LOC111689680 cDNA ORF clone, Lucilia cuprina(Australian sheep blowfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111689680
Entrez Gene ID	111689680
Full Name	2-oxoglutarate dehydrogenase-like, mitochondrial
Gene Type	protein-coding
Organism	Lucilia cuprina(Australian sheep blowfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023452151.1	XP_023307919.1	2-oxoglutarate dehydrogenase-like, mitochondrial isoform X1
XM_023452152.1	XP_023307920.1	2-oxoglutarate dehydrogenase-like, mitochondrial isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC111689680 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111689680 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLu60158	XM_023452151.1 Latest version!	Lucilia cuprina 2-oxoglutarate dehydrogenase-like, mitochondrial (LOC111689680), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OLu60159	XM_023452152.1 Latest version!	Lucilia cuprina 2-oxoglutarate dehydrogenase-like, mitochondrial (LOC111689680), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111689680 ( XM_023452151.1 ) cDNA ORF clone, Lucilia cuprina(Australian sheep blowfly) -> XP_023307919.1 Lucilia cuprina 2-oxoglutarate dehydrogenase-like, mitochondrial (LOC111689680), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLu60158
Clone ID Related Accession (Same CDS sequence)	XM_023452151.1
Accession Version	XM_023452151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-04
Organism	Lucilia cuprina(Australian sheep blowfly)
Product	2-oxoglutarate dehydrogenase-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019412701.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Lucilia cuprina Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGGG AATTTAAATT ACCCAGCACT ACATTCATTG GTGGAGAAGA AAATCAATTG 
CCCCTAAGAG AAATTTTGAA TCGTTTGGAA AATGTATATT GTAATAAAAT TGGTGTTGAA 
TTCATGTTCA TCAATTCATT GGAACAGTGC AATTGGATTC GTAAACGTTT TGAAACTCCT 
GGCGTTATGA ATTTCTCACC TGAAAAGAAA CGTTTGATTT TGGCTCGTTT GACACGTGCT 
ACTGGATTTG AAGGTTTCTT GGCTAAGAAA TACTCTTCGG AGAAACGTTT TGGTTTGGAG 
GGTTGTGAAA TTATGATTCC CGCTTTGAAG GAACTTATCG ATGCCTCTAC AGAGTTGGGT 
GTTGAATCTG TGATTATGGG TATGCCCCAT CGTGGTCGTT TGAATACATT GGCCAATGTA 
TGCCGCAAAC CTTTACATCA AATCTTTACA CAGTTTGCTG GTTTGGAAGC TGCTGATGAT 
GGTTCCGGTG ATGTAAAATA CCATTTAGGT ACATACATTG AACGTTTGAA CCGTGTCACA 
AACAAGAATA TTCGTTTGGC TGTTGTTGCT AATCCCTCTC ACTTGGAAGC CGTCGACCCT 
GTTGTACAGG GTAAGACTCG TGCTGAACAG TTCTACCGTG GTGATCAAGA AGGCAATAAG 
GTTATGTCTA TTTTGATTCA CGGTGATGCT GCTTTCTGTG GTCAAGGTGT TGTCTATGAA 
ACTATGCACT TGTCTGATTT GCCCGACTAC ACTACTCATG GTACAATTCA CGTTGTTGCT 
AATAACCAAA TTGGTTTCAC TACTGATCCA CGTTTCTCAC GCTCCTCACC ATACTGTACC 
GATGTTGCTC GTGTTGTGAA TGCTCCCATT TTCCACGTCA ACGCCGATGA TCCCGAGGCT 
GTTATGCATG TGTGCAGAGT TGCCGCCGAA TGGCGTAACA CTTTCCACAA GGATGTCGTT 
ATCGATTTGG TCGGCTATCG TCGTAATGGC CACAACGAAA TTGATGAACC CATGTTCACC 
CAACCTCTTA TGTACCAAAA GATCAGAAAC CACAAAATCT GTTTGGATCT CTATGCCGAT 
AAACTTATCG CCGAAGGCAC TTGCACCGCT GAAGAAGTTA AATCAGTGGC TGATAAATAC 
GAAAAGATTT GCGAAGAAGC CTTCGAATTG GCTAAGAAGG AAACACATAT TAAGTACAAG 
GACTGGATCG ATTCTCCCTG GTCTGGTTTC TTTGAAGGCA AGGATCCCTT GAAGGTATCA 
CCCACTGGTG TAAAGATTGA AACCTTGGTA CACATTGGCA ACAAATTCTC TTCACCACCA 
CCAAATGCTG CTGAATTCGT AATTCACAAG GGTCTTACTC GTGTTTTGAA TGCCCGCAAG 
GCTATGGTTG ATGAAGGTGT TGCCGATTGG GCTCTTGGTG AAGCCATGGC CTTTGGCTCT 
CTCCTTAAGG AAGGCGTTCA TGTCCGTTTG TCTGGTCAAG ATGTTGAACG TGGTACCTTC 
TCTCATCGTC ATCATGTTTT GCACCATCAA TTGGTTGACA AGGCTACTTA TAATGCCCTC 
CAACACTTGT ACCCCGATCA AGCACCTTAC TCCGTTTCGA ACAGTTCATT GTCTGAATAC 
GCTGTACTTG GTTTTGAACA CGGCTACTCC ATGACTAATC CCAATGCCTT GGTCTTATGG 
GAAGCTCAAT TCGGTGATTT CTTTAATACA GCTCAATGTA TTGTCGATCA ATTCCTTTCC 
AGTGGCCAAG CTAAGTGGGT ACGTCAATCC GGTTTGGTAA TGTTGTTGCC TCACGGTATG 
GAAGGCATGG GTCCTGAACA TTCATCGGCT CGCGTTGAAC GTTTCTTGCA AATGTCTTCT 
GACGATCCCG ACTATTTCCC TCCAGAATCT GAGGAATTCG CTATCAGACA ATTGCACGAT 
ATTAATTGGA TTGTTGCCAA TTGCACTACC CCTGCTAACT TGTTCCACAT TTTGCGTCGT 
CAAATTGCTT TGCCCTTCCG CAAACCCTTG GTACTCCTTA CTCCCAAATC TCTATTGCGT 
CACCCAGAAG CCAAGTCACC TTTCAGTGAT ATGGCTGAAG GCAGTGAATT CCAACGTATC 
ATCCCCGAAA AGGGAGTTGC TGCTGAAAAC CCCAGCGGTG TTAAGAAGGT TATTTTCTGC 
AGCGGTCGTG TATACTATGA CTTGACCAAA CAACGCAAGG ACAAGGGCTT GGAAAAGGAA 
ATCGCTATTG TTCGTGTTGA ACAAATTTCT CCCTTCCCCT TCGATCTAGT CAAAGAACAA 
GCTAACTTGT ACAAGGATGC TGATTTGGTT TGGGCTCAAG AAGAACACAA AAATCAAGGC 
TGCTGGTCTT ATGTGCAACC ACGTTTCTTG ACCGCCCTTA ACCACGATAG AAATATTAGC 
CCCCATGATG CTACTTCCTC TTCCACTACT ACTACCACTA CCAATACTAA TGAGTCCGAT 
TCCGCTAATC AATCGAAACC CTGGTTAAGT CGTATGTTTA CATCCGGTGG TAGCAAGTCT 
GCTGATAATG ATGCCTCCTC TGGCAATCAA CAGCAGACTG GTGAATATTG TGAGAAGAAA 
CATTTTGATT TGAAAAATGT ACGCAAAGAT TTTAATAGAC CCGCTGGTCA TTCTGCGGCA 
CCTGGTGTGC GTGTTTCTAA AACTGGACGC AAAATTAGCT ATGCTGGACG TCCATGTGGT 
GCTTCGACTG CTACCGGTTC TAAGGCTCAA CATATACGTG AATTGAATGC TTTGTTGGCT 
GATGCCATCA GTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023307919.1
CDS	10..2787
Translation	MDREFKLPSTTFIGGEENQLPLREILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVMNFSPEKKRLILARLTRATGFEGFLAKKYSSEKRFGLEGCEIMIPALKELIDASTELGVESVIMGMPHRGRLNTLANVCRKPLHQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGNKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCRVAAEWRNTFHKDVVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRNHKICLDLYADKLIAEGTCTAEEVKSVADKYEKICEEAFELAKKETHIKYKDWIDSPWSGFFEGKDPLKVSPTGVKIETLVHIGNKFSSPPPNAAEFVIHKGLTRVLNARKAMVDEGVADWALGEAMAFGSLLKEGVHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNALQHLYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFFNTAQCIVDQFLSSGQAKWVRQSGLVMLLPHGMEGMGPEHSSARVERFLQMSSDDPDYFPPESEEFAIRQLHDINWIVANCTTPANLFHILRRQIALPFRKPLVLLTPKSLLRHPEAKSPFSDMAEGSEFQRIIPEKGVAAENPSGVKKVIFCSGRVYYDLTKQRKDKGLEKEIAIVRVEQISPFPFDLVKEQANLYKDADLVWAQEEHKNQGCWSYVQPRFLTALNHDRNISPHDATSSSTTTTTTNTNESDSANQSKPWLSRMFTSGGSKSADNDASSGNQQQTGEYCEKKHFDLKNVRKDFNRPAGHSAAPGVRVSKTGRKISYAGRPCGASTATGSKAQHIRELNALLADAISI

Target ORF information:

RefSeq Version	XM_023452151.1
Organism	Lucilia cuprina(Australian sheep blowfly)
Definition	Lucilia cuprina 2-oxoglutarate dehydrogenase-like, mitochondrial (LOC111689680), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023452151.1

ORF Insert Sequence:

ATGGATCGGG AATTTAAATT ACCCAGCACT ACATTCATTG GTGGAGAAGA AAATCAATTG 
CCCCTAAGAG AAATTTTGAA TCGTTTGGAA AATGTATATT GTAATAAAAT TGGTGTTGAA 
TTCATGTTCA TCAATTCATT GGAACAGTGC AATTGGATTC GTAAACGTTT TGAAACTCCT 
GGCGTTATGA ATTTCTCACC TGAAAAGAAA CGTTTGATTT TGGCTCGTTT GACACGTGCT 
ACTGGATTTG AAGGTTTCTT GGCTAAGAAA TACTCTTCGG AGAAACGTTT TGGTTTGGAG 
GGTTGTGAAA TTATGATTCC CGCTTTGAAG GAACTTATCG ATGCCTCTAC AGAGTTGGGT 
GTTGAATCTG TGATTATGGG TATGCCCCAT CGTGGTCGTT TGAATACATT GGCCAATGTA 
TGCCGCAAAC CTTTACATCA AATCTTTACA CAGTTTGCTG GTTTGGAAGC TGCTGATGAT 
GGTTCCGGTG ATGTAAAATA CCATTTAGGT ACATACATTG AACGTTTGAA CCGTGTCACA 
AACAAGAATA TTCGTTTGGC TGTTGTTGCT AATCCCTCTC ACTTGGAAGC CGTCGACCCT 
GTTGTACAGG GTAAGACTCG TGCTGAACAG TTCTACCGTG GTGATCAAGA AGGCAATAAG 
GTTATGTCTA TTTTGATTCA CGGTGATGCT GCTTTCTGTG GTCAAGGTGT TGTCTATGAA 
ACTATGCACT TGTCTGATTT GCCCGACTAC ACTACTCATG GTACAATTCA CGTTGTTGCT 
AATAACCAAA TTGGTTTCAC TACTGATCCA CGTTTCTCAC GCTCCTCACC ATACTGTACC 
GATGTTGCTC GTGTTGTGAA TGCTCCCATT TTCCACGTCA ACGCCGATGA TCCCGAGGCT 
GTTATGCATG TGTGCAGAGT TGCCGCCGAA TGGCGTAACA CTTTCCACAA GGATGTCGTT 
ATCGATTTGG TCGGCTATCG TCGTAATGGC CACAACGAAA TTGATGAACC CATGTTCACC 
CAACCTCTTA TGTACCAAAA GATCAGAAAC CACAAAATCT GTTTGGATCT CTATGCCGAT 
AAACTTATCG CCGAAGGCAC TTGCACCGCT GAAGAAGTTA AATCAGTGGC TGATAAATAC 
GAAAAGATTT GCGAAGAAGC CTTCGAATTG GCTAAGAAGG AAACACATAT TAAGTACAAG 
GACTGGATCG ATTCTCCCTG GTCTGGTTTC TTTGAAGGCA AGGATCCCTT GAAGGTATCA 
CCCACTGGTG TAAAGATTGA AACCTTGGTA CACATTGGCA ACAAATTCTC TTCACCACCA 
CCAAATGCTG CTGAATTCGT AATTCACAAG GGTCTTACTC GTGTTTTGAA TGCCCGCAAG 
GCTATGGTTG ATGAAGGTGT TGCCGATTGG GCTCTTGGTG AAGCCATGGC CTTTGGCTCT 
CTCCTTAAGG AAGGCGTTCA TGTCCGTTTG TCTGGTCAAG ATGTTGAACG TGGTACCTTC 
TCTCATCGTC ATCATGTTTT GCACCATCAA TTGGTTGACA AGGCTACTTA TAATGCCCTC 
CAACACTTGT ACCCCGATCA AGCACCTTAC TCCGTTTCGA ACAGTTCATT GTCTGAATAC 
GCTGTACTTG GTTTTGAACA CGGCTACTCC ATGACTAATC CCAATGCCTT GGTCTTATGG 
GAAGCTCAAT TCGGTGATTT CTTTAATACA GCTCAATGTA TTGTCGATCA ATTCCTTTCC 
AGTGGCCAAG CTAAGTGGGT ACGTCAATCC GGTTTGGTAA TGTTGTTGCC TCACGGTATG 
GAAGGCATGG GTCCTGAACA TTCATCGGCT CGCGTTGAAC GTTTCTTGCA AATGTCTTCT 
GACGATCCCG ACTATTTCCC TCCAGAATCT GAGGAATTCG CTATCAGACA ATTGCACGAT 
ATTAATTGGA TTGTTGCCAA TTGCACTACC CCTGCTAACT TGTTCCACAT TTTGCGTCGT 
CAAATTGCTT TGCCCTTCCG CAAACCCTTG GTACTCCTTA CTCCCAAATC TCTATTGCGT 
CACCCAGAAG CCAAGTCACC TTTCAGTGAT ATGGCTGAAG GCAGTGAATT CCAACGTATC 
ATCCCCGAAA AGGGAGTTGC TGCTGAAAAC CCCAGCGGTG TTAAGAAGGT TATTTTCTGC 
AGCGGTCGTG TATACTATGA CTTGACCAAA CAACGCAAGG ACAAGGGCTT GGAAAAGGAA 
ATCGCTATTG TTCGTGTTGA ACAAATTTCT CCCTTCCCCT TCGATCTAGT CAAAGAACAA 
GCTAACTTGT ACAAGGATGC TGATTTGGTT TGGGCTCAAG AAGAACACAA AAATCAAGGC 
TGCTGGTCTT ATGTGCAACC ACGTTTCTTG ACCGCCCTTA ACCACGATAG AAATATTAGC 
CCCCATGATG CTACTTCCTC TTCCACTACT ACTACCACTA CCAATACTAA TGAGTCCGAT 
TCCGCTAATC AATCGAAACC CTGGTTAAGT CGTATGTTTA CATCCGGTGG TAGCAAGTCT 
GCTGATAATG ATGCCTCCTC TGGCAATCAA CAGCAGACTG GTGAATATTG TGAGAAGAAA 
CATTTTGATT TGAAAAATGT ACGCAAAGAT TTTAATAGAC CCGCTGGTCA TTCTGCGGCA 
CCTGGTGTGC GTGTTTCTAA AACTGGACGC AAAATTAGCT ATGCTGGACG TCCATGTGGT 
GCTTCGACTG CTACCGGTTC TAAGGCTCAA CATATACGTG AATTGAATGC TTTGTTGGCT 
GATGCCATCA GTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC111689680 ( XM_023452152.1 ) cDNA ORF clone, Lucilia cuprina(Australian sheep blowfly) -> XP_023307920.1 Lucilia cuprina 2-oxoglutarate dehydrogenase-like, mitochondrial (LOC111689680), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLu60159
Clone ID Related Accession (Same CDS sequence)	XM_023452152.1
Accession Version	XM_023452152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-04
Organism	Lucilia cuprina(Australian sheep blowfly)
Product	2-oxoglutarate dehydrogenase-like, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019412701.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Lucilia cuprina Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGGG AATTTAAATT ACCCAGCACT ACATTCATTG GTGGAGAAGA AAATCAATTG 
CCCCTAAGAG AAATTTTGAA TCGTTTGGAA AATGTATATT GTAATAAAAT TGGTGTTGAA 
TTCATGTTCA TCAATTCATT GGAACAGTGC AATTGGATTC GTAAACGTTT TGAAACTCCT 
GGCGTTATGA ATTTCTCACC TGAAAAGAAA CGTTTGATTT TGGCTCGTTT GACACGTGCT 
ACTGGATTTG AAGGTTTCTT GGCTAAGAAA TACTCTTCGG AGAAACGTTT TGGTTTGGAG 
GGTTGTGAAA TTATGATTCC CGCTTTGAAG GAACTTATCG ATGCCTCTAC AGAGTTGGGT 
GTTGAATCTG TGATTATGGG TATGCCCCAT CGTGGTCGTT TGAATACATT GGCCAATGTA 
TGCCGCAAAC CTTTACATCA AATCTTTACA CAGTTTGCTG GTTTGGAAGC TGCTGATGAT 
GGTTCCGGTG ATGTAAAATA CCATTTAGGT ACATACATTG AACGTTTGAA CCGTGTCACA 
AACAAGAATA TTCGTTTGGC TGTTGTTGCT AATCCCTCTC ACTTGGAAGC CGTCGACCCT 
GTTGTACAGG GTAAGACTCG TGCTGAACAG TTCTACCGTG GTGATCAAGA AGGCAATAAG 
GTTATGTCTA TTTTGATTCA CGGTGATGCT GCTTTCTGTG GTCAAGGTGT TGTCTATGAA 
ACTATGCACT TGTCTGATTT GCCCGACTAC ACTACTCATG GTACAATTCA CGTTGTTGCT 
AATAACCAAA TTGGTTTCAC TACTGATCCA CGTTTCTCAC GCTCCTCACC ATACTGTACC 
GATGTTGCTC GTGTTGTGAA TGCTCCCATT TTCCACGTCA ACGCCGATGA TCCCGAGGCT 
GTTATGCATG TGTGCAGAGT TGCCGCCGAA TGGCGTAACA CTTTCCACAA GGATGTCGTT 
ATCGATTTGG TCGGCTATCG TCGTAATGGC CACAACGAAA TTGATGAACC CATGTTCACC 
CAACCTCTTA TGTACCAAAA GATCAGAAAC CACAAAATCT GTTTGGATCT CTATGCCGAT 
AAACTTATCG CCGAAGGCAC TTGCACCGCT GAAGAAGTTA AATCAGTGGC TGATAAATAC 
GAAAAGATTT GCGAAGAAGC CTTCGAATTG GCTAAGAAGG AAACACATAT TAAGTACAAG 
GACTGGATCG ATTCTCCCTG GTCTGGTTTC TTTGAAGGCA AGGATCCCTT GAAGGTATCA 
CCCACTGGTG TAAAGATTGA AACCTTGGTA CACATTGGCA ACAAATTCTC TTCACCACCA 
CCAAATGCTG CTGAATTCGT AATTCACAAG GGTCTTACTC GTGTTTTGAA TGCCCGCAAG 
GCTATGGTTG ATGAAGGTGT TGCCGATTGG GCTCTTGGTG AAGCCATGGC CTTTGGCTCT 
CTCCTTAAGG AAGGCGTTCA TGTCCGTTTG TCTGGTCAAG ATGTTGAACG TGGTACCTTC 
TCTCATCGTC ATCATGTTTT GCACCATCAA TTGGTTGACA AGGCTACTTA TAATGCCCTC 
CAACACTTGT ACCCCGATCA AGCACCTTAC TCCGTTTCGA ACAGTTCATT GTCTGAATAC 
GCTGTACTTG GTTTTGAACA CGGCTACTCC ATGACTAATC CCAATGCCTT GGTCTTATGG 
GAAGCTCAAT TCGGTGATTT CTTTAATACA GCTCAATGTA TTGTCGATCA ATTCCTTTCC 
AGTGGCCAAG CTAAGTGGGT ACGTCAATCC GGTTTGGTAA TGTTGTTGCC TCACGGTATG 
GAAGGCATGG GTCCTGAACA TTCATCGGCT CGCGTTGAAC GTTTCTTGCA AATGTCTTCT 
GACGATCCCG ACTATTTCCC TCCAGAATCT GAGGAATTCG CTATCAGACA ATTGCACGAT 
ATTAATTGGA TTGTTGCCAA TTGCACTACC CCTGCTAACT TGTTCCACAT TTTGCGTCGT 
CAAATTGCTT TGCCCTTCCG CAAACCCTTG GTACTCCTTA CTCCCAAATC TCTATTGCGT 
CACCCAGAAG CCAAGTCACC TTTCAGTGAT ATGGCTGAAG GCAGTGAATT CCAACGTATC 
ATCCCCGAAA AGGGAGTTGC TGCTGAAAAC CCCAGCGGTG TTAAGAAGGT TATTTTCTGC 
AGCGGTCGTG TATACTATGA CTTGACCAAA CAACGCAAGG ACAAGGGCTT GGAAAAGGAA 
ATCGCTATTG TTCGTGTTGA ACAAATTTCT CCCTTCCCCT TCGATCTAGT CAAAGAACAA 
GCTAACTTGT ACAAGGATGC TGATTTGGTT TGGGCTCAAG AAGAACACAA AAATCAAGGC 
TGCTGGTCTT ATGTGCAACC ACGTTTCTTG ACCGCCCTTA ACCACGATAG AAATATTAGC 
TATGCTGGAC GTCCATGTGG TGCTTCGACT GCTACCGGTT CTAAGGCTCA ACATATACGT 
GAATTGAATG CTTTGTTGGC TGATGCCATC AGTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023307920.1
CDS	10..2508
Translation	MDREFKLPSTTFIGGEENQLPLREILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVMNFSPEKKRLILARLTRATGFEGFLAKKYSSEKRFGLEGCEIMIPALKELIDASTELGVESVIMGMPHRGRLNTLANVCRKPLHQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGNKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCRVAAEWRNTFHKDVVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRNHKICLDLYADKLIAEGTCTAEEVKSVADKYEKICEEAFELAKKETHIKYKDWIDSPWSGFFEGKDPLKVSPTGVKIETLVHIGNKFSSPPPNAAEFVIHKGLTRVLNARKAMVDEGVADWALGEAMAFGSLLKEGVHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNALQHLYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFFNTAQCIVDQFLSSGQAKWVRQSGLVMLLPHGMEGMGPEHSSARVERFLQMSSDDPDYFPPESEEFAIRQLHDINWIVANCTTPANLFHILRRQIALPFRKPLVLLTPKSLLRHPEAKSPFSDMAEGSEFQRIIPEKGVAAENPSGVKKVIFCSGRVYYDLTKQRKDKGLEKEIAIVRVEQISPFPFDLVKEQANLYKDADLVWAQEEHKNQGCWSYVQPRFLTALNHDRNISYAGRPCGASTATGSKAQHIRELNALLADAISI

Target ORF information:

RefSeq Version	XM_023452152.1
Organism	Lucilia cuprina(Australian sheep blowfly)
Definition	Lucilia cuprina 2-oxoglutarate dehydrogenase-like, mitochondrial (LOC111689680), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023452152.1

ORF Insert Sequence:

ATGGATCGGG AATTTAAATT ACCCAGCACT ACATTCATTG GTGGAGAAGA AAATCAATTG 
CCCCTAAGAG AAATTTTGAA TCGTTTGGAA AATGTATATT GTAATAAAAT TGGTGTTGAA 
TTCATGTTCA TCAATTCATT GGAACAGTGC AATTGGATTC GTAAACGTTT TGAAACTCCT 
GGCGTTATGA ATTTCTCACC TGAAAAGAAA CGTTTGATTT TGGCTCGTTT GACACGTGCT 
ACTGGATTTG AAGGTTTCTT GGCTAAGAAA TACTCTTCGG AGAAACGTTT TGGTTTGGAG 
GGTTGTGAAA TTATGATTCC CGCTTTGAAG GAACTTATCG ATGCCTCTAC AGAGTTGGGT 
GTTGAATCTG TGATTATGGG TATGCCCCAT CGTGGTCGTT TGAATACATT GGCCAATGTA 
TGCCGCAAAC CTTTACATCA AATCTTTACA CAGTTTGCTG GTTTGGAAGC TGCTGATGAT 
GGTTCCGGTG ATGTAAAATA CCATTTAGGT ACATACATTG AACGTTTGAA CCGTGTCACA 
AACAAGAATA TTCGTTTGGC TGTTGTTGCT AATCCCTCTC ACTTGGAAGC CGTCGACCCT 
GTTGTACAGG GTAAGACTCG TGCTGAACAG TTCTACCGTG GTGATCAAGA AGGCAATAAG 
GTTATGTCTA TTTTGATTCA CGGTGATGCT GCTTTCTGTG GTCAAGGTGT TGTCTATGAA 
ACTATGCACT TGTCTGATTT GCCCGACTAC ACTACTCATG GTACAATTCA CGTTGTTGCT 
AATAACCAAA TTGGTTTCAC TACTGATCCA CGTTTCTCAC GCTCCTCACC ATACTGTACC 
GATGTTGCTC GTGTTGTGAA TGCTCCCATT TTCCACGTCA ACGCCGATGA TCCCGAGGCT 
GTTATGCATG TGTGCAGAGT TGCCGCCGAA TGGCGTAACA CTTTCCACAA GGATGTCGTT 
ATCGATTTGG TCGGCTATCG TCGTAATGGC CACAACGAAA TTGATGAACC CATGTTCACC 
CAACCTCTTA TGTACCAAAA GATCAGAAAC CACAAAATCT GTTTGGATCT CTATGCCGAT 
AAACTTATCG CCGAAGGCAC TTGCACCGCT GAAGAAGTTA AATCAGTGGC TGATAAATAC 
GAAAAGATTT GCGAAGAAGC CTTCGAATTG GCTAAGAAGG AAACACATAT TAAGTACAAG 
GACTGGATCG ATTCTCCCTG GTCTGGTTTC TTTGAAGGCA AGGATCCCTT GAAGGTATCA 
CCCACTGGTG TAAAGATTGA AACCTTGGTA CACATTGGCA ACAAATTCTC TTCACCACCA 
CCAAATGCTG CTGAATTCGT AATTCACAAG GGTCTTACTC GTGTTTTGAA TGCCCGCAAG 
GCTATGGTTG ATGAAGGTGT TGCCGATTGG GCTCTTGGTG AAGCCATGGC CTTTGGCTCT 
CTCCTTAAGG AAGGCGTTCA TGTCCGTTTG TCTGGTCAAG ATGTTGAACG TGGTACCTTC 
TCTCATCGTC ATCATGTTTT GCACCATCAA TTGGTTGACA AGGCTACTTA TAATGCCCTC 
CAACACTTGT ACCCCGATCA AGCACCTTAC TCCGTTTCGA ACAGTTCATT GTCTGAATAC 
GCTGTACTTG GTTTTGAACA CGGCTACTCC ATGACTAATC CCAATGCCTT GGTCTTATGG 
GAAGCTCAAT TCGGTGATTT CTTTAATACA GCTCAATGTA TTGTCGATCA ATTCCTTTCC 
AGTGGCCAAG CTAAGTGGGT ACGTCAATCC GGTTTGGTAA TGTTGTTGCC TCACGGTATG 
GAAGGCATGG GTCCTGAACA TTCATCGGCT CGCGTTGAAC GTTTCTTGCA AATGTCTTCT 
GACGATCCCG ACTATTTCCC TCCAGAATCT GAGGAATTCG CTATCAGACA ATTGCACGAT 
ATTAATTGGA TTGTTGCCAA TTGCACTACC CCTGCTAACT TGTTCCACAT TTTGCGTCGT 
CAAATTGCTT TGCCCTTCCG CAAACCCTTG GTACTCCTTA CTCCCAAATC TCTATTGCGT 
CACCCAGAAG CCAAGTCACC TTTCAGTGAT ATGGCTGAAG GCAGTGAATT CCAACGTATC 
ATCCCCGAAA AGGGAGTTGC TGCTGAAAAC CCCAGCGGTG TTAAGAAGGT TATTTTCTGC 
AGCGGTCGTG TATACTATGA CTTGACCAAA CAACGCAAGG ACAAGGGCTT GGAAAAGGAA 
ATCGCTATTG TTCGTGTTGA ACAAATTTCT CCCTTCCCCT TCGATCTAGT CAAAGAACAA 
GCTAACTTGT ACAAGGATGC TGATTTGGTT TGGGCTCAAG AAGAACACAA AAATCAAGGC 
TGCTGGTCTT ATGTGCAACC ACGTTTCTTG ACCGCCCTTA ACCACGATAG AAATATTAGC 
TATGCTGGAC GTCCATGTGG TGCTTCGACT GCTACCGGTT CTAAGGCTCA ACATATACGT 
GAATTGAATG CTTTGTTGGC TGATGCCATC AGTATCTAA