSHYSRGSGFLKSNPHLGDEPVDIEDLVNTGKKLGPCPYYLSRELHKVVDILFAPYNYLIDPAFRKSLQVDWKNSILIFDEAHNLESICADAASFDLPSWLLTTCISEAKNCIDLSSKRREDSTDKSQNPDDFAILRALLLKLEKRISEVPIESKELGYTKPGPYIYELLADLNISHDTVPKLIAILEDATVLIEEDNQDKANRNACRLESISDMLNIIFRDKDNNHAKSYRVHVQEVEASAIDGLKGKASRTLSWWCFHPGIAMEEFARIGVGSIILTSGTLSPLDSFAQELKLEFPVRLENPHVITPNQIWAGVVPAGPSGFSFNSSYRNRDSTEYKQDLGNAIVNFARIVPNGLLVFFPSYYILDQCIACWKKMSHANSTTTWERISKHKKPVVEPRQSSLFLLSIEDYMAKLKDTSASGAVFFAVCRGKVSEGLDFADHAGRAVVITGLPFATRNDPKVRLKREYLDQQAYSQRQACKLKVLTGEDWYNQQALRAVNQAVGRVIRHRHDYGAIIFCDERFAHSNRQSQISLWIQPHIKCYSNFGDVVFSLTRFFRDGDRGPTKLQQTEKWDAISTSKSVLHEQPDHKPSSLGSSTSDHGTPGKIYELKTEEPPEIFYLDQFLPKTTAVGQDPAVKSPSFLLKARRDRISSLLGEVLPANRSSLTCCKDQSVSLKYSNDLFQSEKKMRMCDRGTMQCQESKVLGFPGKNEKRSEQQLIAPCSTKKYKLEHGAEQHDSSKHSSLAKDAHSYGLYSTNNSMRSESSHRSDSGSAQNAQVGSALLCKDTRIIQQGDAEFLSKKKTSAISSPVPSGDGETRGSEFLVQVEEKLSALEYKEFVNLMKALKSKAMKINEVLQSIAGLFSGRERLPLLKRFKDFIPAKYHSMYENYFQTNDGSLDIQGK

Target ORF information:

RefSeq Version	XM_029097587.1
Organism	Malus domestica(apple)
Definition	Malus domestica regulator of telomere elongation helicase 1 homolog (LOC103405483), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029097587.1

ORF Insert Sequence:

ATGCCGACGT ACAACATCAG AGGCATCGAT GTCGATTTCC CATTTGAGGC GTACGATTGC 
CAGCTCGTTT ACATGGAGAA AGTCATTCAA TCTCTCCAAG AAAAAAGTAA TGCACTGCTG 
GAGAGTCCCA CTGGAACTGG GAAGACCCTT TGTCTTCTGT GCGCCACATT GGCCTGGAGG 
AAGAGTTTGG GCCCTTTCTC AACTGGTCCT AATGTCAAGA TGAGTGTGAT CACAGGAGAT 
GCATCAGGTG GTCCCTCGTC ACAGTCTGTG ACAAAAGGTT TTCCTACCAT AGTATATACG 
TCCCGCACTC ACAGCCAGAT CCGGCAAGTA ATTCAAGAGT TGAAAAGAAG CGCTTACAGG 
CCAAAAATGG TAGTGTTGGG ATCTCGAGAG CAATTATGCA TTGATGAGGA AGTGAGTTTA 
CTTCGCGGAA GAACACAGAC AAAGGCTTGC CATGTCCTCT GCAGAAAGCG CACAAAGGAT 
ACAAAGCGTC GTTGTTCTCA TTACTCTCGT GGTTCTGGTT TCTTGAAGAG TAATCCTCAT 
CTTGGAGATG AACCTGTTGA TATCGAGGAT TTGGTCAATA CTGGAAAAAA ATTGGGGCCG 
TGTCCTTATT ATTTATCGAG GGAGCTCCAT AAGGTTGTTG ATATATTATT TGCACCTTAT 
AACTATCTCA TTGATCCTGC ATTTAGAAAA TCGCTACAGG TTGATTGGAA AAACAGTATT 
CTGATATTTG ACGAAGCTCA CAACCTGGAA AGCATATGTG CAGATGCTGC TTCCTTTGAC 
TTGCCCTCTT GGCTTCTTAC AACTTGCATT TCTGAAGCAA AAAATTGTAT TGACCTTTCT 
TCAAAAAGAA GGGAAGACTC AACTGATAAA TCACAGAATC CAGATGACTT TGCTATCCTC 
AGAGCGCTAC TATTGAAGCT TGAGAAGCGT ATTTCTGAAG TACCTATCGA ATCCAAGGAA 
TTGGGATACA CAAAGCCTGG GCCTTATATC TATGAACTCC TGGCGGATTT AAACATCTCG 
CATGATACTG TCCCTAAGCT CATTGCAATA CTTGAGGACG CTACTGTACT TATTGAGGAA 
GATAACCAGG ACAAGGCGAA TCGAAATGCC TGCCGGCTGG AAAGCATTAG TGACATGCTT 
AATATAATTT TCAGAGATAA AGACAATAAT CATGCGAAAT CCTATCGTGT TCATGTACAA 
GAAGTTGAAG CCAGTGCAAT AGATGGTTTA AAGGGGAAGG CTTCTAGGAC ACTTAGCTGG 
TGGTGTTTTC ATCCTGGAAT TGCAATGGAA GAATTTGCCA GGATAGGTGT GGGCTCTATT 
ATACTGACAT CTGGCACATT ATCTCCCTTG GATTCGTTTG CACAGGAACT GAAACTAGAG 
TTTCCTGTGC GGCTGGAAAA TCCTCATGTT ATTACCCCGA ATCAGATATG GGCTGGAGTT 
GTACCAGCTG GCCCTTCTGG ATTCTCTTTC AACTCCTCTT ACAGAAATCG TGATTCTACG 
GAGTACAAGC AGGACCTTGG AAATGCTATT GTCAATTTTG CTCGAATTGT TCCCAATGGA 
TTGCTAGTAT TCTTTCCGTC GTACTACATT CTGGACCAAT GCATTGCCTG CTGGAAGAAA 
ATGAGCCATG CCAATTCAAC TACAACATGG GAAAGAATCT CTAAACATAA GAAACCTGTT 
GTGGAACCTA GACAATCTTC GTTGTTTCTT TTGTCAATTG AGGATTATAT GGCAAAGCTG 
AAGGACACCT CAGCTTCTGG TGCAGTATTT TTTGCTGTTT GCCGTGGTAA AGTAAGTGAA 
GGACTAGATT TTGCTGATCA TGCTGGAAGA GCTGTAGTCA TTACTGGTCT ACCATTTGCC 
ACAAGGAATG ATCCTAAGGT TCGACTGAAA CGTGAATACT TGGATCAGCA GGCATATTCC 
CAAAGGCAAG CATGTAAGTT GAAGGTTCTA ACTGGAGAAG ACTGGTACAA TCAACAAGCA 
TTACGAGCTG TGAATCAGGC TGTTGGGCGC GTAATCCGTC ATCGTCATGA TTATGGAGCA 
ATTATTTTTT GTGATGAAAG GTTTGCACAT TCAAACCGTC AATCTCAAAT ATCGCTCTGG 
ATACAACCTC ATATCAAGTG TTACTCTAAC TTTGGGGACG TAGTTTTTTC GTTGACCCGA 
TTCTTTCGGG ATGGAGATCG GGGTCCTACT AAGCTGCAAC AAACTGAAAA ATGGGATGCA 
ATTTCTACTT CAAAATCAGT ACTCCATGAG CAGCCCGATC ATAAACCATC TTCTCTAGGT 
TCATCAACTT CAGATCATGG AACCCCAGGG AAAATATATG AGTTGAAAAC TGAGGAACCA 
CCAGAAATAT TTTACTTGGA CCAGTTTCTA CCTAAGACAA CTGCAGTTGG TCAAGACCCC 
GCTGTAAAAT CGCCTTCTTT CCTACTTAAA GCTAGAAGGG ACAGAATATC AAGCCTGTTA 
GGCGAAGTCC TTCCTGCCAA CCGCTCATCT CTTACTTGCT GTAAGGATCA GTCTGTGTCA 
TTAAAGTATT CAAATGATCT ATTTCAAAGT GAGAAAAAGA TGCGTATGTG TGATAGGGGA 
ACTATGCAAT GTCAAGAAAG TAAGGTTCTT GGGTTTCCTG GGAAAAATGA GAAACGAAGT 
GAACAACAAC TGATAGCACC TTGTTCCACA AAGAAGTATA AATTAGAGCA TGGTGCAGAA 
CAACATGACA GTTCTAAGCA TTCATCTCTT GCAAAAGATG CTCACTCATA TGGTCTTTAC 
TCCACTAATA ATTCAATGAG AAGTGAGAGT TCACATAGAT CTGACAGTGG AAGTGCACAA 
AATGCTCAAG TTGGTTCAGC TCTGCTGTGC AAAGATACGA GGATCATACA ACAGGGAGAT 
GCTGAGTTTC TGAGCAAGAA AAAAACGAGT GCAATATCCA GCCCTGTGCC TTCTGGTGAT 
GGGGAAACCA GAGGATCTGA GTTTCTAGTT CAGGTTGAGG AAAAACTTAG TGCTTTGGAA 
TATAAAGAAT TTGTAAATTT GATGAAGGCA CTTAAGTCCA AAGCTATGAA GATAAATGAG 
GTTTTGCAAT CCATTGCGGG CTTGTTTTCC GGAAGAGAGA GGCTTCCTCT TCTTAAAAGA 
TTTAAGGACT TTATCCCTGC AAAGTATCAC TCTATGTATG AGAACTATTT TCAAACAAAT 
GACGGATCTC TTGATATCCA AGGAAAGTAA